Rroxscaffold_2G00151400

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008495
Physical Location & Seq
Reverse (-)
88652893 .. 88656675
3783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00151400.1

Sequence Viewer

Length: 804 bp
ATGGGAGATGAGAAGTCGTCGTCGTCGTCAATTGTAATGGCAAGCAGCAGAGACAGAGAGCCGCGAGATCGAGAGCTTCTCATTCCGGTCGCCGACTCCGGCGATGAAGACCTTTCTTCCAAGCCGTCTTCGTCTTCGTCCTCCTCGCACCACCTCACTGGCCGCGAGACTTTTTACAAAGTTTTTAGAAGTTGGGCGTCAAAGAAGTTCATGACTGGATGCGTGATCCTATTTCCAATAGCCATCACTTTCTACATAACGTGGTGGTTTATTCACTTTGTGGATGGCTTCTTCTCTCCAATATATGCTCAGCTAGGAATCAACATTTTCGGTCTTGGATTCGTAACTTCCATTACATTCATATTTTTGGTTGGGGTGTTCATGTCATCATGGCTGGGTGCATCTGTCCTGGGTCTTGGTGAGTGGTTTATCAAAAGAATGCCATTCGTTCGTCATATCTACAATGCCTCCAAGCAGATTAGTTCCGCCATATCCCCAGATCAAAACACGCAAGCCTTCAAGGAAGTGGCCATCATAAGGCATCCACGTATTGGAGAATATGCATTTGGGTTCATTACTTCATCAGTTACCCTTCAGAACTATGCTGGTGATGAGGAATTGTGCTGTGTTTATGTTCCCACTAACCATTTGTATATTGGTGATGTCTTTCTGGTCAGCACCAAGGATGTGATTAGGCCAAATTTATCCGTACGGGAGGGAATTGAAATTGTTGTGTCTGGAGGCATGTCAATGCCCCAGATCCTGTCAACGGTTGATTCCCGGATTATGCCAGTGGTTAGCTGA

Protein Analysis

267

Amino Acids

29.73

Weight (kDa)

6.3

Isoelectric Point (pI)

52.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 116 - 216 1.6e-27 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 551
AccII CGCG 2 cut(s) 64, 165
AciI CCGC 3 cut(s) 62, 163, 486
AclWI GGATC 2 cut(s) 220, 754
AcoI YGGCCR 2 cut(s) 160, 528
AcsI RAATTY 1 cut(s) 700
AcuI CTGAAG 1 cut(s) 578
AcyI GRCGYC 1 cut(s) 197
AdeI CACNNNGTG 1 cut(s) 280
AfaI GTAC 1 cut(s) 711
AfiI CCNNNNNNNGG 3 cut(s) 537, 551, 769
AgsI TTSAA 2 cut(s) 520, 725
AjnI CCWGG 1 cut(s) 408
AjuI GAANNNNNNNTTGG 2 cut(s) 549, 581
AluBI AGCT 3 cut(s) 76, 313, 801
AluI AGCT 3 cut(s) 76, 313, 801
Alw26I GTCTC 2 cut(s) 45, 161
AlwI GGATC 2 cut(s) 220, 754
AlwNI CAGNNNCTG 1 cut(s) 763
AoxI GGCC 3 cut(s) 160, 528, 695
ApeKI GCWGC 1 cut(s) 45
ApoI RAATTY 1 cut(s) 700
AsuC2I CCSGG 1 cut(s) 781
AsuHPI GGTGA 3 cut(s) 431, 620, 671
BalI TGGCCA 1 cut(s) 530
BbsI GAAGAC 3 cut(s) 114, 120, 126
BbvI GCAGC 1 cut(s) 57
BccI CCATC 3 cut(s) 251, 278, 539
BceAI ACGGC 1 cut(s) 109
BciT130I CCWGG 1 cut(s) 410
BcnI CCSGG 1 cut(s) 781
BcoDI GTCTC 2 cut(s) 45, 161
BfaI CTAG 1 cut(s) 314
BisI GCNGC 3 cut(s) 46, 62, 163
BlpI GCTNAGC 1 cut(s) 309
BlsI GCNGC 3 cut(s) 47, 63, 164
Bme1390I CCNGG 2 cut(s) 410, 781
BmrFI CCNGG 2 cut(s) 410, 781
BmsI GCATC 3 cut(s) 209, 410, 550
BpiI GAAGAC 3 cut(s) 114, 120, 126
BplI GAGNNNNNCTC 2 cut(s) 63, 95
BpmI CTGGAG 1 cut(s) 759
Bpu1102I GCTNAGC 1 cut(s) 309
BpuMI CCSGG 1 cut(s) 781
BsaAI YACGTR 1 cut(s) 548
BsaHI GRCGYC 1 cut(s) 197
BsaJI CCNNGG 2 cut(s) 409, 681
BsaWI WCCGGW 1 cut(s) 85
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 3 cut(s) 537, 551, 769
Bse1I ACTGG 3 cut(s) 163, 220, 791
BseBI CCWGG 1 cut(s) 410
BseDI CCNNGG 2 cut(s) 409, 681
BseGI GGATG 4 cut(s) 224, 289, 541, 691
BseLI CCNNNNNNNGG 3 cut(s) 537, 551, 769
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 3 cut(s) 163, 220, 791
BseRI GAGGAG 1 cut(s) 133
BseXI GCAGC 1 cut(s) 57
BseYI CCCAGC 1 cut(s) 394
Bsh1236I CGCG 2 cut(s) 64, 165
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 3 cut(s) 162, 530, 697
BsiEI CGRYCG 1 cut(s) 90
BsiSI CCGG 3 cut(s) 86, 99, 781
BsiWI CGTACG 1 cut(s) 709
BslI CCNNNNNNNGG 3 cut(s) 537, 551, 769
BsmAI GTCTC 2 cut(s) 45, 161
BsmI GAATGC 1 cut(s) 444
BsnI GGCC 3 cut(s) 162, 530, 697
Bsp143I GATC 4 cut(s) 67, 225, 499, 759
Bsp1720I GCTNAGC 1 cut(s) 309
BspACI CCGC 3 cut(s) 62, 163, 486
BspANI GGCC 3 cut(s) 162, 530, 697
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 2 cut(s) 64, 165
BspHI TCATGA 1 cut(s) 210
BspPI GGATC 2 cut(s) 220, 754
BsrI ACTGG 3 cut(s) 163, 220, 791
BssECI CCNNGG 2 cut(s) 409, 681
BssMI GATC 4 cut(s) 67, 225, 499, 759
BssNI GRCGYC 1 cut(s) 197
BssT1I CCWWGG 1 cut(s) 681
Bst2UI CCWGG 1 cut(s) 410
Bst4CI ACNGT 1 cut(s) 772
BstACI GRCGYC 1 cut(s) 197
BstBAI YACGTR 1 cut(s) 548
BstC8I GCNNGC 2 cut(s) 43, 513
BstDEI CTNAG 1 cut(s) 309
BstF5I GGATG 4 cut(s) 224, 289, 541, 691
BstFNI CGCG 2 cut(s) 64, 165
BstKTI GATC 4 cut(s) 70, 228, 502, 762
BstMAI GTCTC 2 cut(s) 45, 161
BstMBI GATC 4 cut(s) 67, 225, 499, 759
BstMCI CGRYCG 1 cut(s) 90
BstNI CCWGG 1 cut(s) 410
BstNSI RCATGY 1 cut(s) 748
BstSCI CCNGG 2 cut(s) 408, 779
BstUI CGCG 2 cut(s) 64, 165
BstV1I GCAGC 1 cut(s) 57
BstV2I GAAGAC 3 cut(s) 114, 120, 126
BstX2I RGATCY 1 cut(s) 759
BstXI CCANNNNNNTGG 1 cut(s) 158
BstYI RGATCY 1 cut(s) 759
BsuRI GGCC 3 cut(s) 162, 530, 697
BtgZI GCGATG 1 cut(s) 117
BtsCI GGATG 4 cut(s) 224, 289, 541, 691
BtsIMutI CAGTG 2 cut(s) 156, 798
Cac8I GCNNGC 2 cut(s) 43, 513
CaiI CAGNNNCTG 1 cut(s) 763
CciI TCATGA 1 cut(s) 210
CseI GACGC 1 cut(s) 186
Csp6I GTAC 1 cut(s) 710
CviAII CATG 4 cut(s) 211, 382, 390, 745
CviQI GTAC 1 cut(s) 710
DdeI CTNAG 1 cut(s) 309
DpnI GATC 4 cut(s) 69, 227, 501, 761
DpnII GATC 4 cut(s) 67, 225, 499, 759
DraIII CACNNNGTG 1 cut(s) 280
EaeI YGGCCR 2 cut(s) 160, 528
EciI GGCGGA 1 cut(s) 475
Eco130I CCWWGG 1 cut(s) 681
Eco57I CTGAAG 1 cut(s) 578
EcoRII CCWGG 1 cut(s) 408
EcoT14I CCWWGG 1 cut(s) 681
EcoT22I ATGCAT 1 cut(s) 565
ErhI CCWWGG 1 cut(s) 681
FaeI CATG 4 cut(s) 214, 385, 393, 748
FatI CATG 4 cut(s) 210, 381, 389, 744
Fnu4HI GCNGC 3 cut(s) 46, 62, 163
FokI GGATG 4 cut(s) 231, 296, 528, 698
Fsp4HI GCNGC 3 cut(s) 46, 62, 163
FspBI CTAG 1 cut(s) 314
GluI GCNGC 3 cut(s) 46, 62, 163
GsaI CCCAGC 1 cut(s) 398
GsuI CTGGAG 1 cut(s) 759
HaeIII GGCC 3 cut(s) 162, 530, 697
HapII CCGG 3 cut(s) 86, 99, 781
HgaI GACGC 1 cut(s) 186
Hin1I GRCGYC 1 cut(s) 197
Hin1II CATG 4 cut(s) 214, 385, 393, 748
HincII GTYRAC 1 cut(s) 768
HindII GTYRAC 1 cut(s) 768
HinfI GANTC 4 cut(s) 95, 318, 339, 776
HpaII CCGG 3 cut(s) 86, 99, 781
HphI GGTGA 3 cut(s) 431, 620, 671
Hpy166II GTNNAC 1 cut(s) 768
Hpy188I TCNGA 1 cut(s) 597
Hpy188III TCNNGA 3 cut(s) 71, 211, 738
Hpy8I GTNNAC 1 cut(s) 768
Hpy99I CGWCG 3 cut(s) 22, 25, 28
HpyAV CCTTC 2 cut(s) 526, 602
HpyCH4III ACNGT 1 cut(s) 772
HpyCH4IV ACGT 2 cut(s) 260, 547
HpyCH4V TGCA 2 cut(s) 401, 563
HpyF3I CTNAG 1 cut(s) 309
HpySE526I ACGT 2 cut(s) 260, 547
Hsp92I GRCGYC 1 cut(s) 197
Hsp92II CATG 4 cut(s) 214, 385, 393, 748
Kzo9I GATC 4 cut(s) 67, 225, 499, 759
Lsp1109I GCAGC 1 cut(s) 57
LweI GCATC 3 cut(s) 209, 410, 550
MaeI CTAG 1 cut(s) 314
MaeII ACGT 2 cut(s) 260, 547
MaeIII GTNAC 2 cut(s) 343, 586
MalI GATC 4 cut(s) 69, 227, 501, 761
MboI GATC 4 cut(s) 67, 225, 499, 759
MboII GAAGA 5 cut(s) 108, 119, 120, 126, 283
MfeI CAATTG 1 cut(s) 30
MflI RGATCY 1 cut(s) 759
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 5 cut(s) 30, 617, 700, 720, 726
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 1 cut(s) 89
MnlI CCTC 7 cut(s) 151, 154, 164, 478, 607, 709, 734
Mox20I TGGCCA 1 cut(s) 530
Mph1103I ATGCAT 1 cut(s) 565
MscI TGGCCA 1 cut(s) 530
MslI CAYNNNNRTG 1 cut(s) 749
Msp20I TGGCCA 1 cut(s) 530
MspI CCGG 3 cut(s) 86, 99, 781
MspR9I CCNGG 2 cut(s) 410, 781
MunI CAATTG 1 cut(s) 30
Mva1269I GAATGC 1 cut(s) 444
MvaI CCWGG 1 cut(s) 410
MvnI CGCG 2 cut(s) 64, 165
NciI CCSGG 1 cut(s) 781
NdeII GATC 4 cut(s) 67, 225, 499, 759
NlaIII CATG 4 cut(s) 214, 385, 393, 748
NsiI ATGCAT 1 cut(s) 565
NspI RCATGY 1 cut(s) 748
PagI TCATGA 1 cut(s) 210
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 1 cut(s) 444
PfeI GAWTC 3 cut(s) 318, 339, 776
Pfl23II CGTACG 1 cut(s) 709
PflMI CCANNNNNTGG 1 cut(s) 551
PfoI TCCNGGA 1 cut(s) 779
PkrI GCNGC 3 cut(s) 47, 63, 164
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Ppu21I YACGTR 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 408
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 1 cut(s) 408
PspLI CGTACG 1 cut(s) 709
PstNI CAGNNNCTG 1 cut(s) 763
PsuI RGATCY 1 cut(s) 759
RsaI GTAC 1 cut(s) 711
RsaNI GTAC 1 cut(s) 710
RseI CAYNNNNRTG 1 cut(s) 749
SatI GCNGC 3 cut(s) 46, 62, 163
Sau3AI GATC 4 cut(s) 67, 225, 499, 759
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 410, 781
SetI ASST 7 cut(s) 78, 114, 156, 263, 315, 550, 803
SfaNI GCATC 3 cut(s) 209, 410, 550
SmiMI CAYNNNNRTG 1 cut(s) 749
Sse9I AATT 5 cut(s) 30, 617, 700, 720, 726
SsiI CCGC 3 cut(s) 62, 163, 486
SspMI CTAG 1 cut(s) 314
StyD4I CCNGG 2 cut(s) 408, 779
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 1 cut(s) 772
TaiI ACGT 2 cut(s) 263, 550
TaqI TCGA 1 cut(s) 70
TaqII GACCGA 1 cut(s) 320
TasI AATT 5 cut(s) 30, 617, 700, 720, 726
TauI GCSGC 2 cut(s) 64, 165
TfiI GAWTC 3 cut(s) 318, 339, 776
TscAI CASTG 2 cut(s) 163, 798
TseI GCWGC 1 cut(s) 45
TspDTI ATGAA 6 cut(s) 120, 199, 349, 370, 562, 570
TspGWI ACGGA 1 cut(s) 697
TspRI CASTG 2 cut(s) 163, 798
Van91I CCANNNNNTGG 1 cut(s) 551
XapI RAATTY 1 cut(s) 700
XceI RCATGY 1 cut(s) 748
XcmI CCANNNNNNNNNTGG 1 cut(s) 653
XspI CTAG 1 cut(s) 314
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.