AT2G20130

stem vascular tissue pattern formation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008495
Physical Location & Seq
Reverse (-)
8690293 .. 8692490
2198 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G20130.1

Sequence Viewer

Length: 771 bp
ATGGCCAATCGAGAAAGAGATCGAGAGCTTCTGATTCCCGTCGCTGATTTCGGCGACAAAGACGATGGTTCTTCTTCGAAGCCTTCTTCTTCTTCCTCTGCTTCTTCTTCGCATCAATCCGGCCACGAGACTTTAAGCTTGTTCATTAGGGGTTGGGCGTCAAAGAAGTTCATGACAGGCTGTGTTATCCTACTTCCTATTGCAGTTACTTTCTATACAACATGGTGGTTTATTCACTTTGTCGATGGATTCTTCTCTCCAATATATGCACTACTTGGAATCAACATATTTGGTTTCGGTTTTTTGACTTCCATTGCATTCATCTTCTTGGTTGGGGTGTTCATGTCTTCGTGGTTGGGGGCATCGGTTCTGAACTTGGGAGAGTGGTTTATCAAGCGGATGCCATTTGTTCGTCACATCTACAACGCCTCCAAGCAAATCAGCACTGCCATATCGCCTGATCAAAATACACAAGCTTTCAAGGAAGTTGCAATCATCAGGCATCCCCGTGTAGGTGAATATGCATTTGGCTTCATTACATCAACTGTTGTTCTCCAGAACTACCCAACCGAGGAGGAACTTTGCTGTGTATACGTTCCCACGAACCACCTTTACATTGGAGATATACTACTTGTCAACAGTAATGACGTTATCAGGCCAAACCTCTCTGTCCGGGAAGGAATTGAGATTGTTGTTTCCGGGGGAATGTCAATGCCGCAGATTCTATCGACTCTTGATAAACCTTTGGCCTCGATTGGAAATGAATCTTAA

Protein Analysis

256

Amino Acids

28.38

Weight (kDa)

5.85

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 103 - 203 3.7e-28 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 591
AciI CCGC 2 cut(s) 397, 716
AcoI YGGCCR 2 cut(s) 3, 121
AcyI GRCGYC 1 cut(s) 158
AfiI CCNNNNNNNGG 2 cut(s) 512, 571
AgsI TTSAA 1 cut(s) 481
AjuI GAANNNNNNNTTGG 2 cut(s) 510, 542
AluBI AGCT 3 cut(s) 28, 138, 476
AluI AGCT 3 cut(s) 28, 138, 476
Alw26I GTCTC 1 cut(s) 122
AoxI GGCC 4 cut(s) 3, 121, 656, 747
AsuC2I CCSGG 2 cut(s) 674, 700
AsuHPI GGTGA 1 cut(s) 527
AsuII TTCGAA 1 cut(s) 77
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 339
BccI CCATC 2 cut(s) 59, 239
BclI TGATCA 1 cut(s) 460
BcnI CCSGG 2 cut(s) 674, 700
BcoDI GTCTC 1 cut(s) 122
BisI GCNGC 1 cut(s) 716
BlsI GCNGC 1 cut(s) 717
Bme1390I CCNGG 2 cut(s) 674, 700
BmrFI CCNGG 2 cut(s) 674, 700
BmsI GCATC 4 cut(s) 121, 371, 390, 511
BpiI GAAGAC 1 cut(s) 339
BpmI CTGGAG 1 cut(s) 539
Bpu14I TTCGAA 1 cut(s) 77
BpuMI CCSGG 2 cut(s) 674, 700
BsaHI GRCGYC 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 570, 699
BsaXI ACNNNNNCTCC 2 cut(s) 413, 443
Bsc4I CCNNNNNNNGG 2 cut(s) 512, 571
Bse3DI GCAATG 1 cut(s) 312
BseDI CCNNGG 2 cut(s) 570, 699
BseGI GGATG 2 cut(s) 405, 502
BseLI CCNNNNNNNGG 2 cut(s) 512, 571
BseMI GCAATG 1 cut(s) 312
BseRI GAGGAG 1 cut(s) 587
BshFI GGCC 4 cut(s) 5, 123, 658, 749
BsiSI CCGG 3 cut(s) 120, 673, 699
BslI CCNNNNNNNGG 2 cut(s) 512, 571
BsmAI GTCTC 1 cut(s) 122
BsmI GAATGC 1 cut(s) 317
BsnI GGCC 4 cut(s) 5, 123, 658, 749
Bsp119I TTCGAA 1 cut(s) 77
Bsp143I GATC 2 cut(s) 19, 460
BspACI CCGC 2 cut(s) 397, 716
BspANI GGCC 4 cut(s) 5, 123, 658, 749
BspHI TCATGA 1 cut(s) 171
BspT104I TTCGAA 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 312
BssECI CCNNGG 2 cut(s) 570, 699
BssMI GATC 2 cut(s) 19, 460
BssNAI GTATAC 1 cut(s) 592
BssNI GRCGYC 1 cut(s) 158
BssSI CACGAG 1 cut(s) 125
Bst1107I GTATAC 1 cut(s) 592
Bst2BI CACGAG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 547, 641
BstACI GRCGYC 1 cut(s) 158
BstBI TTCGAA 1 cut(s) 77
BstF5I GGATG 2 cut(s) 405, 502
BstKTI GATC 2 cut(s) 22, 463
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 2 cut(s) 19, 460
BstSCI CCNGG 2 cut(s) 672, 698
BstV2I GAAGAC 1 cut(s) 339
BstZ17I GTATAC 1 cut(s) 592
BsuRI GGCC 4 cut(s) 5, 123, 658, 749
BtsCI GGATG 2 cut(s) 405, 502
BtsI GCAGTG 1 cut(s) 444
BtsIMutI CAGTG 1 cut(s) 444
CciI TCATGA 1 cut(s) 171
CseI GACGC 1 cut(s) 147
CviAII CATG 3 cut(s) 172, 222, 343
DpnI GATC 2 cut(s) 21, 462
DpnII GATC 2 cut(s) 19, 460
EaeI YGGCCR 2 cut(s) 3, 121
EcoT22I ATGCAT 1 cut(s) 526
FaeI CATG 3 cut(s) 175, 225, 346
FatI CATG 3 cut(s) 171, 221, 342
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 1 cut(s) 591
Fnu4HI GCNGC 1 cut(s) 716
FokI GGATG 2 cut(s) 412, 489
Fsp4HI GCNGC 1 cut(s) 716
GluI GCNGC 1 cut(s) 716
GsuI CTGGAG 1 cut(s) 539
HaeIII GGCC 4 cut(s) 5, 123, 658, 749
HapII CCGG 3 cut(s) 120, 673, 699
HgaI GACGC 1 cut(s) 147
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 3 cut(s) 175, 225, 346
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HindIII AAGCTT 2 cut(s) 136, 474
HinfI GANTC 6 cut(s) 34, 249, 279, 721, 730, 764
HpaII CCGG 3 cut(s) 120, 673, 699
HphI GGTGA 1 cut(s) 527
Hpy166II GTNNAC 2 cut(s) 592, 637
Hpy188I TCNGA 2 cut(s) 33, 372
Hpy188III TCNNGA 5 cut(s) 11, 23, 172, 556, 734
Hpy8I GTNNAC 2 cut(s) 592, 637
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 2 cut(s) 93, 671
HpyCH4III ACNGT 2 cut(s) 547, 641
HpyCH4IV ACGT 2 cut(s) 594, 648
HpyCH4V TGCA 5 cut(s) 203, 269, 317, 491, 524
HpySE526I ACGT 2 cut(s) 594, 648
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 3 cut(s) 175, 225, 346
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 2 cut(s) 19, 460
LpnPI CCDG 8 cut(s) 133, 162, 471, 484, 569, 640, 686, 712
LweI GCATC 4 cut(s) 121, 371, 390, 511
MaeII ACGT 2 cut(s) 594, 648
MaeIII GTNAC 2 cut(s) 205, 413
MalI GATC 2 cut(s) 21, 462
MboI GATC 2 cut(s) 19, 460
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 681
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 724
MnlI CCTC 6 cut(s) 106, 439, 565, 568, 674, 760
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 526
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 134, 769
MslI CAYNNNNRTG 1 cut(s) 507
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 120, 673, 699
MspR9I CCNGG 2 cut(s) 674, 700
Mva1269I GAATGC 1 cut(s) 317
NciI CCSGG 2 cut(s) 674, 700
NdeII GATC 2 cut(s) 19, 460
NlaIII CATG 3 cut(s) 175, 225, 346
NmuCI GTSAC 1 cut(s) 413
NsiI ATGCAT 1 cut(s) 526
NspV TTCGAA 1 cut(s) 77
PagI TCATGA 1 cut(s) 171
PctI GAATGC 1 cut(s) 317
PfeI GAWTC 5 cut(s) 34, 249, 279, 721, 764
PfoI TCCNGGA 1 cut(s) 672
PkrI GCNGC 1 cut(s) 717
PleI GAGTC 1 cut(s) 724
PpsI GAGTC 1 cut(s) 724
PsrI GAACNNNNNNTAC 2 cut(s) 596, 628
RseI CAYNNNNRTG 1 cut(s) 507
SaqAI TTAA 2 cut(s) 134, 769
SatI GCNGC 1 cut(s) 716
Sau3AI GATC 2 cut(s) 19, 460
SchI GAGTC 1 cut(s) 724
ScrFI CCNGG 2 cut(s) 674, 700
SetI ASST 9 cut(s) 30, 140, 478, 517, 597, 612, 651, 666, 745
SfaNI GCATC 4 cut(s) 121, 371, 390, 511
SfuI TTCGAA 1 cut(s) 77
SmiMI CAYNNNNRTG 1 cut(s) 507
Sse9I AATT 1 cut(s) 681
SsiI CCGC 2 cut(s) 397, 716
StyD4I CCNGG 2 cut(s) 672, 698
TaaI ACNGT 2 cut(s) 547, 641
TaiI ACGT 2 cut(s) 597, 651
TaqI TCGA 6 cut(s) 10, 22, 77, 243, 728, 752
TasI AATT 1 cut(s) 681
TauI GCSGC 1 cut(s) 718
TfiI GAWTC 5 cut(s) 34, 249, 279, 721, 764
Tru1I TTAA 2 cut(s) 134, 769
Tru9I TTAA 2 cut(s) 134, 769
TscAI CASTG 1 cut(s) 451
TseFI GTSAC 1 cut(s) 413
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 5 cut(s) 133, 160, 310, 331, 523
TspRI CASTG 1 cut(s) 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 614
XmiI GTMKAC 1 cut(s) 591
Zsp2I ATGCAT 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.