RLG00000024878

Ubiquitin-activating enzyme E1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002217
Physical Location & Seq
Reverse (-)
39998035 .. 40004283
6249 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024878

Sequence Viewer

Length: 3240 bp
ATGCTTCCTAGAAAGAGACAAGTGGGCGGAGAGGTTGTAGTAGAAGAAGGGGAACCAGATTTCGCCAACGAATCTCCAATCAAGAAGCTTTGCACCGACGAATCTAAGGGCAGTGACTGCAAAAGAACCGGTAACAGCAATATCAGCAGCAGCAACAGTAGCAGCAGCGTCAAGCCGCCGATCATGGCACTAGGGAATGGGAATTCAGGGGATATTGATGAGGATCTCCACAGCCGGCAGCTTGCTGTGTATGGCCGTGAGACGATGAGGCGGCTCTTTGCCTCCAACATTCTTGTCTCTGGGATGCAAGGCCTTGGTGCCGAGATTGCAAAGAACCTTGTTCTTGCCGGTGTCAAGTCTGTGACCTTGCATGATGAAGGAGTTGTTGAGTTGTGGGACCTGTCTGGTAATTTCTTTTTCACCGAAGACGATATTGGAAAGAATCGGGCCCTTGCTTGTGTCCAAAAATTGCAAGAACTTAATAATGCTGTGCTCATTTCGACTTTGACAACTCAGCTGACTAAAGAAAAACTTTCTGATTTCCAGGCTGTAGTTTTCACTGATATCAGTTTAGACAAAGCAATTGAATTCGATGATTACTGCCATAATCATCAGCCTCCAATCTCTTTTATCAAAACTGAAGTACGGGGCCTTTTTGGCAGTGTGTTTTGTGACTTTGGTCCTGAGTTCACTGTTCTTGATGTTGATGGTGAGGATCCACACACAGGCATCGTTGCATCAATTAGCAATGACAACCCTGCTATGATATCATGTGTCGATGATGAGAGGCTTGAGTTTCAGGACGGGGATTTGGTTCTGTTCACTGAAGTCCATGGAATGACCGAGTTGAATGACGGAAAGCCGAGGAAGATTAAGAATGCAAGGCCGTATTCATTCACAATCGAGGAGGACACTACAAATTATGCAGGATATGAGAAAGGTGGTATAGTTACACAGGTTAAGCAACCCAAGGTGTTGAAATTTAAGCCCTTGCGAGAAGCAGTCAAGGAACCTGGTGATTTCCTTCTGATTGACTTCTCCAAGTTTGATCGGCCACCTCTCCTGCACTTGGCATTTCAAGCACTAGATAAGTTTATATCCGAGTTAGGACGCTTCCCTGTTGCTGGATCCGAGGATGATGCTACAAAGTTCATCTCATTGGTAGCTAGCATAAATGATAGCTCGGCAGATGGGAGGCTTGAGGAGATTGATCAGAAGATTCTTCGTCACTTTGCATTTGGTGCTAGGGCTGTGCTGAATCCCATGGCTGCAATGTTTGGTGGTATTGTTGGACAGGAGGTTGTGAAGGCCTGTTCTGCAAAGTTTCACCCTCTTTTCCAGTTTTTCTATTTCGATTCGGTTGAATCACTTCCAACAGAAGCCTTGGACCCTAGTGATTTGAAGCCTCTAAACAGTCGGTATGATGCACAAATTTCAGTGTTTGGAGCCAAGCTGCAGAAAAAGCTGGAGGATTCAAAAGTGTTCATTGTTGGGTCTGGTGCACTAGGATGCGAGTTTTTGAAGAATTTAGCTTTGATGGGCGTTGCTTGTGGTCAAGAAGGTAAATTAACAATTACAGATGATGATGTCATTGAGAAGAGTAACCTTAGTAGACAATTTCTCTTCCGGGATTGGAACATTGGGCAGGCTAAATCAACAGTTGCCGCCGCCGCCGCCGCACTTATAAACAGTCGTATCAATATTGAAGCACTGCAGAATCGTGCAAGCCCAGAGACTGAAAACGTGTTTGATGATACTTTCTGGGAGAATTTGGATGTTGTTATCAATGCTCTAGATAATGTGAACGCGAGGCTTTACATTGATCAGCGATGCTTGTATTTCCAGAAGCCACTTTTGGAATCAGGTACCCTCGGTGCCAAGTGCAACACACAGATGGTCATTCCTCACTTGACTGAAAATTATGGAGCATCACGGGACCCACCTGAAAAGCAAGCACCTATGTGCACTGTTCATTCATTTCCTCACAACATTGACCACTGCTTGACATGGGCTCGATCTGAGTTTGAGGGTTTACTTGAGAAGGTCCCAGCTGAAGTAAATGCCTACTTGACAAATCCTAGTGAGTACACTACTGCAATGAAGAATGCTGGTGATGCTCAGGCAAGGAATAACTTGGAAAGTGTCATTGAATGCCTTGACAAGGAGAGATGCGGGACATTTCAAGATTGCATAACCTGGGCTCGACTAAAGTTTGAGGACTACTTTTCCAACCGTGTGAAGCAGTTAACATATACTTTTCCGGAGGATGCTACAACCAGTAGTGGGACCCCATTCTGGTCTGCTCCCAAGCGTTTCCCTCGCCCACTGGTGTTCTCAGTAGATGATCTCAGCCACCTTCAATTTGTACTGGCAGCATCCATACTACGAGCTGAGACATTTAACATTGCAATTCCTGATTGGGTCAAGTCTACTCAAAAGTTTGCTGATGCTGTTAACAACGTGATGGTACCTGAATTTCAGCCTAAGAAGGATGTGAAGATTGTAACAGATGAGAAAGCTACAAACATTCTACCGGCATCCATTGATGATGCTGCTGTTATCAACGAGTTAGTTATGAAGTTAGAAAAATGCAGGGAGCAGCTCCCACCAGCCTTCAAGATGAACCCTATTCAATTTGAGAAGGATGATGACACGAACTATCATATGGATGTAATAGCTGGATTTGCAAATATGAGGGCAAGGAACTATAGTATCCCTGAAGTCGACAAGCTGAAGGCCAAATTCATTGCCGGTAGAATCATTCCTGCAATTGCAACATCTACTGCTCTTGCAACTGGTCTTGTCTGCTTGGAGCTGTACAAGGTTCTGGCCGGAGAGCACAAGATAGAGGACTACCGAAACACCTTTGCCAATCTTGCTCTTCCTCTGTTCTCCATGGCTGAACCAGTGCCTCCTAAAGTCATCAAGCACCAAGATATGAGCTGGACAGTTTGGGACAGATGGATTATAAAGGACAACCCAACTTTGAAGCAGCTCCTTAATTGGCTCAAAGAAAAGGGCTTAAACGCTTACAGCATCTCGTATGGCAGTTGCCTTCTGTATAACAGCATGTTCCCCAAGCACAGAGAGCGCATGGACAAGCATATGGTGGATTTGGCTAGGGATGTGGCAAAGGCAGAGCTGCCTCCATATCGGAACCACTTTGATGTGGTGGTGGCCTGCGAAGATGATGAGGATAATGATATTGACATCCCTCAGATCTCCATTTACTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko05012 map04120 map05012
Pfam Domains
Protein Families
E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1080

Amino Acids

120.47

Weight (kDa)

5.09

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 77 - 451 1.5e-24 ThiF family
E1_FCCH PF16190 251 - 321 4.8e-28 Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB PF16191 322 - 392 1.9e-24 Ubiquitin-activating enzyme E1 four-helix bundle
ThiF PF00899 474 - 966 1.5e-62 ThiF family
UBA_E1_SCCH PF10585 661 - 914 1.2e-86 Ubiquitin-activating enzyme, SCCH domain
E1_UFD PF09358 985 - 1074 6.3e-25 Ubiquitin fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1685, 2969
Acc65I GGTACC 2 cut(s) 1865, 2467
AccB1I GGYRCC 4 cut(s) 317, 1865, 1874, 2467
AccI GTMKAC 3 cut(s) 1612, 2429, 2724
AccII CGCG 1 cut(s) 1808
AccIII TCCGGA 1 cut(s) 2260
AciI CCGC 9 cut(s) 27, 176, 271, 1665, 1668, 1671, 1674, 1677, 2172
AclWI GGATC 5 cut(s) 231, 710, 723, 1122, 1135
AcoI YGGCCR 3 cut(s) 253, 1052, 2829
AcsI RAATTY 8 cut(s) 202, 587, 980, 1431, 1525, 1768, 2474, 2741
AcuI CTGAAG 5 cut(s) 660, 846, 2073, 2739, 2753
AfaI GTAC 6 cut(s) 645, 1867, 2087, 2367, 2469, 2819
AfiI CCNNNNNNNGG 6 cut(s) 725, 1069, 1124, 2161, 2283, 2295
AflIII ACRYGT 1 cut(s) 1743
AgeI ACCGGT 1 cut(s) 128
AjnI CCWGG 3 cut(s) 543, 1012, 2195
AjuI GAANNNNNNNTTGG 4 cut(s) 417, 449, 1838, 1870
AleI CACNNNNGTG 1 cut(s) 1960
Alw21I GWGCWC 4 cut(s) 495, 1504, 1967, 2841
Alw26I GTCTC 5 cut(s) 10, 254, 301, 1727, 2387
Alw44I GTGCAC 2 cut(s) 1500, 1963
AlwI GGATC 5 cut(s) 231, 710, 723, 1122, 1135
AlwNI CAGNNNCTG 2 cut(s) 117, 1736
Aor13HI TCCGGA 1 cut(s) 2260
ApaI GGGCCC 1 cut(s) 451
ApaLI GTGCAC 2 cut(s) 1500, 1963
ApoI RAATTY 8 cut(s) 202, 587, 980, 1431, 1525, 1768, 2474, 2741
AsiGI ACCGGT 1 cut(s) 128
Asp700I GAANNNNTTC 1 cut(s) 1368
Asp718I GGTACC 2 cut(s) 1865, 2467
AspLEI GCGC 1 cut(s) 3093
AspS9I GGNCC 9 cut(s) 397, 447, 448, 649, 680, 1387, 1936, 2044, 2286
AsuC2I CCSGG 1 cut(s) 1628
AsuHPI GGTGA 5 cut(s) 412, 722, 1028, 1319, 2123
AsuNHI GCTAGC 1 cut(s) 1166
AvaII GGWCC 6 cut(s) 397, 680, 1387, 1936, 2044, 2286
BaeGI GKGCMC 3 cut(s) 451, 1504, 1967
BaeI ACNNNNGTAYC 4 cut(s) 1678, 1711, 2695, 2728
BamHI GGATCC 2 cut(s) 715, 1127
BanI GGYRCC 4 cut(s) 317, 1865, 1874, 2467
BanII GRGCYC 3 cut(s) 451, 2014, 2203
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 4 cut(s) 495, 1504, 1967, 2841
BccI CCATC 6 cut(s) 701, 1184, 1531, 1888, 2458, 2955
BceAI ACGGC 2 cut(s) 240, 871
BcgI CGANNNNNNTGC 2 cut(s) 1121, 1155
BciT130I CCWGG 3 cut(s) 545, 1014, 2197
BciVI GTATCC 1 cut(s) 2723
BclI TGATCA 2 cut(s) 1210, 1822
BcnI CCSGG 1 cut(s) 1628
BcoDI GTCTC 5 cut(s) 10, 254, 301, 1727, 2387
BfmI CTRYAG 4 cut(s) 549, 1454, 1712, 2707
BfuI GTATCC 1 cut(s) 2723
BglI GCCNNNNNGGC 1 cut(s) 657
BglII AGATCT 1 cut(s) 3219
Bme1390I CCNGG 4 cut(s) 545, 1014, 1628, 2197
Bme18I GGWCC 6 cut(s) 397, 680, 1387, 1936, 2044, 2286
BmgT120I GGNCC 9 cut(s) 397, 447, 448, 649, 680, 1387, 1936, 2044, 2286
BmrFI CCNGG 4 cut(s) 545, 1014, 1628, 2197
BmtI GCTAGC 1 cut(s) 1170
BoxI GACNNNNGTC 1 cut(s) 678
BpiI GAAGAC 1 cut(s) 432
BpmI CTGGAG 1 cut(s) 1487
Bpu10I CCTNAGC 1 cut(s) 2118
BpuEI CTTGAG 3 cut(s) 812, 1220, 2057
BpuMI CCSGG 1 cut(s) 1628
BsaBI GATNNNNATC 1 cut(s) 222
BsaWI WCCGGW 2 cut(s) 128, 2260
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 6 cut(s) 725, 1069, 1124, 2161, 2283, 2295
Bse118I RCCGGY 5 cut(s) 128, 234, 347, 2533, 2750
Bse1I ACTGG 6 cut(s) 1339, 2277, 2331, 2373, 2800, 2906
Bse3DI GCAATG 5 cut(s) 754, 1278, 2103, 2403, 2745
Bse8I GATNNNNATC 1 cut(s) 222
BseAI TCCGGA 1 cut(s) 2260
BseBI CCWGG 3 cut(s) 545, 1014, 2197
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 6 cut(s) 725, 1069, 1124, 2161, 2283, 2295
BseMI GCAATG 5 cut(s) 754, 1278, 2103, 2403, 2745
BseMII CTCAG 8 cut(s) 527, 675, 2010, 2132, 2349, 2362, 2382, 3230
BseNI ACTGG 6 cut(s) 1339, 2277, 2331, 2373, 2800, 2906
BseRI GAGGAG 2 cut(s) 920, 1217
BseSI GKGCMC 3 cut(s) 451, 1504, 1967
BseYI CCCAGC 1 cut(s) 2047
BsgI GTGCAG 1 cut(s) 1049
Bsh1236I CGCG 1 cut(s) 1808
BshNI GGYRCC 4 cut(s) 317, 1865, 1874, 2467
BshTI ACCGGT 1 cut(s) 128
BsiHKAI GWGCWC 4 cut(s) 495, 1504, 1967, 2841
BsiSI CCGG 8 cut(s) 129, 235, 348, 1627, 2261, 2534, 2751, 2832
BslFI GGGAC 6 cut(s) 410, 1949, 2030, 2188, 2299, 2969
BslI CCNNNNNNNGG 6 cut(s) 725, 1069, 1124, 2161, 2283, 2295
BsmAI GTCTC 5 cut(s) 10, 254, 301, 1727, 2387
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 6 cut(s) 410, 1949, 2030, 2188, 2299, 2969
BsmI GAATGC 3 cut(s) 883, 2110, 2156
Bsp120I GGGCCC 1 cut(s) 447
Bsp1286I GDGCHC 7 cut(s) 451, 495, 1504, 1967, 2014, 2203, 2841
Bsp13I TCCGGA 1 cut(s) 2260
Bsp1407I TGTACA 1 cut(s) 2817
Bsp19I CCATGG 3 cut(s) 832, 1263, 2895
BspACI CCGC 9 cut(s) 27, 176, 271, 1665, 1668, 1671, 1674, 1677, 2172
BspCNI CTCAG 8 cut(s) 526, 676, 2011, 2131, 2348, 2361, 2383, 3229
BspEI TCCGGA 1 cut(s) 2260
BspFNI CGCG 1 cut(s) 1808
BspMAI CTGCAG 2 cut(s) 1458, 1716
BspOI GCTAGC 1 cut(s) 1170
BspPI GGATC 5 cut(s) 231, 710, 723, 1122, 1135
BspQI GCTCTTC 1 cut(s) 2886
BspT107I GGYRCC 4 cut(s) 317, 1865, 1874, 2467
BsrDI GCAATG 5 cut(s) 754, 1278, 2103, 2403, 2745
BsrFI RCCGGY 5 cut(s) 128, 234, 347, 2533, 2750
BsrGI TGTACA 1 cut(s) 2817
BsrI ACTGG 6 cut(s) 1339, 2277, 2331, 2373, 2800, 2906
BssAI RCCGGY 5 cut(s) 128, 234, 347, 2533, 2750
BssT1I CCWWGG 6 cut(s) 313, 832, 969, 1263, 1383, 2895
Bst2UI CCWGG 3 cut(s) 545, 1014, 2197
Bst4CI ACNGT 8 cut(s) 158, 694, 1415, 1660, 1691, 1969, 2234, 2950
Bst6I CTCTTC 3 cut(s) 1592, 1628, 2886
BstAPI GCANNNNNTGC 2 cut(s) 117, 1241
BstAUI TGTACA 1 cut(s) 2817
BstC8I GCNNGC 7 cut(s) 236, 243, 1168, 1647, 1726, 1953, 3181
BstDSI CCRYGG 3 cut(s) 832, 1263, 2895
BstENI CCTNNNNNAGG 1 cut(s) 2159
BstFNI CGCG 1 cut(s) 1808
BstHHI GCGC 1 cut(s) 3093
BstMAI GTCTC 5 cut(s) 10, 254, 301, 1727, 2387
BstNI CCWGG 3 cut(s) 545, 1014, 2197
BstNSI RCATGY 1 cut(s) 3073
BstPAI GACNNNNGTC 1 cut(s) 678
BstSCI CCNGG 4 cut(s) 543, 1012, 1626, 2195
BstSFI CTRYAG 4 cut(s) 549, 1454, 1712, 2707
BstSLI GKGCMC 3 cut(s) 451, 1504, 1967
BstUI CGCG 1 cut(s) 1808
BstV2I GAAGAC 1 cut(s) 432
BstX2I RGATCY 4 cut(s) 223, 715, 1127, 3219
BstYI RGATCY 4 cut(s) 223, 715, 1127, 3219
BsuI GTATCC 1 cut(s) 2723
BtgI CCRYGG 3 cut(s) 832, 1263, 2895
BtgZI GCGATG 1 cut(s) 1843
BtsI GCAGTG 4 cut(s) 118, 667, 1709, 1996
Cac8I GCNNGC 7 cut(s) 236, 243, 1168, 1647, 1726, 1953, 3181
CaiI CAGNNNCTG 2 cut(s) 117, 1736
CfoI GCGC 1 cut(s) 3093
Cfr10I RCCGGY 5 cut(s) 128, 234, 347, 2533, 2750
Cfr13I GGNCC 9 cut(s) 397, 447, 448, 649, 680, 1387, 1936, 2044, 2286
CseI GACGC 2 cut(s) 157, 1119
CsiI ACCWGGT 1 cut(s) 1012
Csp6I GTAC 6 cut(s) 644, 1866, 2086, 2366, 2468, 2818
CspAI ACCGGT 1 cut(s) 128
CviAII CATG 9 cut(s) 184, 371, 771, 833, 1264, 2007, 2896, 3070, 3094
CviQI GTAC 6 cut(s) 644, 1866, 2086, 2366, 2468, 2818
EaeI YGGCCR 3 cut(s) 253, 1052, 2829
Eam1104I CTCTTC 3 cut(s) 1592, 1628, 2886
EarI CTCTTC 3 cut(s) 1592, 1628, 2886
EciI GGCGGA 1 cut(s) 42
Eco130I CCWWGG 6 cut(s) 313, 832, 969, 1263, 1383, 2895
Eco147I AGGCCT 2 cut(s) 312, 1310
Eco24I GRGCYC 3 cut(s) 451, 2014, 2203
Eco32I GATATC 2 cut(s) 565, 768
Eco47I GGWCC 6 cut(s) 397, 680, 1387, 1936, 2044, 2286
Eco57I CTGAAG 5 cut(s) 660, 846, 2073, 2739, 2753
EcoNI CCTNNNNNAGG 1 cut(s) 2159
EcoO109I RGGNCCY 6 cut(s) 397, 448, 649, 1936, 2044, 2286
EcoRI GAATTC 2 cut(s) 202, 587
EcoRII CCWGG 3 cut(s) 543, 1012, 2195
EcoRV GATATC 2 cut(s) 565, 768
EcoT14I CCWWGG 6 cut(s) 313, 832, 969, 1263, 1383, 2895
EcoT38I GRGCYC 3 cut(s) 451, 2014, 2203
ErhI CCWWGG 6 cut(s) 313, 832, 969, 1263, 1383, 2895
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 9 cut(s) 187, 374, 774, 836, 1267, 2010, 2899, 3073, 3097
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 6 cut(s) 410, 1949, 2030, 2188, 2299, 2969
FatI CATG 9 cut(s) 183, 370, 770, 832, 1263, 2006, 2895, 3069, 3093
FauI CCCGC 1 cut(s) 2165
FauNDI CATATG 2 cut(s) 2664, 3105
FbaI TGATCA 2 cut(s) 1210, 1822
FblI GTMKAC 3 cut(s) 1612, 2429, 2724
FriOI GRGCYC 3 cut(s) 451, 2014, 2203
GlaI GCGC 1 cut(s) 3092
GsaI CCCAGC 1 cut(s) 2051
GsuI CTGGAG 1 cut(s) 1487
HapII CCGG 8 cut(s) 129, 235, 348, 1627, 2261, 2534, 2751, 2832
HgaI GACGC 2 cut(s) 157, 1119
HhaI GCGC 1 cut(s) 3093
Hin1II CATG 9 cut(s) 187, 374, 774, 836, 1267, 2010, 2899, 3073, 3097
Hin6I GCGC 1 cut(s) 3091
HinP1I GCGC 1 cut(s) 3091
HincII GTYRAC 3 cut(s) 2247, 2455, 2725
HindII GTYRAC 3 cut(s) 2247, 2455, 2725
HindIII AAGCTT 1 cut(s) 86
HpaI GTTAAC 2 cut(s) 2247, 2455
HpaII CCGG 8 cut(s) 129, 235, 348, 1627, 2261, 2534, 2751, 2832
HphI GGTGA 5 cut(s) 412, 722, 1028, 1319, 2123
Hpy188I TCNGA 8 cut(s) 538, 1029, 1102, 1132, 1215, 2020, 3156, 3219
Hpy99I CGWCG 1 cut(s) 101
HpyCH4III ACNGT 8 cut(s) 158, 694, 1415, 1660, 1691, 1969, 2234, 2950
HpyCH4IV ACGT 2 cut(s) 1743, 2460
HpySE526I ACGT 2 cut(s) 1743, 2460
Hsp92II CATG 9 cut(s) 187, 374, 774, 836, 1267, 2010, 2899, 3073, 3097
HspAI GCGC 1 cut(s) 3091
KflI GGGWCCC 2 cut(s) 1936, 2286
Kpn2I TCCGGA 1 cut(s) 2260
KpnI GGTACC 2 cut(s) 1869, 2471
KroI GCCGGC 1 cut(s) 234
KroNI GCCGGC 1 cut(s) 236
Ksp22I TGATCA 2 cut(s) 1210, 1822
KspAI GTTAAC 2 cut(s) 2247, 2455
LguI GCTCTTC 1 cut(s) 2886
LmnI GCTCC 7 cut(s) 1445, 1925, 2308, 2596, 2607, 2812, 3000
MabI ACCWGGT 1 cut(s) 1012
MaeII ACGT 2 cut(s) 1743, 2460
MaeIII GTNAC 8 cut(s) 113, 131, 361, 671, 949, 1226, 1601, 2503
MfeI CAATTG 2 cut(s) 582, 2769
MflI RGATCY 4 cut(s) 223, 715, 1127, 3219
MhlI GDGCHC 7 cut(s) 451, 495, 1504, 1967, 2014, 2203, 2841
MmeI TCCRAC 4 cut(s) 309, 1270, 1397, 2253
MroI TCCGGA 1 cut(s) 2260
MroNI GCCGGC 1 cut(s) 234
MroXI GAANNNNTTC 1 cut(s) 1368
MslI CAYNNNNRTG 5 cut(s) 1507, 1892, 1960, 2667, 3165
MspA1I CMGCKG 2 cut(s) 517, 2051
MspI CCGG 8 cut(s) 129, 235, 348, 1627, 2261, 2534, 2751, 2832
MspR9I CCNGG 4 cut(s) 545, 1014, 1628, 2197
MunI CAATTG 2 cut(s) 582, 2769
Mva1269I GAATGC 3 cut(s) 883, 2110, 2156
MvaI CCWGG 3 cut(s) 545, 1014, 2197
MvnI CGCG 1 cut(s) 1808
NaeI GCCGGC 1 cut(s) 236
NciI CCSGG 1 cut(s) 1628
NcoI CCATGG 3 cut(s) 832, 1263, 2895
NdeI CATATG 2 cut(s) 2664, 3105
NgoMIV GCCGGC 1 cut(s) 234
NheI GCTAGC 1 cut(s) 1166
NlaIII CATG 9 cut(s) 187, 374, 774, 836, 1267, 2010, 2899, 3073, 3097
NmeAIII GCCGAG 3 cut(s) 346, 888, 1163
NmuCI GTSAC 4 cut(s) 113, 361, 671, 1226
NspI RCATGY 1 cut(s) 3073
OliI CACNNNNGTG 1 cut(s) 1960
PceI AGGCCT 2 cut(s) 312, 1310
PciSI GCTCTTC 1 cut(s) 2886
PctI GAATGC 3 cut(s) 883, 2110, 2156
PdiI GCCGGC 1 cut(s) 236
PdmI GAANNNNTTC 1 cut(s) 1368
PfoI TCCNGGA 1 cut(s) 1626
PinAI ACCGGT 1 cut(s) 128
PpuMI RGGWCCY 4 cut(s) 397, 1936, 2044, 2286
PshAI GACNNNNGTC 1 cut(s) 678
PsiI TTATAA 2 cut(s) 1685, 2969
Psp5II RGGWCCY 4 cut(s) 397, 1936, 2044, 2286
Psp6I CCWGG 3 cut(s) 543, 1012, 2195
PspFI CCCAGC 1 cut(s) 2047
PspGI CCWGG 3 cut(s) 543, 1012, 2195
PspOMI GGGCCC 1 cut(s) 447
PspPI GGNCC 9 cut(s) 397, 447, 448, 649, 680, 1387, 1936, 2044, 2286
PspPPI RGGWCCY 4 cut(s) 397, 1936, 2044, 2286
PstI CTGCAG 2 cut(s) 1458, 1716
PstNI CAGNNNCTG 2 cut(s) 117, 1736
PsuI RGATCY 4 cut(s) 223, 715, 1127, 3219
PvuII CAGCTG 2 cut(s) 517, 2051
RsaI GTAC 6 cut(s) 645, 1867, 2087, 2367, 2469, 2819
RsaNI GTAC 6 cut(s) 644, 1866, 2086, 2366, 2468, 2818
RseI CAYNNNNRTG 5 cut(s) 1507, 1892, 1960, 2667, 3165
SalI GTCGAC 1 cut(s) 2723
SapI GCTCTTC 1 cut(s) 2886
Sau96I GGNCC 9 cut(s) 397, 447, 448, 649, 680, 1387, 1936, 2044, 2286
ScrFI CCNGG 4 cut(s) 545, 1014, 1628, 2197
SduI GDGCHC 7 cut(s) 451, 495, 1504, 1967, 2014, 2203, 2841
SexAI ACCWGGT 1 cut(s) 1012
SfcI CTRYAG 4 cut(s) 549, 1454, 1712, 2707
SinI GGWCC 6 cut(s) 397, 680, 1387, 1936, 2044, 2286
SmiMI CAYNNNNRTG 5 cut(s) 1507, 1892, 1960, 2667, 3165
SmlI CTYRAG 3 cut(s) 791, 1199, 2036
SmoI CTYRAG 3 cut(s) 791, 1199, 2036
SseBI AGGCCT 2 cut(s) 312, 1310
SsiI CCGC 9 cut(s) 27, 176, 271, 1665, 1668, 1671, 1674, 1677, 2172
SspI AATATT 1 cut(s) 1702
StuI AGGCCT 2 cut(s) 312, 1310
StyD4I CCNGG 4 cut(s) 543, 1012, 1626, 2195
StyI CCWWGG 6 cut(s) 313, 832, 969, 1263, 1383, 2895
TaaI ACNGT 8 cut(s) 158, 694, 1415, 1660, 1691, 1969, 2234, 2950
TaiI ACGT 2 cut(s) 1746, 2463
TaqI TCGA 8 cut(s) 500, 591, 777, 903, 1353, 2014, 2203, 2724
TaqII GACCGA 1 cut(s) 857
TatI WGTACW 3 cut(s) 2085, 2365, 2817
TauI GCSGC 7 cut(s) 178, 274, 1667, 1670, 1673, 1676, 1679
TseFI GTSAC 4 cut(s) 113, 361, 671, 1226
Tsp45I GTSAC 4 cut(s) 113, 361, 671, 1226
TspGWI ACGGA 1 cut(s) 870
VneI GTGCAC 2 cut(s) 1500, 1963
VpaK11BI GGWCC 6 cut(s) 397, 680, 1387, 1936, 2044, 2286
XagI CCTNNNNNAGG 1 cut(s) 2159
XapI RAATTY 8 cut(s) 202, 587, 980, 1431, 1525, 1768, 2474, 2741
XbaI TCTAGA 1 cut(s) 1792
XceI RCATGY 1 cut(s) 3073
XmiI GTMKAC 3 cut(s) 1612, 2429, 2724
XmnI GAANNNNTTC 1 cut(s) 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.