RH3CG119500

Ubiquitin-activating enzyme E1

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0002217
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG119500.1

Sequence Viewer

Length: 3057 bp
ATGGCGTTAGGAAATGGGAATTCGGGGGATATTGATGAGGATCTCCACAGCCGGCAGCTTGCTGTGTACGGCCGTGAGACGATGAGGCGGCTCTTTGCCTCCAACATTCTTGTCTCTGGGATGCAAGGCCTTGGTGCCGAGATTGCAAAGAACCTTGTTCTTGCCGGTGTCAAGTCTGTGACCTTGCATGATGAAGGAGTTGTTGAGTTGTGGGACCTGTCTGGTAATTTCTTTTTCACTGAAGACGATATTGGAAAGAATCGGGCCCTTGCTTGTGTCCAAAAATTGCAAGAACTTAATAATGCTGTGCTCATTTCAACTTTGACAACTCAGCTGACTAAAGAAAAACTTTCTGATTTCCAGGCTGTAGTTTTCACTGATATCAGCTTAGACAAAGCAATTGAATTCGATGATTACTGCCATAATCATCAGCCTCCAATCTCTTTTATCAAAACTGAAGTACGGGGCCTTTTTGGCAGTGTGTTTTGTGACTTTGGTCCTGAGTTCACTGTTCTTGATGTTGATGGTGAGGATCCACACACAGGCATAGTCGCATCAATTAGCAATGACAACCCTGCTATGATATCATGTGTCGATGATGAGAGGCTTGAGTTTCAGGACGGGGATTTGGTTCTGTTCACTGAAGTCCATGGAATGACCGAGTTGAATGACGGAAAGCCGAGGAAGATTAAGAATGCAAGGCCGTATTCATTCACAATTGAGCAGGACACTACAAATTATGCAGGATATGAGAAAGGTGGTATAGTTACACAGGTTAAGCAACCCAAGGTGTTGAAATTTAAGCCCTTGCGAGAAGCAGTCAAGGAACCTGGTGATTTCCTTCTGATTGACTTCTCCAAGTTTGATCGGCCACCTCTCCTGCACTTGGCATTTCAGGCACTAGATAAGTTTATATCAGAGTTAGGACGTTTCCCTGTTGCTGGATCCGAGGATGATGCTACAAAGTTCATATCATTGGTAACTAGCATAAATGATGGCTCGGCAGATGGGAGACTTGAGGAGATTGATCAGAAGATTCTTCGTCACTTTGCATTTGGTGCTAGGGCTGTGCTGAATCCCATGGCTGCAATGTTTGGTGGTATTGTTGGACAGGAGGTTGTGAAGGCCTGTTCTGCAAAGTTTCACCCTCTTTTCCAGTTTTTCTATTTCGATTCAGTTGAATCACTTCCGACAGAAGCCTTGGACCCTAGTGATTTGAAGCCTCTAAACAGTCGGTATGATGCACAAATTTCAGTATTTGGAGCCAAGCTGCAGCAAAAGCTGGAGGATTCAAAAGTGTTCACTGTTGGGTCTGGTGCACTAGGATGCGAGTTTTTGAAGAATTTAGCTTTGATGGGCGTTGCTTGTGGTCAAGAAGGTAAATTAACAATTACAGATGATGATGTCATTGAGAAGAGTAACCTTAGTAGACAATTTCTCTTCCGGGATTGGAACATTGGGCAGGCTAAATCAACAGTTGCTGCCACTGCTGCTGCACTTATAAACAGTCGTATCAATATTGAAGCACTGCAGAATCGTGCAAGCCCAGAGACTGAAAACGTGTTTGATGATACTTTCTGGGAGAATTTGGATGTTGTTATCAATGCTCTAGATAATGTGAATGCTAGGCTTTACATTGATCAGCGATGCTTGTATTTCCAGAAGCCACTTTTGGAATCAGGTACCCTAGGTGCCAAGTGCAACACACAGATGGTCATTCCTCACTTGACTGAAAATTATGGAGCATCACGGGACCCACCTGAAAAGCAAGCACCTATGTGCACAGTTCATTCATTTCCTCACAACATTGACCACTGCTTGACATGGGCTCGTTCTGAGTTTGAGGGTTTACTTGAGAAGGTCCCAGCTGAAGTAAATGCCTACTTGACAAATCCTAGTGAGTACACTACTGCAATGAAGAATGCTGGTGATGCTCAGGCAAGGAATAACTTGGAAAGTGTCATTGAATGCCTTGACAAGGAGAGATGCGAGACATTTCAAGATTGCATAACCTGGGCCCGACTAAAGTTTGAGGACTACTTTTCCAACCGTGTGAAGCAGTTAACATATACTTTTCCTGAGGATGCTACAACCAGTAGTGGGACCCCATTCTGGTCTGCTCCCAAGCGTTTCCCTCGCCCACTGGTGTTCTCAGTAGATGATCTCAGCCACCTTCAATTTGTACTGGCAGCATCCATACTACGAGCTGAGACATTTAACATTGCAATTCCTGATTGGATCAAGTCTACTCAAAAGTTTGCTGATGCTGTTAACAACGTGATGGTACCTGAATTTCAGCCTAAGAAGGATGTGAAGATTGTAATAGATGAGAAAGCTACAAACATTCTACCGGCATCCATCGATGATGCTGCTGTTATCAACGAGTTAGTTATGAAGTTAGAAAAATGCAGGGAGCAGCTCCCACCAGCCTTCAAGATGAACCCTATTCAATTTGAGAAGGATGATGACACAAACTATCATATGGATGTAATAGCTGGATTTGCAAATATGAGGGCTAGGAACTATAGTATCCCTGAAGTCGACAAGCTGAAGGCCAAATTCATCGCCGGTAGAATCATTCCTGCAATTGCAACATCCACTGCTCTTGCAACTGGTCTTGTCTGCTTGGAGCTGTACAAGGTTCTGGCCGGAGAGCACAAGATAGAGGACTACCGAAACACCTTTGCCAATCTTGCTCTTCCTCTGTTCTCCATGGCTGAACCAGTACCTCCTAAAGTCATCAAGCACCAAGATATGAGCTGGACAGTTTGGGACAGATGGATTATAAAGGACAACCCAACTTTGAAGCAGCTCCTTAATTGGCTCAAAGAAAAGGGCTTAAACGCTTACAGCATCTCTTATGGCAGTTGCCTTCTGTATAACAGCATGTTCCCCAAGCACAGAGAGCGCATGGACAAGCATATGGTGGATTTGGCTAGGGATGTGGCAAAGGCGGAGCTGCCTCCATATCGGAACCACTTTGATGTGGTGGTGGCCTGCGAAGATGATGAGGATAATGATATTGACATCCCTCAGATCTCCATTTACTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko05012 map04120 map05012
Pfam Domains
Protein Families
E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1018

Amino Acids

114.09

Weight (kDa)

5.04

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 16 - 390 1.3e-24 ThiF family
E1_FCCH PF16190 190 - 260 4.5e-28 Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB PF16191 261 - 331 1.1e-24 Ubiquitin-activating enzyme E1 four-helix bundle
ThiF PF00899 413 - 905 3.3e-61 ThiF family
UBA_E1_SCCH PF10585 600 - 853 1.6e-86 Ubiquitin-activating enzyme, SCCH domain
E1_UFD PF09358 924 - 1013 5.8e-25 Ubiquitin fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1502, 2786
Acc65I GGTACC 2 cut(s) 1682, 2284
AccB1I GGYRCC 4 cut(s) 134, 1682, 1691, 2284
AccI GTMKAC 3 cut(s) 1429, 2246, 2541
AciI CCGC 2 cut(s) 88, 2954
AclWI GGATC 6 cut(s) 48, 527, 540, 939, 952, 2246
AcoI YGGCCR 3 cut(s) 70, 869, 2646
AcsI RAATTY 8 cut(s) 19, 404, 797, 1248, 1342, 1585, 2291, 2558
AcuI CTGAAG 6 cut(s) 261, 477, 663, 1890, 2556, 2570
AfaI GTAC 8 cut(s) 68, 462, 1684, 1904, 2184, 2286, 2636, 2727
AfiI CCNNNNNNNGG 6 cut(s) 542, 886, 941, 1978, 2100, 2112
AflIII ACRYGT 1 cut(s) 1560
AjnI CCWGG 3 cut(s) 360, 829, 2012
AjuI GAANNNNNNNTTGG 4 cut(s) 234, 266, 1655, 1687
AleI CACNNNNGTG 1 cut(s) 1777
Alw21I GWGCWC 4 cut(s) 312, 1321, 1784, 2658
Alw26I GTCTC 6 cut(s) 71, 118, 1006, 1544, 1985, 2204
Alw44I GTGCAC 2 cut(s) 1317, 1780
AlwI GGATC 6 cut(s) 48, 527, 540, 939, 952, 2246
AlwNI CAGNNNCTG 2 cut(s) 1481, 1553
ApaI GGGCCC 2 cut(s) 268, 2020
ApaLI GTGCAC 2 cut(s) 1317, 1780
ApoI RAATTY 8 cut(s) 19, 404, 797, 1248, 1342, 1585, 2291, 2558
Asp700I GAANNNNTTC 1 cut(s) 1185
Asp718I GGTACC 2 cut(s) 1682, 2284
AspA2I CCTAGG 1 cut(s) 1687
AspLEI GCGC 1 cut(s) 2910
AsuC2I CCSGG 1 cut(s) 1445
AsuHPI GGTGA 4 cut(s) 539, 845, 1136, 1940
AvaII GGWCC 6 cut(s) 214, 497, 1204, 1753, 1861, 2103
AvrII CCTAGG 1 cut(s) 1687
AxyI CCTNAGG 1 cut(s) 2079
BaeGI GKGCMC 4 cut(s) 268, 1321, 1784, 2020
BaeI ACNNNNGTAYC 4 cut(s) 1495, 1528, 2512, 2545
BamHI GGATCC 2 cut(s) 532, 944
BanI GGYRCC 4 cut(s) 134, 1682, 1691, 2284
BanII GRGCYC 3 cut(s) 268, 1831, 2020
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 4 cut(s) 312, 1321, 1784, 2658
BccI CCATC 8 cut(s) 518, 989, 1001, 1348, 1705, 2275, 2366, 2772
BceAI ACGGC 3 cut(s) 57, 85, 688
BcgI CGANNNNNNTGC 4 cut(s) 938, 972, 2351, 2385
BciT130I CCWGG 3 cut(s) 362, 831, 2014
BciVI GTATCC 1 cut(s) 2540
BclI TGATCA 2 cut(s) 1027, 1639
BcnI CCSGG 1 cut(s) 1445
BcoDI GTCTC 6 cut(s) 71, 118, 1006, 1544, 1985, 2204
BfmI CTRYAG 4 cut(s) 366, 1271, 1529, 2524
BfuI GTATCC 1 cut(s) 2540
BglI GCCNNNNNGGC 1 cut(s) 474
BglII AGATCT 1 cut(s) 3036
BlnI CCTAGG 1 cut(s) 1687
Bme1390I CCNGG 4 cut(s) 362, 831, 1445, 2014
Bme18I GGWCC 6 cut(s) 214, 497, 1204, 1753, 1861, 2103
BmrFI CCNGG 4 cut(s) 362, 831, 1445, 2014
BoxI GACNNNNGTC 1 cut(s) 495
BpiI GAAGAC 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 1304
Bpu10I CCTNAGC 1 cut(s) 1935
BpuEI CTTGAG 3 cut(s) 629, 1037, 1874
BpuMI CCSGG 1 cut(s) 1445
Bsa29I ATCGAT 1 cut(s) 2361
BsaBI GATNNNNATC 1 cut(s) 39
Bsc4I CCNNNNNNNGG 6 cut(s) 542, 886, 941, 1978, 2100, 2112
Bse118I RCCGGY 4 cut(s) 51, 164, 2350, 2567
Bse1I ACTGG 6 cut(s) 1156, 2094, 2148, 2190, 2617, 2723
Bse21I CCTNAGG 1 cut(s) 2079
Bse3DI GCAATG 4 cut(s) 571, 1095, 1920, 2220
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 3 cut(s) 362, 831, 2014
BseCI ATCGAT 1 cut(s) 2361
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 6 cut(s) 542, 886, 941, 1978, 2100, 2112
BseMI GCAATG 4 cut(s) 571, 1095, 1920, 2220
BseMII CTCAG 9 cut(s) 344, 492, 1827, 1949, 2070, 2166, 2179, 2199, 3047
BseNI ACTGG 6 cut(s) 1156, 2094, 2148, 2190, 2617, 2723
BseRI GAGGAG 1 cut(s) 1034
BseSI GKGCMC 4 cut(s) 268, 1321, 1784, 2020
BseX3I CGGCCG 1 cut(s) 70
BseYI CCCAGC 1 cut(s) 1864
BsgI GTGCAG 2 cut(s) 866, 1479
Bsh1285I CGRYCG 1 cut(s) 73
BshNI GGYRCC 4 cut(s) 134, 1682, 1691, 2284
BshVI ATCGAT 1 cut(s) 2361
BsiEI CGRYCG 1 cut(s) 73
BsiHKAI GWGCWC 4 cut(s) 312, 1321, 1784, 2658
BsiSI CCGG 6 cut(s) 52, 165, 1444, 2351, 2568, 2649
BslFI GGGAC 5 cut(s) 227, 1766, 1847, 2116, 2786
BslI CCNNNNNNNGG 6 cut(s) 542, 886, 941, 1978, 2100, 2112
BsmAI GTCTC 6 cut(s) 71, 118, 1006, 1544, 1985, 2204
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 5 cut(s) 227, 1766, 1847, 2116, 2786
BsmI GAATGC 4 cut(s) 700, 1627, 1927, 1973
Bsp120I GGGCCC 2 cut(s) 264, 2016
Bsp1286I GDGCHC 7 cut(s) 268, 312, 1321, 1784, 1831, 2020, 2658
Bsp1407I TGTACA 1 cut(s) 2634
Bsp143I GATC 9 cut(s) 40, 532, 865, 944, 1027, 1639, 2161, 2238, 3036
Bsp19I CCATGG 3 cut(s) 649, 1080, 2712
BspACI CCGC 2 cut(s) 88, 2954
BspCNI CTCAG 9 cut(s) 343, 493, 1828, 1948, 2071, 2165, 2178, 2200, 3046
BspDI ATCGAT 1 cut(s) 2361
BspMAI CTGCAG 2 cut(s) 1275, 1533
BspPI GGATC 6 cut(s) 48, 527, 540, 939, 952, 2246
BspQI GCTCTTC 1 cut(s) 2703
BspT107I GGYRCC 4 cut(s) 134, 1682, 1691, 2284
BsrDI GCAATG 4 cut(s) 571, 1095, 1920, 2220
BsrFI RCCGGY 4 cut(s) 51, 164, 2350, 2567
BsrGI TGTACA 1 cut(s) 2634
BsrI ACTGG 6 cut(s) 1156, 2094, 2148, 2190, 2617, 2723
BssAI RCCGGY 4 cut(s) 51, 164, 2350, 2567
BssMI GATC 9 cut(s) 40, 532, 865, 944, 1027, 1639, 2161, 2238, 3036
BssT1I CCWWGG 7 cut(s) 130, 649, 786, 1080, 1200, 1687, 2712
Bst2UI CCWGG 3 cut(s) 362, 831, 2014
Bst4CI ACNGT 8 cut(s) 511, 1232, 1306, 1477, 1508, 1786, 2051, 2767
Bst6I CTCTTC 3 cut(s) 1409, 1445, 2703
BstAPI GCANNNNNTGC 1 cut(s) 1058
BstAUI TGTACA 1 cut(s) 2634
BstC8I GCNNGC 6 cut(s) 53, 60, 1464, 1543, 1770, 2998
BstDSI CCRYGG 3 cut(s) 649, 1080, 2712
BstENI CCTNNNNNAGG 1 cut(s) 1976
BstHHI GCGC 1 cut(s) 2910
BstKTI GATC 9 cut(s) 43, 535, 868, 947, 1030, 1642, 2164, 2241, 3039
BstMAI GTCTC 6 cut(s) 71, 118, 1006, 1544, 1985, 2204
BstMBI GATC 9 cut(s) 40, 532, 865, 944, 1027, 1639, 2161, 2238, 3036
BstMCI CGRYCG 1 cut(s) 73
BstNI CCWGG 3 cut(s) 362, 831, 2014
BstNSI RCATGY 1 cut(s) 2890
BstPAI GACNNNNGTC 1 cut(s) 495
BstSCI CCNGG 4 cut(s) 360, 829, 1443, 2012
BstSFI CTRYAG 4 cut(s) 366, 1271, 1529, 2524
BstSLI GKGCMC 4 cut(s) 268, 1321, 1784, 2020
BstV2I GAAGAC 1 cut(s) 249
BstX2I RGATCY 4 cut(s) 40, 532, 944, 3036
BstYI RGATCY 4 cut(s) 40, 532, 944, 3036
BstZI CGGCCG 1 cut(s) 70
Bsu15I ATCGAT 1 cut(s) 2361
Bsu36I CCTNAGG 1 cut(s) 2079
BsuI GTATCC 1 cut(s) 2540
BsuTUI ATCGAT 1 cut(s) 2361
BtgI CCRYGG 3 cut(s) 649, 1080, 2712
BtgZI GCGATG 2 cut(s) 1660, 2548
BtsI GCAGTG 5 cut(s) 484, 1485, 1526, 1813, 2598
Cac8I GCNNGC 6 cut(s) 53, 60, 1464, 1543, 1770, 2998
CaiI CAGNNNCTG 2 cut(s) 1481, 1553
CfoI GCGC 1 cut(s) 2910
Cfr10I RCCGGY 4 cut(s) 51, 164, 2350, 2567
ClaI ATCGAT 1 cut(s) 2361
CsiI ACCWGGT 1 cut(s) 829
Csp6I GTAC 8 cut(s) 67, 461, 1683, 1903, 2183, 2285, 2635, 2726
CspCI CAANNNNNGTGG 2 cut(s) 2587, 2622
CviAII CATG 8 cut(s) 188, 588, 650, 1081, 1824, 2713, 2887, 2911
CviQI GTAC 8 cut(s) 67, 461, 1683, 1903, 2183, 2285, 2635, 2726
DpnI GATC 9 cut(s) 42, 534, 867, 946, 1029, 1641, 2163, 2240, 3038
DpnII GATC 9 cut(s) 40, 532, 865, 944, 1027, 1639, 2161, 2238, 3036
EaeI YGGCCR 3 cut(s) 70, 869, 2646
EagI CGGCCG 1 cut(s) 70
Eam1104I CTCTTC 3 cut(s) 1409, 1445, 2703
EarI CTCTTC 3 cut(s) 1409, 1445, 2703
EciI GGCGGA 1 cut(s) 2969
EclXI CGGCCG 1 cut(s) 70
Eco130I CCWWGG 7 cut(s) 130, 649, 786, 1080, 1200, 1687, 2712
Eco147I AGGCCT 2 cut(s) 129, 1127
Eco24I GRGCYC 3 cut(s) 268, 1831, 2020
Eco32I GATATC 2 cut(s) 382, 585
Eco47I GGWCC 6 cut(s) 214, 497, 1204, 1753, 1861, 2103
Eco52I CGGCCG 1 cut(s) 70
Eco57I CTGAAG 6 cut(s) 261, 477, 663, 1890, 2556, 2570
Eco81I CCTNAGG 1 cut(s) 2079
EcoNI CCTNNNNNAGG 1 cut(s) 1976
EcoO109I RGGNCCY 6 cut(s) 214, 265, 466, 1753, 1861, 2103
EcoRI GAATTC 2 cut(s) 19, 404
EcoRII CCWGG 3 cut(s) 360, 829, 2012
EcoRV GATATC 2 cut(s) 382, 585
EcoT14I CCWWGG 7 cut(s) 130, 649, 786, 1080, 1200, 1687, 2712
EcoT38I GRGCYC 3 cut(s) 268, 1831, 2020
ErhI CCWWGG 7 cut(s) 130, 649, 786, 1080, 1200, 1687, 2712
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 8 cut(s) 191, 591, 653, 1084, 1827, 2716, 2890, 2914
FalI AAGNNNNNCTT 2 cut(s) 333, 365
FaqI GGGAC 5 cut(s) 227, 1766, 1847, 2116, 2786
FatI CATG 8 cut(s) 187, 587, 649, 1080, 1823, 2712, 2886, 2910
FauNDI CATATG 2 cut(s) 2481, 2922
FbaI TGATCA 2 cut(s) 1027, 1639
FblI GTMKAC 3 cut(s) 1429, 2246, 2541
FriOI GRGCYC 3 cut(s) 268, 1831, 2020
GlaI GCGC 1 cut(s) 2909
GsaI CCCAGC 1 cut(s) 1868
GsuI CTGGAG 1 cut(s) 1304
HapII CCGG 6 cut(s) 52, 165, 1444, 2351, 2568, 2649
HhaI GCGC 1 cut(s) 2910
Hin1II CATG 8 cut(s) 191, 591, 653, 1084, 1827, 2716, 2890, 2914
Hin6I GCGC 1 cut(s) 2908
HinP1I GCGC 1 cut(s) 2908
HincII GTYRAC 3 cut(s) 2064, 2272, 2542
HindII GTYRAC 3 cut(s) 2064, 2272, 2542
HinfI GANTC 9 cut(s) 259, 1036, 1075, 1172, 1181, 1289, 1534, 1676, 2574
HpaI GTTAAC 2 cut(s) 2064, 2272
HpaII CCGG 6 cut(s) 52, 165, 1444, 2351, 2568, 2649
HphI GGTGA 4 cut(s) 539, 845, 1136, 1940
Hpy188I TCNGA 9 cut(s) 355, 846, 919, 949, 1032, 1191, 1837, 2973, 3036
HpyCH4III ACNGT 8 cut(s) 511, 1232, 1306, 1477, 1508, 1786, 2051, 2767
HpyCH4IV ACGT 3 cut(s) 928, 1560, 2277
HpySE526I ACGT 3 cut(s) 928, 1560, 2277
Hsp92II CATG 8 cut(s) 191, 591, 653, 1084, 1827, 2716, 2890, 2914
HspAI GCGC 1 cut(s) 2908
KflI GGGWCCC 2 cut(s) 1753, 2103
KpnI GGTACC 2 cut(s) 1686, 2288
KroI GCCGGC 1 cut(s) 51
KroNI GCCGGC 1 cut(s) 53
Ksp22I TGATCA 2 cut(s) 1027, 1639
KspAI GTTAAC 2 cut(s) 2064, 2272
Kzo9I GATC 9 cut(s) 40, 532, 865, 944, 1027, 1639, 2161, 2238, 3036
LguI GCTCTTC 1 cut(s) 2703
LmnI GCTCC 8 cut(s) 1262, 1742, 2125, 2413, 2424, 2629, 2817, 2956
MabI ACCWGGT 1 cut(s) 829
MaeII ACGT 3 cut(s) 928, 1560, 2277
MaeIII GTNAC 6 cut(s) 178, 488, 766, 979, 1043, 1418
MalI GATC 9 cut(s) 42, 534, 867, 946, 1029, 1641, 2163, 2240, 3038
MboI GATC 9 cut(s) 40, 532, 865, 944, 1027, 1639, 2161, 2238, 3036
MfeI CAATTG 3 cut(s) 399, 717, 2586
MflI RGATCY 4 cut(s) 40, 532, 944, 3036
MhlI GDGCHC 7 cut(s) 268, 312, 1321, 1784, 1831, 2020, 2658
MmeI TCCRAC 4 cut(s) 126, 1087, 1214, 2070
MroNI GCCGGC 1 cut(s) 51
MroXI GAANNNNTTC 1 cut(s) 1185
MslI CAYNNNNRTG 5 cut(s) 1324, 1709, 1777, 2484, 2982
MspA1I CMGCKG 2 cut(s) 334, 1868
MspI CCGG 6 cut(s) 52, 165, 1444, 2351, 2568, 2649
MspR9I CCNGG 4 cut(s) 362, 831, 1445, 2014
MunI CAATTG 3 cut(s) 399, 717, 2586
Mva1269I GAATGC 4 cut(s) 700, 1627, 1927, 1973
MvaI CCWGG 3 cut(s) 362, 831, 2014
NaeI GCCGGC 1 cut(s) 53
NciI CCSGG 1 cut(s) 1445
NcoI CCATGG 3 cut(s) 649, 1080, 2712
NdeI CATATG 2 cut(s) 2481, 2922
NdeII GATC 9 cut(s) 40, 532, 865, 944, 1027, 1639, 2161, 2238, 3036
NgoMIV GCCGGC 1 cut(s) 51
NlaIII CATG 8 cut(s) 191, 591, 653, 1084, 1827, 2716, 2890, 2914
NmeAIII GCCGAG 3 cut(s) 163, 705, 980
NmuCI GTSAC 3 cut(s) 178, 488, 1043
NspI RCATGY 1 cut(s) 2890
OliI CACNNNNGTG 1 cut(s) 1777
PceI AGGCCT 2 cut(s) 129, 1127
PciSI GCTCTTC 1 cut(s) 2703
PctI GAATGC 4 cut(s) 700, 1627, 1927, 1973
PdiI GCCGGC 1 cut(s) 53
PdmI GAANNNNTTC 1 cut(s) 1185
PfeI GAWTC 9 cut(s) 259, 1036, 1075, 1172, 1181, 1289, 1534, 1676, 2574
PfoI TCCNGGA 1 cut(s) 1443
PpuMI RGGWCCY 4 cut(s) 214, 1753, 1861, 2103
PshAI GACNNNNGTC 1 cut(s) 495
PsiI TTATAA 2 cut(s) 1502, 2786
Psp5II RGGWCCY 4 cut(s) 214, 1753, 1861, 2103
Psp6I CCWGG 3 cut(s) 360, 829, 2012
PspFI CCCAGC 1 cut(s) 1864
PspGI CCWGG 3 cut(s) 360, 829, 2012
PspOMI GGGCCC 2 cut(s) 264, 2016
PspPPI RGGWCCY 4 cut(s) 214, 1753, 1861, 2103
PstI CTGCAG 2 cut(s) 1275, 1533
PstNI CAGNNNCTG 2 cut(s) 1481, 1553
PsuI RGATCY 4 cut(s) 40, 532, 944, 3036
PvuII CAGCTG 2 cut(s) 334, 1868
RsaI GTAC 8 cut(s) 68, 462, 1684, 1904, 2184, 2286, 2636, 2727
RsaNI GTAC 8 cut(s) 67, 461, 1683, 1903, 2183, 2285, 2635, 2726
RseI CAYNNNNRTG 5 cut(s) 1324, 1709, 1777, 2484, 2982
SalI GTCGAC 1 cut(s) 2540
SapI GCTCTTC 1 cut(s) 2703
Sau3AI GATC 9 cut(s) 40, 532, 865, 944, 1027, 1639, 2161, 2238, 3036
ScrFI CCNGG 4 cut(s) 362, 831, 1445, 2014
SduI GDGCHC 7 cut(s) 268, 312, 1321, 1784, 1831, 2020, 2658
SexAI ACCWGGT 1 cut(s) 829
SfcI CTRYAG 4 cut(s) 366, 1271, 1529, 2524
SinI GGWCC 6 cut(s) 214, 497, 1204, 1753, 1861, 2103
SmiMI CAYNNNNRTG 5 cut(s) 1324, 1709, 1777, 2484, 2982
SmlI CTYRAG 3 cut(s) 608, 1016, 1853
SmoI CTYRAG 3 cut(s) 608, 1016, 1853
SseBI AGGCCT 2 cut(s) 129, 1127
SsiI CCGC 2 cut(s) 88, 2954
SspI AATATT 1 cut(s) 1519
StuI AGGCCT 2 cut(s) 129, 1127
StyD4I CCNGG 4 cut(s) 360, 829, 1443, 2012
StyI CCWWGG 7 cut(s) 130, 649, 786, 1080, 1200, 1687, 2712
TaaI ACNGT 8 cut(s) 511, 1232, 1306, 1477, 1508, 1786, 2051, 2767
TaiI ACGT 3 cut(s) 931, 1563, 2280
TaqI TCGA 5 cut(s) 408, 594, 1170, 2361, 2541
TaqII GACCGA 1 cut(s) 674
TatI WGTACW 3 cut(s) 1902, 2182, 2634
TauI GCSGC 1 cut(s) 91
TfiI GAWTC 9 cut(s) 259, 1036, 1075, 1172, 1181, 1289, 1534, 1676, 2574
TseFI GTSAC 3 cut(s) 178, 488, 1043
Tsp45I GTSAC 3 cut(s) 178, 488, 1043
TspDTI ATGAA 9 cut(s) 207, 699, 958, 1778, 1782, 1931, 2408, 2453, 2551
TspGWI ACGGA 1 cut(s) 687
VneI GTGCAC 2 cut(s) 1317, 1780
VpaK11BI GGWCC 6 cut(s) 214, 497, 1204, 1753, 1861, 2103
XagI CCTNNNNNAGG 1 cut(s) 1976
XapI RAATTY 8 cut(s) 19, 404, 797, 1248, 1342, 1585, 2291, 2558
XbaI TCTAGA 1 cut(s) 1609
XceI RCATGY 1 cut(s) 2890
XmaJI CCTAGG 1 cut(s) 1687
XmiI GTMKAC 3 cut(s) 1429, 2246, 2541
XmnI GAANNNNTTC 1 cut(s) 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.