Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0002217

Physical Location & Seq

Genomic Coordinates Reverse (-)

33496982 .. 33503071

Gene Structure 6090 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g39370.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3255 bp

ATGCTTCCTAGAAAGAGAGCTTGTGAAGGAGAAGAAGAAGAAGGAGACGGTGACGTTGACAAGGCCACTGGTACTACTAGCTCGGCTTCTTCGGCGTCTTTGATTAAGAAGCTGCGGATCGGATCCGAATCGGCGGTTAATAACAGCAGCAGCAGTAACGGTAGCGGCGGAAGCGTGGTTGGGAACGACGTGCCGATCATGGCTTTGGGAGATTCCAACGCCGGGGATATCGATGAGGATCTGCACAGCCGGCAGCTTGCCGTCTATGGCCGGGAGACTATGAGGAGGCTCTTCGCATCCAATGTTCTGATTTCAGGGATACAGGGCCTCGGTGCCGAGATTGCCAAGAATCTCATTCTTGCTGGTGTGAAGACTGTGACCTTGCATGATGAAGGGAAAGTAGAGCTTTGGGATTTGTCCAGCAATTTTCTTTTCACAGAGGATGATGTTGGTAAGAACAGGGCACTTGCTTCTGTTCAGAAGCTGCAGGAACTCAACAATGCTGTGGTTGTTCATACCTTGACAACTCCATTGACTAAGGAGCAGCTTTCTGATTTCCAGGCTGTTGTGTTCACTGATATCAGTTACGAGAAAGCTATTGAACTCAATGATTATTGCCATAATCATCAGCCCCCAATCGCCTTCATAAGAACCGAAGTTAGAGGCCTTTTTGGTTCTGTATTTTGCGACTTTGGACCTGAATTCACTGTCTTTGATGTTGATGGGGAGGAGCCACACACGGGTATTATTGCATCAATCAGCAACGACAACCCTGCTCTTGTGTCATGTGTCGATGATGAAAGACTTGAGTTTCAGGATGGGGATCTTGTTGTGTTCTCAGAAGTTCATGGAATGACAGAACTAAATGATGGAAAGCCAAGGAAGATAAAAAATGCTAGGGCTTACTCCTTTACGCTTGAGGAGGACACCTCAGGTTTTGGTACCTATGAGAAAGGTGGCATTGTCACACAAGCGAAGCAGCCAAAAGTGTTGAATTTCAAGCCATTGAGGGAAGCACTCAATAATCCTGGCGATTTTCTTCTTAGTGATTTCTCCAAGTTTGATCGCCCTCCTCTCCTGCACTTGGCATTCCAAGCACTGGACAAATTTGTTTCTGAGTTGGGACGATTCCCTGTTGCTGGTTCAGAAGAGGATGCTCAAAAGCTTATATCTGTTGCTAGTAATATTAATGATAAATTGGGGGATGGCAGGCTGGAAGATCTTAATCCAAAGCTTTTGCGCCACTTTGCCTTTGGTGCAAAGGCTGTACTAAATCCCATGGCTGCCATGTTTGGTGGTATTGTTGGTCAAGAGGTTGTAAAAGCTTGCTCTGGAAAGTTTCATCCGCTCTTCCAGTTCTTCTACTTTGACTCGGTAGAATCACTTCCAACGGAGCCCCTGGATTCCAGTGATTTGAAACCATTGAATAGCCGTTATGATGCACAAATTTCAGTTTTTGGGTCCAAGCTACAGAAGAAACTGGAGGATGCCAATGTATTCATGGTTGGATCTGGTGCACTCGGATGTGAGTTGTTGAAAAATGTTGCCCTGATGGGAGTTTCATGTGGCAACCAGGGGAAGCTAACAATCACTGACGATGATGTGATTGAGAAGAGTAACCTCAGTAGGCAGTTCCTCTTCCGTGATTGGAATATTGGACAGGCAAAGTCTACAGTTGCTGCTTCTGCCGCTGCATCAATAAATCCCCGTCTCAACACTGTGGCGTTGCAGAACAGAGTTGGCCCTGAAACTGAGAATGTGTTTGATGACACCTTCTGGGAAAATTTAAGTGTTGTTATTAATGCTCTAGATAATGTCAATGCCAGGCTCTATGTTGATCAGAGGTGCTTATATTTCCAGAAGGCACTTCTTGAGTCGGGTACTCTTGGTGCAAAATGCAACACTCAGATGGTTATTCCTCACCTAACAGAAAACTATGGTGCCTCTAGAGATCCTCCAGAGAAACAAGCACCCATGTGTACTGTGCACTCATTTCCACACAACATTGACCACTGTTTAACGTGGGCTCGATCCGAGTTTGAGGGTTTGCTTGAGAAAACTCCAGCTGAAGTGAATGCATATTTATCCAAGCCAAGTGAATATACCGCTGCAATGAGTAATGCTGGGGATGCACAGGCCAGGGACACCTTGGAGCGAGTTCTTGAGTGCCTTGCCAGAGAAAGATGCGAAACATTCCAAGATTGTATTGCCTGGGCGCGCCTTAAGTTTGAAGATTATTTTTCCGACCGGGTAAAACAGCTGACATATACTTTTCCTGAAGATGCTGCAACTAGTACCGGGGCTCCATTCTGGTCTGCCCCCAAGCGATTCCCCCGTGCACTGCAGTTCTCAGCTACTGATCCTGGCCATCTTCATTTTGTTATGGCAGCATCCATACTCAGGGCAGAGACATTTGGGATCCCAATCCCCGACTGGGTTAGGAATTCTAAGAAGTTGTCTGAAGCTGTTGAGAAAGTGGAAGTCCCAGACTTTCAGCCCAAGAAAGATGCTAAAATAGTGACAGATGACAAGGCCACCAATTTAACTCCTCAATCCATAGATGATGCACAGGTTATTAATGAATTGATTATCAAGTTAGAGCAATGTCGAGAGAAACTCCCACCAGGCTTCAGGATGAAACCGATTCAGTTCGAGAAGGATGATGATACCAATTACCATATGGATATGATAGCTGGGCTTGCTAATATGAGGGCTAGGAATTACAGCATTCCTGAGGTGGACAAGCTGAAAGCCAAGTTTATTGCTGGTAGGATTATCCCTGCAATTGCAACTTCCACCGCAATGGCCACAGGACTTGTGTGCCTGGAACTTTACAAGGTTCTGGATGGTGGCCACAAACTGGAAGACTACAGAAATACATTTGCAAACCTAGCGCTACCTTTGTTCTCCATGGCTGAGCCGGTTCCACCCAAGGTCATCAAGCACCAGGACATGAAATGGACTGTGTGGGATAGGTGGATCCTGAGGGGCAATCCTACTTTGAGGGAACTTCTTCAGTGGCTTAAGGATAAGGGGCTGAATGCTTATAGCATCTCGTGTGGAAGCTCTCTGCTTTTTAACAGCATGTTTGCTCGGCACAAAGACCGAATGGACAAGAAGGTGGTGGATCTGGCCAAGGATGTGGCGAAGGTGGAGATTCCTCCATATCGTTGCCACTTGGATGTCGTTGTTGCTTGCGAGGATGATGAGGACAACGACATTGACATCCCTCTGGTATCAATTTACTTCCGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030054 GO:0032446 GO:0033554 GO:0036211 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 ko05012 map04120 map05012

Pfam Domains

Protein Families

E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1085

Amino Acids

120.09

Weight (kDa)

5.18

Isoelectric Point (pI)

32.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	82 - 457	1.2e-28	ThiF family
E1_FCCH	PF16190	256 - 326	9.5e-28	Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB	PF16191	327 - 397	4.2e-26	Ubiquitin-activating enzyme E1 four-helix bundle
ThiF	PF00899	479 - 971	3.8e-58	ThiF family
UBA_E1_SCCH	PF10585	666 - 919	2.2e-87	Ubiquitin-activating enzyme, SCCH domain
E1_UFD	PF09358	990 - 1079	2.2e-23	Ubiquitin fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30110 AT5G06460
fragaria_vesca	FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_6g10930 FvH4_6g10930 FvH4_6g10930
malus_domestica	MD04G1163500.v1.1 MD11G1065700.v1.1 MD12G1177300.v1.1
prunus_persica	Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G282900_v2.0.a1
pyrus_communis	pycom04g14620 pycom11g05570 pycom12g16790
rosa_chinensis	RchiOBHm_Chr3g0462201 RchiOBHm_Chr5g0070911
rosa_laevigata	RLG00000024878 RLG00000036170
rosa_multiflora	Rmu_sc0002944.1_g000004 Rmu_sc0005970.1_g000009 Rmu_sc0006715.1_g000003
rosa_roxburghii	Rroxscaffold_1G00010290 Rroxscaffold_6G00417690
rosa_rugosa	Rorug03G0054900 Rorug05G0408500 Rorug05G0408500 Rorug05G0408500
rosa_samantha	Rh3CG119500 Rh5AG464600 Rh5DG494900
rosa_wichuraiana	Rw3G009590 Rw5G043220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	941
AccB1I	GGYRCC	3 cut(s)	332, 941, 1940
AccB7I	CCANNNNNTGG	2 cut(s)	1099, 2860
AccBSI	CCGCTC	1 cut(s)	1348
AccI	GTMKAC	1 cut(s)	1670
AccII	CGCG	1 cut(s)	2218
AciI	CCGC	8 cut(s)	115, 134, 165, 168, 1346, 1689, 2106, 2799
AcoI	YGGCCR	5 cut(s)	268, 2365, 2805, 2851, 3132
AcsI	RAATTY	6 cut(s)	701, 994, 1106, 1446, 1783, 2443
AcuI	CTGAAG	5 cut(s)	2088, 2298, 2481, 2614, 2999
AcyI	GRCGYC	1 cut(s)	95
AfaI	GTAC	6 cut(s)	73, 943, 1269, 1882, 1981, 2296
AfeI	AGCGCT	1 cut(s)	2895
AfiI	CCNNNNNNNGG	8 cut(s)	222, 740, 1084, 1099, 1139, 2400, 2434, 2860
AflII	CTTAAG	2 cut(s)	2222, 3023
AgsI	TTSAA	7 cut(s)	602, 994, 1000, 1417, 1426, 1537, 2231
AhlI	ACTAGT	1 cut(s)	2291
AjiI	CACGTC	1 cut(s)	190
AleI	CACNNNNGTG	1 cut(s)	1975
Alw21I	GWGCWC	3 cut(s)	1519, 1989, 2341
Alw26I	GTCTC	4 cut(s)	39, 269, 1715, 2402
Alw44I	GTGCAC	3 cut(s)	1515, 1985, 2337
AlwNI	CAGNNNCTG	3 cut(s)	484, 1679, 2357
Aor51HI	AGCGCT	1 cut(s)	2895
ApaLI	GTGCAC	3 cut(s)	1515, 1985, 2337
ApoI	RAATTY	6 cut(s)	701, 994, 1106, 1446, 1783, 2443
AscI	GGCGCGCC	1 cut(s)	2216
AseI	ATTAAT	3 cut(s)	1188, 1800, 2577
Asp700I	GAANNNNTTC	2 cut(s)	1383, 3012
Asp718I	GGTACC	1 cut(s)	941
AspLEI	GCGC	4 cut(s)	1242, 2218, 2220, 2896
AspS9I	GGNCC	4 cut(s)	325, 695, 1461, 1742
AsuC2I	CCSGG	4 cut(s)	223, 272, 2249, 2299
AsuHPI	GGTGA	2 cut(s)	62, 1913
AvaII	GGWCC	2 cut(s)	695, 1461
AxyI	CCTNAGG	3 cut(s)	931, 2733, 2984
BaeGI	GKGCMC	4 cut(s)	466, 1519, 1989, 2341
BalI	TGGCCA	4 cut(s)	2367, 2807, 2853, 3134
BamHI	GGATCC	3 cut(s)	122, 2418, 2979
BanI	GGYRCC	3 cut(s)	332, 941, 1940
BanII	GRGCYC	3 cut(s)	1398, 2029, 2305
BarI	GAAGNNNNNNTAC	2 cut(s)	1368, 1400
BauI	CACGAG	1 cut(s)	3055
BbsI	GAAGAC	2 cut(s)	377, 2871
Bbv12I	GWGCWC	3 cut(s)	1519, 1989, 2341
BccI	CCATC	8 cut(s)	716, 812, 863, 1199, 1546, 1903, 2376, 2840
BceAI	ACGGC	2 cut(s)	245, 1416
BcgI	CGANNNNNNTGC	2 cut(s)	755, 789
BciVI	GTATCC	1 cut(s)	312
BclI	TGATCA	1 cut(s)	1837
BcnI	CCSGG	4 cut(s)	223, 272, 2249, 2299
BcoDI	GTCTC	4 cut(s)	39, 269, 1715, 2402
BcuI	ACTAGT	1 cut(s)	2291
BfaI	CTAG	9 cut(s)	9, 78, 897, 1179, 1808, 1947, 2292, 2715, 2891
BfmI	CTRYAG	5 cut(s)	485, 1469, 1671, 2342, 2869
BfoI	RGCGCY	1 cut(s)	2897
BfrI	CTTAAG	2 cut(s)	2222, 3023
BfuI	GTATCC	1 cut(s)	312
BglII	AGATCT	1 cut(s)	1219
BlpI	GCTNAGC	1 cut(s)	2916
Bme18I	GGWCC	2 cut(s)	695, 1461
BmgBI	CACGTC	1 cut(s)	190
BmgT120I	GGNCC	4 cut(s)	325, 695, 1461, 1742
BmrI	ACTGGG	1 cut(s)	2443
BmuI	ACTGGG	1 cut(s)	2443
BpiI	GAAGAC	2 cut(s)	377, 2871
BplI	GAGNNNNNCTC	4 cut(s)	914, 946, 2601, 2633
BpmI	CTGGAG	3 cut(s)	1502, 1941, 2046
Bpu1102I	GCTNAGC	1 cut(s)	2916
BpuEI	CTTGAG	5 cut(s)	827, 938, 1892, 2072, 2183
BpuMI	CCSGG	4 cut(s)	223, 272, 2249, 2299
Bsa29I	ATCGAT	1 cut(s)	231
BsaBI	GATNNNNATC	5 cut(s)	127, 237, 822, 1224, 2423
BsaHI	GRCGYC	1 cut(s)	95
BsaXI	ACNNNNNCTCC	2 cut(s)	2287, 2317
Bsc4I	CCNNNNNNNGG	8 cut(s)	222, 740, 1084, 1099, 1139, 2400, 2434, 2860
Bse118I	RCCGGY	2 cut(s)	249, 2920
Bse1I	ACTGG	7 cut(s)	73, 1104, 1354, 1407, 1485, 2438, 2865
Bse21I	CCTNAGG	3 cut(s)	931, 2733, 2984
Bse3DI	GCAATG	3 cut(s)	2118, 2609, 2808
Bse8I	GATNNNNATC	5 cut(s)	127, 237, 822, 1224, 2423
BseCI	ATCGAT	1 cut(s)	231
BseJI	GATNNNNATC	5 cut(s)	127, 237, 822, 1224, 2423
BseLI	CCNNNNNNNGG	8 cut(s)	222, 740, 1084, 1099, 1139, 2400, 2434, 2860
BseMI	GCAATG	3 cut(s)	2118, 2609, 2808
BseNI	ACTGG	7 cut(s)	73, 1104, 1354, 1407, 1485, 2438, 2865
BsePI	GCGCGC	1 cut(s)	2216
BseRI	GAGGAG	5 cut(s)	298, 743, 935, 1062, 2538
BseSI	GKGCMC	4 cut(s)	466, 1519, 1989, 2341
BseYI	CCCAGC	2 cut(s)	2123, 2693
BsgI	GTGCAG	2 cut(s)	227, 1064
Bsh1236I	CGCG	1 cut(s)	2218
Bsh1285I	CGRYCG	1 cut(s)	2248
BshNI	GGYRCC	3 cut(s)	332, 941, 1940
BshVI	ATCGAT	1 cut(s)	231
BsiEI	CGRYCG	1 cut(s)	2248
BsiHKAI	GWGCWC	3 cut(s)	1519, 1989, 2341
BsiSI	CCGG	6 cut(s)	222, 250, 271, 2248, 2298, 2921
BslFI	GGGAC	3 cut(s)	1137, 2156, 2468
BslI	CCNNNNNNNGG	8 cut(s)	222, 740, 1084, 1099, 1139, 2400, 2434, 2860
BsmAI	GTCTC	4 cut(s)	39, 269, 1715, 2402
BsmBI	CGTCTC	2 cut(s)	39, 1715
BsmFI	GGGAC	3 cut(s)	1137, 2156, 2468
BsmI	GAATGC	4 cut(s)	1088, 2080, 2727, 3046
Bsp1286I	GDGCHC	7 cut(s)	466, 1398, 1519, 1989, 2029, 2305, 2341
Bsp1720I	GCTNAGC	1 cut(s)	2916
Bsp19I	CCATGG	2 cut(s)	1278, 2910
BspACI	CCGC	8 cut(s)	115, 134, 165, 168, 1346, 1689, 2106, 2799
BspDI	ATCGAT	1 cut(s)	231
BspFNI	CGCG	1 cut(s)	2218
BspMAI	CTGCAG	2 cut(s)	489, 2346
BspQI	GCTCTTC	2 cut(s)	296, 1355
BspT107I	GGYRCC	3 cut(s)	332, 941, 1940
BspTI	CTTAAG	2 cut(s)	2222, 3023
BsrBI	CCGCTC	1 cut(s)	1348
BsrDI	GCAATG	3 cut(s)	2118, 2609, 2808
BsrFI	RCCGGY	2 cut(s)	249, 2920
BsrI	ACTGG	7 cut(s)	73, 1104, 1354, 1407, 1485, 2438, 2865
BssAI	RCCGGY	2 cut(s)	249, 2920
BssHII	GCGCGC	1 cut(s)	2216
BssNI	GRCGYC	1 cut(s)	95
BssSI	CACGAG	1 cut(s)	3055
BssT1I	CCWWGG	6 cut(s)	878, 1278, 2148, 2910, 2931, 3135
Bst2BI	CACGAG	1 cut(s)	3055
Bst4CI	ACNGT	9 cut(s)	50, 161, 376, 709, 1675, 1720, 1984, 2015, 2965
Bst6I	CTCTTC	5 cut(s)	296, 1143, 1355, 1607, 1643
BstACI	GRCGYC	1 cut(s)	95
BstAFI	CTTAAG	2 cut(s)	2222, 3023
BstC8I	GCNNGC	7 cut(s)	251, 258, 1211, 1327, 2218, 2700, 3196
BstDSI	CCRYGG	2 cut(s)	1278, 2910
BstFNI	CGCG	1 cut(s)	2218
BstH2I	RGCGCY	1 cut(s)	2897
BstHHI	GCGC	4 cut(s)	1242, 2218, 2220, 2896
BstMAI	GTCTC	4 cut(s)	39, 269, 1715, 2402
BstMCI	CGRYCG	1 cut(s)	2248
BstNSI	RCATGY	1 cut(s)	3088
BstSFI	CTRYAG	5 cut(s)	485, 1469, 1671, 2342, 2869
BstSLI	GKGCMC	4 cut(s)	466, 1519, 1989, 2341
BstUI	CGCG	1 cut(s)	2218
BstV2I	GAAGAC	2 cut(s)	377, 2871
BstX2I	RGATCY	9 cut(s)	122, 238, 823, 1219, 1508, 1951, 2418, 2979, 3127
BstXI	CCANNNNNNTGG	2 cut(s)	2803, 3142
BstYI	RGATCY	9 cut(s)	122, 238, 823, 1219, 1508, 1951, 2418, 2979, 3127
Bsu15I	ATCGAT	1 cut(s)	231
Bsu36I	CCTNAGG	3 cut(s)	931, 2733, 2984
BsuI	GTATCC	1 cut(s)	312
BsuTUI	ATCGAT	1 cut(s)	231
BtgI	CCRYGG	2 cut(s)	1278, 2910
BtrI	CACGTC	1 cut(s)	190
BtsI	GCAGTG	1 cut(s)	2339
Cac8I	GCNNGC	7 cut(s)	251, 258, 1211, 1327, 2218, 2700, 3196
CaiI	CAGNNNCTG	3 cut(s)	484, 1679, 2357
CfoI	GCGC	4 cut(s)	1242, 2218, 2220, 2896
Cfr10I	RCCGGY	2 cut(s)	249, 2920
Cfr13I	GGNCC	4 cut(s)	325, 695, 1461, 1742
ClaI	ATCGAT	1 cut(s)	231
CseI	GACGC	1 cut(s)	84
Csp6I	GTAC	6 cut(s)	72, 942, 1268, 1881, 1980, 2295
CspCI	CAANNNNNGTGG	2 cut(s)	2797, 2832
CviQI	GTAC	6 cut(s)	72, 942, 1268, 1881, 1980, 2295
EaeI	YGGCCR	5 cut(s)	268, 2365, 2805, 2851, 3132
Eam1104I	CTCTTC	5 cut(s)	296, 1143, 1355, 1607, 1643
EarI	CTCTTC	5 cut(s)	296, 1143, 1355, 1607, 1643
EciI	GGCGGA	1 cut(s)	183
Eco130I	CCWWGG	6 cut(s)	878, 1278, 2148, 2910, 2931, 3135
Eco147I	AGGCCT	1 cut(s)	666
Eco24I	GRGCYC	3 cut(s)	1398, 2029, 2305
Eco32I	GATATC	2 cut(s)	229, 580
Eco47I	GGWCC	2 cut(s)	695, 1461
Eco47III	AGCGCT	1 cut(s)	2895
Eco57I	CTGAAG	5 cut(s)	2088, 2298, 2481, 2614, 2999
Eco81I	CCTNAGG	3 cut(s)	931, 2733, 2984
EcoO109I	RGGNCCY	1 cut(s)	325
EcoRI	GAATTC	2 cut(s)	701, 2443
EcoRV	GATATC	2 cut(s)	229, 580
EcoT14I	CCWWGG	6 cut(s)	878, 1278, 2148, 2910, 2931, 3135
EcoT22I	ATGCAT	1 cut(s)	2080
EcoT38I	GRGCYC	3 cut(s)	1398, 2029, 2305
ErhI	CCWWGG	6 cut(s)	878, 1278, 2148, 2910, 2931, 3135
Esp3I	CGTCTC	2 cut(s)	39, 1715
FalI	AAGNNNNNCTT	2 cut(s)	390, 422
FaqI	GGGAC	3 cut(s)	1137, 2156, 2468
FauNDI	CATATG	1 cut(s)	2679
FbaI	TGATCA	1 cut(s)	1837
FblI	GTMKAC	1 cut(s)	1670
FriOI	GRGCYC	3 cut(s)	1398, 2029, 2305
FspBI	CTAG	9 cut(s)	9, 78, 897, 1179, 1808, 1947, 2292, 2715, 2891
GlaI	GCGC	4 cut(s)	1241, 2217, 2219, 2895
GsaI	CCCAGC	2 cut(s)	2127, 2697
GsuI	CTGGAG	3 cut(s)	1502, 1941, 2046
HaeII	RGCGCY	1 cut(s)	2897
HapII	CCGG	6 cut(s)	222, 250, 271, 2248, 2298, 2921
HgaI	GACGC	1 cut(s)	84
HhaI	GCGC	4 cut(s)	1242, 2218, 2220, 2896
Hin1I	GRCGYC	1 cut(s)	95
Hin6I	GCGC	4 cut(s)	1240, 2216, 2218, 2894
HinP1I	GCGC	4 cut(s)	1240, 2216, 2218, 2894
HincII	GTYRAC	1 cut(s)	58
HindII	GTYRAC	1 cut(s)	58
HindIII	AAGCTT	3 cut(s)	1163, 1232, 1323
HpaII	CCGG	6 cut(s)	222, 250, 271, 2248, 2298, 2921
HphI	GGTGA	2 cut(s)	62, 1913
Hpy166II	GTNNAC	8 cut(s)	58, 573, 1517, 1671, 1980, 1987, 2339, 2740
Hpy8I	GTNNAC	8 cut(s)	58, 573, 1517, 1671, 1980, 1987, 2339, 2740
Hpy99I	CGWCG	1 cut(s)	191
HpyAV	CCTTC	9 cut(s)	20, 35, 386, 652, 1783, 1855, 2650, 3112, 3142
HpyCH4III	ACNGT	9 cut(s)	50, 161, 376, 709, 1675, 1720, 1984, 2015, 2965
HpyCH4IV	ACGT	3 cut(s)	54, 189, 2021
HpySE526I	ACGT	3 cut(s)	54, 189, 2021
Hsp92I	GRCGYC	1 cut(s)	95
HspAI	GCGC	4 cut(s)	1240, 2216, 2218, 2894
KpnI	GGTACC	1 cut(s)	945
KroI	GCCGGC	1 cut(s)	249
KroNI	GCCGGC	1 cut(s)	251
Ksp22I	TGATCA	1 cut(s)	1837
LguI	GCTCTTC	2 cut(s)	296, 1355
LmnI	GCTCC	5 cut(s)	541, 730, 1393, 2152, 2308
MaeI	CTAG	9 cut(s)	9, 78, 897, 1179, 1808, 1947, 2292, 2715, 2891
MaeII	ACGT	3 cut(s)	54, 189, 2021
MaeIII	GTNAC	7 cut(s)	50, 155, 376, 584, 964, 1616, 2518
MbiI	CCGCTC	1 cut(s)	1348
MfeI	CAATTG	1 cut(s)	2784
MflI	RGATCY	9 cut(s)	122, 238, 823, 1219, 1508, 1951, 2418, 2979, 3127
MhlI	GDGCHC	7 cut(s)	466, 1398, 1519, 1989, 2029, 2305, 2341
MlsI	TGGCCA	4 cut(s)	2367, 2807, 2853, 3134
MluNI	TGGCCA	4 cut(s)	2367, 2807, 2853, 3134
MlyI	GAGTC	2 cut(s)	1364, 1883
MmeI	TCCRAC	4 cut(s)	240, 1412, 1486, 2268
Mox20I	TGGCCA	4 cut(s)	2367, 2807, 2853, 3134
Mph1103I	ATGCAT	1 cut(s)	2080
MroNI	GCCGGC	1 cut(s)	249
MroXI	GAANNNNTTC	2 cut(s)	1383, 3012
MscI	TGGCCA	4 cut(s)	2367, 2807, 2853, 3134
MslI	CAYNNNNRTG	5 cut(s)	1522, 1907, 1975, 2801, 3180
Msp20I	TGGCCA	4 cut(s)	2367, 2807, 2853, 3134
MspA1I	CMGCKG	4 cut(s)	1691, 2066, 2108, 2260
MspCI	CTTAAG	2 cut(s)	2222, 3023
MspI	CCGG	6 cut(s)	222, 250, 271, 2248, 2298, 2921
MunI	CAATTG	1 cut(s)	2784
Mva1269I	GAATGC	4 cut(s)	1088, 2080, 2727, 3046
MvnI	CGCG	1 cut(s)	2218
NaeI	GCCGGC	1 cut(s)	251
NciI	CCSGG	4 cut(s)	223, 272, 2249, 2299
NcoI	CCATGG	2 cut(s)	1278, 2910
NdeI	CATATG	1 cut(s)	2679
NgoMIV	GCCGGC	1 cut(s)	249
NmeAIII	GCCGAG	3 cut(s)	62, 361, 3073
NmuCI	GTSAC	4 cut(s)	50, 376, 964, 2518
NsiI	ATGCAT	1 cut(s)	2080
NspI	RCATGY	1 cut(s)	3088
OliI	CACNNNNGTG	1 cut(s)	1975
PalAI	GGCGCGCC	1 cut(s)	2216
PauI	GCGCGC	1 cut(s)	2216
PceI	AGGCCT	1 cut(s)	666
PciSI	GCTCTTC	2 cut(s)	296, 1355
PctI	GAATGC	4 cut(s)	1088, 2080, 2727, 3046
PdiI	GCCGGC	1 cut(s)	251
PdmI	GAANNNNTTC	2 cut(s)	1383, 3012
PfeI	GAWTC	9 cut(s)	128, 212, 349, 1128, 1379, 1403, 2328, 2644, 3157
PflMI	CCANNNNNTGG	2 cut(s)	1099, 2860
PleI	GAGTC	2 cut(s)	1364, 1882
PpsI	GAGTC	2 cut(s)	1364, 1882
PshBI	ATTAAT	3 cut(s)	1188, 1800, 2577
PspFI	CCCAGC	2 cut(s)	2123, 2693
PspPI	GGNCC	4 cut(s)	325, 695, 1461, 1742
PstI	CTGCAG	2 cut(s)	489, 2346
PstNI	CAGNNNCTG	3 cut(s)	484, 1679, 2357
PsuI	RGATCY	9 cut(s)	122, 238, 823, 1219, 1508, 1951, 2418, 2979, 3127
PteI	GCGCGC	1 cut(s)	2216
PvuII	CAGCTG	2 cut(s)	2066, 2260
RsaI	GTAC	6 cut(s)	73, 943, 1269, 1882, 1981, 2296
RsaNI	GTAC	6 cut(s)	72, 942, 1268, 1881, 1980, 2295
RseI	CAYNNNNRTG	5 cut(s)	1522, 1907, 1975, 2801, 3180
SapI	GCTCTTC	2 cut(s)	296, 1355
Sau96I	GGNCC	4 cut(s)	325, 695, 1461, 1742
SchI	GAGTC	2 cut(s)	1364, 1883
SduI	GDGCHC	7 cut(s)	466, 1398, 1519, 1989, 2029, 2305, 2341
SfcI	CTRYAG	5 cut(s)	485, 1469, 1671, 2342, 2869
SgsI	GGCGCGCC	1 cut(s)	2216
SinI	GGWCC	2 cut(s)	695, 1461
SmiMI	CAYNNNNRTG	5 cut(s)	1522, 1907, 1975, 2801, 3180
SmlI	CTYRAG	7 cut(s)	806, 917, 1871, 2051, 2162, 2222, 3023
SmoI	CTYRAG	7 cut(s)	806, 917, 1871, 2051, 2162, 2222, 3023
SpeI	ACTAGT	1 cut(s)	2291
SseBI	AGGCCT	1 cut(s)	666
SsiI	CCGC	8 cut(s)	115, 134, 165, 168, 1346, 1689, 2106, 2799
SspI	AATATT	2 cut(s)	1186, 1654
SspMI	CTAG	9 cut(s)	9, 78, 897, 1179, 1808, 1947, 2292, 2715, 2891
StuI	AGGCCT	1 cut(s)	666
StyI	CCWWGG	6 cut(s)	878, 1278, 2148, 2910, 2931, 3135
TaaI	ACNGT	9 cut(s)	50, 161, 376, 709, 1675, 1720, 1984, 2015, 2965
TaiI	ACGT	3 cut(s)	57, 192, 2024
TaqI	TCGA	5 cut(s)	231, 792, 2029, 2608, 2652
TaqII	GACCGA	1 cut(s)	3120
TatI	WGTACW	2 cut(s)	1267, 1979
TauI	GCSGC	2 cut(s)	168, 1691
TfiI	GAWTC	9 cut(s)	128, 212, 349, 1128, 1379, 1403, 2328, 2644, 3157
TseFI	GTSAC	4 cut(s)	50, 376, 964, 2518
Tsp45I	GTSAC	4 cut(s)	50, 376, 964, 2518
TspGWI	ACGGA	3 cut(s)	1406, 1631, 3239
Van91I	CCANNNNNTGG	2 cut(s)	1099, 2860
Vha464I	CTTAAG	2 cut(s)	2222, 3023
VneI	GTGCAC	3 cut(s)	1515, 1985, 2337
VpaK11BI	GGWCC	2 cut(s)	695, 1461
VspI	ATTAAT	3 cut(s)	1188, 1800, 2577
XapI	RAATTY	6 cut(s)	701, 994, 1106, 1446, 1783, 2443
XbaI	TCTAGA	2 cut(s)	1807, 1946
XceI	RCATGY	1 cut(s)	3088
XcmI	CCANNNNNNNNNTGG	5 cut(s)	1250, 1498, 2146, 2430, 2677
XmiI	GTMKAC	1 cut(s)	1670
XmnI	GAANNNNTTC	2 cut(s)	1383, 3012
XspI	CTAG	9 cut(s)	9, 78, 897, 1179, 1808, 1947, 2292, 2715, 2891
Zsp2I	ATGCAT	1 cut(s)	2080

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.