Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0002217

Physical Location & Seq

Genomic Coordinates Forward (+)

5663234 .. 5668393

Gene Structure 5160 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1065700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3447 bp

ATGCCCAAATTACGCTTTCTCATAGTGGCTTCGGCGGCCCCAATCCTTCTTGGTCTGGCGCTCCGAAAATACTCCGGCGCCGACGACGACGCCGCCAATTCAATTTTCACCACTCGTTTTCTCGTTTGGAGCTTGCTGGTTTCGGTGATCGTGGCGTTTTCTGGCGTTTTCAGCAGATTGCTGCACTGTATGCTTCCTAAAAAGAGACCTGGGGATGGAGTCGTTGTAGAAGTAGAAGTAGAAGTAGGAGGAGACGCCGGCAGCTCCGCCCCGTCATCGTCGATCAATAAGCATCGGATCGGCCACTTCGTTGCCGAATCTACTGTCAGCAACAACAGCAGCCTCGCAAGCAGCAACAACGGTAGCGTGGTTGAGAAAGACGTGCCGATCATGGCAGTGGGCGATTCGAACCAGTCCGATATCGACGAGGACCTTCACAGCCGGCAGCTCGCGGTCTATGGCCGCGAGACGATGCGGCGGCTCTTCGCGTCGAATGTTCTCATCTCAGGGATTCAAGGTCTAGGTGCTGAAATTGCAAAGAACCTCATTCTGGCTGGGGTCAAGTCTGTGACTTTGCATGATGAAGGGAATGTGGAGCTATGGGATTTGTCTAGTAACTTTGTTTTTTCAGAGGACGATGTGGGTAAGAACAGAGCACTTGCTTCGGTTCAAAAGTTGCAGGAACTCAACAATGCTGTGGTTGTTAATACCTTGACAACTGAATTGACTAAAGAACAGCTTTCTAATTTCCAGGTTGTTGTATTCACTGACATCAATCTCGAAAAAGCCATTGAATTCGATGATTATACGCATAATCATCAGCCCCCAATCGCCTTCATTAAAACTGAAGCTAGAGGCCTTTTTGGTTCTGTCTTTTGTGACTTTGGACCTGAGTTTACTGTTTTTGATGTTGATGGGGAGGAACCACACACGGGTATAATTGCATCCATCAGCAATGACAACCCTGCTATAGTGTCATGTGTTGACGACGAGAGACTTGAATTTCAGGATGGGGATCTTGTTGTGTTCTCAGAGGTTCATGGAATGACAGAACTAAATGATGGAAAACCAAGAAGGATAAAAAAGGCTAGGGCTTACTCATTCACTCTTGAGGAGGATACCACGAGGTTTGGTACCTATGAGAAAGGTGGTATTGTCACACAGGTGAAGCAGCCAAAAGTCTTGAAATTTAAGCCATTGAGAGAAGCACTCAATGATCCTGGTGAATTTCTTCCCAGTGATTTCTCCAAGTTTGATCGGCCACCACTCCTACACTTGACATTTCAAGCACTGGACAAAGTTGTGTCTGAGCTTGGGCGATTCCCTGTTGCTGGTTCAGAAGAGGATGCGCAGAAGCTTATATCTATTGCTAGTAACATTAATGAAAAATTGGGAGATGGTAAGATGGAAGATATTAATCCAAAACTTTTGCGGCACTTTGCCTTTGGTGCAAAGGCGGTACTGAATCCCATGGCTGCCATGTTTGGTGGTATTGTGGGTCAAGAGGTTGTAAAAGCTTGCTCTGGAAAGTTCCATCCACTCTTTCAGTTCTTCTACTTCGACTCAGTAGAATCACTTCCTACAGAGCCACTGGAATCCAGTGATTTGAAACCATTAAATAGCCGTTATGATGCACAAATTTCAGTTTTTGGGTCCAAGCTACAGAAGAAATTGGAAGATGCCAAAATATTTCTAGTTGGATCTGGGGCACTAGGATGTGAGTTCTTAAAAAATTTGGCCCTGATGGGAGTTTCATGTGGCAAGCAAGGGAAGCTAACAGTCACTGATGATGATGTAATTGAGAAGAGTAACCTTAGTAGGCAGTTCCTCTTCCGTGATTGGAACATTGGGCAGCCCAAATCCACGGCTGCTGCCTCCGCTGCTGCATCTATAAATCCTTATCTCAATGTCGAAGCCTTGCAGAATAGAGTTAGCTCTGAAACTGAGGATGTGTTTGATGATGCCTTCTGGGAGAATTTAAGTGTTGTTATCAATGCACTCGATAATGTCAATGCAAGGCTCTATGTTGATCAGAGGTGCTTATATTTCCAGAAGCCACTTCTCGAGTCAGGTACACTGGGCACAAAATGCAACACTCAGATGGTTATTCCTCCCCTAACAGAAAACTATGGTGCCTCAAGAGACCCACCGGAGAAGCAAGCACCCATGTGCACTTTGCACTCATTTCCACACAACATTGATCACTGCTTGACATGGGCTCGATCTGAGTTTGAGGGCTTGCTTGGCAAAACTCCAGCTGAAGTGAATGCGTATTTAGCCAAGCCAAGTGAGTATGCTGCTTCGAAGAGGAATGCTGGTGACGCACAGGCCAGGGATACGTTGGAGCGCATTCTTGAGTGCCTTGACAGAGAAAGATGCGAAACATTTCAAGATTGCATTGCATGGGCACGCCTGAAGTTTGAAGATTATTTTTCCAACCGCATCAAACAGCTGATCTATACTTTTCCTGAAGATGCTACAACCAGTAGTGGGACTCCATTCTGGTCTGCCCCTAAGCGGTTCCCCCATCCACTGCAGTTCTCAACTGCTGATCCCGGTCACCTTAATTTTGTTATGGCAGCATCCATACTAAAGGCAGAAACATTTGGGATCCCAATCCCCGACTGGGTTAGAAACTCTAAGAAGTTTGCTGAAGCTGTTGAGAAAGTGATGGTCCCAGAATTTCAGCCAAGGAGAGATGCAAACATAGTGACCGATGATAATGCTACCAATTTAACTTCTCAATCTACAGATGATGCACAGGTCATTGATGACTTGATCATCAAGTTAGAGCATTGTCGAGAGAAACTCCCACCAGGGTTCAGGATGAAACCAATTCAGTTCGAGAAGGACGATGATACAAATTACCATATGGATCTGATAGCTGGGCTTGCCAATATGAGGGCCAGAAATTACGGTATTCCTGAGGTTGACAAGCTGAAAGCCAAGTTTATTGCCGGCAAGATTATCCCCGCGATTGCTACCACCACCGCAATGGCCACAGGACTGGTGTGCCTGGAGCTTTACAAAGTTTTGGACGGAGGCCACAAACTAGAAGACTATAGAAACACGTTTGCAAATCTAGCTCTACCTTTGTTCGCCATGGCTGAGCCAGTTCCAGCGAAGGTCATCAAGCACCGTGACATGAGCTGGACCATTTGGGATAGGTGGATCGTGAGGGGCAATCCCACTCTGAGGGAATTTATTCAGTGGCTCAAGGACAGAGGACTGGATGCTTATAGCATCTCATATGCCAGTTCCCTGCTTTATAATACCATGTTTGCACGACACCAAGACCGAATGGACAAGAAGATGGTGGATCTGGCTAGGGAAGTGGGGGGTCTGGAACTGTCTCCAAACCGCTCCCACTTTGATGTTGTTGTGGCTTGTGAGGACGATGAAGGGGACGAAGTTGACATCCCTCTGGTATCCATCTACTTCCGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030054 GO:0032446 GO:0033554 GO:0036211 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 ko05012 map04120 map05012

Pfam Domains

Protein Families

E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1149

Amino Acids

127.53

Weight (kDa)

5.24

Isoelectric Point (pI)

34.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	146 - 522	1.6e-25	ThiF family
E1_FCCH	PF16190	320 - 390	8.4e-28	Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB	PF16191	391 - 461	1.6e-23	Ubiquitin-activating enzyme E1 four-helix bundle
ThiF	PF00899	543 - 1034	1e-61	ThiF family
UBA_E1_SCCH	PF10585	730 - 983	1.1e-87	Ubiquitin-activating enzyme, SCCH domain
E1_UFD	PF09358	1054 - 1143	9e-22	Ubiquitin fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30110 AT5G06460
fragaria_vesca	FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_6g10930 FvH4_6g10930 FvH4_6g10930
malus_domestica	MD04G1163500.v1.1 MD11G1065700.v1.1 MD12G1177300.v1.1
prunus_persica	Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G282900_v2.0.a1
pyrus_communis	pycom04g14620 pycom11g05570 pycom12g16790
rosa_chinensis	RchiOBHm_Chr3g0462201 RchiOBHm_Chr5g0070911
rosa_laevigata	RLG00000024878 RLG00000036170
rosa_multiflora	Rmu_sc0002944.1_g000004 Rmu_sc0005970.1_g000009 Rmu_sc0006715.1_g000003
rosa_roxburghii	Rroxscaffold_1G00010290 Rroxscaffold_6G00417690
rosa_rugosa	Rorug03G0054900 Rorug05G0408500 Rorug05G0408500 Rorug05G0408500
rosa_samantha	Rh3CG119500 Rh5AG464600 Rh5DG494900
rosa_wichuraiana	Rw3G009590 Rw5G043220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3270
Acc16I	TGCGCA	1 cut(s)	1350
Acc65I	GGTACC	1 cut(s)	1133
AccB1I	GGYRCC	3 cut(s)	77, 1133, 2132
AccBSI	CCGCTC	1 cut(s)	3363
AccII	CGCG	4 cut(s)	452, 465, 488, 2975
AcoI	YGGCCR	4 cut(s)	301, 460, 1259, 2997
AcsI	RAATTY	9 cut(s)	794, 1001, 1187, 1226, 1638, 1732, 1975, 2681, 3200
AcuI	CTGAAG	5 cut(s)	867, 2280, 2435, 2490, 2673
AcyI	GRCGYC	3 cut(s)	78, 90, 255
AfaI	GTAC	3 cut(s)	1135, 1461, 2074
AfiI	CCNNNNNNNGG	9 cut(s)	215, 550, 932, 1331, 2490, 2517, 2626, 2901, 3195
AflIII	ACRYGT	1 cut(s)	3069
AjiI	CACGTC	1 cut(s)	382
AjnI	CCWGG	6 cut(s)	208, 750, 1219, 2330, 2815, 3015
AjuI	GAANNNNNNNTTGG	2 cut(s)	89, 121
AleI	CACNNNNGTG	2 cut(s)	1163, 2167
Alw21I	GWGCWC	2 cut(s)	658, 2174
Alw26I	GTCTC	6 cut(s)	199, 246, 461, 988, 2136, 3357
Alw44I	GTGCAC	1 cut(s)	2170
AlwNI	CAGNNNCTG	1 cut(s)	1784
Ama87I	CYCGRG	1 cut(s)	2063
ApaLI	GTGCAC	1 cut(s)	2170
ApoI	RAATTY	9 cut(s)	794, 1001, 1187, 1226, 1638, 1732, 1975, 2681, 3200
ArsI	GACNNNNNNTTYG	2 cut(s)	309, 341
AseI	ATTAAT	2 cut(s)	1380, 1416
Asp700I	GAANNNNTTC	4 cut(s)	1230, 1575, 2385, 3204
Asp718I	GGTACC	1 cut(s)	1133
AspLEI	GCGC	4 cut(s)	61, 80, 1351, 2349
AspS9I	GGNCC	8 cut(s)	37, 430, 887, 1653, 1738, 2674, 2904, 3153
AsuC2I	CCSGG	1 cut(s)	2556
AsuHPI	GGTGA	6 cut(s)	100, 157, 1177, 1235, 2330, 2552
AsuII	TTCGAA	2 cut(s)	407, 2303
AvaI	CYCGRG	1 cut(s)	2063
AvaII	GGWCC	5 cut(s)	430, 887, 1653, 2674, 3153
AxyI	CCTNAGG	1 cut(s)	2925
BaeGI	GKGCMC	4 cut(s)	1711, 2084, 2174, 2410
BalI	TGGCCA	1 cut(s)	2999
BamHI	GGATCC	1 cut(s)	2610
BanI	GGYRCC	3 cut(s)	77, 1133, 2132
BanII	GRGCYC	1 cut(s)	2221
BarI	GAAGNNNNNNTAC	2 cut(s)	1560, 1592
BauI	CACGAG	1 cut(s)	1123
BbsI	GAAGAC	1 cut(s)	3063
Bbv12I	GWGCWC	2 cut(s)	658, 2174
BceAI	ACGGC	2 cut(s)	1608, 1881
BcgI	CGANNNNNNTGC	4 cut(s)	645, 679, 2705, 2739
BciT130I	CCWGG	6 cut(s)	210, 752, 1221, 2332, 2817, 3017
BciVI	GTATCC	3 cut(s)	1111, 2329, 3439
BclI	TGATCA	3 cut(s)	2029, 2200, 2778
BcnI	CCSGG	1 cut(s)	2556
BcoDI	GTCTC	6 cut(s)	199, 246, 461, 988, 2136, 3357
BfmI	CTRYAG	6 cut(s)	969, 1581, 1661, 2534, 2748, 3061
BfoI	RGCGCY	2 cut(s)	62, 81
BfuI	GTATCC	3 cut(s)	1111, 2329, 3439
BlpI	GCTNAGC	1 cut(s)	3108
Bme1390I	CCNGG	7 cut(s)	210, 752, 1221, 2332, 2556, 2817, 3017
Bme18I	GGWCC	5 cut(s)	430, 887, 1653, 2674, 3153
BmeT110I	CYCGRG	1 cut(s)	2063
BmgBI	CACGTC	1 cut(s)	382
BmgT120I	GGNCC	8 cut(s)	37, 430, 887, 1653, 1738, 2674, 2904, 3153
BmiI	GGNNCC	9 cut(s)	39, 79, 924, 1135, 1654, 2134, 2522, 2612, 2676
BmrFI	CCNGG	7 cut(s)	210, 752, 1221, 2332, 2556, 2817, 3017
BmrI	ACTGGG	3 cut(s)	1230, 2087, 2635
BmuI	ACTGGG	3 cut(s)	1230, 2087, 2635
BpiI	GAAGAC	1 cut(s)	3063
BplI	GAGNNNNNCTC	4 cut(s)	1194, 1226, 2793, 2825
BpmI	CTGGAG	2 cut(s)	2238, 3038
Bpu10I	CCTNAGC	1 cut(s)	2514
Bpu1102I	GCTNAGC	1 cut(s)	3108
Bpu14I	TTCGAA	2 cut(s)	407, 2303
BpuEI	CTTGAG	4 cut(s)	1130, 2122, 2375, 3200
BpuMI	CCSGG	1 cut(s)	2556
BsaBI	GATNNNNATC	3 cut(s)	1014, 1416, 2615
BsaHI	GRCGYC	3 cut(s)	78, 90, 255
BsaI	GGTCTC	2 cut(s)	199, 2136
BsaJI	CCNNGG	7 cut(s)	209, 1470, 1863, 2331, 2690, 2816, 3102
BsaWI	WCCGGW	1 cut(s)	2149
BsaXI	ACNNNNNCTCC	2 cut(s)	210, 240
Bsc4I	CCNNNNNNNGG	9 cut(s)	215, 550, 932, 1331, 2490, 2517, 2626, 2901, 3195
Bse118I	RCCGGY	3 cut(s)	257, 441, 2957
Bse21I	CCTNAGG	1 cut(s)	2925
Bse3DI	GCAATG	3 cut(s)	961, 2397, 3000
Bse8I	GATNNNNATC	3 cut(s)	1014, 1416, 2615
BseBI	CCWGG	6 cut(s)	210, 752, 1221, 2332, 2817, 3017
BseDI	CCNNGG	7 cut(s)	209, 1470, 1863, 2331, 2690, 2816, 3102
BseJI	GATNNNNATC	3 cut(s)	1014, 1416, 2615
BseLI	CCNNNNNNNGG	9 cut(s)	215, 550, 932, 1331, 2490, 2517, 2626, 2901, 3195
BseMI	GCAATG	3 cut(s)	961, 2397, 3000
BseRI	GAGGAG	2 cut(s)	264, 1127
BseSI	GKGCMC	4 cut(s)	1711, 2084, 2174, 2410
BseYI	CCCAGC	2 cut(s)	554, 2885
BsgI	GTGCAG	1 cut(s)	167
Bsh1236I	CGCG	4 cut(s)	452, 465, 488, 2975
BshNI	GGYRCC	3 cut(s)	77, 1133, 2132
BsiHKAI	GWGCWC	2 cut(s)	658, 2174
BsiHKCI	CYCGRG	1 cut(s)	2063
BsiSI	CCGG	6 cut(s)	75, 258, 442, 2150, 2556, 2958
BslFI	GGGAC	3 cut(s)	2506, 2660, 3419
BslI	CCNNNNNNNGG	9 cut(s)	215, 550, 932, 1331, 2490, 2517, 2626, 2901, 3195
BsmAI	GTCTC	6 cut(s)	199, 246, 461, 988, 2136, 3357
BsmBI	CGTCTC	2 cut(s)	246, 461
BsmFI	GGGAC	3 cut(s)	2506, 2660, 3419
BsmI	GAATGC	3 cut(s)	2272, 2317, 2349
Bso31I	GGTCTC	2 cut(s)	199, 2136
BsoBI	CYCGRG	1 cut(s)	2063
Bsp119I	TTCGAA	2 cut(s)	407, 2303
Bsp1286I	GDGCHC	6 cut(s)	658, 1711, 2084, 2174, 2221, 2410
Bsp1720I	GCTNAGC	1 cut(s)	3108
Bsp19I	CCATGG	2 cut(s)	1470, 3102
BspFNI	CGCG	4 cut(s)	452, 465, 488, 2975
BspLI	GGNNCC	9 cut(s)	39, 79, 924, 1135, 1654, 2134, 2522, 2612, 2676
BspMAI	CTGCAG	1 cut(s)	2538
BspQI	GCTCTTC	1 cut(s)	488
BspT104I	TTCGAA	2 cut(s)	407, 2303
BspT107I	GGYRCC	3 cut(s)	77, 1133, 2132
BspTNI	GGTCTC	2 cut(s)	199, 2136
BsrBI	CCGCTC	1 cut(s)	3363
BsrDI	GCAATG	3 cut(s)	961, 2397, 3000
BsrFI	RCCGGY	3 cut(s)	257, 441, 2957
BssAI	RCCGGY	3 cut(s)	257, 441, 2957
BssECI	CCNNGG	7 cut(s)	209, 1470, 1863, 2331, 2690, 2816, 3102
BssNI	GRCGYC	3 cut(s)	78, 90, 255
BssSI	CACGAG	1 cut(s)	1123
BssT1I	CCWWGG	3 cut(s)	1470, 2690, 3102
Bst2BI	CACGAG	1 cut(s)	1123
Bst2UI	CCWGG	6 cut(s)	210, 752, 1221, 2332, 2817, 3017
Bst4CI	ACNGT	8 cut(s)	188, 325, 362, 901, 1780, 2918, 3140, 3351
Bst6I	CTCTTC	5 cut(s)	488, 1335, 1799, 1835, 2300
BstACI	GRCGYC	3 cut(s)	78, 90, 255
BstAPI	GCANNNNNTGC	2 cut(s)	190, 2088
BstBI	TTCGAA	2 cut(s)	407, 2303
BstDSI	CCRYGG	3 cut(s)	1470, 1863, 3102
BstEII	GGTNACC	1 cut(s)	2558
BstFNI	CGCG	4 cut(s)	452, 465, 488, 2975
BstH2I	RGCGCY	2 cut(s)	62, 81
BstHHI	GCGC	4 cut(s)	61, 80, 1351, 2349
BstMAI	GTCTC	6 cut(s)	199, 246, 461, 988, 2136, 3357
BstNI	CCWGG	6 cut(s)	210, 752, 1221, 2332, 2817, 3017
BstPI	GGTNACC	1 cut(s)	2558
BstSCI	CCNGG	7 cut(s)	208, 750, 1219, 2330, 2554, 2815, 3015
BstSFI	CTRYAG	6 cut(s)	969, 1581, 1661, 2534, 2748, 3061
BstSLI	GKGCMC	4 cut(s)	1711, 2084, 2174, 2410
BstUI	CGCG	4 cut(s)	452, 465, 488, 2975
BstV2I	GAAGAC	1 cut(s)	3063
BstX2I	RGATCY	5 cut(s)	1015, 1700, 2610, 2875, 3319
BstXI	CCANNNNNNTGG	2 cut(s)	2995, 3007
BstYI	RGATCY	5 cut(s)	1015, 1700, 2610, 2875, 3319
Bsu36I	CCTNAGG	1 cut(s)	2925
BsuI	GTATCC	3 cut(s)	1111, 2329, 3439
BtgI	CCRYGG	3 cut(s)	1470, 1863, 3102
BtrI	CACGTC	1 cut(s)	382
BtsI	GCAGTG	3 cut(s)	402, 2203, 2531
CaiI	CAGNNNCTG	1 cut(s)	1784
CfoI	GCGC	4 cut(s)	61, 80, 1351, 2349
Cfr10I	RCCGGY	3 cut(s)	257, 441, 2957
Cfr13I	GGNCC	8 cut(s)	37, 430, 887, 1653, 1738, 2674, 2904, 3153
CseI	GACGC	4 cut(s)	98, 263, 477, 2330
Csp6I	GTAC	3 cut(s)	1134, 1460, 2073
CspCI	CAANNNNNGTGG	2 cut(s)	1111, 1146
CviQI	GTAC	3 cut(s)	1134, 1460, 2073
DinI	GGCGCC	1 cut(s)	79
EaeI	YGGCCR	4 cut(s)	301, 460, 1259, 2997
Eam1104I	CTCTTC	5 cut(s)	488, 1335, 1799, 1835, 2300
EarI	CTCTTC	5 cut(s)	488, 1335, 1799, 1835, 2300
EciI	GGCGGA	1 cut(s)	256
Eco130I	CCWWGG	3 cut(s)	1470, 2690, 3102
Eco147I	AGGCCT	1 cut(s)	858
Eco24I	GRGCYC	1 cut(s)	2221
Eco31I	GGTCTC	2 cut(s)	199, 2136
Eco32I	GATATC	1 cut(s)	421
Eco47I	GGWCC	5 cut(s)	430, 887, 1653, 2674, 3153
Eco57I	CTGAAG	5 cut(s)	867, 2280, 2435, 2490, 2673
Eco81I	CCTNAGG	1 cut(s)	2925
Eco88I	CYCGRG	1 cut(s)	2063
Eco91I	GGTNACC	1 cut(s)	2558
EcoO109I	RGGNCCY	1 cut(s)	430
EcoO65I	GGTNACC	1 cut(s)	2558
EcoRI	GAATTC	1 cut(s)	794
EcoRII	CCWGG	6 cut(s)	208, 750, 1219, 2330, 2815, 3015
EcoRV	GATATC	1 cut(s)	421
EcoT14I	CCWWGG	3 cut(s)	1470, 2690, 3102
EcoT38I	GRGCYC	1 cut(s)	2221
EgeI	GGCGCC	1 cut(s)	79
EheI	GGCGCC	1 cut(s)	79
ErhI	CCWWGG	3 cut(s)	1470, 2690, 3102
Esp3I	CGTCTC	2 cut(s)	246, 461
FalI	AAGNNNNNCTT	2 cut(s)	723, 755
FaqI	GGGAC	3 cut(s)	2506, 2660, 3419
FauI	CCCGC	1 cut(s)	2980
FauNDI	CATATG	2 cut(s)	2871, 3250
FbaI	TGATCA	3 cut(s)	2029, 2200, 2778
FriOI	GRGCYC	1 cut(s)	2221
FspI	TGCGCA	1 cut(s)	1350
GlaI	GCGC	4 cut(s)	60, 79, 1350, 2348
GsaI	CCCAGC	2 cut(s)	558, 2889
GsuI	CTGGAG	2 cut(s)	2238, 3038
HaeII	RGCGCY	2 cut(s)	62, 81
HapII	CCGG	6 cut(s)	75, 258, 442, 2150, 2556, 2958
HgaI	GACGC	4 cut(s)	98, 263, 477, 2330
HhaI	GCGC	4 cut(s)	61, 80, 1351, 2349
Hin1I	GRCGYC	3 cut(s)	78, 90, 255
Hin6I	GCGC	4 cut(s)	59, 78, 1349, 2347
HinP1I	GCGC	4 cut(s)	59, 78, 1349, 2347
HincII	GTYRAC	3 cut(s)	985, 2932, 3415
HindII	GTYRAC	3 cut(s)	985, 2932, 3415
HindIII	AAGCTT	2 cut(s)	1355, 1515
HpaII	CCGG	6 cut(s)	75, 258, 442, 2150, 2556, 2958
HphI	GGTGA	6 cut(s)	100, 157, 1177, 1235, 2330, 2552
Hpy166II	GTNNAC	6 cut(s)	897, 985, 2075, 2172, 2932, 3415
Hpy8I	GTNNAC	6 cut(s)	897, 985, 2075, 2172, 2932, 3415
Hpy99I	CGWCG	7 cut(s)	86, 89, 92, 283, 428, 493, 992
HpyAV	CCTTC	9 cut(s)	56, 443, 578, 844, 1068, 1975, 2842, 3118, 3395
HpyCH4III	ACNGT	8 cut(s)	188, 325, 362, 901, 1780, 2918, 3140, 3351
HpyCH4IV	ACGT	3 cut(s)	381, 2339, 3071
HpySE526I	ACGT	3 cut(s)	381, 2339, 3071
Hsp92I	GRCGYC	3 cut(s)	78, 90, 255
HspAI	GCGC	4 cut(s)	59, 78, 1349, 2347
KasI	GGCGCC	1 cut(s)	77
KpnI	GGTACC	1 cut(s)	1137
KroI	GCCGGC	3 cut(s)	257, 441, 2957
KroNI	GCCGGC	3 cut(s)	259, 443, 2959
Ksp22I	TGATCA	3 cut(s)	2029, 2200, 2778
LguI	GCTCTTC	1 cut(s)	488
LmnI	GCTCC	7 cut(s)	66, 129, 269, 595, 2344, 3019, 3368
MaeII	ACGT	3 cut(s)	381, 2339, 3071
MbiI	CCGCTC	1 cut(s)	3363
MflI	RGATCY	5 cut(s)	1015, 1700, 2610, 2875, 3319
MhlI	GDGCHC	6 cut(s)	658, 1711, 2084, 2174, 2221, 2410
MlsI	TGGCCA	1 cut(s)	2999
MluNI	TGGCCA	1 cut(s)	2999
Mly113I	GGCGCC	1 cut(s)	78
MlyI	GAGTC	4 cut(s)	228, 1556, 2075, 2488
MmeI	TCCRAC	3 cut(s)	1678, 2322, 2460
Mox20I	TGGCCA	1 cut(s)	2999
MroNI	GCCGGC	3 cut(s)	257, 441, 2957
MroXI	GAANNNNTTC	4 cut(s)	1230, 1575, 2385, 3204
MscI	TGGCCA	1 cut(s)	2999
MslI	CAYNNNNRTG	7 cut(s)	395, 1163, 1714, 2099, 2167, 2993, 3372
Msp20I	TGGCCA	1 cut(s)	2999
MspA1I	CMGCKG	3 cut(s)	1880, 2258, 2452
MspI	CCGG	6 cut(s)	75, 258, 442, 2150, 2556, 2958
MspR9I	CCNGG	7 cut(s)	210, 752, 1221, 2332, 2556, 2817, 3017
Mva1269I	GAATGC	3 cut(s)	2272, 2317, 2349
MvaI	CCWGG	6 cut(s)	210, 752, 1221, 2332, 2817, 3017
MvnI	CGCG	4 cut(s)	452, 465, 488, 2975
NaeI	GCCGGC	3 cut(s)	259, 443, 2959
NarI	GGCGCC	1 cut(s)	78
NciI	CCSGG	1 cut(s)	2556
NcoI	CCATGG	2 cut(s)	1470, 3102
NdeI	CATATG	2 cut(s)	2871, 3250
NgoMIV	GCCGGC	3 cut(s)	257, 441, 2957
NlaIV	GGNNCC	9 cut(s)	39, 79, 924, 1135, 1654, 2134, 2522, 2612, 2676
NmuCI	GTSAC	8 cut(s)	568, 878, 1156, 1780, 2318, 2558, 2710, 3140
NsbI	TGCGCA	1 cut(s)	1350
NspV	TTCGAA	2 cut(s)	407, 2303
OliI	CACNNNNGTG	2 cut(s)	1163, 2167
PaeR7I	CTCGAG	1 cut(s)	2063
PceI	AGGCCT	1 cut(s)	858
PciSI	GCTCTTC	1 cut(s)	488
PcsI	WCGNNNNNNNCGW	2 cut(s)	306, 2850
PctI	GAATGC	3 cut(s)	2272, 2317, 2349
PdiI	GCCGGC	3 cut(s)	259, 443, 2959
PdmI	GAANNNNTTC	4 cut(s)	1230, 1575, 2385, 3204
PfeI	GAWTC	7 cut(s)	317, 404, 511, 1320, 1465, 1571, 1595
PleI	GAGTC	4 cut(s)	227, 1556, 2074, 2488
PluTI	GGCGCC	1 cut(s)	81
PpsI	GAGTC	4 cut(s)	227, 1556, 2074, 2488
PpuMI	RGGWCCY	1 cut(s)	430
PshBI	ATTAAT	2 cut(s)	1380, 1416
PsiI	TTATAA	1 cut(s)	3270
Psp5II	RGGWCCY	1 cut(s)	430
Psp6I	CCWGG	6 cut(s)	208, 750, 1219, 2330, 2815, 3015
PspEI	GGTNACC	1 cut(s)	2558
PspFI	CCCAGC	2 cut(s)	554, 2885
PspGI	CCWGG	6 cut(s)	208, 750, 1219, 2330, 2815, 3015
PspN4I	GGNNCC	9 cut(s)	39, 79, 924, 1135, 1654, 2134, 2522, 2612, 2676
PspPI	GGNCC	8 cut(s)	37, 430, 887, 1653, 1738, 2674, 2904, 3153
PspPPI	RGGWCCY	1 cut(s)	430
PstI	CTGCAG	1 cut(s)	2538
PstNI	CAGNNNCTG	1 cut(s)	1784
PsuI	RGATCY	5 cut(s)	1015, 1700, 2610, 2875, 3319
PvuII	CAGCTG	2 cut(s)	2258, 2452
RsaI	GTAC	3 cut(s)	1135, 1461, 2074
RsaNI	GTAC	3 cut(s)	1134, 1460, 2073
RseI	CAYNNNNRTG	7 cut(s)	395, 1163, 1714, 2099, 2167, 2993, 3372
SapI	GCTCTTC	1 cut(s)	488
Sau96I	GGNCC	8 cut(s)	37, 430, 887, 1653, 1738, 2674, 2904, 3153
SchI	GAGTC	4 cut(s)	228, 1556, 2075, 2488
ScrFI	CCNGG	7 cut(s)	210, 752, 1221, 2332, 2556, 2817, 3017
SduI	GDGCHC	6 cut(s)	658, 1711, 2084, 2174, 2221, 2410
SfcI	CTRYAG	6 cut(s)	969, 1581, 1661, 2534, 2748, 3061
SfoI	GGCGCC	1 cut(s)	79
Sfr274I	CTCGAG	1 cut(s)	2063
SfuI	TTCGAA	2 cut(s)	407, 2303
SinI	GGWCC	5 cut(s)	430, 887, 1653, 2674, 3153
SlaI	CTCGAG	1 cut(s)	2063
SmiMI	CAYNNNNRTG	7 cut(s)	395, 1163, 1714, 2099, 2167, 2993, 3372
SmlI	CTYRAG	5 cut(s)	1109, 2063, 2137, 2354, 3215
SmoI	CTYRAG	5 cut(s)	1109, 2063, 2137, 2354, 3215
SseBI	AGGCCT	1 cut(s)	858
SspDI	GGCGCC	1 cut(s)	77
SspI	AATATT	1 cut(s)	1689
StuI	AGGCCT	1 cut(s)	858
StyD4I	CCNGG	7 cut(s)	208, 750, 1219, 2330, 2554, 2815, 3015
StyI	CCWWGG	3 cut(s)	1470, 2690, 3102
TaaI	ACNGT	8 cut(s)	188, 325, 362, 901, 1780, 2918, 3140, 3351
TaiI	ACGT	3 cut(s)	384, 2342, 3074
TaqII	GACCGA	2 cut(s)	2729, 3312
TauI	GCSGC	6 cut(s)	38, 95, 465, 478, 481, 1435
TfiI	GAWTC	7 cut(s)	317, 404, 511, 1320, 1465, 1571, 1595
TseFI	GTSAC	8 cut(s)	568, 878, 1156, 1780, 2318, 2558, 2710, 3140
Tsp45I	GTSAC	8 cut(s)	568, 878, 1156, 1780, 2318, 2558, 2710, 3140
TspDTI	ATGAA	7 cut(s)	597, 826, 1028, 1398, 1743, 2843, 3414
TspGWI	ACGGA	3 cut(s)	1823, 3054, 3431
VneI	GTGCAC	1 cut(s)	2170
VpaK11BI	GGWCC	5 cut(s)	430, 887, 1653, 2674, 3153
VspI	ATTAAT	2 cut(s)	1380, 1416
XapI	RAATTY	9 cut(s)	794, 1001, 1187, 1226, 1638, 1732, 1975, 2681, 3200
XcmI	CCANNNNNNNNNTGG	3 cut(s)	2338, 2622, 2992
XhoI	CTCGAG	1 cut(s)	2063
XmnI	GAANNNNTTC	4 cut(s)	1230, 1575, 2385, 3204

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.