Rh6DG415300

Zinc-finger homeodomain protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008478
Physical Location & Seq
Forward (+)
59953922 .. 59955941
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG415300.1

Sequence Viewer

Length: 1041 bp
ATGAGAACGCAAGACAATGAAATGAGGACGTCAGGCTCTGCTTTGAGCTATGGTGGCACTCATTTAACGAAGGAATCATCGGGAGGTGGAGAAAAAAGAAGACTTGGAAATATTCACAATGGCACTCCTACTGCCCTCTCTGCTTTCACTGCTACTCACCAAACCCTAGACCACCATCACCCTCTTCAACCTCACCATGTTCAACCAGTGCCACTAACAACCAGAGATCTAGATCCAGATCGAGTCGGCTCCACTGCAGTTGCACCAAGTGGTGGTGTTTTGTCGGGTGGGCCCAAGTCCAAGACACCATCGTCGACATCCACAACCAGCAACGTCCGCTACCGAGAATGTCTCAAGAACCACGCAGCCAACATCGGCGGTAATGTGTTCGACGGGTGCGGAGAGTTCATGCCTTGCGGCGAGGAAGGAACCCTCGAAGCTCTGAAATGCGCGGCTTGTGATTGCCACAGGAACTTCCACCGTAAGGAGGTGGACGGCGAGACGACGCCGTTCGGGCCCGGCTCCAGATCAAGAAGCATAATGCTGAGCCCTCTCCAGCTCCCTCCGCCGCTTCCGTCTCGGTCCTCGGCTCTCCACCACCACCGCCACCAGAAATTCTCGATTGTTCAACCCATGAGCGTGGCTTTCGGTGGGGGAGGAGGGGGGACGGAGTCTTCGAGTGAGGATCTGAACGTTTTCGACAATGGGGATGGAGTAGGAGGAGGGGTGGTGCCGCCCTTTAGCTTGTCGAAGAAGCGGTTCAGGACCAAGTTCACGGCGGAGCAGAAGGTGAAGATGGTGGAGTTTGCGGAGCGAGTCGGGTGGAGAATTCAGAAGCAGGATGAGGAGGATGTGGAGAAGTTTTGTGATGAGGTGGGAGTGAAGAGGCAGGTGCTGAGGGTATGGATGCACAATAACAAGAACACAGTGAAGAAGACGCAGCATGACCATGACATCACCACCACCACAAGTCAGCTGGCATTGGAGAATGTTGAAGCTCATGAAGAACCAGGTCAAGAAGGAGGAGGGACAGACACATAA

Protein Analysis

346

Amino Acids

37.46

Weight (kDa)

6.83

Isoelectric Point (pI)

59.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZF-HD_dimer PF04770 111 - 164 2.7e-29 ZF-HD protein dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 880
AasI GACNNNNNNGTC 1 cut(s) 310
AatII GACGTC 1 cut(s) 32
Acc36I ACCTGC 1 cut(s) 880
AccB1I GGYRCC 1 cut(s) 730
AccB7I CCANNNNNTGG 1 cut(s) 272
AccI GTMKAC 1 cut(s) 314
AccII CGCG 1 cut(s) 452
AclI AACGTT 1 cut(s) 693
AclWI GGATC 2 cut(s) 227, 693
AcsI RAATTY 2 cut(s) 614, 828
AcyI GRCGYC 2 cut(s) 29, 506
AdeI CACNNNGTG 1 cut(s) 269
AfiI CCNNNNNNNGG 3 cut(s) 272, 484, 487
AgsI TTSAA 4 cut(s) 188, 203, 629, 995
AjnI CCWGG 1 cut(s) 1009
AluBI AGCT 6 cut(s) 48, 440, 559, 744, 976, 998
AluI AGCT 6 cut(s) 48, 440, 559, 744, 976, 998
Alw26I GTCTC 3 cut(s) 356, 494, 582
AlwI GGATC 2 cut(s) 227, 693
AlwNI CAGNNNCTG 2 cut(s) 38, 895
AoxI GGCC 2 cut(s) 290, 515
ApaI GGGCCC 2 cut(s) 294, 519
ApeKI GCWGC 2 cut(s) 365, 940
ApoI RAATTY 2 cut(s) 614, 828
ArsI GACNNNNNNTTYG 2 cut(s) 658, 690
Asp700I GAANNNNTTC 2 cut(s) 695, 758
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 6 cut(s) 290, 291, 515, 516, 582, 765
AsuC2I CCSGG 1 cut(s) 519
AsuHPI GGTGA 5 cut(s) 149, 170, 185, 802, 949
AvaII GGWCC 2 cut(s) 582, 765
BaeGI GKGCMC 2 cut(s) 294, 519
BanI GGYRCC 1 cut(s) 730
BanII GRGCYC 3 cut(s) 294, 519, 551
BbsI GAAGAC 3 cut(s) 106, 666, 941
BbvCI CCTCAGC 1 cut(s) 896
BbvI GCAGC 2 cut(s) 377, 952
BccI CCATC 4 cut(s) 183, 316, 704, 790
BceAI ACGGC 3 cut(s) 493, 511, 792
BciT130I CCWGG 1 cut(s) 1011
BcnI CCSGG 1 cut(s) 519
BcoDI GTCTC 3 cut(s) 356, 494, 582
BfaI CTAG 2 cut(s) 167, 230
BfmI CTRYAG 1 cut(s) 255
BfuAI ACCTGC 1 cut(s) 880
BglI GCCNNNNNGGC 1 cut(s) 514
BglII AGATCT 1 cut(s) 226
BisI GCNGC 6 cut(s) 366, 418, 453, 569, 734, 941
BlpI GCTNAGC 1 cut(s) 545
BlsI GCNGC 6 cut(s) 367, 419, 454, 570, 735, 942
Bme1390I CCNGG 2 cut(s) 519, 1011
Bme18I GGWCC 2 cut(s) 582, 765
BmgT120I GGNCC 6 cut(s) 290, 291, 515, 516, 582, 765
BmiI GGNNCC 6 cut(s) 250, 292, 430, 517, 523, 732
BmrFI CCNGG 2 cut(s) 519, 1011
BmsI GCATC 1 cut(s) 897
BpiI GAAGAC 3 cut(s) 106, 666, 941
BplI GAGNNNNNCTC 2 cut(s) 336, 368
BpmI CTGGAG 2 cut(s) 508, 539
Bpu10I CCTNAGC 1 cut(s) 896
Bpu1102I GCTNAGC 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 338
BpuMI CCSGG 1 cut(s) 519
BsaBI GATNNNNATC 2 cut(s) 231, 237
BsaHI GRCGYC 2 cut(s) 29, 506
BsaJI CCNNGG 1 cut(s) 585
BsaXI ACNNNNNCTCC 4 cut(s) 711, 714, 741, 744
Bsc4I CCNNNNNNNGG 3 cut(s) 272, 484, 487
Bse1I ACTGG 1 cut(s) 206
Bse8I GATNNNNATC 2 cut(s) 231, 237
BseBI CCWGG 1 cut(s) 1011
BseDI CCNNGG 1 cut(s) 585
BseGI GGATG 5 cut(s) 317, 715, 847, 856, 912
BseJI GATNNNNATC 2 cut(s) 231, 237
BseLI CCNNNNNNNGG 3 cut(s) 272, 484, 487
BseMII CTCAG 2 cut(s) 536, 887
BseNI ACTGG 1 cut(s) 206
BseRI GAGGAG 4 cut(s) 672, 735, 860, 1038
BseSI GKGCMC 2 cut(s) 294, 519
BseXI GCAGC 2 cut(s) 377, 952
Bsh1236I CGCG 1 cut(s) 452
BshFI GGCC 2 cut(s) 292, 517
BshNI GGYRCC 1 cut(s) 730
BsiSI CCGG 1 cut(s) 519
BslFI GGGAC 1 cut(s) 679
BslI CCNNNNNNNGG 3 cut(s) 272, 484, 487
BsmAI GTCTC 3 cut(s) 356, 494, 582
BsmBI CGTCTC 2 cut(s) 494, 582
BsmFI GGGAC 1 cut(s) 679
BsnI GGCC 2 cut(s) 292, 517
Bsp120I GGGCCC 2 cut(s) 290, 515
Bsp1286I GDGCHC 3 cut(s) 294, 519, 551
Bsp143I GATC 5 cut(s) 226, 232, 238, 527, 685
Bsp1720I GCTNAGC 1 cut(s) 545
BspANI GGCC 2 cut(s) 292, 517
BspCNI CTCAG 2 cut(s) 537, 888
BspFNI CGCG 1 cut(s) 452
BspHI TCATGA 1 cut(s) 1000
BspLI GGNNCC 6 cut(s) 250, 292, 430, 517, 523, 732
BspMAI CTGCAG 1 cut(s) 259
BspMI ACCTGC 1 cut(s) 880
BspPI GGATC 2 cut(s) 227, 693
BspT107I GGYRCC 1 cut(s) 730
BsrI ACTGG 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 585
BssMI GATC 5 cut(s) 226, 232, 238, 527, 685
BssNI GRCGYC 2 cut(s) 29, 506
Bst2UI CCWGG 1 cut(s) 1011
Bst4CI ACNGT 2 cut(s) 482, 928
Bst6I CTCTTC 2 cut(s) 189, 878
BstACI GRCGYC 2 cut(s) 29, 506
BstC8I GCNNGC 1 cut(s) 978
BstDEI CTNAG 2 cut(s) 545, 896
BstF5I GGATG 5 cut(s) 317, 715, 847, 856, 912
BstFNI CGCG 1 cut(s) 452
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 5 cut(s) 229, 235, 241, 530, 688
BstMAI GTCTC 3 cut(s) 356, 494, 582
BstMBI GATC 5 cut(s) 226, 232, 238, 527, 685
BstMWI GCNNNNNNNGC 6 cut(s) 54, 140, 149, 336, 514, 565
BstNI CCWGG 1 cut(s) 1011
BstSCI CCNGG 2 cut(s) 517, 1009
BstSFI CTRYAG 1 cut(s) 255
BstSLI GKGCMC 2 cut(s) 294, 519
BstUI CGCG 1 cut(s) 452
BstV1I GCAGC 2 cut(s) 377, 952
BstV2I GAAGAC 3 cut(s) 106, 666, 941
BstX2I RGATCY 3 cut(s) 226, 232, 685
BstXI CCANNNNNNTGG 1 cut(s) 640
BstYI RGATCY 3 cut(s) 226, 232, 685
BsuRI GGCC 2 cut(s) 292, 517
BtsCI GGATG 5 cut(s) 317, 715, 847, 856, 912
BtsI GCAGTG 2 cut(s) 147, 252
BtsIMutI CAGTG 4 cut(s) 147, 213, 252, 933
BveI ACCTGC 1 cut(s) 880
Cac8I GCNNGC 1 cut(s) 978
CaiI CAGNNNCTG 2 cut(s) 38, 895
CciI TCATGA 1 cut(s) 1000
CfoI GCGC 1 cut(s) 452
Cfr13I GGNCC 6 cut(s) 290, 291, 515, 516, 582, 765
CseI GACGC 2 cut(s) 514, 946
CsiI ACCWGGT 1 cut(s) 1009
CspCI CAANNNNNGTGG 2 cut(s) 241, 276
CviAII CATG 6 cut(s) 197, 409, 634, 944, 950, 1001
DdeI CTNAG 2 cut(s) 545, 896
DpnI GATC 5 cut(s) 228, 234, 240, 529, 687
DpnII GATC 5 cut(s) 226, 232, 238, 527, 685
DraIII CACNNNGTG 1 cut(s) 269
DrdI GACNNNNNNGTC 1 cut(s) 310
DseDI GACNNNNNNGTC 1 cut(s) 310
Eam1104I CTCTTC 2 cut(s) 189, 878
EarI CTCTTC 2 cut(s) 189, 878
EciI GGCGGA 2 cut(s) 555, 794
Eco24I GRGCYC 3 cut(s) 294, 519, 551
Eco47I GGWCC 2 cut(s) 582, 765
EcoRI GAATTC 1 cut(s) 828
EcoRII CCWGG 1 cut(s) 1009
EcoT38I GRGCYC 3 cut(s) 294, 519, 551
Esp3I CGTCTC 2 cut(s) 494, 582
FaeI CATG 6 cut(s) 200, 412, 637, 947, 953, 1004
FaqI GGGAC 1 cut(s) 679
FatI CATG 6 cut(s) 196, 408, 633, 943, 949, 1000
FblI GTMKAC 1 cut(s) 314
Fnu4HI GCNGC 6 cut(s) 366, 418, 453, 569, 734, 941
FokI GGATG 5 cut(s) 304, 722, 854, 863, 919
FriOI GRGCYC 3 cut(s) 294, 519, 551
Fsp4HI GCNGC 6 cut(s) 366, 418, 453, 569, 734, 941
FspBI CTAG 2 cut(s) 167, 230
GlaI GCGC 1 cut(s) 451
GluI GCNGC 6 cut(s) 366, 418, 453, 569, 734, 941
GsuI CTGGAG 2 cut(s) 508, 539
HaeIII GGCC 2 cut(s) 292, 517
HapII CCGG 1 cut(s) 519
HgaI GACGC 2 cut(s) 514, 946
HhaI GCGC 1 cut(s) 452
Hin1I GRCGYC 2 cut(s) 29, 506
Hin1II CATG 6 cut(s) 200, 412, 637, 947, 953, 1004
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HincII GTYRAC 1 cut(s) 315
HindII GTYRAC 1 cut(s) 315
HinfI GANTC 4 cut(s) 74, 243, 671, 816
HpaII CCGG 1 cut(s) 519
HphI GGTGA 5 cut(s) 149, 170, 185, 802, 949
Hpy166II GTNNAC 3 cut(s) 315, 493, 774
Hpy188I TCNGA 3 cut(s) 444, 690, 834
Hpy8I GTNNAC 3 cut(s) 315, 493, 774
Hpy99I CGWCG 3 cut(s) 316, 395, 508
HpyAV CCTTC 4 cut(s) 64, 419, 781, 1013
HpyCH4III ACNGT 2 cut(s) 482, 928
HpyCH4IV ACGT 3 cut(s) 29, 333, 693
HpyCH4V TGCA 3 cut(s) 257, 263, 910
HpyF10VI GCNNNNNNNGC 6 cut(s) 54, 140, 149, 336, 514, 565
HpyF3I CTNAG 2 cut(s) 545, 896
HpySE526I ACGT 3 cut(s) 29, 333, 693
Hsp92I GRCGYC 2 cut(s) 29, 506
Hsp92II CATG 6 cut(s) 200, 412, 637, 947, 953, 1004
HspAI GCGC 1 cut(s) 450
Kzo9I GATC 5 cut(s) 226, 232, 238, 527, 685
LmnI GCTCC 5 cut(s) 254, 527, 564, 781, 811
Lsp1109I GCAGC 2 cut(s) 377, 952
LweI GCATC 1 cut(s) 897
MabI ACCWGGT 1 cut(s) 1009
MaeI CTAG 2 cut(s) 167, 230
MaeII ACGT 3 cut(s) 29, 333, 693
MalI GATC 5 cut(s) 228, 234, 240, 529, 687
MboI GATC 5 cut(s) 226, 232, 238, 527, 685
MboII GAAGA 9 cut(s) 111, 176, 666, 763, 805, 895, 943, 946, 1016
MflI RGATCY 3 cut(s) 226, 232, 685
MhlI GDGCHC 3 cut(s) 294, 519, 551
MluCI AATT 2 cut(s) 614, 828
MlyI GAGTC 3 cut(s) 252, 680, 825
MroXI GAANNNNTTC 2 cut(s) 695, 758
MseI TTAA 1 cut(s) 65
MslI CAYNNNNRTG 2 cut(s) 638, 948
MspA1I CMGCKG 1 cut(s) 976
MspI CCGG 1 cut(s) 519
MspR9I CCNGG 2 cut(s) 519, 1011
MvaI CCWGG 1 cut(s) 1011
MvnI CGCG 1 cut(s) 452
MwoI GCNNNNNNNGC 6 cut(s) 54, 140, 149, 336, 514, 565
NciI CCSGG 1 cut(s) 519
NdeII GATC 5 cut(s) 226, 232, 238, 527, 685
NlaIII CATG 6 cut(s) 200, 412, 637, 947, 953, 1004
NlaIV GGNNCC 6 cut(s) 250, 292, 430, 517, 523, 732
NmeAIII GCCGAG 1 cut(s) 566
PagI TCATGA 1 cut(s) 1000
PaqCI CACCTGC 1 cut(s) 880
PcsI WCGNNNNNNNCGW 1 cut(s) 674
PdmI GAANNNNTTC 2 cut(s) 695, 758
PfeI GAWTC 1 cut(s) 74
PflFI GACNNNGTC 1 cut(s) 670
PflMI CCANNNNNTGG 1 cut(s) 272
PkrI GCNGC 6 cut(s) 367, 419, 454, 570, 735, 942
PleI GAGTC 3 cut(s) 251, 679, 824
PpsI GAGTC 3 cut(s) 251, 679, 824
Psp1406I AACGTT 1 cut(s) 693
Psp6I CCWGG 1 cut(s) 1009
PspGI CCWGG 1 cut(s) 1009
PspN4I GGNNCC 6 cut(s) 250, 292, 430, 517, 523, 732
PspOMI GGGCCC 2 cut(s) 290, 515
PspPI GGNCC 6 cut(s) 290, 291, 515, 516, 582, 765
PstI CTGCAG 1 cut(s) 259
PstNI CAGNNNCTG 2 cut(s) 38, 895
PsuI RGATCY 3 cut(s) 226, 232, 685
PsyI GACNNNGTC 1 cut(s) 670
PvuII CAGCTG 1 cut(s) 976
RseI CAYNNNNRTG 2 cut(s) 638, 948
SalI GTCGAC 1 cut(s) 313
SaqAI TTAA 1 cut(s) 65
SatI GCNGC 6 cut(s) 366, 418, 453, 569, 734, 941
Sau3AI GATC 5 cut(s) 226, 232, 238, 527, 685
Sau96I GGNCC 6 cut(s) 290, 291, 515, 516, 582, 765
SchI GAGTC 3 cut(s) 252, 680, 825
ScrFI CCNGG 2 cut(s) 519, 1011
SduI GDGCHC 3 cut(s) 294, 519, 551
SexAI ACCWGGT 1 cut(s) 1009
SfaNI GCATC 1 cut(s) 897
SfcI CTRYAG 1 cut(s) 255
SinI GGWCC 2 cut(s) 582, 765
SmiMI CAYNNNNRTG 2 cut(s) 638, 948
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 2 cut(s) 614, 828
SspI AATATT 1 cut(s) 112
SspMI CTAG 2 cut(s) 167, 230
StyD4I CCNGG 2 cut(s) 517, 1009
TaaI ACNGT 2 cut(s) 482, 928
TaiI ACGT 3 cut(s) 32, 336, 696
TaqI TCGA 8 cut(s) 241, 314, 390, 435, 620, 677, 699, 749
TaqII GACCGA 1 cut(s) 570
TasI AATT 2 cut(s) 614, 828
TauI GCSGC 4 cut(s) 420, 455, 571, 736
TfiI GAWTC 1 cut(s) 74
Tru1I TTAA 1 cut(s) 65
Tru9I TTAA 1 cut(s) 65
TscAI CASTG 4 cut(s) 154, 213, 259, 933
TseI GCWGC 2 cut(s) 365, 940
TspDTI ATGAA 3 cut(s) 33, 397, 1017
TspGWI ACGGA 2 cut(s) 564, 683
TspRI CASTG 4 cut(s) 154, 213, 259, 933
Tth111I GACNNNGTC 1 cut(s) 670
Van91I CCANNNNNTGG 1 cut(s) 272
VpaK11BI GGWCC 2 cut(s) 582, 765
XapI RAATTY 2 cut(s) 614, 828
XbaI TCTAGA 1 cut(s) 229
XcmI CCANNNNNNNNNTGG 1 cut(s) 973
XmiI GTMKAC 1 cut(s) 314
XmnI GAANNNNTTC 2 cut(s) 695, 758
XspI CTAG 2 cut(s) 167, 230
ZraI GACGTC 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.