Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0002217

Physical Location & Seq

Genomic Coordinates Reverse (-)

77606479 .. 77613682

Gene Structure 7204 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG494900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2868 bp

ATGGTCAAATTAGGTTTAAAGTTTTGGGGGTGGGTTGCTGTAGCTTCGGCGCCGATCTTTGTCGGATTGGCGTTCCGAAGCTACTCCGGCGTCGGCGACGGTGCCGATTCGGTTGATTTTCCGGTTTTGTGTTTGGTTGTTTCGGTGATCGTCGGGTTTTGTGGCGTGTTCAGCAGCTTACTGCACTATATGCTTCCTAGAAAGAGACCTTGTGAAGGAGAAGAAGGAGACGGTGACGTTAACAAGACCACTGGTACCAGCTCGGCGGCTTCTGCGTCTTTGATCAAGAAGCTTCGGATCGAATCCTACGTTGCCGAATCGGCCATCAACAACAGCAGTAACAACAGCAGCAGCAACGGTAGCGGGAGCGTGGTTGAGAACGACGTGCCGATCATGGCTTTGGGAGATTCCAACGCCGGAGATATCGATGAGGATCTGCACAGCCGGCAGCTTGCCGTCTATGGCCGGGAGACGATGAGGAGGCTCTTTGCATCCAATGTTCTGATCTCAGGGGTACAGGGCCTCGGTGCTGAGATTGCCAAAAATCTCATTCTTGCTGGTGTGAAGTCTGTGACCTTGCATGATGAAGGGACTGTGGAGCTTTGGGATTTGTCCAGCAATTTTATTTTTACAGAGGATGATGTTGGTAAGAACAGGGCACTTGCTTCTGTTCAAAAGTTGCAGGAACTCAACAATGCTGTGATTGTTCATACCTTGACAACTCCATTGACTGCGGAGCAGCTTTCTGATTTCCAGGCTGTTGTATTCACTGATATCAGTTACGAGAAAGCTATTGAATTCAATGACTATTGTCATAATCATCAGCCGCCAATCGCCTTCATAAGAACCGAAGTTAGAGGCCTTTTTGGTTCTGTATTTTGTGACTTTGGACCTGAATTCACTGTTTTTGATGTTGATGGGGAGGAACCACACACGGGTATTATTGCATCAATCAGCAATGACAACCCTGCTATTGTGTCATGTGTCGATGATGAAAGACTTGAATTTCAGGATGGGGATCTTGTTGTGTTCTCAGAAGTTCATGGAATGACAGAACTAAATGATGGAAAGCCAAGGAAGATAAAAAATGCTAGGGCTTACTCATTTACTCTTGAGGAGGATACCTCAGGTTTTGGTACCTATGTGAAAGGTGGCATTGTCACACAAGTGAAGCAGCCAAAAGTGTTGAACTTCAAGCCATTGGGGGAAGCACTCAAGAATCCCGGTGATTTTCTTCTTAGTGATTTCTCCAAGTTTGATCACCCTCCTCTCCTACACTTGGCATTTCAAGCACTGGACAAATTTGTTTCTGAGTTGGGACGATTCCCTGTTGCTGGTTCAGAAGAGGATGCTCAGAAGCTTATATCTGTTGCTACTAACATTAATGATAAACTGGGAGATGGCAGGCTGGAAGATCTTAATCCAAAACTTTTGCGCCACTTCGCCTTTGGTGCAAAGGCAGTACTAAGTCCCATGGCTGCCATGTTTGGTGGTATTGTTGGTCAAGAGGTTGTAAAAGCTTGCTCTGGAAAGTTTCATCCACTCTTCCAGTTCTTCTACTTTGACTCAGTAGAATCACTTCCAACGGAGCCACTGGATTCCAGTGATTTGAAACCATTAAATAGCCGTTATGATGCACAGATTTCAGTTTTTGGGTCCAAGCTACAGAAGAAACTGGAGCATGCCAAAGTATTCATGGTTGGATCTGGTGCACTTGGATGTGAGTTGTTGAAAAATGTTGCCCTGATGGGAGTTTCATGTGGCGACCAAGGGAAGCTAACAATCACTGATGATGATGTAATTGAGAAGAGTAACCTCAGTAGGCAGTTCCTTTTCCGTGATTGGAACATTGGGCAGGCAAAGTCTACAGTTGCCGCTTCTGCTGCTGCATCAATAAATCCCCGTCTCAACATTGTAGCGTTGCAGAATAGAGTTGGCCCTGAAACTGAGAATGTGTTTGATGATACCTACTGGGAGAATTTAAGTGTTGTTATTAATGCACTAGATAATGTCAATGCCAGGCTCTATGTTGATCAGCGGTGCTTATATTTCCAGAAGGCACTTCTTGAGTCGGGTACTCTGGGTGCGAAATGCAACACTCAGATGGTTATTCCTCACCTAACAGAAAACTATGGTGCCTCTAGAGATCCTCCAGAGAAACAAGCACCCATGTGTACTGTCCACTCATTTCCACACAACATTGATCACTGTCTGACATGGGCTCGATCTGAGTTTGAGGGTTTGCTTGAGAAAACTCCAGCGGAAGTGAATGCATATTTATCCAAGCCAAGTGAATATACCGCTGCAATGAGTAATGCTGGGGATGCACAGGCCAGGGACACCTTGGAGCGTGTTCTTGAGTGCCTTTCCAGAGAAAGATGCGAAACATTCCAAGATTGTATTGCCTGGGCGCGCCTCAAGTTTGAAGATTATTTTTCCGACCGGGTGAAACAGCTGACATATACTTTCCCTGAAGATGCTGCAACTAGTACTGGGGCTCCATTCTGGTCTGCCCCCAAGCGATTCCCCCGTGCACTGCAGTTCTCAGCTAATGATCCCGGTCATCTCCATTTTGTTATGGCAGCATCCATACTCAGGGCAGAGACATTTGGGATCCCAATCCCCGACTGGGTTAGGAACTCTAAGAAGTTGTCTGAAGCTGTTGAGAAAGTGGAAGTCCCAGACTTTCAGCCCAAGAAAGATGCAAAAATAGTGACAGATGATAAGGCCACCAATTTAACTCCTCAATCTATAGATGATGCACAGGTTATTAATGAATTGATTATCAAGTTAGAGCATTGTCGAGAGAAACTCCCACCAGGGTTCCGGATGAAACCGATTCAATTCGAAAAGGTAACGCAATATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030054 GO:0032446 GO:0033554 GO:0036211 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 ko05012 map04120 map05012

Pfam Domains

Protein Families

E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

955

Amino Acids

105.03

Weight (kDa)

5.13

Isoelectric Point (pI)

31.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	147 - 522	1.4e-27	ThiF family
E1_FCCH	PF16190	321 - 391	6.8e-29	Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB	PF16191	392 - 462	3.4e-25	Ubiquitin-activating enzyme E1 four-helix bundle
ThiF	PF00899	544 - 786	2e-53	ThiF family
UBA_E1_SCCH	PF10585	731 - 951	1.2e-67	Ubiquitin-activating enzyme, SCCH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30110 AT5G06460
fragaria_vesca	FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_6g10930 FvH4_6g10930 FvH4_6g10930
malus_domestica	MD04G1163500.v1.1 MD11G1065700.v1.1 MD12G1177300.v1.1
prunus_persica	Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G282900_v2.0.a1
pyrus_communis	pycom04g14620 pycom11g05570 pycom12g16790
rosa_chinensis	RchiOBHm_Chr3g0462201 RchiOBHm_Chr5g0070911
rosa_laevigata	RLG00000024878 RLG00000036170
rosa_multiflora	Rmu_sc0002944.1_g000004 Rmu_sc0005970.1_g000009 Rmu_sc0006715.1_g000003
rosa_roxburghii	Rroxscaffold_1G00010290 Rroxscaffold_6G00417690
rosa_rugosa	Rorug03G0054900 Rorug05G0408500 Rorug05G0408500 Rorug05G0408500
rosa_samantha	Rh3CG119500 Rh5AG464600 Rh5DG494900
rosa_wichuraiana	Rw3G009590 Rw5G043220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	254, 1136
AccB1I	GGYRCC	5 cut(s)	49, 101, 254, 1136, 2135
AccI	GTMKAC	1 cut(s)	1865
AccII	CGCG	1 cut(s)	2413
AccIII	TCCGGA	1 cut(s)	2826
AciI	CCGC	8 cut(s)	266, 363, 734, 827, 1875, 2038, 2261, 2301
AclWI	GGATC	8 cut(s)	305, 441, 1026, 1711, 2141, 2549, 2608, 2621
AcoI	YGGCCR	2 cut(s)	321, 463
AcsI	RAATTY	5 cut(s)	797, 896, 1004, 1301, 1978
AcuI	CTGAAG	2 cut(s)	2493, 2676
AcyI	GRCGYC	2 cut(s)	50, 90
AfaI	GTAC	7 cut(s)	256, 516, 1138, 1464, 2077, 2176, 2491
AfiI	CCNNNNNNNGG	6 cut(s)	215, 935, 1279, 1334, 2595, 2629
AhlI	ACTAGT	1 cut(s)	2486
AjiI	CACGTC	1 cut(s)	385
AjnI	CCWGG	5 cut(s)	753, 2018, 2333, 2405, 2818
AleI	CACNNNNGTG	2 cut(s)	1166, 2170
Alw21I	GWGCWC	2 cut(s)	1714, 2536
Alw26I	GTCTC	5 cut(s)	199, 222, 464, 1910, 2597
Alw44I	GTGCAC	2 cut(s)	1710, 2532
AlwI	GGATC	8 cut(s)	305, 441, 1026, 1711, 2141, 2549, 2608, 2621
Aor13HI	TCCGGA	1 cut(s)	2826
AoxI	GGCC	7 cut(s)	321, 463, 520, 859, 1936, 2331, 2727
ApaLI	GTGCAC	2 cut(s)	1710, 2532
ApoI	RAATTY	5 cut(s)	797, 896, 1004, 1301, 1978
AscI	GGCGCGCC	1 cut(s)	2411
AseI	ATTAAT	3 cut(s)	1383, 1995, 2772
Asp700I	GAANNNNTTC	1 cut(s)	1578
Asp718I	GGTACC	2 cut(s)	254, 1136
AspLEI	GCGC	4 cut(s)	52, 1437, 2413, 2415
AspS9I	GGNCC	4 cut(s)	520, 890, 1656, 1937
AsuC2I	CCSGG	4 cut(s)	467, 1224, 2444, 2559
AsuHPI	GGTGA	6 cut(s)	157, 245, 1238, 1253, 2108, 2458
AsuII	TTCGAA	1 cut(s)	2847
AvaII	GGWCC	2 cut(s)	890, 1656
AxyI	CCTNAGG	1 cut(s)	1126
BaeGI	GKGCMC	3 cut(s)	661, 1714, 2536
BaeI	ACNNNNGTAYC	2 cut(s)	1128, 1161
BamHI	GGATCC	1 cut(s)	2613
BanI	GGYRCC	5 cut(s)	49, 101, 254, 1136, 2135
BanII	GRGCYC	2 cut(s)	2224, 2500
BarI	GAAGNNNNNNTAC	2 cut(s)	1563, 1595
Bbv12I	GWGCWC	2 cut(s)	1714, 2536
BccI	CCATC	7 cut(s)	332, 911, 1007, 1058, 1394, 1741, 2098
BceAI	ACGGC	2 cut(s)	440, 1611
BciT130I	CCWGG	5 cut(s)	755, 2020, 2335, 2407, 2820
BciVI	GTATCC	1 cut(s)	1114
BclI	TGATCA	4 cut(s)	282, 1258, 2032, 2203
BcnI	CCSGG	4 cut(s)	467, 1224, 2444, 2559
BcoDI	GTCTC	5 cut(s)	199, 222, 464, 1910, 2597
BcuI	ACTAGT	1 cut(s)	2486
BfaI	CTAG	5 cut(s)	198, 1092, 2003, 2142, 2487
BfmI	CTRYAG	5 cut(s)	39, 1664, 1866, 2537, 2751
BfoI	RGCGCY	1 cut(s)	53
BfuI	GTATCC	1 cut(s)	1114
BglI	GCCNNNNNGGC	1 cut(s)	320
BglII	AGATCT	1 cut(s)	1414
BmcAI	AGTACT	2 cut(s)	1464, 2491
Bme1390I	CCNGG	9 cut(s)	467, 755, 1224, 2020, 2335, 2407, 2444, 2559, 2820
Bme18I	GGWCC	2 cut(s)	890, 1656
BmgBI	CACGTC	1 cut(s)	385
BmgT120I	GGNCC	4 cut(s)	520, 890, 1656, 1937
BmrFI	CCNGG	9 cut(s)	467, 755, 1224, 2020, 2335, 2407, 2444, 2559, 2820
BmrI	ACTGGG	4 cut(s)	1403, 1981, 2502, 2638
BmuI	ACTGGG	4 cut(s)	1403, 1981, 2502, 2638
BoxI	GACNNNNGTC	1 cut(s)	810
BplI	GAGNNNNNCTC	4 cut(s)	1109, 1141, 2796, 2828
BpmI	CTGGAG	3 cut(s)	1697, 2136, 2241
Bpu14I	TTCGAA	1 cut(s)	2847
BpuEI	CTTGAG	6 cut(s)	1133, 1199, 2087, 2267, 2378, 2402
BpuMI	CCSGG	4 cut(s)	467, 1224, 2444, 2559
Bsa29I	ATCGAT	1 cut(s)	426
BsaBI	GATNNNNATC	4 cut(s)	432, 1017, 1419, 2618
BsaHI	GRCGYC	2 cut(s)	50, 90
BsaI	GGTCTC	1 cut(s)	199
BsaJI	CCNNGG	8 cut(s)	523, 1073, 1473, 1768, 2334, 2343, 2406, 2819
BsaWI	WCCGGW	2 cut(s)	121, 2826
BsaXI	ACNNNNNCTCC	2 cut(s)	2482, 2512
Bsc4I	CCNNNNNNNGG	6 cut(s)	215, 935, 1279, 1334, 2595, 2629
Bse118I	RCCGGY	1 cut(s)	444
Bse21I	CCTNAGG	1 cut(s)	1126
Bse3DI	GCAATG	2 cut(s)	964, 2313
Bse8I	GATNNNNATC	4 cut(s)	432, 1017, 1419, 2618
BseAI	TCCGGA	1 cut(s)	2826
BseBI	CCWGG	5 cut(s)	755, 2020, 2335, 2407, 2820
BseCI	ATCGAT	1 cut(s)	426
BseDI	CCNNGG	8 cut(s)	523, 1073, 1473, 1768, 2334, 2343, 2406, 2819
BseGI	GGATG	9 cut(s)	491, 643, 1018, 1354, 1537, 1724, 2329, 2585, 2835
BseJI	GATNNNNATC	4 cut(s)	432, 1017, 1419, 2618
BseLI	CCNNNNNNNGG	6 cut(s)	215, 935, 1279, 1334, 2595, 2629
BseMI	GCAATG	2 cut(s)	964, 2313
BsePI	GCGCGC	1 cut(s)	2411
BseRI	GAGGAG	4 cut(s)	493, 1130, 1257, 2733
BseSI	GKGCMC	3 cut(s)	661, 1714, 2536
BseYI	CCCAGC	1 cut(s)	2318
BsgI	GTGCAG	2 cut(s)	167, 422
Bsh1236I	CGCG	1 cut(s)	2413
Bsh1285I	CGRYCG	1 cut(s)	2443
BshFI	GGCC	7 cut(s)	323, 465, 522, 861, 1938, 2333, 2729
BshNI	GGYRCC	5 cut(s)	49, 101, 254, 1136, 2135
BshVI	ATCGAT	1 cut(s)	426
BsiEI	CGRYCG	1 cut(s)	2443
BsiHKAI	GWGCWC	2 cut(s)	1714, 2536
BsiSI	CCGG	9 cut(s)	87, 122, 417, 445, 466, 1224, 2443, 2559, 2827
BslFI	GGGAC	5 cut(s)	604, 1332, 1455, 2351, 2663
BslI	CCNNNNNNNGG	6 cut(s)	215, 935, 1279, 1334, 2595, 2629
BsmAI	GTCTC	5 cut(s)	199, 222, 464, 1910, 2597
BsmBI	CGTCTC	3 cut(s)	222, 464, 1910
BsmFI	GGGAC	5 cut(s)	604, 1332, 1455, 2351, 2663
BsmI	GAATGC	1 cut(s)	2275
BsnI	GGCC	7 cut(s)	323, 465, 522, 861, 1938, 2333, 2729
Bso31I	GGTCTC	1 cut(s)	199
Bsp119I	TTCGAA	1 cut(s)	2847
Bsp1286I	GDGCHC	5 cut(s)	661, 1714, 2224, 2500, 2536
Bsp13I	TCCGGA	1 cut(s)	2826
Bsp19I	CCATGG	1 cut(s)	1473
BspACI	CCGC	8 cut(s)	266, 363, 734, 827, 1875, 2038, 2261, 2301
BspANI	GGCC	7 cut(s)	323, 465, 522, 861, 1938, 2333, 2729
BspDI	ATCGAT	1 cut(s)	426
BspEI	TCCGGA	1 cut(s)	2826
BspFNI	CGCG	1 cut(s)	2413
BspMAI	CTGCAG	1 cut(s)	2541
BspPI	GGATC	8 cut(s)	305, 441, 1026, 1711, 2141, 2549, 2608, 2621
BspT104I	TTCGAA	1 cut(s)	2847
BspT107I	GGYRCC	5 cut(s)	49, 101, 254, 1136, 2135
BspTNI	GGTCTC	1 cut(s)	199
BsrDI	GCAATG	2 cut(s)	964, 2313
BsrFI	RCCGGY	1 cut(s)	444
BssAI	RCCGGY	1 cut(s)	444
BssECI	CCNNGG	8 cut(s)	523, 1073, 1473, 1768, 2334, 2343, 2406, 2819
BssHII	GCGCGC	1 cut(s)	2411
BssNI	GRCGYC	2 cut(s)	50, 90
BssT1I	CCWWGG	4 cut(s)	1073, 1473, 1768, 2343
Bst2UI	CCWGG	5 cut(s)	755, 2020, 2335, 2407, 2820
Bst4CI	ACNGT	8 cut(s)	101, 233, 359, 595, 904, 1870, 2179, 2210
Bst6I	CTCTTC	3 cut(s)	1338, 1550, 1802
BstACI	GRCGYC	2 cut(s)	50, 90
BstAPI	GCANNNNNTGC	1 cut(s)	190
BstBI	TTCGAA	1 cut(s)	2847
BstC8I	GCNNGC	7 cut(s)	446, 453, 1406, 1522, 1683, 1857, 2413
BstDSI	CCRYGG	1 cut(s)	1473
BstENI	CCTNNNNNAGG	1 cut(s)	213
BstF5I	GGATG	9 cut(s)	491, 643, 1018, 1354, 1537, 1724, 2329, 2585, 2835
BstFNI	CGCG	1 cut(s)	2413
BstH2I	RGCGCY	1 cut(s)	53
BstHHI	GCGC	4 cut(s)	52, 1437, 2413, 2415
BstMAI	GTCTC	5 cut(s)	199, 222, 464, 1910, 2597
BstMCI	CGRYCG	1 cut(s)	2443
BstNI	CCWGG	5 cut(s)	755, 2020, 2335, 2407, 2820
BstNSI	RCATGY	1 cut(s)	1685
BstPAI	GACNNNNGTC	1 cut(s)	810
BstSCI	CCNGG	9 cut(s)	465, 753, 1222, 2018, 2333, 2405, 2442, 2557, 2818
BstSFI	CTRYAG	5 cut(s)	39, 1664, 1866, 2537, 2751
BstSLI	GKGCMC	3 cut(s)	661, 1714, 2536
BstUI	CGCG	1 cut(s)	2413
BstX2I	RGATCY	6 cut(s)	433, 1018, 1414, 1703, 2146, 2613
BstYI	RGATCY	6 cut(s)	433, 1018, 1414, 1703, 2146, 2613
Bsu15I	ATCGAT	1 cut(s)	426
Bsu36I	CCTNAGG	1 cut(s)	1126
BsuI	GTATCC	1 cut(s)	1114
BsuRI	GGCC	7 cut(s)	323, 465, 522, 861, 1938, 2333, 2729
BsuTUI	ATCGAT	1 cut(s)	426
BtgI	CCRYGG	1 cut(s)	1473
BtrI	CACGTC	1 cut(s)	385
BtsCI	GGATG	9 cut(s)	491, 643, 1018, 1354, 1537, 1724, 2329, 2585, 2835
BtsI	GCAGTG	1 cut(s)	2534
BtsIMutI	CAGTG	9 cut(s)	249, 768, 900, 1292, 1592, 1609, 1785, 2206, 2534
Cac8I	GCNNGC	7 cut(s)	446, 453, 1406, 1522, 1683, 1857, 2413
CfoI	GCGC	4 cut(s)	52, 1437, 2413, 2415
Cfr10I	RCCGGY	1 cut(s)	444
Cfr13I	GGNCC	4 cut(s)	520, 890, 1656, 1937
ClaI	ATCGAT	1 cut(s)	426
CseI	GACGC	2 cut(s)	79, 264
Csp6I	GTAC	7 cut(s)	255, 515, 1137, 1463, 2076, 2175, 2490
CviQI	GTAC	7 cut(s)	255, 515, 1137, 1463, 2076, 2175, 2490
DinI	GGCGCC	1 cut(s)	51
DraI	TTTAAA	1 cut(s)	18
EaeI	YGGCCR	2 cut(s)	321, 463
Eam1104I	CTCTTC	3 cut(s)	1338, 1550, 1802
EarI	CTCTTC	3 cut(s)	1338, 1550, 1802
Eco130I	CCWWGG	4 cut(s)	1073, 1473, 1768, 2343
Eco147I	AGGCCT	1 cut(s)	861
Eco24I	GRGCYC	2 cut(s)	2224, 2500
Eco31I	GGTCTC	1 cut(s)	199
Eco32I	GATATC	2 cut(s)	424, 775
Eco47I	GGWCC	2 cut(s)	890, 1656
Eco57I	CTGAAG	2 cut(s)	2493, 2676
Eco81I	CCTNAGG	1 cut(s)	1126
EcoNI	CCTNNNNNAGG	1 cut(s)	213
EcoO109I	RGGNCCY	1 cut(s)	520
EcoRI	GAATTC	2 cut(s)	797, 896
EcoRII	CCWGG	5 cut(s)	753, 2018, 2333, 2405, 2818
EcoRV	GATATC	2 cut(s)	424, 775
EcoT14I	CCWWGG	4 cut(s)	1073, 1473, 1768, 2343
EcoT22I	ATGCAT	1 cut(s)	2275
EcoT38I	GRGCYC	2 cut(s)	2224, 2500
EgeI	GGCGCC	1 cut(s)	51
EheI	GGCGCC	1 cut(s)	51
ErhI	CCWWGG	4 cut(s)	1073, 1473, 1768, 2343
Esp3I	CGTCTC	3 cut(s)	222, 464, 1910
FaqI	GGGAC	5 cut(s)	604, 1332, 1455, 2351, 2663
FauI	CCCGC	1 cut(s)	356
FbaI	TGATCA	4 cut(s)	282, 1258, 2032, 2203
FblI	GTMKAC	1 cut(s)	1865
FokI	GGATG	9 cut(s)	478, 650, 1025, 1361, 1524, 1731, 2336, 2572, 2842
FriOI	GRGCYC	2 cut(s)	2224, 2500
FspBI	CTAG	5 cut(s)	198, 1092, 2003, 2142, 2487
GlaI	GCGC	4 cut(s)	51, 1436, 2412, 2414
GsaI	CCCAGC	1 cut(s)	2322
GsuI	CTGGAG	3 cut(s)	1697, 2136, 2241
HaeII	RGCGCY	1 cut(s)	53
HaeIII	GGCC	7 cut(s)	323, 465, 522, 861, 1938, 2333, 2729
HapII	CCGG	9 cut(s)	87, 122, 417, 445, 466, 1224, 2443, 2559, 2827
HgaI	GACGC	2 cut(s)	79, 264
HhaI	GCGC	4 cut(s)	52, 1437, 2413, 2415
Hin1I	GRCGYC	2 cut(s)	50, 90
Hin6I	GCGC	4 cut(s)	50, 1435, 2411, 2413
HinP1I	GCGC	4 cut(s)	50, 1435, 2411, 2413
HincII	GTYRAC	1 cut(s)	241
HindII	GTYRAC	1 cut(s)	241
HindIII	AAGCTT	3 cut(s)	290, 1358, 1518
HpaI	GTTAAC	1 cut(s)	241
HpaII	CCGG	9 cut(s)	87, 122, 417, 445, 466, 1224, 2443, 2559, 2827
HphI	GGTGA	6 cut(s)	157, 245, 1238, 1253, 2108, 2458
Hpy166II	GTNNAC	6 cut(s)	241, 1712, 1866, 2175, 2182, 2534
Hpy8I	GTNNAC	6 cut(s)	241, 1712, 1866, 2175, 2182, 2534
Hpy99I	CGWCG	4 cut(s)	95, 101, 155, 386
HpyAV	CCTTC	5 cut(s)	209, 218, 581, 847, 2050
HpyCH4III	ACNGT	8 cut(s)	101, 233, 359, 595, 904, 1870, 2179, 2210
HpyCH4IV	ACGT	3 cut(s)	237, 309, 384
HpySE526I	ACGT	3 cut(s)	237, 309, 384
Hsp92I	GRCGYC	2 cut(s)	50, 90
HspAI	GCGC	4 cut(s)	50, 1435, 2411, 2413
KasI	GGCGCC	1 cut(s)	49
Kpn2I	TCCGGA	1 cut(s)	2826
KpnI	GGTACC	2 cut(s)	258, 1140
KroI	GCCGGC	1 cut(s)	444
KroNI	GCCGGC	1 cut(s)	446
Ksp22I	TGATCA	4 cut(s)	282, 1258, 2032, 2203
KspAI	GTTAAC	1 cut(s)	241
LmnI	GCTCC	7 cut(s)	366, 598, 736, 1588, 1678, 2347, 2503
MaeI	CTAG	5 cut(s)	198, 1092, 2003, 2142, 2487
MaeII	ACGT	3 cut(s)	237, 309, 384
MaeIII	GTNAC	9 cut(s)	233, 338, 571, 779, 881, 1159, 1811, 2713, 2854
MflI	RGATCY	6 cut(s)	433, 1018, 1414, 1703, 2146, 2613
MhlI	GDGCHC	5 cut(s)	661, 1714, 2224, 2500, 2536
Mly113I	GGCGCC	1 cut(s)	50
MlyI	GAGTC	2 cut(s)	1559, 2078
MmeI	TCCRAC	5 cut(s)	43, 435, 1607, 1681, 2463
Mph1103I	ATGCAT	1 cut(s)	2275
MroI	TCCGGA	1 cut(s)	2826
MroNI	GCCGGC	1 cut(s)	444
MroXI	GAANNNNTTC	1 cut(s)	1578
MseI	TTAA	9 cut(s)	17, 240, 1383, 1419, 1619, 1982, 1995, 2738, 2772
MslI	CAYNNNNRTG	4 cut(s)	1166, 1717, 2102, 2170
MspA1I	CMGCKG	4 cut(s)	2038, 2261, 2303, 2455
MspI	CCGG	9 cut(s)	87, 122, 417, 445, 466, 1224, 2443, 2559, 2827
MspR9I	CCNGG	9 cut(s)	467, 755, 1224, 2020, 2335, 2407, 2444, 2559, 2820
Mva1269I	GAATGC	1 cut(s)	2275
MvaI	CCWGG	5 cut(s)	755, 2020, 2335, 2407, 2820
MvnI	CGCG	1 cut(s)	2413
NaeI	GCCGGC	1 cut(s)	446
NarI	GGCGCC	1 cut(s)	50
NciI	CCSGG	4 cut(s)	467, 1224, 2444, 2559
NcoI	CCATGG	1 cut(s)	1473
NgoMIV	GCCGGC	1 cut(s)	444
NmeAIII	GCCGAG	1 cut(s)	242
NmuCI	GTSAC	5 cut(s)	233, 571, 881, 1159, 2713
NsiI	ATGCAT	1 cut(s)	2275
NspI	RCATGY	1 cut(s)	1685
NspV	TTCGAA	1 cut(s)	2847
OliI	CACNNNNGTG	2 cut(s)	1166, 2170
PaeI	GCATGC	1 cut(s)	1685
PalAI	GGCGCGCC	1 cut(s)	2411
PauI	GCGCGC	1 cut(s)	2411
PceI	AGGCCT	1 cut(s)	861
PcsI	WCGNNNNNNNCGW	1 cut(s)	306
PctI	GAATGC	1 cut(s)	2275
PdiI	GCCGGC	1 cut(s)	446
PdmI	GAANNNNTTC	1 cut(s)	1578
PleI	GAGTC	2 cut(s)	1559, 2077
PluTI	GGCGCC	1 cut(s)	53
PpsI	GAGTC	2 cut(s)	1559, 2077
PshAI	GACNNNNGTC	1 cut(s)	810
PshBI	ATTAAT	3 cut(s)	1383, 1995, 2772
Psp6I	CCWGG	5 cut(s)	753, 2018, 2333, 2405, 2818
PspFI	CCCAGC	1 cut(s)	2318
PspGI	CCWGG	5 cut(s)	753, 2018, 2333, 2405, 2818
PspPI	GGNCC	4 cut(s)	520, 890, 1656, 1937
PstI	CTGCAG	1 cut(s)	2541
PsuI	RGATCY	6 cut(s)	433, 1018, 1414, 1703, 2146, 2613
PteI	GCGCGC	1 cut(s)	2411
PvuII	CAGCTG	1 cut(s)	2455
RsaI	GTAC	7 cut(s)	256, 516, 1138, 1464, 2077, 2176, 2491
RsaNI	GTAC	7 cut(s)	255, 515, 1137, 1463, 2076, 2175, 2490
RseI	CAYNNNNRTG	4 cut(s)	1166, 1717, 2102, 2170
SaqAI	TTAA	9 cut(s)	17, 240, 1383, 1419, 1619, 1982, 1995, 2738, 2772
Sau96I	GGNCC	4 cut(s)	520, 890, 1656, 1937
ScaI	AGTACT	2 cut(s)	1464, 2491
SchI	GAGTC	2 cut(s)	1559, 2078
ScrFI	CCNGG	9 cut(s)	467, 755, 1224, 2020, 2335, 2407, 2444, 2559, 2820
SduI	GDGCHC	5 cut(s)	661, 1714, 2224, 2500, 2536
SfcI	CTRYAG	5 cut(s)	39, 1664, 1866, 2537, 2751
SfoI	GGCGCC	1 cut(s)	51
SfuI	TTCGAA	1 cut(s)	2847
SgsI	GGCGCGCC	1 cut(s)	2411
SinI	GGWCC	2 cut(s)	890, 1656
SmiMI	CAYNNNNRTG	4 cut(s)	1166, 1717, 2102, 2170
SmlI	CTYRAG	6 cut(s)	1112, 1214, 2066, 2246, 2357, 2417
SmoI	CTYRAG	6 cut(s)	1112, 1214, 2066, 2246, 2357, 2417
SpeI	ACTAGT	1 cut(s)	2486
SphI	GCATGC	1 cut(s)	1685
SseBI	AGGCCT	1 cut(s)	861
SsiI	CCGC	8 cut(s)	266, 363, 734, 827, 1875, 2038, 2261, 2301
SspDI	GGCGCC	1 cut(s)	49
SspI	AATATT	1 cut(s)	2864
SspMI	CTAG	5 cut(s)	198, 1092, 2003, 2142, 2487
StuI	AGGCCT	1 cut(s)	861
StyD4I	CCNGG	9 cut(s)	465, 753, 1222, 2018, 2333, 2405, 2442, 2557, 2818
StyI	CCWWGG	4 cut(s)	1073, 1473, 1768, 2343
TaaI	ACNGT	8 cut(s)	101, 233, 359, 595, 904, 1870, 2179, 2210
TaiI	ACGT	3 cut(s)	240, 312, 387
TaqI	TCGA	6 cut(s)	300, 426, 987, 2224, 2803, 2847
TatI	WGTACW	3 cut(s)	1462, 2174, 2489
TauI	GCSGC	3 cut(s)	269, 829, 1877
Tru1I	TTAA	9 cut(s)	17, 240, 1383, 1419, 1619, 1982, 1995, 2738, 2772
Tru9I	TTAA	9 cut(s)	17, 240, 1383, 1419, 1619, 1982, 1995, 2738, 2772
TscAI	CASTG	9 cut(s)	256, 775, 907, 1299, 1599, 1609, 1792, 2213, 2541
TseFI	GTSAC	5 cut(s)	233, 571, 881, 1159, 2713
Tsp45I	GTSAC	5 cut(s)	233, 571, 881, 1159, 2713
TspGWI	ACGGA	2 cut(s)	1601, 1826
TspRI	CASTG	9 cut(s)	256, 775, 907, 1299, 1599, 1609, 1792, 2213, 2541
VneI	GTGCAC	2 cut(s)	1710, 2532
VpaK11BI	GGWCC	2 cut(s)	890, 1656
VspI	ATTAAT	3 cut(s)	1383, 1995, 2772
XagI	CCTNNNNNAGG	1 cut(s)	213
XapI	RAATTY	5 cut(s)	797, 896, 1004, 1301, 1978
XbaI	TCTAGA	1 cut(s)	2141
XceI	RCATGY	1 cut(s)	1685
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1445, 1693, 2341, 2625
XmiI	GTMKAC	1 cut(s)	1865
XmnI	GAANNNNTTC	1 cut(s)	1578
XspI	CTAG	5 cut(s)	198, 1092, 2003, 2142, 2487
ZrmI	AGTACT	2 cut(s)	1464, 2491
Zsp2I	ATGCAT	1 cut(s)	2275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.