MD12G1177300.v1.1

Ubiquitin-activating enzyme E1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002217
Physical Location & Seq
Reverse (-)
25765037 .. 25771017
5981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1177300.v1.1.491

Sequence Viewer

Length: 3057 bp
ATGGCATTGGGCAACGGGAGCTCGAACGATATCGATGAAGATCTCCACAGTCGGCAGCTGGCGGTTTATGGAAGGGAGACCATGCGCCGGCTCTTTGCCTCCAACATTCTCGTCTCTGGAATGCAGGGCCTCGGCGCTGAGATTGCTAAAAACCTTGTTCTTGCCGGTGTCAAGTCTGTGACCTTACATGATGATGGAGTTGTTGAGTTGTGGGACTTGTCAAGCAACTTCTTCTTTTCTGAGGATGATGTTGGAAAGAATCGGGCCCTTGCCTGTGTCCAAAAATTACAAGAACTAAACAACGCCGTTCTCATTTCGACCATAACAACTGAATTGACAAAAGAGAAACTCTCTGATTTCCAGGCTGTAGTTTTCACTGATATTAGCTTGGAGAAAGCAATTGAATTCGATGACTACTGCCATAATCATAAGCCTCCCATATCTTTTATAAAATCAGAAGTACGAGGCCTTTTTGGCAGTGTGTTTTGTGACTTTGGCCCTGAGTTCACTGTTCTTGATGTTGATGGTGAGGATCCACATACAGGCATAATTGCATCGATTAGTAATGACAACCCCGCTCTCATAGCATGCGTTGATGATGAGAGGCTTGAGTTCCAGGATGGGGATTTAGTTGTGTTCACCGAAGTCCATGGAATGACTGAGTTGAATGATGGAAAGCCGAGGAAGGTTAAGAATGCAAGACCCTACTCATTCACAATCGAGGAGGACACTACCAATTATGCTGCGTATGAGAAAGGGGGTATAGTCACACAGGTGAAGCAACCCAAGGTGTTGAACTTTAAGCCTTTGCGAGAAGCACTCAAGGATCATGGCGACTTCCTTCTAATTGACTTCTCCAAGTTTGATCGCCCACCTCTCCTACACTTGGCCTTTCAGGCACTAGATAAGTTCATCTCAGAGTTGGGGCGTTTCCCTGTTGCTGGATCTGAGGATGATGCTACAAAATTCATATCTTTGGTAACTAACATCAATGATAGCTCAGAAGATGGGAAGCTTGAGGAGATTGACCACAAGATTCTTCGTCATTTTGCATTTGGTGCTAGAGCTGTGCTAAATCCCATGGCTGCTATGTTTGGTGGTATTGTTGGACAAGAAGTTGTGAAGGCCTGTTCTGCTAAGTTCCATCCTCTTTTCCAGTTTTTCTACTTCGATTCAGTTGAATCCCTTCCGTCAGGGACCTTGGATCCTAATGATTTGAAGCCTTTGAACAGCCGATATGATGCACAAATTTCAGTATTCGGAGCCAAGCTCCAGAAAAAGCTGGAGGATGCAAAAGTGTTCACTGTCGGATCTGGTGCACTAGGATGTGAGTTTTTGAAGAATTTAGCATTGATGGGTGTGTCTTGTGGTAAAGAAGGGAAGTTAACAATTACAGATGATGATGTTATTGAGAAGAGTAACCTTAGCAGGCAATTTCTCTTCCGAGATTGGAACATTGGGCAGGCTAAATCAACAGTAGCTGCCTCTGCTGCTACGTTGATAAATGGTCATCTGAACGTTGAAGCACTCCAGAATCGTGCAAGCCCGGATACTGAAAATGTGTTTGATGATACTTTTTGGGAGAATTTGGATGTTGTTATCAATGCTCTGGACAATGTGAATGCAAGGCTTTACATTGATCAGAGATGCTTGTATTTCCAGAAGCCCCTTTTGGAATCAGGAACACTAGGTGCCAAGTGCAACACGCAGATGGTCATTCCTCACCTTACTGAAAATTATGGAGCATCGCGGGACCCACCTGAAAAGCAAGCACCCATGTGCACAGTCCATTCATTCCCTCACAACATTGACCACTGCTTGACCTGGGCCCGATCTGAGTTTGAGGGTCTACTTGAGAAGGTGCCAGCTGAAGTAAATGCCTACCTGACCAATCCTCAAGAATACACTGCTGCAATGAAGAATGCTGGTGATGCTCAGGCCAGGAACAACTTGGAAAGTGTCATTGAATGCCTTGACAAGGAGAGATGTGAGACGTTTCAAGATTGCATAAACTGGGCCCGTCTAAAATTTGAGGACTACTTTGTCAACCGTGTGAAGCAGTTAACATATACTTTTCCTGAGGATGCTACAACCAGTAGCGGGACACCATTTTGGTCTGCCCCCAAGCGTTTTCCTCGCCCACTGCAGTTCTCAGTTGATGATCTCAGCCACCTTCAGTTTTTAATGGCAGCATCCATACTAAGAGCAGAGACATTCAACATTCCAATTCCTGATTGGGTAAAGTCTCGTACAAAGTTTGCTGATGCTGTTAACAAGGTGATAGTACCAGATTTTCAGCCTAAGAAAGATGTGAAGATTGAGACTGATGAAAAATCTACAAGCGTCTTACCAGCATCGATTGATGATGCTGCAGTTATCAATGAGTTAGTTGTGAAGTTAGAAAAATGCAAGGAGCGGCTGCCACCAGGCTTCAAGATGAACCCGATTCAGTTTGAGAAGGACGATGACACAAACTATCATATGGACCTGATAGCTGGATTTGCAAACATGAGGGCGAGGAACTATGGCATTGGTGAAGTCGACAAGCTGAAAGCCAAATTCATTGCAGGAAGAATCATTCCTGCGATTGCAACAGCTACTGCTCTGGCAACTGGCCTTGTCTGCTTGGAGCTGTACAAGGTTCTGGATGGAGGGCACACGGTTGAGGACTACCGAAACACCTTTGCCAATCTCTCCCTTCCTCTGTTCTCCATGGCTGAACCAGTTCCTCCTAAAGTCATCAAGCACCAAGATATGAAGTGGACAGTATGGGATAGGTGGATTATAAGGGACAACCCAACTTTAAAGCAGCTCCTCAAGTGGCTCGAAGACCAGGGCCTAAGCGCGTACAGCATCTCCTATGGAAGTTGCCTGCTCTTTAACAGCATGTTCCCGAAGCACAAGGAGCGCATGGACAGGACCATGGTGGACTTGGCTACGAGTATTGCGAAGGCAGAACTACCTCCAAATAGGAAGCACTTTGATGTGGTGGTGGCTTGCGAAGATGAAGAAGAGAACGACATTGATATCCCGCAAATCTCCATTTACTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko05012 map04120 map05012
Pfam Domains
Protein Families
E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1019

Amino Acids

113.97

Weight (kDa)

5.08

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 16 - 380 8.2e-25 ThiF family
E1_FCCH PF16190 190 - 260 4e-28 Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB PF16191 261 - 331 6.1e-24 Ubiquitin-activating enzyme E1 four-helix bundle
ThiF PF00899 413 - 905 6.7e-60 ThiF family
UBA_E1_SCCH PF10585 600 - 853 4.2e-86 Ubiquitin-activating enzyme, SCCH domain
E1_UFD PF09358 924 - 1013 1.8e-23 Ubiquitin fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 449, 2786
AasI GACNNNNNNGTC 1 cut(s) 2042
AccB1I GGYRCC 2 cut(s) 1691, 1861
AccBSI CCGCTC 2 cut(s) 578, 2416
AccI GTMKAC 2 cut(s) 1849, 2541
AccII CGCG 2 cut(s) 1750, 2846
AciI CCGC 6 cut(s) 62, 576, 1750, 2100, 2416, 3032
AclI AACGTT 1 cut(s) 1518
AclWI GGATC 7 cut(s) 527, 540, 834, 952, 1199, 1212, 1318
AcsI RAATTY 7 cut(s) 404, 965, 1248, 1342, 1585, 2027, 2558
AcuI CTGAAG 2 cut(s) 1890, 2159
AdeI CACNNNGTG 1 cut(s) 1691
AfaI GTAC 5 cut(s) 462, 2251, 2286, 2636, 2849
AfiI CCNNNNNNNGG 7 cut(s) 87, 542, 622, 886, 941, 1978, 2100
AjnI CCWGG 6 cut(s) 360, 615, 1823, 1940, 2425, 2832
AjuI GAANNNNNNNTTGG 2 cut(s) 1655, 1687
AleI CACNNNNGTG 2 cut(s) 773, 1777
Alw21I GWGCWC 3 cut(s) 23, 1321, 1784
Alw26I GTCTC 6 cut(s) 71, 118, 1985, 2204, 2250, 2315
Alw44I GTGCAC 2 cut(s) 1317, 1780
AlwI GGATC 7 cut(s) 527, 540, 834, 952, 1199, 1212, 1318
AlwNI CAGNNNCTG 2 cut(s) 1481, 2600
ApaI GGGCCC 3 cut(s) 268, 1831, 2020
ApaLI GTGCAC 2 cut(s) 1317, 1780
ApoI RAATTY 7 cut(s) 404, 965, 1248, 1342, 1585, 2027, 2558
Asp700I GAANNNNTTC 2 cut(s) 1185, 2724
AspLEI GCGC 4 cut(s) 87, 137, 2846, 2910
AsuC2I CCSGG 1 cut(s) 1547
AsuHPI GGTGA 7 cut(s) 539, 631, 787, 1715, 1940, 2290, 2546
AvaII GGWCC 4 cut(s) 1197, 1753, 2485, 2919
AxyI CCTNAGG 1 cut(s) 2079
BaeGI GKGCMC 6 cut(s) 268, 1321, 1784, 1831, 2020, 2658
BamHI GGATCC 2 cut(s) 532, 1204
BanI GGYRCC 2 cut(s) 1691, 1861
BanII GRGCYC 4 cut(s) 23, 268, 1831, 2020
BbsI GAAGAC 1 cut(s) 2835
Bbv12I GWGCWC 3 cut(s) 23, 1321, 1784
BccI CCATC 9 cut(s) 188, 518, 614, 665, 1001, 1152, 1348, 1705, 2642
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 6 cut(s) 362, 617, 1825, 1942, 2427, 2834
BciVI GTATCC 1 cut(s) 1543
BclI TGATCA 1 cut(s) 1639
BcnI CCSGG 1 cut(s) 1547
BcoDI GTCTC 6 cut(s) 71, 118, 1985, 2204, 2250, 2315
BfaI CTAG 4 cut(s) 902, 1062, 1322, 1688
BfmI CTRYAG 3 cut(s) 366, 2144, 2370
BfoI RGCGCY 1 cut(s) 138
BfuI GTATCC 1 cut(s) 1543
BglI GCCNNNNNGGC 2 cut(s) 474, 896
BglII AGATCT 1 cut(s) 40
Bme1390I CCNGG 7 cut(s) 362, 617, 1547, 1825, 1942, 2427, 2834
Bme18I GGWCC 4 cut(s) 1197, 1753, 2485, 2919
BmrFI CCNGG 7 cut(s) 362, 617, 1547, 1825, 1942, 2427, 2834
BmrI ACTGGG 1 cut(s) 2023
BmuI ACTGGG 1 cut(s) 2023
BpiI GAAGAC 1 cut(s) 2835
BplI GAGNNNNNCTC 6 cut(s) 335, 367, 804, 836, 1254, 1286
BpmI CTGGAG 3 cut(s) 1256, 1304, 1514
Bpu10I CCTNAGC 3 cut(s) 1424, 1935, 2840
BpuEI CTTGAG 6 cut(s) 629, 806, 1037, 1874, 1881, 2801
BpuMI CCSGG 1 cut(s) 1547
Bsa29I ATCGAT 3 cut(s) 33, 557, 2357
BsaBI GATNNNNATC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 71
BsaXI ACNNNNNCTCC 2 cut(s) 2840, 2870
Bsc4I CCNNNNNNNGG 7 cut(s) 87, 542, 622, 886, 941, 1978, 2100
Bse118I RCCGGY 2 cut(s) 87, 164
Bse1I ACTGG 5 cut(s) 1156, 2018, 2094, 2617, 2723
Bse21I CCTNAGG 1 cut(s) 2079
Bse3DI GCAATG 2 cut(s) 1920, 2562
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 6 cut(s) 362, 617, 1825, 1942, 2427, 2834
BseCI ATCGAT 3 cut(s) 33, 557, 2357
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 7 cut(s) 87, 542, 622, 886, 941, 1978, 2100
BseMI GCAATG 2 cut(s) 1920, 2562
BseNI ACTGG 5 cut(s) 1156, 2018, 2094, 2617, 2723
BseRI GAGGAG 3 cut(s) 737, 1034, 2804
BseSI GKGCMC 6 cut(s) 268, 1321, 1784, 1831, 2020, 2658
Bsh1236I CGCG 2 cut(s) 1750, 2846
BshNI GGYRCC 2 cut(s) 1691, 1861
BshVI ATCGAT 3 cut(s) 33, 557, 2357
BsiHKAI GWGCWC 3 cut(s) 23, 1321, 1784
BsiSI CCGG 3 cut(s) 88, 165, 1547
BslFI GGGAC 5 cut(s) 227, 1210, 1766, 2116, 2804
BslI CCNNNNNNNGG 7 cut(s) 87, 542, 622, 886, 941, 1978, 2100
BsmAI GTCTC 6 cut(s) 71, 118, 1985, 2204, 2250, 2315
BsmBI CGTCTC 2 cut(s) 118, 1985
BsmFI GGGAC 5 cut(s) 227, 1210, 1766, 2116, 2804
BsmI GAATGC 5 cut(s) 126, 700, 1627, 1927, 1973
Bso31I GGTCTC 1 cut(s) 71
Bsp120I GGGCCC 3 cut(s) 264, 1827, 2016
Bsp1286I GDGCHC 7 cut(s) 23, 268, 1321, 1784, 1831, 2020, 2658
Bsp1407I TGTACA 1 cut(s) 2634
Bsp19I CCATGG 4 cut(s) 649, 1080, 2712, 2922
BspACI CCGC 6 cut(s) 62, 576, 1750, 2100, 2416, 3032
BspDI ATCGAT 3 cut(s) 33, 557, 2357
BspFNI CGCG 2 cut(s) 1750, 2846
BspMAI CTGCAG 2 cut(s) 2148, 2374
BspPI GGATC 7 cut(s) 527, 540, 834, 952, 1199, 1212, 1318
BspT107I GGYRCC 2 cut(s) 1691, 1861
BspTNI GGTCTC 1 cut(s) 71
BsrBI CCGCTC 2 cut(s) 578, 2416
BsrDI GCAATG 2 cut(s) 1920, 2562
BsrFI RCCGGY 2 cut(s) 87, 164
BsrGI TGTACA 1 cut(s) 2634
BsrI ACTGG 5 cut(s) 1156, 2018, 2094, 2617, 2723
BssAI RCCGGY 2 cut(s) 87, 164
BssT1I CCWWGG 6 cut(s) 649, 786, 1080, 1200, 2712, 2922
Bst2UI CCWGG 6 cut(s) 362, 617, 1825, 1942, 2427, 2834
Bst4CI ACNGT 8 cut(s) 50, 511, 1306, 1477, 1786, 2051, 2662, 2767
Bst6I CTCTTC 3 cut(s) 1409, 1445, 3006
BstAPI GCANNNNNTGC 1 cut(s) 1058
BstAUI TGTACA 1 cut(s) 2634
BstDSI CCRYGG 4 cut(s) 649, 1080, 2712, 2922
BstENI CCTNNNNNAGG 1 cut(s) 1976
BstFNI CGCG 2 cut(s) 1750, 2846
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 4 cut(s) 87, 137, 2846, 2910
BstMAI GTCTC 6 cut(s) 71, 118, 1985, 2204, 2250, 2315
BstNI CCWGG 6 cut(s) 362, 617, 1825, 1942, 2427, 2834
BstNSI RCATGY 2 cut(s) 591, 2890
BstSCI CCNGG 7 cut(s) 360, 615, 1545, 1823, 1940, 2425, 2832
BstSFI CTRYAG 3 cut(s) 366, 2144, 2370
BstSLI GKGCMC 6 cut(s) 268, 1321, 1784, 1831, 2020, 2658
BstUI CGCG 2 cut(s) 1750, 2846
BstV2I GAAGAC 1 cut(s) 2835
BstX2I RGATCY 5 cut(s) 40, 532, 944, 1204, 1310
BstYI RGATCY 5 cut(s) 40, 532, 944, 1204, 1310
Bsu15I ATCGAT 3 cut(s) 33, 557, 2357
Bsu36I CCTNAGG 1 cut(s) 2079
BsuI GTATCC 1 cut(s) 1543
BsuTUI ATCGAT 3 cut(s) 33, 557, 2357
BtgI CCRYGG 4 cut(s) 649, 1080, 2712, 2922
BtgZI GCGATG 1 cut(s) 1731
BtsI GCAGTG 4 cut(s) 484, 1813, 1905, 2141
BtsIMutI CAGTG 7 cut(s) 375, 484, 507, 1302, 1813, 1905, 2141
CaiI CAGNNNCTG 2 cut(s) 1481, 2600
CfoI GCGC 4 cut(s) 87, 137, 2846, 2910
Cfr10I RCCGGY 2 cut(s) 87, 164
ClaI ATCGAT 3 cut(s) 33, 557, 2357
CseI GACGC 1 cut(s) 2332
Csp6I GTAC 5 cut(s) 461, 2250, 2285, 2635, 2848
CviQI GTAC 5 cut(s) 461, 2250, 2285, 2635, 2848
DraI TTTAAA 1 cut(s) 2805
DraIII CACNNNGTG 1 cut(s) 1691
DrdI GACNNNNNNGTC 1 cut(s) 2042
DseDI GACNNNNNNGTC 1 cut(s) 2042
Eam1104I CTCTTC 3 cut(s) 1409, 1445, 3006
EarI CTCTTC 3 cut(s) 1409, 1445, 3006
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 6 cut(s) 649, 786, 1080, 1200, 2712, 2922
Eco147I AGGCCT 2 cut(s) 468, 1127
Eco24I GRGCYC 4 cut(s) 23, 268, 1831, 2020
Eco31I GGTCTC 1 cut(s) 71
Eco32I GATATC 2 cut(s) 31, 3028
Eco47I GGWCC 4 cut(s) 1197, 1753, 2485, 2919
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 2 cut(s) 1890, 2159
Eco81I CCTNAGG 1 cut(s) 2079
EcoICRI GAGCTC 1 cut(s) 21
EcoNI CCTNNNNNAGG 1 cut(s) 1976
EcoO109I RGGNCCY 5 cut(s) 127, 265, 1197, 1753, 2836
EcoRI GAATTC 1 cut(s) 404
EcoRII CCWGG 6 cut(s) 360, 615, 1823, 1940, 2425, 2832
EcoRV GATATC 2 cut(s) 31, 3028
EcoT14I CCWWGG 6 cut(s) 649, 786, 1080, 1200, 2712, 2922
EcoT38I GRGCYC 4 cut(s) 23, 268, 1831, 2020
ErhI CCWWGG 6 cut(s) 649, 786, 1080, 1200, 2712, 2922
Esp3I CGTCTC 2 cut(s) 118, 1985
FaqI GGGAC 5 cut(s) 227, 1210, 1766, 2116, 2804
FauI CCCGC 4 cut(s) 583, 1743, 2093, 3039
FauNDI CATATG 1 cut(s) 2481
FbaI TGATCA 1 cut(s) 1639
FblI GTMKAC 2 cut(s) 1849, 2541
FriOI GRGCYC 4 cut(s) 23, 268, 1831, 2020
FspBI CTAG 4 cut(s) 902, 1062, 1322, 1688
GlaI GCGC 4 cut(s) 86, 136, 2845, 2909
GsuI CTGGAG 3 cut(s) 1256, 1304, 1514
HaeII RGCGCY 1 cut(s) 138
HapII CCGG 3 cut(s) 88, 165, 1547
HgaI GACGC 1 cut(s) 2332
HhaI GCGC 4 cut(s) 87, 137, 2846, 2910
Hin6I GCGC 4 cut(s) 85, 135, 2844, 2908
HinP1I GCGC 4 cut(s) 85, 135, 2844, 2908
HincII GTYRAC 5 cut(s) 1386, 2047, 2064, 2272, 2542
HindII GTYRAC 5 cut(s) 1386, 2047, 2064, 2272, 2542
HindIII AAGCTT 1 cut(s) 1013
HinfI GANTC 8 cut(s) 259, 1036, 1172, 1181, 1534, 1676, 2446, 2574
HpaI GTTAAC 3 cut(s) 1386, 2064, 2272
HpaII CCGG 3 cut(s) 88, 165, 1547
HphI GGTGA 7 cut(s) 539, 631, 787, 1715, 1940, 2290, 2546
HpyCH4III ACNGT 8 cut(s) 50, 511, 1306, 1477, 1786, 2051, 2662, 2767
HpyCH4IV ACGT 3 cut(s) 1496, 1518, 1994
HpySE526I ACGT 3 cut(s) 1496, 1518, 1994
HspAI GCGC 4 cut(s) 85, 135, 2844, 2908
KflI GGGWCCC 1 cut(s) 1753
KroI GCCGGC 1 cut(s) 87
KroNI GCCGGC 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 1639
KspAI GTTAAC 3 cut(s) 1386, 2064, 2272
LmnI GCTCC 8 cut(s) 18, 1262, 1275, 1742, 2413, 2629, 2817, 2905
MaeI CTAG 4 cut(s) 902, 1062, 1322, 1688
MaeII ACGT 3 cut(s) 1496, 1518, 1994
MaeIII GTNAC 5 cut(s) 178, 488, 766, 979, 1418
MbiI CCGCTC 2 cut(s) 578, 2416
MfeI CAATTG 1 cut(s) 399
MflI RGATCY 5 cut(s) 40, 532, 944, 1204, 1310
MhlI GDGCHC 7 cut(s) 23, 268, 1321, 1784, 1831, 2020, 2658
MmeI TCCRAC 4 cut(s) 126, 232, 1087, 1288
MroNI GCCGGC 1 cut(s) 87
MroXI GAANNNNTTC 2 cut(s) 1185, 2724
MseI TTAA 8 cut(s) 690, 801, 1385, 2063, 2183, 2271, 2804, 2880
MslI CAYNNNNRTG 6 cut(s) 192, 773, 1324, 1709, 1777, 2982
MspA1I CMGCKG 2 cut(s) 58, 1868
MspI CCGG 3 cut(s) 88, 165, 1547
MspR9I CCNGG 7 cut(s) 362, 617, 1547, 1825, 1942, 2427, 2834
MunI CAATTG 1 cut(s) 399
Mva1269I GAATGC 5 cut(s) 126, 700, 1627, 1927, 1973
MvaI CCWGG 6 cut(s) 362, 617, 1825, 1942, 2427, 2834
MvnI CGCG 2 cut(s) 1750, 2846
NaeI GCCGGC 1 cut(s) 89
NciI CCSGG 1 cut(s) 1547
NcoI CCATGG 4 cut(s) 649, 1080, 2712, 2922
NdeI CATATG 1 cut(s) 2481
NgoMIV GCCGGC 1 cut(s) 87
NmeAIII GCCGAG 2 cut(s) 111, 705
NmuCI GTSAC 3 cut(s) 178, 488, 766
NspI RCATGY 2 cut(s) 591, 2890
OliI CACNNNNGTG 2 cut(s) 773, 1777
PaeI GCATGC 1 cut(s) 591
PceI AGGCCT 2 cut(s) 468, 1127
PcsI WCGNNNNNNNCGW 1 cut(s) 2945
PctI GAATGC 5 cut(s) 126, 700, 1627, 1927, 1973
PdiI GCCGGC 1 cut(s) 89
PdmI GAANNNNTTC 2 cut(s) 1185, 2724
PfeI GAWTC 8 cut(s) 259, 1036, 1172, 1181, 1534, 1676, 2446, 2574
PfoI TCCNGGA 1 cut(s) 615
PpuMI RGGWCCY 2 cut(s) 1197, 1753
PsiI TTATAA 2 cut(s) 449, 2786
Psp124BI GAGCTC 1 cut(s) 23
Psp1406I AACGTT 1 cut(s) 1518
Psp5II RGGWCCY 2 cut(s) 1197, 1753
Psp6I CCWGG 6 cut(s) 360, 615, 1823, 1940, 2425, 2832
PspGI CCWGG 6 cut(s) 360, 615, 1823, 1940, 2425, 2832
PspOMI GGGCCC 3 cut(s) 264, 1827, 2016
PspPPI RGGWCCY 2 cut(s) 1197, 1753
PstI CTGCAG 2 cut(s) 2148, 2374
PstNI CAGNNNCTG 2 cut(s) 1481, 2600
PsuI RGATCY 5 cut(s) 40, 532, 944, 1204, 1310
PvuII CAGCTG 2 cut(s) 58, 1868
RsaI GTAC 5 cut(s) 462, 2251, 2286, 2636, 2849
RsaNI GTAC 5 cut(s) 461, 2250, 2285, 2635, 2848
RseI CAYNNNNRTG 6 cut(s) 192, 773, 1324, 1709, 1777, 2982
SacI GAGCTC 1 cut(s) 23
SalI GTCGAC 1 cut(s) 2540
SaqAI TTAA 8 cut(s) 690, 801, 1385, 2063, 2183, 2271, 2804, 2880
ScrFI CCNGG 7 cut(s) 362, 617, 1547, 1825, 1942, 2427, 2834
SduI GDGCHC 7 cut(s) 23, 268, 1321, 1784, 1831, 2020, 2658
SfcI CTRYAG 3 cut(s) 366, 2144, 2370
SinI GGWCC 4 cut(s) 1197, 1753, 2485, 2919
SmiMI CAYNNNNRTG 6 cut(s) 192, 773, 1324, 1709, 1777, 2982
SmlI CTYRAG 6 cut(s) 608, 821, 1016, 1853, 1896, 2816
SmoI CTYRAG 6 cut(s) 608, 821, 1016, 1853, 1896, 2816
SphI GCATGC 1 cut(s) 591
SseBI AGGCCT 2 cut(s) 468, 1127
SsiI CCGC 6 cut(s) 62, 576, 1750, 2100, 2416, 3032
SspMI CTAG 4 cut(s) 902, 1062, 1322, 1688
SstI GAGCTC 1 cut(s) 23
StuI AGGCCT 2 cut(s) 468, 1127
StyD4I CCNGG 7 cut(s) 360, 615, 1545, 1823, 1940, 2425, 2832
StyI CCWWGG 6 cut(s) 649, 786, 1080, 1200, 2712, 2922
TaaI ACNGT 8 cut(s) 50, 511, 1306, 1477, 1786, 2051, 2662, 2767
TaiI ACGT 3 cut(s) 1499, 1521, 1997
TatI WGTACW 1 cut(s) 2634
TauI GCSGC 1 cut(s) 2419
TfiI GAWTC 8 cut(s) 259, 1036, 1172, 1181, 1534, 1676, 2446, 2574
Tru1I TTAA 8 cut(s) 690, 801, 1385, 2063, 2183, 2271, 2804, 2880
Tru9I TTAA 8 cut(s) 690, 801, 1385, 2063, 2183, 2271, 2804, 2880
TscAI CASTG 7 cut(s) 382, 484, 514, 1309, 1820, 1912, 2148
TseFI GTSAC 3 cut(s) 178, 488, 766
Tsp45I GTSAC 3 cut(s) 178, 488, 766
TspGWI ACGGA 1 cut(s) 1179
TspRI CASTG 7 cut(s) 382, 484, 514, 1309, 1820, 1912, 2148
VneI GTGCAC 2 cut(s) 1317, 1780
VpaK11BI GGWCC 4 cut(s) 1197, 1753, 2485, 2919
XagI CCTNNNNNAGG 1 cut(s) 1976
XapI RAATTY 7 cut(s) 404, 965, 1248, 1342, 1585, 2027, 2558
XceI RCATGY 2 cut(s) 591, 2890
XcmI CCANNNNNNNNNTGG 3 cut(s) 1948, 2232, 2929
XmiI GTMKAC 2 cut(s) 1849, 2541
XmnI GAANNNNTTC 2 cut(s) 1185, 2724
XspI CTAG 4 cut(s) 902, 1062, 1322, 1688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.