Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0008419

Physical Location & Seq

Genomic Coordinates Forward (+)

23431770 .. 23437792

Gene Structure 6023 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG149000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2511 bp

ATGGCTTCCGACTCTACTGCACCCAATTTCAACAACGGGCCTTCCTCTCCCGATGACTCCTTCTCCAGCCCAATCGGCAACACCTTCTCTTCTCCAGCCGACGCCGCCGCCGCCGCCTCTCGCCAGAGAAGCCGCCGCCGATCCACCACACCCACCGCATTCGCAACCCCTCCCGCCTCCCGCTCCCGCTTCGCCGCCTCCGAAACAACCCCAACCCCATCATCTCGCACGCGCCGCCCCAACGGCACCGCACGCACCCCCTCCTCTACCGATGATGGCCCCCCCTCTTCGGAAGGCGGCGACAGCTACGGCATGGACGAGGACCGCCCCACCTTCGTCTGGGGCACCAATATCAGCGTCAATGATGTTAAGCTCGCCATTATTAGGTTTTTGAAGAATTTTCGGGACCAATCGCAGAACTCCGAGAGCGATTATCTCACCGAAGGGAAATACATTGAGGCCATCAAGAGAATTCTGGAAATGGAAGGCGATTCACTGGATGTGGATGCCCGCAATGTGTTTGATTATGATTCCGATTTGTATGCTAAGATGGTCAGGTACCCACTTGAGGTTCTTGCTATTTTCGACATTGTTTTGATGGAATTGTTGCCTGTCATAAACCCCTTGTTCGAGAAGCACATTCAGACTCGCATTTTCAATCTCAAAACATCAACATCCATGAGAAATCTGAACCCGTCTGATATTGAGAGGATGGTTTCAATAAAGGGGATGATTATTCGGTCCAGTTCAATAATACCTGAGATAAGGGAAGCAATATTTAGATGCCTTGTGTGTGGTTACTACTCTGACCCTCTTCCTGTAGAAAAAGGGAAAATAAATGAACCTACAAGATGCATGAAGGAAGAGTGTCAAGCCAAGAATTCTATGACACTTGTTCACAACCGTTGCAGGTTTACTGATAAACAGATTGTGAGGCTCCAGGAGACGCCTGATGAGATCCCTGAAGGAGGGACACCGCATACTGTGAGTTTGTTGATGCATGACAAACTGGTTGATGCGGGAAAACCAGGTGACAGAGTTGAGGTTACTGGGATATATCGGGCTATGAGCGTAAGAGTTGGACCTACACAGAGAACTGTAAAATCATTATTCAAGACTTATATTGATTGTCTGCATATAAAAAAGTCTGACAAGTCAAGAATGCTGGCGGAGGAGCAGACGGAAGTTGAGAATTCCTTGGGTGGAAATTCAGATGAGATTCTTTTTGATGAAAAGAAGGTGGAACAATTGAAGGAGCTGGCAAAACAGCCTGATATATATGATCGCCTAACCAGATCTTTGGCACCAAACATCTGGGAGTTAGATGATGTCAAGAAAGGCCTTCTTTGCCAGCTTTTTGGTGGGAATGCTTTGAAGTTGCCATCGGGTGCAAGGTTCCGTGGGGATATTAATATTCTTCTTGTTGGTGATCCTGGAACCAGCAAGTCCCAGCTGCTCCAGTACATACACAAGCTTGCTCCCCGTGGCATATACACTAGTGGAAGAGGGAGTTCTGCTGTTGGTTTGACTGCTTATGTTGCAAAAGATCCTGAAACAGGGGAAACTGTTTTAGAGAGTGGAGCCTTAGTTTTGAGTGATCGAGGCATTTGTTGCATTGATGAATTTGACAAAATGTCTGACAGTGCAAGGAGCATGTTACACGAGGTGATGGAGCAGCAAACTGTGTCAATAGCAAAGGCAGGAATCATTGCTTCCCTTAATGCTAGGACATCAGTCCTGGCTTGTGCAAATCCAAGTGGATCTCGCTATAATCCTCGCTTGTCAGTAATTGACAACATACACCTTCCTCCTACCTTATTATCCAGGTTTGATCTGATCTACTTAATCCTTGACAAGGCTGATGAGCAGACAGACAGGCGCCTTGCAAAGCATATTGTTGCCTTGCACTTTGAGAATCCTGAGACTGTACAGCAGGATGTTTTAGACCTTGCCACGTTGACTGCTTATGTGGGATATGCCCGAAAGCATATTCACCCACAATTATCTGATGAAGCTGCAGAGGAGTTGACTCGTGGCTATGTAGAGTTGAGGAGGAGAGGAAATTTTCCGGGCAGCAGCAAAAAGGTGATAACAGCAACTCCTAGGCAAATCGAGAGTTTGATACGTCTTAGTGAAGCTCTAGCTCGGATTCGTTTTTCAGAATCGGTTGAAAAGCATGATGTAATGGAGGCATTTCGGCTTCTTGAAGTTGCAATGCAACAATCAGCAACAGATCACTCCACTGGAACAATCGACATGGATCTTATTAATACTGGAATTTCGGCAAGTGAAAGAATCAGACGGGAGAATCTAGTGTCATCAGCTCGCAACTTAATTATGGAGAAGATGCAACTTGGGGGACCCTCAATGCGTTTGTTAGAGCTGCTGGAGGAGCTGAAGAAGCAGAGTTCTGGCAGTGAAATTCACCTCAACGATCTTAGGAATGCAATTGCTACGCTTGCAAGTGAGGGATTTGTCGCTGTTCATGGAGACAGTGTCAAACGAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0000785 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

836

Amino Acids

92.7

Weight (kDa)

6.14

Isoelectric Point (pI)

51.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	127 - 219	1.2e-11	MCM N-terminal domain
MCM_OB	PF17207	227 - 356	2.4e-40	MCM OB domain
MCM	PF00493	414 - 636	3.9e-101	MCM P-loop domain
AAA_5	PF07728	471 - 587	7.9e-07	AAA domain (dynein-related subfamily)
MCM_lid	PF17855	652 - 738	1.1e-26	MCM AAA-lid domain
WHD_MCM4	PF21128	762 - 836	1.4e-06	MCM4, winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008419)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16440
fragaria_vesca	FvH4_2g09990
malus_domestica	MD00G1085600.v1.1 MD10G1066500.v1.1
prunus_persica	Prupe.8G088400_v2.0.a1
pyrus_communis	pycom05g04970 pycom05g04980 pycom10g05200
rosa_chinensis	RchiOBHm_Chr6g0266881 RchiOBHm_Chr6g0266891
rosa_laevigata	RLG00000014063 RLG00000014064 RLG00000014065
rosa_multiflora	Rmu_co8507775.1_g000001 Rmu_sc0021142.1_g000001 Rmu_sc0036177.1_g000001
rosa_roxburghii	Rroxscaffold_7G00201330
rosa_rugosa	Rorug06G0027900
rosa_samantha	Rh6AG149000 Rh6BG150000 Rh6CG144900 Rh6DG134200
rosa_wichuraiana	Rw6G012890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	900
Acc65I	GGTACC	1 cut(s)	558
AccB1I	GGYRCC	5 cut(s)	245, 344, 558, 1303, 1878
AccBSI	CCGCTC	1 cut(s)	183
AccII	CGCG	1 cut(s)	232
AclWI	GGATC	6 cut(s)	135, 952, 1424, 1541, 1768, 2268
AcsI	RAATTY	9 cut(s)	397, 471, 880, 1192, 1207, 1622, 2062, 2277, 2421
AcuI	CTGAAG	2 cut(s)	984, 2417
AcyI	GRCGYC	3 cut(s)	102, 947, 1879
AdeI	CACNNNGTG	1 cut(s)	1666
AfaI	GTAC	3 cut(s)	560, 1463, 1929
AfiI	CCNNNNNNNGG	7 cut(s)	289, 339, 384, 568, 968, 1556, 1855
AhdI	GACNNNNNGTC	1 cut(s)	1633
AhlI	ACTAGT	1 cut(s)	1496
AjnI	CCWGG	5 cut(s)	939, 1027, 1432, 1737, 1823
Alw26I	GTCTC	3 cut(s)	938, 1916, 2484
AlwI	GGATC	6 cut(s)	135, 952, 1424, 1541, 1768, 2268
AoxI	GGCC	4 cut(s)	38, 277, 459, 1339
ApeKI	GCWGC	6 cut(s)	1453, 1675, 2015, 2073, 2076, 2383
ApoI	RAATTY	9 cut(s)	397, 471, 880, 1192, 1207, 1622, 2062, 2277, 2421
AseI	ATTAAT	2 cut(s)	1410, 2268
Asp718I	GGTACC	1 cut(s)	558
AspA2I	CCTAGG	1 cut(s)	2102
AspLEI	GCGC	2 cut(s)	234, 1881
AspS9I	GGNCC	7 cut(s)	38, 278, 322, 406, 741, 1082, 2360
AsuC2I	CCSGG	1 cut(s)	2070
AsuHPI	GGTGA	7 cut(s)	430, 1043, 1439, 1678, 1985, 2098, 2417
AvaII	GGWCC	5 cut(s)	322, 406, 741, 1082, 2360
AvrII	CCTAGG	1 cut(s)	2102
BaeGI	GKGCMC	1 cut(s)	347
BanI	GGYRCC	5 cut(s)	245, 344, 558, 1303, 1878
BauI	CACGAG	2 cut(s)	1661, 2031
BbvI	GCAGC	6 cut(s)	1440, 1687, 2002, 2085, 2088, 2370
BccI	CCATC	8 cut(s)	226, 269, 470, 544, 592, 706, 1390, 1663
BceAI	ACGGC	2 cut(s)	259, 325
BcgI	CGANNNNNNTGC	3 cut(s)	33, 524, 558
BciT130I	CCWGG	5 cut(s)	941, 1029, 1434, 1739, 1825
BcnI	CCSGG	1 cut(s)	2070
BcoDI	GTCTC	3 cut(s)	938, 1916, 2484
BcuI	ACTAGT	1 cut(s)	1496
BfaI	CTAG	5 cut(s)	1497, 1725, 2103, 2141, 2312
BfmI	CTRYAG	2 cut(s)	819, 2016
BfoI	RGCGCY	1 cut(s)	1882
BfuAI	ACCTGC	1 cut(s)	900
BglI	GCCNNNNNGGC	2 cut(s)	75, 243
BglII	AGATCT	1 cut(s)	1295
BlnI	CCTAGG	1 cut(s)	2102
Bme1390I	CCNGG	6 cut(s)	941, 1029, 1434, 1739, 1825, 2070
Bme18I	GGWCC	5 cut(s)	322, 406, 741, 1082, 2360
BmeRI	GACNNNNNGTC	1 cut(s)	1633
BmgT120I	GGNCC	7 cut(s)	38, 278, 322, 406, 741, 1082, 2360
BmrFI	CCNGG	6 cut(s)	941, 1029, 1434, 1739, 1825, 2070
BmrI	ACTGGG	1 cut(s)	1059
BmsI	GCATC	6 cut(s)	496, 773, 842, 987, 1006, 2337
BmuI	ACTGGG	1 cut(s)	1059
BoxI	GACNNNNGTC	1 cut(s)	1733
BpmI	CTGGAG	5 cut(s)	49, 78, 923, 1442, 2408
BpuEI	CTTGAG	1 cut(s)	587
BpuMI	CCSGG	1 cut(s)	2070
BsaHI	GRCGYC	3 cut(s)	102, 947, 1879
BsaJI	CCNNGG	4 cut(s)	1197, 1399, 1483, 2102
BsaXI	ACNNNNNCTCC	8 cut(s)	47, 77, 1572, 1602, 2083, 2113, 2481, 2511
Bsc4I	CCNNNNNNNGG	7 cut(s)	289, 339, 384, 568, 968, 1556, 1855
Bse1I	ACTGG	7 cut(s)	501, 744, 1014, 1054, 1459, 2248, 2278
Bse3DI	GCAATG	3 cut(s)	520, 1707, 2220
BseBI	CCWGG	5 cut(s)	941, 1029, 1434, 1739, 1825
BseDI	CCNNGG	4 cut(s)	1197, 1399, 1483, 2102
BseGI	GGATG	6 cut(s)	505, 511, 674, 717, 735, 1942
BseLI	CCNNNNNNNGG	7 cut(s)	289, 339, 384, 568, 968, 1556, 1855
BseMI	GCAATG	3 cut(s)	520, 1707, 2220
BseMII	CTCAG	2 cut(s)	750, 1911
BseNI	ACTGG	7 cut(s)	501, 744, 1014, 1054, 1459, 2248, 2278
BseRI	GAGGAG	6 cut(s)	253, 1187, 2036, 2065, 2068, 2405
BseSI	GKGCMC	1 cut(s)	347
BseXI	GCAGC	6 cut(s)	1440, 1687, 2002, 2085, 2088, 2370
BseYI	CCCAGC	1 cut(s)	1449
Bsh1236I	CGCG	1 cut(s)	232
BshFI	GGCC	4 cut(s)	40, 279, 461, 1341
BshNI	GGYRCC	5 cut(s)	245, 344, 558, 1303, 1878
BsiSI	CCGG	1 cut(s)	2069
BslFI	GGGAC	4 cut(s)	419, 985, 1432, 2373
BslI	CCNNNNNNNGG	7 cut(s)	289, 339, 384, 568, 968, 1556, 1855
BsmAI	GTCTC	3 cut(s)	938, 1916, 2484
BsmBI	CGTCTC	1 cut(s)	938
BsmFI	GGGAC	4 cut(s)	419, 985, 1432, 2373
BsmI	GAATGC	4 cut(s)	158, 1167, 1372, 2449
BsnI	GGCC	4 cut(s)	40, 279, 461, 1341
Bsp1286I	GDGCHC	1 cut(s)	347
Bsp1407I	TGTACA	1 cut(s)	1927
BspANI	GGCC	4 cut(s)	40, 279, 461, 1341
BspCNI	CTCAG	2 cut(s)	751, 1912
BspFNI	CGCG	1 cut(s)	232
BspMAI	CTGCAG	1 cut(s)	2020
BspMI	ACCTGC	1 cut(s)	900
BspPI	GGATC	6 cut(s)	135, 952, 1424, 1541, 1768, 2268
BspT107I	GGYRCC	5 cut(s)	245, 344, 558, 1303, 1878
BsrBI	CCGCTC	1 cut(s)	183
BsrDI	GCAATG	3 cut(s)	520, 1707, 2220
BsrGI	TGTACA	1 cut(s)	1927
BsrI	ACTGG	7 cut(s)	501, 744, 1014, 1054, 1459, 2248, 2278
BssECI	CCNNGG	4 cut(s)	1197, 1399, 1483, 2102
BssNI	GRCGYC	3 cut(s)	102, 947, 1879
BssSI	CACGAG	2 cut(s)	1661, 2031
BssT1I	CCWWGG	2 cut(s)	1197, 2102
Bst2BI	CACGAG	2 cut(s)	1661, 2031
Bst2UI	CCWGG	5 cut(s)	941, 1029, 1434, 1739, 1825
Bst4CI	ACNGT	8 cut(s)	905, 985, 1099, 1567, 1643, 1684, 1927, 2495
Bst6I	CTCTTC	5 cut(s)	94, 292, 819, 858, 1498
BstACI	GRCGYC	3 cut(s)	102, 947, 1879
BstAPI	GCANNNNNTGC	1 cut(s)	1611
BstAUI	TGTACA	1 cut(s)	1927
BstDEI	CTNAG	6 cut(s)	546, 759, 1585, 1920, 2129, 2438
BstDSI	CCRYGG	2 cut(s)	1399, 1483
BstENI	CCTNNNNNAGG	3 cut(s)	966, 1554, 1853
BstF5I	GGATG	6 cut(s)	505, 511, 674, 717, 735, 1942
BstFNI	CGCG	1 cut(s)	232
BstH2I	RGCGCY	1 cut(s)	1882
BstHHI	GCGC	2 cut(s)	234, 1881
BstMAI	GTCTC	3 cut(s)	938, 1916, 2484
BstNI	CCWGG	5 cut(s)	941, 1029, 1434, 1739, 1825
BstNSI	RCATGY	1 cut(s)	1657
BstPAI	GACNNNNGTC	1 cut(s)	1733
BstSCI	CCNGG	6 cut(s)	939, 1027, 1432, 1737, 1823, 2068
BstSFI	CTRYAG	2 cut(s)	819, 2016
BstSLI	GKGCMC	1 cut(s)	347
BstUI	CGCG	1 cut(s)	232
BstV1I	GCAGC	6 cut(s)	1440, 1687, 2002, 2085, 2088, 2370
BstX2I	RGATCY	5 cut(s)	957, 1295, 1546, 1760, 2260
BstXI	CCANNNNNNTGG	3 cut(s)	1300, 1314, 1358
BstYI	RGATCY	5 cut(s)	957, 1295, 1546, 1760, 2260
BsuRI	GGCC	4 cut(s)	40, 279, 461, 1341
BtgI	CCRYGG	2 cut(s)	1399, 1483
BtsCI	GGATG	6 cut(s)	505, 511, 674, 717, 735, 1942
BtsI	GCAGTG	1 cut(s)	2422
BtsIMutI	CAGTG	5 cut(s)	494, 1648, 2241, 2422, 2500
BveI	ACCTGC	1 cut(s)	900
CfoI	GCGC	2 cut(s)	234, 1881
Cfr13I	GGNCC	7 cut(s)	38, 278, 322, 406, 741, 1082, 2360
CseI	GACGC	3 cut(s)	110, 346, 955
CsiI	ACCWGGT	1 cut(s)	1027
Csp6I	GTAC	3 cut(s)	559, 1462, 1928
CviAII	CATG	8 cut(s)	313, 679, 856, 1001, 1654, 2177, 2257, 2486
CviQI	GTAC	3 cut(s)	559, 1462, 1928
DdeI	CTNAG	6 cut(s)	546, 759, 1585, 1920, 2129, 2438
DinI	GGCGCC	1 cut(s)	1880
DraIII	CACNNNGTG	1 cut(s)	1666
DriI	GACNNNNNGTC	1 cut(s)	1633
Eam1104I	CTCTTC	5 cut(s)	94, 292, 819, 858, 1498
Eam1105I	GACNNNNNGTC	1 cut(s)	1633
EarI	CTCTTC	5 cut(s)	94, 292, 819, 858, 1498
EciI	GGCGGA	1 cut(s)	1184
Eco130I	CCWWGG	2 cut(s)	1197, 2102
Eco147I	AGGCCT	1 cut(s)	1341
Eco47I	GGWCC	5 cut(s)	322, 406, 741, 1082, 2360
Eco57I	CTGAAG	2 cut(s)	984, 2417
EcoNI	CCTNNNNNAGG	3 cut(s)	966, 1554, 1853
EcoO109I	RGGNCCY	1 cut(s)	2360
EcoRI	GAATTC	3 cut(s)	471, 880, 1192
EcoRII	CCWGG	5 cut(s)	939, 1027, 1432, 1737, 1823
EcoT14I	CCWWGG	2 cut(s)	1197, 2102
EcoT22I	ATGCAT	2 cut(s)	857, 1002
EgeI	GGCGCC	1 cut(s)	1880
EheI	GGCGCC	1 cut(s)	1880
ErhI	CCWWGG	2 cut(s)	1197, 2102
Esp3I	CGTCTC	1 cut(s)	938
FaeI	CATG	8 cut(s)	316, 682, 859, 1004, 1657, 2180, 2260, 2489
FalI	AAGNNNNNCTT	2 cut(s)	1329, 1361
FaqI	GGGAC	4 cut(s)	419, 985, 1432, 2373
FatI	CATG	8 cut(s)	312, 678, 855, 1000, 1653, 2176, 2256, 2485
FauI	CCCGC	5 cut(s)	181, 188, 194, 518, 1012
FokI	GGATG	6 cut(s)	512, 518, 661, 724, 742, 1949
FspBI	CTAG	5 cut(s)	1497, 1725, 2103, 2141, 2312
GlaI	GCGC	2 cut(s)	233, 1880
GsaI	CCCAGC	1 cut(s)	1453
GsuI	CTGGAG	5 cut(s)	49, 78, 923, 1442, 2408
HaeII	RGCGCY	1 cut(s)	1882
HaeIII	GGCC	4 cut(s)	40, 279, 461, 1341
HapII	CCGG	1 cut(s)	2069
HgaI	GACGC	3 cut(s)	110, 346, 955
HhaI	GCGC	2 cut(s)	234, 1881
Hin1I	GRCGYC	3 cut(s)	102, 947, 1879
Hin1II	CATG	8 cut(s)	316, 682, 859, 1004, 1657, 2180, 2260, 2489
Hin6I	GCGC	2 cut(s)	232, 1879
HinP1I	GCGC	2 cut(s)	232, 1879
HincII	GTYRAC	2 cut(s)	1959, 2028
HindII	GTYRAC	2 cut(s)	1959, 2028
HindIII	AAGCTT	1 cut(s)	1472
HpaII	CCGG	1 cut(s)	2069
HphI	GGTGA	7 cut(s)	430, 1043, 1439, 1678, 1985, 2098, 2417
Hpy166II	GTNNAC	4 cut(s)	898, 915, 1959, 2028
Hpy8I	GTNNAC	4 cut(s)	898, 915, 1959, 2028
Hpy99I	CGWCG	1 cut(s)	104
HpyCH4III	ACNGT	8 cut(s)	905, 985, 1099, 1567, 1643, 1684, 1927, 2495
HpyCH4IV	ACGT	2 cut(s)	1955, 2125
HpyF3I	CTNAG	6 cut(s)	546, 759, 1585, 1920, 2129, 2438
HpySE526I	ACGT	2 cut(s)	1955, 2125
Hsp92I	GRCGYC	3 cut(s)	102, 947, 1879
Hsp92II	CATG	8 cut(s)	316, 682, 859, 1004, 1657, 2180, 2260, 2489
HspAI	GCGC	2 cut(s)	232, 1879
KasI	GGCGCC	1 cut(s)	1878
KflI	GGGWCCC	1 cut(s)	2360
KpnI	GGTACC	1 cut(s)	562
Lsp1109I	GCAGC	6 cut(s)	1440, 1687, 2002, 2085, 2088, 2370
LweI	GCATC	6 cut(s)	496, 773, 842, 987, 1006, 2337
MabI	ACCWGGT	1 cut(s)	1027
MaeI	CTAG	5 cut(s)	1497, 1725, 2103, 2141, 2312
MaeII	ACGT	2 cut(s)	1955, 2125
MaeIII	GTNAC	4 cut(s)	797, 1031, 1045, 1656
MbiI	CCGCTC	1 cut(s)	183
MboII	GAAGA	9 cut(s)	81, 279, 406, 806, 875, 1409, 1515, 2356, 2410
MfeI	CAATTG	2 cut(s)	1247, 2448
MflI	RGATCY	5 cut(s)	957, 1295, 1546, 1760, 2260
MhlI	GDGCHC	1 cut(s)	347
Mly113I	GGCGCC	1 cut(s)	1879
MlyI	GAGTC	4 cut(s)	5, 50, 640, 2023
MmeI	TCCRAC	2 cut(s)	33, 1060
Mph1103I	ATGCAT	2 cut(s)	857, 1002
MseI	TTAA	6 cut(s)	369, 1410, 1719, 1844, 2268, 2333
MspA1I	CMGCKG	1 cut(s)	1453
MspI	CCGG	1 cut(s)	2069
MspR9I	CCNGG	6 cut(s)	941, 1029, 1434, 1739, 1825, 2070
MunI	CAATTG	2 cut(s)	1247, 2448
Mva1269I	GAATGC	4 cut(s)	158, 1167, 1372, 2449
MvaI	CCWGG	5 cut(s)	941, 1029, 1434, 1739, 1825
MvnI	CGCG	1 cut(s)	232
NarI	GGCGCC	1 cut(s)	1879
NciI	CCSGG	1 cut(s)	2070
NlaIII	CATG	8 cut(s)	316, 682, 859, 1004, 1657, 2180, 2260, 2489
NmuCI	GTSAC	1 cut(s)	1031
NsiI	ATGCAT	2 cut(s)	857, 1002
NspI	RCATGY	1 cut(s)	1657
PceI	AGGCCT	1 cut(s)	1341
PcsI	WCGNNNNNNNCGW	2 cut(s)	198, 315
PctI	GAATGC	4 cut(s)	158, 1167, 1372, 2449
PfeI	GAWTC	9 cut(s)	491, 530, 1219, 1704, 1915, 2149, 2162, 2295, 2308
PflFI	GACNNNGTC	1 cut(s)	2495
PfoI	TCCNGGA	2 cut(s)	939, 1432
PleI	GAGTC	4 cut(s)	5, 50, 640, 2023
PluTI	GGCGCC	1 cut(s)	1882
PpsI	GAGTC	4 cut(s)	5, 50, 640, 2023
PpuMI	RGGWCCY	1 cut(s)	2360
PshAI	GACNNNNGTC	1 cut(s)	1733
PshBI	ATTAAT	2 cut(s)	1410, 2268
Psp5II	RGGWCCY	1 cut(s)	2360
Psp6I	CCWGG	5 cut(s)	939, 1027, 1432, 1737, 1823
PspFI	CCCAGC	1 cut(s)	1449
PspGI	CCWGG	5 cut(s)	939, 1027, 1432, 1737, 1823
PspPI	GGNCC	7 cut(s)	38, 278, 322, 406, 741, 1082, 2360
PspPPI	RGGWCCY	1 cut(s)	2360
PstI	CTGCAG	1 cut(s)	2020
PsuI	RGATCY	5 cut(s)	957, 1295, 1546, 1760, 2260
PsyI	GACNNNGTC	1 cut(s)	2495
PvuII	CAGCTG	1 cut(s)	1453
RsaI	GTAC	3 cut(s)	560, 1463, 1929
RsaNI	GTAC	3 cut(s)	559, 1462, 1928
SaqAI	TTAA	6 cut(s)	369, 1410, 1719, 1844, 2268, 2333
Sau96I	GGNCC	7 cut(s)	38, 278, 322, 406, 741, 1082, 2360
SchI	GAGTC	4 cut(s)	5, 50, 640, 2023
ScrFI	CCNGG	6 cut(s)	941, 1029, 1434, 1739, 1825, 2070
SduI	GDGCHC	1 cut(s)	347
SexAI	ACCWGGT	1 cut(s)	1027
SfaNI	GCATC	6 cut(s)	496, 773, 842, 987, 1006, 2337
SfcI	CTRYAG	2 cut(s)	819, 2016
SfoI	GGCGCC	1 cut(s)	1880
SinI	GGWCC	5 cut(s)	322, 406, 741, 1082, 2360
SmlI	CTYRAG	1 cut(s)	566
SmoI	CTYRAG	1 cut(s)	566
SpeI	ACTAGT	1 cut(s)	1496
SseBI	AGGCCT	1 cut(s)	1341
SspDI	GGCGCC	1 cut(s)	1878
SspI	AATATT	2 cut(s)	777, 1414
SspMI	CTAG	5 cut(s)	1497, 1725, 2103, 2141, 2312
StuI	AGGCCT	1 cut(s)	1341
StyD4I	CCNGG	6 cut(s)	939, 1027, 1432, 1737, 1823, 2068
StyI	CCWWGG	2 cut(s)	1197, 2102
TaaI	ACNGT	8 cut(s)	905, 985, 1099, 1567, 1643, 1684, 1927, 2495
TaiI	ACGT	2 cut(s)	1958, 2128
TaqI	TCGA	5 cut(s)	585, 630, 1600, 2112, 2253
TaqII	GACCGA	1 cut(s)	729
TatI	WGTACW	2 cut(s)	1461, 1927
TauI	GCSGC	9 cut(s)	107, 110, 113, 116, 135, 138, 197, 237, 300
TfiI	GAWTC	9 cut(s)	491, 530, 1219, 1704, 1915, 2149, 2162, 2295, 2308
Tru1I	TTAA	6 cut(s)	369, 1410, 1719, 1844, 2268, 2333
Tru9I	TTAA	6 cut(s)	369, 1410, 1719, 1844, 2268, 2333
TscAI	CASTG	5 cut(s)	501, 1648, 2248, 2422, 2500
TseFI	GTSAC	1 cut(s)	1031
TseI	GCWGC	6 cut(s)	1453, 1675, 2015, 2073, 2076, 2383
Tsp45I	GTSAC	1 cut(s)	1031
TspDTI	ATGAA	6 cut(s)	855, 872, 1245, 1635, 2025, 2474
TspGWI	ACGGA	2 cut(s)	1196, 1388
TspRI	CASTG	5 cut(s)	501, 1648, 2248, 2422, 2500
Tth111I	GACNNNGTC	1 cut(s)	2495
VpaK11BI	GGWCC	5 cut(s)	322, 406, 741, 1082, 2360
VspI	ATTAAT	2 cut(s)	1410, 2268
XagI	CCTNNNNNAGG	3 cut(s)	966, 1554, 1853
XapI	RAATTY	9 cut(s)	397, 471, 880, 1192, 1207, 1622, 2062, 2277, 2421
XceI	RCATGY	1 cut(s)	1657
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1358
XmaJI	CCTAGG	1 cut(s)	2102
XspI	CTAG	5 cut(s)	1497, 1725, 2103, 2141, 2312
Zsp2I	ATGCAT	2 cut(s)	857, 1002

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.