Rw3G009590

Ubiquitin-activating enzyme E1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002217
Physical Location & Seq
Forward (+)
9177681 .. 9182995
5315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G009590.1

Sequence Viewer

Length: 3273 bp
ATGGAGTTGTGGTTGGGCAGCTCATCGCACTATATGCTTCCTAGAAAGAGACAAGTGGGCGGAGAGGTTGTAGTAGAAGAAGGGGAACCAGATTTCGCCAACGAATCTCCGATCAAGAAGCTTTGCACCGACGAATCTAAGGGCAGTGACTGCAAAAACACTGGTAACAGCAATATCAGCAGCAGCAACAGTAGCAGCAGCGTCAAGCCGCCGATCATGGCATTGGGAAATGGGAATTCGGGGGATATTGATGAGGATCTCCACAGCCGGCAGCTTGCTGTGTACGGCCGTGAGACGATGAGGCGGCTCTTTGCCTCCAACATTCTTGTCTCTGGGATGCAAGGCCTTGGTGCCGAGATTGCAAAGAACCTTGTTCTTGCCGGTGTCAAGTCTGTGACCTTGCATGATGAAGGAGTTGTTGAGTTGTGGGACCTGTCTGGTAATTTCTTTTTCACTGAAGACGATATTGGAAAGAATCGGGCCCTTGCTTGTGTCCAAAAATTGCAAGAACTTAATAATGCTGTGCTCATTTCGACTTTGACAACTCAGCTGACTAAAGAAAAACTTTCTGATTTCCAGGCTGTAGTTTTCACTGATATCAGCTTAGACAAAGCAATTGAATTCGATGATTACTGCCATAATCATCAGCCTCCAATCTCTTTTATCAAAACTGAAGTACGGGGCCTTTTTGGCAGTGTGTTTTGTGACTTTGGTCCTGAGTTCACTGTTCTTGATGTTGATGGTGAGGATCCACACACAGGCATAGTCGCATCAATTAGCAATGACAACCCTGCTATGATATCATGTGTCGATGATGAGAGGCTTGAGTTTCAGGACGGGGATTTGGTTCTGTTCACTGAAGTCCATGGAATGACCGAGTTGAATGACGGAAAGCCGAGGAAGATTAAGAATGCAAGGCCGTATTCATTCACAATTGAGCAGGACACTACAAATTATGCAGGATATGAGAAAGGTGGTATAGTTACACAGGTTAAGCAACCCAAGGTGTTGAAATTTAAGCCCTTGCGAGAAGCAGTCAAGGAACCTGGTGATTTCCTTCTGATTGACTTCTCCAAGTTTGATCGGCCACCTCTCCTGCACTTGGCATTTCAGGCACTAGATAAGTTTATATCAGAGTTAGGACGTTTCCCTGTTGCTGGATCCGAGGATGATGCTACAAAGTTCATATCATTGGTAACTAGCATAAATGATGGCTCGGCAGATGGGAGGCTTGAGGAGATTGATCAGAAGATTCTTCGTCACTTTGCATTTGGTGCTAGGGCTGTGCTGAATCCCATGGCTGCAATGTTTGGTGGTATTGTTGGACAGGAGGTTGTGAAGGCCTGTTCTTCAAAGTTTCACCCTCTTTTCCAGTTTTTCTATTTCGATTCGGTTGAATCACTTCCGACAGAAGCCTTCGACCCTAGTGATTTGAAGCCTCTAAACAGTCGGTATGATGCACAAATTTCAGTATTTGGAGCCAAGCTGCAGAAAAAGCTGGAGGATTCAAAAGTGTTCACTGTTGGGTCTGGTGCACTAGGATGCGAGTTTTTGAAAAATTTAGCTTTGATGGGCGTTGCTTGTGGTCAAGAAGGTAAATTAACAATTACAGATGATGATGTCATTGAGAAGAGTAACCTTAGTAGACAATTTCTCTTCCGGGATTGGAACATTGGGCAGGCTAAATCAACAGTTGCTGCCACTGCTGCTGCACTTATAAACAGTCGTATCAATATTGAAGCACTGCAGAATCGTGCAAGCCCAGAGACTGAAAACGTGTTTGATGATACTTTCTGGGAGAATTTGGATGTTGTTATCAATGCTCTAGATAATGTGAATGCTAGGCTTTACATTGATCAGCGATGCTTGTATTTCCAGAAGCCACTTTTGGAATCAGGTACCCTAGGTGCCAAGTGCAACACACAGATGGTCATTCCTCACTTGACTGAAAATTATGGAGCATCACGGGACCCACCTGAAAAGCAAGCACCTATGTGCACAGTTCATTCATTTCCTCACAACATTGACCACTGCTTGACATGGGCTCGTTCTGAGTTTGAGGGTTTACTTGAGAAGGTCCCAGCTGAAGTAAATGCCTACTTGACAAATCCTAGTGAGTACACTACTGCAATGAAGAATGCTGGTGATGCTCAGGCAAGGAATAACTTGGAAAGTGTCATTGAATGCCTTGACAAGGAGAGATGCGAGACATTTCAAGATTGCATAACCTGGGCCCGACTAAAGTTTGAGGACTACTTTTCCAACCGTGTGAAGCAGTTAACATATACTTTTCCTGAGGATGCTACAACCAGTAGTGGGACCCCATTCTGGTCTGCTCCCAAGCGTTTCCCTCGCCCACTGGTGTTCTCAGTAGATGATCTCAGCCACCTTCAATTTGTACTGGCAGCATCCATACTACGAGCTGAGACATTTAACATTGCAATTCCTGATTGGATCAAGTCTACTCAAAAGTTTGCTGATGCTGTTAACAACGTGATGGTACCTGAATTTCAGCCTAAGAAGGATGTGAAGATTGTAATAGATGAGAAAGCTACAAACATTCTACCGGCATCCATCGATGATGCTGCTGTTATCAACGAGTTAGTTATGAAGTTAGAAAAATGCAGGGAGCAGCTCCCACCAGCCTTCAAGATGAACCCTATTCAATTTGAGAAGGATGATGACACAAACTATCATATGGATGTAATAGCTGGATTTGCAAATATGAGGGCTAGGAATTATAGTATCCCTGAAGTCGACAAGCTGAAGGCCAAATTCATCGCCGGTAGAATCATTCCTGCAATTGCAACGTCTACTGCTCTTGCAACTGGTCTTGTCTGCTTGGAGCTGTACAAGGTTCTGGCCGGAGAGCACAAGATAGAGGACTACCGAAACACCTTTGCCAATCTTGCTCTTCCTCTGTTCTCCATGGCTGAACCAGTACCTCCTAAAGTCATCAAGCACCAAGATATGAGCTGGACAGTTTGGGACAGATGGATTATAAAGGACAACCCATCTTTGAAGCAGCTCCTTAATTGGCTCAAAGAAAAGGGCTTAAACGCTTACAGCATCTCTTATGGCAGTTGCCTTCTGTATAACAGCATGTTCCCCAAGCACAGAGAGCGCATGGACAAGCATATGGTGGATTTGGCTAGGGATGTGGCAAAGGCGGAGCTGCCTCCATATCGGAACCACTTTGATGTGGTGGTGGCCTGCGAAGATGATGAGGATAATGATATTGACATCCCTCAGATCTCCATTTACTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko05012 map04120 map05012
Pfam Domains
Protein Families
E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1090

Amino Acids

121.87

Weight (kDa)

5.07

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 88 - 463 1.4e-24 ThiF family
E1_FCCH PF16190 262 - 332 4.9e-28 Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB PF16191 333 - 403 1.2e-24 Ubiquitin-activating enzyme E1 four-helix bundle
ThiF PF00899 485 - 977 4.3e-61 ThiF family
UBA_E1_SCCH PF10585 672 - 925 1.8e-86 Ubiquitin-activating enzyme, SCCH domain
E1_UFD PF09358 996 - 1085 4.5e-24 Ubiquitin fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1718, 3002
Acc65I GGTACC 2 cut(s) 1898, 2500
AccB1I GGYRCC 4 cut(s) 350, 1898, 1907, 2500
AccI GTMKAC 4 cut(s) 1645, 2462, 2757, 2813
AciI CCGC 4 cut(s) 60, 209, 304, 3170
AclWI GGATC 6 cut(s) 264, 743, 756, 1155, 1168, 2462
AcoI YGGCCR 3 cut(s) 286, 1085, 2862
AcsI RAATTY 8 cut(s) 235, 620, 1013, 1464, 1558, 1801, 2507, 2774
AcuI CTGAAG 6 cut(s) 477, 693, 879, 2106, 2772, 2786
AfaI GTAC 8 cut(s) 284, 678, 1900, 2120, 2400, 2502, 2852, 2943
AfiI CCNNNNNNNGG 6 cut(s) 758, 1102, 1157, 2194, 2316, 2328
AflIII ACRYGT 1 cut(s) 1776
AjnI CCWGG 3 cut(s) 576, 1045, 2228
AjuI GAANNNNNNNTTGG 4 cut(s) 450, 482, 1871, 1903
AleI CACNNNNGTG 1 cut(s) 1993
Alw21I GWGCWC 4 cut(s) 528, 1537, 2000, 2874
Alw26I GTCTC 6 cut(s) 43, 287, 334, 1760, 2201, 2420
Alw44I GTGCAC 2 cut(s) 1533, 1996
AlwI GGATC 6 cut(s) 264, 743, 756, 1155, 1168, 2462
AlwNI CAGNNNCTG 3 cut(s) 150, 1697, 1769
ApaI GGGCCC 2 cut(s) 484, 2236
ApaLI GTGCAC 2 cut(s) 1533, 1996
ApoI RAATTY 8 cut(s) 235, 620, 1013, 1464, 1558, 1801, 2507, 2774
Asp700I GAANNNNTTC 1 cut(s) 1401
Asp718I GGTACC 2 cut(s) 1898, 2500
AspA2I CCTAGG 1 cut(s) 1903
AspLEI GCGC 1 cut(s) 3126
AsuC2I CCSGG 1 cut(s) 1661
AsuHPI GGTGA 4 cut(s) 755, 1061, 1352, 2156
AvaII GGWCC 5 cut(s) 430, 713, 1969, 2077, 2319
AvrII CCTAGG 1 cut(s) 1903
AxyI CCTNAGG 1 cut(s) 2295
BaeGI GKGCMC 4 cut(s) 484, 1537, 2000, 2236
BaeI ACNNNNGTAYC 2 cut(s) 1711, 1744
BamHI GGATCC 2 cut(s) 748, 1160
BanI GGYRCC 4 cut(s) 350, 1898, 1907, 2500
BanII GRGCYC 3 cut(s) 484, 2047, 2236
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 4 cut(s) 528, 1537, 2000, 2874
BccI CCATC 9 cut(s) 734, 1205, 1217, 1564, 1921, 2491, 2582, 2988, 3022
BceAI ACGGC 3 cut(s) 273, 301, 904
BcgI CGANNNNNNTGC 4 cut(s) 1154, 1188, 2567, 2601
BciT130I CCWGG 3 cut(s) 578, 1047, 2230
BciVI GTATCC 1 cut(s) 2756
BclI TGATCA 2 cut(s) 1243, 1855
BcnI CCSGG 1 cut(s) 1661
BcoDI GTCTC 6 cut(s) 43, 287, 334, 1760, 2201, 2420
BfmI CTRYAG 3 cut(s) 582, 1487, 1745
BfuI GTATCC 1 cut(s) 2756
BglI GCCNNNNNGGC 1 cut(s) 690
BglII AGATCT 1 cut(s) 3252
BlnI CCTAGG 1 cut(s) 1903
Bme1390I CCNGG 4 cut(s) 578, 1047, 1661, 2230
Bme18I GGWCC 5 cut(s) 430, 713, 1969, 2077, 2319
BmrFI CCNGG 4 cut(s) 578, 1047, 1661, 2230
BoxI GACNNNNGTC 1 cut(s) 711
BpiI GAAGAC 1 cut(s) 465
BpmI CTGGAG 1 cut(s) 1520
Bpu10I CCTNAGC 1 cut(s) 2151
BpuEI CTTGAG 3 cut(s) 845, 1253, 2090
BpuMI CCSGG 1 cut(s) 1661
Bsa29I ATCGAT 1 cut(s) 2577
BsaBI GATNNNNATC 1 cut(s) 255
BsaJI CCNNGG 9 cut(s) 346, 865, 896, 1002, 1164, 1296, 1903, 2229, 2928
BsaXI ACNNNNNCTCC 3 cut(s) 26, 54, 84
Bsc4I CCNNNNNNNGG 6 cut(s) 758, 1102, 1157, 2194, 2316, 2328
Bse118I RCCGGY 4 cut(s) 267, 380, 2566, 2783
Bse1I ACTGG 7 cut(s) 166, 1372, 2310, 2364, 2406, 2833, 2939
Bse21I CCTNAGG 1 cut(s) 2295
Bse3DI GCAATG 4 cut(s) 787, 1311, 2136, 2436
Bse8I GATNNNNATC 1 cut(s) 255
BseBI CCWGG 3 cut(s) 578, 1047, 2230
BseCI ATCGAT 1 cut(s) 2577
BseDI CCNNGG 9 cut(s) 346, 865, 896, 1002, 1164, 1296, 1903, 2229, 2928
BseJI GATNNNNATC 1 cut(s) 255
BseLI CCNNNNNNNGG 6 cut(s) 758, 1102, 1157, 2194, 2316, 2328
BseMI GCAATG 4 cut(s) 787, 1311, 2136, 2436
BseMII CTCAG 9 cut(s) 560, 708, 2043, 2165, 2286, 2382, 2395, 2415, 3263
BseNI ACTGG 7 cut(s) 166, 1372, 2310, 2364, 2406, 2833, 2939
BseRI GAGGAG 1 cut(s) 1250
BseSI GKGCMC 4 cut(s) 484, 1537, 2000, 2236
BseX3I CGGCCG 1 cut(s) 286
BseYI CCCAGC 1 cut(s) 2080
BsgI GTGCAG 2 cut(s) 1082, 1695
Bsh1285I CGRYCG 1 cut(s) 289
BshNI GGYRCC 4 cut(s) 350, 1898, 1907, 2500
BshVI ATCGAT 1 cut(s) 2577
BsiEI CGRYCG 1 cut(s) 289
BsiHKAI GWGCWC 4 cut(s) 528, 1537, 2000, 2874
BsiSI CCGG 6 cut(s) 268, 381, 1660, 2567, 2784, 2865
BslFI GGGAC 5 cut(s) 443, 1982, 2063, 2332, 3002
BslI CCNNNNNNNGG 6 cut(s) 758, 1102, 1157, 2194, 2316, 2328
BsmAI GTCTC 6 cut(s) 43, 287, 334, 1760, 2201, 2420
BsmBI CGTCTC 1 cut(s) 287
BsmFI GGGAC 5 cut(s) 443, 1982, 2063, 2332, 3002
BsmI GAATGC 4 cut(s) 916, 1843, 2143, 2189
Bsp120I GGGCCC 2 cut(s) 480, 2232
Bsp1286I GDGCHC 7 cut(s) 484, 528, 1537, 2000, 2047, 2236, 2874
Bsp1407I TGTACA 1 cut(s) 2850
Bsp19I CCATGG 3 cut(s) 865, 1296, 2928
BspACI CCGC 4 cut(s) 60, 209, 304, 3170
BspCNI CTCAG 9 cut(s) 559, 709, 2044, 2164, 2287, 2381, 2394, 2416, 3262
BspDI ATCGAT 1 cut(s) 2577
BspMAI CTGCAG 2 cut(s) 1491, 1749
BspPI GGATC 6 cut(s) 264, 743, 756, 1155, 1168, 2462
BspQI GCTCTTC 1 cut(s) 2919
BspT107I GGYRCC 4 cut(s) 350, 1898, 1907, 2500
BsrDI GCAATG 4 cut(s) 787, 1311, 2136, 2436
BsrFI RCCGGY 4 cut(s) 267, 380, 2566, 2783
BsrGI TGTACA 1 cut(s) 2850
BsrI ACTGG 7 cut(s) 166, 1372, 2310, 2364, 2406, 2833, 2939
BssAI RCCGGY 4 cut(s) 267, 380, 2566, 2783
BssECI CCNNGG 9 cut(s) 346, 865, 896, 1002, 1164, 1296, 1903, 2229, 2928
BssT1I CCWWGG 6 cut(s) 346, 865, 1002, 1296, 1903, 2928
Bst2UI CCWGG 3 cut(s) 578, 1047, 2230
Bst4CI ACNGT 9 cut(s) 191, 727, 1448, 1522, 1693, 1724, 2002, 2267, 2983
Bst6I CTCTTC 3 cut(s) 1625, 1661, 2919
BstAPI GCANNNNNTGC 3 cut(s) 34, 150, 1274
BstAUI TGTACA 1 cut(s) 2850
BstC8I GCNNGC 6 cut(s) 269, 276, 1680, 1759, 1986, 3214
BstDSI CCRYGG 3 cut(s) 865, 1296, 2928
BstENI CCTNNNNNAGG 1 cut(s) 2192
BstHHI GCGC 1 cut(s) 3126
BstMAI GTCTC 6 cut(s) 43, 287, 334, 1760, 2201, 2420
BstMCI CGRYCG 1 cut(s) 289
BstNI CCWGG 3 cut(s) 578, 1047, 2230
BstNSI RCATGY 1 cut(s) 3106
BstPAI GACNNNNGTC 1 cut(s) 711
BstSCI CCNGG 4 cut(s) 576, 1045, 1659, 2228
BstSFI CTRYAG 3 cut(s) 582, 1487, 1745
BstSLI GKGCMC 4 cut(s) 484, 1537, 2000, 2236
BstV2I GAAGAC 1 cut(s) 465
BstX2I RGATCY 4 cut(s) 256, 748, 1160, 3252
BstYI RGATCY 4 cut(s) 256, 748, 1160, 3252
BstZI CGGCCG 1 cut(s) 286
Bsu15I ATCGAT 1 cut(s) 2577
Bsu36I CCTNAGG 1 cut(s) 2295
BsuI GTATCC 1 cut(s) 2756
BsuTUI ATCGAT 1 cut(s) 2577
BtgI CCRYGG 3 cut(s) 865, 1296, 2928
BtgZI GCGATG 3 cut(s) 9, 1876, 2764
BtsI GCAGTG 5 cut(s) 151, 700, 1701, 1742, 2029
Cac8I GCNNGC 6 cut(s) 269, 276, 1680, 1759, 1986, 3214
CaiI CAGNNNCTG 3 cut(s) 150, 1697, 1769
CfoI GCGC 1 cut(s) 3126
Cfr10I RCCGGY 4 cut(s) 267, 380, 2566, 2783
ClaI ATCGAT 1 cut(s) 2577
CseI GACGC 1 cut(s) 190
CsiI ACCWGGT 1 cut(s) 1045
Csp6I GTAC 8 cut(s) 283, 677, 1899, 2119, 2399, 2501, 2851, 2942
CviAII CATG 9 cut(s) 217, 404, 804, 866, 1297, 2040, 2929, 3103, 3127
CviQI GTAC 8 cut(s) 283, 677, 1899, 2119, 2399, 2501, 2851, 2942
EaeI YGGCCR 3 cut(s) 286, 1085, 2862
EagI CGGCCG 1 cut(s) 286
Eam1104I CTCTTC 3 cut(s) 1625, 1661, 2919
EarI CTCTTC 3 cut(s) 1625, 1661, 2919
EciI GGCGGA 2 cut(s) 75, 3185
EclXI CGGCCG 1 cut(s) 286
Eco130I CCWWGG 6 cut(s) 346, 865, 1002, 1296, 1903, 2928
Eco147I AGGCCT 2 cut(s) 345, 1343
Eco24I GRGCYC 3 cut(s) 484, 2047, 2236
Eco32I GATATC 2 cut(s) 598, 801
Eco47I GGWCC 5 cut(s) 430, 713, 1969, 2077, 2319
Eco52I CGGCCG 1 cut(s) 286
Eco57I CTGAAG 6 cut(s) 477, 693, 879, 2106, 2772, 2786
Eco81I CCTNAGG 1 cut(s) 2295
EcoNI CCTNNNNNAGG 1 cut(s) 2192
EcoO109I RGGNCCY 6 cut(s) 430, 481, 682, 1969, 2077, 2319
EcoRI GAATTC 2 cut(s) 235, 620
EcoRII CCWGG 3 cut(s) 576, 1045, 2228
EcoRV GATATC 2 cut(s) 598, 801
EcoT14I CCWWGG 6 cut(s) 346, 865, 1002, 1296, 1903, 2928
EcoT38I GRGCYC 3 cut(s) 484, 2047, 2236
ErhI CCWWGG 6 cut(s) 346, 865, 1002, 1296, 1903, 2928
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 9 cut(s) 220, 407, 807, 869, 1300, 2043, 2932, 3106, 3130
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 5 cut(s) 443, 1982, 2063, 2332, 3002
FatI CATG 9 cut(s) 216, 403, 803, 865, 1296, 2039, 2928, 3102, 3126
FauNDI CATATG 2 cut(s) 2697, 3138
FbaI TGATCA 2 cut(s) 1243, 1855
FblI GTMKAC 4 cut(s) 1645, 2462, 2757, 2813
FriOI GRGCYC 3 cut(s) 484, 2047, 2236
GlaI GCGC 1 cut(s) 3125
GsaI CCCAGC 1 cut(s) 2084
GsuI CTGGAG 1 cut(s) 1520
HapII CCGG 6 cut(s) 268, 381, 1660, 2567, 2784, 2865
HgaI GACGC 1 cut(s) 190
HhaI GCGC 1 cut(s) 3126
Hin1II CATG 9 cut(s) 220, 407, 807, 869, 1300, 2043, 2932, 3106, 3130
Hin6I GCGC 1 cut(s) 3124
HinP1I GCGC 1 cut(s) 3124
HincII GTYRAC 3 cut(s) 2280, 2488, 2758
HindII GTYRAC 3 cut(s) 2280, 2488, 2758
HindIII AAGCTT 1 cut(s) 119
HpaI GTTAAC 2 cut(s) 2280, 2488
HpaII CCGG 6 cut(s) 268, 381, 1660, 2567, 2784, 2865
HphI GGTGA 4 cut(s) 755, 1061, 1352, 2156
Hpy99I CGWCG 1 cut(s) 134
HpyCH4III ACNGT 9 cut(s) 191, 727, 1448, 1522, 1693, 1724, 2002, 2267, 2983
HpyCH4IV ACGT 4 cut(s) 1144, 1776, 2493, 2810
HpySE526I ACGT 4 cut(s) 1144, 1776, 2493, 2810
Hsp92II CATG 9 cut(s) 220, 407, 807, 869, 1300, 2043, 2932, 3106, 3130
HspAI GCGC 1 cut(s) 3124
KflI GGGWCCC 2 cut(s) 1969, 2319
KpnI GGTACC 2 cut(s) 1902, 2504
KroI GCCGGC 1 cut(s) 267
KroNI GCCGGC 1 cut(s) 269
Ksp22I TGATCA 2 cut(s) 1243, 1855
KspAI GTTAAC 2 cut(s) 2280, 2488
LguI GCTCTTC 1 cut(s) 2919
LmnI GCTCC 8 cut(s) 1478, 1958, 2341, 2629, 2640, 2845, 3033, 3172
MabI ACCWGGT 1 cut(s) 1045
MaeII ACGT 4 cut(s) 1144, 1776, 2493, 2810
MaeIII GTNAC 8 cut(s) 146, 164, 394, 704, 982, 1195, 1259, 1634
MfeI CAATTG 3 cut(s) 615, 933, 2802
MflI RGATCY 4 cut(s) 256, 748, 1160, 3252
MhlI GDGCHC 7 cut(s) 484, 528, 1537, 2000, 2047, 2236, 2874
MmeI TCCRAC 4 cut(s) 342, 1303, 1430, 2286
MroNI GCCGGC 1 cut(s) 267
MroXI GAANNNNTTC 1 cut(s) 1401
MslI CAYNNNNRTG 5 cut(s) 1540, 1925, 1993, 2700, 3198
MspA1I CMGCKG 2 cut(s) 550, 2084
MspI CCGG 6 cut(s) 268, 381, 1660, 2567, 2784, 2865
MspR9I CCNGG 4 cut(s) 578, 1047, 1661, 2230
MunI CAATTG 3 cut(s) 615, 933, 2802
Mva1269I GAATGC 4 cut(s) 916, 1843, 2143, 2189
MvaI CCWGG 3 cut(s) 578, 1047, 2230
NaeI GCCGGC 1 cut(s) 269
NciI CCSGG 1 cut(s) 1661
NcoI CCATGG 3 cut(s) 865, 1296, 2928
NdeI CATATG 2 cut(s) 2697, 3138
NgoMIV GCCGGC 1 cut(s) 267
NlaIII CATG 9 cut(s) 220, 407, 807, 869, 1300, 2043, 2932, 3106, 3130
NmeAIII GCCGAG 3 cut(s) 379, 921, 1196
NmuCI GTSAC 4 cut(s) 146, 394, 704, 1259
NspI RCATGY 1 cut(s) 3106
OliI CACNNNNGTG 1 cut(s) 1993
PceI AGGCCT 2 cut(s) 345, 1343
PciSI GCTCTTC 1 cut(s) 2919
PctI GAATGC 4 cut(s) 916, 1843, 2143, 2189
PdiI GCCGGC 1 cut(s) 269
PdmI GAANNNNTTC 1 cut(s) 1401
PfoI TCCNGGA 1 cut(s) 1659
PpuMI RGGWCCY 4 cut(s) 430, 1969, 2077, 2319
PshAI GACNNNNGTC 1 cut(s) 711
PsiI TTATAA 2 cut(s) 1718, 3002
Psp5II RGGWCCY 4 cut(s) 430, 1969, 2077, 2319
Psp6I CCWGG 3 cut(s) 576, 1045, 2228
PspFI CCCAGC 1 cut(s) 2080
PspGI CCWGG 3 cut(s) 576, 1045, 2228
PspOMI GGGCCC 2 cut(s) 480, 2232
PspPPI RGGWCCY 4 cut(s) 430, 1969, 2077, 2319
PstI CTGCAG 2 cut(s) 1491, 1749
PstNI CAGNNNCTG 3 cut(s) 150, 1697, 1769
PsuI RGATCY 4 cut(s) 256, 748, 1160, 3252
PvuII CAGCTG 2 cut(s) 550, 2084
RsaI GTAC 8 cut(s) 284, 678, 1900, 2120, 2400, 2502, 2852, 2943
RsaNI GTAC 8 cut(s) 283, 677, 1899, 2119, 2399, 2501, 2851, 2942
RseI CAYNNNNRTG 5 cut(s) 1540, 1925, 1993, 2700, 3198
SalI GTCGAC 1 cut(s) 2756
SapI GCTCTTC 1 cut(s) 2919
ScrFI CCNGG 4 cut(s) 578, 1047, 1661, 2230
SduI GDGCHC 7 cut(s) 484, 528, 1537, 2000, 2047, 2236, 2874
SexAI ACCWGGT 1 cut(s) 1045
SfcI CTRYAG 3 cut(s) 582, 1487, 1745
SinI GGWCC 5 cut(s) 430, 713, 1969, 2077, 2319
SmiMI CAYNNNNRTG 5 cut(s) 1540, 1925, 1993, 2700, 3198
SmlI CTYRAG 3 cut(s) 824, 1232, 2069
SmoI CTYRAG 3 cut(s) 824, 1232, 2069
SseBI AGGCCT 2 cut(s) 345, 1343
SsiI CCGC 4 cut(s) 60, 209, 304, 3170
SspI AATATT 1 cut(s) 1735
StuI AGGCCT 2 cut(s) 345, 1343
StyD4I CCNGG 4 cut(s) 576, 1045, 1659, 2228
StyI CCWWGG 6 cut(s) 346, 865, 1002, 1296, 1903, 2928
TaaI ACNGT 9 cut(s) 191, 727, 1448, 1522, 1693, 1724, 2002, 2267, 2983
TaiI ACGT 4 cut(s) 1147, 1779, 2496, 2813
TaqI TCGA 7 cut(s) 533, 624, 810, 1386, 1419, 2577, 2757
TaqII GACCGA 1 cut(s) 890
TatI WGTACW 3 cut(s) 2118, 2398, 2850
TauI GCSGC 2 cut(s) 211, 307
TseFI GTSAC 4 cut(s) 146, 394, 704, 1259
Tsp45I GTSAC 4 cut(s) 146, 394, 704, 1259
TspDTI ATGAA 9 cut(s) 423, 915, 1174, 1994, 1998, 2147, 2624, 2669, 2767
TspGWI ACGGA 1 cut(s) 903
VneI GTGCAC 2 cut(s) 1533, 1996
VpaK11BI GGWCC 5 cut(s) 430, 713, 1969, 2077, 2319
XagI CCTNNNNNAGG 1 cut(s) 2192
XapI RAATTY 8 cut(s) 235, 620, 1013, 1464, 1558, 1801, 2507, 2774
XbaI TCTAGA 1 cut(s) 1825
XceI RCATGY 1 cut(s) 3106
XmaJI CCTAGG 1 cut(s) 1903
XmiI GTMKAC 4 cut(s) 1645, 2462, 2757, 2813
XmnI GAANNNNTTC 1 cut(s) 1401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.