Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0002217

Physical Location & Seq

Genomic Coordinates Forward (+)

12922340 .. 12957767

Gene Structure 35428 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00010290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3450 bp

ATGGTCAAATTAGGGTTAAAGTTTTGGGGGTGGGTTGCTGTAGCTTCGGCGCCGATCTTTGTCGGATTGGCGTTCCGAAGCTACTCCGGCGTCGGCGACGGTGCCGATTCGGTTGATTTTCCGGTTTTGTGTTTGGTTGTTTCGGTGATCGTCGGGTTTTGTGGCGTGTTCAGCAGCTTACTGCACTATATGCTTCCTAGAAAGAGACCTTGTGAAGGAGAAGAAGGAGACGGTGACATTAACAAGACCACTGGTACCAGCTCGGCGGCTTCTGCGTCTTTGATCAAGAAGCTTCGGATCGAATCCTACGTTGCCGAATCGGCCATCAACAACAGCAGTAACAACAGCAGCAGCAACGGTAGCGGGAGCGTGGTTGAGAACGACGTGCCGATCATGGCTTTGGGAGATTCCAACGCCGGAGATATCGATGAGGATCTGCACAGCCGGCAGCTTGCCGTCTATGGCCGGGAGACGATGAGGAGGCTCTTTGCATCCAATGTTCTGATCTCAGGGATACAGGGCCTCGGTGCCGAGATTGCCAAAAATCTCATTCTTGCTGGTGTGAAGTCTGTGACCTTGCATGATGAAGGGACTGTGGAGCTTTGGGATTTGTCCAGCAATTTTATTTTTACAGAGGATGATGTTGGTAAGAACAGGGCACTTGCTTCTGTTCAAAAGTTGCAGGAACTCAACAATGCTGTGATTGTTCATACCGTGACAACTCCATTGACTACGGAGCGGCTTTCTGATTTCCAGGCTGTTGTATTCACTGATATCAGTTATGAGAAAGCTATTGAATTCAATGATTATTGTCATAATCATCAGCCGCCAATCGCCTTCATAAGAACCGACGTTAGAGGCCTTTTTGGTTCTGTATTTTGTGACTTTGGACCTGAATTCACTGTTTTTGATGTTGATGGGGAGGAACCACACACGGGTATTATTGCATCAATCAGCAATGACAACCCTGCTCTTGTGTCATGTGTCGATGATGAAAGACTTGAATTTCAGGATGGGGATCTTGTTGTGTTCTCAGAAGTTCATGGAATGACAGAACTAAATGATGGAAAGCCAAGGAAGATAAAAAATGCTAGGGCTTACTCATTTACTCTTGAGGAGGATACCTCAGGTTTTGGTACCTATGTGAAAGGTGGCATTGTCACACAAGTGAAGCAGCCAAAAGTGTTGAACTTCAAGCCATTGGGGGAAGCACTCAAGAATCCCGGTGATTTTCTTCTTAGTGATTTCTCCAAGTTTGATCGCCCTCCTCTCTTACACTTGGCATTTCAAGCACTGGACAAATTTGTTTCTGAGTTGGGACGATTCCCTGTTGCTGGTTCAGAAGAGGATGCTCAGAAGCTTATATCTGTTGCTACTAACATTAATGATAAACTGGGAGATGGCAGGCTGGAAGATCTTAATCCAAAAATTTTGCGCCACTTCGCCTTTGGTGCAAAGGCGGTACTAAGTCCCATGGCTGCCATGTTTGGTGGTATCGTTGGTCAAGAGGTTGTAAAAGCTTGCTCTGGAAAGTTTCATCCACTCTTTCAGTTTTTCTACTTTGACTCGGTAGAATCACTTCCAACGGAGCCACTGGATTCCAGTGATTTGAAACCATTAAATAGCCGTTATGATGCACAGATTTCAGTTTTTGGGTCCAAGCTACAGAAGAAACTGGAGGATGCCAAAGTATTCATGGTTGGATCTGGTGCACTTGGATGTGAGTTGTTGAAAAATGTTGCCCTGATGGGAGTTTCATGTGGCAACCAAGGGAAGCTAACAATCACTGATGATGACGTAATTGAGAAGAGTAACCTCAGTAGGCAGTTCCTTTTCCGTGATTGGAACATTGGGCAGGCAAAGTCTACAGTTGCCGCTTCTGCTGCTGCATCAATAAATCCCCGTCTCAACATTGTAGCGTTGCAGAATAGAGTTGGTCCTGAAACTGAGAATGTGTTTGATGATACCTACTGGGAGAATTTAAGTGTTGTTATTAATGCACTAGATAATGTCAATGCCAGGCTCTATGTTGATCAGCGGTGCTTATATTTCCAGAAGGCACTTCTTGAGTCGGGTACTCTGGGTGCGAAATGCAATACTCAGATGGTTATTCCTCACCTAACAGAAAACTATGGTGCCTCTAGAGATCCTCCAGAGAAACAAGCACCCATGTGTACCGTCCACTCTTTTCCACACAACATTGACCACTGTTTGACATGGGCTCGATCTGAGTTTGAGGGTTTACTCGAGAAAACTCCAGCTGAAGTGAATGCATATTTATCCAAGCCAAGTGAATACACCGCTGCAATGAGTAATGCTGGGGATGCACAGGCCAGGGACACCTTGGAGCGTGTTCTCGAGTGCCTTTCCAGAGAAAGATGTGAAACATTCCAAGATTGTATTGCCTGGGCGCGCCTCAAGTTTGAAGATTATTTTTCCAACCGGGTGAAACAGCTGACATATACTTTCCCTGAAGATGCTGCAACTAGTACTGGGGCTCCATTCTGGTCTGCCCCCAAGCGATTCCCCCGTGCACTGCAGTTCTCAGCTAATGATCCCGGGCATCTCCATTTTGTTATGGCAGCATCCATACTCAGGGCAGAGACGTTTGGGATCCCAATCCCCGACTGGGTTAGGAACTCTAAGAAGTTGACTGAAGCTGTTGAGAAAGTGGAAGTCCCAGACTTTCAGCCCAAGAAAGATGCAAAAATAGTGACAGATGATAAGGCCACCAATTTAACTCCTCAATCTATAGATGATGCACAGGTTATTAATGAATTGATTATCAAGTTAGAGCATTGTCGAGAGAAACTCCCACCAGGATTCAGGATGAAACCGATTCAATTCGAGAAGGATGATGATGCCAATTACCATATGGATATGATAGCTGGGCTCGCCAATATGAGGGCTAGGAATTATAGCATTCCTGAGGTGGACAAGCTGAAAGCCAAGTTTATTGCCGGTAGGATTATCCCTGCAATTGCAACATCCACTGCAATGGCCACAGGACTTGTGTGCCTGGAACTTTACAAGGTTCTGGATGGCGGCCACAAACTTGAAGACTACAGAAATACATTTGCAAACCTAGCACTACCTTTGTTCTCCATGGCTGAGCCGGTACCTCCCAAGGTCATCAAGCACCAGGACATGAGCTGGACTGTGTGGGATAGGTGGATCCTGAGGGGCAATCCTACTCTGAGGGAACTTCTCCAGTGGCTTAAGGATAAGGGGCTGAATGCTTATAGTATCTCTTGTGGAAGCTCTCTGCTTTTTAACAGCATGTTTGCTCGACACAAAGACCGAATGGACAAGAAGGTGGTGGATCTGGCTAAGGACGTGGCGAAGGTGGAAATTCCTCCATATCGTTGCCATTTGGATGTCGTTGTTGCTTGCGAGGATGATGAGGACAATGACATTGACATCCCTCTGGTGTCAGTTTACTTCCGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030054 GO:0032446 GO:0033554 GO:0036211 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 ko05012 map04120 map05012

Pfam Domains

Protein Families

E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1149

Amino Acids

127.06

Weight (kDa)

5.27

Isoelectric Point (pI)

32.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	147 - 522	4e-27	ThiF family
E1_FCCH	PF16190	321 - 391	1.5e-28	Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB	PF16191	392 - 462	1.2e-25	Ubiquitin-activating enzyme E1 four-helix bundle
ThiF	PF00899	544 - 1036	1.1e-58	ThiF family
UBA_E1_SCCH	PF10585	731 - 984	2.2e-87	Ubiquitin-activating enzyme, SCCH domain
E1_UFD	PF09358	1055 - 1144	2.4e-23	Ubiquitin fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30110 AT5G06460
fragaria_vesca	FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_6g10930 FvH4_6g10930 FvH4_6g10930
malus_domestica	MD04G1163500.v1.1 MD11G1065700.v1.1 MD12G1177300.v1.1
prunus_persica	Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G282900_v2.0.a1
pyrus_communis	pycom04g14620 pycom11g05570 pycom12g16790
rosa_chinensis	RchiOBHm_Chr3g0462201 RchiOBHm_Chr5g0070911
rosa_laevigata	RLG00000024878 RLG00000036170
rosa_multiflora	Rmu_sc0002944.1_g000004 Rmu_sc0005970.1_g000009 Rmu_sc0006715.1_g000003
rosa_roxburghii	Rroxscaffold_1G00010290 Rroxscaffold_6G00417690
rosa_rugosa	Rorug03G0054900 Rorug05G0408500 Rorug05G0408500 Rorug05G0408500
rosa_samantha	Rh3CG119500 Rh5AG464600 Rh5DG494900
rosa_wichuraiana	Rw3G009590 Rw5G043220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	3 cut(s)	254, 1136, 3118
AccB1I	GGYRCC	7 cut(s)	49, 101, 254, 527, 1136, 2135, 3118
AccBSI	CCGCTC	1 cut(s)	739
AccI	GTMKAC	1 cut(s)	1865
AccII	CGCG	1 cut(s)	2413
AciI	CCGC	9 cut(s)	266, 363, 739, 827, 1460, 1875, 2038, 2301, 3045
AcoI	YGGCCR	4 cut(s)	321, 463, 3000, 3046
AcsI	RAATTY	7 cut(s)	797, 896, 1004, 1301, 1428, 1978, 3351
AcuI	CTGAAG	3 cut(s)	2283, 2493, 2676
AcyI	GRCGYC	2 cut(s)	50, 90
AfaI	GTAC	7 cut(s)	256, 1138, 1464, 2077, 2176, 2491, 3120
AfiI	CCNNNNNNNGG	6 cut(s)	215, 935, 1334, 2595, 2629, 2904
AflII	CTTAAG	1 cut(s)	3218
AhlI	ACTAGT	1 cut(s)	2486
AjiI	CACGTC	2 cut(s)	385, 3337
AjnI	CCWGG	7 cut(s)	753, 2018, 2333, 2405, 2818, 3018, 3141
AleI	CACNNNNGTG	2 cut(s)	1166, 2170
Alw21I	GWGCWC	2 cut(s)	1714, 2536
Alw26I	GTCTC	5 cut(s)	199, 222, 464, 1910, 2597
Alw44I	GTGCAC	2 cut(s)	1710, 2532
Ama87I	CYCGRG	3 cut(s)	2246, 2357, 2558
AoxI	GGCC	8 cut(s)	321, 463, 520, 859, 2331, 2727, 3000, 3046
ApaLI	GTGCAC	2 cut(s)	1710, 2532
ApoI	RAATTY	7 cut(s)	797, 896, 1004, 1301, 1428, 1978, 3351
AscI	GGCGCGCC	1 cut(s)	2411
AseI	ATTAAT	3 cut(s)	1383, 1995, 2772
Asp700I	GAANNNNTTC	1 cut(s)	1578
Asp718I	GGTACC	3 cut(s)	254, 1136, 3118
AspLEI	GCGC	4 cut(s)	52, 1437, 2413, 2415
AspS9I	GGNCC	4 cut(s)	520, 890, 1656, 1937
AsuC2I	CCSGG	5 cut(s)	467, 1224, 2444, 2559, 2560
AsuHPI	GGTGA	5 cut(s)	157, 245, 1238, 2108, 2458
AvaI	CYCGRG	3 cut(s)	2246, 2357, 2558
AvaII	GGWCC	3 cut(s)	890, 1656, 1937
AxyI	CCTNAGG	3 cut(s)	1126, 2928, 3179
BaeGI	GKGCMC	3 cut(s)	661, 1714, 2536
BaeI	ACNNNNGTAYC	2 cut(s)	1128, 1161
BalI	TGGCCA	1 cut(s)	3002
BamHI	GGATCC	2 cut(s)	2613, 3174
BanI	GGYRCC	7 cut(s)	49, 101, 254, 527, 1136, 2135, 3118
BanII	GRGCYC	3 cut(s)	2224, 2500, 2895
BarI	GAAGNNNNNNTAC	2 cut(s)	1563, 1595
BbsI	GAAGAC	1 cut(s)	3066
Bbv12I	GWGCWC	2 cut(s)	1714, 2536
BccI	CCATC	8 cut(s)	332, 911, 1007, 1058, 1394, 1741, 2098, 3035
BceAI	ACGGC	2 cut(s)	440, 1611
BciT130I	CCWGG	7 cut(s)	755, 2020, 2335, 2407, 2820, 3020, 3143
BciVI	GTATCC	2 cut(s)	507, 1114
BclI	TGATCA	2 cut(s)	282, 2032
BcnI	CCSGG	5 cut(s)	467, 1224, 2444, 2559, 2560
BcoDI	GTCTC	5 cut(s)	199, 222, 464, 1910, 2597
BcuI	ACTAGT	1 cut(s)	2486
BfaI	CTAG	7 cut(s)	198, 1092, 2003, 2142, 2487, 2910, 3086
BfmI	CTRYAG	6 cut(s)	39, 1664, 1866, 2537, 2751, 3064
BfoI	RGCGCY	1 cut(s)	53
BfrI	CTTAAG	1 cut(s)	3218
BfuI	GTATCC	2 cut(s)	507, 1114
BglI	GCCNNNNNGGC	1 cut(s)	320
BglII	AGATCT	1 cut(s)	1414
BlpI	GCTNAGC	1 cut(s)	3111
BmcAI	AGTACT	1 cut(s)	2491
Bme18I	GGWCC	3 cut(s)	890, 1656, 1937
BmeT110I	CYCGRG	3 cut(s)	2246, 2357, 2558
BmgBI	CACGTC	2 cut(s)	385, 3337
BmgT120I	GGNCC	4 cut(s)	520, 890, 1656, 1937
BmrI	ACTGGG	4 cut(s)	1403, 1981, 2502, 2638
BmuI	ACTGGG	4 cut(s)	1403, 1981, 2502, 2638
BpiI	GAAGAC	1 cut(s)	3066
BplI	GAGNNNNNCTC	4 cut(s)	1109, 1141, 2796, 2828
BpmI	CTGGAG	4 cut(s)	1697, 2136, 2241, 3194
Bpu10I	CCTNAGC	1 cut(s)	3330
Bpu1102I	GCTNAGC	1 cut(s)	3111
BpuEI	CTTGAG	4 cut(s)	1133, 1199, 2087, 2402
BpuMI	CCSGG	5 cut(s)	467, 1224, 2444, 2559, 2560
Bsa29I	ATCGAT	1 cut(s)	426
BsaBI	GATNNNNATC	4 cut(s)	432, 1017, 1419, 2618
BsaHI	GRCGYC	2 cut(s)	50, 90
BsaI	GGTCTC	1 cut(s)	199
BsaWI	WCCGGW	1 cut(s)	121
BsaXI	ACNNNNNCTCC	2 cut(s)	2482, 2512
Bsc4I	CCNNNNNNNGG	6 cut(s)	215, 935, 1334, 2595, 2629, 2904
Bse118I	RCCGGY	3 cut(s)	444, 2960, 3115
Bse21I	CCTNAGG	3 cut(s)	1126, 2928, 3179
Bse3DI	GCAATG	3 cut(s)	964, 2313, 3003
Bse8I	GATNNNNATC	4 cut(s)	432, 1017, 1419, 2618
BseBI	CCWGG	7 cut(s)	755, 2020, 2335, 2407, 2820, 3020, 3143
BseCI	ATCGAT	1 cut(s)	426
BseJI	GATNNNNATC	4 cut(s)	432, 1017, 1419, 2618
BseLI	CCNNNNNNNGG	6 cut(s)	215, 935, 1334, 2595, 2629, 2904
BseMI	GCAATG	3 cut(s)	964, 2313, 3003
BsePI	GCGCGC	1 cut(s)	2411
BseRI	GAGGAG	4 cut(s)	493, 1130, 1257, 2733
BseSI	GKGCMC	3 cut(s)	661, 1714, 2536
BseYI	CCCAGC	2 cut(s)	2318, 2888
BsgI	GTGCAG	2 cut(s)	167, 422
Bsh1236I	CGCG	1 cut(s)	2413
BshFI	GGCC	8 cut(s)	323, 465, 522, 861, 2333, 2729, 3002, 3048
BshNI	GGYRCC	7 cut(s)	49, 101, 254, 527, 1136, 2135, 3118
BshVI	ATCGAT	1 cut(s)	426
BsiHKAI	GWGCWC	2 cut(s)	1714, 2536
BsiHKCI	CYCGRG	3 cut(s)	2246, 2357, 2558
BslFI	GGGAC	5 cut(s)	604, 1332, 1455, 2351, 2663
BslI	CCNNNNNNNGG	6 cut(s)	215, 935, 1334, 2595, 2629, 2904
BsmAI	GTCTC	5 cut(s)	199, 222, 464, 1910, 2597
BsmBI	CGTCTC	4 cut(s)	222, 464, 1910, 2597
BsmFI	GGGAC	5 cut(s)	604, 1332, 1455, 2351, 2663
BsmI	GAATGC	3 cut(s)	2275, 2922, 3241
BsnI	GGCC	8 cut(s)	323, 465, 522, 861, 2333, 2729, 3002, 3048
Bso31I	GGTCTC	1 cut(s)	199
BsoBI	CYCGRG	3 cut(s)	2246, 2357, 2558
Bsp1286I	GDGCHC	6 cut(s)	661, 1714, 2224, 2500, 2536, 2895
Bsp1720I	GCTNAGC	1 cut(s)	3111
Bsp19I	CCATGG	2 cut(s)	1473, 3105
BspACI	CCGC	9 cut(s)	266, 363, 739, 827, 1460, 1875, 2038, 2301, 3045
BspANI	GGCC	8 cut(s)	323, 465, 522, 861, 2333, 2729, 3002, 3048
BspDI	ATCGAT	1 cut(s)	426
BspFNI	CGCG	1 cut(s)	2413
BspMAI	CTGCAG	1 cut(s)	2541
BspT107I	GGYRCC	7 cut(s)	49, 101, 254, 527, 1136, 2135, 3118
BspTI	CTTAAG	1 cut(s)	3218
BspTNI	GGTCTC	1 cut(s)	199
BsrBI	CCGCTC	1 cut(s)	739
BsrDI	GCAATG	3 cut(s)	964, 2313, 3003
BsrFI	RCCGGY	3 cut(s)	444, 2960, 3115
BssAI	RCCGGY	3 cut(s)	444, 2960, 3115
BssHII	GCGCGC	1 cut(s)	2411
BssNI	GRCGYC	2 cut(s)	50, 90
BssT1I	CCWWGG	6 cut(s)	1073, 1473, 1768, 2343, 3105, 3126
Bst2UI	CCWGG	7 cut(s)	755, 2020, 2335, 2407, 2820, 3020, 3143
Bst6I	CTCTTC	2 cut(s)	1338, 1802
BstACI	GRCGYC	2 cut(s)	50, 90
BstAFI	CTTAAG	1 cut(s)	3218
BstAPI	GCANNNNNTGC	1 cut(s)	190
BstC8I	GCNNGC	8 cut(s)	446, 453, 1406, 1522, 1857, 2413, 2895, 3391
BstDSI	CCRYGG	2 cut(s)	1473, 3105
BstENI	CCTNNNNNAGG	1 cut(s)	213
BstFNI	CGCG	1 cut(s)	2413
BstH2I	RGCGCY	1 cut(s)	53
BstHHI	GCGC	4 cut(s)	52, 1437, 2413, 2415
BstMAI	GTCTC	5 cut(s)	199, 222, 464, 1910, 2597
BstNI	CCWGG	7 cut(s)	755, 2020, 2335, 2407, 2820, 3020, 3143
BstNSI	RCATGY	1 cut(s)	3283
BstSFI	CTRYAG	6 cut(s)	39, 1664, 1866, 2537, 2751, 3064
BstSLI	GKGCMC	3 cut(s)	661, 1714, 2536
BstUI	CGCG	1 cut(s)	2413
BstV2I	GAAGAC	1 cut(s)	3066
BstX2I	RGATCY	8 cut(s)	433, 1018, 1414, 1703, 2146, 2613, 3174, 3322
BstXI	CCANNNNNNTGG	1 cut(s)	2998
BstYI	RGATCY	8 cut(s)	433, 1018, 1414, 1703, 2146, 2613, 3174, 3322
Bsu15I	ATCGAT	1 cut(s)	426
Bsu36I	CCTNAGG	3 cut(s)	1126, 2928, 3179
BsuI	GTATCC	2 cut(s)	507, 1114
BsuRI	GGCC	8 cut(s)	323, 465, 522, 861, 2333, 2729, 3002, 3048
BsuTUI	ATCGAT	1 cut(s)	426
BtgI	CCRYGG	2 cut(s)	1473, 3105
BtrI	CACGTC	2 cut(s)	385, 3337
BtsI	GCAGTG	2 cut(s)	2534, 2991
Cac8I	GCNNGC	8 cut(s)	446, 453, 1406, 1522, 1857, 2413, 2895, 3391
CfoI	GCGC	4 cut(s)	52, 1437, 2413, 2415
Cfr10I	RCCGGY	3 cut(s)	444, 2960, 3115
Cfr13I	GGNCC	4 cut(s)	520, 890, 1656, 1937
Cfr9I	CCCGGG	1 cut(s)	2558
ClaI	ATCGAT	1 cut(s)	426
CseI	GACGC	2 cut(s)	79, 264
Csp6I	GTAC	7 cut(s)	255, 1137, 1463, 2076, 2175, 2490, 3119
CspCI	CAANNNNNGTGG	2 cut(s)	2992, 3027
CviQI	GTAC	7 cut(s)	255, 1137, 1463, 2076, 2175, 2490, 3119
DinI	GGCGCC	1 cut(s)	51
EaeI	YGGCCR	4 cut(s)	321, 463, 3000, 3046
Eam1104I	CTCTTC	2 cut(s)	1338, 1802
EarI	CTCTTC	2 cut(s)	1338, 1802
Eco130I	CCWWGG	6 cut(s)	1073, 1473, 1768, 2343, 3105, 3126
Eco147I	AGGCCT	1 cut(s)	861
Eco24I	GRGCYC	3 cut(s)	2224, 2500, 2895
Eco31I	GGTCTC	1 cut(s)	199
Eco32I	GATATC	2 cut(s)	424, 775
Eco47I	GGWCC	3 cut(s)	890, 1656, 1937
Eco57I	CTGAAG	3 cut(s)	2283, 2493, 2676
Eco81I	CCTNAGG	3 cut(s)	1126, 2928, 3179
Eco88I	CYCGRG	3 cut(s)	2246, 2357, 2558
EcoNI	CCTNNNNNAGG	1 cut(s)	213
EcoO109I	RGGNCCY	1 cut(s)	520
EcoRI	GAATTC	2 cut(s)	797, 896
EcoRII	CCWGG	7 cut(s)	753, 2018, 2333, 2405, 2818, 3018, 3141
EcoRV	GATATC	2 cut(s)	424, 775
EcoT14I	CCWWGG	6 cut(s)	1073, 1473, 1768, 2343, 3105, 3126
EcoT22I	ATGCAT	1 cut(s)	2275
EcoT38I	GRGCYC	3 cut(s)	2224, 2500, 2895
EgeI	GGCGCC	1 cut(s)	51
EheI	GGCGCC	1 cut(s)	51
ErhI	CCWWGG	6 cut(s)	1073, 1473, 1768, 2343, 3105, 3126
Esp3I	CGTCTC	4 cut(s)	222, 464, 1910, 2597
FaqI	GGGAC	5 cut(s)	604, 1332, 1455, 2351, 2663
FauI	CCCGC	1 cut(s)	356
FauNDI	CATATG	1 cut(s)	2874
FbaI	TGATCA	2 cut(s)	282, 2032
FblI	GTMKAC	1 cut(s)	1865
FriOI	GRGCYC	3 cut(s)	2224, 2500, 2895
FspBI	CTAG	7 cut(s)	198, 1092, 2003, 2142, 2487, 2910, 3086
GlaI	GCGC	4 cut(s)	51, 1436, 2412, 2414
GsaI	CCCAGC	2 cut(s)	2322, 2892
GsuI	CTGGAG	4 cut(s)	1697, 2136, 2241, 3194
HaeII	RGCGCY	1 cut(s)	53
HaeIII	GGCC	8 cut(s)	323, 465, 522, 861, 2333, 2729, 3002, 3048
HgaI	GACGC	2 cut(s)	79, 264
HhaI	GCGC	4 cut(s)	52, 1437, 2413, 2415
Hin1I	GRCGYC	2 cut(s)	50, 90
Hin6I	GCGC	4 cut(s)	50, 1435, 2411, 2413
HinP1I	GCGC	4 cut(s)	50, 1435, 2411, 2413
HincII	GTYRAC	1 cut(s)	2652
HindII	GTYRAC	1 cut(s)	2652
HindIII	AAGCTT	3 cut(s)	290, 1358, 1518
HphI	GGTGA	5 cut(s)	157, 245, 1238, 2108, 2458
Hpy166II	GTNNAC	9 cut(s)	1712, 1866, 2175, 2182, 2243, 2534, 2652, 2935, 3439
Hpy8I	GTNNAC	9 cut(s)	1712, 1866, 2175, 2182, 2243, 2534, 2652, 2935, 3439
Hpy99I	CGWCG	5 cut(s)	95, 101, 155, 386, 854
HpyAV	CCTTC	8 cut(s)	209, 218, 581, 847, 2050, 2845, 3307, 3337
HpyCH4IV	ACGT	6 cut(s)	309, 384, 852, 1797, 2606, 3336
HpySE526I	ACGT	6 cut(s)	309, 384, 852, 1797, 2606, 3336
Hsp92I	GRCGYC	2 cut(s)	50, 90
HspAI	GCGC	4 cut(s)	50, 1435, 2411, 2413
KasI	GGCGCC	1 cut(s)	49
KpnI	GGTACC	3 cut(s)	258, 1140, 3122
KroI	GCCGGC	1 cut(s)	444
KroNI	GCCGGC	1 cut(s)	446
Ksp22I	TGATCA	2 cut(s)	282, 2032
LmnI	GCTCC	6 cut(s)	366, 598, 736, 1588, 2347, 2503
MaeI	CTAG	7 cut(s)	198, 1092, 2003, 2142, 2487, 2910, 3086
MaeII	ACGT	6 cut(s)	309, 384, 852, 1797, 2606, 3336
MaeIII	GTNAC	8 cut(s)	233, 338, 571, 715, 881, 1159, 1811, 2713
MbiI	CCGCTC	1 cut(s)	739
MfeI	CAATTG	1 cut(s)	2979
MflI	RGATCY	8 cut(s)	433, 1018, 1414, 1703, 2146, 2613, 3174, 3322
MhlI	GDGCHC	6 cut(s)	661, 1714, 2224, 2500, 2536, 2895
MlsI	TGGCCA	1 cut(s)	3002
MluNI	TGGCCA	1 cut(s)	3002
Mly113I	GGCGCC	1 cut(s)	50
MlyI	GAGTC	2 cut(s)	1559, 2078
MmeI	TCCRAC	5 cut(s)	43, 435, 1607, 1681, 2463
Mox20I	TGGCCA	1 cut(s)	3002
Mph1103I	ATGCAT	1 cut(s)	2275
MroNI	GCCGGC	1 cut(s)	444
MroXI	GAANNNNTTC	1 cut(s)	1578
MscI	TGGCCA	1 cut(s)	3002
MslI	CAYNNNNRTG	5 cut(s)	1166, 1717, 2170, 2996, 3375
Msp20I	TGGCCA	1 cut(s)	3002
MspA1I	CMGCKG	4 cut(s)	2038, 2261, 2303, 2455
MspCI	CTTAAG	1 cut(s)	3218
MunI	CAATTG	1 cut(s)	2979
Mva1269I	GAATGC	3 cut(s)	2275, 2922, 3241
MvaI	CCWGG	7 cut(s)	755, 2020, 2335, 2407, 2820, 3020, 3143
MvnI	CGCG	1 cut(s)	2413
NaeI	GCCGGC	1 cut(s)	446
NarI	GGCGCC	1 cut(s)	50
NciI	CCSGG	5 cut(s)	467, 1224, 2444, 2559, 2560
NcoI	CCATGG	2 cut(s)	1473, 3105
NdeI	CATATG	1 cut(s)	2874
NgoMIV	GCCGGC	1 cut(s)	444
NmeAIII	GCCGAG	2 cut(s)	242, 556
NmuCI	GTSAC	6 cut(s)	233, 571, 715, 881, 1159, 2713
NsiI	ATGCAT	1 cut(s)	2275
NspI	RCATGY	1 cut(s)	3283
OliI	CACNNNNGTG	2 cut(s)	1166, 2170
PaeR7I	CTCGAG	2 cut(s)	2246, 2357
PalAI	GGCGCGCC	1 cut(s)	2411
PauI	GCGCGC	1 cut(s)	2411
PceI	AGGCCT	1 cut(s)	861
PcsI	WCGNNNNNNNCGW	1 cut(s)	306
PctI	GAATGC	3 cut(s)	2275, 2922, 3241
PdiI	GCCGGC	1 cut(s)	446
PdmI	GAANNNNTTC	1 cut(s)	1578
PleI	GAGTC	2 cut(s)	1559, 2077
PluTI	GGCGCC	1 cut(s)	53
PpsI	GAGTC	2 cut(s)	1559, 2077
PshBI	ATTAAT	3 cut(s)	1383, 1995, 2772
Psp6I	CCWGG	7 cut(s)	753, 2018, 2333, 2405, 2818, 3018, 3141
PspFI	CCCAGC	2 cut(s)	2318, 2888
PspGI	CCWGG	7 cut(s)	753, 2018, 2333, 2405, 2818, 3018, 3141
PspPI	GGNCC	4 cut(s)	520, 890, 1656, 1937
PstI	CTGCAG	1 cut(s)	2541
PsuI	RGATCY	8 cut(s)	433, 1018, 1414, 1703, 2146, 2613, 3174, 3322
PteI	GCGCGC	1 cut(s)	2411
PvuII	CAGCTG	2 cut(s)	2261, 2455
RsaI	GTAC	7 cut(s)	256, 1138, 1464, 2077, 2176, 2491, 3120
RsaNI	GTAC	7 cut(s)	255, 1137, 1463, 2076, 2175, 2490, 3119
RseI	CAYNNNNRTG	5 cut(s)	1166, 1717, 2170, 2996, 3375
Sau96I	GGNCC	4 cut(s)	520, 890, 1656, 1937
ScaI	AGTACT	1 cut(s)	2491
SchI	GAGTC	2 cut(s)	1559, 2078
SduI	GDGCHC	6 cut(s)	661, 1714, 2224, 2500, 2536, 2895
SfcI	CTRYAG	6 cut(s)	39, 1664, 1866, 2537, 2751, 3064
SfoI	GGCGCC	1 cut(s)	51
Sfr274I	CTCGAG	2 cut(s)	2246, 2357
SgsI	GGCGCGCC	1 cut(s)	2411
SinI	GGWCC	3 cut(s)	890, 1656, 1937
SlaI	CTCGAG	2 cut(s)	2246, 2357
SmaI	CCCGGG	1 cut(s)	2560
SmiMI	CAYNNNNRTG	5 cut(s)	1166, 1717, 2170, 2996, 3375
SmlI	CTYRAG	7 cut(s)	1112, 1214, 2066, 2246, 2357, 2417, 3218
SmoI	CTYRAG	7 cut(s)	1112, 1214, 2066, 2246, 2357, 2417, 3218
SpeI	ACTAGT	1 cut(s)	2486
SseBI	AGGCCT	1 cut(s)	861
SsiI	CCGC	9 cut(s)	266, 363, 739, 827, 1460, 1875, 2038, 2301, 3045
SspDI	GGCGCC	1 cut(s)	49
SspMI	CTAG	7 cut(s)	198, 1092, 2003, 2142, 2487, 2910, 3086
StuI	AGGCCT	1 cut(s)	861
StyI	CCWWGG	6 cut(s)	1073, 1473, 1768, 2343, 3105, 3126
TaiI	ACGT	6 cut(s)	312, 387, 855, 1800, 2609, 3339
TaqI	TCGA	9 cut(s)	300, 426, 987, 2224, 2247, 2358, 2803, 2847, 3289
TaqII	GACCGA	1 cut(s)	3315
TatI	WGTACW	1 cut(s)	2489
TauI	GCSGC	5 cut(s)	269, 742, 829, 1877, 3048
TseFI	GTSAC	6 cut(s)	233, 571, 715, 881, 1159, 2713
Tsp45I	GTSAC	6 cut(s)	233, 571, 715, 881, 1159, 2713
TspGWI	ACGGA	4 cut(s)	749, 1601, 1826, 3434
TspMI	CCCGGG	1 cut(s)	2558
Vha464I	CTTAAG	1 cut(s)	3218
VneI	GTGCAC	2 cut(s)	1710, 2532
VpaK11BI	GGWCC	3 cut(s)	890, 1656, 1937
VspI	ATTAAT	3 cut(s)	1383, 1995, 2772
XagI	CCTNNNNNAGG	1 cut(s)	213
XapI	RAATTY	7 cut(s)	797, 896, 1004, 1301, 1428, 1978, 3351
XbaI	TCTAGA	1 cut(s)	2141
XceI	RCATGY	1 cut(s)	3283
XcmI	CCANNNNNNNNNTGG	5 cut(s)	1445, 1693, 2341, 2625, 2872
XhoI	CTCGAG	2 cut(s)	2246, 2357
XmaI	CCCGGG	1 cut(s)	2558
XmiI	GTMKAC	1 cut(s)	1865
XmnI	GAANNNNTTC	1 cut(s)	1578
XspI	CTAG	7 cut(s)	198, 1092, 2003, 2142, 2487, 2910, 3086
ZrmI	AGTACT	1 cut(s)	2491
Zsp2I	ATGCAT	1 cut(s)	2275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.