AT2G18350

ZF-HD protein dimerisation region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008478
Physical Location & Seq
Reverse (-)
7971017 .. 7972396
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G18350.1

Sequence Viewer

Length: 789 bp
ATGGAAGTTAGAGAGAAGAAAGATGAGAAAATGGAGATGACAAGAAGAAAATCATCAGCTCTAGATCATCATCGTCTTCCTCCGTACACTTACTCCCAAACCGCCAACAAAGAGAAACCCACCACCAAAAGAAACGGGTCGGACCCGGATCCGGATCCGGATCTTGATACTAACCCAATTTCTATATCTCATGCTCCAAGATCATACGCTAGGCCTCAAACTACTTCTCCGGGGAAGGCAAGGTATAGAGAATGCCAAAAGAATCACGCGGCGAGCTCCGGTGGACACGTTGTCGACGGTTGCGGCGAGTTTATGTCTTCCGGGGAAGAAGGCACGGTGGAGTCTCTTCTTTGTGCAGCTTGTGATTGCCACCGGAGCTTCCATAGAAAAGAGATCGATGGTTTGTTTGTTGTCAATTTCAATAGCTTTGGCCATTCACAGAGACCACTCGGAAGCCGCCACGTGTCTCCGATAATGATGAGTTTTGGTGGTGGTGGTGGTTGTGCGGCGGAGTCATCGACGGAGGATCTAAACAAGTTTCATCAATCTTTTAGTGGTTATGGAGTGGATCAGTTTCATCATTATCAACCCAAGAAACGGTTTAGGACAAAGTTTAATGAAGAACAGAAGGAGAAGATGATGGAGTTTGCTGAGAAGATTGGTTGGAGAATGACAAAACTAGAAGATGATGAAGTGAATCGGTTTTGTCGTGAGATTAAAGTTAAAAGACAAGTGTTTAAAGTTTGGATGCACAACAATAAACAAGCAGCAAAGAAGAAGGATTTGTAA

Protein Analysis

262

Amino Acids

29.95

Weight (kDa)

9.07

Isoelectric Point (pI)

57.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZF-HD_dimer PF04770 80 - 132 3.2e-29 ZF-HD protein dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 294
AccII CGCG 1 cut(s) 269
AccIII TCCGGA 2 cut(s) 151, 157
AciI CCGC 6 cut(s) 102, 269, 303, 457, 506, 509
AclWI GGATC 7 cut(s) 143, 149, 156, 162, 168, 534, 576
AcoI YGGCCR 1 cut(s) 430
AcvI CACGTG 1 cut(s) 463
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 2 cut(s) 151, 597
AflIII ACRYGT 2 cut(s) 286, 462
AgsI TTSAA 1 cut(s) 421
AhdI GACNNNNNGTC 1 cut(s) 290
AluBI AGCT 5 cut(s) 59, 276, 359, 378, 426
AluI AGCT 5 cut(s) 59, 276, 359, 378, 426
Alw21I GWGCWC 1 cut(s) 278
Alw26I GTCTC 3 cut(s) 348, 436, 471
AlwI GGATC 7 cut(s) 143, 149, 156, 162, 168, 534, 576
Aor13HI TCCGGA 2 cut(s) 151, 157
AoxI GGCC 2 cut(s) 212, 430
ApeKI GCWGC 2 cut(s) 356, 767
AspS9I GGNCC 1 cut(s) 142
AsuC2I CCSGG 3 cut(s) 146, 231, 322
AvaII GGWCC 1 cut(s) 142
BalI TGGCCA 1 cut(s) 432
BamHI GGATCC 2 cut(s) 148, 154
BanII GRGCYC 1 cut(s) 278
BbrPI CACGTG 1 cut(s) 463
BbsI GAAGAC 2 cut(s) 68, 309
Bbv12I GWGCWC 1 cut(s) 278
BbvI GCAGC 2 cut(s) 368, 779
BccI CCATC 2 cut(s) 392, 634
BcnI CCSGG 3 cut(s) 146, 231, 322
BcoDI GTCTC 3 cut(s) 348, 436, 471
BfaI CTAG 3 cut(s) 62, 210, 680
BisI GCNGC 6 cut(s) 270, 304, 357, 457, 507, 768
BlsI GCNGC 6 cut(s) 271, 305, 358, 458, 508, 769
Bme1390I CCNGG 3 cut(s) 146, 231, 322
Bme18I GGWCC 1 cut(s) 142
BmeRI GACNNNNNGTC 1 cut(s) 290
BmgT120I GGNCC 1 cut(s) 142
BmiI GGNNCC 3 cut(s) 144, 150, 156
BmrFI CCNGG 3 cut(s) 146, 231, 322
BmsI GCATC 1 cut(s) 738
BpiI GAAGAC 2 cut(s) 68, 309
BpuMI CCSGG 3 cut(s) 146, 231, 322
Bsa29I ATCGAT 1 cut(s) 396
BsaAI YACGTR 1 cut(s) 463
BsaBI GATNNNNATC 3 cut(s) 69, 153, 159
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 2 cut(s) 230, 321
BsaWI WCCGGW 4 cut(s) 151, 157, 278, 372
BsaXI ACNNNNNCTCC 4 cut(s) 77, 107, 211, 241
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 597
Bse8I GATNNNNATC 3 cut(s) 69, 153, 159
BseAI TCCGGA 2 cut(s) 151, 157
BseCI ATCGAT 1 cut(s) 396
BseDI CCNNGG 2 cut(s) 230, 321
BseGI GGATG 1 cut(s) 753
BseJI GATNNNNATC 3 cut(s) 69, 153, 159
BseLI CCNNNNNNNGG 2 cut(s) 151, 597
BseMII CTCAG 1 cut(s) 642
BseXI GCAGC 2 cut(s) 368, 779
BsgI GTGCAG 1 cut(s) 375
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 2 cut(s) 214, 432
BshVI ATCGAT 1 cut(s) 396
BsiHKAI GWGCWC 1 cut(s) 278
BsiSI CCGG 7 cut(s) 146, 152, 158, 230, 279, 321, 373
BslI CCNNNNNNNGG 2 cut(s) 151, 597
BsmAI GTCTC 3 cut(s) 348, 436, 471
BsmI GAATGC 1 cut(s) 257
BsnI GGCC 2 cut(s) 214, 432
Bso31I GGTCTC 1 cut(s) 436
Bsp1286I GDGCHC 1 cut(s) 278
Bsp13I TCCGGA 2 cut(s) 151, 157
Bsp143I GATC 8 cut(s) 64, 148, 154, 160, 200, 393, 526, 568
BspACI CCGC 6 cut(s) 102, 269, 303, 457, 506, 509
BspANI GGCC 2 cut(s) 214, 432
BspCNI CTCAG 1 cut(s) 643
BspDI ATCGAT 1 cut(s) 396
BspEI TCCGGA 2 cut(s) 151, 157
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 3 cut(s) 144, 150, 156
BspPI GGATC 7 cut(s) 143, 149, 156, 162, 168, 534, 576
BspTNI GGTCTC 1 cut(s) 436
BssECI CCNNGG 2 cut(s) 230, 321
BssMI GATC 8 cut(s) 64, 148, 154, 160, 200, 393, 526, 568
Bst4CI ACNGT 3 cut(s) 299, 337, 600
Bst6I CTCTTC 1 cut(s) 351
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 1 cut(s) 274
BstDEI CTNAG 1 cut(s) 651
BstF5I GGATG 1 cut(s) 753
BstFNI CGCG 1 cut(s) 269
BstKTI GATC 8 cut(s) 67, 151, 157, 163, 203, 396, 529, 571
BstMAI GTCTC 3 cut(s) 348, 436, 471
BstMBI GATC 8 cut(s) 64, 148, 154, 160, 200, 393, 526, 568
BstMWI GCNNNNNNNGC 1 cut(s) 375
BstSCI CCNGG 3 cut(s) 144, 229, 320
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 2 cut(s) 368, 779
BstV2I GAAGAC 2 cut(s) 68, 309
BstX2I RGATCY 4 cut(s) 148, 154, 160, 526
BstYI RGATCY 4 cut(s) 148, 154, 160, 526
Bsu15I ATCGAT 1 cut(s) 396
BsuRI GGCC 2 cut(s) 214, 432
BsuTUI ATCGAT 1 cut(s) 396
BtsCI GGATG 1 cut(s) 753
Cac8I GCNNGC 1 cut(s) 274
Cfr13I GGNCC 1 cut(s) 142
ClaI ATCGAT 1 cut(s) 396
Csp6I GTAC 1 cut(s) 85
CviAII CATG 1 cut(s) 191
CviJI RGCY 8 cut(s) 59, 214, 276, 359, 378, 426, 432, 456
CviKI_1 RGCY 8 cut(s) 59, 214, 276, 359, 378, 426, 432, 456
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 651
DpnI GATC 8 cut(s) 66, 150, 156, 162, 202, 395, 528, 570
DpnII GATC 8 cut(s) 64, 148, 154, 160, 200, 393, 526, 568
DraI TTTAAA 1 cut(s) 739
DriI GACNNNNNGTC 1 cut(s) 290
EaeI YGGCCR 1 cut(s) 430
Eam1104I CTCTTC 1 cut(s) 351
Eam1105I GACNNNNNGTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 351
EciI GGCGGA 1 cut(s) 524
Ecl136II GAGCTC 1 cut(s) 276
Eco147I AGGCCT 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 278
Eco31I GGTCTC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 142
Eco53kI GAGCTC 1 cut(s) 276
Eco72I CACGTG 1 cut(s) 463
EcoICRI GAGCTC 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 278
FaeI CATG 1 cut(s) 194
FaiI YATR 7 cut(s) 185, 192, 205, 246, 314, 384, 561
FatI CATG 1 cut(s) 190
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 6 cut(s) 270, 304, 357, 457, 507, 768
FokI GGATG 1 cut(s) 760
FriOI GRGCYC 1 cut(s) 278
Fsp4HI GCNGC 6 cut(s) 270, 304, 357, 457, 507, 768
FspBI CTAG 3 cut(s) 62, 210, 680
GluI GCNGC 6 cut(s) 270, 304, 357, 457, 507, 768
HaeIII GGCC 2 cut(s) 214, 432
HapII CCGG 7 cut(s) 146, 152, 158, 230, 279, 321, 373
Hin1II CATG 1 cut(s) 194
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 4 cut(s) 262, 341, 512, 697
HpaII CCGG 7 cut(s) 146, 152, 158, 230, 279, 321, 373
Hpy166II GTNNAC 3 cut(s) 87, 284, 295
Hpy188I TCNGA 3 cut(s) 142, 452, 471
Hpy188III TCNNGA 5 cut(s) 62, 152, 158, 164, 710
Hpy8I GTNNAC 3 cut(s) 87, 284, 295
Hpy99I CGWCG 2 cut(s) 299, 523
HpyAV CCTTC 4 cut(s) 229, 323, 622, 772
HpyCH4III ACNGT 3 cut(s) 299, 337, 600
HpyCH4IV ACGT 2 cut(s) 288, 462
HpyCH4V TGCA 2 cut(s) 356, 751
HpyF10VI GCNNNNNNNGC 1 cut(s) 375
HpyF3I CTNAG 1 cut(s) 651
HpySE526I ACGT 2 cut(s) 288, 462
Hsp92II CATG 1 cut(s) 194
Kpn2I TCCGGA 2 cut(s) 151, 157
Kzo9I GATC 8 cut(s) 64, 148, 154, 160, 200, 393, 526, 568
LmnI GCTCC 3 cut(s) 199, 281, 375
LpnPI CCDG 7 cut(s) 159, 165, 171, 243, 292, 334, 386
Lsp1109I GCAGC 2 cut(s) 368, 779
LweI GCATC 1 cut(s) 738
MaeI CTAG 3 cut(s) 62, 210, 680
MaeII ACGT 2 cut(s) 288, 462
MalI GATC 8 cut(s) 66, 150, 156, 162, 202, 395, 528, 570
MboI GATC 8 cut(s) 64, 148, 154, 160, 200, 393, 526, 568
MflI RGATCY 4 cut(s) 148, 154, 160, 526
MhlI GDGCHC 1 cut(s) 278
MlsI TGGCCA 1 cut(s) 432
MluCI AATT 2 cut(s) 177, 415
MluNI TGGCCA 1 cut(s) 432
MlyI GAGTC 2 cut(s) 350, 521
MmeI TCCRAC 2 cut(s) 120, 644
MnlI CCTC 3 cut(s) 90, 225, 517
Mox20I TGGCCA 1 cut(s) 432
MroI TCCGGA 2 cut(s) 151, 157
MscI TGGCCA 1 cut(s) 432
MseI TTAA 4 cut(s) 615, 717, 723, 738
Msp20I TGGCCA 1 cut(s) 432
MspI CCGG 7 cut(s) 146, 152, 158, 230, 279, 321, 373
MspR9I CCNGG 3 cut(s) 146, 231, 322
Mva1269I GAATGC 1 cut(s) 257
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 1 cut(s) 375
NciI CCSGG 3 cut(s) 146, 231, 322
NdeII GATC 8 cut(s) 64, 148, 154, 160, 200, 393, 526, 568
NlaIII CATG 1 cut(s) 194
NlaIV GGNNCC 3 cut(s) 144, 150, 156
PceI AGGCCT 1 cut(s) 214
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 706
PctI GAATGC 1 cut(s) 257
PfeI GAWTC 2 cut(s) 262, 697
PkrI GCNGC 6 cut(s) 271, 305, 358, 458, 508, 769
PleI GAGTC 2 cut(s) 349, 520
PmaCI CACGTG 1 cut(s) 463
PmlI CACGTG 1 cut(s) 463
PpsI GAGTC 2 cut(s) 349, 520
Ppu21I YACGTR 1 cut(s) 463
Psp124BI GAGCTC 1 cut(s) 278
PspCI CACGTG 1 cut(s) 463
PspN4I GGNNCC 3 cut(s) 144, 150, 156
PspPI GGNCC 1 cut(s) 142
PsuI RGATCY 4 cut(s) 148, 154, 160, 526
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SacI GAGCTC 1 cut(s) 278
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 4 cut(s) 615, 717, 723, 738
SatI GCNGC 6 cut(s) 270, 304, 357, 457, 507, 768
Sau3AI GATC 8 cut(s) 64, 148, 154, 160, 200, 393, 526, 568
Sau96I GGNCC 1 cut(s) 142
SchI GAGTC 2 cut(s) 350, 521
ScrFI CCNGG 3 cut(s) 146, 231, 322
SduI GDGCHC 1 cut(s) 278
SetI ASST 8 cut(s) 61, 245, 278, 291, 361, 380, 428, 465
SfaNI GCATC 1 cut(s) 738
SinI GGWCC 1 cut(s) 142
Sse9I AATT 2 cut(s) 177, 415
SseBI AGGCCT 1 cut(s) 214
SsiI CCGC 6 cut(s) 102, 269, 303, 457, 506, 509
SspMI CTAG 3 cut(s) 62, 210, 680
SstI GAGCTC 1 cut(s) 278
StuI AGGCCT 1 cut(s) 214
StyD4I CCNGG 3 cut(s) 144, 229, 320
TaaI ACNGT 3 cut(s) 299, 337, 600
TaiI ACGT 2 cut(s) 291, 465
TaqI TCGA 3 cut(s) 294, 396, 518
TasI AATT 2 cut(s) 177, 415
TauI GCSGC 4 cut(s) 272, 306, 459, 509
TfiI GAWTC 2 cut(s) 262, 697
Tru1I TTAA 4 cut(s) 615, 717, 723, 738
Tru9I TTAA 4 cut(s) 615, 717, 723, 738
TseI GCWGC 2 cut(s) 356, 767
TspDTI ATGAA 4 cut(s) 530, 566, 633, 705
TspGWI ACGGA 2 cut(s) 72, 536
VpaK11BI GGWCC 1 cut(s) 142
XbaI TCTAGA 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 294
XspI CTAG 3 cut(s) 62, 210, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.