Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008419

Physical Location & Seq

Genomic Coordinates Reverse (-)

7126279 .. 7130729

Gene Structure 4451 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G16440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2544 bp

ATGGCTTCTGATTCTTCTCTCGGCAACACCAACGACGGTCCTCCTTCTCCCGGAGAAAACGTTTCAAGCCCTATCGAAAACACATACTCTTCCCCCGCCGCACTTCACCGTCGCCGGCGAGGAAGATCCTCTACTCCCACGCAATTCGCAACTCCTCCACCACCGCCTTCACGTCTCGCATCATCCAATTCCACTCCTCCAACATCTCGTCCTTCCGCCGCCAGATCTAAAGGAAGAAATGGACACGGAGGTGGCGGTGGTGGCGGTGGTGGCGGAGACCCAGGGACGCCAATGTCCACTGACGAGCCACTGCCTTCTTCCGATGACGGTGAAGAAGACGGAGGCGACGATACTACTCCGACTTTCGTTTGGGGAACTAACATCAGTGTGCAAGATGTTAAATCTGCGATTGAAATGTTTGTTAAGCATTTCAGAGAAGCTAGAGAGAATAGTGATGATTTGTTTAGAGAAGGGAAGTATATGGTTTCGATAAGGAAGGTGATTGAGATTGAAGGAGAATGGATTGATGTTGATGCGTTTGATGTGTTTGATTATGATCCTGATTTGTATAACAAGATGGTTCGTTATCCTTTGGAAGTTCTCGCTATCTTCGATATTGTGCTGATGGATATTGTTTCCACGATTAATCGTTTGTTCGAGAAACATGTTCAAGTTAGGATTTTTAACCTCAGGACATCTACTTCCATGAGAAATCTCAATCCATCTGATATCGAGAAGATGATCTCTTTGAAAGGTATGATAATTCGGAGTAGCTCTATCATTCCTGAAATAAGGGAAGCTGTGTTTAGATGCCTTGTTTGTGGTTACTTCTCTGACCCCATCATCGTTGATAGAGGGAAAATAAGTGAGCCTCCTACTTGCTTGAAACAAGAGTGCATGACCAAGAACTCCATGACACTAGTGCACAACCGATGCAGGTTTGCTGATAAGCAGATTGTGAGGCTTCAGGAAACACCTGATGAGATTCCAGAAGGAGGAACACCTCACACAGTTAGCTTGTTGCTGCATGATAAGCTAGTTGATAATGGGAAGCCTGGTGATAGAATTGAGGTCACCGGAATTTACAGAGCAATGACTGTCCGAGTGGGACCTGCTCACAGGACTGTGAAATCTGTGTTTAAGACCTATATCGATTGCCTTCACATAAAGAAAGCAAGTAAGTTAAGAATGTCTGCTGAGGATCCTATGGATGTTGACAATAGTCTGCGTAGAGTTGATGAAGATGTCGAGTTAGATGAGGAGAAGCTGAGGAAGTTTCAGGAACTCTCTAAACAACCAGATATATATGAGAGGCTTAGTAGATCACTAGCACCAAACATCTGGGAGTTGGATGATGTTAAAAAGGGTCTTCTATGCCAGCTTTTTGGAGGGAATGCTTTGAACTTAGCATCTGGTGCTAATTTTCGTGGTGACATCAACATCTTACTTGTTGGTGACCCTGGTACGAGCAAGTCCCAGCTGCTCCAGTACATTCATAAGCTTTCACCCCGTGGCATTTACACAAGTGGGCGAGGGAGCTCAGCTGTTGGTTTAACAGCTTATGTAGCTAAAGATCCTGAGACAGGAGAAACTGTTTTGGAGAGTGGAGCTCTTGTTCTTAGTGACCGAGGTATCTGCTGTATTGATGAATTTGACAAAATGTCTGACAGTGCGAGGAGCATGCTACACGAGGTGATGGAACAGCAGACTGTTTCAATAGCAAAGGCTGGTATTATTGCATCTCTAAATGCCAGAACCTCTGTGTTGGCTTGTGCAAATCCTAGTGGCTCACGTTATAATCCCCGGCTTTCTGTTATTGAGAATATTCACCTTCCTCCAACCTTGCTATCTAGATTCGATTTGATCTACTTGATTCTTGACAAGCCCGATGAGCAGACTGACCGAAGGCTTGCAAAGCATATTGTGGCCCTTCACTTTGAGAACGCTGAGAGTGCTCAGGAGGAAGCTATAGATATTACTACACTGACAACTTATGTTAGCTATGCCCGCAAGAACATTCATCCTAAACTGTCAGATGAAGCTGCAGAGGAGTTGACCCGAGGATATGTTGAGCTGAGAAAAGCAGGGAAGTTTGCTGGCAGTAGCAAAAAGGTCATAACAGCAACTCCTAGGCAGATTGAAAGTTTGATCAGACTTAGTGAGGCCTTGGCTCGGATGCGCTTCTCCGAATGGGTTGAAAAGCATGACGTGGACGAGGCATTTCGACTTCTCAGAGTTGCAATGCAGCAATCAGCAACGGATCACGCCACAGGGACCATTGACATGGACCTGATAAATACTGGAGTGTCAGCAAGTGAACGAATGAGGCGGGATACCTTTGCGTCGTCAATTAGAGACATAGCTCTGGAGAAGATGCAAATTGGAGGATCATCAATGCGCTTATCCGAGTTACTTGAAGAACTGAAGAAGCATGGAGGCAACATAAACACCGAAATTCATCTTCATGATGTTAGAAAAGCAGTCGCGACGCTAGCGAGTGAAGGATTTCTGGTAGCTGAAGGTGATAGAATCAAGAGAGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0000785 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

847

Amino Acids

93.66

Weight (kDa)

5.94

Isoelectric Point (pI)

47.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	136 - 229	8.6e-11	MCM N-terminal domain
MCM_OB	PF17207	236 - 365	6.1e-39	MCM OB domain
MCM	PF00493	423 - 645	8.8e-101	MCM P-loop domain
AAA_5	PF07728	480 - 596	1.1e-06	AAA domain (dynein-related subfamily)
MCM_lid	PF17855	661 - 747	3.8e-28	MCM AAA-lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008419)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16440
fragaria_vesca	FvH4_2g09990
malus_domestica	MD00G1085600.v1.1 MD10G1066500.v1.1
prunus_persica	Prupe.8G088400_v2.0.a1
pyrus_communis	pycom05g04970 pycom05g04980 pycom10g05200
rosa_chinensis	RchiOBHm_Chr6g0266881 RchiOBHm_Chr6g0266891
rosa_laevigata	RLG00000014063 RLG00000014064 RLG00000014065
rosa_multiflora	Rmu_co8507775.1_g000001 Rmu_sc0021142.1_g000001 Rmu_sc0036177.1_g000001
rosa_roxburghii	Rroxscaffold_7G00201330
rosa_rugosa	Rorug06G0027900
rosa_samantha	Rh6AG149000 Rh6BG150000 Rh6CG144900 Rh6DG134200
rosa_wichuraiana	Rw6G012890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1797
AasI	GACNNNNNNGTC	1 cut(s)	292
Acc36I	ACCTGC	2 cut(s)	927, 1120
AccII	CGCG	1 cut(s)	2486
AclI	AACGTT	1 cut(s)	60
AclWI	GGATC	7 cut(s)	120, 551, 1196, 1209, 1568, 2268, 2395
AcsI	RAATTY	3 cut(s)	1080, 1649, 2454
AcuI	CTGAAG	3 cut(s)	950, 2444, 2538
AcyI	GRCGYC	1 cut(s)	287
AdeI	CACNNNGTG	2 cut(s)	1511, 1693
AfaI	GTAC	2 cut(s)	1465, 1490
AfiI	CCNNNNNNNGG	4 cut(s)	50, 995, 1583, 2172
AflIII	ACRYGT	1 cut(s)	664
AhdI	GACNNNNNGTC	1 cut(s)	1660
AhlI	ACTAGT	1 cut(s)	919
AjiI	CACGTC	2 cut(s)	173, 2209
AjnI	CCWGG	3 cut(s)	280, 1054, 1459
AleI	CACNNNNGTG	1 cut(s)	249
AloI	GAACNNNNNNTCC	2 cut(s)	1599, 1631
Alw21I	GWGCWC	4 cut(s)	927, 1541, 1612, 1957
Alw26I	GTCTC	4 cut(s)	179, 270, 1574, 2349
Alw44I	GTGCAC	1 cut(s)	923
AlwI	GGATC	7 cut(s)	120, 551, 1196, 1209, 1568, 2268, 2395
Ama87I	CYCGRG	1 cut(s)	2058
AoxI	GGCC	2 cut(s)	1926, 2163
ApaLI	GTGCAC	1 cut(s)	923
ApeKI	GCWGC	4 cut(s)	1024, 1480, 2042, 2245
ApoI	RAATTY	3 cut(s)	1080, 1649, 2454
AseI	ATTAAT	1 cut(s)	645
Asp700I	GAANNNNTTC	1 cut(s)	2505
AspA2I	CCTAGG	1 cut(s)	2129
AspLEI	GCGC	2 cut(s)	2181, 2400
AspS9I	GGNCC	5 cut(s)	38, 1109, 1927, 2274, 2287
AsuC2I	CCSGG	2 cut(s)	51, 1804
AsuNHI	GCTAGC	1 cut(s)	2491
AvaI	CYCGRG	1 cut(s)	2058
AvaII	GGWCC	4 cut(s)	38, 1109, 2274, 2287
AvrII	CCTAGG	1 cut(s)	2129
AxyI	CCTNAGG	1 cut(s)	689
BaeGI	GKGCMC	1 cut(s)	927
BaeI	ACNNNNGTAYC	2 cut(s)	1615, 1648
BamHI	GGATCC	1 cut(s)	1201
BanII	GRGCYC	2 cut(s)	1541, 1612
BarI	GAAGNNNNNNTAC	2 cut(s)	115, 147
BauI	CACGAG	1 cut(s)	1688
BbsI	GAAGAC	2 cut(s)	342, 1361
Bbv12I	GWGCWC	4 cut(s)	927, 1541, 1612, 1957
BbvCI	CCTCAGC	2 cut(s)	1197, 1268
BbvI	GCAGC	4 cut(s)	1011, 1467, 2029, 2257
BccI	CCATC	5 cut(s)	571, 619, 730, 848, 1690
BcgI	CGANNNNNNTGC	4 cut(s)	1617, 1651, 1663, 1697
BciT130I	CCWGG	3 cut(s)	282, 1056, 1461
BciVI	GTATCC	1 cut(s)	2326
BclI	TGATCA	1 cut(s)	2148
BcnI	CCSGG	2 cut(s)	51, 1804
BcoDI	GTCTC	4 cut(s)	179, 270, 1574, 2349
BcuI	ACTAGT	1 cut(s)	919
BfaI	CTAG	8 cut(s)	441, 920, 1037, 1328, 1782, 1851, 2130, 2492
BfmI	CTRYAG	2 cut(s)	1968, 2043
BfuAI	ACCTGC	2 cut(s)	927, 1120
BfuI	GTATCC	1 cut(s)	2326
BglII	AGATCT	1 cut(s)	224
BisI	GCNGC	6 cut(s)	99, 219, 1025, 1481, 2043, 2246
BlnI	CCTAGG	1 cut(s)	2129
BlpI	GCTNAGC	1 cut(s)	1540
BlsI	GCNGC	6 cut(s)	100, 220, 1026, 1482, 2044, 2247
Bme1390I	CCNGG	5 cut(s)	51, 282, 1056, 1461, 1804
Bme18I	GGWCC	4 cut(s)	38, 1109, 2274, 2287
BmeRI	GACNNNNNGTC	1 cut(s)	1660
BmeT110I	CYCGRG	1 cut(s)	2058
BmgBI	CACGTC	2 cut(s)	173, 2209
BmgT120I	GGNCC	5 cut(s)	38, 1109, 1927, 2274, 2287
BmiI	GGNNCC	3 cut(s)	1110, 1203, 2275
BmrFI	CCNGG	5 cut(s)	51, 282, 1056, 1461, 1804
BmsI	GCATC	8 cut(s)	188, 523, 800, 923, 1418, 1748, 2166, 2364
BmtI	GCTAGC	1 cut(s)	2495
BoxI	GACNNNNGTC	1 cut(s)	1221
BpiI	GAAGAC	2 cut(s)	342, 1361
BplI	GAGNNNNNCTC	2 cut(s)	1594, 1626
BpmI	CTGGAG	3 cut(s)	1469, 2322, 2387
Bpu10I	CCTNAGC	3 cut(s)	1197, 1268, 1956
Bpu1102I	GCTNAGC	1 cut(s)	1540
BpuMI	CCSGG	2 cut(s)	51, 1804
Bsa29I	ATCGAT	1 cut(s)	1152
BsaBI	GATNNNNATC	1 cut(s)	555
BsaHI	GRCGYC	1 cut(s)	287
BsaI	GGTCTC	1 cut(s)	270
BsaJI	CCNNGG	9 cut(s)	280, 281, 1459, 1510, 1627, 1802, 2059, 2129, 2166
BsaWI	WCCGGW	1 cut(s)	1076
BsaXI	ACNNNNNCTCC	8 cut(s)	45, 75, 1578, 1599, 1608, 1629, 2110, 2140
Bsc4I	CCNNNNNNNGG	4 cut(s)	50, 995, 1583, 2172
Bse118I	RCCGGY	1 cut(s)	114
Bse1I	ACTGG	2 cut(s)	1486, 2305
Bse21I	CCTNAGG	1 cut(s)	689
Bse3DI	GCAATG	2 cut(s)	1098, 2247
Bse8I	GATNNNNATC	1 cut(s)	555
BseBI	CCWGG	3 cut(s)	282, 1056, 1461
BseCI	ATCGAT	1 cut(s)	1152
BseDI	CCNNGG	9 cut(s)	280, 281, 1459, 1510, 1627, 1802, 2059, 2129, 2166
BseGI	GGATG	5 cut(s)	182, 1216, 1357, 2020, 2181
BseJI	GATNNNNATC	1 cut(s)	555
BseLI	CCNNNNNNNGG	4 cut(s)	50, 995, 1583, 2172
BseMI	GCAATG	2 cut(s)	1098, 2247
BseMII	CTCAG	9 cut(s)	703, 1188, 1259, 1554, 1569, 1938, 1970, 2066, 2245
BseNI	ACTGG	2 cut(s)	1486, 2305
BseRI	GAGGAG	5 cut(s)	144, 186, 1274, 1690, 2063
BseSI	GKGCMC	1 cut(s)	927
BseXI	GCAGC	4 cut(s)	1011, 1467, 2029, 2257
BseYI	CCCAGC	1 cut(s)	1476
Bsh1236I	CGCG	1 cut(s)	2486
BshFI	GGCC	2 cut(s)	1928, 2165
BshVI	ATCGAT	1 cut(s)	1152
BsiHKAI	GWGCWC	4 cut(s)	927, 1541, 1612, 1957
BsiHKCI	CYCGRG	1 cut(s)	2058
BsiSI	CCGG	4 cut(s)	51, 115, 1077, 1804
BslFI	GGGAC	4 cut(s)	298, 1122, 1459, 2287
BslI	CCNNNNNNNGG	4 cut(s)	50, 995, 1583, 2172
BsmAI	GTCTC	4 cut(s)	179, 270, 1574, 2349
BsmBI	CGTCTC	1 cut(s)	179
BsmFI	GGGAC	4 cut(s)	298, 1122, 1459, 2287
BsmI	GAATGC	1 cut(s)	1399
BsnI	GGCC	2 cut(s)	1928, 2165
Bso31I	GGTCTC	1 cut(s)	270
BsoBI	CYCGRG	1 cut(s)	2058
Bsp1286I	GDGCHC	4 cut(s)	927, 1541, 1612, 1957
Bsp1720I	GCTNAGC	1 cut(s)	1540
Bsp68I	TCGCGA	1 cut(s)	2486
BspANI	GGCC	2 cut(s)	1928, 2165
BspCNI	CTCAG	9 cut(s)	702, 1189, 1260, 1553, 1570, 1939, 1969, 2067, 2244
BspDI	ATCGAT	1 cut(s)	1152
BspFNI	CGCG	1 cut(s)	2486
BspHI	TCATGA	1 cut(s)	2464
BspLI	GGNNCC	3 cut(s)	1110, 1203, 2275
BspMAI	CTGCAG	1 cut(s)	2047
BspMI	ACCTGC	2 cut(s)	927, 1120
BspOI	GCTAGC	1 cut(s)	2495
BspPI	GGATC	7 cut(s)	120, 551, 1196, 1209, 1568, 2268, 2395
BspTNI	GGTCTC	1 cut(s)	270
BsrDI	GCAATG	2 cut(s)	1098, 2247
BsrFI	RCCGGY	1 cut(s)	114
BsrI	ACTGG	2 cut(s)	1486, 2305
BssAI	RCCGGY	1 cut(s)	114
BssECI	CCNNGG	9 cut(s)	280, 281, 1459, 1510, 1627, 1802, 2059, 2129, 2166
BssNI	GRCGYC	1 cut(s)	287
BssSI	CACGAG	1 cut(s)	1688
BssT1I	CCWWGG	2 cut(s)	2129, 2166
Bst2BI	CACGAG	1 cut(s)	1688
Bst2UI	CCWGG	3 cut(s)	282, 1056, 1461
Bst6I	CTCTTC	1 cut(s)	94
BstACI	GRCGYC	1 cut(s)	287
BstAPI	GCANNNNNTGC	1 cut(s)	1415
BstC8I	GCNNGC	7 cut(s)	116, 1379, 1682, 1911, 2008, 2098, 2493
BstDSI	CCRYGG	1 cut(s)	1510
BstEII	GGTNACC	2 cut(s)	1072, 1454
BstENI	CCTNNNNNAGG	1 cut(s)	1581
BstF5I	GGATG	5 cut(s)	182, 1216, 1357, 2020, 2181
BstFNI	CGCG	1 cut(s)	2486
BstHHI	GCGC	2 cut(s)	2181, 2400
BstMAI	GTCTC	4 cut(s)	179, 270, 1574, 2349
BstNI	CCWGG	3 cut(s)	282, 1056, 1461
BstNSI	RCATGY	2 cut(s)	668, 1684
BstPAI	GACNNNNGTC	1 cut(s)	1221
BstPI	GGTNACC	2 cut(s)	1072, 1454
BstSCI	CCNGG	5 cut(s)	49, 280, 1054, 1459, 1802
BstSFI	CTRYAG	2 cut(s)	1968, 2043
BstSLI	GKGCMC	1 cut(s)	927
BstUI	CGCG	1 cut(s)	2486
BstV1I	GCAGC	4 cut(s)	1011, 1467, 2029, 2257
BstV2I	GAAGAC	2 cut(s)	342, 1361
BstX2I	RGATCY	4 cut(s)	125, 224, 1201, 1573
BstXI	CCANNNNNNTGG	3 cut(s)	1341, 1385, 2284
BstYI	RGATCY	4 cut(s)	125, 224, 1201, 1573
Bsu15I	ATCGAT	1 cut(s)	1152
Bsu36I	CCTNAGG	1 cut(s)	689
BsuI	GTATCC	1 cut(s)	2326
BsuRI	GGCC	2 cut(s)	1928, 2165
BsuTUI	ATCGAT	1 cut(s)	1152
BtgI	CCRYGG	1 cut(s)	1510
BtrI	CACGTC	2 cut(s)	173, 2209
BtsCI	GGATG	5 cut(s)	182, 1216, 1357, 2020, 2181
BtsI	GCAGTG	1 cut(s)	308
BtsIMutI	CAGTG	5 cut(s)	297, 308, 391, 1675, 1982
BtuMI	TCGCGA	1 cut(s)	2486
BveI	ACCTGC	2 cut(s)	927, 1120
Cac8I	GCNNGC	7 cut(s)	116, 1379, 1682, 1911, 2008, 2098, 2493
CciI	TCATGA	1 cut(s)	2464
CfoI	GCGC	2 cut(s)	2181, 2400
Cfr10I	RCCGGY	1 cut(s)	114
Cfr13I	GGNCC	5 cut(s)	38, 1109, 1927, 2274, 2287
ClaI	ATCGAT	1 cut(s)	1152
CseI	GACGC	3 cut(s)	295, 2331, 2497
Csp6I	GTAC	2 cut(s)	1464, 1489
CviQI	GTAC	2 cut(s)	1464, 1489
DraIII	CACNNNGTG	2 cut(s)	1511, 1693
DrdI	GACNNNNNNGTC	1 cut(s)	292
DriI	GACNNNNNGTC	1 cut(s)	1660
DseDI	GACNNNNNNGTC	1 cut(s)	292
Eam1104I	CTCTTC	1 cut(s)	94
Eam1105I	GACNNNNNGTC	1 cut(s)	1660
EarI	CTCTTC	1 cut(s)	94
EciI	GGCGGA	2 cut(s)	205, 288
Ecl136II	GAGCTC	2 cut(s)	1539, 1610
Eco130I	CCWWGG	2 cut(s)	2129, 2166
Eco147I	AGGCCT	1 cut(s)	2165
Eco24I	GRGCYC	2 cut(s)	1541, 1612
Eco31I	GGTCTC	1 cut(s)	270
Eco32I	GATATC	1 cut(s)	730
Eco47I	GGWCC	4 cut(s)	38, 1109, 2274, 2287
Eco53kI	GAGCTC	2 cut(s)	1539, 1610
Eco57I	CTGAAG	3 cut(s)	950, 2444, 2538
Eco81I	CCTNAGG	1 cut(s)	689
Eco88I	CYCGRG	1 cut(s)	2058
Eco91I	GGTNACC	2 cut(s)	1072, 1454
EcoICRI	GAGCTC	2 cut(s)	1539, 1610
EcoNI	CCTNNNNNAGG	1 cut(s)	1581
EcoO109I	RGGNCCY	1 cut(s)	1109
EcoO65I	GGTNACC	2 cut(s)	1072, 1454
EcoRII	CCWGG	3 cut(s)	280, 1054, 1459
EcoRV	GATATC	1 cut(s)	730
EcoT14I	CCWWGG	2 cut(s)	2129, 2166
EcoT38I	GRGCYC	2 cut(s)	1541, 1612
ErhI	CCWWGG	2 cut(s)	2129, 2166
Esp3I	CGTCTC	1 cut(s)	179
FaqI	GGGAC	4 cut(s)	298, 1122, 1459, 2287
FauI	CCCGC	3 cut(s)	103, 2015, 2322
FbaI	TGATCA	1 cut(s)	2148
Fnu4HI	GCNGC	6 cut(s)	99, 219, 1025, 1481, 2043, 2246
FokI	GGATG	5 cut(s)	169, 1223, 1364, 2007, 2188
FriOI	GRGCYC	2 cut(s)	1541, 1612
Fsp4HI	GCNGC	6 cut(s)	99, 219, 1025, 1481, 2043, 2246
FspBI	CTAG	8 cut(s)	441, 920, 1037, 1328, 1782, 1851, 2130, 2492
GlaI	GCGC	2 cut(s)	2180, 2399
GluI	GCNGC	6 cut(s)	99, 219, 1025, 1481, 2043, 2246
GsaI	CCCAGC	1 cut(s)	1480
GsuI	CTGGAG	3 cut(s)	1469, 2322, 2387
HaeIII	GGCC	2 cut(s)	1928, 2165
HapII	CCGG	4 cut(s)	51, 115, 1077, 1804
HgaI	GACGC	3 cut(s)	295, 2331, 2497
HhaI	GCGC	2 cut(s)	2181, 2400
Hin1I	GRCGYC	1 cut(s)	287
Hin6I	GCGC	2 cut(s)	2179, 2398
HinP1I	GCGC	2 cut(s)	2179, 2398
HincII	GTYRAC	2 cut(s)	1216, 2055
HindII	GTYRAC	2 cut(s)	1216, 2055
HindIII	AAGCTT	1 cut(s)	1499
HinfI	GANTC	5 cut(s)	11, 985, 1854, 1873, 2529
HpaII	CCGG	4 cut(s)	51, 115, 1077, 1804
Hpy166II	GTNNAC	6 cut(s)	297, 925, 1216, 2055, 2212, 2318
Hpy8I	GTNNAC	6 cut(s)	297, 925, 1216, 2055, 2212, 2318
Hpy99I	CGWCG	5 cut(s)	38, 114, 350, 2347, 2491
HpyCH4IV	ACGT	4 cut(s)	60, 172, 1792, 2208
HpySE526I	ACGT	4 cut(s)	60, 172, 1792, 2208
Hsp92I	GRCGYC	1 cut(s)	287
HspAI	GCGC	2 cut(s)	2179, 2398
KroI	GCCGGC	1 cut(s)	114
KroNI	GCCGGC	1 cut(s)	116
Ksp22I	TGATCA	1 cut(s)	2148
LmnI	GCTCC	4 cut(s)	1488, 1536, 1607, 1677
Lsp1109I	GCAGC	4 cut(s)	1011, 1467, 2029, 2257
LweI	GCATC	8 cut(s)	188, 523, 800, 923, 1418, 1748, 2166, 2364
MaeI	CTAG	8 cut(s)	441, 920, 1037, 1328, 1782, 1851, 2130, 2492
MaeII	ACGT	4 cut(s)	60, 172, 1792, 2208
MaeIII	GTNAC	6 cut(s)	824, 1072, 1430, 1454, 1622, 2409
MflI	RGATCY	4 cut(s)	125, 224, 1201, 1573
MhlI	GDGCHC	4 cut(s)	927, 1541, 1612, 1957
MmeI	TCCRAC	4 cut(s)	224, 383, 1329, 1862
MreI	CGCCGGCG	1 cut(s)	114
MroNI	GCCGGC	1 cut(s)	114
MroXI	GAANNNNTTC	1 cut(s)	2505
MseI	TTAA	8 cut(s)	399, 423, 645, 684, 1140, 1184, 1359, 1553
MslI	CAYNNNNRTG	4 cut(s)	249, 386, 2282, 2463
MspA1I	CMGCKG	2 cut(s)	1480, 1544
MspI	CCGG	4 cut(s)	51, 115, 1077, 1804
MspR9I	CCNGG	5 cut(s)	51, 282, 1056, 1461, 1804
Mva1269I	GAATGC	1 cut(s)	1399
MvaI	CCWGG	3 cut(s)	282, 1056, 1461
MvnI	CGCG	1 cut(s)	2486
NaeI	GCCGGC	1 cut(s)	116
NciI	CCSGG	2 cut(s)	51, 1804
NgoMIV	GCCGGC	1 cut(s)	114
NheI	GCTAGC	1 cut(s)	2491
NlaIV	GGNNCC	3 cut(s)	1110, 1203, 2275
NmuCI	GTSAC	4 cut(s)	1072, 1430, 1454, 1622
NruI	TCGCGA	1 cut(s)	2486
NspI	RCATGY	2 cut(s)	668, 1684
OliI	CACNNNNGTG	1 cut(s)	249
PaeI	GCATGC	1 cut(s)	1684
PagI	TCATGA	1 cut(s)	2464
PasI	CCCWGGG	1 cut(s)	281
PceI	AGGCCT	1 cut(s)	2165
PciI	ACATGT	1 cut(s)	664
PcsI	WCGNNNNNNNCGW	2 cut(s)	345, 609
PctI	GAATGC	1 cut(s)	1399
PdiI	GCCGGC	1 cut(s)	116
PdmI	GAANNNNTTC	1 cut(s)	2505
PfeI	GAWTC	5 cut(s)	11, 985, 1854, 1873, 2529
PfoI	TCCNGGA	1 cut(s)	49
PkrI	GCNGC	6 cut(s)	100, 220, 1026, 1482, 2044, 2247
PpuMI	RGGWCCY	1 cut(s)	1109
PscI	ACATGT	1 cut(s)	664
PshAI	GACNNNNGTC	1 cut(s)	1221
PshBI	ATTAAT	1 cut(s)	645
PsiI	TTATAA	1 cut(s)	1797
Psp124BI	GAGCTC	2 cut(s)	1541, 1612
Psp1406I	AACGTT	1 cut(s)	60
Psp5II	RGGWCCY	1 cut(s)	1109
Psp6I	CCWGG	3 cut(s)	280, 1054, 1459
PspEI	GGTNACC	2 cut(s)	1072, 1454
PspFI	CCCAGC	1 cut(s)	1476
PspGI	CCWGG	3 cut(s)	280, 1054, 1459
PspN4I	GGNNCC	3 cut(s)	1110, 1203, 2275
PspPI	GGNCC	5 cut(s)	38, 1109, 1927, 2274, 2287
PspPPI	RGGWCCY	1 cut(s)	1109
PstI	CTGCAG	1 cut(s)	2047
PsuI	RGATCY	4 cut(s)	125, 224, 1201, 1573
PvuII	CAGCTG	2 cut(s)	1480, 1544
RruI	TCGCGA	1 cut(s)	2486
RsaI	GTAC	2 cut(s)	1465, 1490
RsaNI	GTAC	2 cut(s)	1464, 1489
RseI	CAYNNNNRTG	4 cut(s)	249, 386, 2282, 2463
SacI	GAGCTC	2 cut(s)	1541, 1612
SaqAI	TTAA	8 cut(s)	399, 423, 645, 684, 1140, 1184, 1359, 1553
SatI	GCNGC	6 cut(s)	99, 219, 1025, 1481, 2043, 2246
Sau96I	GGNCC	5 cut(s)	38, 1109, 1927, 2274, 2287
ScrFI	CCNGG	5 cut(s)	51, 282, 1056, 1461, 1804
SduI	GDGCHC	4 cut(s)	927, 1541, 1612, 1957
SfaNI	GCATC	8 cut(s)	188, 523, 800, 923, 1418, 1748, 2166, 2364
SfcI	CTRYAG	2 cut(s)	1968, 2043
SgrAI	CRCCGGYG	1 cut(s)	114
SinI	GGWCC	4 cut(s)	38, 1109, 2274, 2287
SmiMI	CAYNNNNRTG	4 cut(s)	249, 386, 2282, 2463
SpeI	ACTAGT	1 cut(s)	919
SphI	GCATGC	1 cut(s)	1684
SseBI	AGGCCT	1 cut(s)	2165
SspI	AATATT	1 cut(s)	1825
SspMI	CTAG	8 cut(s)	441, 920, 1037, 1328, 1782, 1851, 2130, 2492
SstI	GAGCTC	2 cut(s)	1541, 1612
StuI	AGGCCT	1 cut(s)	2165
StyD4I	CCNGG	5 cut(s)	49, 280, 1054, 1459, 1802
StyI	CCWWGG	2 cut(s)	2129, 2166
TaiI	ACGT	4 cut(s)	63, 175, 1795, 2211
TaqI	TCGA	9 cut(s)	75, 488, 612, 657, 732, 1152, 1248, 1857, 2224
TaqII	GACCGA	2 cut(s)	1641, 1917
TatI	WGTACW	1 cut(s)	1488
TauI	GCSGC	2 cut(s)	101, 221
TfiI	GAWTC	5 cut(s)	11, 985, 1854, 1873, 2529
Tru1I	TTAA	8 cut(s)	399, 423, 645, 684, 1140, 1184, 1359, 1553
Tru9I	TTAA	8 cut(s)	399, 423, 645, 684, 1140, 1184, 1359, 1553
TscAI	CASTG	5 cut(s)	304, 315, 391, 1675, 1989
TseFI	GTSAC	4 cut(s)	1072, 1430, 1454, 1622
TseI	GCWGC	4 cut(s)	1024, 1480, 2042, 2245
Tsp45I	GTSAC	4 cut(s)	1072, 1430, 1454, 1622
TspDTI	ATGAA	7 cut(s)	1254, 1484, 1662, 2009, 2052, 2447, 2453
TspGWI	ACGGA	3 cut(s)	261, 354, 2273
TspRI	CASTG	5 cut(s)	304, 315, 391, 1675, 1989
VneI	GTGCAC	1 cut(s)	923
VpaK11BI	GGWCC	4 cut(s)	38, 1109, 2274, 2287
VspI	ATTAAT	1 cut(s)	645
XagI	CCTNNNNNAGG	1 cut(s)	1581
XapI	RAATTY	3 cut(s)	1080, 1649, 2454
XbaI	TCTAGA	1 cut(s)	1850
XceI	RCATGY	2 cut(s)	668, 1684
XmaJI	CCTAGG	1 cut(s)	2129
XmnI	GAANNNNTTC	1 cut(s)	2505
XspI	CTAG	8 cut(s)	441, 920, 1037, 1328, 1782, 1851, 2130, 2492

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.