MD02G1044400.v1.1

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008495
Physical Location & Seq
Forward (+)
3622884 .. 3626007
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1044400.v1.1.491

Sequence Viewer

Length: 807 bp
ATGGGCGATGAGAAGTCGTCGTCTTCGATCGTAATGGCGAGCAGAGACAGGGAACCGCGGGATCGTGAGCTTCTCATTCCGGTGGAGGACTCCGGCGACGAAGATGCATCGTCCAAACCCTCCTCGTCTTCCTCGTCATCCCAGCACACCGGCCGCGAGACATTTTCCAAAGTTGTTAGAAGTTGGGCATCAAAAAAGTTCATGACAGGATGTGTGATCTTATTTCCAATAGCGATCACTTTCTATATCACATGGTGGTTTATTCACTTTGTGGATGGTTTCTTCTCTCCGATCTATGCTCAACTTGGAATCGACATTTTTGGTCTTGGATTTGTAACTTCTATTACGTTCATATTTATCGTTGGGGTGTTCATGTCATCATGGTTGGGTGCATCTGTCCTGGGCCTTGGAGAGTGGTTTATCAAAAGAATGCCATTTGTTCGTCATATCTACAATGCCTCCAAGCAGATTAGTTCTGCTATATCCCCAGATCAAAATACACAAGCTTTCAAGGAAGTGGCCATAATAAGGCATCCACGTATTGGTGAATATGCATTCGGGTTCATCACTTCATCCGTTGTCCTTCAGAGCTATTCTGGGGATGAAGAGTTGTGCTGTGTTTATGTTCCCACGAACCATCTTTACATTGGTGATGTATTCCTGGTCAACACCAAAGATGTTATCAGACCAAATTTATCTGTACGGGAGGGAATTGAAATTGTTGTGTCTGGAGGCATGTCAATGCCCCAGATCCTGTCAACGCTTGATCCACGGATCATGCCAGTGGAAGCTGAGGGAAGGCGTTAG

Protein Analysis

269

Amino Acids

29.88

Weight (kDa)

5.76

Isoelectric Point (pI)

51.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 113 - 213 4.9e-27 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 542
AccII CGCG 2 cut(s) 58, 156
AciI CCGC 3 cut(s) 56, 58, 154
AclWI GGATC 4 cut(s) 69, 745, 761, 782
AcoI YGGCCR 2 cut(s) 151, 519
AcsI RAATTY 1 cut(s) 691
AcuI CTGAAG 1 cut(s) 569
AdeI CACNNNGTG 2 cut(s) 255, 271
AfaI GTAC 1 cut(s) 702
AfiI CCNNNNNNNGG 2 cut(s) 528, 542
AgsI TTSAA 2 cut(s) 511, 716
AjnI CCWGG 2 cut(s) 399, 660
AluBI AGCT 4 cut(s) 70, 506, 591, 791
AluI AGCT 4 cut(s) 70, 506, 591, 791
Alw26I GTCTC 2 cut(s) 39, 152
AlwI GGATC 4 cut(s) 69, 745, 761, 782
AlwNI CAGNNNCTG 1 cut(s) 754
AoxI GGCC 3 cut(s) 151, 403, 519
ApoI RAATTY 1 cut(s) 691
AspS9I GGNCC 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 557, 662
BalI TGGCCA 1 cut(s) 521
BbsI GAAGAC 2 cut(s) 15, 120
BbvCI CCTCAGC 1 cut(s) 792
BccI CCATC 2 cut(s) 269, 645
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 2 cut(s) 401, 662
BcoDI GTCTC 2 cut(s) 39, 152
BisI GCNGC 1 cut(s) 154
BlsI GCNGC 1 cut(s) 155
Bme1390I CCNGG 2 cut(s) 401, 662
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 2 cut(s) 401, 662
BmsI GCATC 5 cut(s) 94, 116, 197, 401, 541
BpiI GAAGAC 2 cut(s) 15, 120
BpmI CTGGAG 1 cut(s) 750
Bpu10I CCTNAGC 1 cut(s) 792
BsaAI YACGTR 1 cut(s) 539
BsaJI CCNNGG 4 cut(s) 56, 400, 406, 770
BsaWI WCCGGW 1 cut(s) 79
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 2 cut(s) 528, 542
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 782
BseBI CCWGG 2 cut(s) 401, 662
BseDI CCNNGG 4 cut(s) 56, 400, 406, 770
BseGI GGATG 6 cut(s) 137, 215, 280, 532, 572, 607
BseLI CCNNNNNNNGG 2 cut(s) 528, 542
BseMII CTCAG 1 cut(s) 783
BseNI ACTGG 1 cut(s) 782
BseRI GAGGAG 1 cut(s) 112
BseX3I CGGCCG 1 cut(s) 151
BseYI CCCAGC 1 cut(s) 141
Bsh1236I CGCG 2 cut(s) 58, 156
Bsh1285I CGRYCG 2 cut(s) 30, 154
BshFI GGCC 3 cut(s) 153, 405, 521
BsiEI CGRYCG 2 cut(s) 30, 154
BsiSI CCGG 3 cut(s) 80, 93, 150
BslI CCNNNNNNNGG 2 cut(s) 528, 542
BsmAI GTCTC 2 cut(s) 39, 152
BsmI GAATGC 2 cut(s) 435, 554
BsnI GGCC 3 cut(s) 153, 405, 521
Bsp143I GATC 9 cut(s) 27, 61, 216, 234, 291, 490, 750, 766, 774
BspACI CCGC 3 cut(s) 56, 58, 154
BspANI GGCC 3 cut(s) 153, 405, 521
BspCNI CTCAG 1 cut(s) 784
BspFNI CGCG 2 cut(s) 58, 156
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 54
BspPI GGATC 4 cut(s) 69, 745, 761, 782
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 1 cut(s) 782
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 4 cut(s) 56, 400, 406, 770
BssMI GATC 9 cut(s) 27, 61, 216, 234, 291, 490, 750, 766, 774
BssT1I CCWWGG 1 cut(s) 406
Bst2UI CCWGG 2 cut(s) 401, 662
Bst6I CTCTTC 1 cut(s) 600
BstBAI YACGTR 1 cut(s) 539
BstC8I GCNNGC 1 cut(s) 40
BstDEI CTNAG 1 cut(s) 792
BstDSI CCRYGG 2 cut(s) 56, 770
BstF5I GGATG 6 cut(s) 137, 215, 280, 532, 572, 607
BstFNI CGCG 2 cut(s) 58, 156
BstKTI GATC 9 cut(s) 30, 64, 219, 237, 294, 493, 753, 769, 777
BstMAI GTCTC 2 cut(s) 39, 152
BstMBI GATC 9 cut(s) 27, 61, 216, 234, 291, 490, 750, 766, 774
BstMCI CGRYCG 2 cut(s) 30, 154
BstNI CCWGG 2 cut(s) 401, 662
BstNSI RCATGY 1 cut(s) 739
BstSCI CCNGG 2 cut(s) 399, 660
BstUI CGCG 2 cut(s) 58, 156
BstV2I GAAGAC 2 cut(s) 15, 120
BstX2I RGATCY 1 cut(s) 750
BstYI RGATCY 1 cut(s) 750
BstZI CGGCCG 1 cut(s) 151
BsuRI GGCC 3 cut(s) 153, 405, 521
BtgI CCRYGG 2 cut(s) 56, 770
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 6 cut(s) 137, 215, 280, 532, 572, 607
BtsIMutI CAGTG 1 cut(s) 789
Cac8I GCNNGC 1 cut(s) 40
CaiI CAGNNNCTG 1 cut(s) 754
CciI TCATGA 1 cut(s) 201
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 403
Cfr42I CCGCGG 1 cut(s) 59
Csp6I GTAC 1 cut(s) 701
CviAII CATG 6 cut(s) 202, 252, 373, 381, 736, 778
CviJI RGCY 7 cut(s) 70, 153, 405, 506, 521, 591, 791
CviKI_1 RGCY 7 cut(s) 70, 153, 405, 506, 521, 591, 791
CviQI GTAC 1 cut(s) 701
DdeI CTNAG 1 cut(s) 792
DpnI GATC 9 cut(s) 29, 63, 218, 236, 293, 492, 752, 768, 776
DpnII GATC 9 cut(s) 27, 61, 216, 234, 291, 490, 750, 766, 774
DraIII CACNNNGTG 2 cut(s) 255, 271
EaeI YGGCCR 2 cut(s) 151, 519
EagI CGGCCG 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 600
EarI CTCTTC 1 cut(s) 600
EclXI CGGCCG 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 406
Eco52I CGGCCG 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 569
EcoRII CCWGG 2 cut(s) 399, 660
EcoT14I CCWWGG 1 cut(s) 406
EcoT22I ATGCAT 2 cut(s) 109, 556
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 6 cut(s) 205, 255, 376, 384, 739, 781
FatI CATG 6 cut(s) 201, 251, 372, 380, 735, 777
FauI CCCGC 1 cut(s) 51
Fnu4HI GCNGC 1 cut(s) 154
FokI GGATG 6 cut(s) 124, 222, 287, 519, 559, 614
Fsp4HI GCNGC 1 cut(s) 154
GluI GCNGC 1 cut(s) 154
GsaI CCCAGC 1 cut(s) 145
GsuI CTGGAG 1 cut(s) 750
HaeIII GGCC 3 cut(s) 153, 405, 521
HapII CCGG 3 cut(s) 80, 93, 150
Hin1II CATG 6 cut(s) 205, 255, 376, 384, 739, 781
HincII GTYRAC 2 cut(s) 667, 759
HindII GTYRAC 2 cut(s) 667, 759
HindIII AAGCTT 1 cut(s) 504
HinfI GANTC 2 cut(s) 89, 309
HpaII CCGG 3 cut(s) 80, 93, 150
HphI GGTGA 2 cut(s) 557, 662
Hpy166II GTNNAC 2 cut(s) 667, 759
Hpy188I TCNGA 3 cut(s) 291, 588, 686
Hpy188III TCNNGA 3 cut(s) 65, 202, 729
Hpy8I GTNNAC 2 cut(s) 667, 759
Hpy99I CGWCG 2 cut(s) 22, 101
HpyAV CCTTC 2 cut(s) 593, 792
HpyCH4IV ACGT 2 cut(s) 347, 538
HpyCH4V TGCA 3 cut(s) 107, 392, 554
HpyF3I CTNAG 1 cut(s) 792
HpySE526I ACGT 2 cut(s) 347, 538
Hsp92II CATG 6 cut(s) 205, 255, 376, 384, 739, 781
KspI CCGCGG 1 cut(s) 59
Kzo9I GATC 9 cut(s) 27, 61, 216, 234, 291, 490, 750, 766, 774
LweI GCATC 5 cut(s) 94, 116, 197, 401, 541
MaeII ACGT 2 cut(s) 347, 538
MaeIII GTNAC 1 cut(s) 334
MalI GATC 9 cut(s) 29, 63, 218, 236, 293, 492, 752, 768, 776
MboI GATC 9 cut(s) 27, 61, 216, 234, 291, 490, 750, 766, 774
MboII GAAGA 5 cut(s) 15, 113, 120, 274, 617
MflI RGATCY 1 cut(s) 750
MlsI TGGCCA 1 cut(s) 521
MluCI AATT 3 cut(s) 691, 711, 717
MluNI TGGCCA 1 cut(s) 521
MlyI GAGTC 1 cut(s) 83
MnlI CCTC 8 cut(s) 79, 130, 133, 142, 469, 700, 725, 787
Mox20I TGGCCA 1 cut(s) 521
Mph1103I ATGCAT 2 cut(s) 109, 556
MscI TGGCCA 1 cut(s) 521
MslI CAYNNNNRTG 3 cut(s) 80, 740, 782
Msp20I TGGCCA 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 3 cut(s) 80, 93, 150
MspR9I CCNGG 2 cut(s) 401, 662
Mva1269I GAATGC 2 cut(s) 435, 554
MvaI CCWGG 2 cut(s) 401, 662
MvnI CGCG 2 cut(s) 58, 156
NdeII GATC 9 cut(s) 27, 61, 216, 234, 291, 490, 750, 766, 774
NlaIII CATG 6 cut(s) 205, 255, 376, 384, 739, 781
NlaIV GGNNCC 1 cut(s) 54
NsiI ATGCAT 2 cut(s) 109, 556
NspI RCATGY 1 cut(s) 739
PagI TCATGA 1 cut(s) 201
PcsI WCGNNNNNNNCGW 2 cut(s) 23, 131
PctI GAATGC 2 cut(s) 435, 554
PfeI GAWTC 1 cut(s) 309
PflMI CCANNNNNTGG 1 cut(s) 542
PkrI GCNGC 1 cut(s) 155
Ple19I CGATCG 1 cut(s) 30
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
Ppu21I YACGTR 1 cut(s) 539
Psp6I CCWGG 2 cut(s) 399, 660
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 2 cut(s) 399, 660
PspN4I GGNNCC 1 cut(s) 54
PspPI GGNCC 1 cut(s) 403
PsrI GAACNNNNNNTAC 2 cut(s) 626, 658
PstNI CAGNNNCTG 1 cut(s) 754
PsuI RGATCY 1 cut(s) 750
PvuI CGATCG 1 cut(s) 30
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
RseI CAYNNNNRTG 3 cut(s) 80, 740, 782
SacII CCGCGG 1 cut(s) 59
SatI GCNGC 1 cut(s) 154
Sau3AI GATC 9 cut(s) 27, 61, 216, 234, 291, 490, 750, 766, 774
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 2 cut(s) 401, 662
SetI ASST 6 cut(s) 72, 350, 508, 541, 593, 793
SfaNI GCATC 5 cut(s) 94, 116, 197, 401, 541
Sfr303I CCGCGG 1 cut(s) 59
SgrBI CCGCGG 1 cut(s) 59
SmiMI CAYNNNNRTG 3 cut(s) 80, 740, 782
Sse9I AATT 3 cut(s) 691, 711, 717
SsiI CCGC 3 cut(s) 56, 58, 154
StyD4I CCNGG 2 cut(s) 399, 660
StyI CCWWGG 1 cut(s) 406
TaiI ACGT 2 cut(s) 350, 541
TaqI TCGA 2 cut(s) 26, 312
TasI AATT 3 cut(s) 691, 711, 717
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 1 cut(s) 309
TscAI CASTG 1 cut(s) 789
TspDTI ATGAA 6 cut(s) 190, 340, 361, 553, 561, 618
TspGWI ACGGA 2 cut(s) 565, 787
TspRI CASTG 1 cut(s) 789
Van91I CCANNNNNTGG 1 cut(s) 542
XapI RAATTY 1 cut(s) 691
XceI RCATGY 1 cut(s) 739
XcmI CCANNNNNNNNNTGG 1 cut(s) 644
Zsp2I ATGCAT 2 cut(s) 109, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.