Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0008419

Physical Location & Seq

Genomic Coordinates Reverse (-)

17161764 .. 17172896

Gene Structure 11133 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1085600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2592 bp

ATGGCTTCCGATTCTTCTCCGGCGGACTTCAACAATGGGCCTTCTTCTCCTGATGACTTCTTATCGAGCCCAATCGGCAACACCTTCTCCTCTCCGGTGGACGCCACCCACCGCAGTCGGCGTCGATCCATTACCCCCTTGGAATTTGACACCCCGCGGGCGCAGCGGTCCCGGTTCGCCGCATCCGAAACAACACCAACGGGCACACACCGCACCAACGGCCATAGTGCTGGCGCTGCCCCGACCCCCTCCTCCACTGACGGTGATGTCCCACCATCTTCTGAAGGCGGCGATGGGTACGGTGTGGACGAGGACCGACCCACATTTGTCTGGGGCACGAACATCAGCGTGAACGATGTTAAGGTGGCGATTGTTAGGTTTTTAAAGAATTTTCGGGATGAGTCGCAGTGGAATACGGAGAGTGAGTACCTTACAGAAGGGAAGTACATTGAGGCCATCAAGAGAGTTGTTGAGACGGAAGATGATTCGCTTGATGTTGATGCACGCAATGTGTTTGATTATGATTCCGACTTGTATGCGAAGATGGTCAGGTACCCACTCGAGGTTCTTGCGATTTTCGATATTGTTTTGATGGAAATGCTGCCCATCATAAACCCGTTGTTCGAAAAGCACATCCAAACTCGGATTTATAATCTCAAAACATCCACCTCGATGAGAAATCTCAACCCATCTGACATTGAGAGGATGGTTTCGTTGAAGGGAATGATTATTCGGTCTAGTTCAATAATACCTGAGATAAGGGAAGCAATATTTAGATGCCTTGTGTGTGGCTACTACTCCGACCCACTTCCTGTAGAGAAAGGGCGAATATCGGAGCCTACAATATGTTTGAAGGAAGAGTGTCAAGCCAGGAATTCCATGACACTGGTTCATAATCGATGCAGATTTACAGATAAACAGATTGTGAGGCTCCAGGAGACTCCTGATGAGATCCCTGAAGGAGGGACACCACATACAGTGAGCTTGTTGATGCATGACAAACTGGTTGATGCTGGAAAGCCAGGAGACAGAATTGAGGTTACTGGGATATACAGGGCAATGACTGTTAGAGTTGGACCGACACAGAGAACTGTAAAATCATTGTTCAAGACTTACATTGATTGTCTGCATATAAAGAAATCTGATAAGTCAAGAATGCTGGCGGAGGATCAAGTGGAAGTTGACACTTCCTTGGTTGGAAACTCAGAAGAGATTCTTTTTGATGAAAAAAAGGTAATCAATGTATACTTTCACTTGCAGTCGCATCAGACTTTGCTTGTATTGTCTGTATCAATCATATCCTTGCTGAAATTTCAGGTGGAACAATTGAAAGGGTTGGCAAAACAGCCAGATATATATGATCGATTAACAAGGTCTTTAGCACCAAACATCTGGGAATTAGATGACGTTAAAAAAGGCCTTCTTTGCCAGCTTTTTGGTGGGAATGCTTTGAAGCTACCATCTGGTGCTCGCTTCCGTGGTGATATTAATATTCTTCTTGTTGGTGATCCTGGAACCAGCAAGTCCCAGCTGCTCCAGTACATACACAAACTAGCTCCTCGCGGAATATACACTAGTGGAAGAGGGAGTTCCGCTGTTGGATTGACTGCTTATGTTGCGAAAGATCCTGAAACAGGGGAAACTGTTTTAGAGAGTGGAGCCTTAGTTTTGAGCGATAGAGGCATTTGTTGCATTGACGAATTTGACAAAATGTCTGATAGTGCGAGGAGCATGCTACATGAGGTGATGGAACAGCAAACTGTTTCAATAGCTAAAGCAGGAATTATTGCTTCCCTTAATGCTAGGACTTCAGTCTTGGCTTGTGCAAATCCAAGTGGTTCACGCTATAACCCTCGCTTATCAGTAATTGACAACATACACCTTCCTCCTACCTTATTATCCAGGTTCGATCTGATCTACTTACTCCTTGACAAGGCTGATGAGCATACAGACAGACGCCTTGCAAAGCATATTGTTGCATTACACTTTGAGAATCCTGAGAGCGTACAGCAGGATGTCTTAGACATTGCTATGTTGACCGCATATGTGGGCTATGCCCGAAAGCATATTCACCCACAATTATCCGATGAGGCTGCTGAAGAGTTGACTAGAGGTTATGTTGAGTTGAGGAGGAGAGGAAATTTTCCAGGCAGTAGCAAAAAGGTGATAACAGCAACACCCAGGCAGATTGAGAGTTTGATACGACTTAGTGAAGCTCTAGCTCGCATCCGTTTCTCAGAATCGGTTGAGAAGCATGATGTTATTGAGGCATTTCGGCTTCTGGAAGTTGCAATGCAGCAATCGGCAACTGACCACTCCACAGGAACGATTGACATGGATCTTATCACCACTGGAATTTCAGCAAGTGAAAGAATGAGACGAGAAAGTCTGGTATCATCAGCTCGCAACATAATTATGGAGAAGATACAACTTGGGGGACCCTCAATGCGCTTGTTAGAGCTGCTGGAGGAGCTGAAGCAGCAGAGCTCTGGCGGTGAACTCCACCTCAACGATCTGAGGACTGCAATTACGACACTAGCAAGTGAGGGATTTGTGGCTTTTGTTCACGGCGACAGTGTCAAAAGAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0000785 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM_N MCM_OB

Protein Analysis

864

Amino Acids

95.98

Weight (kDa)

5.84

Isoelectric Point (pI)

47.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	124 - 218	6.2e-12	MCM N-terminal domain
MCM_OB	PF17207	225 - 354	6.5e-39	MCM OB domain
MCM	PF00493	440 - 662	4.5e-100	MCM P-loop domain
AAA_5	PF07728	497 - 613	8.3e-07	AAA domain (dynein-related subfamily)
MCM_lid	PF17855	678 - 764	4.1e-26	MCM AAA-lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008419)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16440
fragaria_vesca	FvH4_2g09990
malus_domestica	MD00G1085600.v1.1 MD10G1066500.v1.1
prunus_persica	Prupe.8G088400_v2.0.a1
pyrus_communis	pycom05g04970 pycom05g04980 pycom10g05200
rosa_chinensis	RchiOBHm_Chr6g0266881 RchiOBHm_Chr6g0266891
rosa_laevigata	RLG00000014063 RLG00000014064 RLG00000014065
rosa_multiflora	Rmu_co8507775.1_g000001 Rmu_sc0021142.1_g000001 Rmu_sc0036177.1_g000001
rosa_roxburghii	Rroxscaffold_7G00201330
rosa_rugosa	Rorug06G0027900
rosa_samantha	Rh6AG149000 Rh6BG150000 Rh6CG144900 Rh6DG134200
rosa_wichuraiana	Rw6G012890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	651
AasI	GACNNNNNNGTC	2 cut(s)	266, 2385
Acc65I	GGTACC	1 cut(s)	552
AccB1I	GGYRCC	1 cut(s)	552
AccI	GTMKAC	1 cut(s)	1245
AccII	CGCG	2 cut(s)	157, 1563
AclWI	GGATC	6 cut(s)	120, 946, 1176, 1502, 1619, 2346
AcoI	YGGCCR	1 cut(s)	220
AcsI	RAATTY	7 cut(s)	143, 388, 874, 1310, 1700, 2140, 2355
AcuI	CTGAAG	5 cut(s)	303, 978, 1794, 2118, 2495
AcyI	GRCGYC	3 cut(s)	102, 121, 1957
AfaI	GTAC	6 cut(s)	299, 428, 446, 554, 1541, 2007
AfiI	CCNNNNNNNGG	4 cut(s)	562, 962, 1634, 1933
AgsI	TTSAA	8 cut(s)	31, 718, 744, 853, 1108, 1330, 1453, 1767
AhdI	GACNNNNNGTC	1 cut(s)	1711
AhlI	ACTAGT	1 cut(s)	1574
AjnI	CCWGG	7 cut(s)	869, 933, 1021, 1510, 1901, 2146, 2180
Alw21I	GWGCWC	2 cut(s)	1471, 2489
Alw26I	GTCTC	4 cut(s)	467, 932, 1020, 2371
AlwI	GGATC	6 cut(s)	120, 946, 1176, 1502, 1619, 2346
Ama87I	CYCGRG	1 cut(s)	560
AoxI	GGCC	4 cut(s)	38, 220, 453, 1417
ApeKI	GCWGC	8 cut(s)	163, 236, 601, 1531, 2093, 2296, 2461, 2479
ApoI	RAATTY	7 cut(s)	143, 388, 874, 1310, 1700, 2140, 2355
AseI	ATTAAT	1 cut(s)	1488
Asp700I	GAANNNNTTC	1 cut(s)	1212
Asp718I	GGTACC	1 cut(s)	552
AspLEI	GCGC	3 cut(s)	163, 236, 2451
AspS9I	GGNCC	5 cut(s)	38, 168, 313, 1076, 2438
AsuC2I	CCSGG	1 cut(s)	172
AsuHPI	GGTGA	8 cut(s)	275, 1493, 1517, 1756, 2063, 2176, 2338, 2507
AsuII	TTCGAA	1 cut(s)	624
AvaI	CYCGRG	1 cut(s)	560
AvaII	GGWCC	4 cut(s)	168, 313, 1076, 2438
BaeGI	GKGCMC	2 cut(s)	206, 338
BanI	GGYRCC	1 cut(s)	552
BanII	GRGCYC	2 cut(s)	71, 2489
Bbv12I	GWGCWC	2 cut(s)	1471, 2489
BbvI	GCAGC	8 cut(s)	175, 223, 588, 1518, 2080, 2308, 2448, 2491
BceAI	ACGGC	2 cut(s)	235, 2584
BcgI	CGANNNNNNTGC	8 cut(s)	518, 551, 552, 585, 1714, 1748, 2075, 2109
BciT130I	CCWGG	7 cut(s)	871, 935, 1023, 1512, 1903, 2148, 2182
BcnI	CCSGG	1 cut(s)	172
BcoDI	GTCTC	4 cut(s)	467, 932, 1020, 2371
BcuI	ACTAGT	1 cut(s)	1574
BfaI	CTAG	7 cut(s)	738, 1553, 1575, 1803, 2109, 2219, 2537
BfmI	CTRYAG	1 cut(s)	813
BfoI	RGCGCY	1 cut(s)	237
BglI	GCCNNNNNGGC	1 cut(s)	75
Bme1390I	CCNGG	8 cut(s)	172, 871, 935, 1023, 1512, 1903, 2148, 2182
Bme18I	GGWCC	4 cut(s)	168, 313, 1076, 2438
BmeRI	GACNNNNNGTC	1 cut(s)	1711
BmeT110I	CYCGRG	1 cut(s)	560
BmgT120I	GGNCC	5 cut(s)	38, 168, 313, 1076, 2438
BmiI	GGNNCC	8 cut(s)	170, 554, 837, 932, 1516, 1660, 2439, 2440
BmrFI	CCNGG	8 cut(s)	172, 871, 935, 1023, 1512, 1903, 2148, 2182
BmrI	ACTGGG	1 cut(s)	1053
BmsI	GCATC	8 cut(s)	191, 490, 767, 890, 981, 1000, 1273, 2235
BmuI	ACTGGG	1 cut(s)	1053
BoxI	GACNNNNGTC	1 cut(s)	1811
BpmI	CTGGAG	3 cut(s)	917, 1520, 2486
Bpu14I	TTCGAA	1 cut(s)	624
BpuMI	CCSGG	1 cut(s)	172
Bsa29I	ATCGAT	2 cut(s)	898, 1363
BsaHI	GRCGYC	3 cut(s)	102, 121, 1957
BsaJI	CCNNGG	5 cut(s)	138, 155, 1191, 1477, 2180
BsaWI	WCCGGW	1 cut(s)	94
BsaXI	ACNNNNNCTCC	6 cut(s)	71, 101, 410, 440, 1650, 1680
Bsc4I	CCNNNNNNNGG	4 cut(s)	562, 962, 1634, 1933
Bse1I	ACTGG	5 cut(s)	891, 1008, 1048, 1537, 2356
Bse3DI	GCAATG	4 cut(s)	514, 1065, 2025, 2298
BseBI	CCWGG	7 cut(s)	871, 935, 1023, 1512, 1903, 2148, 2182
BseCI	ATCGAT	2 cut(s)	898, 1363
BseDI	CCNNGG	5 cut(s)	138, 155, 1191, 1477, 2180
BseGI	GGATG	7 cut(s)	182, 403, 633, 662, 711, 2020, 2226
BseLI	CCNNNNNNNGG	4 cut(s)	562, 962, 1634, 1933
BseMI	GCAATG	4 cut(s)	514, 1065, 2025, 2298
BseMII	CTCAG	5 cut(s)	744, 1218, 1989, 2250, 2507
BseNI	ACTGG	5 cut(s)	891, 1008, 1048, 1537, 2356
BseRI	GAGGAG	7 cut(s)	79, 241, 1548, 1741, 2143, 2146, 2483
BseSI	GKGCMC	2 cut(s)	206, 338
BseXI	GCAGC	8 cut(s)	175, 223, 588, 1518, 2080, 2308, 2448, 2491
BseYI	CCCAGC	1 cut(s)	1527
Bsh1236I	CGCG	2 cut(s)	157, 1563
BshFI	GGCC	4 cut(s)	40, 222, 455, 1419
BshNI	GGYRCC	1 cut(s)	552
BshVI	ATCGAT	2 cut(s)	898, 1363
BsiHKAI	GWGCWC	2 cut(s)	1471, 2489
BsiHKCI	CYCGRG	1 cut(s)	560
BsiSI	CCGG	3 cut(s)	20, 95, 172
BslFI	GGGAC	5 cut(s)	154, 254, 979, 1510, 2451
BslI	CCNNNNNNNGG	4 cut(s)	562, 962, 1634, 1933
BsmAI	GTCTC	4 cut(s)	467, 932, 1020, 2371
BsmBI	CGTCTC	2 cut(s)	467, 2371
BsmFI	GGGAC	5 cut(s)	154, 254, 979, 1510, 2451
BsmI	GAATGC	2 cut(s)	1161, 1450
BsnI	GGCC	4 cut(s)	40, 222, 455, 1419
BsoBI	CYCGRG	1 cut(s)	560
Bsp119I	TTCGAA	1 cut(s)	624
Bsp1286I	GDGCHC	5 cut(s)	71, 206, 338, 1471, 2489
BspANI	GGCC	4 cut(s)	40, 222, 455, 1419
BspCNI	CTCAG	5 cut(s)	745, 1217, 1990, 2249, 2508
BspDI	ATCGAT	2 cut(s)	898, 1363
BspFNI	CGCG	2 cut(s)	157, 1563
BspLI	GGNNCC	8 cut(s)	170, 554, 837, 932, 1516, 1660, 2439, 2440
BspPI	GGATC	6 cut(s)	120, 946, 1176, 1502, 1619, 2346
BspT104I	TTCGAA	1 cut(s)	624
BspT107I	GGYRCC	1 cut(s)	552
BsrDI	GCAATG	4 cut(s)	514, 1065, 2025, 2298
BsrI	ACTGG	5 cut(s)	891, 1008, 1048, 1537, 2356
BssECI	CCNNGG	5 cut(s)	138, 155, 1191, 1477, 2180
BssNAI	GTATAC	1 cut(s)	1246
BssNI	GRCGYC	3 cut(s)	102, 121, 1957
BssT1I	CCWWGG	2 cut(s)	138, 1191
Bst1107I	GTATAC	1 cut(s)	1246
Bst2UI	CCWGG	7 cut(s)	871, 935, 1023, 1512, 1903, 2148, 2182
Bst4CI	ACNGT	8 cut(s)	263, 302, 979, 1066, 1093, 1645, 1762, 2576
Bst6I	CTCTTC	4 cut(s)	852, 1203, 1576, 2094
BstACI	GRCGYC	3 cut(s)	102, 121, 1957
BstAPI	GCANNNNNTGC	1 cut(s)	1689
BstBI	TTCGAA	1 cut(s)	624
BstC8I	GCNNGC	9 cut(s)	159, 232, 505, 1161, 1430, 1471, 1733, 2224, 2404
BstDEI	CTNAG	8 cut(s)	753, 1204, 1663, 1998, 2020, 2207, 2236, 2516
BstDSI	CCRYGG	2 cut(s)	155, 1477
BstENI	CCTNNNNNAGG	3 cut(s)	960, 1632, 1931
BstF5I	GGATG	7 cut(s)	182, 403, 633, 662, 711, 2020, 2226
BstFNI	CGCG	2 cut(s)	157, 1563
BstH2I	RGCGCY	1 cut(s)	237
BstHHI	GCGC	3 cut(s)	163, 236, 2451
BstMAI	GTCTC	4 cut(s)	467, 932, 1020, 2371
BstNI	CCWGG	7 cut(s)	871, 935, 1023, 1512, 1903, 2148, 2182
BstNSI	RCATGY	1 cut(s)	1735
BstPAI	GACNNNNGTC	1 cut(s)	1811
BstSCI	CCNGG	8 cut(s)	170, 869, 933, 1021, 1510, 1901, 2146, 2180
BstSFI	CTRYAG	1 cut(s)	813
BstSLI	GKGCMC	2 cut(s)	206, 338
BstUI	CGCG	2 cut(s)	157, 1563
BstV1I	GCAGC	8 cut(s)	175, 223, 588, 1518, 2080, 2308, 2448, 2491
BstX2I	RGATCY	3 cut(s)	951, 1624, 2338
BstXI	CCANNNNNNTGG	4 cut(s)	230, 886, 1392, 1436
BstYI	RGATCY	3 cut(s)	951, 1624, 2338
BstZ17I	GTATAC	1 cut(s)	1246
Bsu15I	ATCGAT	2 cut(s)	898, 1363
BsuRI	GGCC	4 cut(s)	40, 222, 455, 1419
BsuTUI	ATCGAT	2 cut(s)	898, 1363
BtgI	CCRYGG	2 cut(s)	155, 1477
BtgZI	GCGATG	1 cut(s)	306
BtsCI	GGATG	7 cut(s)	182, 403, 633, 662, 711, 2020, 2226
BtsI	GCAGTG	1 cut(s)	413
BtsIMutI	CAGTG	6 cut(s)	255, 413, 884, 984, 2349, 2581
Cac8I	GCNNGC	9 cut(s)	159, 232, 505, 1161, 1430, 1471, 1733, 2224, 2404
CfoI	GCGC	3 cut(s)	163, 236, 2451
Cfr13I	GGNCC	5 cut(s)	38, 168, 313, 1076, 2438
Cfr42I	CCGCGG	1 cut(s)	158
ClaI	ATCGAT	2 cut(s)	898, 1363
CseI	GACGC	3 cut(s)	110, 110, 1965
Csp6I	GTAC	6 cut(s)	298, 427, 445, 553, 1540, 2006
CviAII	CATG	6 cut(s)	880, 995, 1732, 1739, 2255, 2335
CviQI	GTAC	6 cut(s)	298, 427, 445, 553, 1540, 2006
DdeI	CTNAG	8 cut(s)	753, 1204, 1663, 1998, 2020, 2207, 2236, 2516
DraI	TTTAAA	1 cut(s)	384
DrdI	GACNNNNNNGTC	2 cut(s)	266, 2385
DriI	GACNNNNNGTC	1 cut(s)	1711
DseDI	GACNNNNNNGTC	2 cut(s)	266, 2385
EaeI	YGGCCR	1 cut(s)	220
Eam1104I	CTCTTC	4 cut(s)	852, 1203, 1576, 2094
Eam1105I	GACNNNNNGTC	1 cut(s)	1711
EarI	CTCTTC	4 cut(s)	852, 1203, 1576, 2094
EciI	GGCGGA	2 cut(s)	38, 1178
Ecl136II	GAGCTC	1 cut(s)	2487
Eco130I	CCWWGG	2 cut(s)	138, 1191
Eco147I	AGGCCT	1 cut(s)	1419
Eco24I	GRGCYC	2 cut(s)	71, 2489
Eco47I	GGWCC	4 cut(s)	168, 313, 1076, 2438
Eco53kI	GAGCTC	1 cut(s)	2487
Eco57I	CTGAAG	5 cut(s)	303, 978, 1794, 2118, 2495
Eco88I	CYCGRG	1 cut(s)	560
EcoICRI	GAGCTC	1 cut(s)	2487
EcoNI	CCTNNNNNAGG	3 cut(s)	960, 1632, 1931
EcoO109I	RGGNCCY	1 cut(s)	2438
EcoRI	GAATTC	1 cut(s)	874
EcoRII	CCWGG	7 cut(s)	869, 933, 1021, 1510, 1901, 2146, 2180
EcoT14I	CCWWGG	2 cut(s)	138, 1191
EcoT22I	ATGCAT	1 cut(s)	996
EcoT38I	GRGCYC	2 cut(s)	71, 2489
ErhI	CCWWGG	2 cut(s)	138, 1191
Esp3I	CGTCTC	2 cut(s)	467, 2371
FaeI	CATG	6 cut(s)	883, 998, 1735, 1742, 2258, 2338
FalI	AAGNNNNNCTT	4 cut(s)	1200, 1232, 1407, 1439
FaqI	GGGAC	5 cut(s)	154, 254, 979, 1510, 2451
FatI	CATG	6 cut(s)	879, 994, 1731, 1738, 2254, 2334
FauI	CCCGC	2 cut(s)	150, 162
FauNDI	CATATG	1 cut(s)	2044
FblI	GTMKAC	1 cut(s)	1245
FokI	GGATG	7 cut(s)	169, 410, 620, 649, 718, 2027, 2213
FriOI	GRGCYC	2 cut(s)	71, 2489
FspBI	CTAG	7 cut(s)	738, 1553, 1575, 1803, 2109, 2219, 2537
GlaI	GCGC	3 cut(s)	162, 235, 2450
GsaI	CCCAGC	1 cut(s)	1531
GsuI	CTGGAG	3 cut(s)	917, 1520, 2486
HaeII	RGCGCY	1 cut(s)	237
HaeIII	GGCC	4 cut(s)	40, 222, 455, 1419
HapII	CCGG	3 cut(s)	20, 95, 172
HgaI	GACGC	3 cut(s)	110, 110, 1965
HhaI	GCGC	3 cut(s)	163, 236, 2451
Hin1I	GRCGYC	3 cut(s)	102, 121, 1957
Hin1II	CATG	6 cut(s)	883, 998, 1735, 1742, 2258, 2338
Hin6I	GCGC	3 cut(s)	161, 234, 2449
HinP1I	GCGC	3 cut(s)	161, 234, 2449
HincII	GTYRAC	3 cut(s)	1183, 2037, 2106
HindII	GTYRAC	3 cut(s)	1183, 2037, 2106
HinfI	GANTC	8 cut(s)	11, 401, 485, 524, 940, 1213, 1993, 2240
HpaII	CCGG	3 cut(s)	20, 95, 172
HphI	GGTGA	8 cut(s)	275, 1493, 1517, 1756, 2063, 2176, 2338, 2507
Hpy188III	TCNNGA	9 cut(s)	50, 395, 460, 944, 1108, 1152, 1628, 1997, 2282
Hpy99I	CGWCG	1 cut(s)	126
HpyAV	CCTTC	9 cut(s)	51, 94, 278, 431, 712, 847, 953, 1430, 1892
HpyCH4III	ACNGT	8 cut(s)	263, 302, 979, 1066, 1093, 1645, 1762, 2576
HpyCH4IV	ACGT	1 cut(s)	1407
HpyF3I	CTNAG	8 cut(s)	753, 1204, 1663, 1998, 2020, 2207, 2236, 2516
HpySE526I	ACGT	1 cut(s)	1407
Hsp92I	GRCGYC	3 cut(s)	102, 121, 1957
Hsp92II	CATG	6 cut(s)	883, 998, 1735, 1742, 2258, 2338
HspAI	GCGC	3 cut(s)	161, 234, 2449
KflI	GGGWCCC	1 cut(s)	2438
KpnI	GGTACC	1 cut(s)	556
KspI	CCGCGG	1 cut(s)	158
LmnI	GCTCC	7 cut(s)	835, 936, 1539, 1561, 1658, 1728, 2470
Lsp1109I	GCAGC	8 cut(s)	175, 223, 588, 1518, 2080, 2308, 2448, 2491
LweI	GCATC	8 cut(s)	191, 490, 767, 890, 981, 1000, 1273, 2235
MaeI	CTAG	7 cut(s)	738, 1553, 1575, 1803, 2109, 2219, 2537
MaeII	ACGT	1 cut(s)	1407
MaeIII	GTNAC	1 cut(s)	1039
MfeI	CAATTG	1 cut(s)	1325
MflI	RGATCY	3 cut(s)	951, 1624, 2338
MhlI	GDGCHC	5 cut(s)	71, 206, 338, 1471, 2489
MlyI	GAGTC	2 cut(s)	410, 934
MmeI	TCCRAC	5 cut(s)	552, 825, 1054, 1177, 1579
Mph1103I	ATGCAT	1 cut(s)	996
MroXI	GAANNNNTTC	1 cut(s)	1212
MseI	TTAA	6 cut(s)	360, 383, 1367, 1410, 1488, 1797
MslI	CAYNNNNRTG	4 cut(s)	347, 671, 2030, 2414
MspA1I	CMGCKG	4 cut(s)	157, 166, 1531, 1595
MspI	CCGG	3 cut(s)	20, 95, 172
MspR9I	CCNGG	8 cut(s)	172, 871, 935, 1023, 1512, 1903, 2148, 2182
MunI	CAATTG	1 cut(s)	1325
Mva1269I	GAATGC	2 cut(s)	1161, 1450
MvaI	CCWGG	7 cut(s)	871, 935, 1023, 1512, 1903, 2148, 2182
MvnI	CGCG	2 cut(s)	157, 1563
NciI	CCSGG	1 cut(s)	172
NdeI	CATATG	1 cut(s)	2044
NlaIII	CATG	6 cut(s)	883, 998, 1735, 1742, 2258, 2338
NlaIV	GGNNCC	8 cut(s)	170, 554, 837, 932, 1516, 1660, 2439, 2440
NsiI	ATGCAT	1 cut(s)	996
NspI	RCATGY	1 cut(s)	1735
NspV	TTCGAA	1 cut(s)	624
PaeI	GCATGC	1 cut(s)	1735
PaeR7I	CTCGAG	1 cut(s)	560
PceI	AGGCCT	1 cut(s)	1419
PcsI	WCGNNNNNNNCGW	2 cut(s)	183, 306
PctI	GAATGC	2 cut(s)	1161, 1450
PdmI	GAANNNNTTC	1 cut(s)	1212
PfeI	GAWTC	6 cut(s)	11, 485, 524, 1213, 1993, 2240
PflFI	GACNNNGTC	1 cut(s)	2576
PfoI	TCCNGGA	2 cut(s)	933, 1510
PleI	GAGTC	2 cut(s)	409, 934
PpsI	GAGTC	2 cut(s)	409, 934
PpuMI	RGGWCCY	1 cut(s)	2438
PshAI	GACNNNNGTC	1 cut(s)	1811
PshBI	ATTAAT	1 cut(s)	1488
PsiI	TTATAA	1 cut(s)	651
Psp124BI	GAGCTC	1 cut(s)	2489
Psp5II	RGGWCCY	1 cut(s)	2438
Psp6I	CCWGG	7 cut(s)	869, 933, 1021, 1510, 1901, 2146, 2180
PspFI	CCCAGC	1 cut(s)	1527
PspGI	CCWGG	7 cut(s)	869, 933, 1021, 1510, 1901, 2146, 2180
PspN4I	GGNNCC	8 cut(s)	170, 554, 837, 932, 1516, 1660, 2439, 2440
PspPI	GGNCC	5 cut(s)	38, 168, 313, 1076, 2438
PspPPI	RGGWCCY	1 cut(s)	2438
PspXI	VCTCGAGB	1 cut(s)	560
PsuI	RGATCY	3 cut(s)	951, 1624, 2338
PsyI	GACNNNGTC	1 cut(s)	2576
PvuII	CAGCTG	1 cut(s)	1531
RsaI	GTAC	6 cut(s)	299, 428, 446, 554, 1541, 2007
RsaNI	GTAC	6 cut(s)	298, 427, 445, 553, 1540, 2006
RseI	CAYNNNNRTG	4 cut(s)	347, 671, 2030, 2414
SacI	GAGCTC	1 cut(s)	2489
SacII	CCGCGG	1 cut(s)	158
SaqAI	TTAA	6 cut(s)	360, 383, 1367, 1410, 1488, 1797
Sau96I	GGNCC	5 cut(s)	38, 168, 313, 1076, 2438
SchI	GAGTC	2 cut(s)	410, 934
ScrFI	CCNGG	8 cut(s)	172, 871, 935, 1023, 1512, 1903, 2148, 2182
SduI	GDGCHC	5 cut(s)	71, 206, 338, 1471, 2489
SfaNI	GCATC	8 cut(s)	191, 490, 767, 890, 981, 1000, 1273, 2235
SfcI	CTRYAG	1 cut(s)	813
Sfr274I	CTCGAG	1 cut(s)	560
Sfr303I	CCGCGG	1 cut(s)	158
SfuI	TTCGAA	1 cut(s)	624
SgrBI	CCGCGG	1 cut(s)	158
SinI	GGWCC	4 cut(s)	168, 313, 1076, 2438
SlaI	CTCGAG	1 cut(s)	560
SmiMI	CAYNNNNRTG	4 cut(s)	347, 671, 2030, 2414
SmlI	CTYRAG	1 cut(s)	560
SmoI	CTYRAG	1 cut(s)	560
SpeI	ACTAGT	1 cut(s)	1574
SphI	GCATGC	1 cut(s)	1735
SseBI	AGGCCT	1 cut(s)	1419
SspI	AATATT	2 cut(s)	771, 1492
SspMI	CTAG	7 cut(s)	738, 1553, 1575, 1803, 2109, 2219, 2537
SstI	GAGCTC	1 cut(s)	2489
StuI	AGGCCT	1 cut(s)	1419
StyD4I	CCNGG	8 cut(s)	170, 869, 933, 1021, 1510, 1901, 2146, 2180
StyI	CCWWGG	2 cut(s)	138, 1191
TaaI	ACNGT	8 cut(s)	263, 302, 979, 1066, 1093, 1645, 1762, 2576
TaiI	ACGT	1 cut(s)	1410
TaqI	TCGA	9 cut(s)	65, 124, 561, 579, 624, 671, 898, 1363, 1908
TaqII	GACCGA	3 cut(s)	330, 723, 1093
TatI	WGTACW	2 cut(s)	444, 1539
TauI	GCSGC	2 cut(s)	182, 291
TfiI	GAWTC	6 cut(s)	11, 485, 524, 1213, 1993, 2240
Tru1I	TTAA	6 cut(s)	360, 383, 1367, 1410, 1488, 1797
Tru9I	TTAA	6 cut(s)	360, 383, 1367, 1410, 1488, 1797
TscAI	CASTG	6 cut(s)	262, 413, 891, 984, 2356, 2581
TseI	GCWGC	8 cut(s)	163, 236, 601, 1531, 2093, 2296, 2461, 2479
TspDTI	ATGAA	2 cut(s)	881, 1239
TspGWI	ACGGA	4 cut(s)	431, 491, 1466, 2219
TspRI	CASTG	6 cut(s)	262, 413, 891, 984, 2356, 2581
Tth111I	GACNNNGTC	1 cut(s)	2576
VpaK11BI	GGWCC	4 cut(s)	168, 313, 1076, 2438
VspI	ATTAAT	1 cut(s)	1488
XagI	CCTNNNNNAGG	3 cut(s)	960, 1632, 1931
XapI	RAATTY	7 cut(s)	143, 388, 874, 1310, 1700, 2140, 2355
XceI	RCATGY	1 cut(s)	1735
XcmI	CCANNNNNNNNNTGG	2 cut(s)	136, 1436
XhoI	CTCGAG	1 cut(s)	560
XmiI	GTMKAC	1 cut(s)	1245
XmnI	GAANNNNTTC	1 cut(s)	1212
XspI	CTAG	7 cut(s)	738, 1553, 1575, 1803, 2109, 2219, 2537
Zsp2I	ATGCAT	1 cut(s)	996

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.