MD04G1163500.v1.1

Ubiquitin-activating enzyme E1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002217
Physical Location & Seq
Reverse (-)
25479404 .. 25485616
6213 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1163500.v1.1.491

Sequence Viewer

Length: 3276 bp
ATGAGATTTTGGTGTTCCGGCAGTTCATCGCACTATATGCTTCCTAGAAAGAGAGAAGCAGTGGGCGGAGAGGTTGTAGTAAACGAAGCCACGGAATCTCCGATCAAGAAGCCTCGCGGAGCTGCGACTACTGACGATTCTAAGAGCAACGAAAACAAAAACACCACCACCTTTAACAACAACAGCAACAGCAGTAGCAGCATCGAAGAATCTCCAATCATGGCATTGGGCAACGGGAGCTCAAACGATATCGATGAAGATCTCCACAGCCGGCAGTTGGCGGTGTATGGCAGGGAGACCATGCGCCGGCTCTTTGCCTCCAACATTCTCGTCTCGGGGATGCAGGGCTTGGGCGCTGAGATTGCAAAAAACCTTGTTCTTGCCGGTGTCAAGTCTGTGACTTTGCATGATGATGGAGTTGTTGAGTTGTGGGACTTGTCAAGCAACTTCTTCTTTTCTGAGGAGGATGTTGGAAAGAATCGGGCCCTTGCCTGTGTTCAAAAATTACAAGAACTAAACAACGCTGTTGTCATTTCGACCATAACAACTGAACTGACTAAAGAGAAACTCTCTGATTTCCAGGCTGTAGTTTTCACTGATATTAGCTTGGAGAAGGCAATTGAATTCGATGACTACTGCCATAATCATAACCCTCCCATCTCTTTTATAAAATCAGAAGTACGAGGCCTTTTTGGCAGCGTATTTTGTGACTTTGGCCCTGAGTTCACTGTTCTTGATGTTGATGGTGAGGATCCGCATACAGGTATAATTGCATCGATTAGTAATGACAACCCTGCTCTCATAGCATGTGTTGATGATGAGAGGCTTGAGTTCCAGGATGGGGATTTAGTTGTGTTCACCGAAGTCCATGGAATGACCGAGTTGAATGATGGAAAGCCGAGGAAGGTTAAGAATGCAAGACCTTACTCATTCACAATCGAGGAGGACACTACCAATTATGCTGCATATGAGAAAGGGGGTATAGTCTCACAGGTGAAGCAACCCAAGGTGTTGAACTTTAAGCCTCTGCGAGAAGCACTCAAGGATCATGGTGACTTCCTTCTGAGTGACTTCTCCAAGTTTGATCGCCCACCTCTCCTACACTTGGCATTTCAGGCACTAGATAAGTTCATTTCAGAGTTGGGGCGTTTCCCTGTTGCTGGATCTGAGGATGATGCTACAAAATTCATATCTATGGTAACTAACATCAATGATAGCTCAGCAGATGGTAAGCTTGAGGAGATTGACCACAAGGTTCTTCGTCATTTTGCCTTTGGTGCTAGAGCTGTGCTAAATCCCATGGCTGCTATGTTTGGTGGTATTGTTGGACAAGAAGTTGTGAAGGCCTGTTCTGCTAAGTTCCATCCTCTTTTCCAGTTTTTCTACTTCGATTCAGTTGAATCCCTTCCTTCAGAGACCTTGGATCCTAATGATTTGAAGCCTTTGAATAGCCGATATGATGCACAAATTTCAGTATTTGGAGCCAAGCTCCAGAAAAAGCTGGAGGACACAAAAGTGTTCACTGTCGGATCTGGGGCACTAGGATGTGAGTTTTTGAAGAATTTAGCATTGATGGGTGTCTCTTGTGGTAAAGAAGGGAAGTTAACAATAACAGATGATGATGTCATTGAGAAGAGTAACCTTAGCAGGCAATTTCTCTTTAGAGATTGGAACATTGGGCAGGCTAAATCAACAGTAGCTGCCTCTGCTGCTATGGTGATAAATGGTCGTCTGAACATTGAAGCACTGCAGAATCGTGCAAGCCCAGATACTGAAAATGTGTTTGATGATACTTTTTGGGAGAATTTGGATGTTGTTATCAATGCTCTGGATAATGTGAATGCAAGGCTTTACATTGATCAGAGATGCTTGTACTTCCAGAAGCCACTTTTGGAGTCAGGAACTCTAGGTGCCAAGTGCAACACGCAGATGGTCATTCCTCACCTGACTGAAAATTATGGAGCATCACGGGACCCACCTGAAAAGCAAGCACCCATGTGCACAGTTCATTCATTCCCTCACAACATTGACCATTGCTTGACATGGGCCCGGTCTGAGTTTGAGGGTCTACTTGAGAAGGTGCCAGCTGAAGTAAATGCCTACCTGACTAATCCTAATGAATACATTGCCGCAATGAAAAATGCTGGTGATGCTCAGGCCAGGAACAACTTGGAAAGTGTCATTGAATGCCTTGACAAAGAGAGATGTGAGACGTTCCAAGATTGCATAAGCTGGGCCCGTCTGAAGTTTGAGGACTACTTTGCTAACCGTGTGAAGCAGTTAACTTATACTTTTCCTGAGGATGCTACAACCAGTAGTGGGACACCATTTTGGTCTGCCCCCAAGCGTTTTCCTCGCCCACTGCAGTTCTCAGTTGATGATCTCAGCCACCTCCAGTTTTTAATGGCAGCATCTATACTACGAGCAGAGACATTCAACATTCCAATTCCTGATTGGGTCAAGTCTCGTGCAAAGTTTGCTGATGCTGTTAACAAAGTGATGGTACCGGATTTTCAGCCTAAGAAAGATGTGAAGATTGAGACTGATGAGAAAGCTACAACCGTCTTACCAGCATCGATTGATGATGCTGCGGTTATCAACGAGTTAGTTGTGAAGTTAGAAAGATGCAAGGAGCGGCTGCCACCAGGCTTCAAGATGAACCCGATTCAGTTTGAGAAGGATGATGATACAAACTATCATATGGACCTAATAGCTGGATTTGCAAATATGAGGGCAAGGAACTATGGCATTGGTGAAGTCGACAAACTGAAAGCCAAATTCATTGCAGGAAGAATCATTCCTGCAATTGCAACATCAACTGCTCTGGCAACCGGCCTTGTTTGCTTGGAGCTGTACAAGGTTCTGGACGGAGGGCACAAGGTTGAAGACTACCGAAACACCTTTGCCAATCTCTCCCTTCCTCTGTTCTCCATGGCTGAACCAGTCCCTCCTAAAGTCATCAAGCACCAAGATATGAAGTGGACAGTATGGGACAGGTGGATTATAAAGGACAACCCAACTTTAAAGCAGCTCCTCAAGTGGCTCGAAGACCAGGGCTTAAACGCGTACAGCATCTCCTATGGAAGTTGCCTGCTCTTTAACAGCATGTTCCCGAAGCACAAGGAGCGCATGGACAGGACCATGGTGGATTTGGCTACGAGTATTGCGAAGGCAGAACTGCCTGCTAATAGGAAGCACTTTGACGTGGTGGTGGCTTGCGAAGATGAAGAAGAGAACGATATTGATATCCCGCAAATCTCCATTTACTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko05012 map04120 map05012
Pfam Domains
Protein Families
E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1092

Amino Acids

121.93

Weight (kDa)

5.14

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 89 - 463 4.6e-25 ThiF family
E1_FCCH PF16190 263 - 333 2.1e-27 Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB PF16191 334 - 404 3e-24 Ubiquitin-activating enzyme E1 four-helix bundle
ThiF PF00899 486 - 978 6.4e-60 ThiF family
UBA_E1_SCCH PF10585 673 - 926 7.7e-86 Ubiquitin-activating enzyme, SCCH domain
E1_UFD PF09358 997 - 1086 1.4e-23 Ubiquitin fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 668, 3005
Acc65I GGTACC 1 cut(s) 2503
AccB1I GGYRCC 3 cut(s) 1910, 2080, 2503
AccBSI CCGCTC 1 cut(s) 2635
AccI GTMKAC 2 cut(s) 2068, 2760
AccII CGCG 2 cut(s) 117, 3065
AciI CCGC 8 cut(s) 66, 117, 281, 755, 2130, 2591, 2635, 3251
AclWI GGATC 7 cut(s) 746, 759, 1053, 1171, 1418, 1431, 1537
AcsI RAATTY 6 cut(s) 623, 1184, 1467, 1561, 1804, 2777
AcuI CTGAAG 3 cut(s) 1395, 2109, 2264
AfaI GTAC 5 cut(s) 681, 1874, 2505, 2855, 3068
AfiI CCNNNNNNNGG 7 cut(s) 277, 306, 761, 841, 1105, 1160, 2319
AflIII ACRYGT 1 cut(s) 3063
AjiI CACGTC 1 cut(s) 3205
AjnI CCWGG 5 cut(s) 579, 834, 2159, 2644, 3051
AjuI GAANNNNNNNTTGG 2 cut(s) 1874, 1906
AleI CACNNNNGTG 2 cut(s) 1514, 1996
Alw21I GWGCWC 2 cut(s) 242, 2003
Alw26I GTCTC 9 cut(s) 290, 337, 991, 1409, 1585, 2204, 2423, 2469, 2534
Alw44I GTGCAC 1 cut(s) 1999
AlwI GGATC 7 cut(s) 746, 759, 1053, 1171, 1418, 1431, 1537
AlwNI CAGNNNCTG 2 cut(s) 1700, 1772
Ama87I CYCGRG 1 cut(s) 334
AoxI GGCC 8 cut(s) 483, 685, 715, 1344, 2046, 2157, 2235, 2833
ApaI GGGCCC 3 cut(s) 487, 2050, 2239
ApaLI GTGCAC 1 cut(s) 1999
ApoI RAATTY 6 cut(s) 623, 1184, 1467, 1561, 1804, 2777
Asp700I GAANNNNTTC 1 cut(s) 1404
Asp718I GGTACC 1 cut(s) 2503
AspLEI GCGC 3 cut(s) 306, 356, 3129
AsuC2I CCSGG 1 cut(s) 2050
AsuHPI GGTGA 8 cut(s) 758, 850, 1006, 1064, 1729, 1934, 2159, 2765
AvaI CYCGRG 1 cut(s) 334
AvaII GGWCC 3 cut(s) 1972, 2704, 3138
AxyI CCTNAGG 1 cut(s) 2298
BaeGI GKGCMC 6 cut(s) 487, 1540, 2003, 2050, 2239, 2877
BamHI GGATCC 2 cut(s) 751, 1423
BanI GGYRCC 3 cut(s) 1910, 2080, 2503
BanII GRGCYC 4 cut(s) 242, 487, 2050, 2239
BauI CACGAG 1 cut(s) 2466
BbsI GAAGAC 2 cut(s) 2892, 3054
Bbv12I GWGCWC 2 cut(s) 242, 2003
BciT130I CCWGG 5 cut(s) 581, 836, 2161, 2646, 3053
BclI TGATCA 1 cut(s) 1858
BcnI CCSGG 1 cut(s) 2050
BcoDI GTCTC 9 cut(s) 290, 337, 991, 1409, 1585, 2204, 2423, 2469, 2534
BfaI CTAG 5 cut(s) 45, 1121, 1281, 1541, 1907
BfmI CTRYAG 3 cut(s) 585, 1748, 2363
BfoI RGCGCY 1 cut(s) 357
BglI GCCNNNNNGGC 1 cut(s) 693
BglII AGATCT 1 cut(s) 259
BlpI GCTNAGC 1 cut(s) 1219
Bme1390I CCNGG 6 cut(s) 581, 836, 2050, 2161, 2646, 3053
Bme18I GGWCC 3 cut(s) 1972, 2704, 3138
BmeT110I CYCGRG 1 cut(s) 334
BmgBI CACGTC 1 cut(s) 3205
BmrFI CCNGG 6 cut(s) 581, 836, 2050, 2161, 2646, 3053
BpiI GAAGAC 2 cut(s) 2892, 3054
BplI GAGNNNNNCTC 6 cut(s) 554, 586, 1023, 1055, 1473, 1505
BpmI CTGGAG 3 cut(s) 1475, 1523, 2378
Bpu10I CCTNAGC 2 cut(s) 1643, 2154
Bpu1102I GCTNAGC 1 cut(s) 1219
BpuEI CTTGAG 5 cut(s) 848, 1025, 1256, 2093, 3020
BpuMI CCSGG 1 cut(s) 2050
Bsa29I ATCGAT 3 cut(s) 252, 776, 2576
BsaBI GATNNNNATC 1 cut(s) 258
BsaI GGTCTC 2 cut(s) 290, 1409
BsaJI CCNNGG 9 cut(s) 90, 868, 899, 1005, 1299, 1419, 2931, 3052, 3141
BsaWI WCCGGW 1 cut(s) 2506
BsaXI ACNNNNNCTCC 6 cut(s) 60, 82, 90, 112, 3059, 3089
Bsc4I CCNNNNNNNGG 7 cut(s) 277, 306, 761, 841, 1105, 1160, 2319
Bse118I RCCGGY 4 cut(s) 270, 306, 383, 2831
Bse1I ACTGG 4 cut(s) 1375, 2313, 2395, 2942
Bse21I CCTNAGG 1 cut(s) 2298
Bse3DI GCAATG 4 cut(s) 2032, 2124, 2139, 2781
Bse8I GATNNNNATC 1 cut(s) 258
BseBI CCWGG 5 cut(s) 581, 836, 2161, 2646, 3053
BseCI ATCGAT 3 cut(s) 252, 776, 2576
BseDI CCNNGG 9 cut(s) 90, 868, 899, 1005, 1299, 1419, 2931, 3052, 3141
BseGI GGATG 9 cut(s) 345, 472, 844, 1177, 1363, 1550, 1816, 2308, 2686
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 7 cut(s) 277, 306, 761, 841, 1105, 1160, 2319
BseMI GCAATG 4 cut(s) 2032, 2124, 2139, 2781
BseNI ACTGG 4 cut(s) 1375, 2313, 2395, 2942
BseRI GAGGAG 4 cut(s) 476, 956, 1253, 3023
BseSI GKGCMC 6 cut(s) 487, 1540, 2003, 2050, 2239, 2877
BseYI CCCAGC 1 cut(s) 2232
Bsh1236I CGCG 2 cut(s) 117, 3065
BshFI GGCC 8 cut(s) 485, 687, 717, 1346, 2048, 2159, 2237, 2835
BshNI GGYRCC 3 cut(s) 1910, 2080, 2503
BshVI ATCGAT 3 cut(s) 252, 776, 2576
BsiHKAI GWGCWC 2 cut(s) 242, 2003
BsiHKCI CYCGRG 1 cut(s) 334
BsiSI CCGG 7 cut(s) 18, 271, 307, 384, 2050, 2507, 2832
BslFI GGGAC 5 cut(s) 446, 1985, 2335, 2930, 3005
BslI CCNNNNNNNGG 7 cut(s) 277, 306, 761, 841, 1105, 1160, 2319
BsmAI GTCTC 9 cut(s) 290, 337, 991, 1409, 1585, 2204, 2423, 2469, 2534
BsmBI CGTCTC 2 cut(s) 337, 2204
BsmFI GGGAC 5 cut(s) 446, 1985, 2335, 2930, 3005
BsmI GAATGC 3 cut(s) 919, 1846, 2192
BsnI GGCC 8 cut(s) 485, 687, 717, 1346, 2048, 2159, 2237, 2835
Bso31I GGTCTC 2 cut(s) 290, 1409
BsoBI CYCGRG 1 cut(s) 334
Bsp120I GGGCCC 3 cut(s) 483, 2046, 2235
Bsp1286I GDGCHC 7 cut(s) 242, 487, 1540, 2003, 2050, 2239, 2877
Bsp1407I TGTACA 1 cut(s) 2853
Bsp1720I GCTNAGC 1 cut(s) 1219
Bsp19I CCATGG 4 cut(s) 868, 1299, 2931, 3141
BspACI CCGC 8 cut(s) 66, 117, 281, 755, 2130, 2591, 2635, 3251
BspANI GGCC 8 cut(s) 485, 687, 717, 1346, 2048, 2159, 2237, 2835
BspDI ATCGAT 3 cut(s) 252, 776, 2576
BspFNI CGCG 2 cut(s) 117, 3065
BspMAI CTGCAG 2 cut(s) 1752, 2367
BspPI GGATC 7 cut(s) 746, 759, 1053, 1171, 1418, 1431, 1537
BspT107I GGYRCC 3 cut(s) 1910, 2080, 2503
BspTNI GGTCTC 2 cut(s) 290, 1409
BsrBI CCGCTC 1 cut(s) 2635
BsrDI GCAATG 4 cut(s) 2032, 2124, 2139, 2781
BsrFI RCCGGY 4 cut(s) 270, 306, 383, 2831
BsrGI TGTACA 1 cut(s) 2853
BsrI ACTGG 4 cut(s) 1375, 2313, 2395, 2942
BssAI RCCGGY 4 cut(s) 270, 306, 383, 2831
BssECI CCNNGG 9 cut(s) 90, 868, 899, 1005, 1299, 1419, 2931, 3052, 3141
BssSI CACGAG 1 cut(s) 2466
BssT1I CCWWGG 6 cut(s) 868, 1005, 1299, 1419, 2931, 3141
Bst2BI CACGAG 1 cut(s) 2466
Bst2UI CCWGG 5 cut(s) 581, 836, 2161, 2646, 3053
Bst4CI ACNGT 7 cut(s) 730, 1525, 1696, 2005, 2270, 2563, 2986
Bst6I CTCTTC 2 cut(s) 1628, 3225
BstAPI GCANNNNNTGC 2 cut(s) 37, 2477
BstAUI TGTACA 1 cut(s) 2853
BstDSI CCRYGG 5 cut(s) 90, 868, 1299, 2931, 3141
BstF5I GGATG 9 cut(s) 345, 472, 844, 1177, 1363, 1550, 1816, 2308, 2686
BstFNI CGCG 2 cut(s) 117, 3065
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 3 cut(s) 306, 356, 3129
BstMAI GTCTC 9 cut(s) 290, 337, 991, 1409, 1585, 2204, 2423, 2469, 2534
BstNI CCWGG 5 cut(s) 581, 836, 2161, 2646, 3053
BstNSI RCATGY 2 cut(s) 810, 3109
BstSCI CCNGG 6 cut(s) 579, 834, 2048, 2159, 2644, 3051
BstSFI CTRYAG 3 cut(s) 585, 1748, 2363
BstSLI GKGCMC 6 cut(s) 487, 1540, 2003, 2050, 2239, 2877
BstUI CGCG 2 cut(s) 117, 3065
BstV2I GAAGAC 2 cut(s) 2892, 3054
BstX2I RGATCY 5 cut(s) 259, 751, 1163, 1423, 1529
BstYI RGATCY 5 cut(s) 259, 751, 1163, 1423, 1529
Bsu15I ATCGAT 3 cut(s) 252, 776, 2576
Bsu36I CCTNAGG 1 cut(s) 2298
BsuRI GGCC 8 cut(s) 485, 687, 717, 1346, 2048, 2159, 2237, 2835
BsuTUI ATCGAT 3 cut(s) 252, 776, 2576
BtgI CCRYGG 5 cut(s) 90, 868, 1299, 2931, 3141
BtgZI GCGATG 1 cut(s) 12
BtrI CACGTC 1 cut(s) 3205
BtsCI GGATG 9 cut(s) 345, 472, 844, 1177, 1363, 1550, 1816, 2308, 2686
BtsI GCAGTG 3 cut(s) 66, 1745, 2360
BtsIMutI CAGTG 6 cut(s) 66, 594, 726, 1521, 1745, 2360
CaiI CAGNNNCTG 2 cut(s) 1700, 1772
CfoI GCGC 3 cut(s) 306, 356, 3129
Cfr10I RCCGGY 4 cut(s) 270, 306, 383, 2831
ClaI ATCGAT 3 cut(s) 252, 776, 2576
Csp6I GTAC 5 cut(s) 680, 1873, 2504, 2854, 3067
CviQI GTAC 5 cut(s) 680, 1873, 2504, 2854, 3067
DraI TTTAAA 1 cut(s) 3024
Eam1104I CTCTTC 2 cut(s) 1628, 3225
EarI CTCTTC 2 cut(s) 1628, 3225
EciI GGCGGA 1 cut(s) 81
Ecl136II GAGCTC 1 cut(s) 240
Eco130I CCWWGG 6 cut(s) 868, 1005, 1299, 1419, 2931, 3141
Eco147I AGGCCT 2 cut(s) 687, 1346
Eco24I GRGCYC 4 cut(s) 242, 487, 2050, 2239
Eco31I GGTCTC 2 cut(s) 290, 1409
Eco32I GATATC 2 cut(s) 250, 3247
Eco47I GGWCC 3 cut(s) 1972, 2704, 3138
Eco53kI GAGCTC 1 cut(s) 240
Eco57I CTGAAG 3 cut(s) 1395, 2109, 2264
Eco81I CCTNAGG 1 cut(s) 2298
Eco88I CYCGRG 1 cut(s) 334
EcoICRI GAGCTC 1 cut(s) 240
EcoO109I RGGNCCY 2 cut(s) 484, 1972
EcoRI GAATTC 1 cut(s) 623
EcoRII CCWGG 5 cut(s) 579, 834, 2159, 2644, 3051
EcoRV GATATC 2 cut(s) 250, 3247
EcoT14I CCWWGG 6 cut(s) 868, 1005, 1299, 1419, 2931, 3141
EcoT38I GRGCYC 4 cut(s) 242, 487, 2050, 2239
ErhI CCWWGG 6 cut(s) 868, 1005, 1299, 1419, 2931, 3141
Esp3I CGTCTC 2 cut(s) 337, 2204
FaqI GGGAC 5 cut(s) 446, 1985, 2335, 2930, 3005
FauI CCCGC 1 cut(s) 3258
FauNDI CATATG 2 cut(s) 967, 2700
FbaI TGATCA 1 cut(s) 1858
FblI GTMKAC 2 cut(s) 2068, 2760
FokI GGATG 9 cut(s) 352, 479, 851, 1184, 1350, 1557, 1823, 2315, 2693
FriOI GRGCYC 4 cut(s) 242, 487, 2050, 2239
FspBI CTAG 5 cut(s) 45, 1121, 1281, 1541, 1907
GlaI GCGC 3 cut(s) 305, 355, 3128
GsaI CCCAGC 1 cut(s) 2236
GsuI CTGGAG 3 cut(s) 1475, 1523, 2378
HaeII RGCGCY 1 cut(s) 357
HaeIII GGCC 8 cut(s) 485, 687, 717, 1346, 2048, 2159, 2237, 2835
HapII CCGG 7 cut(s) 18, 271, 307, 384, 2050, 2507, 2832
HhaI GCGC 3 cut(s) 306, 356, 3129
Hin6I GCGC 3 cut(s) 304, 354, 3127
HinP1I GCGC 3 cut(s) 304, 354, 3127
HincII GTYRAC 4 cut(s) 1605, 2283, 2491, 2761
HindII GTYRAC 4 cut(s) 1605, 2283, 2491, 2761
HindIII AAGCTT 1 cut(s) 1232
HpaI GTTAAC 3 cut(s) 1605, 2283, 2491
HpaII CCGG 7 cut(s) 18, 271, 307, 384, 2050, 2507, 2832
HphI GGTGA 8 cut(s) 758, 850, 1006, 1064, 1729, 1934, 2159, 2765
HpyCH4III ACNGT 7 cut(s) 730, 1525, 1696, 2005, 2270, 2563, 2986
HpyCH4IV ACGT 2 cut(s) 2213, 3204
HpySE526I ACGT 2 cut(s) 2213, 3204
HspAI GCGC 3 cut(s) 304, 354, 3127
KflI GGGWCCC 1 cut(s) 1972
KpnI GGTACC 1 cut(s) 2507
KroI GCCGGC 2 cut(s) 270, 306
KroNI GCCGGC 2 cut(s) 272, 308
Ksp22I TGATCA 1 cut(s) 1858
KspAI GTTAAC 3 cut(s) 1605, 2283, 2491
LmnI GCTCC 9 cut(s) 119, 237, 1481, 1494, 1961, 2632, 2848, 3036, 3124
MaeI CTAG 5 cut(s) 45, 1121, 1281, 1541, 1907
MaeII ACGT 2 cut(s) 2213, 3204
MaeIII GTNAC 6 cut(s) 397, 707, 1052, 1067, 1198, 1637
MbiI CCGCTC 1 cut(s) 2635
MfeI CAATTG 2 cut(s) 618, 2805
MflI RGATCY 5 cut(s) 259, 751, 1163, 1423, 1529
MhlI GDGCHC 7 cut(s) 242, 487, 1540, 2003, 2050, 2239, 2877
MluI ACGCGT 1 cut(s) 3063
MlyI GAGTC 1 cut(s) 1904
MmeI TCCRAC 4 cut(s) 345, 451, 1306, 1507
MroNI GCCGGC 2 cut(s) 270, 306
MroXI GAANNNNTTC 1 cut(s) 1404
MslI CAYNNNNRTG 6 cut(s) 411, 1193, 1514, 1543, 1928, 1996
MspA1I CMGCKG 1 cut(s) 2087
MspI CCGG 7 cut(s) 18, 271, 307, 384, 2050, 2507, 2832
MspR9I CCNGG 6 cut(s) 581, 836, 2050, 2161, 2646, 3053
MunI CAATTG 2 cut(s) 618, 2805
Mva1269I GAATGC 3 cut(s) 919, 1846, 2192
MvaI CCWGG 5 cut(s) 581, 836, 2161, 2646, 3053
MvnI CGCG 2 cut(s) 117, 3065
NaeI GCCGGC 2 cut(s) 272, 308
NciI CCSGG 1 cut(s) 2050
NcoI CCATGG 4 cut(s) 868, 1299, 2931, 3141
NdeI CATATG 2 cut(s) 967, 2700
NgoMIV GCCGGC 2 cut(s) 270, 306
NmeAIII GCCGAG 1 cut(s) 924
NmuCI GTSAC 4 cut(s) 397, 707, 1052, 1067
NspI RCATGY 2 cut(s) 810, 3109
OliI CACNNNNGTG 2 cut(s) 1514, 1996
PceI AGGCCT 2 cut(s) 687, 1346
PcsI WCGNNNNNNNCGW 2 cut(s) 98, 3164
PctI GAATGC 3 cut(s) 919, 1846, 2192
PdiI GCCGGC 2 cut(s) 272, 308
PdmI GAANNNNTTC 1 cut(s) 1404
PfeI GAWTC 9 cut(s) 95, 137, 209, 478, 1391, 1400, 1753, 2665, 2793
PfoI TCCNGGA 1 cut(s) 834
PleI GAGTC 1 cut(s) 1903
PpsI GAGTC 1 cut(s) 1903
PpuMI RGGWCCY 1 cut(s) 1972
PsiI TTATAA 2 cut(s) 668, 3005
Psp124BI GAGCTC 1 cut(s) 242
Psp5II RGGWCCY 1 cut(s) 1972
Psp6I CCWGG 5 cut(s) 579, 834, 2159, 2644, 3051
PspFI CCCAGC 1 cut(s) 2232
PspGI CCWGG 5 cut(s) 579, 834, 2159, 2644, 3051
PspOMI GGGCCC 3 cut(s) 483, 2046, 2235
PspPPI RGGWCCY 1 cut(s) 1972
PstI CTGCAG 2 cut(s) 1752, 2367
PstNI CAGNNNCTG 2 cut(s) 1700, 1772
PsuI RGATCY 5 cut(s) 259, 751, 1163, 1423, 1529
PvuII CAGCTG 1 cut(s) 2087
RsaI GTAC 5 cut(s) 681, 1874, 2505, 2855, 3068
RsaNI GTAC 5 cut(s) 680, 1873, 2504, 2854, 3067
RseI CAYNNNNRTG 6 cut(s) 411, 1193, 1514, 1543, 1928, 1996
SacI GAGCTC 1 cut(s) 242
SalI GTCGAC 1 cut(s) 2759
SchI GAGTC 1 cut(s) 1904
ScrFI CCNGG 6 cut(s) 581, 836, 2050, 2161, 2646, 3053
SduI GDGCHC 7 cut(s) 242, 487, 1540, 2003, 2050, 2239, 2877
SfcI CTRYAG 3 cut(s) 585, 1748, 2363
SinI GGWCC 3 cut(s) 1972, 2704, 3138
SmiMI CAYNNNNRTG 6 cut(s) 411, 1193, 1514, 1543, 1928, 1996
SmlI CTYRAG 5 cut(s) 827, 1040, 1235, 2072, 3035
SmoI CTYRAG 5 cut(s) 827, 1040, 1235, 2072, 3035
SseBI AGGCCT 2 cut(s) 687, 1346
SsiI CCGC 8 cut(s) 66, 117, 281, 755, 2130, 2591, 2635, 3251
SspMI CTAG 5 cut(s) 45, 1121, 1281, 1541, 1907
SstI GAGCTC 1 cut(s) 242
StuI AGGCCT 2 cut(s) 687, 1346
StyD4I CCNGG 6 cut(s) 579, 834, 2048, 2159, 2644, 3051
StyI CCWWGG 6 cut(s) 868, 1005, 1299, 1419, 2931, 3141
TaaI ACNGT 7 cut(s) 730, 1525, 1696, 2005, 2270, 2563, 2986
TaiI ACGT 2 cut(s) 2216, 3207
TaqII GACCGA 1 cut(s) 893
TatI WGTACW 2 cut(s) 1872, 2853
TauI GCSGC 2 cut(s) 2132, 2638
TfiI GAWTC 9 cut(s) 95, 137, 209, 478, 1391, 1400, 1753, 2665, 2793
TscAI CASTG 6 cut(s) 66, 601, 733, 1528, 1752, 2367
TseFI GTSAC 4 cut(s) 397, 707, 1052, 1067
Tsp45I GTSAC 4 cut(s) 397, 707, 1052, 1067
TspGWI ACGGA 2 cut(s) 107, 2883
TspRI CASTG 6 cut(s) 66, 601, 733, 1528, 1752, 2367
VneI GTGCAC 1 cut(s) 1999
VpaK11BI GGWCC 3 cut(s) 1972, 2704, 3138
XapI RAATTY 6 cut(s) 623, 1184, 1467, 1561, 1804, 2777
XceI RCATGY 2 cut(s) 810, 3109
XcmI CCANNNNNNNNNTGG 3 cut(s) 2167, 2451, 3148
XmiI GTMKAC 2 cut(s) 2068, 2760
XmnI GAANNNNTTC 1 cut(s) 1404
XspI CTAG 5 cut(s) 45, 1121, 1281, 1541, 1907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.