MD15G1025900.v1.1

Zinc-finger homeodomain protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008478
Physical Location & Seq
Reverse (-)
1509804 .. 1511909
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1025900.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGAGGACGTCAGGCTCGCTAAGCTATGCTCATTTAAGTGCCAAAGAGTCATCGCCGTCGTCAGCGGCAGCAGCACCAGCAGCAGATCACCAGCAAAAGAGAAGACTTGGGATTCACAATGGCACTGGAAGTTACGGTTCTGCCACTGCTCCTCATCACCAGCACCAAACCCTAGACAACCACAACCCAGCTGCTCTCCCGTTGCAGCCGTTGATCCGCGACCCAGATCCGGATCGTGCCCTATCCGGCACCCCGGTGGCCCCACACGGAGCCAGACTCAGTGGCAGTGGTGGTGGTGGTGGGCCCAAGTCTTTGGGAAAAGTTGTTAGGTACAGAGAGTGCCTCAAGAACCACGCGGCGAACATTGGTGGTAATGTGTTCGACGGCTGCGGAGAGTTCATGCCGAGCGGCGAAGAGGGGACGCTGGAGGCTTTGAAATGCGCCGCGTGCGACTGCCACCGCAACTTCCACCGCAAGGAGGTGGACGGGGAGACGGCGGCTTTCAGTCCCGGGTCGAGAAGGTCGAGCATAATGCTGACTCCACTTCAACTTCCGCCGCCGCTGCCTTCGCCTTCCTCGGCTTTGCACCATCATCATCACCACCATCAGAAGTACTCGATGCCTCCGATTGTTCAGCCGGTGAACATGGCGTTCGGTAGCGGCGGAGGAGGGACGGAGAGTTCGAGCGAGGATCTGAATGCTTTTGAGGGTGGAGCGGTGCCGCCGTTTGCTTTGTCGAAGAAGCGGTTTCGAACAAAGTTTACGGTGGAGCAGAAGGAGAGAATGATGGAGTTTGCGGAGAGGGTTGGGTGGAGAATTCAGAAGCAGGATGAGGAGGAGGTGGAGAGGTTTTGTGCGGAGGTGGGAGTGAAGAGGCAGGTGCTTAGGGTTTGGATGCACAACAACAAAAACACCATTAAGAAGCAGGGTGACGTCACTGCTACTACTAATGCCATTGCTACAGGTCTGTCTTTTGACAATGTGGAGGGAGAAGGTGGGGAGGGAGCGGCAGCAGCTGAAGATTAA

Protein Analysis

342

Amino Acids

36.47

Weight (kDa)

7.33

Isoelectric Point (pI)

59.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZF-HD_dimer PF04770 109 - 161 6.6e-31 ZF-HD protein dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 868
AatII GACGTC 2 cut(s) 11, 936
Acc36I ACCTGC 1 cut(s) 868
AccB1I GGYRCC 2 cut(s) 248, 718
AccBSI CCGCTC 3 cut(s) 408, 716, 1007
AccII CGCG 3 cut(s) 219, 356, 446
AccIII TCCGGA 1 cut(s) 229
AclWI GGATC 4 cut(s) 208, 221, 240, 699
AcsI RAATTY 1 cut(s) 816
AcyI GRCGYC 2 cut(s) 8, 933
AfaI GTAC 2 cut(s) 332, 614
AfiI CCNNNNNNNGG 3 cut(s) 229, 475, 478
AgsI TTSAA 2 cut(s) 436, 548
AleI CACNNNNGTG 1 cut(s) 254
AloI GAACNNNNNNTCC 2 cut(s) 664, 696
AluBI AGCT 3 cut(s) 24, 191, 1016
AluI AGCT 3 cut(s) 24, 191, 1016
Alw26I GTCTC 1 cut(s) 485
AlwI GGATC 4 cut(s) 208, 221, 240, 699
AlwNI CAGNNNCTG 1 cut(s) 1016
Ama87I CYCGRG 1 cut(s) 509
Aor13HI TCCGGA 1 cut(s) 229
AoxI GGCC 2 cut(s) 258, 302
ApaI GGGCCC 1 cut(s) 306
ApeKI GCWGC 9 cut(s) 68, 71, 80, 191, 205, 387, 562, 1010, 1013
ApoI RAATTY 1 cut(s) 816
ArsI GACNNNNNNTTYG 2 cut(s) 664, 696
AspLEI GCGC 1 cut(s) 443
AspS9I GGNCC 3 cut(s) 259, 302, 303
AsuC2I CCSGG 3 cut(s) 254, 510, 511
AsuHPI GGTGA 5 cut(s) 80, 149, 590, 652, 941
AsuII TTCGAA 1 cut(s) 751
AvaI CYCGRG 1 cut(s) 509
BaeGI GKGCMC 2 cut(s) 241, 306
BanI GGYRCC 2 cut(s) 248, 718
BanII GRGCYC 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 109
BbvI GCAGC 8 cut(s) 80, 83, 92, 178, 217, 374, 549, 1022
BccI CCATC 3 cut(s) 597, 612, 781
BceAI ACGGC 5 cut(s) 40, 193, 400, 510, 709
BcgI CGANNNNNNTGC 2 cut(s) 514, 548
BcnI CCSGG 3 cut(s) 254, 510, 511
BcoDI GTCTC 1 cut(s) 485
BfaI CTAG 1 cut(s) 173
BfmI CTRYAG 1 cut(s) 960
BfuAI ACCTGC 1 cut(s) 868
BlpI GCTNAGC 1 cut(s) 20
BmcAI AGTACT 1 cut(s) 614
Bme1390I CCNGG 3 cut(s) 254, 510, 511
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 3 cut(s) 259, 302, 303
BmiI GGNNCC 5 cut(s) 250, 261, 271, 304, 720
BmrFI CCNGG 3 cut(s) 254, 510, 511
BmsI GCATC 2 cut(s) 609, 885
BpiI GAAGAC 1 cut(s) 109
BplI GAGNNNNNCTC 4 cut(s) 261, 293, 327, 359
BpmI CTGGAG 1 cut(s) 446
Bpu10I CCTNAGC 1 cut(s) 884
Bpu1102I GCTNAGC 1 cut(s) 20
Bpu14I TTCGAA 1 cut(s) 751
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 3 cut(s) 254, 510, 511
BsaBI GATNNNNATC 1 cut(s) 231
BsaHI GRCGYC 2 cut(s) 8, 933
BsaJI CCNNGG 3 cut(s) 252, 509, 576
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 475, 478
Bse118I RCCGGY 1 cut(s) 637
Bse1I ACTGG 1 cut(s) 130
Bse3DI GCAATG 1 cut(s) 954
Bse8I GATNNNNATC 1 cut(s) 231
BseAI TCCGGA 1 cut(s) 229
BseDI CCNNGG 3 cut(s) 252, 509, 576
BseGI GGATG 2 cut(s) 835, 900
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 3 cut(s) 229, 475, 478
BseMI GCAATG 1 cut(s) 954
BseMII CTCAG 1 cut(s) 292
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 4 cut(s) 141, 681, 848, 851
BseSI GKGCMC 2 cut(s) 241, 306
BseXI GCAGC 8 cut(s) 80, 83, 92, 178, 217, 374, 549, 1022
BseYI CCCAGC 1 cut(s) 187
Bsh1236I CGCG 3 cut(s) 219, 356, 446
BshFI GGCC 2 cut(s) 260, 304
BshNI GGYRCC 2 cut(s) 248, 718
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 5 cut(s) 230, 246, 254, 510, 638
BslFI GGGAC 3 cut(s) 433, 492, 685
BslI CCNNNNNNNGG 3 cut(s) 229, 475, 478
BsmAI GTCTC 1 cut(s) 485
BsmBI CGTCTC 1 cut(s) 485
BsmFI GGGAC 3 cut(s) 433, 492, 685
BsmI GAATGC 1 cut(s) 703
BsnI GGCC 2 cut(s) 260, 304
BsoBI CYCGRG 1 cut(s) 509
Bsp119I TTCGAA 1 cut(s) 751
Bsp120I GGGCCC 1 cut(s) 302
Bsp1286I GDGCHC 2 cut(s) 241, 306
Bsp13I TCCGGA 1 cut(s) 229
Bsp143I GATC 5 cut(s) 85, 213, 226, 232, 691
Bsp1720I GCTNAGC 1 cut(s) 20
BspANI GGCC 2 cut(s) 260, 304
BspCNI CTCAG 1 cut(s) 291
BspEI TCCGGA 1 cut(s) 229
BspFNI CGCG 3 cut(s) 219, 356, 446
BspLI GGNNCC 5 cut(s) 250, 261, 271, 304, 720
BspMI ACCTGC 1 cut(s) 868
BspPI GGATC 4 cut(s) 208, 221, 240, 699
BspT104I TTCGAA 1 cut(s) 751
BspT107I GGYRCC 2 cut(s) 248, 718
BsrBI CCGCTC 3 cut(s) 408, 716, 1007
BsrDI GCAATG 1 cut(s) 954
BsrFI RCCGGY 1 cut(s) 637
BsrI ACTGG 1 cut(s) 130
BssAI RCCGGY 1 cut(s) 637
BssECI CCNNGG 3 cut(s) 252, 509, 576
BssMI GATC 5 cut(s) 85, 213, 226, 232, 691
BssNI GRCGYC 2 cut(s) 8, 933
Bst4CI ACNGT 2 cut(s) 137, 766
Bst6I CTCTTC 2 cut(s) 408, 866
BstACI GRCGYC 2 cut(s) 8, 933
BstBI TTCGAA 1 cut(s) 751
BstC8I GCNNGC 2 cut(s) 17, 448
BstDEI CTNAG 3 cut(s) 20, 278, 884
BstF5I GGATG 2 cut(s) 835, 900
BstFNI CGCG 3 cut(s) 219, 356, 446
BstHHI GCGC 1 cut(s) 443
BstKTI GATC 5 cut(s) 88, 216, 229, 235, 694
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 5 cut(s) 85, 213, 226, 232, 691
BstMWI GCNNNNNNNGC 8 cut(s) 21, 71, 77, 80, 447, 562, 568, 1013
BstSCI CCNGG 3 cut(s) 252, 508, 509
BstSFI CTRYAG 1 cut(s) 960
BstSLI GKGCMC 2 cut(s) 241, 306
BstUI CGCG 3 cut(s) 219, 356, 446
BstV1I GCAGC 8 cut(s) 80, 83, 92, 178, 217, 374, 549, 1022
BstV2I GAAGAC 1 cut(s) 109
BstX2I RGATCY 2 cut(s) 226, 691
BstXI CCANNNNNNTGG 1 cut(s) 313
BstYI RGATCY 2 cut(s) 226, 691
BsuRI GGCC 2 cut(s) 260, 304
BtgZI GCGATG 1 cut(s) 36
BtsCI GGATG 2 cut(s) 835, 900
BtsI GCAGTG 3 cut(s) 144, 292, 936
BtsIMutI CAGTG 5 cut(s) 123, 144, 286, 292, 936
BveI ACCTGC 1 cut(s) 868
Cac8I GCNNGC 2 cut(s) 17, 448
CaiI CAGNNNCTG 1 cut(s) 1016
CfoI GCGC 1 cut(s) 443
Cfr10I RCCGGY 1 cut(s) 637
Cfr13I GGNCC 3 cut(s) 259, 302, 303
Cfr9I CCCGGG 1 cut(s) 509
CseI GACGC 1 cut(s) 430
Csp6I GTAC 2 cut(s) 331, 613
CviAII CATG 2 cut(s) 400, 646
CviQI GTAC 2 cut(s) 331, 613
DdeI CTNAG 3 cut(s) 20, 278, 884
DpnI GATC 5 cut(s) 87, 215, 228, 234, 693
DpnII GATC 5 cut(s) 85, 213, 226, 232, 691
Eam1104I CTCTTC 2 cut(s) 408, 866
EarI CTCTTC 2 cut(s) 408, 866
EciI GGCGGA 2 cut(s) 543, 678
Eco24I GRGCYC 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 509
EcoRI GAATTC 1 cut(s) 816
EcoT38I GRGCYC 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 485
FaeI CATG 2 cut(s) 403, 649
FaiI YATR 4 cut(s) 27, 401, 530, 647
FaqI GGGAC 3 cut(s) 433, 492, 685
FatI CATG 2 cut(s) 399, 645
FokI GGATG 2 cut(s) 842, 907
FriOI GRGCYC 1 cut(s) 306
FspBI CTAG 1 cut(s) 173
GlaI GCGC 1 cut(s) 442
GsaI CCCAGC 1 cut(s) 191
GsuI CTGGAG 1 cut(s) 446
HaeIII GGCC 2 cut(s) 260, 304
HapII CCGG 5 cut(s) 230, 246, 254, 510, 638
HgaI GACGC 1 cut(s) 430
HhaI GCGC 1 cut(s) 443
Hin1I GRCGYC 2 cut(s) 8, 933
Hin1II CATG 2 cut(s) 403, 649
Hin6I GCGC 1 cut(s) 441
HinP1I GCGC 1 cut(s) 441
HinfI GANTC 4 cut(s) 47, 112, 276, 538
HpaII CCGG 5 cut(s) 230, 246, 254, 510, 638
HphI GGTGA 5 cut(s) 80, 149, 590, 652, 941
Hpy166II GTNNAC 3 cut(s) 484, 643, 762
Hpy188I TCNGA 4 cut(s) 609, 627, 696, 822
Hpy188III TCNNGA 3 cut(s) 230, 346, 516
Hpy8I GTNNAC 3 cut(s) 484, 643, 762
Hpy99I CGWCG 2 cut(s) 61, 386
HpyAV CCTTC 5 cut(s) 513, 576, 582, 769, 986
HpyCH4III ACNGT 2 cut(s) 137, 766
HpyCH4IV ACGT 2 cut(s) 8, 933
HpyCH4V TGCA 3 cut(s) 205, 586, 898
HpyF10VI GCNNNNNNNGC 8 cut(s) 21, 71, 77, 80, 447, 562, 568, 1013
HpyF3I CTNAG 3 cut(s) 20, 278, 884
HpySE526I ACGT 2 cut(s) 8, 933
Hsp92I GRCGYC 2 cut(s) 8, 933
Hsp92II CATG 2 cut(s) 403, 649
HspAI GCGC 1 cut(s) 441
Kpn2I TCCGGA 1 cut(s) 229
Kzo9I GATC 5 cut(s) 85, 213, 226, 232, 691
LmnI GCTCC 5 cut(s) 154, 269, 713, 769, 1004
Lsp1109I GCAGC 8 cut(s) 80, 83, 92, 178, 217, 374, 549, 1022
LweI GCATC 2 cut(s) 609, 885
MaeI CTAG 1 cut(s) 173
MaeII ACGT 2 cut(s) 8, 933
MaeIII GTNAC 3 cut(s) 131, 929, 934
MalI GATC 5 cut(s) 87, 215, 228, 234, 693
MbiI CCGCTC 3 cut(s) 408, 716, 1007
MboI GATC 5 cut(s) 85, 213, 226, 232, 691
MboII GAAGA 4 cut(s) 114, 425, 751, 883
MflI RGATCY 2 cut(s) 226, 691
MhlI GDGCHC 2 cut(s) 241, 306
MluCI AATT 1 cut(s) 816
MlyI GAGTC 3 cut(s) 56, 270, 532
MroI TCCGGA 1 cut(s) 229
MseI TTAA 3 cut(s) 35, 918, 1024
MslI CAYNNNNRTG 2 cut(s) 36, 254
MspA1I CMGCKG 4 cut(s) 65, 191, 562, 1016
MspI CCGG 5 cut(s) 230, 246, 254, 510, 638
MspR9I CCNGG 3 cut(s) 254, 510, 511
Mva1269I GAATGC 1 cut(s) 703
MvnI CGCG 3 cut(s) 219, 356, 446
MwoI GCNNNNNNNGC 8 cut(s) 21, 71, 77, 80, 447, 562, 568, 1013
NciI CCSGG 3 cut(s) 254, 510, 511
NdeII GATC 5 cut(s) 85, 213, 226, 232, 691
NlaIII CATG 2 cut(s) 403, 649
NlaIV GGNNCC 5 cut(s) 250, 261, 271, 304, 720
NmeAIII GCCGAG 2 cut(s) 429, 557
NmuCI GTSAC 2 cut(s) 929, 934
NspV TTCGAA 1 cut(s) 751
OliI CACNNNNGTG 1 cut(s) 254
PaqCI CACCTGC 1 cut(s) 868
PcsI WCGNNNNNNNCGW 3 cut(s) 521, 623, 680
PctI GAATGC 1 cut(s) 703
PfeI GAWTC 1 cut(s) 112
PleI GAGTC 3 cut(s) 55, 270, 532
PpsI GAGTC 3 cut(s) 55, 270, 532
PspFI CCCAGC 1 cut(s) 187
PspN4I GGNNCC 5 cut(s) 250, 261, 271, 304, 720
PspOMI GGGCCC 1 cut(s) 302
PspPI GGNCC 3 cut(s) 259, 302, 303
PsrI GAACNNNNNNTAC 2 cut(s) 745, 777
PstNI CAGNNNCTG 1 cut(s) 1016
PsuI RGATCY 2 cut(s) 226, 691
PvuII CAGCTG 2 cut(s) 191, 1016
RsaI GTAC 2 cut(s) 332, 614
RsaNI GTAC 2 cut(s) 331, 613
RseI CAYNNNNRTG 2 cut(s) 36, 254
SaqAI TTAA 3 cut(s) 35, 918, 1024
Sau3AI GATC 5 cut(s) 85, 213, 226, 232, 691
Sau96I GGNCC 3 cut(s) 259, 302, 303
ScaI AGTACT 1 cut(s) 614
SchI GAGTC 3 cut(s) 56, 270, 532
ScrFI CCNGG 3 cut(s) 254, 510, 511
SduI GDGCHC 2 cut(s) 241, 306
SfaNI GCATC 2 cut(s) 609, 885
SfcI CTRYAG 1 cut(s) 960
SfuI TTCGAA 1 cut(s) 751
SmaI CCCGGG 1 cut(s) 511
SmiMI CAYNNNNRTG 2 cut(s) 36, 254
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 1 cut(s) 816
SspMI CTAG 1 cut(s) 173
StyD4I CCNGG 3 cut(s) 252, 508, 509
TaaI ACNGT 2 cut(s) 137, 766
TaiI ACGT 2 cut(s) 11, 936
TaqI TCGA 7 cut(s) 381, 515, 524, 617, 683, 737, 751
TasI AATT 1 cut(s) 816
TatI WGTACW 1 cut(s) 612
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 3 cut(s) 35, 918, 1024
Tru9I TTAA 3 cut(s) 35, 918, 1024
TscAI CASTG 5 cut(s) 130, 151, 286, 292, 943
TseFI GTSAC 2 cut(s) 929, 934
TseI GCWGC 9 cut(s) 68, 71, 80, 191, 205, 387, 562, 1010, 1013
Tsp45I GTSAC 2 cut(s) 929, 934
TspDTI ATGAA 1 cut(s) 388
TspGWI ACGGA 2 cut(s) 282, 689
TspMI CCCGGG 1 cut(s) 509
TspRI CASTG 5 cut(s) 130, 151, 286, 292, 943
XapI RAATTY 1 cut(s) 816
XmaI CCCGGG 1 cut(s) 509
XspI CTAG 1 cut(s) 173
ZraI GACGTC 2 cut(s) 9, 934
ZrmI AGTACT 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.