Rw2G004570

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008495
Physical Location & Seq
Reverse (-)
4326069 .. 4329289
3221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G004570.1

Sequence Viewer

Length: 807 bp
ATGGGCGATGAGAAGTCATCGTCGTCGTCGTCAATTGTAATGGCAAGCAGTAGAGACAGAGAGCCGCGAGATCGAGAGCTTCTCATTCCGGTCGCCGACTCCGGCGATGAAGACCTTTCTTCCAAGCCCTCTTCGTCTTCGTCCTCCTCGCACCACCTCACTGGCCGCGAGACTTTTTACAAAGTTTTTAGAAGTTGGGCGTCAAAGAAGTTCATGACTGGATGTGTGATCCTATTTCCAATAGCCATCACTTTCTACATAACTTGGTGGTTTATTCACTTTGTGGATGGCTTCTTCTCTCCAATATATGCTCAGCTAGGAATCAACATTTTCGGTCTTGGATTTGTAACTTCCATTACGTTCATATTTTTGGTTGGGGTGTTCATGTCATCATGGCTGGGTGCATCTGTCCTGGGTCTCGGTGAGTGGTTTATCAAAAGAATGCCATTCGTTCGTCATATCTACAATGCCTCCAAGCAGATTAGTTCTGCCATATCCCCAGATCAAAACACGCAAGCCTTCAAGGAAGTGGCCATCATAAGGCATCCACGTATTGGAGAATATGCATTTGGGTTCATTACTTCATCAGTTACCCTTCAGAACTATGCTGGTGATGAGGAATTGTGCTGTGTTTATGTTCCCACGAACCATTTGTATATTGGTGATGTCTTTCTGGTCAGCACCAAGGATGTGATCAGGCCAAATTTATCCGTACGGGAAGGAATTGAAATTGTTGTGTCTGGAGGCATGTCGATGCCCCAGATCCTGTCAACGGTTGATTCCCGGATTATGCCAGTGGTTAGCTGA

Protein Analysis

268

Amino Acids

29.82

Weight (kDa)

6.3

Isoelectric Point (pI)

53.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 117 - 217 1.7e-27 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 554
AccII CGCG 2 cut(s) 67, 168
AciI CCGC 2 cut(s) 65, 166
AclWI GGATC 2 cut(s) 223, 757
AcoI YGGCCR 2 cut(s) 163, 531
AcsI RAATTY 1 cut(s) 703
AcuI CTGAAG 1 cut(s) 581
AcyI GRCGYC 1 cut(s) 200
AdeI CACNNNGTG 1 cut(s) 283
AfaI GTAC 1 cut(s) 714
AfiI CCNNNNNNNGG 3 cut(s) 540, 554, 772
AgsI TTSAA 2 cut(s) 523, 728
AjnI CCWGG 1 cut(s) 411
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AluBI AGCT 3 cut(s) 79, 316, 804
AluI AGCT 3 cut(s) 79, 316, 804
Alw26I GTCTC 3 cut(s) 48, 164, 422
AlwI GGATC 2 cut(s) 223, 757
AlwNI CAGNNNCTG 1 cut(s) 766
AoxI GGCC 3 cut(s) 163, 531, 698
ApoI RAATTY 1 cut(s) 703
AsuC2I CCSGG 1 cut(s) 784
AsuHPI GGTGA 3 cut(s) 434, 623, 674
BalI TGGCCA 1 cut(s) 533
BbsI GAAGAC 2 cut(s) 117, 129
BccI CCATC 3 cut(s) 254, 281, 542
BciT130I CCWGG 1 cut(s) 413
BclI TGATCA 1 cut(s) 693
BcnI CCSGG 1 cut(s) 784
BcoDI GTCTC 3 cut(s) 48, 164, 422
BfaI CTAG 1 cut(s) 317
BisI GCNGC 2 cut(s) 65, 166
BlpI GCTNAGC 1 cut(s) 312
BlsI GCNGC 2 cut(s) 66, 167
Bme1390I CCNGG 2 cut(s) 413, 784
BmrFI CCNGG 2 cut(s) 413, 784
BmsI GCATC 3 cut(s) 413, 553, 744
BpiI GAAGAC 2 cut(s) 117, 129
BplI GAGNNNNNCTC 2 cut(s) 66, 98
BpmI CTGGAG 1 cut(s) 762
Bpu1102I GCTNAGC 1 cut(s) 312
BpuMI CCSGG 1 cut(s) 784
BsaAI YACGTR 1 cut(s) 551
BsaHI GRCGYC 1 cut(s) 200
BsaI GGTCTC 1 cut(s) 422
BsaJI CCNNGG 2 cut(s) 412, 684
BsaWI WCCGGW 1 cut(s) 88
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bsc4I CCNNNNNNNGG 3 cut(s) 540, 554, 772
Bse1I ACTGG 3 cut(s) 166, 223, 794
BseBI CCWGG 1 cut(s) 413
BseDI CCNNGG 2 cut(s) 412, 684
BseGI GGATG 4 cut(s) 227, 292, 544, 694
BseLI CCNNNNNNNGG 3 cut(s) 540, 554, 772
BseMII CTCAG 1 cut(s) 326
BseNI ACTGG 3 cut(s) 166, 223, 794
BseRI GAGGAG 1 cut(s) 136
BseYI CCCAGC 1 cut(s) 397
Bsh1236I CGCG 2 cut(s) 67, 168
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 3 cut(s) 165, 533, 700
BsiEI CGRYCG 1 cut(s) 93
BsiSI CCGG 3 cut(s) 89, 102, 784
BsiWI CGTACG 1 cut(s) 712
BslI CCNNNNNNNGG 3 cut(s) 540, 554, 772
BsmAI GTCTC 3 cut(s) 48, 164, 422
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 3 cut(s) 165, 533, 700
Bso31I GGTCTC 1 cut(s) 422
Bsp143I GATC 5 cut(s) 70, 228, 502, 693, 762
Bsp1720I GCTNAGC 1 cut(s) 312
BspACI CCGC 2 cut(s) 65, 166
BspANI GGCC 3 cut(s) 165, 533, 700
BspCNI CTCAG 1 cut(s) 325
BspFNI CGCG 2 cut(s) 67, 168
BspHI TCATGA 1 cut(s) 213
BspPI GGATC 2 cut(s) 223, 757
BspTNI GGTCTC 1 cut(s) 422
BsrI ACTGG 3 cut(s) 166, 223, 794
BssECI CCNNGG 2 cut(s) 412, 684
BssMI GATC 5 cut(s) 70, 228, 502, 693, 762
BssNI GRCGYC 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 684
Bst2UI CCWGG 1 cut(s) 413
Bst4CI ACNGT 1 cut(s) 775
Bst6I CTCTTC 1 cut(s) 136
BstACI GRCGYC 1 cut(s) 200
BstBAI YACGTR 1 cut(s) 551
BstC8I GCNNGC 2 cut(s) 46, 516
BstDEI CTNAG 1 cut(s) 312
BstF5I GGATG 4 cut(s) 227, 292, 544, 694
BstFNI CGCG 2 cut(s) 67, 168
BstKTI GATC 5 cut(s) 73, 231, 505, 696, 765
BstMAI GTCTC 3 cut(s) 48, 164, 422
BstMBI GATC 5 cut(s) 70, 228, 502, 693, 762
BstMCI CGRYCG 1 cut(s) 93
BstNI CCWGG 1 cut(s) 413
BstNSI RCATGY 1 cut(s) 751
BstSCI CCNGG 2 cut(s) 411, 782
BstUI CGCG 2 cut(s) 67, 168
BstV2I GAAGAC 2 cut(s) 117, 129
BstX2I RGATCY 1 cut(s) 762
BstXI CCANNNNNNTGG 1 cut(s) 161
BstYI RGATCY 1 cut(s) 762
BsuRI GGCC 3 cut(s) 165, 533, 700
BtgZI GCGATG 2 cut(s) 21, 120
BtsCI GGATG 4 cut(s) 227, 292, 544, 694
BtsIMutI CAGTG 2 cut(s) 159, 801
Cac8I GCNNGC 2 cut(s) 46, 516
CaiI CAGNNNCTG 1 cut(s) 766
CciI TCATGA 1 cut(s) 213
CseI GACGC 1 cut(s) 189
Csp6I GTAC 1 cut(s) 713
CviAII CATG 4 cut(s) 214, 385, 393, 748
CviQI GTAC 1 cut(s) 713
DdeI CTNAG 1 cut(s) 312
DpnI GATC 5 cut(s) 72, 230, 504, 695, 764
DpnII GATC 5 cut(s) 70, 228, 502, 693, 762
DraIII CACNNNGTG 1 cut(s) 283
EaeI YGGCCR 2 cut(s) 163, 531
Eam1104I CTCTTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 684
Eco31I GGTCTC 1 cut(s) 422
Eco57I CTGAAG 1 cut(s) 581
EcoRII CCWGG 1 cut(s) 411
EcoT14I CCWWGG 1 cut(s) 684
EcoT22I ATGCAT 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 684
FaeI CATG 4 cut(s) 217, 388, 396, 751
FatI CATG 4 cut(s) 213, 384, 392, 747
FbaI TGATCA 1 cut(s) 693
Fnu4HI GCNGC 2 cut(s) 65, 166
FokI GGATG 4 cut(s) 234, 299, 531, 701
Fsp4HI GCNGC 2 cut(s) 65, 166
FspBI CTAG 1 cut(s) 317
GluI GCNGC 2 cut(s) 65, 166
GsaI CCCAGC 1 cut(s) 401
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 3 cut(s) 165, 533, 700
HapII CCGG 3 cut(s) 89, 102, 784
HgaI GACGC 1 cut(s) 189
Hin1I GRCGYC 1 cut(s) 200
Hin1II CATG 4 cut(s) 217, 388, 396, 751
HincII GTYRAC 1 cut(s) 771
HindII GTYRAC 1 cut(s) 771
HinfI GANTC 3 cut(s) 98, 321, 779
HpaII CCGG 3 cut(s) 89, 102, 784
HphI GGTGA 3 cut(s) 434, 623, 674
Hpy166II GTNNAC 1 cut(s) 771
Hpy188I TCNGA 1 cut(s) 600
Hpy188III TCNNGA 3 cut(s) 74, 214, 741
Hpy8I GTNNAC 1 cut(s) 771
Hpy99I CGWCG 3 cut(s) 25, 28, 31
HpyAV CCTTC 3 cut(s) 529, 605, 713
HpyCH4III ACNGT 1 cut(s) 775
HpyCH4IV ACGT 2 cut(s) 359, 550
HpyCH4V TGCA 2 cut(s) 404, 566
HpyF3I CTNAG 1 cut(s) 312
HpySE526I ACGT 2 cut(s) 359, 550
Hsp92I GRCGYC 1 cut(s) 200
Hsp92II CATG 4 cut(s) 217, 388, 396, 751
Ksp22I TGATCA 1 cut(s) 693
Kzo9I GATC 5 cut(s) 70, 228, 502, 693, 762
LweI GCATC 3 cut(s) 413, 553, 744
MaeI CTAG 1 cut(s) 317
MaeII ACGT 2 cut(s) 359, 550
MaeIII GTNAC 2 cut(s) 346, 589
MalI GATC 5 cut(s) 72, 230, 504, 695, 764
MboI GATC 5 cut(s) 70, 228, 502, 693, 762
MboII GAAGA 5 cut(s) 111, 122, 123, 129, 286
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 1 cut(s) 762
MlsI TGGCCA 1 cut(s) 533
MluCI AATT 5 cut(s) 33, 620, 703, 723, 729
MluNI TGGCCA 1 cut(s) 533
MlyI GAGTC 1 cut(s) 92
MnlI CCTC 7 cut(s) 139, 154, 157, 167, 481, 610, 737
Mox20I TGGCCA 1 cut(s) 533
Mph1103I ATGCAT 1 cut(s) 568
MscI TGGCCA 1 cut(s) 533
MslI CAYNNNNRTG 1 cut(s) 752
Msp20I TGGCCA 1 cut(s) 533
MspI CCGG 3 cut(s) 89, 102, 784
MspR9I CCNGG 2 cut(s) 413, 784
MunI CAATTG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 447
MvaI CCWGG 1 cut(s) 413
MvnI CGCG 2 cut(s) 67, 168
NciI CCSGG 1 cut(s) 784
NdeII GATC 5 cut(s) 70, 228, 502, 693, 762
NlaIII CATG 4 cut(s) 217, 388, 396, 751
NsiI ATGCAT 1 cut(s) 568
NspI RCATGY 1 cut(s) 751
PagI TCATGA 1 cut(s) 213
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PctI GAATGC 1 cut(s) 447
PfeI GAWTC 2 cut(s) 321, 779
Pfl23II CGTACG 1 cut(s) 712
PflMI CCANNNNNTGG 1 cut(s) 554
PfoI TCCNGGA 1 cut(s) 782
PkrI GCNGC 2 cut(s) 66, 167
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Ppu21I YACGTR 1 cut(s) 551
Psp6I CCWGG 1 cut(s) 411
PspFI CCCAGC 1 cut(s) 397
PspGI CCWGG 1 cut(s) 411
PspLI CGTACG 1 cut(s) 712
PstNI CAGNNNCTG 1 cut(s) 766
PsuI RGATCY 1 cut(s) 762
RsaI GTAC 1 cut(s) 714
RsaNI GTAC 1 cut(s) 713
RseI CAYNNNNRTG 1 cut(s) 752
SatI GCNGC 2 cut(s) 65, 166
Sau3AI GATC 5 cut(s) 70, 228, 502, 693, 762
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 2 cut(s) 413, 784
SetI ASST 7 cut(s) 81, 117, 159, 318, 362, 553, 806
SfaNI GCATC 3 cut(s) 413, 553, 744
SmiMI CAYNNNNRTG 1 cut(s) 752
Sse9I AATT 5 cut(s) 33, 620, 703, 723, 729
SsiI CCGC 2 cut(s) 65, 166
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 2 cut(s) 411, 782
StyI CCWWGG 1 cut(s) 684
TaaI ACNGT 1 cut(s) 775
TaiI ACGT 2 cut(s) 362, 553
TaqI TCGA 2 cut(s) 73, 752
TaqII GACCGA 1 cut(s) 323
TasI AATT 5 cut(s) 33, 620, 703, 723, 729
TauI GCSGC 2 cut(s) 67, 168
TfiI GAWTC 2 cut(s) 321, 779
TscAI CASTG 2 cut(s) 166, 801
TspDTI ATGAA 6 cut(s) 123, 202, 352, 373, 565, 573
TspGWI ACGGA 1 cut(s) 700
TspRI CASTG 2 cut(s) 166, 801
Van91I CCANNNNNTGG 1 cut(s) 554
XapI RAATTY 1 cut(s) 703
XceI RCATGY 1 cut(s) 751
XcmI CCANNNNNNNNNTGG 1 cut(s) 656
XspI CTAG 1 cut(s) 317
Zsp2I ATGCAT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.