Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0002217

Physical Location & Seq

Genomic Coordinates Reverse (-)

79516798 .. 79521891

Gene Structure 5094 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG464600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3261 bp

ATGCTTCCTAGAAAGAGACCTTGTGAAGGAGAAGAAGGAGACGGTGACGTTAACAAGACCACTGGTACCAGCTCGGCGGCTTCTGCGTCTTTGATCAAGAAGCTTCGGATCGAATCCTACGTTGCCGAATCAGCCATCAACAACAGCAGTAACAACAGCAGCAGCAACGGTAGCGGTAGCGTGGTTGAGAACGACGTGCCGATCATGGCTTTGGGAGATTCCAACGCCGGAGATATCGATGAGGATCTGCACAGCCGGCAGCTTGCCGTCTATGGCCGGGAGACGATGAGGAGGCTCTTTGCATCCAATGTTCTGATCTCAGGGGTACAGGGCCTCGGTGCTGAGATTGCCAAAAATCTCATTCTTGCTGGTGTGAAGTCTGTGACCTTGCATGATGAAGGGACTGTGGAGCTTTGGGATTTGTCCAGCAATTTTATTTTTACAGAGGATGATGTTGGTAAGAACAGGGCACTTGCTTCTGTTCAAAAGTTGCAGGAACTCAACAATGCTGTGATTGTTCATACCTTGACAACTCCATTGACTACGGAGCAGCTTTCTGATTTCCAGGCTGTTGTATTCACTGATATCAGTTACGAGAAAGCTATTGAATTCAATGATTATTGTCATAATCATCAGCCGCCAATCGCCTTCATAAGAACCGAAGTTAGAGGCCTTTTTGGTTCTGTATTTTGTGACTTTGGACCTGAATTCACTGTTTTTGATGTTGATGGGGAGGAACCACACACGGGTATTATTGCATCAATCAGCAATGACAACCCTGCTATTGTGTCATGTGTCGATGATGAAAGACTTGAATTTCAGGATGGGGATCTTGTTGTGTTCTCAGAAGTTCATGGAATGACAGAACTAAATGATGGAAAGCCAAGGAAGATAAAAAATGCTAGGGCTTACTCATTTACTCTTGAGGAGGATACCTCAGGTTTTGGTACCTATGTGAAAGGTGGCATTGTCACACAAGTGAAGCAGCCAAAAGTGTTGAACTTCAAGCCATTGGGGGAAGCACTCAAGAATCCCGGTGATTTTCTTCTCAGTGATTTCTCCAAGTTTGATCGCCCTCCTCTCCTACACTTGGCATTTCAAGCACTGGACAAATTTGTTTTTGAGTTGGGACGATTCCCTGTTGCTGGTTCAGAAGAGGATGCTCAGAAGCTTATATCTGTTGCTACTAACATTAATGATAAACTAGGAGATGGCAGGCTGGAAGATCTTAATCCAAAACTTTTGCGCCACTTCGCCTTTGGTGCAAAGGCGGTACTAAGTCCCATGGCTGCCATGTTTGGTGGTATTGTTGGTCAAGAGGTTGTAAAAGCTTGCTCCGGAAAGTTTCATCCACTCTTCCAGTTCTTCTACTTTGACTCAGTAGAATCACTTCCAACGGAGCCACTGGATTCCAGTGATTTGAAACCATTAAATAGCCGTTATGATGCACAGATTTCAGTTTTTGGGTCCAAGCTACAGAAGAAACTGGAGGATGCCAAAGTATTCATGGTTGGATCTGGTGCACTTGGATGTGAGTTGTTGAAAAATGTTGCCTTGATGGGAGTTTCATGTGGCAACCAAGGGAAGCTAACAATCACTGATGATGATGTAATTGAGAAGAGTAACCTCAGTAGGCAGTTCCTTTTCCGTGATTGGAACATTGGGCAGGCAAAGTCTACAGTTGCCGCTTCTGCTGCTGCATCAATAAATCCCCGTCTCAACATTGTAGCGTTGCAGAATAGAGTTGGTCCTGAAACTGAGAATGTGTTTGATGATACCTACTGGGAGAATTTAAGTGTTGTTATTAATGCATTAGATAATGTCAATGCCAGGCTCTATGTTGATCAGCGGTGCTTATATTTCCAGAAGGCACTTCTTGAGTCGGGTACTCTGGGTGCGAAATGCAACACTCAGATGGTTATTCCTCACCTAACAGAAAACTATGGTGCCTCTAGAGATCCTCCAGAGAAACAAGCACCCATGTGTACTGTCCACTCATTTCCACACAACATTGATCACTGTCTGACATGGGCTCGATCTGAGTTTGAGGGGTTGCTTGAGAAAACTCCAGCGGAAGTGAATGCATATTTATCCAAGCCAAGTGAATATACTGCTGCAATGAGTAATGCTGGGGATGCACAGGCCAGGGACACCTTGGAGCGTGTTCTTGAGTGCCTTTCCAGAGAAAGATGTGAAACATTCCAAGATTGTATTGCCTGGGCGCGCCTCAAGTTTGAAGATTATTTTTCCAACCGGGTGAAACAGCTGACATATACTTTCCCTGAAGATGCTGCAACTAGTACTGGGGCTCCATTCTGGTCTGCCCCCAAGCGATTCCCCCGTGCACTGCAGTTCTCAGCTAATGATCCCGGTCATCTCCATTTTGTCATGGCAGCATCCATACTCAGGGCAGACACATTTGGTATCCCAATCCCCGACTGGGTTAGGAACTCTAAGAAGTTGTCTGAAGCTGTTGAGAAAGTGGAAGTCCCAGACTTTCAGCCCAAGAAAGATGCAAAAATAGTGACAGATGATAAGGCCACCAATTTAACTCCTCAATCTATAGATGATGCCCAGGTTATTAATGAATTGATTATCAAGTTAGAGCATTGTCGAGAGAAACTCCCACCAGGGTTCCGGATGAAACCGATTCAATTTGAGAAGGATGATGATACCAATTACCATATGGATATGATAGCTGGGCTTGCCAATATGAGGGCTAGGAATTACAGCATTCCTGAGGTGGACAAGCTGAAAGCCAAGTTTATTGCCGGTAGGATTATCCCTGCAATTGCAACCTCCACTGCAATGGCCACAGGACTTGTGTGCCTGGAACTTTACAAGGTTCTGGATGGCGACCACAAACTGGAAGACTACAGAAATACATTTGCAAACCTAGCACTACCTTTGTTCTCCATGGCTGAGCCGGTACCTCCCAAGGTCATTAAGCACAAGGACATGAGCTGGACAGTGTGGGATAGGTGGATCCTGAGGGGCAATCCTACTCTGAGGGAACTTCTCCAGTGGCTTAAGGATAAGGGACTGAATGCTTATAGCATCTCTTGTGGAAGCTCTCTGCTTTATAACAGCATGTTTGCTCGACACAAAGACCGAATGGGCAAGAAGTTGGTGGATCTGGCTAAGGACGTGGCGAAGGTGGAAATTCCCCCATATCGTTGCCACTTGGATGTTGTTGTTGCTTGCGAGGATGATGAGGACAATGACATTGACATCCCATTGGTGTCGGTTTACTTCCGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030054 GO:0032446 GO:0033554 GO:0036211 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 ko05012 map04120 map05012

Pfam Domains

Protein Families

E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1086

Amino Acids

120.49

Weight (kDa)

5.24

Isoelectric Point (pI)

32.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	84 - 459	2.1e-27	ThiF family
E1_FCCH	PF16190	258 - 328	8e-29	Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB	PF16191	329 - 399	8.8e-25	Ubiquitin-activating enzyme E1 four-helix bundle
ThiF	PF00899	481 - 973	5.4e-59	ThiF family
UBA_E1_SCCH	PF10585	668 - 921	1.3e-87	Ubiquitin-activating enzyme, SCCH domain
E1_UFD	PF09358	992 - 1081	8.7e-24	Ubiquitin fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30110 AT5G06460
fragaria_vesca	FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_6g10930 FvH4_6g10930 FvH4_6g10930
malus_domestica	MD04G1163500.v1.1 MD11G1065700.v1.1 MD12G1177300.v1.1
prunus_persica	Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G282900_v2.0.a1
pyrus_communis	pycom04g14620 pycom11g05570 pycom12g16790
rosa_chinensis	RchiOBHm_Chr3g0462201 RchiOBHm_Chr5g0070911
rosa_laevigata	RLG00000024878 RLG00000036170
rosa_multiflora	Rmu_sc0002944.1_g000004 Rmu_sc0005970.1_g000009 Rmu_sc0006715.1_g000003
rosa_roxburghii	Rroxscaffold_1G00010290 Rroxscaffold_6G00417690
rosa_rugosa	Rorug03G0054900 Rorug05G0408500 Rorug05G0408500 Rorug05G0408500
rosa_samantha	Rh3CG119500 Rh5AG464600 Rh5DG494900
rosa_wichuraiana	Rw3G009590 Rw5G043220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3084
Acc65I	GGTACC	3 cut(s)	65, 947, 2929
AccB1I	GGYRCC	4 cut(s)	65, 947, 1946, 2929
AccB7I	CCANNNNNTGG	1 cut(s)	2866
AccI	GTMKAC	1 cut(s)	1676
AccII	CGCG	1 cut(s)	2224
AccIII	TCCGGA	2 cut(s)	1337, 2637
AciI	CCGC	7 cut(s)	77, 174, 638, 1271, 1686, 1849, 2072
AclWI	GGATC	9 cut(s)	116, 252, 837, 1522, 1952, 2360, 2980, 2993, 3141
AcoI	YGGCCR	2 cut(s)	274, 2811
AcsI	RAATTY	6 cut(s)	608, 707, 815, 1112, 1789, 3162
AcuI	CTGAAG	2 cut(s)	2304, 2487
AfaI	GTAC	8 cut(s)	67, 327, 949, 1275, 1888, 1987, 2302, 2931
AfiI	CCNNNNNNNGG	8 cut(s)	26, 746, 1090, 1145, 2406, 2440, 2715, 2866
AflII	CTTAAG	1 cut(s)	3029
AhlI	ACTAGT	1 cut(s)	2297
AjiI	CACGTC	2 cut(s)	196, 3148
AjnI	CCWGG	7 cut(s)	564, 1829, 2144, 2216, 2574, 2629, 2829
AleI	CACNNNNGTG	2 cut(s)	977, 1981
Alw21I	GWGCWC	2 cut(s)	1525, 2347
Alw26I	GTCTC	4 cut(s)	10, 33, 275, 1721
Alw44I	GTGCAC	2 cut(s)	1521, 2343
AlwI	GGATC	9 cut(s)	116, 252, 837, 1522, 1952, 2360, 2980, 2993, 3141
Aor13HI	TCCGGA	2 cut(s)	1337, 2637
AoxI	GGCC	6 cut(s)	274, 331, 670, 2142, 2538, 2811
ApaLI	GTGCAC	2 cut(s)	1521, 2343
ApoI	RAATTY	6 cut(s)	608, 707, 815, 1112, 1789, 3162
AscI	GGCGCGCC	1 cut(s)	2222
AseI	ATTAAT	3 cut(s)	1194, 1806, 2583
Asp700I	GAANNNNTTC	1 cut(s)	1389
Asp718I	GGTACC	3 cut(s)	65, 947, 2929
AspLEI	GCGC	3 cut(s)	1248, 2224, 2226
AspS9I	GGNCC	4 cut(s)	331, 701, 1467, 1748
AsuC2I	CCSGG	4 cut(s)	278, 1035, 2255, 2370
AsuHPI	GGTGA	4 cut(s)	56, 1049, 1919, 2269
AvaII	GGWCC	3 cut(s)	701, 1467, 1748
AxyI	CCTNAGG	3 cut(s)	937, 2739, 2990
BaeGI	GKGCMC	3 cut(s)	472, 1525, 2347
BaeI	ACNNNNGTAYC	2 cut(s)	939, 972
BalI	TGGCCA	1 cut(s)	2813
BamHI	GGATCC	1 cut(s)	2985
BanI	GGYRCC	4 cut(s)	65, 947, 1946, 2929
BanII	GRGCYC	2 cut(s)	2035, 2311
BarI	GAAGNNNNNNTAC	2 cut(s)	1374, 1406
BbsI	GAAGAC	1 cut(s)	2877
Bbv12I	GWGCWC	2 cut(s)	1525, 2347
BccI	CCATC	8 cut(s)	143, 722, 818, 869, 1205, 1552, 1909, 2846
BceAI	ACGGC	2 cut(s)	251, 1422
BciT130I	CCWGG	7 cut(s)	566, 1831, 2146, 2218, 2576, 2631, 2831
BciVI	GTATCC	2 cut(s)	925, 2435
BclI	TGATCA	3 cut(s)	93, 1843, 2014
BcnI	CCSGG	4 cut(s)	278, 1035, 2255, 2370
BcoDI	GTCTC	4 cut(s)	10, 33, 275, 1721
BcuI	ACTAGT	1 cut(s)	2297
BfaI	CTAG	7 cut(s)	9, 903, 1205, 1953, 2298, 2721, 2897
BfmI	CTRYAG	5 cut(s)	1475, 1677, 2348, 2562, 2875
BfrI	CTTAAG	1 cut(s)	3029
BfuI	GTATCC	2 cut(s)	925, 2435
BglII	AGATCT	1 cut(s)	1225
BlpI	GCTNAGC	1 cut(s)	2922
BmcAI	AGTACT	1 cut(s)	2302
Bme18I	GGWCC	3 cut(s)	701, 1467, 1748
BmgBI	CACGTC	2 cut(s)	196, 3148
BmgT120I	GGNCC	4 cut(s)	331, 701, 1467, 1748
BmrI	ACTGGG	3 cut(s)	1792, 2313, 2449
BmuI	ACTGGG	3 cut(s)	1792, 2313, 2449
BpiI	GAAGAC	1 cut(s)	2877
BplI	GAGNNNNNCTC	4 cut(s)	920, 952, 2607, 2639
BpmI	CTGGAG	4 cut(s)	1508, 1947, 2052, 3005
Bpu10I	CCTNAGC	1 cut(s)	3141
Bpu1102I	GCTNAGC	1 cut(s)	2922
BpuEI	CTTGAG	6 cut(s)	944, 1010, 1898, 2078, 2189, 2213
BpuMI	CCSGG	4 cut(s)	278, 1035, 2255, 2370
Bsa29I	ATCGAT	1 cut(s)	237
BsaBI	GATNNNNATC	3 cut(s)	243, 828, 1230
BsaI	GGTCTC	1 cut(s)	10
BsaWI	WCCGGW	2 cut(s)	1337, 2637
BsaXI	ACNNNNNCTCC	2 cut(s)	2293, 2323
Bsc4I	CCNNNNNNNGG	8 cut(s)	26, 746, 1090, 1145, 2406, 2440, 2715, 2866
Bse118I	RCCGGY	3 cut(s)	255, 2771, 2926
Bse21I	CCTNAGG	3 cut(s)	937, 2739, 2990
Bse3DI	GCAATG	3 cut(s)	775, 2124, 2814
Bse8I	GATNNNNATC	3 cut(s)	243, 828, 1230
BseAI	TCCGGA	2 cut(s)	1337, 2637
BseBI	CCWGG	7 cut(s)	566, 1831, 2146, 2218, 2576, 2631, 2831
BseCI	ATCGAT	1 cut(s)	237
BseJI	GATNNNNATC	3 cut(s)	243, 828, 1230
BseLI	CCNNNNNNNGG	8 cut(s)	26, 746, 1090, 1145, 2406, 2440, 2715, 2866
BseMI	GCAATG	3 cut(s)	775, 2124, 2814
BsePI	GCGCGC	1 cut(s)	2222
BseRI	GAGGAG	4 cut(s)	304, 941, 1068, 2544
BseSI	GKGCMC	3 cut(s)	472, 1525, 2347
BseYI	CCCAGC	2 cut(s)	2129, 2699
BsgI	GTGCAG	1 cut(s)	233
Bsh1236I	CGCG	1 cut(s)	2224
BshFI	GGCC	6 cut(s)	276, 333, 672, 2144, 2540, 2813
BshNI	GGYRCC	4 cut(s)	65, 947, 1946, 2929
BshVI	ATCGAT	1 cut(s)	237
BsiHKAI	GWGCWC	2 cut(s)	1525, 2347
BslFI	GGGAC	6 cut(s)	415, 1143, 1266, 2162, 2474, 3054
BslI	CCNNNNNNNGG	8 cut(s)	26, 746, 1090, 1145, 2406, 2440, 2715, 2866
BsmAI	GTCTC	4 cut(s)	10, 33, 275, 1721
BsmBI	CGTCTC	3 cut(s)	33, 275, 1721
BsmFI	GGGAC	6 cut(s)	415, 1143, 1266, 2162, 2474, 3054
BsmI	GAATGC	3 cut(s)	2086, 2733, 3052
BsnI	GGCC	6 cut(s)	276, 333, 672, 2144, 2540, 2813
Bso31I	GGTCTC	1 cut(s)	10
Bsp1286I	GDGCHC	5 cut(s)	472, 1525, 2035, 2311, 2347
Bsp13I	TCCGGA	2 cut(s)	1337, 2637
Bsp1720I	GCTNAGC	1 cut(s)	2922
Bsp19I	CCATGG	2 cut(s)	1284, 2916
BspACI	CCGC	7 cut(s)	77, 174, 638, 1271, 1686, 1849, 2072
BspANI	GGCC	6 cut(s)	276, 333, 672, 2144, 2540, 2813
BspDI	ATCGAT	1 cut(s)	237
BspEI	TCCGGA	2 cut(s)	1337, 2637
BspFNI	CGCG	1 cut(s)	2224
BspMAI	CTGCAG	1 cut(s)	2352
BspPI	GGATC	9 cut(s)	116, 252, 837, 1522, 1952, 2360, 2980, 2993, 3141
BspT107I	GGYRCC	4 cut(s)	65, 947, 1946, 2929
BspTI	CTTAAG	1 cut(s)	3029
BspTNI	GGTCTC	1 cut(s)	10
BsrDI	GCAATG	3 cut(s)	775, 2124, 2814
BsrFI	RCCGGY	3 cut(s)	255, 2771, 2926
BssAI	RCCGGY	3 cut(s)	255, 2771, 2926
BssHII	GCGCGC	1 cut(s)	2222
BssT1I	CCWWGG	6 cut(s)	884, 1284, 1579, 2154, 2916, 2937
Bst2UI	CCWGG	7 cut(s)	566, 1831, 2146, 2218, 2576, 2631, 2831
Bst4CI	ACNGT	8 cut(s)	44, 170, 406, 715, 1681, 1990, 2021, 2971
Bst6I	CTCTTC	3 cut(s)	1149, 1361, 1613
BstAFI	CTTAAG	1 cut(s)	3029
BstC8I	GCNNGC	8 cut(s)	257, 264, 1217, 1333, 1668, 2224, 2706, 3202
BstDSI	CCRYGG	2 cut(s)	1284, 2916
BstENI	CCTNNNNNAGG	1 cut(s)	24
BstFNI	CGCG	1 cut(s)	2224
BstHHI	GCGC	3 cut(s)	1248, 2224, 2226
BstMAI	GTCTC	4 cut(s)	10, 33, 275, 1721
BstNI	CCWGG	7 cut(s)	566, 1831, 2146, 2218, 2576, 2631, 2831
BstNSI	RCATGY	1 cut(s)	3094
BstSFI	CTRYAG	5 cut(s)	1475, 1677, 2348, 2562, 2875
BstSLI	GKGCMC	3 cut(s)	472, 1525, 2347
BstUI	CGCG	1 cut(s)	2224
BstV2I	GAAGAC	1 cut(s)	2877
BstX2I	RGATCY	7 cut(s)	244, 829, 1225, 1514, 1957, 2985, 3133
BstXI	CCANNNNNNTGG	1 cut(s)	2809
BstYI	RGATCY	7 cut(s)	244, 829, 1225, 1514, 1957, 2985, 3133
Bsu15I	ATCGAT	1 cut(s)	237
Bsu36I	CCTNAGG	3 cut(s)	937, 2739, 2990
BsuI	GTATCC	2 cut(s)	925, 2435
BsuRI	GGCC	6 cut(s)	276, 333, 672, 2144, 2540, 2813
BsuTUI	ATCGAT	1 cut(s)	237
BtgI	CCRYGG	2 cut(s)	1284, 2916
BtrI	CACGTC	2 cut(s)	196, 3148
BtsI	GCAGTG	2 cut(s)	2345, 2802
Cac8I	GCNNGC	8 cut(s)	257, 264, 1217, 1333, 1668, 2224, 2706, 3202
CfoI	GCGC	3 cut(s)	1248, 2224, 2226
Cfr10I	RCCGGY	3 cut(s)	255, 2771, 2926
Cfr13I	GGNCC	4 cut(s)	331, 701, 1467, 1748
ClaI	ATCGAT	1 cut(s)	237
CseI	GACGC	1 cut(s)	75
Csp6I	GTAC	8 cut(s)	66, 326, 948, 1274, 1887, 1986, 2301, 2930
CspCI	CAANNNNNGTGG	2 cut(s)	2803, 2838
CviQI	GTAC	8 cut(s)	66, 326, 948, 1274, 1887, 1986, 2301, 2930
EaeI	YGGCCR	2 cut(s)	274, 2811
Eam1104I	CTCTTC	3 cut(s)	1149, 1361, 1613
EarI	CTCTTC	3 cut(s)	1149, 1361, 1613
Eco130I	CCWWGG	6 cut(s)	884, 1284, 1579, 2154, 2916, 2937
Eco147I	AGGCCT	1 cut(s)	672
Eco24I	GRGCYC	2 cut(s)	2035, 2311
Eco31I	GGTCTC	1 cut(s)	10
Eco32I	GATATC	2 cut(s)	235, 586
Eco47I	GGWCC	3 cut(s)	701, 1467, 1748
Eco57I	CTGAAG	2 cut(s)	2304, 2487
Eco81I	CCTNAGG	3 cut(s)	937, 2739, 2990
EcoNI	CCTNNNNNAGG	1 cut(s)	24
EcoO109I	RGGNCCY	1 cut(s)	331
EcoRI	GAATTC	2 cut(s)	608, 707
EcoRII	CCWGG	7 cut(s)	564, 1829, 2144, 2216, 2574, 2629, 2829
EcoRV	GATATC	2 cut(s)	235, 586
EcoT14I	CCWWGG	6 cut(s)	884, 1284, 1579, 2154, 2916, 2937
EcoT22I	ATGCAT	2 cut(s)	1813, 2086
EcoT38I	GRGCYC	2 cut(s)	2035, 2311
ErhI	CCWWGG	6 cut(s)	884, 1284, 1579, 2154, 2916, 2937
Esp3I	CGTCTC	3 cut(s)	33, 275, 1721
FaqI	GGGAC	6 cut(s)	415, 1143, 1266, 2162, 2474, 3054
FauNDI	CATATG	1 cut(s)	2685
FbaI	TGATCA	3 cut(s)	93, 1843, 2014
FblI	GTMKAC	1 cut(s)	1676
FriOI	GRGCYC	2 cut(s)	2035, 2311
FspBI	CTAG	7 cut(s)	9, 903, 1205, 1953, 2298, 2721, 2897
GlaI	GCGC	3 cut(s)	1247, 2223, 2225
GsaI	CCCAGC	2 cut(s)	2133, 2703
GsuI	CTGGAG	4 cut(s)	1508, 1947, 2052, 3005
HaeIII	GGCC	6 cut(s)	276, 333, 672, 2144, 2540, 2813
HgaI	GACGC	1 cut(s)	75
HhaI	GCGC	3 cut(s)	1248, 2224, 2226
Hin6I	GCGC	3 cut(s)	1246, 2222, 2224
HinP1I	GCGC	3 cut(s)	1246, 2222, 2224
HincII	GTYRAC	1 cut(s)	52
HindII	GTYRAC	1 cut(s)	52
HindIII	AAGCTT	3 cut(s)	101, 1169, 1329
HpaI	GTTAAC	1 cut(s)	52
HphI	GGTGA	4 cut(s)	56, 1049, 1919, 2269
Hpy166II	GTNNAC	8 cut(s)	52, 1523, 1677, 1986, 1993, 2345, 2746, 3250
Hpy8I	GTNNAC	8 cut(s)	52, 1523, 1677, 1986, 1993, 2345, 2746, 3250
Hpy99I	CGWCG	1 cut(s)	197
HpyAV	CCTTC	7 cut(s)	20, 29, 392, 658, 1861, 2656, 3148
HpyCH4III	ACNGT	8 cut(s)	44, 170, 406, 715, 1681, 1990, 2021, 2971
HpyCH4IV	ACGT	4 cut(s)	48, 120, 195, 3147
HpySE526I	ACGT	4 cut(s)	48, 120, 195, 3147
HspAI	GCGC	3 cut(s)	1246, 2222, 2224
Kpn2I	TCCGGA	2 cut(s)	1337, 2637
KpnI	GGTACC	3 cut(s)	69, 951, 2933
KroI	GCCGGC	1 cut(s)	255
KroNI	GCCGGC	1 cut(s)	257
Ksp22I	TGATCA	3 cut(s)	93, 1843, 2014
KspAI	GTTAAC	1 cut(s)	52
LmnI	GCTCC	6 cut(s)	409, 547, 1340, 1399, 2158, 2314
MaeI	CTAG	7 cut(s)	9, 903, 1205, 1953, 2298, 2721, 2897
MaeII	ACGT	4 cut(s)	48, 120, 195, 3147
MaeIII	GTNAC	8 cut(s)	44, 149, 382, 590, 692, 970, 1622, 2524
MfeI	CAATTG	1 cut(s)	2790
MflI	RGATCY	7 cut(s)	244, 829, 1225, 1514, 1957, 2985, 3133
MhlI	GDGCHC	5 cut(s)	472, 1525, 2035, 2311, 2347
MlsI	TGGCCA	1 cut(s)	2813
MluNI	TGGCCA	1 cut(s)	2813
MlyI	GAGTC	2 cut(s)	1370, 1889
MmeI	TCCRAC	4 cut(s)	246, 1418, 1492, 2274
Mox20I	TGGCCA	1 cut(s)	2813
Mph1103I	ATGCAT	2 cut(s)	1813, 2086
MroI	TCCGGA	2 cut(s)	1337, 2637
MroNI	GCCGGC	1 cut(s)	255
MroXI	GAANNNNTTC	1 cut(s)	1389
MscI	TGGCCA	1 cut(s)	2813
MslI	CAYNNNNRTG	6 cut(s)	977, 1528, 1913, 1981, 2807, 3186
Msp20I	TGGCCA	1 cut(s)	2813
MspA1I	CMGCKG	3 cut(s)	1849, 2072, 2266
MspCI	CTTAAG	1 cut(s)	3029
MunI	CAATTG	1 cut(s)	2790
Mva1269I	GAATGC	3 cut(s)	2086, 2733, 3052
MvaI	CCWGG	7 cut(s)	566, 1831, 2146, 2218, 2576, 2631, 2831
MvnI	CGCG	1 cut(s)	2224
NaeI	GCCGGC	1 cut(s)	257
NciI	CCSGG	4 cut(s)	278, 1035, 2255, 2370
NcoI	CCATGG	2 cut(s)	1284, 2916
NdeI	CATATG	1 cut(s)	2685
NgoMIV	GCCGGC	1 cut(s)	255
NmeAIII	GCCGAG	1 cut(s)	53
NmuCI	GTSAC	5 cut(s)	44, 382, 692, 970, 2524
NsiI	ATGCAT	2 cut(s)	1813, 2086
NspI	RCATGY	1 cut(s)	3094
OliI	CACNNNNGTG	2 cut(s)	977, 1981
PalAI	GGCGCGCC	1 cut(s)	2222
PauI	GCGCGC	1 cut(s)	2222
PceI	AGGCCT	1 cut(s)	672
PcsI	WCGNNNNNNNCGW	1 cut(s)	117
PctI	GAATGC	3 cut(s)	2086, 2733, 3052
PdiI	GCCGGC	1 cut(s)	257
PdmI	GAANNNNTTC	1 cut(s)	1389
PfeI	GAWTC	9 cut(s)	113, 128, 218, 1030, 1134, 1385, 1409, 2334, 2650
PflMI	CCANNNNNTGG	1 cut(s)	2866
PleI	GAGTC	2 cut(s)	1370, 1888
PpsI	GAGTC	2 cut(s)	1370, 1888
PshBI	ATTAAT	3 cut(s)	1194, 1806, 2583
PsiI	TTATAA	1 cut(s)	3084
Psp6I	CCWGG	7 cut(s)	564, 1829, 2144, 2216, 2574, 2629, 2829
PspFI	CCCAGC	2 cut(s)	2129, 2699
PspGI	CCWGG	7 cut(s)	564, 1829, 2144, 2216, 2574, 2629, 2829
PspPI	GGNCC	4 cut(s)	331, 701, 1467, 1748
PstI	CTGCAG	1 cut(s)	2352
PsuI	RGATCY	7 cut(s)	244, 829, 1225, 1514, 1957, 2985, 3133
PteI	GCGCGC	1 cut(s)	2222
PvuII	CAGCTG	1 cut(s)	2266
RsaI	GTAC	8 cut(s)	67, 327, 949, 1275, 1888, 1987, 2302, 2931
RsaNI	GTAC	8 cut(s)	66, 326, 948, 1274, 1887, 1986, 2301, 2930
RseI	CAYNNNNRTG	6 cut(s)	977, 1528, 1913, 1981, 2807, 3186
Sau96I	GGNCC	4 cut(s)	331, 701, 1467, 1748
ScaI	AGTACT	1 cut(s)	2302
SchI	GAGTC	2 cut(s)	1370, 1889
SduI	GDGCHC	5 cut(s)	472, 1525, 2035, 2311, 2347
SfcI	CTRYAG	5 cut(s)	1475, 1677, 2348, 2562, 2875
SgsI	GGCGCGCC	1 cut(s)	2222
SinI	GGWCC	3 cut(s)	701, 1467, 1748
SmiMI	CAYNNNNRTG	6 cut(s)	977, 1528, 1913, 1981, 2807, 3186
SmlI	CTYRAG	7 cut(s)	923, 1025, 1877, 2057, 2168, 2228, 3029
SmoI	CTYRAG	7 cut(s)	923, 1025, 1877, 2057, 2168, 2228, 3029
SpeI	ACTAGT	1 cut(s)	2297
SseBI	AGGCCT	1 cut(s)	672
SsiI	CCGC	7 cut(s)	77, 174, 638, 1271, 1686, 1849, 2072
SspMI	CTAG	7 cut(s)	9, 903, 1205, 1953, 2298, 2721, 2897
StuI	AGGCCT	1 cut(s)	672
StyI	CCWWGG	6 cut(s)	884, 1284, 1579, 2154, 2916, 2937
TaaI	ACNGT	8 cut(s)	44, 170, 406, 715, 1681, 1990, 2021, 2971
TaiI	ACGT	4 cut(s)	51, 123, 198, 3150
TaqI	TCGA	6 cut(s)	111, 237, 798, 2035, 2614, 3100
TaqII	GACCGA	1 cut(s)	3126
TatI	WGTACW	2 cut(s)	1985, 2300
TauI	GCSGC	3 cut(s)	80, 640, 1688
TfiI	GAWTC	9 cut(s)	113, 128, 218, 1030, 1134, 1385, 1409, 2334, 2650
TseFI	GTSAC	5 cut(s)	44, 382, 692, 970, 2524
Tsp45I	GTSAC	5 cut(s)	44, 382, 692, 970, 2524
TspGWI	ACGGA	4 cut(s)	560, 1412, 1637, 3245
Van91I	CCANNNNNTGG	1 cut(s)	2866
Vha464I	CTTAAG	1 cut(s)	3029
VneI	GTGCAC	2 cut(s)	1521, 2343
VpaK11BI	GGWCC	3 cut(s)	701, 1467, 1748
VspI	ATTAAT	3 cut(s)	1194, 1806, 2583
XagI	CCTNNNNNAGG	1 cut(s)	24
XapI	RAATTY	6 cut(s)	608, 707, 815, 1112, 1789, 3162
XbaI	TCTAGA	1 cut(s)	1952
XceI	RCATGY	1 cut(s)	3094
XcmI	CCANNNNNNNNNTGG	5 cut(s)	1256, 1504, 2152, 2436, 2683
XmiI	GTMKAC	1 cut(s)	1676
XmnI	GAANNNNTTC	1 cut(s)	1389
XspI	CTAG	7 cut(s)	9, 903, 1205, 1953, 2298, 2721, 2897
ZrmI	AGTACT	1 cut(s)	2302
Zsp2I	ATGCAT	2 cut(s)	1813, 2086

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.