Rorug02G0448000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005008
Physical Location & Seq
Forward (+)
57467708 .. 57472072
4365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0448000.1

Sequence Viewer

Length: 3297 bp
ATGAGTGACTTAGACTCAGCGCCCGGCCTAGTCTTCTTCATGTCTTCTTCTCTTGTTCCGATGAAACACGACAAGGAGCCAGAGGTCCCAACATATCCAAGAGTGGAACTGATCTTTTCTACTAAATGGGTTTTCGTTTTGCTGCTTCTACTTGTTCTTCTTCCTTGCTCTGTCTCTGCTGTCTGTAACAAAGTGGATCAAGATGCTCTTTTGTCTTTGGAGCTATCTTTCAAGGTTTCCTCCCCGTTGAATTGGTCTGTTTCTTCTGATTGTTGCCTCTGGGAAGGAATACTCTGCGGCCCCGATGATGATCGAGTTGTCAGTCTAACCCTGCCAGGGAGAGGATTAAGTGGTCCGATCAGTCCAGCTATCACAAACCTCACATTTCTCACCCACCTTAATCTCTCCCACAATTATCTTTCGGGTCTTCTACCCGATGGTTTGTTTCAATCCCTTCAAGTTCTGGACTTGAGCTTCAATCGGCTCAATGGCAATCTACCACTTTCATCTAACGAAACCAGCAAGCTTCAGATTGTAGACTTGTCAAGCAATTACTTCAATGGAACAATCCCATCTTCGATCCTCACCCCATCCGTCGCTGCAGGCTCTCTCGCCACTTTCAATGTCAGCAATAACAGCTTTGCTGGCTCCATTCCCATCTCTGAGTTCTGCAAAAATGGTAGCAATCACAGCAACCTCACCATCTTGGACTTCTCCTTCAACAAATTCAGTGGCCCAATTTCCACCGGACTCGGTTCTTGTTCGAAGCTCCAAGTCTTCCGGGCAGGCTTCAATAATCTGTCTGCTCCTCTCCCTGATGACATTTTCGACCTTGCTGATCTCCAACAGCTTTCTCTGCCTGTCAATCGCTTGGCAGGACACATTGGTGATGGCATTGTGCGTTTAACCAATCTGAAGGTGCTGGAGCTCTACTCGAACCAATTGGCGGGGCCAATACCAAGCCAAATTGGTAACCTTTTCAGGTTGGAACAGCTAGCTCTCCACATCAACAACCTCACAGGTGCCTTGCCTGCATCTCTCAACAACTGCACAAATCTATCCACCTTGAATTTGCGGGTCAATTATATGTCTGGACAACTCTCTGCCTTCAACTTCTCTACACTCCAACGCCTCACCACACTCGACCTTGGCAACAACAACTTCACTGGTGAGTTACCACAAAGCCTCTACTCTTGCAAGTCACTAACAGCAGTTAGATTCGCTAATAACCGGCTCATAGGCCAGATATCAGCTGAAATAGGTGGACTAGAGTCCCTGGCTTTCCTATCCATCTCTAACAACTTCTTAAGCAATGCCGCTGGGGCTTTTGAAAATTTAAGGAGTTGTAAGAATCTGACCACTCTGGTGCTATCCAAGAGTTTTATGAATGAACCCTTGCCGGATGATGAAAGCCTAGTAGACTCTGGTGGATTTCAGAATATACAGGTTTTTGCCCTGGGTGGCTGCAATTTCACAGGTCAAGTTCCTACCTGGCTTGGCAAGCTTAAGAAACTTGAAGTCTTGGACTTGTCAGTTAACCTCTTAACCGGTCCAATTCCCAGTTGGTTTGCTACTCTGCCTAGCCTTTTCTACATGGATTTGTCCAATAATAACCTTACTGGAGGATTTCCTAAGGAGCTCTGTGGGATGCCAGGTTTGACATCAAAAGAGGCCAGCAGTCTAGCAAAGAGCAGTAATCTAGAGTTGCCAGTATTTGTGATGCCTGAAAATGCAACTAATCAGCAGTACAATCAGCTGTCCAGCCTCCCTCCAGCTATATATCTTGGTAATAACAGCCTTAGCGGCAATATCCCCATTGAGATAGGGAAATTGCAGTTCATTCTTGTGTTGGACCTTAGTGATAACGGCTTCTCTGGCAGCATCCCGGTTCAGATATGTAATCTCACCAACTTGGAGAAATTGGATCTCTCCCACAACAGACTATCTGGTGAAATACCTGCTGCTCTCAAAGATTTGCATTTCATGTCTTCCTTCAATGTGGCATACAATGATCTTCAGGGACCCGTGCCATCTGGAGGTCAGTTTAATACTTTTACCAACTCGAGCTTTGAAGGGAATCCAGGATTATGTGGCCCTCAGACTGCACAGCACTCTTGCTCTCAATCAACAGCATCTCCACCATCAGCACCAGTTCGTAGTCGGAGGTCAAACAGAATCCTTCTGATTGGGCTTGCATCTTCTATTTGTTTTGGCATTGTTTTTATCATTGCAATGCTAGCTGTGTGGGTGTTGTCTAAGAGAAGAATTATTCCAGGAGGTGATTCTGACAAGATGGAATTGGATACAATCTCTAGCTATTCCACTGCTGCTGTTACTCCTGAGCTTGAGAAGGATACCAGCCTGGTCATCGTGTTCCCTACCAACACCAATGAAATCAAGGATCTTAATATATATGAAATACTGAAAGCAACCAACAACTTCAATCAAGCAAACATCATTGGCTGTGGAGGTTTTGGTCTGGTATACAAGGCAACATTAGCAAATGGAACCAATCTGGCTGTTAAGAAACTCTCAGGAGACTTGGGTTTGATGGAAAGGGAGTTCAAGGCGGAGGTTGAGGCACTTTCCACTGCTCAACATGAGAACCTTGTTTCACTGCAAGGGTATTGCGTGCATGATGGTGTTCGTCTATTAATGTATTCCTACATGGAGAATGGAAGCTTGGATTTCTGGTTACATGAGAAACCTGATGGTGCTTCCCAATTAGATTGGCCAACACGGCTGAAGATTGCACGGGGAGCAGGGGCTGGATTGGCTTACATGCATCAGATATGTGAGCCACACATTGTGCATCGTGATATCAAGTCCAGCAACATCCTGCTGGATGATAAATTCAAGGCACATGTTGCTGATTTTGGGTTGTCCCGGTTGATTCTTCCATATGAGACTCATGTTACAACCGAGCTTGTTGGTACCCTTGGTTACATTCCTCCCGAGTATGGACAAGCATGGGTGGCCACTTTAAGAGGAGATATGTACAGTTTTGGTGTTGTCATGCTTGAGCTGCTCACTGGAAAAAGGCCGTTTGAGGTATGCAAACCAAGGTCATCGGGAGATTTGGTTGGTTGGGTACAGCAAATGAGGAAGGAGGGCAAAGCTGAGGAAGTTTTTGATCCTCTCCTGAGAGAGAAAGGCTTTGAAGAAGAGATGCTGCAGGTGCTTGATGTAGCTTGCATGTGTGTCAACAGGAACCCTCTTAAGAGACCAACCATCAAAGAAGTTGTCGATTGGCTGGAAAATGTAGGGGCATCTCACCATGACTTGAATAAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1098

Amino Acids

119.41

Weight (kDa)

5.33

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 268 - 363 1.4e-06 Leucine-rich repeat region
LRR_14 PF23598 374 - 538 2.3e-07 Leucine-rich repeat region
LRR_8 PF13855 480 - 539 2.4e-09 Leucine rich repeat
LRR_8 PF13855 504 - 554 4.8e-08 Leucine rich repeat
LRR_14 PF23598 579 - 664 1.2e-06 Leucine-rich repeat region
Pkinase PF00069 815 - 1082 3.3e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 817 - 1083 8.7e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3166
Acc36I ACCTGC 2 cut(s) 1966, 3166
Acc65I GGTACC 1 cut(s) 2933
AccB1I GGYRCC 2 cut(s) 1022, 2933
AccI GTMKAC 3 cut(s) 537, 1419, 2484
AciI CCGC 6 cut(s) 297, 947, 1075, 1317, 1803, 2570
AclWI GGATC 5 cut(s) 204, 574, 1932, 2409, 3128
AcoI YGGCCR 2 cut(s) 2732, 2976
AcsI RAATTY 4 cut(s) 725, 1069, 1333, 2852
AcuI CTGAAG 4 cut(s) 512, 935, 2000, 2765
AdeI CACNNNGTG 1 cut(s) 2809
AfaI GTAC 4 cut(s) 1748, 2935, 2999, 3093
AfiI CCNNNNNNNGG 5 cut(s) 336, 341, 946, 2036, 2960
AflII CTTAAG 3 cut(s) 1306, 1505, 3218
AflIII ACRYGT 1 cut(s) 2863
AgeI ACCGGT 1 cut(s) 1547
AjnI CCWGG 8 cut(s) 334, 1275, 1455, 1490, 1651, 2080, 2272, 2361
AjuI GAANNNNNNNTTGG 2 cut(s) 2666, 2698
AleI CACNNNNGTG 2 cut(s) 885, 1364
AloI GAACNNNNNNTCC 2 cut(s) 2546, 2578
Alw21I GWGCWC 2 cut(s) 930, 1641
Alw26I GTCTC 4 cut(s) 178, 2532, 2900, 3217
AlwI GGATC 5 cut(s) 204, 574, 1932, 2409, 3128
AlwNI CAGNNNCTG 1 cut(s) 2768
Ama87I CYCGRG 2 cut(s) 2062, 2954
ApeKI GCWGC 8 cut(s) 142, 599, 1464, 1878, 1961, 2327, 3025, 3172
ApoI RAATTY 4 cut(s) 725, 1069, 1333, 2852
ArsI GACNNNNNNTTYG 2 cut(s) 3228, 3260
AseI ATTAAT 1 cut(s) 2654
AsiGI ACCGGT 1 cut(s) 1547
Asp718I GGTACC 1 cut(s) 2933
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 9 cut(s) 85, 299, 353, 734, 950, 1550, 1852, 2021, 2093
AsuC2I CCSGG 4 cut(s) 24, 782, 1886, 2887
AsuII TTCGAA 1 cut(s) 764
AsuNHI GCTAGC 2 cut(s) 994, 2236
AvaI CYCGRG 2 cut(s) 2062, 2954
AvaII GGWCC 5 cut(s) 85, 353, 1550, 1852, 2021
AxyI CCTNAGG 1 cut(s) 1632
BalI TGGCCA 2 cut(s) 2734, 2978
BanI GGYRCC 2 cut(s) 1022, 2933
BanII GRGCYC 2 cut(s) 930, 1641
BbsI GAAGAC 5 cut(s) 25, 36, 419, 769, 1980
Bbv12I GWGCWC 2 cut(s) 930, 1641
BbvCI CCTCAGC 1 cut(s) 3120
BbvI GCAGC 8 cut(s) 129, 586, 1451, 1890, 1948, 2314, 3012, 3159
BceAI ACGGC 3 cut(s) 1882, 2756, 3028
BciT130I CCWGG 8 cut(s) 336, 1277, 1457, 1492, 1653, 2082, 2274, 2363
BciVI GTATCC 2 cut(s) 2296, 2347
BcnI CCSGG 4 cut(s) 24, 782, 1886, 2887
BcoDI GTCTC 4 cut(s) 178, 2532, 2900, 3217
BfmI CTRYAG 2 cut(s) 600, 3173
BfoI RGCGCY 1 cut(s) 23
BfrI CTTAAG 3 cut(s) 1306, 1505, 3218
BfuAI ACCTGC 2 cut(s) 1966, 3166
BfuI GTATCC 2 cut(s) 2296, 2347
BglI GCCNNNNNGGC 3 cut(s) 1322, 1803, 2740
Bme18I GGWCC 5 cut(s) 85, 353, 1550, 1852, 2021
BmeT110I CYCGRG 2 cut(s) 2062, 2954
BmgT120I GGNCC 9 cut(s) 85, 299, 353, 734, 950, 1550, 1852, 2021, 2093
BmrI ACTGGG 1 cut(s) 1554
BmtI GCTAGC 2 cut(s) 998, 2240
BmuI ACTGGG 1 cut(s) 1554
BoxI GACNNNNGTC 1 cut(s) 1270
BpiI GAAGAC 5 cut(s) 25, 36, 419, 769, 1980
BplI GAGNNNNNCTC 2 cut(s) 917, 949
BpmI CTGGAG 4 cut(s) 944, 1641, 1755, 2055
Bpu10I CCTNAGC 3 cut(s) 1799, 2340, 3120
Bpu14I TTCGAA 1 cut(s) 764
BpuEI CTTGAG 3 cut(s) 490, 2366, 3041
BpuMI CCSGG 4 cut(s) 24, 782, 1886, 2887
BsaBI GATNNNNATC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 3217
BsaJI CCNNGG 7 cut(s) 335, 1147, 1275, 1455, 1456, 2938, 3062
BsaWI WCCGGW 2 cut(s) 746, 1547
BsaXI ACNNNNNCTCC 4 cut(s) 2119, 2149, 3066, 3096
Bsc4I CCNNNNNNNGG 5 cut(s) 336, 341, 946, 2036, 2960
Bse118I RCCGGY 2 cut(s) 1230, 1547
Bse1I ACTGG 6 cut(s) 1171, 1560, 1624, 1709, 2150, 3037
Bse21I CCTNAGG 1 cut(s) 1632
Bse3DI GCAATG 3 cut(s) 1318, 2226, 2238
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 8 cut(s) 336, 1277, 1457, 1492, 1653, 2082, 2274, 2363
BseDI CCNNGG 7 cut(s) 335, 1147, 1275, 1455, 1456, 2938, 3062
BseGI GGATG 6 cut(s) 590, 1408, 1653, 1881, 2835, 2851
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 5 cut(s) 336, 341, 946, 2036, 2960
BseMI GCAATG 3 cut(s) 1318, 2226, 2238
BseMII CTCAG 7 cut(s) 30, 654, 2111, 2331, 2547, 3111, 3134
BseNI ACTGG 6 cut(s) 1171, 1560, 1624, 1709, 2150, 3037
BseRI GAGGAG 2 cut(s) 798, 3003
BseXI GCAGC 8 cut(s) 129, 586, 1451, 1890, 1948, 2314, 3012, 3159
BseYI CCCAGC 1 cut(s) 1319
BsgI GTGCAG 2 cut(s) 1033, 2088
BshNI GGYRCC 2 cut(s) 1022, 2933
BshTI ACCGGT 1 cut(s) 1547
BsiHKAI GWGCWC 2 cut(s) 930, 1641
BsiHKCI CYCGRG 2 cut(s) 2062, 2954
BsiSI CCGG 8 cut(s) 24, 747, 781, 1231, 1400, 1548, 1886, 2887
BslFI GGGAC 4 cut(s) 71, 1258, 2034, 2869
BslI CCNNNNNNNGG 5 cut(s) 336, 341, 946, 2036, 2960
BsmAI GTCTC 4 cut(s) 178, 2532, 2900, 3217
BsmFI GGGAC 4 cut(s) 71, 1258, 2034, 2869
Bso31I GGTCTC 1 cut(s) 3217
BsoBI CYCGRG 2 cut(s) 2062, 2954
Bsp119I TTCGAA 1 cut(s) 764
Bsp1286I GDGCHC 2 cut(s) 930, 1641
Bsp1407I TGTACA 1 cut(s) 2997
BspACI CCGC 6 cut(s) 297, 947, 1075, 1317, 1803, 2570
BspCNI CTCAG 7 cut(s) 29, 655, 2110, 2332, 2546, 3112, 3135
BspMAI CTGCAG 2 cut(s) 604, 3177
BspMI ACCTGC 2 cut(s) 1966, 3166
BspOI GCTAGC 2 cut(s) 998, 2240
BspPI GGATC 5 cut(s) 204, 574, 1932, 2409, 3128
BspT104I TTCGAA 1 cut(s) 764
BspT107I GGYRCC 2 cut(s) 1022, 2933
BspTI CTTAAG 3 cut(s) 1306, 1505, 3218
BspTNI GGTCTC 1 cut(s) 3217
BsrDI GCAATG 3 cut(s) 1318, 2226, 2238
BsrFI RCCGGY 2 cut(s) 1230, 1547
BsrGI TGTACA 1 cut(s) 2997
BsrI ACTGG 6 cut(s) 1171, 1560, 1624, 1709, 2150, 3037
BssAI RCCGGY 2 cut(s) 1230, 1547
BssECI CCNNGG 7 cut(s) 335, 1147, 1275, 1455, 1456, 2938, 3062
BssNAI GTATAC 1 cut(s) 2485
BssT1I CCWWGG 3 cut(s) 1147, 2938, 3062
Bst1107I GTATAC 1 cut(s) 2485
Bst2UI CCWGG 8 cut(s) 336, 1277, 1457, 1492, 1653, 2082, 2274, 2363
Bst4CI ACNGT 1 cut(s) 3002
Bst6I CTCTTC 1 cut(s) 3159
BstAFI CTTAAG 3 cut(s) 1306, 1505, 3218
BstAPI GCANNNNNTGC 1 cut(s) 2867
BstAUI TGTACA 1 cut(s) 2997
BstBI TTCGAA 1 cut(s) 764
BstEII GGTNACC 1 cut(s) 971
BstF5I GGATG 6 cut(s) 590, 1408, 1653, 1881, 2835, 2851
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 1 cut(s) 22
BstMAI GTCTC 4 cut(s) 178, 2532, 2900, 3217
BstNI CCWGG 8 cut(s) 336, 1277, 1457, 1492, 1653, 2082, 2274, 2363
BstNSI RCATGY 3 cut(s) 2785, 2867, 3199
BstPAI GACNNNNGTC 1 cut(s) 1270
BstPI GGTNACC 1 cut(s) 971
BstSFI CTRYAG 2 cut(s) 600, 3173
BstV1I GCAGC 8 cut(s) 129, 586, 1451, 1890, 1948, 2314, 3012, 3159
BstV2I GAAGAC 5 cut(s) 25, 36, 419, 769, 1980
BstX2I RGATCY 2 cut(s) 1924, 2401
BstYI RGATCY 2 cut(s) 1924, 2401
BstZ17I GTATAC 1 cut(s) 2485
Bsu36I CCTNAGG 1 cut(s) 1632
BsuI GTATCC 2 cut(s) 2296, 2347
BtsCI GGATG 6 cut(s) 590, 1408, 1653, 1881, 2835, 2851
BtsI GCAGTG 3 cut(s) 2322, 2589, 2615
BtsIMutI CAGTG 6 cut(s) 736, 1164, 2322, 2589, 2615, 3030
BveI ACCTGC 2 cut(s) 1966, 3166
CaiI CAGNNNCTG 1 cut(s) 2768
CfoI GCGC 1 cut(s) 22
Cfr10I RCCGGY 2 cut(s) 1230, 1547
Cfr13I GGNCC 9 cut(s) 85, 299, 353, 734, 950, 1550, 1852, 2021, 2093
Csp6I GTAC 4 cut(s) 1747, 2934, 2998, 3092
CspAI ACCGGT 1 cut(s) 1547
CspCI CAANNNNNGTGG 2 cut(s) 712, 747
CviQI GTAC 4 cut(s) 1747, 2934, 2998, 3092
DraIII CACNNNGTG 1 cut(s) 2809
EaeI YGGCCR 2 cut(s) 2732, 2976
Eam1104I CTCTTC 1 cut(s) 3159
EarI CTCTTC 1 cut(s) 3159
EciI GGCGGA 1 cut(s) 2585
Ecl136II GAGCTC 2 cut(s) 928, 1639
Eco130I CCWWGG 3 cut(s) 1147, 2938, 3062
Eco24I GRGCYC 2 cut(s) 930, 1641
Eco31I GGTCTC 1 cut(s) 3217
Eco32I GATATC 2 cut(s) 1248, 2821
Eco47I GGWCC 5 cut(s) 85, 353, 1550, 1852, 2021
Eco53kI GAGCTC 2 cut(s) 928, 1639
Eco57I CTGAAG 4 cut(s) 512, 935, 2000, 2765
Eco81I CCTNAGG 1 cut(s) 1632
Eco88I CYCGRG 2 cut(s) 2062, 2954
Eco91I GGTNACC 1 cut(s) 971
EcoICRI GAGCTC 2 cut(s) 928, 1639
EcoO109I RGGNCCY 2 cut(s) 85, 2021
EcoO65I GGTNACC 1 cut(s) 971
EcoRII CCWGG 8 cut(s) 334, 1275, 1455, 1490, 1651, 2080, 2272, 2361
EcoRV GATATC 2 cut(s) 1248, 2821
EcoT14I CCWWGG 3 cut(s) 1147, 2938, 3062
EcoT22I ATGCAT 1 cut(s) 2787
EcoT38I GRGCYC 2 cut(s) 930, 1641
ErhI CCWWGG 3 cut(s) 1147, 2938, 3062
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FaqI GGGAC 4 cut(s) 71, 1258, 2034, 2869
FauI CCCGC 2 cut(s) 940, 1068
FauNDI CATATG 1 cut(s) 2902
FblI GTMKAC 3 cut(s) 537, 1419, 2484
FokI GGATG 6 cut(s) 577, 1415, 1660, 1868, 2822, 2858
FriOI GRGCYC 2 cut(s) 930, 1641
GlaI GCGC 1 cut(s) 21
GsaI CCCAGC 1 cut(s) 1323
GsuI CTGGAG 4 cut(s) 944, 1641, 1755, 2055
HaeII RGCGCY 1 cut(s) 23
HapII CCGG 8 cut(s) 24, 747, 781, 1231, 1400, 1548, 1886, 2887
HhaI GCGC 1 cut(s) 22
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HincII GTYRAC 2 cut(s) 1537, 3205
HindII GTYRAC 2 cut(s) 1537, 3205
HindIII AAGCTT 3 cut(s) 524, 1502, 2680
HpaI GTTAAC 1 cut(s) 1537
HpaII CCGG 8 cut(s) 24, 747, 781, 1231, 1400, 1548, 1886, 2887
Hpy166II GTNNAC 6 cut(s) 538, 1265, 1420, 1537, 2485, 3205
Hpy8I GTNNAC 6 cut(s) 538, 1265, 1420, 1537, 2485, 3205
Hpy99I CGWCG 1 cut(s) 599
HpyCH4III ACNGT 1 cut(s) 3002
HspAI GCGC 1 cut(s) 20
KflI GGGWCCC 1 cut(s) 2021
KpnI GGTACC 1 cut(s) 2937
KspAI GTTAAC 1 cut(s) 1537
LmnI GCTCC 8 cut(s) 76, 220, 653, 774, 811, 925, 1636, 2759
Lsp1109I GCAGC 8 cut(s) 129, 586, 1451, 1890, 1948, 2314, 3012, 3159
MaeIII GTNAC 9 cut(s) 5, 185, 971, 1173, 1200, 2332, 2694, 2914, 2942
MfeI CAATTG 1 cut(s) 941
MflI RGATCY 2 cut(s) 1924, 2401
MhlI GDGCHC 2 cut(s) 930, 1641
MlsI TGGCCA 2 cut(s) 2734, 2978
MluNI TGGCCA 2 cut(s) 2734, 2978
MlyI GAGTC 5 cut(s) 8, 744, 1280, 1415, 2902
MmeI TCCRAC 5 cut(s) 868, 966, 1150, 1830, 2141
Mox20I TGGCCA 2 cut(s) 2734, 2978
Mph1103I ATGCAT 1 cut(s) 2787
MscI TGGCCA 2 cut(s) 2734, 2978
MslI CAYNNNNRTG 5 cut(s) 885, 1364, 1844, 2231, 2640
Msp20I TGGCCA 2 cut(s) 2734, 2978
MspA1I CMGCKG 3 cut(s) 1253, 1319, 1756
MspCI CTTAAG 3 cut(s) 1306, 1505, 3218
MspI CCGG 8 cut(s) 24, 747, 781, 1231, 1400, 1548, 1886, 2887
MunI CAATTG 1 cut(s) 941
MvaI CCWGG 8 cut(s) 336, 1277, 1457, 1492, 1653, 2082, 2274, 2363
NciI CCSGG 4 cut(s) 24, 782, 1886, 2887
NdeI CATATG 1 cut(s) 2902
NheI GCTAGC 2 cut(s) 994, 2236
NmuCI GTSAC 2 cut(s) 5, 1200
NsiI ATGCAT 1 cut(s) 2787
NspI RCATGY 3 cut(s) 2785, 2867, 3199
NspV TTCGAA 1 cut(s) 764
OliI CACNNNNGTG 2 cut(s) 885, 1364
PaeR7I CTCGAG 1 cut(s) 2062
PaqCI CACCTGC 1 cut(s) 3166
PasI CCCWGGG 1 cut(s) 1456
PciI ACATGT 1 cut(s) 2863
PfeI GAWTC 6 cut(s) 1218, 1351, 2077, 2175, 2282, 2893
PfoI TCCNGGA 2 cut(s) 2080, 2272
PinAI ACCGGT 1 cut(s) 1547
PleI GAGTC 5 cut(s) 8, 744, 1279, 1415, 2902
PpsI GAGTC 5 cut(s) 8, 744, 1279, 1415, 2902
PpuMI RGGWCCY 2 cut(s) 85, 2021
PscI ACATGT 1 cut(s) 2863
PshAI GACNNNNGTC 1 cut(s) 1270
PshBI ATTAAT 1 cut(s) 2654
Psp124BI GAGCTC 2 cut(s) 930, 1641
Psp5II RGGWCCY 2 cut(s) 85, 2021
Psp6I CCWGG 8 cut(s) 334, 1275, 1455, 1490, 1651, 2080, 2272, 2361
PspEI GGTNACC 1 cut(s) 971
PspFI CCCAGC 1 cut(s) 1319
PspGI CCWGG 8 cut(s) 334, 1275, 1455, 1490, 1651, 2080, 2272, 2361
PspPI GGNCC 9 cut(s) 85, 299, 353, 734, 950, 1550, 1852, 2021, 2093
PspPPI RGGWCCY 2 cut(s) 85, 2021
PspXI VCTCGAGB 1 cut(s) 2062
PstI CTGCAG 2 cut(s) 604, 3177
PstNI CAGNNNCTG 1 cut(s) 2768
PsuI RGATCY 2 cut(s) 1924, 2401
PvuII CAGCTG 2 cut(s) 1253, 1756
RsaI GTAC 4 cut(s) 1748, 2935, 2999, 3093
RsaNI GTAC 4 cut(s) 1747, 2934, 2998, 3092
RseI CAYNNNNRTG 5 cut(s) 885, 1364, 1844, 2231, 2640
SacI GAGCTC 2 cut(s) 930, 1641
Sau96I GGNCC 9 cut(s) 85, 299, 353, 734, 950, 1550, 1852, 2021, 2093
SchI GAGTC 5 cut(s) 8, 744, 1280, 1415, 2902
SduI GDGCHC 2 cut(s) 930, 1641
SfcI CTRYAG 2 cut(s) 600, 3173
Sfr274I CTCGAG 1 cut(s) 2062
SfuI TTCGAA 1 cut(s) 764
SinI GGWCC 5 cut(s) 85, 353, 1550, 1852, 2021
SlaI CTCGAG 1 cut(s) 2062
SmiMI CAYNNNNRTG 5 cut(s) 885, 1364, 1844, 2231, 2640
SmlI CTYRAG 7 cut(s) 469, 1306, 1505, 2062, 2345, 3020, 3218
SmoI CTYRAG 7 cut(s) 469, 1306, 1505, 2062, 2345, 3020, 3218
SsiI CCGC 6 cut(s) 297, 947, 1075, 1317, 1803, 2570
SstI GAGCTC 2 cut(s) 930, 1641
StyI CCWWGG 3 cut(s) 1147, 2938, 3062
TaaI ACNGT 1 cut(s) 3002
TaqI TCGA 8 cut(s) 313, 578, 764, 828, 935, 1143, 2063, 3246
TatI WGTACW 2 cut(s) 1746, 2997
TauI GCSGC 3 cut(s) 300, 1319, 1806
TfiI GAWTC 6 cut(s) 1218, 1351, 2077, 2175, 2282, 2893
TscAI CASTG 6 cut(s) 736, 1171, 2329, 2596, 2622, 3037
TseFI GTSAC 2 cut(s) 5, 1200
TseI GCWGC 8 cut(s) 142, 599, 1464, 1878, 1961, 2327, 3025, 3172
Tsp45I GTSAC 2 cut(s) 5, 1200
TspGWI ACGGA 1 cut(s) 583
TspRI CASTG 6 cut(s) 736, 1171, 2329, 2596, 2622, 3037
Vha464I CTTAAG 3 cut(s) 1306, 1505, 3218
VpaK11BI GGWCC 5 cut(s) 85, 353, 1550, 1852, 2021
VspI ATTAAT 1 cut(s) 2654
XapI RAATTY 4 cut(s) 725, 1069, 1333, 2852
XbaI TCTAGA 1 cut(s) 1699
XceI RCATGY 3 cut(s) 2785, 2867, 3199
XcmI CCANNNNNNNNNTGG 1 cut(s) 1560
XhoI CTCGAG 1 cut(s) 2062
XmiI GTMKAC 3 cut(s) 537, 1419, 2484
Zsp2I ATGCAT 1 cut(s) 2787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.