FvH4_6g10930

Ubiquitin-activating enzyme E1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002217
Physical Location & Seq
Forward (+)
6542155 .. 6548274
6120 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10930.t2

Sequence Viewer

Length: 3237 bp
ATGCTTCCTAGAAAGAGACAAGTGGGCGGAGAGGTTGTAGTACAAGAAGACGGGGAACCGAACTTGGCCGACGAATCTCCCATCAAGAAGCTTTGCACCGACGACTCTAAGGGCACCGACTGCAATTCCGGTAACATCAACAGTAGCAGCAGCAGCAGCAGCGACAAGCCGCCGATCATGGCAATGGGGAATAATGGGAATTCTGGGGATATTGATGAGGATCTCCACAGCCGGCAGCTTGCTGTTTATGGCCGTGAGACGATGAGGCGGCTCTTTGCCTCCAACATTCTTGTCTCTGGGATGCAAGGCCTTGGCGCCGAGATTGCAAAGAACCTTGTTCTTGCTGGTGTCAAGTCTGTGACCTTGCATGATGAAGGAGTTGTTGAGTTGTGGGACCTTTCAGGCAATTTCTTTTTCTCCGAGGAAGATATTGGAAAGAATCGGGCCCTTGCCTGTGTCCAAAAATTGCAAGAACTTAACAATGCTGTGCTCATTTCTACTTTGACAACTCAGCTAACTAAAGAGAAACTTTCTGATTTCCAGGCTGTAGTTTTCACTGATATCAGCTTAGACAAAGCAATCGAATTCGATGATTACTGCCATAATCATCAGCCTCCGATCTCTTTTATCAAATCTGAAGTACGGGGTCTTTTTGGTAGTGTGTTTTGTGACTTTGGTCCTGAGTTCACTGTTCTTGATGTTGACGGTGAGGATCCACACACAGGCATAGTTGCATCAATTAGCAATGACAACCCTGCTATGATATCATGTGTCGATGATGAGAGGCTTGAGTTTCAGGACGGGGATTTGGTTTTATTCACTGAAGTCCATGGAATGACCGAGTTGAATGATGGAAAACCAAGGAAAATAAAGAATGCAAGGCCGTACTCATTCACAATTGAGGAGGACACTACAAATTATGGAGCATACGAGAAAGGTGGTATAGTCACACAGGTTAAGCAACCTAAGGTGTTGAAATTTAAGCCCTTGAGAGAAGCAGTCAAGGAACCTGGTGATTTCCTTCTGATTGACTTCTCCAAGTTTGATCGTCCACCTCTCCTGCACTTAGCATTTCAGGCACTGGATAAGTTCATATCAGAGTTGGGACGCTTCCCTGTTGCTGGATCCGAGGATGATGCTACAAAGTTCATATCATTAGTAACTAGCATAAATGATAGCTCAGCAGATGGGAAGCTTGAGGAGATTGATCAGAAGATCCTTCGTCATTTTGCATTTGGTGCTAGGGCTGTGCTGAATCCCATGGCTGCAATGTTTGGTGGTATTGTTGGACAGGAAGTTGTCAAGGCCTGTTCTGCAAAGTTTCACCCCCTATTCCAGTTCTTCTATTTTGATTCGGTTGAATCGCTTCCAACAGAAGCCTCGGACCCTAGTGATTTGAAGCCTCTAAACAGTCGGTATGATGCACAAATTTCAGTATTTGGAGCCAAGTTGCAGAAAAAGCTGGAGGAGTCGAAAGTGTTCACTGTTGGATCTGGTGCACTAGGATGTGAGTTTTTGAAGAATTTAGCTTTGATGGGTGTCGCTTGCGGTCAAAACGGTAAATTAACAATTACAGATGATGATGTCATTGAGAAGAGTAACCTTAGTAGACAATTTCTCTTCCGGGATTGGAACATTGGGCAGGCTAAATCAACAGTTGCTGCTACTGCTGCTGCGCAGATAAACAGTCGTTTCAATATTGAAGCACTGCAGAATCGTGCAAGCCCAGAGTCTGAAAACGTGTTTGATGATACTTTCTGGGAGAATTTGGATGTTGTTATCAATGCTCTGGATAATGTGAATGCCAGGCTTTACATTGATCAGCGATGCTTATATTTCCAGAAGCCACTATTGGAGTCAGGTACCCTAGGTGCCAAGTGCAACACACAGATGGTCATTCCTCACTTGACTGAAAATTATGGAGCATCAAGGGACCCACCTGAAAAGCAAGCACCTATGTGCACAGTTCATTCATTTCCTCACAACATTGACCACTGCTTGACATGGGCCCGATCTGAGTTTGAGGGTCTGCTTGAGAAGGTTCCTGCCGAAGTAAATGCGTACTTGACAAATCCTAGCGAGTACACTACGGCAATGAAGAATGCTGGTGATGCCCAAGCAAGGAATAACTTGGAAAGTGTCATTGAATGCCTTGACAAGGAGAGATGTGAGACATTTCAAGATTGCATTACCTGGGCCCGTCTAAAGTTTGAGGACTATTTCTCCAACCGTGTGAAGCAGTTAACATATACTTTTCCTGAGGATGCTACAACCAGTAGTGGAACCCCATTCTGGTCTGCTCCCAAGCGTTTCCCTCGCCCACTGGTGTTCTCAGTTGATGATCTCAGTCACCTTCAATTTATACTGGCATCGTCCATTCTTCGAGCTGAGACATTCAACATTGCAATTCCTGATTGGGTCAAGTCTACTCAAAAGTTTGCTGAGGCTGTTAACAACGTGATGGTTCCTGAATTTCAGCCTAAGAAGGATGTGAAGATTGTTACAGATGAGAAAGCTACAATTATTTTACCAGCATCCATCGATGATGCTGCTGTTATCAACGAGTTAGTCATGAAGTTAGAAAAATGCAAGGAGCAGCTCCCACCAGGCTTCAAGATGAACCCTATTCAATTTGAGAAGGATGATGACACGAACTATCATATGGATGTAATAGCTGGATTTGCAAATATGAGGGCAAGGAATTATGGTATCCCTGAAGTCGACAAGCTGAAGGCCAAATTCATTGCCGGTAGAATCATACCTGCAATTGCAACATCTACTGCTCTTGCAACTGGTCTTGTCTGCTTGGAGCTGTACAAGGTTCTGGCCGGAGGGCACAAGATAGAGGACTACCGAAACACCTTTGCCAACCTTGCTCTTCCTCTGTTCTCCATGGCTGAACCAGTGCCTCCTAAAGTCATCAAGCACCAAGATATGAGCTGGACAGTTTGGGACAGGTGGAGTATAAAGGACAACCCAACTCTGAAGCAGCTTCTTAATTGGCTCAAGGAAAAGGGCTTAAACGCTTACAGCATCTCTTATGGCAGTTGCCTTCTTTATAACAGCATGTTCCCCAAGCACAGAGAGCGCATGGACAAGCATATGGTGGATTTGGCTAGGGATGTGGCAAAGGCAGAGCTGCCCCCATTTCGGAACCACTTTGATGTGGTGGTGGCCTGCGAAGACGATGAGGATAATGATATTGACATCCCTCAGATCTCCATTTACTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko05012 map04120 map05012
Pfam Domains
Protein Families
E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1079

Amino Acids

120.26

Weight (kDa)

5.05

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 76 - 440 1.8e-24 ThiF family
E1_FCCH PF16190 250 - 320 7.8e-29 Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB PF16191 321 - 391 1.2e-24 Ubiquitin-activating enzyme E1 four-helix bundle
ThiF PF00899 473 - 965 3.9e-61 ThiF family
UBA_E1_SCCH PF10585 660 - 913 7.1e-86 Ubiquitin-activating enzyme, SCCH domain
E1_UFD PF09358 984 - 1073 1.3e-24 Ubiquitin fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3060
Acc16I TGCGCA 1 cut(s) 1677
Acc36I ACCTGC 1 cut(s) 2770
Acc65I GGTACC 1 cut(s) 1862
AccB1I GGYRCC 4 cut(s) 113, 314, 1862, 1871
AccI GTMKAC 3 cut(s) 1609, 2426, 2721
AciI CCGC 4 cut(s) 27, 170, 268, 1548
AclWI GGATC 7 cut(s) 228, 707, 720, 1119, 1132, 1210, 1498
AcoI YGGCCR 3 cut(s) 66, 250, 2826
AcsI RAATTY 8 cut(s) 199, 584, 977, 1428, 1522, 1765, 2471, 2738
AcuI CTGAAG 5 cut(s) 657, 843, 2736, 2750, 3005
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 7 cut(s) 42, 642, 887, 1864, 2063, 2084, 2816
AfiI CCNNNNNNNGG 6 cut(s) 722, 1121, 2121, 2158, 2292, 3150
AflIII ACRYGT 1 cut(s) 1740
AjnI CCWGG 5 cut(s) 540, 1009, 1805, 2192, 2605
AjuI GAANNNNNNNTTGG 2 cut(s) 1835, 1867
AleI CACNNNNGTG 1 cut(s) 1957
Alw21I GWGCWC 3 cut(s) 492, 1501, 1964
Alw26I GTCTC 5 cut(s) 10, 251, 298, 2165, 2384
Alw44I GTGCAC 2 cut(s) 1497, 1960
AlwI GGATC 7 cut(s) 228, 707, 720, 1119, 1132, 1210, 1498
AlwNI CAGNNNCTG 3 cut(s) 1081, 1661, 1733
ApaI GGGCCC 3 cut(s) 448, 2011, 2200
ApaLI GTGCAC 2 cut(s) 1497, 1960
ApoI RAATTY 8 cut(s) 199, 584, 977, 1428, 1522, 1765, 2471, 2738
Asp700I GAANNNNTTC 2 cut(s) 1365, 1478
Asp718I GGTACC 1 cut(s) 1862
AspA2I CCTAGG 1 cut(s) 1867
AspLEI GCGC 3 cut(s) 317, 1678, 3090
AsuC2I CCSGG 1 cut(s) 1625
AsuHPI GGTGA 5 cut(s) 719, 1025, 1316, 2120, 2342
AvaII GGWCC 4 cut(s) 394, 677, 1384, 1933
AvrII CCTAGG 1 cut(s) 1867
AxyI CCTNAGG 2 cut(s) 966, 2259
BaeGI GKGCMC 7 cut(s) 116, 448, 1501, 1964, 2011, 2200, 2838
BamHI GGATCC 2 cut(s) 712, 1124
BanI GGYRCC 4 cut(s) 113, 314, 1862, 1871
BanII GRGCYC 3 cut(s) 448, 2011, 2200
BbsI GAAGAC 2 cut(s) 54, 3189
Bbv12I GWGCWC 3 cut(s) 492, 1501, 1964
BbvCI CCTCAGC 1 cut(s) 2442
BccI CCATC 7 cut(s) 89, 845, 1181, 1528, 1885, 2455, 2546
BceAI ACGGC 3 cut(s) 237, 868, 2106
BcgI CGANNNNNNTGC 6 cut(s) 1118, 1152, 2039, 2073, 2531, 2565
BciT130I CCWGG 5 cut(s) 542, 1011, 1807, 2194, 2607
BciVI GTATCC 1 cut(s) 2720
BclI TGATCA 2 cut(s) 1207, 1819
BcnI CCSGG 1 cut(s) 1625
BcoDI GTCTC 5 cut(s) 10, 251, 298, 2165, 2384
BfaI CTAG 8 cut(s) 9, 1164, 1242, 1389, 1502, 1868, 2076, 3117
BfmI CTRYAG 2 cut(s) 546, 1709
BfoI RGCGCY 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 2770
BfuI GTATCC 1 cut(s) 2720
BglII AGATCT 1 cut(s) 3216
BlnI CCTAGG 1 cut(s) 1867
BlpI GCTNAGC 1 cut(s) 1180
Bme1390I CCNGG 6 cut(s) 542, 1011, 1625, 1807, 2194, 2607
Bme18I GGWCC 4 cut(s) 394, 677, 1384, 1933
BmrFI CCNGG 6 cut(s) 542, 1011, 1625, 1807, 2194, 2607
BoxI GACNNNNGTC 1 cut(s) 675
BpiI GAAGAC 2 cut(s) 54, 3189
BpmI CTGGAG 1 cut(s) 1484
Bpu10I CCTNAGC 1 cut(s) 2442
Bpu1102I GCTNAGC 1 cut(s) 1180
BpuEI CTTGAG 5 cut(s) 809, 1009, 1217, 2054, 2990
BpuMI CCSGG 1 cut(s) 1625
Bsa29I ATCGAT 1 cut(s) 2541
BsaBI GATNNNNATC 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 2207, 2237
Bsc4I CCNNNNNNNGG 6 cut(s) 722, 1121, 2121, 2158, 2292, 3150
Bse118I RCCGGY 2 cut(s) 231, 2747
Bse1I ACTGG 7 cut(s) 1086, 1336, 2274, 2328, 2370, 2797, 2903
Bse21I CCTNAGG 2 cut(s) 966, 2259
Bse3DI GCAATG 6 cut(s) 189, 751, 1275, 2100, 2400, 2742
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 5 cut(s) 542, 1011, 1807, 2194, 2607
BseCI ATCGAT 1 cut(s) 2541
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 6 cut(s) 722, 1121, 2121, 2158, 2292, 3150
BseMI GCAATG 6 cut(s) 189, 751, 1275, 2100, 2400, 2742
BseNI ACTGG 7 cut(s) 1086, 1336, 2274, 2328, 2370, 2797, 2903
BseRI GAGGAG 3 cut(s) 917, 1214, 1481
BseSI GKGCMC 7 cut(s) 116, 448, 1501, 1964, 2011, 2200, 2838
BsgI GTGCAG 1 cut(s) 1046
BshNI GGYRCC 4 cut(s) 113, 314, 1862, 1871
BshVI ATCGAT 1 cut(s) 2541
BsiHKAI GWGCWC 3 cut(s) 492, 1501, 1964
BsiSI CCGG 5 cut(s) 129, 232, 1624, 2748, 2829
BslFI GGGAC 4 cut(s) 407, 1119, 1946, 2966
BslI CCNNNNNNNGG 6 cut(s) 722, 1121, 2121, 2158, 2292, 3150
BsmAI GTCTC 5 cut(s) 10, 251, 298, 2165, 2384
BsmBI CGTCTC 1 cut(s) 251
BsmFI GGGAC 4 cut(s) 407, 1119, 1946, 2966
BsmI GAATGC 4 cut(s) 880, 1807, 2107, 2153
Bsp120I GGGCCC 3 cut(s) 444, 2007, 2196
Bsp1286I GDGCHC 8 cut(s) 116, 448, 492, 1501, 1964, 2011, 2200, 2838
Bsp1407I TGTACA 1 cut(s) 2814
Bsp1720I GCTNAGC 1 cut(s) 1180
Bsp19I CCATGG 3 cut(s) 829, 1260, 2892
BspACI CCGC 4 cut(s) 27, 170, 268, 1548
BspDI ATCGAT 1 cut(s) 2541
BspHI TCATGA 1 cut(s) 2571
BspMAI CTGCAG 1 cut(s) 1713
BspMI ACCTGC 1 cut(s) 2770
BspPI GGATC 7 cut(s) 228, 707, 720, 1119, 1132, 1210, 1498
BspQI GCTCTTC 1 cut(s) 2883
BspT107I GGYRCC 4 cut(s) 113, 314, 1862, 1871
BsrDI GCAATG 6 cut(s) 189, 751, 1275, 2100, 2400, 2742
BsrFI RCCGGY 2 cut(s) 231, 2747
BsrGI TGTACA 1 cut(s) 2814
BsrI ACTGG 7 cut(s) 1086, 1336, 2274, 2328, 2370, 2797, 2903
BssAI RCCGGY 2 cut(s) 231, 2747
BssNI GRCGYC 1 cut(s) 315
BssT1I CCWWGG 6 cut(s) 310, 829, 860, 1260, 1867, 2892
Bst2UI CCWGG 5 cut(s) 542, 1011, 1807, 2194, 2607
Bst6I CTCTTC 3 cut(s) 1589, 1625, 2883
BstACI GRCGYC 1 cut(s) 315
BstAPI GCANNNNNTGC 2 cut(s) 120, 1238
BstAUI TGTACA 1 cut(s) 2814
BstC8I GCNNGC 7 cut(s) 233, 240, 1546, 1644, 1723, 1950, 3178
BstDSI CCRYGG 3 cut(s) 829, 1260, 2892
BstENI CCTNNNNNAGG 1 cut(s) 2156
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 3 cut(s) 317, 1678, 3090
BstMAI GTCTC 5 cut(s) 10, 251, 298, 2165, 2384
BstNI CCWGG 5 cut(s) 542, 1011, 1807, 2194, 2607
BstNSI RCATGY 1 cut(s) 3070
BstPAI GACNNNNGTC 1 cut(s) 675
BstSCI CCNGG 6 cut(s) 540, 1009, 1623, 1805, 2192, 2605
BstSFI CTRYAG 2 cut(s) 546, 1709
BstSLI GKGCMC 7 cut(s) 116, 448, 1501, 1964, 2011, 2200, 2838
BstV2I GAAGAC 2 cut(s) 54, 3189
BstX2I RGATCY 6 cut(s) 220, 712, 1124, 1215, 1490, 3216
BstYI RGATCY 6 cut(s) 220, 712, 1124, 1215, 1490, 3216
Bsu15I ATCGAT 1 cut(s) 2541
Bsu36I CCTNAGG 2 cut(s) 966, 2259
BsuI GTATCC 1 cut(s) 2720
BsuTUI ATCGAT 1 cut(s) 2541
BtgI CCRYGG 3 cut(s) 829, 1260, 2892
BtgZI GCGATG 1 cut(s) 1840
BtsI GCAGTG 2 cut(s) 1706, 1993
BtsIMutI CAGTG 9 cut(s) 555, 687, 819, 1079, 1482, 1706, 1993, 2321, 2910
BveI ACCTGC 1 cut(s) 2770
Cac8I GCNNGC 7 cut(s) 233, 240, 1546, 1644, 1723, 1950, 3178
CaiI CAGNNNCTG 3 cut(s) 1081, 1661, 1733
CciI TCATGA 1 cut(s) 2571
CfoI GCGC 3 cut(s) 317, 1678, 3090
Cfr10I RCCGGY 2 cut(s) 231, 2747
ClaI ATCGAT 1 cut(s) 2541
CseI GACGC 1 cut(s) 1116
CsiI ACCWGGT 1 cut(s) 1009
Csp6I GTAC 7 cut(s) 41, 641, 886, 1863, 2062, 2083, 2815
CviQI GTAC 7 cut(s) 41, 641, 886, 1863, 2062, 2083, 2815
DinI GGCGCC 1 cut(s) 316
EaeI YGGCCR 3 cut(s) 66, 250, 2826
Eam1104I CTCTTC 3 cut(s) 1589, 1625, 2883
EarI CTCTTC 3 cut(s) 1589, 1625, 2883
EciI GGCGGA 1 cut(s) 42
Eco130I CCWWGG 6 cut(s) 310, 829, 860, 1260, 1867, 2892
Eco147I AGGCCT 2 cut(s) 309, 1307
Eco24I GRGCYC 3 cut(s) 448, 2011, 2200
Eco32I GATATC 2 cut(s) 562, 765
Eco47I GGWCC 4 cut(s) 394, 677, 1384, 1933
Eco57I CTGAAG 5 cut(s) 657, 843, 2736, 2750, 3005
Eco81I CCTNAGG 2 cut(s) 966, 2259
EcoNI CCTNNNNNAGG 1 cut(s) 2156
EcoO109I RGGNCCY 3 cut(s) 394, 445, 1933
EcoRI GAATTC 2 cut(s) 199, 584
EcoRII CCWGG 5 cut(s) 540, 1009, 1805, 2192, 2605
EcoRV GATATC 2 cut(s) 562, 765
EcoT14I CCWWGG 6 cut(s) 310, 829, 860, 1260, 1867, 2892
EcoT38I GRGCYC 3 cut(s) 448, 2011, 2200
EgeI GGCGCC 1 cut(s) 316
EheI GGCGCC 1 cut(s) 316
ErhI CCWWGG 6 cut(s) 310, 829, 860, 1260, 1867, 2892
Esp3I CGTCTC 1 cut(s) 251
FalI AAGNNNNNCTT 2 cut(s) 513, 545
FaqI GGGAC 4 cut(s) 407, 1119, 1946, 2966
FauNDI CATATG 2 cut(s) 2661, 3102
FbaI TGATCA 2 cut(s) 1207, 1819
FblI GTMKAC 3 cut(s) 1609, 2426, 2721
FriOI GRGCYC 3 cut(s) 448, 2011, 2200
FspBI CTAG 8 cut(s) 9, 1164, 1242, 1389, 1502, 1868, 2076, 3117
FspI TGCGCA 1 cut(s) 1677
GlaI GCGC 3 cut(s) 316, 1677, 3089
GsuI CTGGAG 1 cut(s) 1484
HaeII RGCGCY 1 cut(s) 318
HapII CCGG 5 cut(s) 129, 232, 1624, 2748, 2829
HgaI GACGC 1 cut(s) 1116
HhaI GCGC 3 cut(s) 317, 1678, 3090
Hin1I GRCGYC 1 cut(s) 315
Hin6I GCGC 3 cut(s) 315, 1676, 3088
HinP1I GCGC 3 cut(s) 315, 1676, 3088
HincII GTYRAC 4 cut(s) 703, 2244, 2452, 2722
HindII GTYRAC 4 cut(s) 703, 2244, 2452, 2722
HindIII AAGCTT 2 cut(s) 89, 1193
HpaI GTTAAC 2 cut(s) 2244, 2452
HpaII CCGG 5 cut(s) 129, 232, 1624, 2748, 2829
HphI GGTGA 5 cut(s) 719, 1025, 1316, 2120, 2342
Hpy99I CGWCG 2 cut(s) 74, 104
HpyAV CCTTC 9 cut(s) 368, 1031, 1229, 2032, 2363, 2479, 2632, 2725, 3062
HpyCH4IV ACGT 2 cut(s) 1740, 2457
HpySE526I ACGT 2 cut(s) 1740, 2457
Hsp92I GRCGYC 1 cut(s) 315
HspAI GCGC 3 cut(s) 315, 1676, 3088
KasI GGCGCC 1 cut(s) 314
KflI GGGWCCC 1 cut(s) 1933
KpnI GGTACC 1 cut(s) 1866
KroI GCCGGC 1 cut(s) 231
KroNI GCCGGC 1 cut(s) 233
Ksp22I TGATCA 2 cut(s) 1207, 1819
KspAI GTTAAC 2 cut(s) 2244, 2452
LguI GCTCTTC 1 cut(s) 2883
LmnI GCTCC 7 cut(s) 923, 1442, 1922, 2305, 2593, 2604, 2809
MabI ACCWGGT 1 cut(s) 1009
MaeI CTAG 8 cut(s) 9, 1164, 1242, 1389, 1502, 1868, 2076, 3117
MaeII ACGT 2 cut(s) 1740, 2457
MaeIII GTNAC 8 cut(s) 131, 358, 668, 946, 1159, 1598, 2348, 2500
MfeI CAATTG 2 cut(s) 897, 2766
MflI RGATCY 6 cut(s) 220, 712, 1124, 1215, 1490, 3216
MhlI GDGCHC 8 cut(s) 116, 448, 492, 1501, 1964, 2011, 2200, 2838
Mly113I GGCGCC 1 cut(s) 315
MlyI GAGTC 4 cut(s) 98, 1478, 1739, 1865
MmeI TCCRAC 5 cut(s) 306, 1267, 1394, 1468, 2250
MroNI GCCGGC 1 cut(s) 231
MroXI GAANNNNTTC 2 cut(s) 1365, 1478
MseI TTAA 8 cut(s) 477, 957, 981, 1565, 2243, 2451, 2997, 3020
MslI CAYNNNNRTG 6 cut(s) 182, 1504, 1889, 1957, 2664, 3162
MspI CCGG 5 cut(s) 129, 232, 1624, 2748, 2829
MspR9I CCNGG 6 cut(s) 542, 1011, 1625, 1807, 2194, 2607
MunI CAATTG 2 cut(s) 897, 2766
Mva1269I GAATGC 4 cut(s) 880, 1807, 2107, 2153
MvaI CCWGG 5 cut(s) 542, 1011, 1807, 2194, 2607
NaeI GCCGGC 1 cut(s) 233
NarI GGCGCC 1 cut(s) 315
NciI CCSGG 1 cut(s) 1625
NcoI CCATGG 3 cut(s) 829, 1260, 2892
NdeI CATATG 2 cut(s) 2661, 3102
NgoMIV GCCGGC 1 cut(s) 231
NmeAIII GCCGAG 1 cut(s) 343
NmuCI GTSAC 4 cut(s) 358, 668, 946, 2348
NsbI TGCGCA 1 cut(s) 1677
NspI RCATGY 1 cut(s) 3070
OliI CACNNNNGTG 1 cut(s) 1957
PagI TCATGA 1 cut(s) 2571
PceI AGGCCT 2 cut(s) 309, 1307
PciSI GCTCTTC 1 cut(s) 2883
PctI GAATGC 4 cut(s) 880, 1807, 2107, 2153
PdiI GCCGGC 1 cut(s) 233
PdmI GAANNNNTTC 2 cut(s) 1365, 1478
PfeI GAWTC 7 cut(s) 74, 439, 1255, 1352, 1361, 1714, 2754
PfoI TCCNGGA 1 cut(s) 1623
PleI GAGTC 4 cut(s) 98, 1477, 1738, 1864
PluTI GGCGCC 1 cut(s) 318
PpsI GAGTC 4 cut(s) 98, 1477, 1738, 1864
PpuMI RGGWCCY 2 cut(s) 394, 1933
PshAI GACNNNNGTC 1 cut(s) 675
PsiI TTATAA 1 cut(s) 3060
Psp5II RGGWCCY 2 cut(s) 394, 1933
Psp6I CCWGG 5 cut(s) 540, 1009, 1805, 2192, 2605
PspGI CCWGG 5 cut(s) 540, 1009, 1805, 2192, 2605
PspOMI GGGCCC 3 cut(s) 444, 2007, 2196
PspPPI RGGWCCY 2 cut(s) 394, 1933
PstI CTGCAG 1 cut(s) 1713
PstNI CAGNNNCTG 3 cut(s) 1081, 1661, 1733
PsuI RGATCY 6 cut(s) 220, 712, 1124, 1215, 1490, 3216
RsaI GTAC 7 cut(s) 42, 642, 887, 1864, 2063, 2084, 2816
RsaNI GTAC 7 cut(s) 41, 641, 886, 1863, 2062, 2083, 2815
RseI CAYNNNNRTG 6 cut(s) 182, 1504, 1889, 1957, 2664, 3162
SalI GTCGAC 1 cut(s) 2720
SapI GCTCTTC 1 cut(s) 2883
SaqAI TTAA 8 cut(s) 477, 957, 981, 1565, 2243, 2451, 2997, 3020
SchI GAGTC 4 cut(s) 98, 1478, 1739, 1865
ScrFI CCNGG 6 cut(s) 542, 1011, 1625, 1807, 2194, 2607
SduI GDGCHC 8 cut(s) 116, 448, 492, 1501, 1964, 2011, 2200, 2838
SexAI ACCWGGT 1 cut(s) 1009
SfcI CTRYAG 2 cut(s) 546, 1709
SfoI GGCGCC 1 cut(s) 316
SinI GGWCC 4 cut(s) 394, 677, 1384, 1933
SmiMI CAYNNNNRTG 6 cut(s) 182, 1504, 1889, 1957, 2664, 3162
SmlI CTYRAG 5 cut(s) 788, 988, 1196, 2033, 3005
SmoI CTYRAG 5 cut(s) 788, 988, 1196, 2033, 3005
SseBI AGGCCT 2 cut(s) 309, 1307
SsiI CCGC 4 cut(s) 27, 170, 268, 1548
SspDI GGCGCC 1 cut(s) 314
SspI AATATT 1 cut(s) 1699
SspMI CTAG 8 cut(s) 9, 1164, 1242, 1389, 1502, 1868, 2076, 3117
StuI AGGCCT 2 cut(s) 309, 1307
StyD4I CCNGG 6 cut(s) 540, 1009, 1623, 1805, 2192, 2605
StyI CCWWGG 6 cut(s) 310, 829, 860, 1260, 1867, 2892
TaiI ACGT 2 cut(s) 1743, 2460
TaqI TCGA 7 cut(s) 582, 588, 774, 1472, 2383, 2541, 2721
TaqII GACCGA 1 cut(s) 854
TatI WGTACW 3 cut(s) 40, 2082, 2814
TauI GCSGC 2 cut(s) 172, 271
TfiI GAWTC 7 cut(s) 74, 439, 1255, 1352, 1361, 1714, 2754
Tru1I TTAA 8 cut(s) 477, 957, 981, 1565, 2243, 2451, 2997, 3020
Tru9I TTAA 8 cut(s) 477, 957, 981, 1565, 2243, 2451, 2997, 3020
TscAI CASTG 9 cut(s) 562, 694, 826, 1086, 1489, 1713, 2000, 2328, 2910
TseFI GTSAC 4 cut(s) 358, 668, 946, 2348
Tsp45I GTSAC 4 cut(s) 358, 668, 946, 2348
TspDTI ATGAA 9 cut(s) 387, 1081, 1138, 1958, 1962, 2111, 2588, 2633, 2731
TspRI CASTG 9 cut(s) 562, 694, 826, 1086, 1489, 1713, 2000, 2328, 2910
VneI GTGCAC 2 cut(s) 1497, 1960
VpaK11BI GGWCC 4 cut(s) 394, 677, 1384, 1933
XagI CCTNNNNNAGG 1 cut(s) 2156
XapI RAATTY 8 cut(s) 199, 584, 977, 1428, 1522, 1765, 2471, 2738
XceI RCATGY 1 cut(s) 3070
XmaJI CCTAGG 1 cut(s) 1867
XmiI GTMKAC 3 cut(s) 1609, 2426, 2721
XmnI GAANNNNTTC 2 cut(s) 1365, 1478
XspI CTAG 8 cut(s) 9, 1164, 1242, 1389, 1502, 1868, 2076, 3117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.