Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002217

Physical Location & Seq

Genomic Coordinates Forward (+)

1969960 .. 1974527

Gene Structure 4568 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G06460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3234 bp

ATGGAACCATTCGTTGTTAAGGAGAACATCATCGCATCTGCTTCTTCGCCGATGAAGAAGCGACGGATCGATCACACTGAGTCAGCTGATGGTTCTGCGATTAACGCTTCTAACTCCAGTAGCATCGGTTTGAACAACAGCATCGGTGGTAACGATACGGTGATGAGCATGGCGGAGTTTGGTAACGACAACTCCAACAATCAGGAGATCGATGAAGATCTTCATAGCAGACAGCTCGCCGTTTACGGCCGTGAGACTATGAGGAAGCTTTTCGCTTCCAACGTTCTCATCTCTGGGATGCAGGGCCTTGGTGTTGAGATTGCCAAGAATATTATTCTAGCTGGTGTGAAGTCAGTGACCCTTCACGATGAAAATGTGGTTGAGCTATGGGACCTATCTAGTAACTTTGTTTTCACGGAGGAGGACATTGGTAAGAATAGAGCACTTGCTTCTGTTCACAAGTTGCAAGAACTCAACAATGCTGTGGCTGTCTCAACTTTGACTGGGAAGTTAACCAAAGAGCAGCTTTCTGATTTCCAGGTTGTGGTATTTGTGGATATCTCTTTTGAGAAAGCGACAGAGATTGATGATTATTGTCACAGTCATCAGCCTCCTATTGCATTCATCAAGGCTGATGTTAGGGGGCTGTTTGGGTCTTTGTTTTGTGATTTTGGACCTCATTTTACTGTTCTTGATGTTGATGGGGAGGAACCTCACTCTGGCATCATCGCTTCTGTTAGCAATGAAAATCCTGGATTTGTTTCGTGTGTCGATGATGAGAGGCTCGAGTTTGAAGATGGAAATTTGGTTGTTTTCTCTGAAGTTGAGGGAATGACAGAGCTAAATGATGGAAAACCAAGGAAGATAAAAAATGTCAAGCCATTTTCATTTACCCTGGAGGAAGACACCTCTAGCTATGGGCAATATATGAAGGGTGGCATTGTGACTCAGGTTAAACAACCAAAGGTGTTGAACTTTAAACCTCTTAGAGAAGCACTTAAAGATCCAGGCGACTTTCTTCTAAGTGACTTTTCAAAGTTTGACCGACCACCTCTTCTCCACTTAGCATTCCAAGCACTTGATAGGTTTTCGTCTCAAGCAGGCCGATTTCCGTTTGCTGGTTCAGAAGAAGATGCTCAGAAACTAGTGGAAATTGCAGTTGACATTAATGAGGGTTTGGGTGATGCAAGGTTGGAAGATGTCAACTCTAAACTGTTGCGGCATTTGGCCTTTGGATCCAGGGCTGTTCTGAATCCTATGGCTGCAATGTTTGGTGGCATTGTTGGGCAGGAGGTTGTCAAGGCATGCTCTGGGAAATTCCATCCTATCTTTCAGTTCTTTTACTTTGATTCGGTGGAGTCACTCCCCAAGGAACCTCTTGATGCATCAGAGTTTAGACCACAAAATAGCCGTTACGATGCACAGATCTCTGTATTTGGGTCTACTCTTCAGAAGAAACTCGAGGATGCCAGAGTTTTTGTTGTAGGGGCTGGTGCTCTTGGATGCGAGTTCTTAAAGAACTTGGCTTTGATGGGTGTGTCATGTGGCACCCAAGGGAAGCTAACTGTCACCGATGATGATGTAATTGAGAAGAGCAACCTGAGTCGTCAGTTTCTTTTTCGAGATTGGAACATAGGACAGGCTAAATCCACAGTTGCCGCAACAGCAGCAGCTGGAATAAATTCTCGCCTCAACATTGATGCTCTGCAAAATCGTGTTGGACCTGAGACCGAGAATGTCTTTGACGACAGCTTCTGGGAGAATTTGACAGTTGTGGTGAACGCTTTGGACAATGTCACTGCAAGGCTTTATGTTGATTCAAGGTGTGTGTATTTCCAGAAGCCTCTCCTCGAGTCAGGAACCCTAGGTGCAAAGTGCAACACACAGATGGTTATCCCTCACCTCACTGAGAACTATGGTGCCTCGAGAGACCCACCAGAGAAACAGGCTCCAATGTGCACCGTGCACTCATTTCCTCACAACATTGATCATTGCTTAACATGGGCTCGCTCTGAATTTGAGGGTTTGCTTGAGAAAACACCAGCTGAAGTTAATGCATACCTCTCAGATCCTGTTGAATACATGAAAGCGATGAGAACTGCTGGTGATGCCCAAGCAAGGGATACTTTGGGACGTGTTGTTGAGTGCCTCGAGAAAGAAAAGTGCAATTCATTCCAGGATTGCATAACCTGGGCTCGTCTGAGGTTTGAGGACTACTTCGCCAACCGTGTGAAGCAGTTATGTTACACATTTCCTGAGGATGCTGCTACTAGTACAGGAGCTCCGTTCTGGTCTGCCCCAAAGAGATTTCCTCGCCCACTCCAATTCTCCTCAACCGACCTGAGCCATATCAACTTTGTCATGGCTGCATCTATCTTGCGAGCAGAGACATTCGGAATCCCTACTCCTGAGTGGGCTAAAACCCGAGCAGGACTAGCCGAGGCTGTGGAAAGAGTAATTGTCCCCGACTTTGAGCCGAAGAAAGATGCAACTATTGTGACAGATGAGAAGGCTACAACTTTGTCTACAGCTTCAGTCGATGATGCAGCAGTAATCGATGAACTAAACGCAAAACTAGTGCGGTGCAGGATGAGCTTGCAGCCAGAGTTCAGGATGAAGGCAATCCAGTTTGAAAAGGACGATGATACCAACTACCACATGGATATGATCGCTGGGCTAGCCAACATGAGAGCAAGGAACTACAGTGTTCCCGAGGTGGACAAGCTTAAAGCCAAGTTCATAGCCGGAAGAATCATACCCGCGATTGCGACATCAACTGCAATGGCAACAGGTTTTGTTTGCCTTGAGATGTACAAGGTCTTAGACGGGTCACACAAAGTAGAAGACTACAGAAACACATTTGCTAACTTAGCACTTCCGCTCTTCTCCATGGCTGAGCCGGTTCCACCTAAAGTGGTGAAGCACCAAGACCAAAGCTGGACTGTTTGGGACCGGTGGGTGATGAGGGGAAACCCGACCCTAAGGGAGTTGCTAGACTGGCTAAAAGAGAAAGGGCTAAACGCATACAGCATATCTTGCGGAAGCAGTTTACTGTACAACAGTATGTTCTCAAGGCATAAAGAGAGGATGAACAGAAGAGTGGTGGATCTTGCAAGAGACGTGGCTGGGGTTGAATTGCCTGCTTATAGACGTCACGTGGATGTTGTAGTCGCTTGTGAAGATGATAATGATGCTGACGTTGATATCCCTCTCGTATCAGTCTACTTCGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0030054 GO:0032446 GO:0033554 GO:0036211 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 ko05012 map04120 map05012

Pfam Domains

Protein Families

E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1077

Amino Acids

119.62

Weight (kDa)

5.13

Isoelectric Point (pI)

35.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	75 - 453	3.3e-27	ThiF family
E1_FCCH	PF16190	249 - 319	1.8e-28	Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB	PF16191	320 - 390	1.4e-26	Ubiquitin-activating enzyme E1 four-helix bundle
ThiF	PF00899	472 - 964	7.8e-61	ThiF family
UBA_E1_SCCH	PF10585	659 - 912	6.4e-84	Ubiquitin-activating enzyme, SCCH domain
E1_UFD	PF09358	983 - 1072	1e-23	Ubiquitin fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30110 AT5G06460
fragaria_vesca	FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_3g39370 FvH4_6g10930 FvH4_6g10930 FvH4_6g10930
malus_domestica	MD04G1163500.v1.1 MD11G1065700.v1.1 MD12G1177300.v1.1
prunus_persica	Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G051900_v2.0.a1 Prupe.6G282900_v2.0.a1
pyrus_communis	pycom04g14620 pycom11g05570 pycom12g16790
rosa_chinensis	RchiOBHm_Chr3g0462201 RchiOBHm_Chr5g0070911
rosa_laevigata	RLG00000024878 RLG00000036170
rosa_multiflora	Rmu_sc0002944.1_g000004 Rmu_sc0005970.1_g000009 Rmu_sc0006715.1_g000003
rosa_roxburghii	Rroxscaffold_1G00010290 Rroxscaffold_6G00417690
rosa_rugosa	Rorug03G0054900 Rorug05G0408500 Rorug05G0408500 Rorug05G0408500
rosa_samantha	Rh3CG119500 Rh5AG464600 Rh5DG494900
rosa_wichuraiana	Rw3G009590 Rw5G043220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3154
AccB1I	GGYRCC	2 cut(s)	1547, 1919
AccB7I	CCANNNNNTGG	1 cut(s)	544
AccBSI	CCGCTC	1 cut(s)	2881
AccI	GTMKAC	3 cut(s)	1442, 2525, 3222
AccII	CGCG	1 cut(s)	2762
AciI	CCGC	7 cut(s)	173, 1219, 1659, 2581, 2760, 2879, 3039
AclI	AACGTT	1 cut(s)	282
AclWI	GGATC	6 cut(s)	74, 998, 1230, 1243, 2063, 3114
AcoI	YGGCCR	1 cut(s)	247
AcsI	RAATTY	5 cut(s)	802, 1316, 1681, 1762, 2015
AcuI	CTGAAG	4 cut(s)	840, 1433, 2067, 2517
AcvI	CACGTG	1 cut(s)	3157
AcyI	GRCGYC	1 cut(s)	3151
AfaI	GTAC	3 cut(s)	2275, 2813, 3056
AfiI	CCNNNNNNNGG	5 cut(s)	544, 719, 1118, 2118, 2119
AflIII	ACRYGT	1 cut(s)	2134
AgeI	ACCGGT	1 cut(s)	2952
AgsI	TTSAA	8 cut(s)	133, 794, 973, 1035, 1821, 2078, 2633, 3134
AhlI	ACTAGT	3 cut(s)	1144, 2270, 2575
AjiI	CACGTC	2 cut(s)	2135, 3121
AjnI	CCWGG	7 cut(s)	537, 751, 894, 1006, 1238, 2175, 2189
Alw21I	GWGCWC	5 cut(s)	445, 1498, 1961, 1968, 2284
Alw26I	GTCTC	7 cut(s)	248, 496, 1098, 1721, 1923, 2381, 3111
Alw44I	GTGCAC	2 cut(s)	1957, 1964
AlwI	GGATC	6 cut(s)	74, 998, 1230, 1243, 2063, 3114
AlwNI	CAGNNNCTG	3 cut(s)	1673, 1755, 2072
Ama87I	CYCGRG	7 cut(s)	785, 1460, 1850, 1924, 2150, 2424, 2711
AoxI	GGCC	4 cut(s)	247, 304, 1102, 1227
ApaLI	GTGCAC	2 cut(s)	1957, 1964
ApeKI	GCWGC	8 cut(s)	523, 1262, 1667, 1670, 2264, 2366, 2546, 2599
ApoI	RAATTY	5 cut(s)	802, 1316, 1681, 1762, 2015
AseI	ATTAAT	1 cut(s)	1167
AsiGI	ACCGGT	1 cut(s)	2952
Asp700I	GAANNNNTTC	3 cut(s)	219, 269, 1681
AspA2I	CCTAGG	1 cut(s)	1864
AspS9I	GGNCC	5 cut(s)	304, 391, 674, 1721, 2950
AsuHPI	GGTGA	8 cut(s)	172, 1193, 1561, 1789, 1892, 2117, 2929, 2971
AsuNHI	GCTAGC	1 cut(s)	2677
AvaI	CYCGRG	7 cut(s)	785, 1460, 1850, 1924, 2150, 2424, 2711
AvaII	GGWCC	4 cut(s)	391, 674, 1721, 2950
AvrII	CCTAGG	1 cut(s)	1864
AxyI	CCTNAGG	2 cut(s)	2256, 2981
BaeGI	GKGCMC	2 cut(s)	1961, 1968
BamHI	GGATCC	1 cut(s)	1235
BanI	GGYRCC	2 cut(s)	1547, 1919
BanII	GRGCYC	3 cut(s)	2008, 2197, 2284
BbrPI	CACGTG	1 cut(s)	3157
BbsI	GAAGAC	2 cut(s)	909, 2850
Bbv12I	GWGCWC	5 cut(s)	445, 1498, 1961, 1968, 2284
BbvI	GCAGC	8 cut(s)	535, 1249, 1679, 1682, 2251, 2353, 2558, 2611
BccI	CCATC	7 cut(s)	83, 695, 791, 842, 1329, 1525, 1882
BceAI	ACGGC	4 cut(s)	224, 234, 262, 1395
BcgI	CGANNNNNNTGC	2 cut(s)	217, 251
BciT130I	CCWGG	7 cut(s)	539, 753, 896, 1008, 1240, 2177, 2191
BciVI	GTATCC	1 cut(s)	2116
BclI	TGATCA	1 cut(s)	1987
BcoDI	GTCTC	7 cut(s)	248, 496, 1098, 1721, 1923, 2381, 3111
BcuI	ACTAGT	3 cut(s)	1144, 2270, 2575
BfmI	CTRYAG	3 cut(s)	2526, 2701, 2848
BfuI	GTATCC	1 cut(s)	2116
BglII	AGATCT	2 cut(s)	217, 1425
BlnI	CCTAGG	1 cut(s)	1864
BlpI	GCTNAGC	1 cut(s)	2895
Bme1390I	CCNGG	7 cut(s)	539, 753, 896, 1008, 1240, 2177, 2191
Bme18I	GGWCC	4 cut(s)	391, 674, 1721, 2950
BmeT110I	CYCGRG	7 cut(s)	785, 1460, 1850, 1924, 2150, 2424, 2711
BmgBI	CACGTC	2 cut(s)	2135, 3121
BmgT120I	GGNCC	5 cut(s)	304, 391, 674, 1721, 2950
BmrFI	CCNGG	7 cut(s)	539, 753, 896, 1008, 1240, 2177, 2191
BmrI	ACTGGG	1 cut(s)	513
BmtI	GCTAGC	1 cut(s)	2681
BmuI	ACTGGG	1 cut(s)	513
BpiI	GAAGAC	2 cut(s)	909, 2850
BplI	GAGNNNNNCTC	2 cut(s)	2296, 2328
BpmI	CTGGAG	2 cut(s)	100, 917
Bpu10I	CCTNAGC	1 cut(s)	2342
Bpu1102I	GCTNAGC	1 cut(s)	2895
BpuEI	CTTGAG	4 cut(s)	1080, 2051, 2825, 3055
Bsa29I	ATCGAT	3 cut(s)	69, 210, 2556
BsaAI	YACGTR	1 cut(s)	3157
BsaBI	GATNNNNATC	2 cut(s)	216, 1892
BsaHI	GRCGYC	1 cut(s)	3151
BsaI	GGTCTC	2 cut(s)	1721, 1923
BsaWI	WCCGGW	1 cut(s)	2952
BsaXI	ACNNNNNCTCC	6 cut(s)	176, 206, 1041, 1071, 2266, 2296
Bsc4I	CCNNNNNNNGG	5 cut(s)	544, 719, 1118, 2118, 2119
Bse118I	RCCGGY	2 cut(s)	2899, 2952
Bse1I	ACTGG	4 cut(s)	117, 508, 2626, 3002
Bse21I	CCTNAGG	2 cut(s)	2256, 2981
Bse3DI	GCAATG	4 cut(s)	748, 1272, 1990, 2787
Bse8I	GATNNNNATC	2 cut(s)	216, 1892
BseBI	CCWGG	7 cut(s)	539, 753, 896, 1008, 1240, 2177, 2191
BseCI	ATCGAT	3 cut(s)	69, 210, 2556
BseGI	GGATG	9 cut(s)	303, 1321, 1471, 1508, 2266, 2595, 2619, 3093, 3166
BseJI	GATNNNNATC	2 cut(s)	216, 1892
BseLI	CCNNNNNNNGG	5 cut(s)	544, 719, 1118, 2118, 2119
BseMI	GCAATG	4 cut(s)	748, 1272, 1990, 2787
BseNI	ACTGG	4 cut(s)	117, 508, 2626, 3002
BseRI	GAGGAG	3 cut(s)	434, 1838, 2320
BseSI	GKGCMC	2 cut(s)	1961, 1968
BseX3I	CGGCCG	1 cut(s)	247
BseXI	GCAGC	8 cut(s)	535, 1249, 1679, 1682, 2251, 2353, 2558, 2611
BseYI	CCCAGC	2 cut(s)	2672, 3125
BsgI	GTGCAG	1 cut(s)	2605
Bsh1236I	CGCG	1 cut(s)	2762
Bsh1285I	CGRYCG	1 cut(s)	250
BshFI	GGCC	4 cut(s)	249, 306, 1104, 1229
BshNI	GGYRCC	2 cut(s)	1547, 1919
BshTI	ACCGGT	1 cut(s)	2952
BshVI	ATCGAT	3 cut(s)	69, 210, 2556
BsiEI	CGRYCG	1 cut(s)	250
BsiHKAI	GWGCWC	5 cut(s)	445, 1498, 1961, 1968, 2284
BsiHKCI	CYCGRG	7 cut(s)	785, 1460, 1850, 1924, 2150, 2424, 2711
BsiSI	CCGG	3 cut(s)	2745, 2900, 2953
BslFI	GGGAC	4 cut(s)	404, 2145, 2447, 2963
BslI	CCNNNNNNNGG	5 cut(s)	544, 719, 1118, 2118, 2119
BsmAI	GTCTC	7 cut(s)	248, 496, 1098, 1721, 1923, 2381, 3111
BsmBI	CGTCTC	2 cut(s)	1098, 3111
BsmFI	GGGAC	4 cut(s)	404, 2145, 2447, 2963
BsmI	GAATGC	2 cut(s)	620, 1067
BsnI	GGCC	4 cut(s)	249, 306, 1104, 1229
Bso31I	GGTCTC	2 cut(s)	1721, 1923
BsoBI	CYCGRG	7 cut(s)	785, 1460, 1850, 1924, 2150, 2424, 2711
Bsp1286I	GDGCHC	7 cut(s)	445, 1498, 1961, 1968, 2008, 2197, 2284
Bsp1407I	TGTACA	2 cut(s)	2811, 3054
Bsp1720I	GCTNAGC	1 cut(s)	2895
Bsp19I	CCATGG	1 cut(s)	2889
BspACI	CCGC	7 cut(s)	173, 1219, 1659, 2581, 2760, 2879, 3039
BspANI	GGCC	4 cut(s)	249, 306, 1104, 1229
BspDI	ATCGAT	3 cut(s)	69, 210, 2556
BspFNI	CGCG	1 cut(s)	2762
BspOI	GCTAGC	1 cut(s)	2681
BspPI	GGATC	6 cut(s)	74, 998, 1230, 1243, 2063, 3114
BspQI	GCTCTTC	2 cut(s)	1586, 2888
BspT107I	GGYRCC	2 cut(s)	1547, 1919
BspTNI	GGTCTC	2 cut(s)	1721, 1923
BsrBI	CCGCTC	1 cut(s)	2881
BsrDI	GCAATG	4 cut(s)	748, 1272, 1990, 2787
BsrFI	RCCGGY	2 cut(s)	2899, 2952
BsrGI	TGTACA	2 cut(s)	2811, 3054
BsrI	ACTGG	4 cut(s)	117, 508, 2626, 3002
BssAI	RCCGGY	2 cut(s)	2899, 2952
BssNI	GRCGYC	1 cut(s)	3151
BssT1I	CCWWGG	6 cut(s)	307, 857, 1368, 1552, 1864, 2889
Bst2UI	CCWGG	7 cut(s)	539, 753, 896, 1008, 1240, 2177, 2191
Bst6I	CTCTTC	5 cut(s)	1059, 1452, 1586, 2888, 3091
BstACI	GRCGYC	1 cut(s)	3151
BstAPI	GCANNNNNTGC	1 cut(s)	3036
BstAUI	TGTACA	2 cut(s)	2811, 3054
BstBAI	YACGTR	1 cut(s)	3157
BstC8I	GCNNGC	8 cut(s)	237, 1102, 1306, 2008, 2382, 2597, 2679, 3141
BstDSI	CCRYGG	1 cut(s)	2889
BstF5I	GGATG	9 cut(s)	303, 1321, 1471, 1508, 2266, 2595, 2619, 3093, 3166
BstFNI	CGCG	1 cut(s)	2762
BstMAI	GTCTC	7 cut(s)	248, 496, 1098, 1721, 1923, 2381, 3111
BstMCI	CGRYCG	1 cut(s)	250
BstNI	CCWGG	7 cut(s)	539, 753, 896, 1008, 1240, 2177, 2191
BstNSI	RCATGY	1 cut(s)	1308
BstSCI	CCNGG	7 cut(s)	537, 751, 894, 1006, 1238, 2175, 2189
BstSFI	CTRYAG	3 cut(s)	2526, 2701, 2848
BstSLI	GKGCMC	2 cut(s)	1961, 1968
BstUI	CGCG	1 cut(s)	2762
BstV1I	GCAGC	8 cut(s)	535, 1249, 1679, 1682, 2251, 2353, 2558, 2611
BstV2I	GAAGAC	2 cut(s)	909, 2850
BstX2I	RGATCY	6 cut(s)	217, 1003, 1235, 1425, 2068, 3106
BstYI	RGATCY	6 cut(s)	217, 1003, 1235, 1425, 2068, 3106
BstZI	CGGCCG	1 cut(s)	247
Bsu15I	ATCGAT	3 cut(s)	69, 210, 2556
Bsu36I	CCTNAGG	2 cut(s)	2256, 2981
BsuI	GTATCC	1 cut(s)	2116
BsuRI	GGCC	4 cut(s)	249, 306, 1104, 1229
BsuTUI	ATCGAT	3 cut(s)	69, 210, 2556
BtgI	CCRYGG	1 cut(s)	2889
BtgZI	GCGATG	3 cut(s)	16, 712, 2105
BtrI	CACGTC	2 cut(s)	2135, 3121
BtsCI	GGATG	9 cut(s)	303, 1321, 1471, 1508, 2266, 2595, 2619, 3093, 3166
BtsI	GCAGTG	1 cut(s)	1797
BtsIMutI	CAGTG	5 cut(s)	75, 360, 1797, 1905, 2710
Cac8I	GCNNGC	8 cut(s)	237, 1102, 1306, 2008, 2382, 2597, 2679, 3141
CaiI	CAGNNNCTG	3 cut(s)	1673, 1755, 2072
Cfr10I	RCCGGY	2 cut(s)	2899, 2952
Cfr13I	GGNCC	5 cut(s)	304, 391, 674, 1721, 2950
ClaI	ATCGAT	3 cut(s)	69, 210, 2556
Csp6I	GTAC	3 cut(s)	2274, 2812, 3055
CspAI	ACCGGT	1 cut(s)	2952
CspCI	CAANNNNNGTGG	2 cut(s)	3102, 3137
CviAII	CATG	9 cut(s)	169, 1305, 1542, 2001, 2083, 2362, 2659, 2686, 2890
CviQI	GTAC	3 cut(s)	2274, 2812, 3055
DraI	TTTAAA	1 cut(s)	979
EaeI	YGGCCR	1 cut(s)	247
EagI	CGGCCG	1 cut(s)	247
Eam1104I	CTCTTC	5 cut(s)	1059, 1452, 1586, 2888, 3091
EarI	CTCTTC	5 cut(s)	1059, 1452, 1586, 2888, 3091
EciI	GGCGGA	1 cut(s)	188
Ecl136II	GAGCTC	1 cut(s)	2282
EclXI	CGGCCG	1 cut(s)	247
Eco130I	CCWWGG	6 cut(s)	307, 857, 1368, 1552, 1864, 2889
Eco24I	GRGCYC	3 cut(s)	2008, 2197, 2284
Eco31I	GGTCTC	2 cut(s)	1721, 1923
Eco32I	GATATC	2 cut(s)	559, 3205
Eco47I	GGWCC	4 cut(s)	391, 674, 1721, 2950
Eco52I	CGGCCG	1 cut(s)	247
Eco53kI	GAGCTC	1 cut(s)	2282
Eco57I	CTGAAG	4 cut(s)	840, 1433, 2067, 2517
Eco72I	CACGTG	1 cut(s)	3157
Eco81I	CCTNAGG	2 cut(s)	2256, 2981
Eco88I	CYCGRG	7 cut(s)	785, 1460, 1850, 1924, 2150, 2424, 2711
EcoICRI	GAGCTC	1 cut(s)	2282
EcoO109I	RGGNCCY	2 cut(s)	304, 391
EcoRII	CCWGG	7 cut(s)	537, 751, 894, 1006, 1238, 2175, 2189
EcoRV	GATATC	2 cut(s)	559, 3205
EcoT14I	CCWWGG	6 cut(s)	307, 857, 1368, 1552, 1864, 2889
EcoT22I	ATGCAT	2 cut(s)	1387, 2059
EcoT38I	GRGCYC	3 cut(s)	2008, 2197, 2284
ErhI	CCWWGG	6 cut(s)	307, 857, 1368, 1552, 1864, 2889
Esp3I	CGTCTC	2 cut(s)	1098, 3111
FaeI	CATG	9 cut(s)	172, 1308, 1545, 2004, 2086, 2365, 2662, 2689, 2893
FalI	AAGNNNNNCTT	4 cut(s)	510, 542, 2110, 2142
FaqI	GGGAC	4 cut(s)	404, 2145, 2447, 2963
FatI	CATG	9 cut(s)	168, 1304, 1541, 2000, 2082, 2361, 2658, 2685, 2889
FauI	CCCGC	1 cut(s)	2767
FbaI	TGATCA	1 cut(s)	1987
FblI	GTMKAC	3 cut(s)	1442, 2525, 3222
FokI	GGATG	9 cut(s)	310, 1308, 1478, 1515, 2273, 2602, 2626, 3100, 3173
FriOI	GRGCYC	3 cut(s)	2008, 2197, 2284
GsaI	CCCAGC	2 cut(s)	2676, 3129
GsuI	CTGGAG	2 cut(s)	100, 917
HaeIII	GGCC	4 cut(s)	249, 306, 1104, 1229
HapII	CCGG	3 cut(s)	2745, 2900, 2953
Hin1I	GRCGYC	1 cut(s)	3151
Hin1II	CATG	9 cut(s)	172, 1308, 1545, 2004, 2086, 2365, 2662, 2689, 2893
HincII	GTYRAC	3 cut(s)	513, 1162, 1204
HindII	GTYRAC	3 cut(s)	513, 1162, 1204
HindIII	AAGCTT	2 cut(s)	266, 2723
HpaI	GTTAAC	1 cut(s)	513
HpaII	CCGG	3 cut(s)	2745, 2900, 2953
HphI	GGTGA	8 cut(s)	172, 1193, 1561, 1789, 1892, 2117, 2929, 2971
Hpy99I	CGWCG	1 cut(s)	66
HpyAV	CCTTC	4 cut(s)	371, 925, 2503, 2611
HpyCH4IV	ACGT	6 cut(s)	282, 2134, 3120, 3151, 3156, 3198
HpySE526I	ACGT	6 cut(s)	282, 2134, 3120, 3151, 3156, 3198
Hsp92I	GRCGYC	1 cut(s)	3151
Hsp92II	CATG	9 cut(s)	172, 1308, 1545, 2004, 2086, 2365, 2662, 2689, 2893
Ksp22I	TGATCA	1 cut(s)	1987
KspAI	GTTAAC	1 cut(s)	513
LguI	GCTCTTC	2 cut(s)	1586, 2888
LmnI	GCTCC	3 cut(s)	1954, 2279, 2287
Lsp1109I	GCAGC	8 cut(s)	535, 1249, 1679, 1682, 2251, 2353, 2558, 2611
MaeII	ACGT	6 cut(s)	282, 2134, 3120, 3151, 3156, 3198
MbiI	CCGCTC	1 cut(s)	2881
MflI	RGATCY	6 cut(s)	217, 1003, 1235, 1425, 2068, 3106
MhlI	GDGCHC	7 cut(s)	445, 1498, 1961, 1968, 2008, 2197, 2284
MlyI	GAGTC	5 cut(s)	89, 940, 1367, 1612, 1862
MmeI	TCCRAC	4 cut(s)	219, 303, 1173, 1699
Mph1103I	ATGCAT	2 cut(s)	1387, 2059
MroXI	GAANNNNTTC	3 cut(s)	219, 269, 1681
MslI	CAYNNNNRTG	3 cut(s)	1886, 2663, 3159
MspA1I	CMGCKG	3 cut(s)	86, 1673, 2045
MspI	CCGG	3 cut(s)	2745, 2900, 2953
MspR9I	CCNGG	7 cut(s)	539, 753, 896, 1008, 1240, 2177, 2191
Mva1269I	GAATGC	2 cut(s)	620, 1067
MvaI	CCWGG	7 cut(s)	539, 753, 896, 1008, 1240, 2177, 2191
MvnI	CGCG	1 cut(s)	2762
NcoI	CCATGG	1 cut(s)	2889
NheI	GCTAGC	1 cut(s)	2677
NlaIII	CATG	9 cut(s)	172, 1308, 1545, 2004, 2086, 2365, 2662, 2689, 2893
NmeAIII	GCCGAG	1 cut(s)	2464
NsiI	ATGCAT	2 cut(s)	1387, 2059
NspI	RCATGY	1 cut(s)	1308
PaeI	GCATGC	1 cut(s)	1308
PaeR7I	CTCGAG	5 cut(s)	785, 1460, 1850, 1924, 2150
PciSI	GCTCTTC	2 cut(s)	1586, 2888
PcsI	WCGNNNNNNNCGW	2 cut(s)	150, 279
PctI	GAATGC	2 cut(s)	620, 1067
PdmI	GAANNNNTTC	3 cut(s)	219, 269, 1681
PfeI	GAWTC	5 cut(s)	1252, 1349, 1817, 2397, 2751
PflFI	GACNNNGTC	1 cut(s)	1793
PflMI	CCANNNNNTGG	1 cut(s)	544
PfoI	TCCNGGA	2 cut(s)	751, 2175
PinAI	ACCGGT	1 cut(s)	2952
PleI	GAGTC	5 cut(s)	88, 940, 1366, 1611, 1861
PmaCI	CACGTG	1 cut(s)	3157
PmlI	CACGTG	1 cut(s)	3157
PpsI	GAGTC	5 cut(s)	88, 940, 1366, 1611, 1861
Ppu21I	YACGTR	1 cut(s)	3157
PpuMI	RGGWCCY	1 cut(s)	391
PshBI	ATTAAT	1 cut(s)	1167
Psp124BI	GAGCTC	1 cut(s)	2284
Psp1406I	AACGTT	1 cut(s)	282
Psp5II	RGGWCCY	1 cut(s)	391
Psp6I	CCWGG	7 cut(s)	537, 751, 894, 1006, 1238, 2175, 2189
PspCI	CACGTG	1 cut(s)	3157
PspFI	CCCAGC	2 cut(s)	2672, 3125
PspGI	CCWGG	7 cut(s)	537, 751, 894, 1006, 1238, 2175, 2189
PspPI	GGNCC	5 cut(s)	304, 391, 674, 1721, 2950
PspPPI	RGGWCCY	1 cut(s)	391
PspXI	VCTCGAGB	3 cut(s)	785, 1460, 1850
PstNI	CAGNNNCTG	3 cut(s)	1673, 1755, 2072
PsuI	RGATCY	6 cut(s)	217, 1003, 1235, 1425, 2068, 3106
PsyI	GACNNNGTC	1 cut(s)	1793
PvuII	CAGCTG	3 cut(s)	86, 1673, 2045
RsaI	GTAC	3 cut(s)	2275, 2813, 3056
RsaNI	GTAC	3 cut(s)	2274, 2812, 3055
RseI	CAYNNNNRTG	3 cut(s)	1886, 2663, 3159
SacI	GAGCTC	1 cut(s)	2284
SapI	GCTCTTC	2 cut(s)	1586, 2888
Sau96I	GGNCC	5 cut(s)	304, 391, 674, 1721, 2950
SchI	GAGTC	5 cut(s)	89, 940, 1367, 1612, 1862
ScrFI	CCNGG	7 cut(s)	539, 753, 896, 1008, 1240, 2177, 2191
SduI	GDGCHC	7 cut(s)	445, 1498, 1961, 1968, 2008, 2197, 2284
SfcI	CTRYAG	3 cut(s)	2526, 2701, 2848
Sfr274I	CTCGAG	5 cut(s)	785, 1460, 1850, 1924, 2150
SinI	GGWCC	4 cut(s)	391, 674, 1721, 2950
SlaI	CTCGAG	5 cut(s)	785, 1460, 1850, 1924, 2150
SmiMI	CAYNNNNRTG	3 cut(s)	1886, 2663, 3159
SmlI	CTYRAG	9 cut(s)	785, 1095, 1460, 1850, 1924, 2030, 2150, 2804, 3070
SmoI	CTYRAG	9 cut(s)	785, 1095, 1460, 1850, 1924, 2030, 2150, 2804, 3070
SpeI	ACTAGT	3 cut(s)	1144, 2270, 2575
SphI	GCATGC	1 cut(s)	1308
SsiI	CCGC	7 cut(s)	173, 1219, 1659, 2581, 2760, 2879, 3039
SspI	AATATT	1 cut(s)	331
SstI	GAGCTC	1 cut(s)	2284
StyD4I	CCNGG	7 cut(s)	537, 751, 894, 1006, 1238, 2175, 2189
StyI	CCWWGG	6 cut(s)	307, 857, 1368, 1552, 1864, 2889
TaiI	ACGT	6 cut(s)	285, 2137, 3123, 3154, 3159, 3201
TaqII	GACCGA	2 cut(s)	1059, 1745
TatI	WGTACW	3 cut(s)	2273, 2811, 3054
TauI	GCSGC	2 cut(s)	1222, 1661
TfiI	GAWTC	5 cut(s)	1252, 1349, 1817, 2397, 2751
TscAI	CASTG	5 cut(s)	82, 360, 1804, 1912, 2710
TseI	GCWGC	8 cut(s)	523, 1262, 1667, 1670, 2264, 2366, 2546, 2599
TspGWI	ACGGA	4 cut(s)	79, 431, 1101, 2274
TspRI	CASTG	5 cut(s)	82, 360, 1804, 1912, 2710
Tth111I	GACNNNGTC	1 cut(s)	1793
Van91I	CCANNNNNTGG	1 cut(s)	544
VneI	GTGCAC	2 cut(s)	1957, 1964
VpaK11BI	GGWCC	4 cut(s)	391, 674, 1721, 2950
VspI	ATTAAT	1 cut(s)	1167
XapI	RAATTY	5 cut(s)	802, 1316, 1681, 1762, 2015
XceI	RCATGY	1 cut(s)	1308
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2656
XhoI	CTCGAG	5 cut(s)	785, 1460, 1850, 1924, 2150
XmaJI	CCTAGG	1 cut(s)	1864
XmiI	GTMKAC	3 cut(s)	1442, 2525, 3222
XmnI	GAANNNNTTC	3 cut(s)	219, 269, 1681
ZraI	GACGTC	1 cut(s)	3152
Zsp2I	ATGCAT	2 cut(s)	1387, 2059

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.