Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002217

Physical Location & Seq

Genomic Coordinates Reverse (-)

12852329 .. 12857727

Gene Structure 5399 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G30110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3243 bp

ATGCTTCACAAGCGAGCTAGTGAAGCTAACGATAAGAACGATAATACCATCATCGGTTCCGATTTAGCATCATCTAAGAAACGCCGTATCGATTTCACTGAATCTTCGTCTGATAAGTCTTCTTCGATTTTAGCTTCTGGTAGTAGCAGGGGTTTTCACGGCGATAGCGTCGTTCAGCAAATCGACATGGCTTTTGGTAATTCGAACCGTCAGGAGATTGATGAAGATCTGCACAGTCGCCAGCTCGCCGTCTATGGTCGTGAGACTATGAGGCGTCTCTTTGCTTCCAATGTTCTCATCTCGGGGATGCACGGTCTTGGTGCCGAGATTGCCAAGAATCTTATACTTGCTGGTGTGAAGTCTGTGACCTTGCATGATGAAAGAGTTGTAGAGCTATGGGACTTATCAAGCAACTTTGTTTTCTCGGAGGATGATGTTGGCAAGAATAGAGCAGATGCTTCTGTTCAGAAGTTACAGGATCTTAACAATGCTGTGGTTGTCTCTAGCTTGACCAAAAGCTTAAACAAAGAGGATCTTTCTGGCTTCCAGGTTGTTGTATTCTCTGACATAAGCATGGAAAGAGCAATCGAATTTGATGACTATTGTCACAGCCATCAGCCTCCTATCGCTTTTGTCAAGGCTGATGTCAGGGGTCTTTTCGGCTCTGTCTTTTGTGATTTTGGGCCTGAATTTGCGGTTCTTGATGTTGATGGAGAGGAACCCCATACAGGCATCATTGCGTCTATCTCTAATGAGAACCAGGCATTTATTTCCTGTGTCGACGATGAGAGGCTTGAGTTTGAAGATGGTGACCTAGTTGTTTTCTCAGAAGTTGAGGGTATGACAGAATTGAATGATGGGAAACCAAGGAAGATAAAAAGTACACGGCCATATTCATTCACCCTTGATGAGGACACTACTAACTATGGAACATATGTGAAGGGTGGTATTGTCACTCAAGTGAAACAGCCAAAGTTACTGAATTTCAAGCCATTGAGGGAAGCGCTTAAGGATCCAGGGGATTTTCTATTTAGTGATTTCTCTAAGTTTGATCGGCCTCCACTCCTTCACTTAGCATTCCAGGCACTTGATCATTTTAAAGCTGAAGCTGGCAGATTTCCTGTTGCTGGCTCAGAAGAGGACGCTCAGAAGCTTATATCGATTGCCACAGCCATCAATACCGGGCAGGGTGATTTAAAGGTGGAGAATGTTGATCAGAAGCTTCTAAGACACTTTTCCTTTGGAGCCAAGGCTGTCCTGAATCCTATGGCTGCAATGTTTGGCGGTATTGTTGGACAGGAGGTTGTTAAAGCTTGCTCTGGAAAATTTCATCCTCTCTTCCAGTTTTTCTACTTTGATTCAGTGGAGTCACTCCCCTCTGAACCTGTGGACTCGAGTGACTTTGCACCAAGAAACAGCCGGTATGATGCCCAAATATCTGTATTTGGGGCCAAGTTCCAGAAGAAACTTGAAGATGCTAAAGTTTTCACAGTAGGGTCTGGTGCTCTTGGCTGTGAGTTCTTGAAAAATTTGGCTCTGATGGGGGTTTCTTGTGGAAGCCAAGGGAAGCTGACAGTGACAGATGATGATATCATTGAGAAGAGTAACCTCAGTCGTCAATTTCTGTTCCGTGACTGGAACATTGGACAGGCTAAATCCACAGTTGCTGCTTCGGCTGCTGCTGTTATAAATCCTCGCTTCAACATTGAGGCCCTGCAAAACCGTGTTGGCGCGGAGACTGAGAATGTATTTGACGATGCCTTCTGGGAGAACTTAACTGTTGTCGTCAATGCACTGGATAATGTTAATGCGAGGCTCTATGTTGATTCGAGGTGCTTGTATTTCCAGAAGCCTCTCCTTGAGTCTGGAACTCTTGGTACAAAGTGCAACACACAGTCGGTTATCCCACATCTAACTGAAAATTACGGTGCCTCAAGGGACCCACCAGAGAAACAGGCCCCCATGTGTACAGTGCATTCCTTCCCGCATAACATTGATCACTGTTTAACTTGGGCTCGCTCTGAGTTTGAGGGTCTGCTGGAGAAGACTCCCGCTGAAGTGAATGCATATCTTTCTAGCCCAGTTGAGTACACTAACTCAATGATGAGTGCTGGTGATGCTCAGGCGAGGGACACATTGGAGAGGATTGTTGAGTGCCTTGAAAAGGAGAAGTGTGAGACCTTCCAAGACTGCTTAACCTGGGCACGACTGAGGTTTGAGGATTACTTTGTGAACCGTGTCAAGCAATTGATATACACATTTCCTGAAGATGCTGCGACTAGTACCGGAGCTCCATTCTGGTCTGCTCCAAAAAGATTCCCTCGTCCACTCCAGTACTCGTCGTCCGACCCAAGTCTCCTTAACTTTATCACAGCGACTGCTATTTTACGAGCAGAGACATTTGGGATCCCGATACCTGAGTGGACTAAGAACCCAAAGGAAGCAGCAGAAGCTGTTGACAGAGTGATAGTCCCAGACTTTGAGCCAAGGCAAGATGCAAAGATTGTGACTGATGAGAAAGCTACCACTTTAACCACTGCTTCGGTGGACGACGCTGCAGTCATCGACGATCTCATTGCTAAGATCGACCAGTGTAGGCATAACTTGTCGCCAGACTTCAGGATGAAACCTATTCAGTTCGAAAAGGATGATGATACAAACTATCACATGGATGTGATAGCAGGACTAGCCAACATGAGGGCGAGAAATTACAGCATCCCTGAAGTGGACAAGCTGAAAGCAAAGTTCATAGCAGGGAGAATCATCCCAGCCATTGCGACTTCAACAGCCATGGCTACTGGGCTGGTCTGCCTCGAGCTGTACAAGGTCCTTGATGGAGGACATAAAGTGGAAGCTTACAGAAACACATTTGCCAACCTGGCACTTCCACTATTCTCCATGGCTGAACCACTTCCACCAAAGGTGGTGAAGCACCGTGACATGGCTTGGACAGTTTGGGACAGATGGGTTCTCAAGGGAAACCCAACTCTTCGTGAGGTGTTGCAGTGGCTGGAGGACAAAGGGCTTAGCGCATACAGCATCTCTTGTGGAAGCTGTCTTCTGTTCAACAGTATGTTCACAAGGCACAAGGAGAGGATGGACAAGAAGGTTGTGGATCTCGCTCGGGATGTTGCGAAAGTCGAGTTACCGCCTTATCGCAACCATCTTGATGTAGTGGTGGCTTGTGAGGATGAAGATGACAATGACGTCGATATTCCTCTCGTCTCTATCTACTTCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030054 GO:0032446 GO:0033554 GO:0036211 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 ko05012 map04120 map05012

Pfam Domains

Protein Families

E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1080

Amino Acids

120.25

Weight (kDa)

5.11

Isoelectric Point (pI)

34.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	78 - 453	1.7e-25	ThiF family
E1_FCCH	PF16190	252 - 322	3.9e-28	Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB	PF16191	323 - 393	2.3e-26	Ubiquitin-activating enzyme E1 four-helix bundle
ThiF	PF00899	475 - 967	6e-59	ThiF family
UBA_E1_SCCH	PF10585	662 - 915	2e-84	Ubiquitin-activating enzyme, SCCH domain
E1_UFD	PF09358	986 - 1075	1.2e-23	Ubiquitin fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30110 AT5G06460
fragaria_vesca	FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_6g10930 FvH4_6g10930 FvH4_6g10930
malus_domestica	MD04G1163500.v1.1 MD11G1065700.v1.1 MD12G1177300.v1.1
prunus_persica	Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G282900_v2.0.a1
pyrus_communis	pycom04g14620 pycom11g05570 pycom12g16790
rosa_chinensis	RchiOBHm_Chr3g0462201 RchiOBHm_Chr5g0070911
rosa_laevigata	RLG00000024878 RLG00000036170
rosa_multiflora	Rmu_sc0002944.1_g000004 Rmu_sc0005970.1_g000009 Rmu_sc0006715.1_g000003
rosa_roxburghii	Rroxscaffold_1G00010290 Rroxscaffold_6G00417690
rosa_rugosa	Rorug03G0054900 Rorug05G0408500 Rorug05G0408500 Rorug05G0408500
rosa_samantha	Rh3CG119500 Rh5AG464600 Rh5DG494900
rosa_wichuraiana	Rw3G009590 Rw5G043220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1688
AasI	GACNNNNNNGTC	2 cut(s)	2558, 3206
AatII	GACGTC	1 cut(s)	3210
AccB1I	GGYRCC	2 cut(s)	320, 1928
AccI	GTMKAC	1 cut(s)	780
AccII	CGCG	1 cut(s)	1733
AciI	CCGC	6 cut(s)	695, 1284, 1733, 1985, 2052, 3149
AclWI	GGATC	7 cut(s)	486, 540, 1007, 1020, 2401, 2414, 3123
AcoI	YGGCCR	1 cut(s)	887
AcsI	RAATTY	5 cut(s)	590, 689, 982, 1325, 1528
AcuI	CTGAAG	6 cut(s)	1125, 2076, 2286, 2602, 2742, 3220
AcyI	GRCGYC	2 cut(s)	274, 3207
AfaI	GTAC	7 cut(s)	883, 1879, 1969, 2090, 2284, 2336, 2822
AfeI	AGCGCT	1 cut(s)	1005
AfiI	CCNNNNNNNGG	7 cut(s)	728, 910, 1127, 2164, 2697, 2725, 2941
AflII	CTTAAG	1 cut(s)	1007
AgsI	TTSAA	9 cut(s)	803, 853, 988, 1472, 1525, 1702, 2162, 2784, 3067
AhlI	ACTAGT	1 cut(s)	2279
AjnI	CCWGG	6 cut(s)	546, 759, 1015, 1080, 2198, 2877
AleI	CACNNNNGTG	1 cut(s)	959
Alw21I	GWGCWC	2 cut(s)	1507, 2293
Alw26I	GTCTC	8 cut(s)	257, 281, 505, 1730, 2171, 2360, 2390, 3229
AlwI	GGATC	7 cut(s)	486, 540, 1007, 1020, 2401, 2414, 3123
AlwNI	CAGNNNCTG	4 cut(s)	1667, 2378, 2453, 3010
Ama87I	CYCGRG	4 cut(s)	301, 1393, 2813, 3123
Aor51HI	AGCGCT	1 cut(s)	1005
AoxI	GGCC	6 cut(s)	683, 887, 1055, 1449, 1710, 1956
ApeKI	GCWGC	7 cut(s)	1271, 1667, 1676, 1679, 2273, 2444, 2555
ApoI	RAATTY	5 cut(s)	590, 689, 982, 1325, 1528
ArsI	GACNNNNNNTTYG	2 cut(s)	176, 208
Asp700I	GAANNNNTTC	1 cut(s)	2910
AspLEI	GCGC	3 cut(s)	1006, 1733, 3032
AspS9I	GGNCC	6 cut(s)	683, 1449, 1711, 1939, 1957, 2827
AsuC2I	CCSGG	1 cut(s)	1183
AsuHPI	GGTGA	5 cut(s)	821, 892, 1202, 2126, 2938
AsuII	TTCGAA	2 cut(s)	203, 2640
AvaI	CYCGRG	4 cut(s)	301, 1393, 2813, 3123
AvaII	GGWCC	2 cut(s)	1939, 2827
BaeGI	GKGCMC	1 cut(s)	2206
BaeI	ACNNNNGTAYC	2 cut(s)	2405, 2438
BamHI	GGATCC	2 cut(s)	1012, 2406
BanI	GGYRCC	2 cut(s)	320, 1928
BanII	GRGCYC	2 cut(s)	2017, 2293
BbsI	GAAGAC	3 cut(s)	111, 2051, 3050
Bbv12I	GWGCWC	2 cut(s)	1507, 2293
BbvI	GCAGC	7 cut(s)	1258, 1654, 1663, 1666, 2260, 2456, 2542
BceAI	ACGGC	4 cut(s)	69, 175, 233, 902
BcgI	CGANNNNNNTGC	2 cut(s)	2558, 2592
BciT130I	CCWGG	6 cut(s)	548, 761, 1017, 1082, 2200, 2879
BclI	TGATCA	3 cut(s)	1090, 1213, 1996
BcnI	CCSGG	1 cut(s)	1183
BcoDI	GTCTC	8 cut(s)	257, 281, 505, 1730, 2171, 2360, 2390, 3229
BcuI	ACTAGT	1 cut(s)	2279
BfaI	CTAG	6 cut(s)	18, 504, 815, 2076, 2280, 2687
BfmI	CTRYAG	1 cut(s)	2556
BfoI	RGCGCY	1 cut(s)	1007
BfrI	CTTAAG	1 cut(s)	1007
BglI	GCCNNNNNGGC	1 cut(s)	2879
BglII	AGATCT	1 cut(s)	226
BisI	GCNGC	7 cut(s)	1272, 1668, 1677, 1680, 2274, 2445, 2556
BlpI	GCTNAGC	1 cut(s)	3026
BlsI	GCNGC	7 cut(s)	1273, 1669, 1678, 1681, 2275, 2446, 2557
BmcAI	AGTACT	1 cut(s)	2336
Bme1390I	CCNGG	7 cut(s)	548, 761, 1017, 1082, 1183, 2200, 2879
Bme18I	GGWCC	2 cut(s)	1939, 2827
BmeT110I	CYCGRG	4 cut(s)	301, 1393, 2813, 3123
BmgT120I	GGNCC	6 cut(s)	683, 1449, 1711, 1939, 1957, 2827
BmrFI	CCNGG	7 cut(s)	548, 761, 1017, 1082, 1183, 2200, 2879
BmrI	ACTGGG	2 cut(s)	2075, 2808
BmuI	ACTGGG	2 cut(s)	2075, 2808
BoxI	GACNNNNGTC	2 cut(s)	603, 2352
BpiI	GAAGAC	3 cut(s)	111, 2051, 3050
BpmI	CTGGAG	3 cut(s)	2060, 2315, 3032
Bpu10I	CCTNAGC	1 cut(s)	2121
Bpu1102I	GCTNAGC	1 cut(s)	3026
Bpu14I	TTCGAA	2 cut(s)	203, 2640
BpuEI	CTTGAG	5 cut(s)	815, 942, 1880, 1918, 2957
BpuMI	CCSGG	1 cut(s)	1183
Bsa29I	ATCGAT	2 cut(s)	90, 1160
BsaBI	GATNNNNATC	1 cut(s)	225
BsaHI	GRCGYC	2 cut(s)	274, 3207
BsaI	GGTCTC	1 cut(s)	2171
BsaJI	CCNNGG	8 cut(s)	866, 1016, 1248, 1561, 2199, 2486, 2790, 2898
BsaWI	WCCGGW	1 cut(s)	2285
BsaXI	ACNNNNNCTCC	2 cut(s)	2275, 2305
Bsc4I	CCNNNNNNNGG	7 cut(s)	728, 910, 1127, 2164, 2697, 2725, 2941
Bse118I	RCCGGY	1 cut(s)	1419
Bse1I	ACTGG	7 cut(s)	1342, 1640, 1800, 2081, 2332, 2590, 2803
Bse3DI	GCAATG	4 cut(s)	735, 1281, 2574, 2772
Bse8I	GATNNNNATC	1 cut(s)	225
BseBI	CCWGG	6 cut(s)	548, 761, 1017, 1082, 2200, 2879
BseCI	ATCGAT	2 cut(s)	90, 1160
BseDI	CCNNGG	8 cut(s)	866, 1016, 1248, 1561, 2199, 2486, 2790, 2898
BseJI	GATNNNNATC	1 cut(s)	225
BseLI	CCNNNNNNNGG	7 cut(s)	728, 910, 1127, 2164, 2697, 2725, 2941
BseMI	GCAATG	4 cut(s)	735, 1281, 2574, 2772
BseMII	CTCAG	9 cut(s)	840, 1146, 1160, 1624, 1731, 2013, 2135, 2201, 2409
BseNI	ACTGG	7 cut(s)	1342, 1640, 1800, 2081, 2332, 2590, 2803
BseSI	GKGCMC	1 cut(s)	2206
BseXI	GCAGC	7 cut(s)	1258, 1654, 1663, 1666, 2260, 2456, 2542
BseYI	CCCAGC	1 cut(s)	2767
BsgI	GTGCAG	1 cut(s)	215
Bsh1236I	CGCG	1 cut(s)	1733
BshFI	GGCC	6 cut(s)	685, 889, 1057, 1451, 1712, 1958
BshNI	GGYRCC	2 cut(s)	320, 1928
BshVI	ATCGAT	2 cut(s)	90, 1160
BsiHKAI	GWGCWC	2 cut(s)	1507, 2293
BsiHKCI	CYCGRG	4 cut(s)	301, 1393, 2813, 3123
BsiSI	CCGG	3 cut(s)	1182, 1420, 2286
BslFI	GGGAC	5 cut(s)	413, 1952, 2144, 2456, 2972
BslI	CCNNNNNNNGG	7 cut(s)	728, 910, 1127, 2164, 2697, 2725, 2941
BsmAI	GTCTC	8 cut(s)	257, 281, 505, 1730, 2171, 2360, 2390, 3229
BsmBI	CGTCTC	2 cut(s)	281, 3229
BsmFI	GGGAC	5 cut(s)	413, 1952, 2144, 2456, 2972
BsmI	GAATGC	3 cut(s)	1076, 1975, 2068
BsnI	GGCC	6 cut(s)	685, 889, 1057, 1451, 1712, 1958
Bso31I	GGTCTC	1 cut(s)	2171
BsoBI	CYCGRG	4 cut(s)	301, 1393, 2813, 3123
Bsp119I	TTCGAA	2 cut(s)	203, 2640
Bsp1286I	GDGCHC	4 cut(s)	1507, 2017, 2206, 2293
Bsp1407I	TGTACA	2 cut(s)	1967, 2820
Bsp1720I	GCTNAGC	1 cut(s)	3026
Bsp19I	CCATGG	2 cut(s)	2790, 2898
BspACI	CCGC	6 cut(s)	695, 1284, 1733, 1985, 2052, 3149
BspANI	GGCC	6 cut(s)	685, 889, 1057, 1451, 1712, 1958
BspCNI	CTCAG	9 cut(s)	839, 1145, 1159, 1623, 1732, 2014, 2134, 2202, 2410
BspDI	ATCGAT	2 cut(s)	90, 1160
BspFNI	CGCG	1 cut(s)	1733
BspMAI	CTGCAG	1 cut(s)	2560
BspPI	GGATC	7 cut(s)	486, 540, 1007, 1020, 2401, 2414, 3123
BspT104I	TTCGAA	2 cut(s)	203, 2640
BspT107I	GGYRCC	2 cut(s)	320, 1928
BspTI	CTTAAG	1 cut(s)	1007
BspTNI	GGTCTC	1 cut(s)	2171
BsrDI	GCAATG	4 cut(s)	735, 1281, 2574, 2772
BsrFI	RCCGGY	1 cut(s)	1419
BsrGI	TGTACA	2 cut(s)	1967, 2820
BsrI	ACTGG	7 cut(s)	1342, 1640, 1800, 2081, 2332, 2590, 2803
BssAI	RCCGGY	1 cut(s)	1419
BssECI	CCNNGG	8 cut(s)	866, 1016, 1248, 1561, 2199, 2486, 2790, 2898
BssNI	GRCGYC	2 cut(s)	274, 3207
BssT1I	CCWWGG	6 cut(s)	866, 1248, 1561, 2486, 2790, 2898
Bst2UI	CCWGG	6 cut(s)	548, 761, 1017, 1082, 2200, 2879
Bst6I	CTCTTC	4 cut(s)	1131, 1343, 1595, 2994
BstACI	GRCGYC	2 cut(s)	274, 3207
BstAFI	CTTAAG	1 cut(s)	1007
BstAUI	TGTACA	2 cut(s)	1967, 2820
BstBI	TTCGAA	2 cut(s)	203, 2640
BstC8I	GCNNGC	7 cut(s)	15, 242, 246, 1111, 1129, 1315, 2017
BstDSI	CCRYGG	2 cut(s)	2790, 2898
BstEII	GGTNACC	1 cut(s)	809
BstENI	CCTNNNNNAGG	2 cut(s)	908, 2162
BstFNI	CGCG	1 cut(s)	1733
BstH2I	RGCGCY	1 cut(s)	1007
BstHHI	GCGC	3 cut(s)	1006, 1733, 3032
BstMAI	GTCTC	8 cut(s)	257, 281, 505, 1730, 2171, 2360, 2390, 3229
BstNI	CCWGG	6 cut(s)	548, 761, 1017, 1082, 2200, 2879
BstPAI	GACNNNNGTC	2 cut(s)	603, 2352
BstPI	GGTNACC	1 cut(s)	809
BstSCI	CCNGG	7 cut(s)	546, 759, 1015, 1080, 1181, 2198, 2877
BstSFI	CTRYAG	1 cut(s)	2556
BstSLI	GKGCMC	1 cut(s)	2206
BstUI	CGCG	1 cut(s)	1733
BstV1I	GCAGC	7 cut(s)	1258, 1654, 1663, 1666, 2260, 2456, 2542
BstV2I	GAAGAC	3 cut(s)	111, 2051, 3050
BstX2I	RGATCY	6 cut(s)	226, 478, 532, 1012, 2406, 3115
BstYI	RGATCY	6 cut(s)	226, 478, 532, 1012, 2406, 3115
Bsu15I	ATCGAT	2 cut(s)	90, 1160
BsuRI	GGCC	6 cut(s)	685, 889, 1057, 1451, 1712, 1958
BsuTUI	ATCGAT	2 cut(s)	90, 1160
BtgI	CCRYGG	2 cut(s)	2790, 2898
BtsI	GCAGTG	2 cut(s)	2535, 3011
BtsIMutI	CAGTG	9 cut(s)	96, 1368, 1581, 1793, 1977, 1999, 2535, 2597, 3011
Cac8I	GCNNGC	7 cut(s)	15, 242, 246, 1111, 1129, 1315, 2017
CaiI	CAGNNNCTG	4 cut(s)	1667, 2378, 2453, 3010
CfoI	GCGC	3 cut(s)	1006, 1733, 3032
Cfr10I	RCCGGY	1 cut(s)	1419
Cfr13I	GGNCC	6 cut(s)	683, 1449, 1711, 1939, 1957, 2827
ClaI	ATCGAT	2 cut(s)	90, 1160
CseI	GACGC	5 cut(s)	157, 263, 729, 1151, 2561
Csp6I	GTAC	7 cut(s)	882, 1878, 1968, 2089, 2283, 2335, 2821
CviAII	CATG	9 cut(s)	187, 374, 574, 1963, 2668, 2695, 2791, 2899, 2941
CviQI	GTAC	7 cut(s)	882, 1878, 1968, 2089, 2283, 2335, 2821
DraI	TTTAAA	2 cut(s)	1099, 1197
DrdI	GACNNNNNNGTC	2 cut(s)	2558, 3206
DseDI	GACNNNNNNGTC	2 cut(s)	2558, 3206
EaeI	YGGCCR	1 cut(s)	887
Eam1104I	CTCTTC	4 cut(s)	1131, 1343, 1595, 2994
EarI	CTCTTC	4 cut(s)	1131, 1343, 1595, 2994
Ecl136II	GAGCTC	1 cut(s)	2291
Eco130I	CCWWGG	6 cut(s)	866, 1248, 1561, 2486, 2790, 2898
Eco24I	GRGCYC	2 cut(s)	2017, 2293
Eco31I	GGTCTC	1 cut(s)	2171
Eco32I	GATATC	1 cut(s)	1591
Eco47I	GGWCC	2 cut(s)	1939, 2827
Eco47III	AGCGCT	1 cut(s)	1005
Eco53kI	GAGCTC	1 cut(s)	2291
Eco57I	CTGAAG	6 cut(s)	1125, 2076, 2286, 2602, 2742, 3220
Eco88I	CYCGRG	4 cut(s)	301, 1393, 2813, 3123
Eco91I	GGTNACC	1 cut(s)	809
EcoICRI	GAGCTC	1 cut(s)	2291
EcoNI	CCTNNNNNAGG	2 cut(s)	908, 2162
EcoO109I	RGGNCCY	4 cut(s)	1711, 1939, 1957, 2827
EcoO65I	GGTNACC	1 cut(s)	809
EcoRII	CCWGG	6 cut(s)	546, 759, 1015, 1080, 2198, 2877
EcoRV	GATATC	1 cut(s)	1591
EcoT14I	CCWWGG	6 cut(s)	866, 1248, 1561, 2486, 2790, 2898
EcoT22I	ATGCAT	1 cut(s)	2068
EcoT38I	GRGCYC	2 cut(s)	2017, 2293
ErhI	CCWWGG	6 cut(s)	866, 1248, 1561, 2486, 2790, 2898
Esp3I	CGTCTC	2 cut(s)	281, 3229
FaeI	CATG	9 cut(s)	190, 377, 577, 1966, 2671, 2698, 2794, 2902, 2944
FalI	AAGNNNNNCTT	2 cut(s)	519, 551
FaqI	GGGAC	5 cut(s)	413, 1952, 2144, 2456, 2972
FatI	CATG	9 cut(s)	186, 373, 573, 1962, 2667, 2694, 2790, 2898, 2940
FauI	CCCGC	2 cut(s)	1992, 2059
FauNDI	CATATG	1 cut(s)	934
FbaI	TGATCA	3 cut(s)	1090, 1213, 1996
FblI	GTMKAC	1 cut(s)	780
Fnu4HI	GCNGC	7 cut(s)	1272, 1668, 1677, 1680, 2274, 2445, 2556
FriOI	GRGCYC	2 cut(s)	2017, 2293
Fsp4HI	GCNGC	7 cut(s)	1272, 1668, 1677, 1680, 2274, 2445, 2556
FspBI	CTAG	6 cut(s)	18, 504, 815, 2076, 2280, 2687
GlaI	GCGC	3 cut(s)	1005, 1732, 3031
GluI	GCNGC	7 cut(s)	1272, 1668, 1677, 1680, 2274, 2445, 2556
GsaI	CCCAGC	1 cut(s)	2771
GsuI	CTGGAG	3 cut(s)	2060, 2315, 3032
HaeII	RGCGCY	1 cut(s)	1007
HaeIII	GGCC	6 cut(s)	685, 889, 1057, 1451, 1712, 1958
HapII	CCGG	3 cut(s)	1182, 1420, 2286
HgaI	GACGC	5 cut(s)	157, 263, 729, 1151, 2561
HhaI	GCGC	3 cut(s)	1006, 1733, 3032
Hin1I	GRCGYC	2 cut(s)	274, 3207
Hin1II	CATG	9 cut(s)	190, 377, 577, 1966, 2671, 2698, 2794, 2902, 2944
Hin6I	GCGC	3 cut(s)	1004, 1731, 3030
HinP1I	GCGC	3 cut(s)	1004, 1731, 3030
HincII	GTYRAC	2 cut(s)	781, 2458
HindII	GTYRAC	2 cut(s)	781, 2458
HindIII	AAGCTT	5 cut(s)	517, 1151, 1220, 1311, 2853
HpaII	CCGG	3 cut(s)	1182, 1420, 2286
HphI	GGTGA	5 cut(s)	821, 892, 1202, 2126, 2938
Hpy99I	CGWCG	6 cut(s)	173, 785, 2344, 2555, 2570, 3212
HpyAV	CCTTC	6 cut(s)	934, 1076, 1771, 1990, 2191, 3100
HpyCH4IV	ACGT	1 cut(s)	3207
HpySE526I	ACGT	1 cut(s)	3207
Hsp92I	GRCGYC	2 cut(s)	274, 3207
Hsp92II	CATG	9 cut(s)	190, 377, 577, 1966, 2671, 2698, 2794, 2902, 2944
HspAI	GCGC	3 cut(s)	1004, 1731, 3030
KflI	GGGWCCC	1 cut(s)	1939
Ksp22I	TGATCA	3 cut(s)	1090, 1213, 1996
LmnI	GCTCC	4 cut(s)	1244, 2288, 2296, 2311
Lsp1109I	GCAGC	7 cut(s)	1258, 1654, 1663, 1666, 2260, 2456, 2542
MaeI	CTAG	6 cut(s)	18, 504, 815, 2076, 2280, 2687
MaeII	ACGT	1 cut(s)	3207
MfeI	CAATTG	1 cut(s)	2246
MflI	RGATCY	6 cut(s)	226, 478, 532, 1012, 2406, 3115
MhlI	GDGCHC	4 cut(s)	1507, 2017, 2206, 2293
MlyI	GAGTC	4 cut(s)	1376, 1385, 1871, 2041
MmeI	TCCRAC	2 cut(s)	1273, 2370
Mph1103I	ATGCAT	1 cut(s)	2068
MroXI	GAANNNNTTC	1 cut(s)	2910
MslI	CAYNNNNRTG	5 cut(s)	572, 959, 2670, 2672, 3168
MspA1I	CMGCKG	1 cut(s)	2054
MspCI	CTTAAG	1 cut(s)	1007
MspI	CCGG	3 cut(s)	1182, 1420, 2286
MspR9I	CCNGG	7 cut(s)	548, 761, 1017, 1082, 1183, 2200, 2879
MunI	CAATTG	1 cut(s)	2246
Mva1269I	GAATGC	3 cut(s)	1076, 1975, 2068
MvaI	CCWGG	6 cut(s)	548, 761, 1017, 1082, 2200, 2879
MvnI	CGCG	1 cut(s)	1733
NciI	CCSGG	1 cut(s)	1183
NcoI	CCATGG	2 cut(s)	2790, 2898
NdeI	CATATG	1 cut(s)	934
NlaIII	CATG	9 cut(s)	190, 377, 577, 1966, 2671, 2698, 2794, 2902, 2944
NmeAIII	GCCGAG	1 cut(s)	349
NsiI	ATGCAT	1 cut(s)	2068
NspV	TTCGAA	2 cut(s)	203, 2640
OliI	CACNNNNGTG	1 cut(s)	959
PaeR7I	CTCGAG	2 cut(s)	1393, 2813
PcsI	WCGNNNNNNNCGW	2 cut(s)	36, 165
PctI	GAATGC	3 cut(s)	1076, 1975, 2068
PdmI	GAANNNNTTC	1 cut(s)	2910
PfeI	GAWTC	7 cut(s)	101, 337, 1261, 1358, 1826, 2316, 2760
PkrI	GCNGC	7 cut(s)	1273, 1669, 1678, 1681, 2275, 2446, 2557
PleI	GAGTC	4 cut(s)	1375, 1385, 1870, 2041
PpsI	GAGTC	4 cut(s)	1375, 1385, 1870, 2041
PpuMI	RGGWCCY	2 cut(s)	1939, 2827
PshAI	GACNNNNGTC	2 cut(s)	603, 2352
PsiI	TTATAA	1 cut(s)	1688
Psp124BI	GAGCTC	1 cut(s)	2293
Psp5II	RGGWCCY	2 cut(s)	1939, 2827
Psp6I	CCWGG	6 cut(s)	546, 759, 1015, 1080, 2198, 2877
PspEI	GGTNACC	1 cut(s)	809
PspFI	CCCAGC	1 cut(s)	2767
PspGI	CCWGG	6 cut(s)	546, 759, 1015, 1080, 2198, 2877
PspPI	GGNCC	6 cut(s)	683, 1449, 1711, 1939, 1957, 2827
PspPPI	RGGWCCY	2 cut(s)	1939, 2827
PspXI	VCTCGAGB	2 cut(s)	1393, 2813
PsrI	GAACNNNNNNTAC	2 cut(s)	1861, 1893
PstI	CTGCAG	1 cut(s)	2560
PstNI	CAGNNNCTG	4 cut(s)	1667, 2378, 2453, 3010
PsuI	RGATCY	6 cut(s)	226, 478, 532, 1012, 2406, 3115
RsaI	GTAC	7 cut(s)	883, 1879, 1969, 2090, 2284, 2336, 2822
RsaNI	GTAC	7 cut(s)	882, 1878, 1968, 2089, 2283, 2335, 2821
RseI	CAYNNNNRTG	5 cut(s)	572, 959, 2670, 2672, 3168
SacI	GAGCTC	1 cut(s)	2293
SalI	GTCGAC	1 cut(s)	779
SatI	GCNGC	7 cut(s)	1272, 1668, 1677, 1680, 2274, 2445, 2556
Sau96I	GGNCC	6 cut(s)	683, 1449, 1711, 1939, 1957, 2827
ScaI	AGTACT	1 cut(s)	2336
SchI	GAGTC	4 cut(s)	1376, 1385, 1871, 2041
ScrFI	CCNGG	7 cut(s)	548, 761, 1017, 1082, 1183, 2200, 2879
SduI	GDGCHC	4 cut(s)	1507, 2017, 2206, 2293
SfcI	CTRYAG	1 cut(s)	2556
Sfr274I	CTCGAG	2 cut(s)	1393, 2813
SfuI	TTCGAA	2 cut(s)	203, 2640
SinI	GGWCC	2 cut(s)	1939, 2827
SlaI	CTCGAG	2 cut(s)	1393, 2813
SmiMI	CAYNNNNRTG	5 cut(s)	572, 959, 2670, 2672, 3168
SmlI	CTYRAG	8 cut(s)	794, 957, 1007, 1393, 1859, 1933, 2813, 2972
SmoI	CTYRAG	8 cut(s)	794, 957, 1007, 1393, 1859, 1933, 2813, 2972
SpeI	ACTAGT	1 cut(s)	2279
SsiI	CCGC	6 cut(s)	695, 1284, 1733, 1985, 2052, 3149
SspMI	CTAG	6 cut(s)	18, 504, 815, 2076, 2280, 2687
SstI	GAGCTC	1 cut(s)	2293
StyD4I	CCNGG	7 cut(s)	546, 759, 1015, 1080, 1181, 2198, 2877
StyI	CCWWGG	6 cut(s)	866, 1248, 1561, 2486, 2790, 2898
TaiI	ACGT	1 cut(s)	3210
TatI	WGTACW	5 cut(s)	881, 1967, 2088, 2334, 2820
TfiI	GAWTC	7 cut(s)	101, 337, 1261, 1358, 1826, 2316, 2760
TscAI	CASTG	9 cut(s)	103, 1368, 1581, 1800, 1977, 2006, 2542, 2597, 3011
TseI	GCWGC	7 cut(s)	1271, 1667, 1676, 1679, 2273, 2444, 2555
TspDTI	ATGAA	7 cut(s)	237, 393, 885, 1319, 2639, 2737, 3207
TspGWI	ACGGA	1 cut(s)	1619
TspRI	CASTG	9 cut(s)	103, 1368, 1581, 1800, 1977, 2006, 2542, 2597, 3011
Vha464I	CTTAAG	1 cut(s)	1007
VpaK11BI	GGWCC	2 cut(s)	1939, 2827
XagI	CCTNNNNNAGG	2 cut(s)	908, 2162
XapI	RAATTY	5 cut(s)	590, 689, 982, 1325, 1528
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2542
XhoI	CTCGAG	2 cut(s)	1393, 2813
XmiI	GTMKAC	1 cut(s)	780
XmnI	GAANNNNTTC	1 cut(s)	2910
XspI	CTAG	6 cut(s)	18, 504, 815, 2076, 2280, 2687
ZraI	GACGTC	1 cut(s)	3208
ZrmI	AGTACT	1 cut(s)	2336
Zsp2I	ATGCAT	1 cut(s)	2068

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.