pycom08g02410

Zinc-finger homeodomain protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008478
Physical Location & Seq
Reverse (-)
1771764 .. 1772813
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g02410.1

Sequence Viewer

Length: 1050 bp
ATGGATTTTAGGGCACAAGATAACGAAATGAGGACGTCAGGCTCGCTAAGCTATGCTCATTTAATTACCAAAGAATCATCGCCGTCGCCAGCGGCAGCAGCAGATCATCAGCAAAAGAGAAGAGTTGGGATTCACAATGGCACTGGGAATTACGGTTCTGCCACTGCTCCTCATCACCAGCACCAAACCCTAGACAACTACCACCCAGCTGCTATCCCGTTGCCGCCGTTGATCCGCGACCCAGATCCGGATCGTGCCCTATCCGGGACCCCGGTGGCCCCACAGGGAGCCGGAGTCGGTGGCGGTGGCGGTGGTGGGGGGCCCAAGTCGTTGGCAAAAGTTGTTAGGTATAGAGAGTGCCTCAAGAACCACGCGGCGAACATTGGTGGTAATGTGTTCGACGGCTGCGGAGAGTTCATGCCGAGCGGCGAGGAGGGGACGATGGAGGCTTTGAAATGTGCGGCGTGCGACTGCCACCGCAACTTTCACCGCAAGGAGGTGGACGGGGAGACGGCGGCCTTCAGTCCCGGGTCGAGAAGGTCGAGCATAATGTTGAGTCCGCTTCAGCTTCCGCCGCCGCTGCCTTCGCCTTCCTCGGCTTTGCACCATCACCACCACCACCACCACCATCAGAAGTACGCGATGCCTCCGATTGTCCAGCCTGTGAACGTGGCGTTCGGGAGCAGCGGAGGAGGGACGGAGAGTTCGAGCGAGGATCTGAATGCTTTTGAGGGTGGAACTGTCCCGCCGTTTGCTTCGTCTAAGAAGCGGTTTCGGACAAAGTTCACGGTGGAGCAGAAGGAGAGGATGATGGAGTTTGGGGAGAAGGTTGGGTGGAGAATTCAGAAGCAGGATGAGGAGGAGGTGGAGAGGTTTTGTGCGGAGGTGGGAGTGAAGAGGCAGGTGCTTAGGGTTTGGATGCATAACAACAAAAACACCATTAAGAAGCAGGGTGACGTCGCTGCTACTACTAATGCCATAGCTACAGGTCTGTCTTTTAAGAACGTGGAGGGAGAAGGAGGGGAGGGAGGGGCAGCAGCTGAAGATTAA

Protein Analysis

350

Amino Acids

37.46

Weight (kDa)

7.06

Isoelectric Point (pI)

60.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZF-HD_dimer PF04770 115 - 167 8.8e-31 ZF-HD protein dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 892
AatII GACGTC 2 cut(s) 38, 960
Acc36I ACCTGC 1 cut(s) 892
AccBSI CCGCTC 1 cut(s) 426
AccII CGCG 3 cut(s) 237, 374, 641
AccIII TCCGGA 1 cut(s) 247
AclWI GGATC 4 cut(s) 226, 239, 258, 723
AcsI RAATTY 1 cut(s) 840
AcuI CTGAAG 2 cut(s) 505, 548
AcyI GRCGYC 2 cut(s) 35, 957
AfaI GTAC 1 cut(s) 638
AfiI CCNNNNNNNGG 3 cut(s) 247, 264, 496
AgsI TTSAA 1 cut(s) 454
AloI GAACNNNNNNTCC 4 cut(s) 139, 171, 688, 720
AluBI AGCT 5 cut(s) 51, 209, 568, 983, 1040
AluI AGCT 5 cut(s) 51, 209, 568, 983, 1040
Alw26I GTCTC 1 cut(s) 503
AlwI GGATC 4 cut(s) 226, 239, 258, 723
AlwNI CAGNNNCTG 1 cut(s) 1040
Ama87I CYCGRG 1 cut(s) 527
Aor13HI TCCGGA 1 cut(s) 247
AoxI GGCC 3 cut(s) 276, 320, 516
ApaI GGGCCC 1 cut(s) 324
ApeKI GCWGC 9 cut(s) 95, 98, 209, 405, 580, 684, 962, 1034, 1037
ApoI RAATTY 1 cut(s) 840
ArsI GACNNNNNNTTYG 2 cut(s) 688, 720
AspS9I GGNCC 4 cut(s) 267, 277, 320, 321
AsuC2I CCSGG 4 cut(s) 265, 272, 528, 529
AsuHPI GGTGA 4 cut(s) 167, 479, 602, 965
AvaI CYCGRG 1 cut(s) 527
AvaII GGWCC 1 cut(s) 267
BaeGI GKGCMC 3 cut(s) 16, 259, 324
BanII GRGCYC 1 cut(s) 324
BbvI GCAGC 8 cut(s) 107, 110, 196, 392, 567, 696, 949, 1046
BccI CCATC 4 cut(s) 436, 615, 636, 805
BceAI ACGGC 5 cut(s) 67, 211, 418, 528, 733
BcnI CCSGG 4 cut(s) 265, 272, 528, 529
BcoDI GTCTC 1 cut(s) 503
BfaI CTAG 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 984
BfuAI ACCTGC 1 cut(s) 892
BlpI GCTNAGC 1 cut(s) 47
Bme1390I CCNGG 4 cut(s) 265, 272, 528, 529
Bme18I GGWCC 1 cut(s) 267
BmeT110I CYCGRG 1 cut(s) 527
BmgT120I GGNCC 4 cut(s) 267, 277, 320, 321
BmiI GGNNCC 6 cut(s) 268, 269, 279, 289, 321, 322
BmrFI CCNGG 4 cut(s) 265, 272, 528, 529
BmrI ACTGGG 1 cut(s) 153
BmsI GCATC 2 cut(s) 633, 909
BmuI ACTGGG 1 cut(s) 153
BplI GAGNNNNNCTC 2 cut(s) 345, 377
Bpu10I CCTNAGC 1 cut(s) 908
Bpu1102I GCTNAGC 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 4 cut(s) 265, 272, 528, 529
BsaBI GATNNNNATC 1 cut(s) 249
BsaHI GRCGYC 2 cut(s) 35, 957
BsaJI CCNNGG 3 cut(s) 270, 527, 594
BsaWI WCCGGW 1 cut(s) 247
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 3 cut(s) 247, 264, 496
Bse1I ACTGG 1 cut(s) 148
Bse8I GATNNNNATC 1 cut(s) 249
BseAI TCCGGA 1 cut(s) 247
BseDI CCNNGG 3 cut(s) 270, 527, 594
BseGI GGATG 3 cut(s) 813, 859, 924
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 3 cut(s) 247, 264, 496
BseNI ACTGG 1 cut(s) 148
BseRI GAGGAG 5 cut(s) 159, 446, 705, 872, 875
BseSI GKGCMC 3 cut(s) 16, 259, 324
BseXI GCAGC 8 cut(s) 107, 110, 196, 392, 567, 696, 949, 1046
BseYI CCCAGC 1 cut(s) 205
Bsh1236I CGCG 3 cut(s) 237, 374, 641
BshFI GGCC 3 cut(s) 278, 322, 518
BsiHKCI CYCGRG 1 cut(s) 527
BsiSI CCGG 5 cut(s) 248, 264, 272, 291, 528
BslFI GGGAC 5 cut(s) 280, 451, 510, 709, 728
BslI CCNNNNNNNGG 3 cut(s) 247, 264, 496
BsmAI GTCTC 1 cut(s) 503
BsmBI CGTCTC 1 cut(s) 503
BsmFI GGGAC 5 cut(s) 280, 451, 510, 709, 728
BsmI GAATGC 1 cut(s) 727
BsnI GGCC 3 cut(s) 278, 322, 518
BsoBI CYCGRG 1 cut(s) 527
Bsp120I GGGCCC 1 cut(s) 320
Bsp1286I GDGCHC 3 cut(s) 16, 259, 324
Bsp13I TCCGGA 1 cut(s) 247
Bsp143I GATC 5 cut(s) 103, 231, 244, 250, 715
Bsp1720I GCTNAGC 1 cut(s) 47
BspANI GGCC 3 cut(s) 278, 322, 518
BspEI TCCGGA 1 cut(s) 247
BspFNI CGCG 3 cut(s) 237, 374, 641
BspLI GGNNCC 6 cut(s) 268, 269, 279, 289, 321, 322
BspMI ACCTGC 1 cut(s) 892
BspPI GGATC 4 cut(s) 226, 239, 258, 723
BsrBI CCGCTC 1 cut(s) 426
BsrI ACTGG 1 cut(s) 148
BssECI CCNNGG 3 cut(s) 270, 527, 594
BssMI GATC 5 cut(s) 103, 231, 244, 250, 715
BssNI GRCGYC 2 cut(s) 35, 957
Bst4CI ACNGT 3 cut(s) 155, 742, 790
Bst6I CTCTTC 2 cut(s) 115, 890
BstACI GRCGYC 2 cut(s) 35, 957
BstC8I GCNNGC 3 cut(s) 44, 90, 466
BstDEI CTNAG 3 cut(s) 47, 762, 908
BstF5I GGATG 3 cut(s) 813, 859, 924
BstFNI CGCG 3 cut(s) 237, 374, 641
BstKTI GATC 5 cut(s) 106, 234, 247, 253, 718
BstMAI GTCTC 1 cut(s) 503
BstMBI GATC 5 cut(s) 103, 231, 244, 250, 715
BstMWI GCNNNNNNNGC 5 cut(s) 48, 98, 574, 580, 586
BstSCI CCNGG 4 cut(s) 263, 270, 526, 527
BstSFI CTRYAG 1 cut(s) 984
BstSLI GKGCMC 3 cut(s) 16, 259, 324
BstUI CGCG 3 cut(s) 237, 374, 641
BstV1I GCAGC 8 cut(s) 107, 110, 196, 392, 567, 696, 949, 1046
BstX2I RGATCY 2 cut(s) 244, 715
BstXI CCANNNNNNTGG 1 cut(s) 331
BstYI RGATCY 2 cut(s) 244, 715
BsuRI GGCC 3 cut(s) 278, 322, 518
BtgZI GCGATG 2 cut(s) 63, 656
BtsCI GGATG 3 cut(s) 813, 859, 924
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 2 cut(s) 141, 162
BveI ACCTGC 1 cut(s) 892
Cac8I GCNNGC 3 cut(s) 44, 90, 466
CaiI CAGNNNCTG 1 cut(s) 1040
Cfr13I GGNCC 4 cut(s) 267, 277, 320, 321
Cfr9I CCCGGG 1 cut(s) 527
Csp6I GTAC 1 cut(s) 637
CviAII CATG 1 cut(s) 418
CviQI GTAC 1 cut(s) 637
DdeI CTNAG 3 cut(s) 47, 762, 908
DpnI GATC 5 cut(s) 105, 233, 246, 252, 717
DpnII GATC 5 cut(s) 103, 231, 244, 250, 715
Eam1104I CTCTTC 2 cut(s) 115, 890
EarI CTCTTC 2 cut(s) 115, 890
EciI GGCGGA 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 267
Eco57I CTGAAG 2 cut(s) 505, 548
Eco88I CYCGRG 1 cut(s) 527
EcoO109I RGGNCCY 2 cut(s) 267, 320
EcoRI GAATTC 1 cut(s) 840
EcoT22I ATGCAT 1 cut(s) 924
EcoT38I GRGCYC 1 cut(s) 324
Esp3I CGTCTC 1 cut(s) 503
FaeI CATG 1 cut(s) 421
FaiI YATR 6 cut(s) 54, 351, 419, 548, 924, 980
FaqI GGGAC 5 cut(s) 280, 451, 510, 709, 728
FatI CATG 1 cut(s) 417
FauI CCCGC 1 cut(s) 753
FokI GGATG 3 cut(s) 820, 866, 931
FriOI GRGCYC 1 cut(s) 324
FspBI CTAG 1 cut(s) 191
GsaI CCCAGC 1 cut(s) 209
HaeIII GGCC 3 cut(s) 278, 322, 518
HapII CCGG 5 cut(s) 248, 264, 272, 291, 528
Hin1I GRCGYC 2 cut(s) 35, 957
Hin1II CATG 1 cut(s) 421
HinfI GANTC 4 cut(s) 74, 130, 294, 556
HpaII CCGG 5 cut(s) 248, 264, 272, 291, 528
HphI GGTGA 4 cut(s) 167, 479, 602, 965
Hpy166II GTNNAC 3 cut(s) 502, 667, 786
Hpy188I TCNGA 5 cut(s) 633, 651, 720, 777, 846
Hpy188III TCNNGA 4 cut(s) 248, 364, 534, 679
Hpy8I GTNNAC 3 cut(s) 502, 667, 786
Hpy99I CGWCG 3 cut(s) 88, 404, 962
HpyAV CCTTC 7 cut(s) 529, 531, 594, 600, 793, 820, 1010
HpyCH4III ACNGT 3 cut(s) 155, 742, 790
HpyCH4IV ACGT 4 cut(s) 35, 669, 957, 1005
HpyCH4V TGCA 2 cut(s) 604, 922
HpyF10VI GCNNNNNNNGC 5 cut(s) 48, 98, 574, 580, 586
HpyF3I CTNAG 3 cut(s) 47, 762, 908
HpySE526I ACGT 4 cut(s) 35, 669, 957, 1005
Hsp92I GRCGYC 2 cut(s) 35, 957
Hsp92II CATG 1 cut(s) 421
KflI GGGWCCC 1 cut(s) 267
Kpn2I TCCGGA 1 cut(s) 247
Kzo9I GATC 5 cut(s) 103, 231, 244, 250, 715
LmnI GCTCC 4 cut(s) 172, 287, 681, 793
Lsp1109I GCAGC 8 cut(s) 107, 110, 196, 392, 567, 696, 949, 1046
LweI GCATC 2 cut(s) 633, 909
MaeI CTAG 1 cut(s) 191
MaeII ACGT 4 cut(s) 35, 669, 957, 1005
MaeIII GTNAC 1 cut(s) 953
MalI GATC 5 cut(s) 105, 233, 246, 252, 717
MbiI CCGCTC 1 cut(s) 426
MboI GATC 5 cut(s) 103, 231, 244, 250, 715
MboII GAAGA 2 cut(s) 132, 907
MflI RGATCY 2 cut(s) 244, 715
MhlI GDGCHC 3 cut(s) 16, 259, 324
MluCI AATT 3 cut(s) 63, 148, 840
MlyI GAGTC 2 cut(s) 303, 565
Mph1103I ATGCAT 1 cut(s) 924
MroI TCCGGA 1 cut(s) 247
MseI TTAA 4 cut(s) 62, 942, 999, 1048
MspA1I CMGCKG 5 cut(s) 92, 209, 580, 687, 1040
MspI CCGG 5 cut(s) 248, 264, 272, 291, 528
MspR9I CCNGG 4 cut(s) 265, 272, 528, 529
Mva1269I GAATGC 1 cut(s) 727
MvnI CGCG 3 cut(s) 237, 374, 641
MwoI GCNNNNNNNGC 5 cut(s) 48, 98, 574, 580, 586
NciI CCSGG 4 cut(s) 265, 272, 528, 529
NdeII GATC 5 cut(s) 103, 231, 244, 250, 715
NlaIII CATG 1 cut(s) 421
NlaIV GGNNCC 6 cut(s) 268, 269, 279, 289, 321, 322
NmeAIII GCCGAG 2 cut(s) 447, 575
NmuCI GTSAC 1 cut(s) 953
NsiI ATGCAT 1 cut(s) 924
PaqCI CACCTGC 1 cut(s) 892
PcsI WCGNNNNNNNCGW 2 cut(s) 539, 704
PctI GAATGC 1 cut(s) 727
PfeI GAWTC 2 cut(s) 74, 130
PfoI TCCNGGA 1 cut(s) 263
PleI GAGTC 2 cut(s) 302, 564
PpsI GAGTC 2 cut(s) 302, 564
PpuMI RGGWCCY 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 267
PspFI CCCAGC 1 cut(s) 205
PspN4I GGNNCC 6 cut(s) 268, 269, 279, 289, 321, 322
PspOMI GGGCCC 1 cut(s) 320
PspPI GGNCC 4 cut(s) 267, 277, 320, 321
PspPPI RGGWCCY 1 cut(s) 267
PstNI CAGNNNCTG 1 cut(s) 1040
PsuI RGATCY 2 cut(s) 244, 715
PvuII CAGCTG 2 cut(s) 209, 1040
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
SaqAI TTAA 4 cut(s) 62, 942, 999, 1048
Sau3AI GATC 5 cut(s) 103, 231, 244, 250, 715
Sau96I GGNCC 4 cut(s) 267, 277, 320, 321
SchI GAGTC 2 cut(s) 303, 565
ScrFI CCNGG 4 cut(s) 265, 272, 528, 529
SduI GDGCHC 3 cut(s) 16, 259, 324
SfaNI GCATC 2 cut(s) 633, 909
SfcI CTRYAG 1 cut(s) 984
SinI GGWCC 1 cut(s) 267
SmaI CCCGGG 1 cut(s) 529
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 3 cut(s) 63, 148, 840
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 4 cut(s) 263, 270, 526, 527
TaaI ACNGT 3 cut(s) 155, 742, 790
TaiI ACGT 4 cut(s) 38, 672, 960, 1008
TaqI TCGA 4 cut(s) 399, 533, 542, 707
TasI AATT 3 cut(s) 63, 148, 840
TauI GCSGC 8 cut(s) 95, 226, 377, 429, 464, 518, 577, 580
TfiI GAWTC 2 cut(s) 74, 130
Tru1I TTAA 4 cut(s) 62, 942, 999, 1048
Tru9I TTAA 4 cut(s) 62, 942, 999, 1048
TscAI CASTG 2 cut(s) 148, 169
TseFI GTSAC 1 cut(s) 953
TseI GCWGC 9 cut(s) 95, 98, 209, 405, 580, 684, 962, 1034, 1037
Tsp45I GTSAC 1 cut(s) 953
TspDTI ATGAA 1 cut(s) 406
TspGWI ACGGA 1 cut(s) 713
TspMI CCCGGG 1 cut(s) 527
TspRI CASTG 2 cut(s) 148, 169
VpaK11BI GGWCC 1 cut(s) 267
XapI RAATTY 1 cut(s) 840
XmaI CCCGGG 1 cut(s) 527
XspI CTAG 1 cut(s) 191
ZraI GACGTC 2 cut(s) 36, 958
Zsp2I ATGCAT 1 cut(s) 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.