Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008419

Physical Location & Seq

Genomic Coordinates Forward (+)

9104269 .. 9110096

Gene Structure 5828 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1066500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2508 bp

ATGGCTTCCGATTCTTCTCCGGCGGACTTCAACAACGGGCCTTCTTCTCCTGATGACTCCTTATCGAGCCCAATCGGCAACACCTTCTCCTCTCCGGCGGACGCCACCCACCGCAGGAGGCGTCGATCCATTACCCCCTCGGAATTTGACACCCCGCGGGCCCAGCGGTCCCGGTTCGCCGCATCCGAAACAACCCCAACGGGCACGCGCCGCGCCAACGGCCATAGTGCTGGCCCTGCCCCGACCCCTTCCTCCACCGACGGTGATGTCCCGCCATCTTCTGAAGGCGGCGACGGGTATGGCATGGATGAGGACCGACCCACATTTGTCTGGGGCACGAATATCAGCGTGAACGACGTTAAGGTGGCGATCGTTAGGTTTCTGAAGAATTTTCGGGATGAGTCGCAGTGGAATACGGAGAGTGAGTACCTTACAGAAGGGAAGTACATTGAGGCCATCAAGAGAGTTATTGAGATGGAAGATGATTCGCTTGATGTTGATGCACGCAATGTGTTTGATTATGATTCCGACTTGTATGCGAAGATGGTCAGGTACCCACTCGAGGTTCTTGCGATTTTTGATATTGTTTTGATGGAAATGCTGCCCATCATAAACCCGTTGTTTGAAAAGCACATCCAAACTCGGATTTATAATCTCAAAACATCCACATCGATGAGAAATCTCAACCCATCTGACATTGAGAGGATGGTTTCGTTGAAGGGAATGATTATTCGGTCTAGTTCAATAATACCAGAGATAAGGGAAGCAATATTTCGATGCCTCGTGTGTGGCTACTACTCCGACCCTCTTCCCGTAGAGAAAGGGAGAATATCTGAGCCTACAATATGCTTGAAGGAAGAGTGTCAAGCCAGGAATTCCATGACACTTGTTCATAATCGATGCAGGTTTACGGATAAACAGATTGTGAGGCTCCAGGAGACTCCGGATGAGATCCCTGAAGGAGGGACACCACATACGGTGAGCTTGTTGATGCATGACAAACTGGTTGATGCTGGAAAACCAGGAGACAGAATTGAGGTTACTGGGATATACAGGGCTATGACTGTTAGAGTTGGACCAACACAGAGAACTGTAAAATCACTGTTCAAGACTTACATTGATTGTCTGCATATAAAAAAATCTGATAAGTCAAGAATGCTGGCGGAGGATCAAGTGGAAGTTGACAATACCTTGGTTGGAAACTCAGAAGAGATTCTTTTTGATGAAAAAAAGGTGGAACAATTGAAAGAGCTGGCAAAACAGCCAGATATATATGATCGATTAACAAGGTCTTTAGCACCAAACATCTGGGAGTTAGATGATGTAAAAAAAGGCCTTCTTTGCCAGCTTTTTGGTGGGAATGCTTTGAAGCTTCCATCTGGTGCTCGCTTTCGTGGTGATATTAATATTCTTCTTGTTGGTGATCCTGGAACCAGCAAGTCCCAGCTGCTCCAGTACATACACAAGTTAGCGCCTCGCGGAATATACACTAGTGGAAGAGGGAGTTCTGCCGTTGGATTGACTGCTTATGTTGCGAAAGATCCCGAAACAGGGGAAACAGTTTTAGAGAGTGGAGCCTTAGTTTTGAGTGATAGAGGCATTTGCTGCATTGACGAATTTGACAAAATGTCTGATAGTGCAAGGAGCATGCTACATGAGGTGATGGAACAGCAAACTGTTTCAATAGCTAAGGCAGGAATTATTGCTTCCCTTAATGCTAGGACTTCGGTCTTGGCTTGTGCAAATCCAAGTGGTTCACGCTATAACCCTCGCTTATCAGTAATTGACAACATACACCTTCCTCCTACCTTATTATCCAGGTTTGATCTGATCTACTTACTCCTTGACAAGGCTGATGAGCATACAGACAGACGCCTCGCAAAGCATATTGTTGCATTACACTTTGAGAATCCTGAGAGTGTACAGCAGAATGTCTTAGACATTGCTACGTTGACTGCATATGTGGGCTATGCCCGAAAGCATATTCACCCACAATTATCTGATGAGGCTGCTGAAGAGTTGACTAGAGGTTATGTTGAGTTGAGGAGGAGAGGAAATTTTCCAGGCAGTAGCAAAAAGGTGATAACAGCAACACCCAGGCAGATTGAGAGTTTGATACGACTTAGTGAAGCTCTAGCTCGCATCCGTTTCTCAGAATCGGTTGAGAAGCATGATGTTATTGAGGCATTTCGGCTTCTGGAAGTTGCAATGCAGCAATCGGCAACTGATCACTCCACCGGAACGATCGACATGGATCTTATCACCACTGGAATTTCGGCAAGTGAAAGAATGAGACGAGAAAGTCTCGTATCATCAGCTCGCAACATAATTATGGAGAAGATGCAACTTGGGGGACCCTCAATGCGCTTGTTAGAGCTGCTGGAGGAGCTGAAGCAGCAGAGCTCTGGCGGCGAACTCCATCTCAATGATCTGAGGACTGCAATTACGACACTAGCAACTGAGGGATTTGTGGCTTTTGTTCACGGCGACAGTGTCAAAAGAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0000785 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

836

Amino Acids

92.9

Weight (kDa)

5.8

Isoelectric Point (pI)

51.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	124 - 218	4.3e-12	MCM N-terminal domain
MCM_OB	PF17207	225 - 354	6.2e-39	MCM OB domain
MCM	PF00493	412 - 634	8.9e-101	MCM P-loop domain
AAA_5	PF07728	469 - 585	7.8e-07	AAA domain (dynein-related subfamily)
MCM_lid	PF17855	650 - 736	3.5e-26	MCM AAA-lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008419)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16440
fragaria_vesca	FvH4_2g09990
malus_domestica	MD00G1085600.v1.1 MD10G1066500.v1.1
prunus_persica	Prupe.8G088400_v2.0.a1
pyrus_communis	pycom05g04970 pycom05g04980 pycom10g05200
rosa_chinensis	RchiOBHm_Chr6g0266881 RchiOBHm_Chr6g0266891
rosa_laevigata	RLG00000014063 RLG00000014064 RLG00000014065
rosa_multiflora	Rmu_co8507775.1_g000001 Rmu_sc0021142.1_g000001 Rmu_sc0036177.1_g000001
rosa_roxburghii	Rroxscaffold_7G00201330
rosa_rugosa	Rorug06G0027900
rosa_samantha	Rh6AG149000 Rh6BG150000 Rh6CG144900 Rh6DG134200
rosa_wichuraiana	Rw6G012890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	651
AasI	GACNNNNNNGTC	2 cut(s)	266, 2301
Acc36I	ACCTGC	1 cut(s)	894
Acc65I	GGTACC	1 cut(s)	552
AccB1I	GGYRCC	1 cut(s)	552
AccII	CGCG	4 cut(s)	157, 208, 213, 1479
AccIII	TCCGGA	1 cut(s)	943
AclWI	GGATC	6 cut(s)	120, 946, 1176, 1418, 1535, 2262
AcoI	YGGCCR	1 cut(s)	220
AcsI	RAATTY	6 cut(s)	143, 388, 874, 1616, 2056, 2271
AcuI	CTGAAG	5 cut(s)	303, 404, 978, 2034, 2411
AcyI	GRCGYC	3 cut(s)	102, 121, 1873
AfaI	GTAC	5 cut(s)	428, 446, 554, 1457, 1923
AfiI	CCNNNNNNNGG	6 cut(s)	114, 562, 962, 1550, 1551, 1849
AgsI	TTSAA	9 cut(s)	31, 626, 718, 744, 853, 1108, 1246, 1369, 1683
AhdI	GACNNNNNGTC	1 cut(s)	1627
AhlI	ACTAGT	1 cut(s)	1490
AjnI	CCWGG	7 cut(s)	869, 933, 1021, 1426, 1817, 2062, 2096
Alw21I	GWGCWC	2 cut(s)	1387, 2405
Alw26I	GTCTC	4 cut(s)	932, 1020, 2287, 2309
AlwI	GGATC	6 cut(s)	120, 946, 1176, 1418, 1535, 2262
Ama87I	CYCGRG	1 cut(s)	560
Aor13HI	TCCGGA	1 cut(s)	943
AoxI	GGCC	6 cut(s)	38, 159, 220, 232, 453, 1333
ApaI	GGGCCC	1 cut(s)	163
ApeKI	GCWGC	7 cut(s)	601, 1447, 1605, 2009, 2212, 2377, 2395
ApoI	RAATTY	6 cut(s)	143, 388, 874, 1616, 2056, 2271
AseI	ATTAAT	1 cut(s)	1404
Asp700I	GAANNNNTTC	1 cut(s)	1212
Asp718I	GGTACC	1 cut(s)	552
AspLEI	GCGC	4 cut(s)	210, 215, 1474, 2367
AspS9I	GGNCC	8 cut(s)	38, 159, 160, 168, 233, 313, 1076, 2354
AsuC2I	CCSGG	1 cut(s)	172
AsuHPI	GGTGA	8 cut(s)	275, 991, 1409, 1433, 1672, 1979, 2092, 2254
AvaI	CYCGRG	1 cut(s)	560
AvaII	GGWCC	4 cut(s)	168, 313, 1076, 2354
BaeGI	GKGCMC	3 cut(s)	163, 206, 338
BanI	GGYRCC	1 cut(s)	552
BanII	GRGCYC	3 cut(s)	71, 163, 2405
BauI	CACGAG	1 cut(s)	782
Bbv12I	GWGCWC	2 cut(s)	1387, 2405
BbvI	GCAGC	7 cut(s)	588, 1434, 1592, 1996, 2224, 2364, 2407
BceAI	ACGGC	3 cut(s)	235, 1496, 2500
BcgI	CGANNNNNNTGC	4 cut(s)	518, 551, 552, 585
BciT130I	CCWGG	7 cut(s)	871, 935, 1023, 1428, 1819, 2064, 2098
BclI	TGATCA	1 cut(s)	2227
BcnI	CCSGG	1 cut(s)	172
BcoDI	GTCTC	4 cut(s)	932, 1020, 2287, 2309
BcuI	ACTAGT	1 cut(s)	1490
BfaI	CTAG	6 cut(s)	738, 1491, 1719, 2025, 2135, 2453
BfoI	RGCGCY	1 cut(s)	1475
BfuAI	ACCTGC	1 cut(s)	894
BglI	GCCNNNNNGGC	1 cut(s)	75
Bme1390I	CCNGG	8 cut(s)	172, 871, 935, 1023, 1428, 1819, 2064, 2098
Bme18I	GGWCC	4 cut(s)	168, 313, 1076, 2354
BmeRI	GACNNNNNGTC	1 cut(s)	1627
BmeT110I	CYCGRG	1 cut(s)	560
BmgT120I	GGNCC	8 cut(s)	38, 159, 160, 168, 233, 313, 1076, 2354
BmiI	GGNNCC	8 cut(s)	161, 170, 554, 932, 1432, 1576, 2355, 2356
BmrFI	CCNGG	8 cut(s)	172, 871, 935, 1023, 1428, 1819, 2064, 2098
BmrI	ACTGGG	1 cut(s)	1053
BmsI	GCATC	8 cut(s)	191, 490, 767, 890, 981, 1000, 2151, 2331
BmuI	ACTGGG	1 cut(s)	1053
BoxI	GACNNNNGTC	1 cut(s)	1727
BplI	GAGNNNNNCTC	2 cut(s)	2289, 2321
BpmI	CTGGAG	3 cut(s)	917, 1436, 2402
Bpu10I	CCTNAGC	1 cut(s)	1689
BpuMI	CCSGG	1 cut(s)	172
Bsa29I	ATCGAT	3 cut(s)	671, 898, 1279
BsaHI	GRCGYC	3 cut(s)	102, 121, 1873
BsaJI	CCNNGG	4 cut(s)	138, 155, 1191, 2096
BsaWI	WCCGGW	2 cut(s)	943, 2237
BsaXI	ACNNNNNCTCC	6 cut(s)	71, 101, 410, 440, 1566, 1596
Bsc4I	CCNNNNNNNGG	6 cut(s)	114, 562, 962, 1550, 1551, 1849
Bse1I	ACTGG	4 cut(s)	1008, 1048, 1453, 2272
Bse3DI	GCAATG	3 cut(s)	514, 1941, 2214
BseAI	TCCGGA	1 cut(s)	943
BseBI	CCWGG	7 cut(s)	871, 935, 1023, 1428, 1819, 2064, 2098
BseCI	ATCGAT	3 cut(s)	671, 898, 1279
BseDI	CCNNGG	4 cut(s)	138, 155, 1191, 2096
BseGI	GGATG	8 cut(s)	182, 313, 403, 633, 662, 711, 952, 2142
BseLI	CCNNNNNNNGG	6 cut(s)	114, 562, 962, 1550, 1551, 1849
BseMI	GCAATG	3 cut(s)	514, 1941, 2214
BseMII	CTCAG	6 cut(s)	825, 1218, 1905, 2166, 2423, 2451
BseNI	ACTGG	4 cut(s)	1008, 1048, 1453, 2272
BseRI	GAGGAG	4 cut(s)	79, 2059, 2062, 2399
BseSI	GKGCMC	3 cut(s)	163, 206, 338
BseXI	GCAGC	7 cut(s)	588, 1434, 1592, 1996, 2224, 2364, 2407
BseYI	CCCAGC	2 cut(s)	162, 1443
Bsh1236I	CGCG	4 cut(s)	157, 208, 213, 1479
Bsh1285I	CGRYCG	2 cut(s)	372, 2247
BshFI	GGCC	6 cut(s)	40, 161, 222, 234, 455, 1335
BshNI	GGYRCC	1 cut(s)	552
BshVI	ATCGAT	3 cut(s)	671, 898, 1279
BsiEI	CGRYCG	2 cut(s)	372, 2247
BsiHKAI	GWGCWC	2 cut(s)	1387, 2405
BsiHKCI	CYCGRG	1 cut(s)	560
BsiSI	CCGG	5 cut(s)	20, 95, 172, 944, 2238
BslFI	GGGAC	5 cut(s)	154, 254, 979, 1426, 2367
BslI	CCNNNNNNNGG	6 cut(s)	114, 562, 962, 1550, 1551, 1849
BsmAI	GTCTC	4 cut(s)	932, 1020, 2287, 2309
BsmBI	CGTCTC	1 cut(s)	2287
BsmFI	GGGAC	5 cut(s)	154, 254, 979, 1426, 2367
BsmI	GAATGC	2 cut(s)	1161, 1366
BsnI	GGCC	6 cut(s)	40, 161, 222, 234, 455, 1335
BsoBI	CYCGRG	1 cut(s)	560
Bsp120I	GGGCCC	1 cut(s)	159
Bsp1286I	GDGCHC	6 cut(s)	71, 163, 206, 338, 1387, 2405
Bsp13I	TCCGGA	1 cut(s)	943
Bsp1407I	TGTACA	1 cut(s)	1921
BspANI	GGCC	6 cut(s)	40, 161, 222, 234, 455, 1335
BspCNI	CTCAG	6 cut(s)	826, 1217, 1906, 2165, 2424, 2452
BspDI	ATCGAT	3 cut(s)	671, 898, 1279
BspEI	TCCGGA	1 cut(s)	943
BspFNI	CGCG	4 cut(s)	157, 208, 213, 1479
BspLI	GGNNCC	8 cut(s)	161, 170, 554, 932, 1432, 1576, 2355, 2356
BspMI	ACCTGC	1 cut(s)	894
BspPI	GGATC	6 cut(s)	120, 946, 1176, 1418, 1535, 2262
BspT107I	GGYRCC	1 cut(s)	552
BsrDI	GCAATG	3 cut(s)	514, 1941, 2214
BsrGI	TGTACA	1 cut(s)	1921
BsrI	ACTGG	4 cut(s)	1008, 1048, 1453, 2272
BssECI	CCNNGG	4 cut(s)	138, 155, 1191, 2096
BssNI	GRCGYC	3 cut(s)	102, 121, 1873
BssSI	CACGAG	1 cut(s)	782
BssT1I	CCWWGG	1 cut(s)	1191
Bst2BI	CACGAG	1 cut(s)	782
Bst2UI	CCWGG	7 cut(s)	871, 935, 1023, 1428, 1819, 2064, 2098
Bst4CI	ACNGT	8 cut(s)	263, 979, 1066, 1093, 1104, 1561, 1678, 2492
Bst6I	CTCTTC	5 cut(s)	813, 852, 1203, 1492, 2010
BstACI	GRCGYC	3 cut(s)	102, 121, 1873
BstAPI	GCANNNNNTGC	1 cut(s)	1605
BstAUI	TGTACA	1 cut(s)	1921
BstDSI	CCRYGG	1 cut(s)	155
BstENI	CCTNNNNNAGG	2 cut(s)	960, 1847
BstF5I	GGATG	8 cut(s)	182, 313, 403, 633, 662, 711, 952, 2142
BstFNI	CGCG	4 cut(s)	157, 208, 213, 1479
BstH2I	RGCGCY	1 cut(s)	1475
BstHHI	GCGC	4 cut(s)	210, 215, 1474, 2367
BstMAI	GTCTC	4 cut(s)	932, 1020, 2287, 2309
BstMCI	CGRYCG	2 cut(s)	372, 2247
BstNI	CCWGG	7 cut(s)	871, 935, 1023, 1428, 1819, 2064, 2098
BstNSI	RCATGY	1 cut(s)	1651
BstPAI	GACNNNNGTC	1 cut(s)	1727
BstSCI	CCNGG	8 cut(s)	170, 869, 933, 1021, 1426, 1817, 2062, 2096
BstSLI	GKGCMC	3 cut(s)	163, 206, 338
BstUI	CGCG	4 cut(s)	157, 208, 213, 1479
BstV1I	GCAGC	7 cut(s)	588, 1434, 1592, 1996, 2224, 2364, 2407
BstX2I	RGATCY	3 cut(s)	951, 1540, 2254
BstXI	CCANNNNNNTGG	3 cut(s)	230, 1308, 1352
BstYI	RGATCY	3 cut(s)	951, 1540, 2254
Bsu15I	ATCGAT	3 cut(s)	671, 898, 1279
BsuRI	GGCC	6 cut(s)	40, 161, 222, 234, 455, 1335
BsuTUI	ATCGAT	3 cut(s)	671, 898, 1279
BtgI	CCRYGG	1 cut(s)	155
BtsCI	GGATG	8 cut(s)	182, 313, 403, 633, 662, 711, 952, 2142
BtsI	GCAGTG	1 cut(s)	413
BtsIMutI	CAGTG	4 cut(s)	413, 1100, 2265, 2497
BveI	ACCTGC	1 cut(s)	894
CfoI	GCGC	4 cut(s)	210, 215, 1474, 2367
Cfr13I	GGNCC	8 cut(s)	38, 159, 160, 168, 233, 313, 1076, 2354
Cfr42I	CCGCGG	1 cut(s)	158
ClaI	ATCGAT	3 cut(s)	671, 898, 1279
CseI	GACGC	3 cut(s)	110, 110, 1881
Csp6I	GTAC	5 cut(s)	427, 445, 553, 1456, 1922
CviAII	CATG	7 cut(s)	304, 880, 995, 1648, 1655, 2171, 2251
CviQI	GTAC	5 cut(s)	427, 445, 553, 1456, 1922
DrdI	GACNNNNNNGTC	2 cut(s)	266, 2301
DriI	GACNNNNNGTC	1 cut(s)	1627
DseDI	GACNNNNNNGTC	2 cut(s)	266, 2301
EaeI	YGGCCR	1 cut(s)	220
Eam1104I	CTCTTC	5 cut(s)	813, 852, 1203, 1492, 2010
Eam1105I	GACNNNNNGTC	1 cut(s)	1627
EarI	CTCTTC	5 cut(s)	813, 852, 1203, 1492, 2010
EciI	GGCGGA	3 cut(s)	38, 113, 1178
Ecl136II	GAGCTC	1 cut(s)	2403
Eco130I	CCWWGG	1 cut(s)	1191
Eco147I	AGGCCT	1 cut(s)	1335
Eco24I	GRGCYC	3 cut(s)	71, 163, 2405
Eco47I	GGWCC	4 cut(s)	168, 313, 1076, 2354
Eco53kI	GAGCTC	1 cut(s)	2403
Eco57I	CTGAAG	5 cut(s)	303, 404, 978, 2034, 2411
Eco88I	CYCGRG	1 cut(s)	560
EcoICRI	GAGCTC	1 cut(s)	2403
EcoNI	CCTNNNNNAGG	2 cut(s)	960, 1847
EcoO109I	RGGNCCY	1 cut(s)	2354
EcoRI	GAATTC	1 cut(s)	874
EcoRII	CCWGG	7 cut(s)	869, 933, 1021, 1426, 1817, 2062, 2096
EcoT14I	CCWWGG	1 cut(s)	1191
EcoT22I	ATGCAT	1 cut(s)	996
EcoT38I	GRGCYC	3 cut(s)	71, 163, 2405
ErhI	CCWWGG	1 cut(s)	1191
Esp3I	CGTCTC	1 cut(s)	2287
FaeI	CATG	7 cut(s)	307, 883, 998, 1651, 1658, 2174, 2254
FalI	AAGNNNNNCTT	4 cut(s)	1200, 1232, 1323, 1355
FaqI	GGGAC	5 cut(s)	154, 254, 979, 1426, 2367
FatI	CATG	7 cut(s)	303, 879, 994, 1647, 1654, 2170, 2250
FauI	CCCGC	3 cut(s)	150, 162, 279
FauNDI	CATATG	1 cut(s)	1960
FbaI	TGATCA	1 cut(s)	2227
FokI	GGATG	8 cut(s)	169, 320, 410, 620, 649, 718, 959, 2129
FriOI	GRGCYC	3 cut(s)	71, 163, 2405
FspBI	CTAG	6 cut(s)	738, 1491, 1719, 2025, 2135, 2453
GlaI	GCGC	4 cut(s)	209, 214, 1473, 2366
GsaI	CCCAGC	2 cut(s)	166, 1447
GsuI	CTGGAG	3 cut(s)	917, 1436, 2402
HaeII	RGCGCY	1 cut(s)	1475
HaeIII	GGCC	6 cut(s)	40, 161, 222, 234, 455, 1335
HapII	CCGG	5 cut(s)	20, 95, 172, 944, 2238
HgaI	GACGC	3 cut(s)	110, 110, 1881
HhaI	GCGC	4 cut(s)	210, 215, 1474, 2367
Hin1I	GRCGYC	3 cut(s)	102, 121, 1873
Hin1II	CATG	7 cut(s)	307, 883, 998, 1651, 1658, 2174, 2254
Hin6I	GCGC	4 cut(s)	208, 213, 1472, 2365
HinP1I	GCGC	4 cut(s)	208, 213, 1472, 2365
HincII	GTYRAC	3 cut(s)	1183, 1953, 2022
HindII	GTYRAC	3 cut(s)	1183, 1953, 2022
HindIII	AAGCTT	1 cut(s)	1370
HinfI	GANTC	9 cut(s)	11, 56, 401, 485, 524, 940, 1213, 1909, 2156
HpaII	CCGG	5 cut(s)	20, 95, 172, 944, 2238
HphI	GGTGA	8 cut(s)	275, 991, 1409, 1433, 1672, 1979, 2092, 2254
Hpy166II	GTNNAC	8 cut(s)	352, 909, 1183, 1757, 1922, 1953, 2022, 2482
Hpy188III	TCNNGA	9 cut(s)	50, 395, 460, 944, 1108, 1152, 1544, 1913, 2198
Hpy8I	GTNNAC	8 cut(s)	352, 909, 1183, 1757, 1922, 1953, 2022, 2482
Hpy99I	CGWCG	4 cut(s)	126, 263, 296, 359
HpyCH4III	ACNGT	8 cut(s)	263, 979, 1066, 1093, 1104, 1561, 1678, 2492
HpyCH4IV	ACGT	2 cut(s)	357, 1949
HpySE526I	ACGT	2 cut(s)	357, 1949
Hsp92I	GRCGYC	3 cut(s)	102, 121, 1873
Hsp92II	CATG	7 cut(s)	307, 883, 998, 1651, 1658, 2174, 2254
HspAI	GCGC	4 cut(s)	208, 213, 1472, 2365
KflI	GGGWCCC	1 cut(s)	2354
Kpn2I	TCCGGA	1 cut(s)	943
KpnI	GGTACC	1 cut(s)	556
Ksp22I	TGATCA	1 cut(s)	2227
KspI	CCGCGG	1 cut(s)	158
LmnI	GCTCC	5 cut(s)	936, 1455, 1574, 1644, 2386
Lsp1109I	GCAGC	7 cut(s)	588, 1434, 1592, 1996, 2224, 2364, 2407
LweI	GCATC	8 cut(s)	191, 490, 767, 890, 981, 1000, 2151, 2331
MaeI	CTAG	6 cut(s)	738, 1491, 1719, 2025, 2135, 2453
MaeII	ACGT	2 cut(s)	357, 1949
MaeIII	GTNAC	1 cut(s)	1039
MfeI	CAATTG	1 cut(s)	1241
MflI	RGATCY	3 cut(s)	951, 1540, 2254
MhlI	GDGCHC	6 cut(s)	71, 163, 206, 338, 1387, 2405
MlyI	GAGTC	3 cut(s)	50, 410, 934
MmeI	TCCRAC	5 cut(s)	552, 825, 1054, 1177, 1495
Mph1103I	ATGCAT	1 cut(s)	996
MroI	TCCGGA	1 cut(s)	943
MroXI	GAANNNNTTC	1 cut(s)	1212
MseI	TTAA	4 cut(s)	360, 1283, 1404, 1713
MslI	CAYNNNNRTG	3 cut(s)	671, 2330, 2424
MspA1I	CMGCKG	3 cut(s)	157, 166, 1447
MspI	CCGG	5 cut(s)	20, 95, 172, 944, 2238
MspR9I	CCNGG	8 cut(s)	172, 871, 935, 1023, 1428, 1819, 2064, 2098
MteI	GCGCNGCGC	1 cut(s)	211
MunI	CAATTG	1 cut(s)	1241
Mva1269I	GAATGC	2 cut(s)	1161, 1366
MvaI	CCWGG	7 cut(s)	871, 935, 1023, 1428, 1819, 2064, 2098
MvnI	CGCG	4 cut(s)	157, 208, 213, 1479
NciI	CCSGG	1 cut(s)	172
NdeI	CATATG	1 cut(s)	1960
NlaIII	CATG	7 cut(s)	307, 883, 998, 1651, 1658, 2174, 2254
NlaIV	GGNNCC	8 cut(s)	161, 170, 554, 932, 1432, 1576, 2355, 2356
NsiI	ATGCAT	1 cut(s)	996
NspI	RCATGY	1 cut(s)	1651
PaeI	GCATGC	1 cut(s)	1651
PaeR7I	CTCGAG	1 cut(s)	560
PceI	AGGCCT	1 cut(s)	1335
PcsI	WCGNNNNNNNCGW	1 cut(s)	183
PctI	GAATGC	2 cut(s)	1161, 1366
PdmI	GAANNNNTTC	1 cut(s)	1212
PfeI	GAWTC	6 cut(s)	11, 485, 524, 1213, 1909, 2156
PflFI	GACNNNGTC	1 cut(s)	2492
PfoI	TCCNGGA	2 cut(s)	933, 1426
Ple19I	CGATCG	2 cut(s)	372, 2247
PleI	GAGTC	3 cut(s)	50, 409, 934
PpsI	GAGTC	3 cut(s)	50, 409, 934
PpuMI	RGGWCCY	1 cut(s)	2354
PshAI	GACNNNNGTC	1 cut(s)	1727
PshBI	ATTAAT	1 cut(s)	1404
PsiI	TTATAA	1 cut(s)	651
Psp124BI	GAGCTC	1 cut(s)	2405
Psp5II	RGGWCCY	1 cut(s)	2354
Psp6I	CCWGG	7 cut(s)	869, 933, 1021, 1426, 1817, 2062, 2096
PspFI	CCCAGC	2 cut(s)	162, 1443
PspGI	CCWGG	7 cut(s)	869, 933, 1021, 1426, 1817, 2062, 2096
PspN4I	GGNNCC	8 cut(s)	161, 170, 554, 932, 1432, 1576, 2355, 2356
PspOMI	GGGCCC	1 cut(s)	159
PspPI	GGNCC	8 cut(s)	38, 159, 160, 168, 233, 313, 1076, 2354
PspPPI	RGGWCCY	1 cut(s)	2354
PspXI	VCTCGAGB	1 cut(s)	560
PsuI	RGATCY	3 cut(s)	951, 1540, 2254
PsyI	GACNNNGTC	1 cut(s)	2492
PvuI	CGATCG	2 cut(s)	372, 2247
PvuII	CAGCTG	1 cut(s)	1447
RsaI	GTAC	5 cut(s)	428, 446, 554, 1457, 1923
RsaNI	GTAC	5 cut(s)	427, 445, 553, 1456, 1922
RseI	CAYNNNNRTG	3 cut(s)	671, 2330, 2424
SacI	GAGCTC	1 cut(s)	2405
SacII	CCGCGG	1 cut(s)	158
SaqAI	TTAA	4 cut(s)	360, 1283, 1404, 1713
Sau96I	GGNCC	8 cut(s)	38, 159, 160, 168, 233, 313, 1076, 2354
SchI	GAGTC	3 cut(s)	50, 410, 934
ScrFI	CCNGG	8 cut(s)	172, 871, 935, 1023, 1428, 1819, 2064, 2098
SduI	GDGCHC	6 cut(s)	71, 163, 206, 338, 1387, 2405
SfaNI	GCATC	8 cut(s)	191, 490, 767, 890, 981, 1000, 2151, 2331
Sfr274I	CTCGAG	1 cut(s)	560
Sfr303I	CCGCGG	1 cut(s)	158
SgrBI	CCGCGG	1 cut(s)	158
SinI	GGWCC	4 cut(s)	168, 313, 1076, 2354
SlaI	CTCGAG	1 cut(s)	560
SmiMI	CAYNNNNRTG	3 cut(s)	671, 2330, 2424
SmlI	CTYRAG	1 cut(s)	560
SmoI	CTYRAG	1 cut(s)	560
SpeI	ACTAGT	1 cut(s)	1490
SphI	GCATGC	1 cut(s)	1651
SseBI	AGGCCT	1 cut(s)	1335
SspI	AATATT	2 cut(s)	771, 1408
SspMI	CTAG	6 cut(s)	738, 1491, 1719, 2025, 2135, 2453
SstI	GAGCTC	1 cut(s)	2405
StuI	AGGCCT	1 cut(s)	1335
StyD4I	CCNGG	8 cut(s)	170, 869, 933, 1021, 1426, 1817, 2062, 2096
StyI	CCWWGG	1 cut(s)	1191
TaaI	ACNGT	8 cut(s)	263, 979, 1066, 1093, 1104, 1561, 1678, 2492
TaiI	ACGT	2 cut(s)	360, 1952
TaqI	TCGA	8 cut(s)	65, 124, 561, 671, 775, 898, 1279, 2247
TaqII	GACCGA	3 cut(s)	330, 723, 1717
TatI	WGTACW	3 cut(s)	444, 1455, 1921
TauI	GCSGC	4 cut(s)	182, 213, 291, 2412
TfiI	GAWTC	6 cut(s)	11, 485, 524, 1213, 1909, 2156
Tru1I	TTAA	4 cut(s)	360, 1283, 1404, 1713
Tru9I	TTAA	4 cut(s)	360, 1283, 1404, 1713
TscAI	CASTG	4 cut(s)	413, 1107, 2272, 2497
TseI	GCWGC	7 cut(s)	601, 1447, 1605, 2009, 2212, 2377, 2395
TspDTI	ATGAA	2 cut(s)	881, 1239
TspGWI	ACGGA	3 cut(s)	431, 926, 2135
TspRI	CASTG	4 cut(s)	413, 1107, 2272, 2497
Tth111I	GACNNNGTC	1 cut(s)	2492
VpaK11BI	GGWCC	4 cut(s)	168, 313, 1076, 2354
VspI	ATTAAT	1 cut(s)	1404
XagI	CCTNNNNNAGG	2 cut(s)	960, 1847
XapI	RAATTY	6 cut(s)	143, 388, 874, 1616, 2056, 2271
XceI	RCATGY	1 cut(s)	1651
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1352
XhoI	CTCGAG	1 cut(s)	560
XmnI	GAANNNNTTC	1 cut(s)	1212
XspI	CTAG	6 cut(s)	738, 1491, 1719, 2025, 2135, 2453
Zsp2I	ATGCAT	1 cut(s)	996

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.