Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0008419

Physical Location & Seq

Genomic Coordinates Forward (+)

5886161 .. 5888001

Gene Structure 1841 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g04970.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 984 bp

ATGGCTTCCGATTCTTCTCCGGCGGACTTCAACAATGGGCCTTCTTCTCCTGATGACTTCTTATCGAGCCCAATCGGCAACACCTTCTCATCTCCGGTGGACACCACCCACCGCAGTCGGCGTCGATCCATTACCCCCTTGGAATTTGACACCCCGCGGGCGCAGCGGTCCCGGTTCGCCGCATCCGAAACAACACCAACGGGCACACGCCGCACCAACGGCCATAGTGCTGGCGCTGCCCCGACGCCCTCCTCCACTGACGGTGATGTCCCACCATCTTCTGAAGGCGGCGATGGGTACGGTGTGGACGAGGACCGACCCACATTTGTCTGGGGCACGAACATCAGCGTGAACGACGTTAAGGTGGCGATTGTTAGGTTTTTAAAGAATTTTCGGGATGAGTCGCAGTGGAATATGGAGAGTGAGTACCTTACAGAAGGGAAGTACATTGAGGCCATCAAGAGAGTTATTGAGATGGAAGATGATTCGCTTGATGTTGATGCGCGCAATGTGTTTGATTATGATTCCGACTTGTATGCGAAGATGGTCAGGTACCCACTCGAGGTTCTTGCGATTTTCGATATTGTTTTGATGGAAATGCTGCCCGTCATAAACCCGTTGTTCGAAAAGCACATCCAAACTCGGATTTATAATCTCAAAACATCCACCTCGATGAGAAATCTCAACCCATCTGACATTGAGAGGATGGTTTCGTTGAAGGGAATGATTATTCGGTCTAGTTCAATAATACCTGAGATAAGGGAAGCAATATTTAGATGCCTTGTGTGTGGCTACTACTCCGACCCACTTCCTGTAGAGAAAGGGCGAATATCGGAGCCTACAATATGTTTGAAGGAAGAGTGTCAAGCCAGGAATTCCATGACACTGGTTCACAATCGATGCAGATTTACAGATAAACAGATTGTGAGGCTCCAGGAGACTCCTGATGATCGATCCCTGAAGGAGGGACACCACATACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0000785 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

328

Amino Acids

36.9

Weight (kDa)

5.23

Isoelectric Point (pI)

59.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	124 - 218	1e-12	MCM N-terminal domain
MCM_OB	PF17207	225 - 317	2.8e-23	MCM OB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008419)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16440
fragaria_vesca	FvH4_2g09990
malus_domestica	MD00G1085600.v1.1 MD10G1066500.v1.1
prunus_persica	Prupe.8G088400_v2.0.a1
pyrus_communis	pycom05g04970 pycom05g04980 pycom10g05200
rosa_chinensis	RchiOBHm_Chr6g0266881 RchiOBHm_Chr6g0266891
rosa_laevigata	RLG00000014063 RLG00000014064 RLG00000014065
rosa_multiflora	Rmu_co8507775.1_g000001 Rmu_sc0021142.1_g000001 Rmu_sc0036177.1_g000001
rosa_roxburghii	Rroxscaffold_7G00201330
rosa_rugosa	Rorug06G0027900
rosa_samantha	Rh6AG149000 Rh6BG150000 Rh6CG144900 Rh6DG134200
rosa_wichuraiana	Rw6G012890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	651
AasI	GACNNNNNNGTC	1 cut(s)	266
Acc65I	GGTACC	1 cut(s)	552
AccB1I	GGYRCC	1 cut(s)	552
AccII	CGCG	2 cut(s)	157, 505
AciI	CCGC	8 cut(s)	23, 112, 155, 157, 166, 180, 211, 288
AclWI	GGATC	2 cut(s)	120, 948
AcoI	YGGCCR	1 cut(s)	220
AcsI	RAATTY	3 cut(s)	143, 388, 874
AcuI	CTGAAG	2 cut(s)	303, 980
AcyI	GRCGYC	2 cut(s)	121, 245
AfaI	GTAC	4 cut(s)	299, 428, 446, 554
AfiI	CCNNNNNNNGG	2 cut(s)	562, 964
AgsI	TTSAA	4 cut(s)	31, 718, 744, 853
AjnI	CCWGG	2 cut(s)	869, 933
Alw26I	GTCTC	1 cut(s)	932
AlwI	GGATC	2 cut(s)	120, 948
Ama87I	CYCGRG	1 cut(s)	560
AoxI	GGCC	3 cut(s)	38, 220, 453
ApeKI	GCWGC	3 cut(s)	163, 236, 601
ApoI	RAATTY	3 cut(s)	143, 388, 874
Asp718I	GGTACC	1 cut(s)	552
AspLEI	GCGC	4 cut(s)	163, 236, 505, 507
AspS9I	GGNCC	3 cut(s)	38, 168, 313
AsuC2I	CCSGG	1 cut(s)	172
AsuHPI	GGTGA	1 cut(s)	275
AsuII	TTCGAA	1 cut(s)	624
AvaI	CYCGRG	1 cut(s)	560
AvaII	GGWCC	2 cut(s)	168, 313
BaeGI	GKGCMC	2 cut(s)	206, 338
BanI	GGYRCC	1 cut(s)	552
BanII	GRGCYC	1 cut(s)	71
BbvI	GCAGC	3 cut(s)	175, 223, 588
BccI	CCATC	8 cut(s)	283, 287, 464, 469, 538, 586, 697, 700
BceAI	ACGGC	1 cut(s)	235
BcgI	CGANNNNNNTGC	4 cut(s)	518, 551, 552, 585
BciT130I	CCWGG	2 cut(s)	871, 935
BcnI	CCSGG	1 cut(s)	172
BcoDI	GTCTC	1 cut(s)	932
BfaI	CTAG	1 cut(s)	738
BfmI	CTRYAG	1 cut(s)	813
BfoI	RGCGCY	1 cut(s)	237
BglI	GCCNNNNNGGC	1 cut(s)	75
BisI	GCNGC	6 cut(s)	164, 180, 211, 237, 289, 602
BlsI	GCNGC	6 cut(s)	165, 181, 212, 238, 290, 603
Bme1390I	CCNGG	3 cut(s)	172, 871, 935
Bme18I	GGWCC	2 cut(s)	168, 313
BmeT110I	CYCGRG	1 cut(s)	560
BmgT120I	GGNCC	3 cut(s)	38, 168, 313
BmiI	GGNNCC	4 cut(s)	170, 554, 837, 932
BmrFI	CCNGG	3 cut(s)	172, 871, 935
BmsI	GCATC	4 cut(s)	191, 490, 767, 890
BpmI	CTGGAG	1 cut(s)	917
Bpu14I	TTCGAA	1 cut(s)	624
BpuMI	CCSGG	1 cut(s)	172
Bsa29I	ATCGAT	2 cut(s)	898, 952
BsaHI	GRCGYC	2 cut(s)	121, 245
BsaJI	CCNNGG	2 cut(s)	138, 155
BsaWI	WCCGGW	1 cut(s)	94
BsaXI	ACNNNNNCTCC	2 cut(s)	410, 440
Bsc4I	CCNNNNNNNGG	2 cut(s)	562, 964
Bse1I	ACTGG	1 cut(s)	891
Bse3DI	GCAATG	1 cut(s)	514
BseBI	CCWGG	2 cut(s)	871, 935
BseCI	ATCGAT	2 cut(s)	898, 952
BseDI	CCNNGG	2 cut(s)	138, 155
BseGI	GGATG	5 cut(s)	182, 403, 633, 662, 711
BseLI	CCNNNNNNNGG	2 cut(s)	562, 964
BseMI	GCAATG	1 cut(s)	514
BseMII	CTCAG	1 cut(s)	744
BseNI	ACTGG	1 cut(s)	891
BsePI	GCGCGC	1 cut(s)	503
BseRI	GAGGAG	1 cut(s)	241
BseSI	GKGCMC	2 cut(s)	206, 338
BseXI	GCAGC	3 cut(s)	175, 223, 588
Bsh1236I	CGCG	2 cut(s)	157, 505
BshFI	GGCC	3 cut(s)	40, 222, 455
BshNI	GGYRCC	1 cut(s)	552
BshVI	ATCGAT	2 cut(s)	898, 952
BsiHKCI	CYCGRG	1 cut(s)	560
BsiSI	CCGG	3 cut(s)	20, 95, 172
BslFI	GGGAC	2 cut(s)	154, 254
BslI	CCNNNNNNNGG	2 cut(s)	562, 964
BsmAI	GTCTC	1 cut(s)	932
BsmFI	GGGAC	2 cut(s)	154, 254
BsnI	GGCC	3 cut(s)	40, 222, 455
BsoBI	CYCGRG	1 cut(s)	560
Bsp119I	TTCGAA	1 cut(s)	624
Bsp1286I	GDGCHC	3 cut(s)	71, 206, 338
Bsp143I	GATC	3 cut(s)	125, 949, 953
BspACI	CCGC	8 cut(s)	23, 112, 155, 157, 166, 180, 211, 288
BspANI	GGCC	3 cut(s)	40, 222, 455
BspCNI	CTCAG	1 cut(s)	745
BspDI	ATCGAT	2 cut(s)	898, 952
BspFNI	CGCG	2 cut(s)	157, 505
BspLI	GGNNCC	4 cut(s)	170, 554, 837, 932
BspPI	GGATC	2 cut(s)	120, 948
BspT104I	TTCGAA	1 cut(s)	624
BspT107I	GGYRCC	1 cut(s)	552
BsrDI	GCAATG	1 cut(s)	514
BsrI	ACTGG	1 cut(s)	891
BssECI	CCNNGG	2 cut(s)	138, 155
BssHII	GCGCGC	1 cut(s)	503
BssMI	GATC	3 cut(s)	125, 949, 953
BssNI	GRCGYC	2 cut(s)	121, 245
BssT1I	CCWWGG	1 cut(s)	138
Bst2UI	CCWGG	2 cut(s)	871, 935
Bst4CI	ACNGT	3 cut(s)	263, 302, 981
Bst6I	CTCTTC	1 cut(s)	852
BstACI	GRCGYC	2 cut(s)	121, 245
BstBI	TTCGAA	1 cut(s)	624
BstC8I	GCNNGC	3 cut(s)	159, 232, 505
BstDEI	CTNAG	1 cut(s)	753
BstDSI	CCRYGG	1 cut(s)	155
BstENI	CCTNNNNNAGG	1 cut(s)	962
BstF5I	GGATG	5 cut(s)	182, 403, 633, 662, 711
BstFNI	CGCG	2 cut(s)	157, 505
BstH2I	RGCGCY	1 cut(s)	237
BstHHI	GCGC	4 cut(s)	163, 236, 505, 507
BstKTI	GATC	3 cut(s)	128, 952, 956
BstMAI	GTCTC	1 cut(s)	932
BstMBI	GATC	3 cut(s)	125, 949, 953
BstMWI	GCNNNNNNNGC	5 cut(s)	75, 163, 210, 219, 236
BstNI	CCWGG	2 cut(s)	871, 935
BstSCI	CCNGG	3 cut(s)	170, 869, 933
BstSFI	CTRYAG	1 cut(s)	813
BstSLI	GKGCMC	2 cut(s)	206, 338
BstUI	CGCG	2 cut(s)	157, 505
BstV1I	GCAGC	3 cut(s)	175, 223, 588
BstXI	CCANNNNNNTGG	2 cut(s)	230, 886
Bsu15I	ATCGAT	2 cut(s)	898, 952
BsuRI	GGCC	3 cut(s)	40, 222, 455
BsuTUI	ATCGAT	2 cut(s)	898, 952
BtgI	CCRYGG	1 cut(s)	155
BtgZI	GCGATG	1 cut(s)	306
BtsCI	GGATG	5 cut(s)	182, 403, 633, 662, 711
BtsI	GCAGTG	1 cut(s)	413
BtsIMutI	CAGTG	3 cut(s)	255, 413, 884
Cac8I	GCNNGC	3 cut(s)	159, 232, 505
CfoI	GCGC	4 cut(s)	163, 236, 505, 507
Cfr13I	GGNCC	3 cut(s)	38, 168, 313
Cfr42I	CCGCGG	1 cut(s)	158
ClaI	ATCGAT	2 cut(s)	898, 952
CseI	GACGC	2 cut(s)	110, 253
Csp6I	GTAC	4 cut(s)	298, 427, 445, 553
CviAII	CATG	1 cut(s)	880
CviJI	RGCY	9 cut(s)	5, 40, 69, 222, 455, 792, 838, 869, 931
CviKI_1	RGCY	9 cut(s)	5, 40, 69, 222, 455, 792, 838, 869, 931
CviQI	GTAC	4 cut(s)	298, 427, 445, 553
DdeI	CTNAG	1 cut(s)	753
DpnI	GATC	3 cut(s)	127, 951, 955
DpnII	GATC	3 cut(s)	125, 949, 953
DraI	TTTAAA	1 cut(s)	384
DrdI	GACNNNNNNGTC	1 cut(s)	266
DseDI	GACNNNNNNGTC	1 cut(s)	266
EaeI	YGGCCR	1 cut(s)	220
Eam1104I	CTCTTC	1 cut(s)	852
EarI	CTCTTC	1 cut(s)	852
EciI	GGCGGA	1 cut(s)	38
Eco130I	CCWWGG	1 cut(s)	138
Eco24I	GRGCYC	1 cut(s)	71
Eco47I	GGWCC	2 cut(s)	168, 313
Eco57I	CTGAAG	2 cut(s)	303, 980
Eco88I	CYCGRG	1 cut(s)	560
EcoNI	CCTNNNNNAGG	1 cut(s)	962
EcoRI	GAATTC	1 cut(s)	874
EcoRII	CCWGG	2 cut(s)	869, 933
EcoT14I	CCWWGG	1 cut(s)	138
EcoT38I	GRGCYC	1 cut(s)	71
ErhI	CCWWGG	1 cut(s)	138
FaeI	CATG	1 cut(s)	883
FaiI	YATR	9 cut(s)	225, 416, 522, 537, 611, 651, 847, 881, 977
FaqI	GGGAC	2 cut(s)	154, 254
FatI	CATG	1 cut(s)	879
FauI	CCCGC	2 cut(s)	150, 162
Fnu4HI	GCNGC	6 cut(s)	164, 180, 211, 237, 289, 602
FokI	GGATG	5 cut(s)	169, 410, 620, 649, 718
FriOI	GRGCYC	1 cut(s)	71
Fsp4HI	GCNGC	6 cut(s)	164, 180, 211, 237, 289, 602
FspBI	CTAG	1 cut(s)	738
GlaI	GCGC	4 cut(s)	162, 235, 504, 506
GluI	GCNGC	6 cut(s)	164, 180, 211, 237, 289, 602
GsuI	CTGGAG	1 cut(s)	917
HaeII	RGCGCY	1 cut(s)	237
HaeIII	GGCC	3 cut(s)	40, 222, 455
HapII	CCGG	3 cut(s)	20, 95, 172
HgaI	GACGC	2 cut(s)	110, 253
HhaI	GCGC	4 cut(s)	163, 236, 505, 507
Hin1I	GRCGYC	2 cut(s)	121, 245
Hin1II	CATG	1 cut(s)	883
Hin6I	GCGC	4 cut(s)	161, 234, 503, 505
HinP1I	GCGC	4 cut(s)	161, 234, 503, 505
HinfI	GANTC	5 cut(s)	11, 401, 485, 524, 940
HpaII	CCGG	3 cut(s)	20, 95, 172
HphI	GGTGA	1 cut(s)	275
Hpy166II	GTNNAC	4 cut(s)	100, 307, 352, 892
Hpy188I	TCNGA	8 cut(s)	10, 187, 283, 529, 645, 694, 802, 835
Hpy188III	TCNNGA	4 cut(s)	50, 395, 460, 944
Hpy8I	GTNNAC	4 cut(s)	100, 307, 352, 892
Hpy99I	CGWCG	3 cut(s)	126, 247, 359
HpyAV	CCTTC	7 cut(s)	51, 94, 278, 431, 712, 847, 955
HpyCH4III	ACNGT	3 cut(s)	263, 302, 981
HpyCH4IV	ACGT	1 cut(s)	357
HpyCH4V	TGCA	1 cut(s)	903
HpyF10VI	GCNNNNNNNGC	5 cut(s)	75, 163, 210, 219, 236
HpyF3I	CTNAG	1 cut(s)	753
HpySE526I	ACGT	1 cut(s)	357
Hsp92I	GRCGYC	2 cut(s)	121, 245
Hsp92II	CATG	1 cut(s)	883
HspAI	GCGC	4 cut(s)	161, 234, 503, 505
KpnI	GGTACC	1 cut(s)	556
KspI	CCGCGG	1 cut(s)	158
Kzo9I	GATC	3 cut(s)	125, 949, 953
LmnI	GCTCC	2 cut(s)	835, 936
Lsp1109I	GCAGC	3 cut(s)	175, 223, 588
LweI	GCATC	4 cut(s)	191, 490, 767, 890
MaeI	CTAG	1 cut(s)	738
MaeII	ACGT	1 cut(s)	357
MalI	GATC	3 cut(s)	127, 951, 955
MboI	GATC	3 cut(s)	125, 949, 953
MboII	GAAGA	6 cut(s)	6, 36, 270, 491, 553, 869
MhlI	GDGCHC	3 cut(s)	71, 206, 338
MluCI	AATT	3 cut(s)	143, 388, 874
MlyI	GAGTC	2 cut(s)	410, 934
MmeI	TCCRAC	2 cut(s)	552, 825
MnlI	CCTC	9 cut(s)	259, 262, 304, 445, 556, 679, 696, 921, 958
MseI	TTAA	2 cut(s)	360, 383
MslI	CAYNNNNRTG	2 cut(s)	347, 671
MspA1I	CMGCKG	2 cut(s)	157, 166
MspI	CCGG	3 cut(s)	20, 95, 172
MspR9I	CCNGG	3 cut(s)	172, 871, 935
MvaI	CCWGG	2 cut(s)	871, 935
MvnI	CGCG	2 cut(s)	157, 505
MwoI	GCNNNNNNNGC	5 cut(s)	75, 163, 210, 219, 236
NciI	CCSGG	1 cut(s)	172
NdeII	GATC	3 cut(s)	125, 949, 953
NlaIII	CATG	1 cut(s)	883
NlaIV	GGNNCC	4 cut(s)	170, 554, 837, 932
NspV	TTCGAA	1 cut(s)	624
PaeR7I	CTCGAG	1 cut(s)	560
PauI	GCGCGC	1 cut(s)	503
PcsI	WCGNNNNNNNCGW	2 cut(s)	183, 306
PfeI	GAWTC	3 cut(s)	11, 485, 524
PfoI	TCCNGGA	1 cut(s)	933
PkrI	GCNGC	6 cut(s)	165, 181, 212, 238, 290, 603
PleI	GAGTC	2 cut(s)	409, 934
PpsI	GAGTC	2 cut(s)	409, 934
PsiI	TTATAA	1 cut(s)	651
Psp6I	CCWGG	2 cut(s)	869, 933
PspGI	CCWGG	2 cut(s)	869, 933
PspN4I	GGNNCC	4 cut(s)	170, 554, 837, 932
PspPI	GGNCC	3 cut(s)	38, 168, 313
PspXI	VCTCGAGB	1 cut(s)	560
PteI	GCGCGC	1 cut(s)	503
RsaI	GTAC	4 cut(s)	299, 428, 446, 554
RsaNI	GTAC	4 cut(s)	298, 427, 445, 553
RseI	CAYNNNNRTG	2 cut(s)	347, 671
SacII	CCGCGG	1 cut(s)	158
SaqAI	TTAA	2 cut(s)	360, 383
SatI	GCNGC	6 cut(s)	164, 180, 211, 237, 289, 602
Sau3AI	GATC	3 cut(s)	125, 949, 953
Sau96I	GGNCC	3 cut(s)	38, 168, 313
SchI	GAGTC	2 cut(s)	410, 934
ScrFI	CCNGG	3 cut(s)	172, 871, 935
SduI	GDGCHC	3 cut(s)	71, 206, 338
SetI	ASST	9 cut(s)	86, 360, 366, 380, 432, 554, 567, 671, 754
SfaNI	GCATC	4 cut(s)	191, 490, 767, 890
SfcI	CTRYAG	1 cut(s)	813
Sfr274I	CTCGAG	1 cut(s)	560
Sfr303I	CCGCGG	1 cut(s)	158
SfuI	TTCGAA	1 cut(s)	624
SgrBI	CCGCGG	1 cut(s)	158
SinI	GGWCC	2 cut(s)	168, 313
SlaI	CTCGAG	1 cut(s)	560
SmiMI	CAYNNNNRTG	2 cut(s)	347, 671
SmlI	CTYRAG	1 cut(s)	560
SmoI	CTYRAG	1 cut(s)	560
Sse9I	AATT	3 cut(s)	143, 388, 874
SsiI	CCGC	8 cut(s)	23, 112, 155, 157, 166, 180, 211, 288
SspI	AATATT	1 cut(s)	771
SspMI	CTAG	1 cut(s)	738
StyD4I	CCNGG	3 cut(s)	170, 869, 933
StyI	CCWWGG	1 cut(s)	138
TaaI	ACNGT	3 cut(s)	263, 302, 981
TaiI	ACGT	1 cut(s)	360
TaqI	TCGA	8 cut(s)	65, 124, 561, 579, 624, 671, 898, 952
TaqII	GACCGA	2 cut(s)	330, 723
TasI	AATT	3 cut(s)	143, 388, 874
TatI	WGTACW	1 cut(s)	444
TauI	GCSGC	3 cut(s)	182, 213, 291
TfiI	GAWTC	3 cut(s)	11, 485, 524
Tru1I	TTAA	2 cut(s)	360, 383
Tru9I	TTAA	2 cut(s)	360, 383
TscAI	CASTG	3 cut(s)	262, 413, 891
TseI	GCWGC	3 cut(s)	163, 236, 601
TspRI	CASTG	3 cut(s)	262, 413, 891
VpaK11BI	GGWCC	2 cut(s)	168, 313
XagI	CCTNNNNNAGG	1 cut(s)	962
XapI	RAATTY	3 cut(s)	143, 388, 874
XcmI	CCANNNNNNNNNTGG	1 cut(s)	136
XhoI	CTCGAG	1 cut(s)	560
XspI	CTAG	1 cut(s)	738

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.