Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0008419

Physical Location & Seq

Genomic Coordinates Forward (+)

24122503 .. 24128927

Gene Structure 6425 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG150000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2505 bp

ATGGCTTCCGACTCTTCTGCACCCAATTTCAACAACGGGCCTTCCTCTCCCGATGACTCCTTCTCCAGCCCAATCGGCAACACCTTCTCTTCTCCGGCCGACGCCGCCGCCGCTCGCCAGAGAAGCCGCCGCAGATCCACCACACCCACCGCATTCGCAACCCCTCCCGCCTCCCGCTCCCGCTTCGCCGCCTCCGAAACAACCCCAACCCCATCATCTCGCACGCGCCGCCCCAACGGCACCGCACGCACCCCCTCCTCTACCGATGATGGGCCCCCCTCTTCGGAAGGCGGCGACAGCTACGGCATGGACGAGGACCGCCCCACCTTCGTCTGGGGCACCAATATCAGCGTCAATGATGTTAAGCTCGCCATTATTAGGTTTTTGAAGAATTTTCGGGACCAATCGCAGAACTCCGAGAGCGATTATCTTACCGAAGGGAAATACATTGAGGCCATCAAGAGAATTCTGGAAATGGAAGGCGATTCCCTGGATGTGGATGCCCGCAATGTGTTTGATTATGATTCCGATTTGTATGCTAAGATGGTCAGGTACCCACTTGAGGTTCTTGCTATTTTCGACATTGTTTTGATGGAATTGTTGCCTGTCATAAACCCCTTGTTCGAGAAGCACATTCAGACCCGCATTTTCAATCTCAAAACATCAACATCCATGAGAAATCTGAACCCGTCTGATATTGAGAGGATGGTTTCAATAAAGGGGATGATTATTCGGTCCAGTTCAATAATACCTGAGATAAGGGAAGCAATATTTAGATGCCTCGTGTGTGGTTACTACTCTGACCCTCTTCCTGTAGAAAAAGGGAAAATAAATGAACCTACAAGATGCATGAAGGAAGAGTGTCAAGCCAAGAATTCTATGACACTTGTTCACAACCGTTGCAGGTTTACTGATAAACAGATTGTGAGGCTCCAGGAGACACCTGATGAGATCCCTGAAGGAGGGACACCGCATACTGTGAGTTTGTTGATGCATGACAAACTGGTTGATGCGGGAAAACCAGGTGACAGAGTTGAGGTTACTGGGATATATCGGGCTATGAGCGTAAGAGTTGGACCTACACAGAGAACTGTAAAATCATTATTCAAGACTTATATTGATTGTCTGCATATAAAAAAGTCTGACAAGTCAAGAATGCTGGCGGAGGAGCAGACGGAAGTTGAGAATTCCTTGGGTGGAAATTCAGATGAGATTCTTTTTGATGAAAAGAAGGTGGAACAATTGAAAGAGTTGGCAAAACAGCCTGATATATATGATCGCCTAACCAGATCTTTGGCACCAAACATCTGGGAGTTAGATGATGTCAAGAAAGGCCTTCTTTGCCAGCTTTTTGGTGGGAATGCTTTGAAGTTGCCATCTGGTGCAAGGTTCCGTGGGGATATTAATATTCTTCTTGTTGGTGATCCTGGAACCAGCAAGTCCCAGCTGCTCCAGTACATACACAAGCTTGCTCCCCGTGGCATATACACTAGTGGAAGAGGGAGTTCTGCTGTTGGTTTGACTGCTTATGTTGCAAAAGATCCTGAAACAGGGGAAACTGTTTTAGAGAGTGGAGCCTTAGTTTTGAGTGATCGAGGCATTTGTTGCATTGATGAATTTGACAAAATGTCTGACAGTGCAAGGAGCATGTTACACGAGGTGATGGAGCAGCAAACTGTGTCAATAGCAAAGGCGGGAATCATTGCTTCCCTTAATGCTAGGACATCAGTCCTGGCTTGTGCAAATCCAAGTGGATCTCGCTATAATCCTCGCTTGTCAGTAATTGACAACATACACCTTCCTCCTACCTTATTATCCAGGTTTGATCTGATCTACTTAATCCTTGACAAGGCTGATGAGCAGACAGACAGGCGCCTTGCAAAGCATATTGTTGCCTTGCACTTTGAGAATCCTGAGACTGTACAGCAGGATGTTTTAGACCTTGCCACGTTGACTGCTTATGTGGGATATGCCCGAAAGCATATTCACCCACAATTATCTGATGAAGCTGCAGAGGAGTTGACTCGTGGCTATGTAGAGTTGAGGAGGAGAGGAAATTTTCCGGGCAGCAGCAAAAAGGTGATAACAGCAACTCCTAGGCAAATCGAGAGTTTGATACGTCTTAGTGAAGCTCTAGCTCGGATTCGTTTTTCAGAATCGGTTGAAAAGCATGATGTAATGGAGGCATTTCGGCTTCTTGAAGTTGCAATGCAACAATCAGCAACAGATCACTCCACTGGAACGATCGACATGGATCTTATTAATACTGGAATTTCGGCAAGTGAAAGAATGAGACGGGAGAATCTAGTGTCATCAGCTCGCAACTTAATTATGGAGAAGATGCAACTTGGGGGACCCTCAATGCGTTTGTTAGAGCTGCTGGAGGAGCTGAAGAAGCAGAGTTCTGGCAGTGAAATTCACCTCAACGATCTTAGGAATGCAATTGCTACGCTTGCAAGTGAGGGATTTGTCGCTGTTCATGGAGACGGTGTCAAAAGAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0000785 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

834

Amino Acids

92.52

Weight (kDa)

6.14

Isoelectric Point (pI)

50.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	125 - 217	1.2e-11	MCM N-terminal domain
MCM_OB	PF17207	225 - 354	2.4e-40	MCM OB domain
MCM	PF00493	412 - 634	3.8e-101	MCM P-loop domain
AAA_5	PF07728	469 - 585	7.9e-07	AAA domain (dynein-related subfamily)
MCM_lid	PF17855	650 - 736	1.1e-26	MCM AAA-lid domain
WHD_MCM4	PF21128	760 - 834	1.6e-07	MCM4, winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008419)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16440
fragaria_vesca	FvH4_2g09990
malus_domestica	MD00G1085600.v1.1 MD10G1066500.v1.1
prunus_persica	Prupe.8G088400_v2.0.a1
pyrus_communis	pycom05g04970 pycom05g04980 pycom10g05200
rosa_chinensis	RchiOBHm_Chr6g0266881 RchiOBHm_Chr6g0266891
rosa_laevigata	RLG00000014063 RLG00000014064 RLG00000014065
rosa_multiflora	Rmu_co8507775.1_g000001 Rmu_sc0021142.1_g000001 Rmu_sc0036177.1_g000001
rosa_roxburghii	Rroxscaffold_7G00201330
rosa_rugosa	Rorug06G0027900
rosa_samantha	Rh6AG149000 Rh6BG150000 Rh6CG144900 Rh6DG134200
rosa_wichuraiana	Rw6G012890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	894
Acc65I	GGTACC	1 cut(s)	552
AccB1I	GGYRCC	5 cut(s)	239, 338, 552, 1297, 1872
AccBSI	CCGCTC	2 cut(s)	113, 177
AccII	CGCG	1 cut(s)	226
AclWI	GGATC	6 cut(s)	129, 946, 1418, 1535, 1762, 2262
AcoI	YGGCCR	1 cut(s)	96
AcsI	RAATTY	9 cut(s)	391, 465, 874, 1186, 1201, 1616, 2056, 2271, 2415
AcuI	CTGAAG	2 cut(s)	978, 2411
AcyI	GRCGYC	2 cut(s)	102, 1873
AdeI	CACNNNGTG	1 cut(s)	1660
AfaI	GTAC	3 cut(s)	554, 1457, 1923
AfiI	CCNNNNNNNGG	9 cut(s)	270, 283, 333, 378, 496, 562, 962, 1550, 1849
AhdI	GACNNNNNGTC	1 cut(s)	1627
AhlI	ACTAGT	1 cut(s)	1490
AjnI	CCWGG	6 cut(s)	489, 933, 1021, 1426, 1731, 1817
Alw26I	GTCTC	4 cut(s)	932, 1910, 2287, 2478
AlwI	GGATC	6 cut(s)	129, 946, 1418, 1535, 1762, 2262
AoxI	GGCC	5 cut(s)	38, 96, 272, 453, 1333
ApaI	GGGCCC	1 cut(s)	276
ApeKI	GCWGC	6 cut(s)	1447, 1669, 2009, 2067, 2070, 2377
ApoI	RAATTY	9 cut(s)	391, 465, 874, 1186, 1201, 1616, 2056, 2271, 2415
AseI	ATTAAT	2 cut(s)	1404, 2262
Asp718I	GGTACC	1 cut(s)	552
AspA2I	CCTAGG	1 cut(s)	2096
AspLEI	GCGC	2 cut(s)	228, 1875
AspS9I	GGNCC	8 cut(s)	38, 272, 273, 316, 400, 735, 1076, 2354
AsuC2I	CCSGG	1 cut(s)	2064
AsuHPI	GGTGA	6 cut(s)	1037, 1433, 1672, 1979, 2092, 2411
AvaII	GGWCC	5 cut(s)	316, 400, 735, 1076, 2354
AvrII	CCTAGG	1 cut(s)	2096
BaeGI	GKGCMC	2 cut(s)	276, 341
BanI	GGYRCC	5 cut(s)	239, 338, 552, 1297, 1872
BanII	GRGCYC	1 cut(s)	276
BauI	CACGAG	3 cut(s)	782, 1655, 2025
BbvI	GCAGC	6 cut(s)	1434, 1681, 1996, 2079, 2082, 2364
BccI	CCATC	8 cut(s)	220, 263, 464, 538, 586, 700, 1384, 1657
BceAI	ACGGC	2 cut(s)	253, 319
BcgI	CGANNNNNNTGC	3 cut(s)	33, 518, 552
BciT130I	CCWGG	6 cut(s)	491, 935, 1023, 1428, 1733, 1819
BcnI	CCSGG	1 cut(s)	2064
BcoDI	GTCTC	4 cut(s)	932, 1910, 2287, 2478
BcuI	ACTAGT	1 cut(s)	1490
BfaI	CTAG	5 cut(s)	1491, 1719, 2097, 2135, 2306
BfmI	CTRYAG	2 cut(s)	813, 2010
BfoI	RGCGCY	1 cut(s)	1876
BfuAI	ACCTGC	1 cut(s)	894
BglI	GCCNNNNNGGC	2 cut(s)	75, 237
BglII	AGATCT	1 cut(s)	1289
BlnI	CCTAGG	1 cut(s)	2096
Bme1390I	CCNGG	7 cut(s)	491, 935, 1023, 1428, 1733, 1819, 2064
Bme18I	GGWCC	5 cut(s)	316, 400, 735, 1076, 2354
BmeRI	GACNNNNNGTC	1 cut(s)	1627
BmgT120I	GGNCC	8 cut(s)	38, 272, 273, 316, 400, 735, 1076, 2354
BmrFI	CCNGG	7 cut(s)	491, 935, 1023, 1428, 1733, 1819, 2064
BmrI	ACTGGG	1 cut(s)	1053
BmsI	GCATC	6 cut(s)	490, 767, 836, 981, 1000, 2331
BmuI	ACTGGG	1 cut(s)	1053
BoxI	GACNNNNGTC	1 cut(s)	1727
BpmI	CTGGAG	4 cut(s)	49, 917, 1436, 2402
BpuEI	CTTGAG	1 cut(s)	581
BpuMI	CCSGG	1 cut(s)	2064
BsaHI	GRCGYC	2 cut(s)	102, 1873
BsaJI	CCNNGG	5 cut(s)	489, 1191, 1393, 1477, 2096
BsaXI	ACNNNNNCTCC	8 cut(s)	47, 77, 1566, 1596, 2077, 2107, 2475, 2505
Bsc4I	CCNNNNNNNGG	9 cut(s)	270, 283, 333, 378, 496, 562, 962, 1550, 1849
Bse1I	ACTGG	6 cut(s)	738, 1008, 1048, 1453, 2242, 2272
Bse3DI	GCAATG	3 cut(s)	514, 1701, 2214
BseBI	CCWGG	6 cut(s)	491, 935, 1023, 1428, 1733, 1819
BseDI	CCNNGG	5 cut(s)	489, 1191, 1393, 1477, 2096
BseGI	GGATG	6 cut(s)	499, 505, 668, 711, 729, 1936
BseLI	CCNNNNNNNGG	9 cut(s)	270, 283, 333, 378, 496, 562, 962, 1550, 1849
BseMI	GCAATG	3 cut(s)	514, 1701, 2214
BseMII	CTCAG	2 cut(s)	744, 1905
BseNI	ACTGG	6 cut(s)	738, 1008, 1048, 1453, 2242, 2272
BseRI	GAGGAG	6 cut(s)	247, 1181, 2030, 2059, 2062, 2399
BseSI	GKGCMC	2 cut(s)	276, 341
BseX3I	CGGCCG	1 cut(s)	96
BseXI	GCAGC	6 cut(s)	1434, 1681, 1996, 2079, 2082, 2364
BseYI	CCCAGC	1 cut(s)	1443
Bsh1236I	CGCG	1 cut(s)	226
Bsh1285I	CGRYCG	2 cut(s)	99, 2247
BshFI	GGCC	5 cut(s)	40, 98, 274, 455, 1335
BshNI	GGYRCC	5 cut(s)	239, 338, 552, 1297, 1872
BsiEI	CGRYCG	2 cut(s)	99, 2247
BsiSI	CCGG	2 cut(s)	95, 2063
BslFI	GGGAC	4 cut(s)	413, 979, 1426, 2367
BslI	CCNNNNNNNGG	9 cut(s)	270, 283, 333, 378, 496, 562, 962, 1550, 1849
BsmAI	GTCTC	4 cut(s)	932, 1910, 2287, 2478
BsmBI	CGTCTC	2 cut(s)	2287, 2478
BsmFI	GGGAC	4 cut(s)	413, 979, 1426, 2367
BsmI	GAATGC	4 cut(s)	152, 1161, 1366, 2443
BsnI	GGCC	5 cut(s)	40, 98, 274, 455, 1335
Bsp120I	GGGCCC	1 cut(s)	272
Bsp1286I	GDGCHC	2 cut(s)	276, 341
Bsp1407I	TGTACA	1 cut(s)	1921
BspANI	GGCC	5 cut(s)	40, 98, 274, 455, 1335
BspCNI	CTCAG	2 cut(s)	745, 1906
BspFNI	CGCG	1 cut(s)	226
BspMAI	CTGCAG	1 cut(s)	2014
BspMI	ACCTGC	1 cut(s)	894
BspPI	GGATC	6 cut(s)	129, 946, 1418, 1535, 1762, 2262
BspT107I	GGYRCC	5 cut(s)	239, 338, 552, 1297, 1872
BsrBI	CCGCTC	2 cut(s)	113, 177
BsrDI	GCAATG	3 cut(s)	514, 1701, 2214
BsrGI	TGTACA	1 cut(s)	1921
BsrI	ACTGG	6 cut(s)	738, 1008, 1048, 1453, 2242, 2272
BssECI	CCNNGG	5 cut(s)	489, 1191, 1393, 1477, 2096
BssNI	GRCGYC	2 cut(s)	102, 1873
BssSI	CACGAG	3 cut(s)	782, 1655, 2025
BssT1I	CCWWGG	2 cut(s)	1191, 2096
Bst2BI	CACGAG	3 cut(s)	782, 1655, 2025
Bst2UI	CCWGG	6 cut(s)	491, 935, 1023, 1428, 1733, 1819
Bst4CI	ACNGT	8 cut(s)	899, 979, 1093, 1561, 1637, 1678, 1921, 2489
Bst6I	CTCTTC	6 cut(s)	19, 94, 286, 813, 852, 1492
BstACI	GRCGYC	2 cut(s)	102, 1873
BstAPI	GCANNNNNTGC	1 cut(s)	1605
BstAUI	TGTACA	1 cut(s)	1921
BstDEI	CTNAG	6 cut(s)	540, 753, 1579, 1914, 2123, 2432
BstDSI	CCRYGG	2 cut(s)	1393, 1477
BstENI	CCTNNNNNAGG	3 cut(s)	960, 1548, 1847
BstF5I	GGATG	6 cut(s)	499, 505, 668, 711, 729, 1936
BstFNI	CGCG	1 cut(s)	226
BstH2I	RGCGCY	1 cut(s)	1876
BstHHI	GCGC	2 cut(s)	228, 1875
BstMAI	GTCTC	4 cut(s)	932, 1910, 2287, 2478
BstMCI	CGRYCG	2 cut(s)	99, 2247
BstNI	CCWGG	6 cut(s)	491, 935, 1023, 1428, 1733, 1819
BstNSI	RCATGY	1 cut(s)	1651
BstPAI	GACNNNNGTC	1 cut(s)	1727
BstSCI	CCNGG	7 cut(s)	489, 933, 1021, 1426, 1731, 1817, 2062
BstSFI	CTRYAG	2 cut(s)	813, 2010
BstSLI	GKGCMC	2 cut(s)	276, 341
BstUI	CGCG	1 cut(s)	226
BstV1I	GCAGC	6 cut(s)	1434, 1681, 1996, 2079, 2082, 2364
BstX2I	RGATCY	6 cut(s)	134, 951, 1289, 1540, 1754, 2254
BstXI	CCANNNNNNTGG	3 cut(s)	1294, 1308, 1352
BstYI	RGATCY	6 cut(s)	134, 951, 1289, 1540, 1754, 2254
BstZI	CGGCCG	1 cut(s)	96
BsuRI	GGCC	5 cut(s)	40, 98, 274, 455, 1335
BtgI	CCRYGG	2 cut(s)	1393, 1477
BtsCI	GGATG	6 cut(s)	499, 505, 668, 711, 729, 1936
BtsI	GCAGTG	1 cut(s)	2416
BtsIMutI	CAGTG	3 cut(s)	1642, 2235, 2416
BveI	ACCTGC	1 cut(s)	894
CfoI	GCGC	2 cut(s)	228, 1875
Cfr13I	GGNCC	8 cut(s)	38, 272, 273, 316, 400, 735, 1076, 2354
CseI	GACGC	2 cut(s)	110, 340
CsiI	ACCWGGT	1 cut(s)	1021
Csp6I	GTAC	3 cut(s)	553, 1456, 1922
CviAII	CATG	8 cut(s)	307, 673, 850, 995, 1648, 2171, 2251, 2480
CviQI	GTAC	3 cut(s)	553, 1456, 1922
DdeI	CTNAG	6 cut(s)	540, 753, 1579, 1914, 2123, 2432
DinI	GGCGCC	1 cut(s)	1874
DraIII	CACNNNGTG	1 cut(s)	1660
DriI	GACNNNNNGTC	1 cut(s)	1627
EaeI	YGGCCR	1 cut(s)	96
EagI	CGGCCG	1 cut(s)	96
Eam1104I	CTCTTC	6 cut(s)	19, 94, 286, 813, 852, 1492
Eam1105I	GACNNNNNGTC	1 cut(s)	1627
EarI	CTCTTC	6 cut(s)	19, 94, 286, 813, 852, 1492
EciI	GGCGGA	1 cut(s)	1178
EclXI	CGGCCG	1 cut(s)	96
Eco130I	CCWWGG	2 cut(s)	1191, 2096
Eco147I	AGGCCT	1 cut(s)	1335
Eco24I	GRGCYC	1 cut(s)	276
Eco47I	GGWCC	5 cut(s)	316, 400, 735, 1076, 2354
Eco52I	CGGCCG	1 cut(s)	96
Eco57I	CTGAAG	2 cut(s)	978, 2411
EcoNI	CCTNNNNNAGG	3 cut(s)	960, 1548, 1847
EcoO109I	RGGNCCY	2 cut(s)	273, 2354
EcoRI	GAATTC	3 cut(s)	465, 874, 1186
EcoRII	CCWGG	6 cut(s)	489, 933, 1021, 1426, 1731, 1817
EcoT14I	CCWWGG	2 cut(s)	1191, 2096
EcoT22I	ATGCAT	2 cut(s)	851, 996
EcoT38I	GRGCYC	1 cut(s)	276
EgeI	GGCGCC	1 cut(s)	1874
EheI	GGCGCC	1 cut(s)	1874
ErhI	CCWWGG	2 cut(s)	1191, 2096
Esp3I	CGTCTC	2 cut(s)	2287, 2478
FaeI	CATG	8 cut(s)	310, 676, 853, 998, 1651, 2174, 2254, 2483
FalI	AAGNNNNNCTT	2 cut(s)	1323, 1355
FaqI	GGGAC	4 cut(s)	413, 979, 1426, 2367
FatI	CATG	8 cut(s)	306, 672, 849, 994, 1647, 2170, 2250, 2479
FauI	CCCGC	7 cut(s)	175, 182, 188, 512, 650, 1006, 1687
FokI	GGATG	6 cut(s)	506, 512, 655, 718, 736, 1943
FriOI	GRGCYC	1 cut(s)	276
FspBI	CTAG	5 cut(s)	1491, 1719, 2097, 2135, 2306
GlaI	GCGC	2 cut(s)	227, 1874
GsaI	CCCAGC	1 cut(s)	1447
GsuI	CTGGAG	4 cut(s)	49, 917, 1436, 2402
HaeII	RGCGCY	1 cut(s)	1876
HaeIII	GGCC	5 cut(s)	40, 98, 274, 455, 1335
HapII	CCGG	2 cut(s)	95, 2063
HgaI	GACGC	2 cut(s)	110, 340
HhaI	GCGC	2 cut(s)	228, 1875
Hin1I	GRCGYC	2 cut(s)	102, 1873
Hin1II	CATG	8 cut(s)	310, 676, 853, 998, 1651, 2174, 2254, 2483
Hin6I	GCGC	2 cut(s)	226, 1873
HinP1I	GCGC	2 cut(s)	226, 1873
HincII	GTYRAC	2 cut(s)	1953, 2022
HindII	GTYRAC	2 cut(s)	1953, 2022
HindIII	AAGCTT	1 cut(s)	1466
HpaII	CCGG	2 cut(s)	95, 2063
HphI	GGTGA	6 cut(s)	1037, 1433, 1672, 1979, 2092, 2411
Hpy166II	GTNNAC	4 cut(s)	892, 909, 1953, 2022
Hpy8I	GTNNAC	4 cut(s)	892, 909, 1953, 2022
Hpy99I	CGWCG	1 cut(s)	104
HpyCH4III	ACNGT	8 cut(s)	899, 979, 1093, 1561, 1637, 1678, 1921, 2489
HpyCH4IV	ACGT	2 cut(s)	1949, 2119
HpyF3I	CTNAG	6 cut(s)	540, 753, 1579, 1914, 2123, 2432
HpySE526I	ACGT	2 cut(s)	1949, 2119
Hsp92I	GRCGYC	2 cut(s)	102, 1873
Hsp92II	CATG	8 cut(s)	310, 676, 853, 998, 1651, 2174, 2254, 2483
HspAI	GCGC	2 cut(s)	226, 1873
KasI	GGCGCC	1 cut(s)	1872
KflI	GGGWCCC	1 cut(s)	2354
KpnI	GGTACC	1 cut(s)	556
LmnI	GCTCC	9 cut(s)	182, 936, 1168, 1455, 1477, 1574, 1644, 1666, 2386
Lsp1109I	GCAGC	6 cut(s)	1434, 1681, 1996, 2079, 2082, 2364
LweI	GCATC	6 cut(s)	490, 767, 836, 981, 1000, 2331
MabI	ACCWGGT	1 cut(s)	1021
MaeI	CTAG	5 cut(s)	1491, 1719, 2097, 2135, 2306
MaeII	ACGT	2 cut(s)	1949, 2119
MaeIII	GTNAC	4 cut(s)	791, 1025, 1039, 1650
MbiI	CCGCTC	2 cut(s)	113, 177
MfeI	CAATTG	2 cut(s)	1241, 2442
MflI	RGATCY	6 cut(s)	134, 951, 1289, 1540, 1754, 2254
MhlI	GDGCHC	2 cut(s)	276, 341
Mly113I	GGCGCC	1 cut(s)	1873
MlyI	GAGTC	3 cut(s)	5, 50, 2017
MmeI	TCCRAC	2 cut(s)	33, 1054
Mph1103I	ATGCAT	2 cut(s)	851, 996
MseI	TTAA	6 cut(s)	363, 1404, 1713, 1838, 2262, 2327
MspA1I	CMGCKG	1 cut(s)	1447
MspI	CCGG	2 cut(s)	95, 2063
MspR9I	CCNGG	7 cut(s)	491, 935, 1023, 1428, 1733, 1819, 2064
MunI	CAATTG	2 cut(s)	1241, 2442
Mva1269I	GAATGC	4 cut(s)	152, 1161, 1366, 2443
MvaI	CCWGG	6 cut(s)	491, 935, 1023, 1428, 1733, 1819
MvnI	CGCG	1 cut(s)	226
NarI	GGCGCC	1 cut(s)	1873
NciI	CCSGG	1 cut(s)	2064
NlaIII	CATG	8 cut(s)	310, 676, 853, 998, 1651, 2174, 2254, 2483
NmuCI	GTSAC	1 cut(s)	1025
NsiI	ATGCAT	2 cut(s)	851, 996
NspI	RCATGY	1 cut(s)	1651
PceI	AGGCCT	1 cut(s)	1335
PcsI	WCGNNNNNNNCGW	2 cut(s)	192, 309
PctI	GAATGC	4 cut(s)	152, 1161, 1366, 2443
PfeI	GAWTC	8 cut(s)	485, 524, 1213, 1698, 1909, 2143, 2156, 2302
PflFI	GACNNNGTC	1 cut(s)	2489
PfoI	TCCNGGA	2 cut(s)	933, 1426
Ple19I	CGATCG	1 cut(s)	2247
PleI	GAGTC	3 cut(s)	5, 50, 2017
PluTI	GGCGCC	1 cut(s)	1876
PpsI	GAGTC	3 cut(s)	5, 50, 2017
PpuMI	RGGWCCY	1 cut(s)	2354
PshAI	GACNNNNGTC	1 cut(s)	1727
PshBI	ATTAAT	2 cut(s)	1404, 2262
Psp5II	RGGWCCY	1 cut(s)	2354
Psp6I	CCWGG	6 cut(s)	489, 933, 1021, 1426, 1731, 1817
PspFI	CCCAGC	1 cut(s)	1443
PspGI	CCWGG	6 cut(s)	489, 933, 1021, 1426, 1731, 1817
PspOMI	GGGCCC	1 cut(s)	272
PspPI	GGNCC	8 cut(s)	38, 272, 273, 316, 400, 735, 1076, 2354
PspPPI	RGGWCCY	1 cut(s)	2354
PstI	CTGCAG	1 cut(s)	2014
PsuI	RGATCY	6 cut(s)	134, 951, 1289, 1540, 1754, 2254
PsyI	GACNNNGTC	1 cut(s)	2489
PvuI	CGATCG	1 cut(s)	2247
PvuII	CAGCTG	1 cut(s)	1447
RsaI	GTAC	3 cut(s)	554, 1457, 1923
RsaNI	GTAC	3 cut(s)	553, 1456, 1922
SaqAI	TTAA	6 cut(s)	363, 1404, 1713, 1838, 2262, 2327
Sau96I	GGNCC	8 cut(s)	38, 272, 273, 316, 400, 735, 1076, 2354
SchI	GAGTC	3 cut(s)	5, 50, 2017
ScrFI	CCNGG	7 cut(s)	491, 935, 1023, 1428, 1733, 1819, 2064
SduI	GDGCHC	2 cut(s)	276, 341
SexAI	ACCWGGT	1 cut(s)	1021
SfaNI	GCATC	6 cut(s)	490, 767, 836, 981, 1000, 2331
SfcI	CTRYAG	2 cut(s)	813, 2010
SfoI	GGCGCC	1 cut(s)	1874
SinI	GGWCC	5 cut(s)	316, 400, 735, 1076, 2354
SmlI	CTYRAG	1 cut(s)	560
SmoI	CTYRAG	1 cut(s)	560
SpeI	ACTAGT	1 cut(s)	1490
SseBI	AGGCCT	1 cut(s)	1335
SspDI	GGCGCC	1 cut(s)	1872
SspI	AATATT	2 cut(s)	771, 1408
SspMI	CTAG	5 cut(s)	1491, 1719, 2097, 2135, 2306
StuI	AGGCCT	1 cut(s)	1335
StyD4I	CCNGG	7 cut(s)	489, 933, 1021, 1426, 1731, 1817, 2062
StyI	CCWWGG	2 cut(s)	1191, 2096
TaaI	ACNGT	8 cut(s)	899, 979, 1093, 1561, 1637, 1678, 1921, 2489
TaiI	ACGT	2 cut(s)	1952, 2122
TaqI	TCGA	5 cut(s)	579, 624, 1594, 2106, 2247
TaqII	GACCGA	1 cut(s)	723
TatI	WGTACW	2 cut(s)	1455, 1921
TauI	GCSGC	8 cut(s)	107, 110, 113, 129, 132, 191, 231, 294
TfiI	GAWTC	8 cut(s)	485, 524, 1213, 1698, 1909, 2143, 2156, 2302
Tru1I	TTAA	6 cut(s)	363, 1404, 1713, 1838, 2262, 2327
Tru9I	TTAA	6 cut(s)	363, 1404, 1713, 1838, 2262, 2327
TscAI	CASTG	3 cut(s)	1642, 2242, 2416
TseFI	GTSAC	1 cut(s)	1025
TseI	GCWGC	6 cut(s)	1447, 1669, 2009, 2067, 2070, 2377
Tsp45I	GTSAC	1 cut(s)	1025
TspDTI	ATGAA	6 cut(s)	849, 866, 1239, 1629, 2019, 2468
TspGWI	ACGGA	2 cut(s)	1190, 1382
TspRI	CASTG	3 cut(s)	1642, 2242, 2416
Tth111I	GACNNNGTC	1 cut(s)	2489
VpaK11BI	GGWCC	5 cut(s)	316, 400, 735, 1076, 2354
VspI	ATTAAT	2 cut(s)	1404, 2262
XagI	CCTNNNNNAGG	3 cut(s)	960, 1548, 1847
XapI	RAATTY	9 cut(s)	391, 465, 874, 1186, 1201, 1616, 2056, 2271, 2415
XceI	RCATGY	1 cut(s)	1651
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1352
XmaJI	CCTAGG	1 cut(s)	2096
XspI	CTAG	5 cut(s)	1491, 1719, 2097, 2135, 2306
Zsp2I	ATGCAT	2 cut(s)	851, 996

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.