Rh2AG051000

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008495
Physical Location & Seq
Forward (+)
4033236 .. 4036932
3697 bp
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UTR
Exon/CDS
Intron
Rh2AG051000.1

Sequence Viewer

Length: 819 bp
ATGGGCGATGAGAAATCATCGTCGTCGTCGTCGTCGTCGTCGTCAATTGTAATGGCAAGCAGTAGAGACAGAGAGCCGCGAGATCGAGAGCTTCTCATTCCGGTCGCCGACTCCGGCGATGAAGACCTTTCTTCCAAGCCCTCTTCGTCTTCGTCCTCCTCGCACCACCTCACTGGCCGCGAGACTTTTTACAAAGTTTTTAGAAGTTGGGCGTCAAAGAAGTTCATGACTGGATGCGTGATCCTATTTCCAATAGCCATCACTTTCTACATAACTTGGTGGTTTATTCACTTTGTGGATGGCTTCTTCTCTCCAATATATGCTCAGCTAGGAATCAACATTTTCGGTCTTGGATTTGTAACTTCCATTACGTTCATATTTTTGGTTGGGGTGTTCATGTCATCATGGCTGGGTGCATCTGTCCTGGGTCTCGGTGAGTGGTTTATCAAAAGAATGCCATTCGTTCGTCATATCTACAATGCCTCCAAGCAGATTAGTTCTGCCATATCCCCAGATCAAAACACGCAAGCCTTCAAGGAAGTGGCCATCATAAGGCATCCACGTATTGGAGAATATGCATTTGGGTTCATTACTTCATCAGTTACCCTTCAGAACTATGCTGGTGATGAGGAATTGTGCTGTGTTTATGTTCCCACGAACCATTTGTATATTGGTGATGTCTTTCTGGTCAGCACCAAGGATGTGATCAGGCCAAATTTATCCGTACGGGAAGGAATTGAAATTGTTGTGTCTGGAGGCATGTCGATGCCCCAGATCCTGTCAACGGTTGATTCCCGGATTATGCCAGTGGTTAGCTGA

Protein Analysis

272

Amino Acids

30.17

Weight (kDa)

6.3

Isoelectric Point (pI)

55.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 121 - 221 1.7e-27 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 566
AccII CGCG 2 cut(s) 79, 180
AciI CCGC 2 cut(s) 77, 178
AclWI GGATC 2 cut(s) 235, 769
AcoI YGGCCR 2 cut(s) 175, 543
AcsI RAATTY 1 cut(s) 715
AcuI CTGAAG 1 cut(s) 593
AcyI GRCGYC 1 cut(s) 212
AdeI CACNNNGTG 1 cut(s) 295
AfaI GTAC 1 cut(s) 726
AfiI CCNNNNNNNGG 3 cut(s) 552, 566, 784
AgsI TTSAA 2 cut(s) 535, 740
AjnI CCWGG 1 cut(s) 423
AjuI GAANNNNNNNTTGG 2 cut(s) 564, 596
AluBI AGCT 3 cut(s) 91, 328, 816
AluI AGCT 3 cut(s) 91, 328, 816
Alw26I GTCTC 3 cut(s) 60, 176, 434
AlwI GGATC 2 cut(s) 235, 769
AlwNI CAGNNNCTG 1 cut(s) 778
AoxI GGCC 3 cut(s) 175, 543, 710
ApoI RAATTY 1 cut(s) 715
AsuC2I CCSGG 1 cut(s) 796
AsuHPI GGTGA 3 cut(s) 446, 635, 686
BalI TGGCCA 1 cut(s) 545
BbsI GAAGAC 2 cut(s) 129, 141
BccI CCATC 3 cut(s) 266, 293, 554
BciT130I CCWGG 1 cut(s) 425
BclI TGATCA 1 cut(s) 705
BcnI CCSGG 1 cut(s) 796
BcoDI GTCTC 3 cut(s) 60, 176, 434
BfaI CTAG 1 cut(s) 329
BisI GCNGC 2 cut(s) 77, 178
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 2 cut(s) 78, 179
Bme1390I CCNGG 2 cut(s) 425, 796
BmrFI CCNGG 2 cut(s) 425, 796
BmsI GCATC 4 cut(s) 224, 425, 565, 756
BpiI GAAGAC 2 cut(s) 129, 141
BplI GAGNNNNNCTC 2 cut(s) 78, 110
BpmI CTGGAG 1 cut(s) 774
Bpu1102I GCTNAGC 1 cut(s) 324
BpuMI CCSGG 1 cut(s) 796
BsaAI YACGTR 1 cut(s) 563
BsaHI GRCGYC 1 cut(s) 212
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 2 cut(s) 424, 696
BsaWI WCCGGW 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 552, 566, 784
Bse1I ACTGG 3 cut(s) 178, 235, 806
BseBI CCWGG 1 cut(s) 425
BseDI CCNNGG 2 cut(s) 424, 696
BseGI GGATG 4 cut(s) 239, 304, 556, 706
BseLI CCNNNNNNNGG 3 cut(s) 552, 566, 784
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 3 cut(s) 178, 235, 806
BseRI GAGGAG 1 cut(s) 148
BseYI CCCAGC 1 cut(s) 409
Bsh1236I CGCG 2 cut(s) 79, 180
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 3 cut(s) 177, 545, 712
BsiEI CGRYCG 1 cut(s) 105
BsiSI CCGG 3 cut(s) 101, 114, 796
BsiWI CGTACG 1 cut(s) 724
BslI CCNNNNNNNGG 3 cut(s) 552, 566, 784
BsmAI GTCTC 3 cut(s) 60, 176, 434
BsmI GAATGC 1 cut(s) 459
BsnI GGCC 3 cut(s) 177, 545, 712
Bso31I GGTCTC 1 cut(s) 434
Bsp143I GATC 5 cut(s) 82, 240, 514, 705, 774
Bsp1720I GCTNAGC 1 cut(s) 324
BspACI CCGC 2 cut(s) 77, 178
BspANI GGCC 3 cut(s) 177, 545, 712
BspCNI CTCAG 1 cut(s) 337
BspFNI CGCG 2 cut(s) 79, 180
BspHI TCATGA 1 cut(s) 225
BspPI GGATC 2 cut(s) 235, 769
BspTNI GGTCTC 1 cut(s) 434
BsrI ACTGG 3 cut(s) 178, 235, 806
BssECI CCNNGG 2 cut(s) 424, 696
BssMI GATC 5 cut(s) 82, 240, 514, 705, 774
BssNI GRCGYC 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 696
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 1 cut(s) 787
Bst6I CTCTTC 1 cut(s) 148
BstACI GRCGYC 1 cut(s) 212
BstBAI YACGTR 1 cut(s) 563
BstC8I GCNNGC 2 cut(s) 58, 528
BstDEI CTNAG 1 cut(s) 324
BstF5I GGATG 4 cut(s) 239, 304, 556, 706
BstFNI CGCG 2 cut(s) 79, 180
BstKTI GATC 5 cut(s) 85, 243, 517, 708, 777
BstMAI GTCTC 3 cut(s) 60, 176, 434
BstMBI GATC 5 cut(s) 82, 240, 514, 705, 774
BstMCI CGRYCG 1 cut(s) 105
BstNI CCWGG 1 cut(s) 425
BstNSI RCATGY 1 cut(s) 763
BstSCI CCNGG 2 cut(s) 423, 794
BstUI CGCG 2 cut(s) 79, 180
BstV2I GAAGAC 2 cut(s) 129, 141
BstX2I RGATCY 1 cut(s) 774
BstXI CCANNNNNNTGG 1 cut(s) 173
BstYI RGATCY 1 cut(s) 774
BsuRI GGCC 3 cut(s) 177, 545, 712
BtgZI GCGATG 2 cut(s) 21, 132
BtsCI GGATG 4 cut(s) 239, 304, 556, 706
BtsIMutI CAGTG 2 cut(s) 171, 813
Cac8I GCNNGC 2 cut(s) 58, 528
CaiI CAGNNNCTG 1 cut(s) 778
CciI TCATGA 1 cut(s) 225
CseI GACGC 1 cut(s) 201
Csp6I GTAC 1 cut(s) 725
CviAII CATG 4 cut(s) 226, 397, 405, 760
CviQI GTAC 1 cut(s) 725
DdeI CTNAG 1 cut(s) 324
DpnI GATC 5 cut(s) 84, 242, 516, 707, 776
DpnII GATC 5 cut(s) 82, 240, 514, 705, 774
DraIII CACNNNGTG 1 cut(s) 295
EaeI YGGCCR 2 cut(s) 175, 543
Eam1104I CTCTTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 696
Eco31I GGTCTC 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 593
EcoRII CCWGG 1 cut(s) 423
EcoT14I CCWWGG 1 cut(s) 696
EcoT22I ATGCAT 1 cut(s) 580
ErhI CCWWGG 1 cut(s) 696
FaeI CATG 4 cut(s) 229, 400, 408, 763
FatI CATG 4 cut(s) 225, 396, 404, 759
FbaI TGATCA 1 cut(s) 705
Fnu4HI GCNGC 2 cut(s) 77, 178
FokI GGATG 4 cut(s) 246, 311, 543, 713
Fsp4HI GCNGC 2 cut(s) 77, 178
FspBI CTAG 1 cut(s) 329
GluI GCNGC 2 cut(s) 77, 178
GsaI CCCAGC 1 cut(s) 413
GsuI CTGGAG 1 cut(s) 774
HaeIII GGCC 3 cut(s) 177, 545, 712
HapII CCGG 3 cut(s) 101, 114, 796
HgaI GACGC 1 cut(s) 201
Hin1I GRCGYC 1 cut(s) 212
Hin1II CATG 4 cut(s) 229, 400, 408, 763
HincII GTYRAC 1 cut(s) 783
HindII GTYRAC 1 cut(s) 783
HinfI GANTC 3 cut(s) 110, 333, 791
HpaII CCGG 3 cut(s) 101, 114, 796
HphI GGTGA 3 cut(s) 446, 635, 686
Hpy166II GTNNAC 1 cut(s) 783
Hpy188I TCNGA 1 cut(s) 612
Hpy188III TCNNGA 3 cut(s) 86, 226, 753
Hpy8I GTNNAC 1 cut(s) 783
Hpy99I CGWCG 7 cut(s) 25, 28, 31, 34, 37, 40, 43
HpyAV CCTTC 3 cut(s) 541, 617, 725
HpyCH4III ACNGT 1 cut(s) 787
HpyCH4IV ACGT 2 cut(s) 371, 562
HpyCH4V TGCA 2 cut(s) 416, 578
HpyF3I CTNAG 1 cut(s) 324
HpySE526I ACGT 2 cut(s) 371, 562
Hsp92I GRCGYC 1 cut(s) 212
Hsp92II CATG 4 cut(s) 229, 400, 408, 763
Ksp22I TGATCA 1 cut(s) 705
Kzo9I GATC 5 cut(s) 82, 240, 514, 705, 774
LweI GCATC 4 cut(s) 224, 425, 565, 756
MaeI CTAG 1 cut(s) 329
MaeII ACGT 2 cut(s) 371, 562
MaeIII GTNAC 2 cut(s) 358, 601
MalI GATC 5 cut(s) 84, 242, 516, 707, 776
MboI GATC 5 cut(s) 82, 240, 514, 705, 774
MboII GAAGA 5 cut(s) 123, 134, 135, 141, 298
MfeI CAATTG 1 cut(s) 45
MflI RGATCY 1 cut(s) 774
MlsI TGGCCA 1 cut(s) 545
MluCI AATT 5 cut(s) 45, 632, 715, 735, 741
MluNI TGGCCA 1 cut(s) 545
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 7 cut(s) 151, 166, 169, 179, 493, 622, 749
Mox20I TGGCCA 1 cut(s) 545
Mph1103I ATGCAT 1 cut(s) 580
MscI TGGCCA 1 cut(s) 545
MslI CAYNNNNRTG 1 cut(s) 764
Msp20I TGGCCA 1 cut(s) 545
MspI CCGG 3 cut(s) 101, 114, 796
MspR9I CCNGG 2 cut(s) 425, 796
MunI CAATTG 1 cut(s) 45
Mva1269I GAATGC 1 cut(s) 459
MvaI CCWGG 1 cut(s) 425
MvnI CGCG 2 cut(s) 79, 180
NciI CCSGG 1 cut(s) 796
NdeII GATC 5 cut(s) 82, 240, 514, 705, 774
NlaIII CATG 4 cut(s) 229, 400, 408, 763
NsiI ATGCAT 1 cut(s) 580
NspI RCATGY 1 cut(s) 763
PagI TCATGA 1 cut(s) 225
PcsI WCGNNNNNNNCGW 5 cut(s) 26, 29, 32, 35, 38
PctI GAATGC 1 cut(s) 459
PfeI GAWTC 2 cut(s) 333, 791
Pfl23II CGTACG 1 cut(s) 724
PflMI CCANNNNNTGG 1 cut(s) 566
PfoI TCCNGGA 1 cut(s) 794
PkrI GCNGC 2 cut(s) 78, 179
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
Ppu21I YACGTR 1 cut(s) 563
Psp6I CCWGG 1 cut(s) 423
PspFI CCCAGC 1 cut(s) 409
PspGI CCWGG 1 cut(s) 423
PspLI CGTACG 1 cut(s) 724
PstNI CAGNNNCTG 1 cut(s) 778
PsuI RGATCY 1 cut(s) 774
RsaI GTAC 1 cut(s) 726
RsaNI GTAC 1 cut(s) 725
RseI CAYNNNNRTG 1 cut(s) 764
SatI GCNGC 2 cut(s) 77, 178
Sau3AI GATC 5 cut(s) 82, 240, 514, 705, 774
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 2 cut(s) 425, 796
SetI ASST 7 cut(s) 93, 129, 171, 330, 374, 565, 818
SfaNI GCATC 4 cut(s) 224, 425, 565, 756
SmiMI CAYNNNNRTG 1 cut(s) 764
Sse9I AATT 5 cut(s) 45, 632, 715, 735, 741
SsiI CCGC 2 cut(s) 77, 178
SspMI CTAG 1 cut(s) 329
StyD4I CCNGG 2 cut(s) 423, 794
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 1 cut(s) 787
TaiI ACGT 2 cut(s) 374, 565
TaqI TCGA 2 cut(s) 85, 764
TaqII GACCGA 1 cut(s) 335
TasI AATT 5 cut(s) 45, 632, 715, 735, 741
TauI GCSGC 2 cut(s) 79, 180
TfiI GAWTC 2 cut(s) 333, 791
TscAI CASTG 2 cut(s) 178, 813
TspDTI ATGAA 6 cut(s) 135, 214, 364, 385, 577, 585
TspGWI ACGGA 1 cut(s) 712
TspRI CASTG 2 cut(s) 178, 813
Van91I CCANNNNNTGG 1 cut(s) 566
XapI RAATTY 1 cut(s) 715
XceI RCATGY 1 cut(s) 763
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XspI CTAG 1 cut(s) 329
Zsp2I ATGCAT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.