Rh2DG051200

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008495
Physical Location & Seq
Forward (+)
3658721 .. 3662118
3398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG051200.1

Sequence Viewer

Length: 813 bp
ATGGGCGATGAGAAGTCATCGTCGTCGTCGTCGTCGTCAATTGTAATGGCAAGCAGTAGAGACAGAGAGCCGCGAGATCGAGAGCTTCTCATTCCGGTCGCCGACTCCGGCGATGAAGACCTTTCTTCCAAGCCCTCTTCGTCTTCGTCCTCCTCGCACCACCTCACTGGCCGCGAGACTTTTTACAAAGTTTTTAGAAGTTGGGCGTCAAAGAAGTTCATGACTGGATGCGTGATCCTATTTCCAATAGCCATCACTTTCTACATAACTTGGTGGTTTATTCACTTTGTGGATGGCTTCTTCTCTCCAATATATGCTCAGCTAGGAATCAACATTTTCGGTCTTGGATTTGTAACTTCCATTACGTTCATATTTTTGGTTGGGGTGTTCATGTCATCATGGCTGGGTGCATCTGTCCTGGGTCTCGGTGAGTGGTTTATCAAAAGAATGCCATTCGTTCGTCATATCTACAATGCCTCCAAGCAGATTAGTTCTGCCATATCCCCAGATCAAAACACGCAAGCCTTCAAGGAAGTGGCCATCATAAGGCATCCACGTATTGGAGAATATGCATTTGGGTTCATTACTTCATCAGTTACCCTTCAGAACTATGCTGGTGATGAGGAATTGTGCTGTGTTTATGTTCCCACGAACCATTTGTATATTGGTGATGTCTTTCTGGTCAGCACCAAGGATGTGATCAGGCCAAATTTATCCGTACGGGAAGGAATTGAGATTGTTGTGTCTGGAGGCATGTCGATGCCCCAGATCCTGTCAACGGTTGATTCCCGGATTATGCCAGTGGTTAGCTGA

Protein Analysis

270

Amino Acids

29.99

Weight (kDa)

6.3

Isoelectric Point (pI)

54.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 119 - 219 1.7e-27 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 560
AccII CGCG 2 cut(s) 73, 174
AciI CCGC 2 cut(s) 71, 172
AclWI GGATC 2 cut(s) 229, 763
AcoI YGGCCR 2 cut(s) 169, 537
AcsI RAATTY 1 cut(s) 709
AcuI CTGAAG 1 cut(s) 587
AcyI GRCGYC 1 cut(s) 206
AdeI CACNNNGTG 1 cut(s) 289
AfaI GTAC 1 cut(s) 720
AfiI CCNNNNNNNGG 3 cut(s) 546, 560, 778
AgsI TTSAA 1 cut(s) 529
AjnI CCWGG 1 cut(s) 417
AjuI GAANNNNNNNTTGG 2 cut(s) 558, 590
AluBI AGCT 3 cut(s) 85, 322, 810
AluI AGCT 3 cut(s) 85, 322, 810
Alw26I GTCTC 3 cut(s) 54, 170, 428
AlwI GGATC 2 cut(s) 229, 763
AlwNI CAGNNNCTG 1 cut(s) 772
AoxI GGCC 3 cut(s) 169, 537, 704
ApoI RAATTY 1 cut(s) 709
AsuC2I CCSGG 1 cut(s) 790
AsuHPI GGTGA 3 cut(s) 440, 629, 680
BalI TGGCCA 1 cut(s) 539
BbsI GAAGAC 2 cut(s) 123, 135
BccI CCATC 3 cut(s) 260, 287, 548
BciT130I CCWGG 1 cut(s) 419
BclI TGATCA 1 cut(s) 699
BcnI CCSGG 1 cut(s) 790
BcoDI GTCTC 3 cut(s) 54, 170, 428
BfaI CTAG 1 cut(s) 323
BisI GCNGC 2 cut(s) 71, 172
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 2 cut(s) 72, 173
Bme1390I CCNGG 2 cut(s) 419, 790
BmrFI CCNGG 2 cut(s) 419, 790
BmsI GCATC 4 cut(s) 218, 419, 559, 750
BpiI GAAGAC 2 cut(s) 123, 135
BplI GAGNNNNNCTC 2 cut(s) 72, 104
BpmI CTGGAG 1 cut(s) 768
Bpu1102I GCTNAGC 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 790
BsaAI YACGTR 1 cut(s) 557
BsaHI GRCGYC 1 cut(s) 206
BsaI GGTCTC 1 cut(s) 428
BsaJI CCNNGG 2 cut(s) 418, 690
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bsc4I CCNNNNNNNGG 3 cut(s) 546, 560, 778
Bse1I ACTGG 3 cut(s) 172, 229, 800
BseBI CCWGG 1 cut(s) 419
BseDI CCNNGG 2 cut(s) 418, 690
BseGI GGATG 4 cut(s) 233, 298, 550, 700
BseLI CCNNNNNNNGG 3 cut(s) 546, 560, 778
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 3 cut(s) 172, 229, 800
BseRI GAGGAG 1 cut(s) 142
BseYI CCCAGC 1 cut(s) 403
Bsh1236I CGCG 2 cut(s) 73, 174
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 3 cut(s) 171, 539, 706
BsiEI CGRYCG 1 cut(s) 99
BsiSI CCGG 3 cut(s) 95, 108, 790
BsiWI CGTACG 1 cut(s) 718
BslI CCNNNNNNNGG 3 cut(s) 546, 560, 778
BsmAI GTCTC 3 cut(s) 54, 170, 428
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 3 cut(s) 171, 539, 706
Bso31I GGTCTC 1 cut(s) 428
Bsp143I GATC 5 cut(s) 76, 234, 508, 699, 768
Bsp1720I GCTNAGC 1 cut(s) 318
BspACI CCGC 2 cut(s) 71, 172
BspANI GGCC 3 cut(s) 171, 539, 706
BspCNI CTCAG 1 cut(s) 331
BspFNI CGCG 2 cut(s) 73, 174
BspHI TCATGA 1 cut(s) 219
BspPI GGATC 2 cut(s) 229, 763
BspTNI GGTCTC 1 cut(s) 428
BsrI ACTGG 3 cut(s) 172, 229, 800
BssECI CCNNGG 2 cut(s) 418, 690
BssMI GATC 5 cut(s) 76, 234, 508, 699, 768
BssNI GRCGYC 1 cut(s) 206
BssT1I CCWWGG 1 cut(s) 690
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 1 cut(s) 142
BstACI GRCGYC 1 cut(s) 206
BstBAI YACGTR 1 cut(s) 557
BstC8I GCNNGC 2 cut(s) 52, 522
BstDEI CTNAG 1 cut(s) 318
BstF5I GGATG 4 cut(s) 233, 298, 550, 700
BstFNI CGCG 2 cut(s) 73, 174
BstKTI GATC 5 cut(s) 79, 237, 511, 702, 771
BstMAI GTCTC 3 cut(s) 54, 170, 428
BstMBI GATC 5 cut(s) 76, 234, 508, 699, 768
BstMCI CGRYCG 1 cut(s) 99
BstNI CCWGG 1 cut(s) 419
BstNSI RCATGY 1 cut(s) 757
BstSCI CCNGG 2 cut(s) 417, 788
BstUI CGCG 2 cut(s) 73, 174
BstV2I GAAGAC 2 cut(s) 123, 135
BstX2I RGATCY 1 cut(s) 768
BstXI CCANNNNNNTGG 1 cut(s) 167
BstYI RGATCY 1 cut(s) 768
BsuRI GGCC 3 cut(s) 171, 539, 706
BtgZI GCGATG 2 cut(s) 21, 126
BtsCI GGATG 4 cut(s) 233, 298, 550, 700
BtsIMutI CAGTG 2 cut(s) 165, 807
Cac8I GCNNGC 2 cut(s) 52, 522
CaiI CAGNNNCTG 1 cut(s) 772
CciI TCATGA 1 cut(s) 219
CseI GACGC 1 cut(s) 195
Csp6I GTAC 1 cut(s) 719
CviAII CATG 4 cut(s) 220, 391, 399, 754
CviQI GTAC 1 cut(s) 719
DdeI CTNAG 1 cut(s) 318
DpnI GATC 5 cut(s) 78, 236, 510, 701, 770
DpnII GATC 5 cut(s) 76, 234, 508, 699, 768
DraIII CACNNNGTG 1 cut(s) 289
EaeI YGGCCR 2 cut(s) 169, 537
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
Eco130I CCWWGG 1 cut(s) 690
Eco31I GGTCTC 1 cut(s) 428
Eco57I CTGAAG 1 cut(s) 587
EcoRII CCWGG 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 690
EcoT22I ATGCAT 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 690
FaeI CATG 4 cut(s) 223, 394, 402, 757
FatI CATG 4 cut(s) 219, 390, 398, 753
FbaI TGATCA 1 cut(s) 699
Fnu4HI GCNGC 2 cut(s) 71, 172
FokI GGATG 4 cut(s) 240, 305, 537, 707
Fsp4HI GCNGC 2 cut(s) 71, 172
FspBI CTAG 1 cut(s) 323
GluI GCNGC 2 cut(s) 71, 172
GsaI CCCAGC 1 cut(s) 407
GsuI CTGGAG 1 cut(s) 768
HaeIII GGCC 3 cut(s) 171, 539, 706
HapII CCGG 3 cut(s) 95, 108, 790
HgaI GACGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 4 cut(s) 223, 394, 402, 757
HincII GTYRAC 1 cut(s) 777
HindII GTYRAC 1 cut(s) 777
HinfI GANTC 3 cut(s) 104, 327, 785
HpaII CCGG 3 cut(s) 95, 108, 790
HphI GGTGA 3 cut(s) 440, 629, 680
Hpy166II GTNNAC 1 cut(s) 777
Hpy188I TCNGA 1 cut(s) 606
Hpy188III TCNNGA 3 cut(s) 80, 220, 747
Hpy8I GTNNAC 1 cut(s) 777
Hpy99I CGWCG 5 cut(s) 25, 28, 31, 34, 37
HpyAV CCTTC 3 cut(s) 535, 611, 719
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4IV ACGT 2 cut(s) 365, 556
HpyCH4V TGCA 2 cut(s) 410, 572
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 2 cut(s) 365, 556
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 4 cut(s) 223, 394, 402, 757
Ksp22I TGATCA 1 cut(s) 699
Kzo9I GATC 5 cut(s) 76, 234, 508, 699, 768
LweI GCATC 4 cut(s) 218, 419, 559, 750
MaeI CTAG 1 cut(s) 323
MaeII ACGT 2 cut(s) 365, 556
MaeIII GTNAC 2 cut(s) 352, 595
MalI GATC 5 cut(s) 78, 236, 510, 701, 770
MboI GATC 5 cut(s) 76, 234, 508, 699, 768
MboII GAAGA 5 cut(s) 117, 128, 129, 135, 292
MfeI CAATTG 1 cut(s) 39
MflI RGATCY 1 cut(s) 768
MlsI TGGCCA 1 cut(s) 539
MluCI AATT 4 cut(s) 39, 626, 709, 729
MluNI TGGCCA 1 cut(s) 539
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 7 cut(s) 145, 160, 163, 173, 487, 616, 743
Mox20I TGGCCA 1 cut(s) 539
Mph1103I ATGCAT 1 cut(s) 574
MscI TGGCCA 1 cut(s) 539
MslI CAYNNNNRTG 1 cut(s) 758
Msp20I TGGCCA 1 cut(s) 539
MspI CCGG 3 cut(s) 95, 108, 790
MspR9I CCNGG 2 cut(s) 419, 790
MunI CAATTG 1 cut(s) 39
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 1 cut(s) 419
MvnI CGCG 2 cut(s) 73, 174
NciI CCSGG 1 cut(s) 790
NdeII GATC 5 cut(s) 76, 234, 508, 699, 768
NlaIII CATG 4 cut(s) 223, 394, 402, 757
NsiI ATGCAT 1 cut(s) 574
NspI RCATGY 1 cut(s) 757
PagI TCATGA 1 cut(s) 219
PcsI WCGNNNNNNNCGW 3 cut(s) 26, 29, 32
PctI GAATGC 1 cut(s) 453
PfeI GAWTC 2 cut(s) 327, 785
Pfl23II CGTACG 1 cut(s) 718
PflMI CCANNNNNTGG 1 cut(s) 560
PfoI TCCNGGA 1 cut(s) 788
PkrI GCNGC 2 cut(s) 72, 173
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Ppu21I YACGTR 1 cut(s) 557
Psp6I CCWGG 1 cut(s) 417
PspFI CCCAGC 1 cut(s) 403
PspGI CCWGG 1 cut(s) 417
PspLI CGTACG 1 cut(s) 718
PstNI CAGNNNCTG 1 cut(s) 772
PsuI RGATCY 1 cut(s) 768
RsaI GTAC 1 cut(s) 720
RsaNI GTAC 1 cut(s) 719
RseI CAYNNNNRTG 1 cut(s) 758
SatI GCNGC 2 cut(s) 71, 172
Sau3AI GATC 5 cut(s) 76, 234, 508, 699, 768
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 2 cut(s) 419, 790
SetI ASST 7 cut(s) 87, 123, 165, 324, 368, 559, 812
SfaNI GCATC 4 cut(s) 218, 419, 559, 750
SmiMI CAYNNNNRTG 1 cut(s) 758
Sse9I AATT 4 cut(s) 39, 626, 709, 729
SsiI CCGC 2 cut(s) 71, 172
SspMI CTAG 1 cut(s) 323
StyD4I CCNGG 2 cut(s) 417, 788
StyI CCWWGG 1 cut(s) 690
TaaI ACNGT 1 cut(s) 781
TaiI ACGT 2 cut(s) 368, 559
TaqI TCGA 2 cut(s) 79, 758
TaqII GACCGA 1 cut(s) 329
TasI AATT 4 cut(s) 39, 626, 709, 729
TauI GCSGC 2 cut(s) 73, 174
TfiI GAWTC 2 cut(s) 327, 785
TscAI CASTG 2 cut(s) 172, 807
TspDTI ATGAA 6 cut(s) 129, 208, 358, 379, 571, 579
TspGWI ACGGA 1 cut(s) 706
TspRI CASTG 2 cut(s) 172, 807
Van91I CCANNNNNTGG 1 cut(s) 560
XapI RAATTY 1 cut(s) 709
XceI RCATGY 1 cut(s) 757
XcmI CCANNNNNNNNNTGG 1 cut(s) 662
XspI CTAG 1 cut(s) 323
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.