RchiOBHm_Chr3g0462201

Ubiquitin-activating enzyme E1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002217
Physical Location & Seq
Forward (+)
9946244 .. 9952285
6042 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42858

Sequence Viewer

Length: 3240 bp
ATGCTTCCTAGAAAGAGACAAGTGGGCGGAGAGGTTGTAGTAGAAGAAGGGGAACCAGATTTCGCCAACGAATCTCCAATCAAGAAGCTTTGCACCGACGAATCTAAGGGCAGTGACTGCAAAAACACTGGTAACAGCAATATCAGCAGCAGCAACAGTAGCAGCAGCGTCAAGCCGCCGATCATGGCGTTAGGAAATGGGAATTCGGGGGATATTGATGAGGATCTCCACAGCCGGCAGCTTGCTGTGTACGGCCGTGAGACGATGAGGCGGCTCTTTGCCTCCAACATTCTTGTCTCTGGGATGCAAGGCCTTGGTGCCGAGATTGCAAAGAACCTTGTTCTTGCCGGTGTCAAGTCTGTGACCTTGCATGATGAAGGAGTTGTTGAGTTGTGGGACCTGTCTGGTAATTTCTTTTTCACTGAAGACGATATTGGAAAGAATCGGGCCCTTGCTTGTGTCCAAAAATTGCAAGAACTTAATAATGCTGTGCTCATTTCAACTTTGACAACTCAGCTGACTAAAGAAAAACTTTCTGATTTCCAGGCTGTAGTTTTCACTGATATCAGCTTAGACAAAGCAATTGAATTCGATGATTACTGCCATAATCATCAGCCTCCAATCTCTTTTATCAAAACTGAAGTACGGGGCCTTTTTGGCAGTGTGTTTTGTGACTTTGGTCCTGAGTTCACTGTTCTTGATGTTGATGGTGAGGATCCACACACAGGCATAGTCGCATCAATTAGCAATGACAACCCTGCTATGATATCATGTGTCGATGATGAGAGGCTTGAGTTTCAGGACGGGGATTTGGTTCTGTTCACTGAAGTCCATGGAATGACCGAGTTGAATGACGGAAAGCCGAGGAAGATTAAGAATGCAAGGCCGTATTCATTCACAATTGAGCAGGACACTACAAATTATGCAGGATATGAGAAAGGTGGTATAGTTACACAGGTTAAGCAACCCAAGGTGTTGAAATTTAAGCCCTTGCGAGAAGCAGTCAAGGAACCTGGTGATTTCCTTCTGATTGACTTCTCCAAGTTTGATCGGCCACCTCTCCTGCACTTGGCATTTCAGGCACTAGATAAGTTTATATCAGAGTTAGGACGTTTCCCTGTTGCTGGATCCGAGGATGATGCTACAAAGTTCATATCATTGGTAACTAGCATAAATGATGGCTCGGCAGATGGGAGACTTGAGGAGATTGATCAGAAGATTCTTCGTCACTTTGCATTTGGTGCTAGGGCTGTGCTGAATCCCATGGCTGCAATGTTTGGTGGTATTGTTGGACAGGAGGTTGTGAAGGCCTGTTCTGCAAAGTTTCACCCTCTTTTCCAGTTTTTCTATTTCGATTCAGTTGAATCACTTCCGACAGAAGCCTTGGACCCTAGTGATTTGAAGCCTCTAAACAGTCGGTATGATGCACAAATTTCAGTATTTGGAGCCAAGCTGCAGCAAAAGCTGGAGGATTCAAAAGTGTTCACTGTTGGGTCTGGTGCACTAGGATGCGAGTTTTTGAAGAATTTAGCTTTGATGGGCGTTGCTTGTGGTCAAGAAGGTAAATTAACAATTACAGATGATGATGTCATTGAGAAGAGTAACCTTAGTAGACAATTTCTCTTCCGGGATTGGAACATTGGGCAGGCTAAATCAACAGTTGCTGCCACTGCTGCTGCACTTATAAACAGTCGTATCAATATTGAAGCACTGCAGAATCGTGCAAGCCCAGAGACTGAAAACGTGTTTGATGATACTTTCTGGGAGAATTTGGATGTTGTTATCAATGCTCTAGATAATGTGAATGCTAGGCTTTACATTGATCAGCGATGCTTGTATTTCCAGAAGCCACTTTTGGAATCAGGTACCCTAGGTGCCAAGTGCAACACACAGATGGTCATTCCTCACTTGACTGAAAATTATGGAGCATCACGGGACCCACCTGAAAAGCAAGCACCTATGTGCACAGTTCATTCATTTCCTCACAACATTGACCACTGCTTGACATGGGCTCGTTCTGAGTTTGAGGGTTTACTTGAGAAGGTCCCAGCTGAAGTAAATGCCTACTTGACAAATCCTAGTGAGTACACTACTGCAATGAAGAATGCTGGTGATGCTCAGGCAAGGAATAACTTGGAAAGTGTCATTGAATGCCTTGACAAGGAGAGATGCGAGACATTTCAAGATTGCATAACCTGGGCCCGACTAAAGTTTGAGGACTACTTTTCCAACCGTGTGAAGCAGTTAACATATACTTTTCCTGAGGATGCTACAACCAGTAGTGGGACCCCATTCTGGTCTGCTCCCAAGCGTTTCCCTCGCCCACTGGTGTTCTCAGTAGATGATCTCAGCCACCTTCAATTTGTACTGGCAGCATCCATACTACGAGCTGAGACATTTAACATTGCAATTCCTGATTGGATCAAGTCTACTCAAAAGTTTGCTGATGCTGTTAACAACGTGATGGTACCTGAATTTCAGCCTAAGAAGGATGTGAAGATTGTAATAGATGAGAAAGCTACAAACATTCTACCGGCATCCATCGATGATGCTGCTGTTATCAACGAGTTAGTTATGAAGTTAGAAAAATGCAGGGAGCAGCTCCCACCAGCCTTCAAGATGAACCCTATTCAATTTGAGAAGGATGATGACACAAACTATCATATGGATGTAATAGCTGGATTTGCAAATATGAGGGCTAGGAACTATAGTATCCCTGAAGTCGACAAGCTGAAGGCCAAATTCATCGCCGGTAGAATCATTCCTGCAATTGCAACATCCACTGCTCTTGCAACTGGTCTTGTCTGCTTGGAGCTGTACAAGGTTCTGGCCGGAGAGCACAAGATAGAGGACTACCGAAACACCTTTGCCAATCTTGCTCTTCCTCTGTTCTCCATGGCTGAACCAGTACCTCCTAAAGTCATCAAGCACCAAGATATGAGCTGGACAGTTTGGGACAGATGGATTATAAAGGACAACCCAACTTTGAAGCAGCTCCTTAATTGGCTCAAAGAAAAGGGCTTAAACGCTTACAGCATCTCTTATGGCAGTTGCCTTCTGTATAACAGCATGTTCCCCAAGCACAGAGAGCGCATGGACAAGCATATGGTGGATTTGGCTAGGGATGTGGCAAAGGCGGAGCTGCCTCCATATCGGAACCACTTTGATGTGGTGGTGGCCTGCGAAGATGATGAGGATAATGATATTGACATCCCTCAGATCTCCATTTACTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 ko05012 map04120 map05012
Pfam Domains
Protein Families
E1_4HB E1_FCCH E1_UFD ThiF UBA_e1_thiolCys

Protein Analysis

1079

Amino Acids

120.54

Weight (kDa)

5.05

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 77 - 451 1.5e-24 ThiF family
E1_FCCH PF16190 251 - 321 4.9e-28 Ubiquitin-activating enzyme E1 FCCH domain
E1_4HB PF16191 322 - 392 1.2e-24 Ubiquitin-activating enzyme E1 four-helix bundle
ThiF PF00899 474 - 966 3.8e-61 ThiF family
UBA_E1_SCCH PF10585 661 - 914 1.7e-86 Ubiquitin-activating enzyme, SCCH domain
E1_UFD PF09358 985 - 1074 6.3e-25 Ubiquitin fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1685, 2969
Acc65I GGTACC 2 cut(s) 1865, 2467
AccB1I GGYRCC 4 cut(s) 317, 1865, 1874, 2467
AccI GTMKAC 3 cut(s) 1612, 2429, 2724
AciI CCGC 4 cut(s) 27, 176, 271, 3137
AclWI GGATC 6 cut(s) 231, 710, 723, 1122, 1135, 2429
AcoI YGGCCR 3 cut(s) 253, 1052, 2829
AcsI RAATTY 8 cut(s) 202, 587, 980, 1431, 1525, 1768, 2474, 2741
AcuI CTGAAG 6 cut(s) 444, 660, 846, 2073, 2739, 2753
AfaI GTAC 8 cut(s) 251, 645, 1867, 2087, 2367, 2469, 2819, 2910
AfiI CCNNNNNNNGG 6 cut(s) 725, 1069, 1124, 2161, 2283, 2295
AflIII ACRYGT 1 cut(s) 1743
AjnI CCWGG 3 cut(s) 543, 1012, 2195
AjuI GAANNNNNNNTTGG 4 cut(s) 417, 449, 1838, 1870
AleI CACNNNNGTG 1 cut(s) 1960
Alw21I GWGCWC 4 cut(s) 495, 1504, 1967, 2841
Alw26I GTCTC 7 cut(s) 10, 254, 301, 1189, 1727, 2168, 2387
Alw44I GTGCAC 2 cut(s) 1500, 1963
AlwI GGATC 6 cut(s) 231, 710, 723, 1122, 1135, 2429
AlwNI CAGNNNCTG 3 cut(s) 117, 1664, 1736
ApaI GGGCCC 2 cut(s) 451, 2203
ApaLI GTGCAC 2 cut(s) 1500, 1963
ApoI RAATTY 8 cut(s) 202, 587, 980, 1431, 1525, 1768, 2474, 2741
Asp700I GAANNNNTTC 1 cut(s) 1368
Asp718I GGTACC 2 cut(s) 1865, 2467
AspA2I CCTAGG 1 cut(s) 1870
AspLEI GCGC 1 cut(s) 3093
AsuC2I CCSGG 1 cut(s) 1628
AsuHPI GGTGA 4 cut(s) 722, 1028, 1319, 2123
AvaII GGWCC 6 cut(s) 397, 680, 1387, 1936, 2044, 2286
AvrII CCTAGG 1 cut(s) 1870
AxyI CCTNAGG 1 cut(s) 2262
BaeGI GKGCMC 4 cut(s) 451, 1504, 1967, 2203
BaeI ACNNNNGTAYC 4 cut(s) 1678, 1711, 2695, 2728
BamHI GGATCC 2 cut(s) 715, 1127
BanI GGYRCC 4 cut(s) 317, 1865, 1874, 2467
BanII GRGCYC 3 cut(s) 451, 2014, 2203
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 4 cut(s) 495, 1504, 1967, 2841
BccI CCATC 8 cut(s) 701, 1172, 1184, 1531, 1888, 2458, 2549, 2955
BceAI ACGGC 3 cut(s) 240, 268, 871
BcgI CGANNNNNNTGC 4 cut(s) 1121, 1155, 2534, 2568
BciT130I CCWGG 3 cut(s) 545, 1014, 2197
BciVI GTATCC 1 cut(s) 2723
BclI TGATCA 2 cut(s) 1210, 1822
BcnI CCSGG 1 cut(s) 1628
BcoDI GTCTC 7 cut(s) 10, 254, 301, 1189, 1727, 2168, 2387
BfmI CTRYAG 4 cut(s) 549, 1454, 1712, 2707
BfuI GTATCC 1 cut(s) 2723
BglI GCCNNNNNGGC 1 cut(s) 657
BglII AGATCT 1 cut(s) 3219
BlnI CCTAGG 1 cut(s) 1870
Bme1390I CCNGG 4 cut(s) 545, 1014, 1628, 2197
Bme18I GGWCC 6 cut(s) 397, 680, 1387, 1936, 2044, 2286
BmrFI CCNGG 4 cut(s) 545, 1014, 1628, 2197
BoxI GACNNNNGTC 1 cut(s) 678
BpiI GAAGAC 1 cut(s) 432
BpmI CTGGAG 1 cut(s) 1487
Bpu10I CCTNAGC 1 cut(s) 2118
BpuEI CTTGAG 3 cut(s) 812, 1220, 2057
BpuMI CCSGG 1 cut(s) 1628
Bsa29I ATCGAT 1 cut(s) 2544
BsaBI GATNNNNATC 1 cut(s) 222
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 6 cut(s) 725, 1069, 1124, 2161, 2283, 2295
Bse118I RCCGGY 4 cut(s) 234, 347, 2533, 2750
Bse1I ACTGG 7 cut(s) 133, 1339, 2277, 2331, 2373, 2800, 2906
Bse21I CCTNAGG 1 cut(s) 2262
Bse3DI GCAATG 4 cut(s) 754, 1278, 2103, 2403
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 3 cut(s) 545, 1014, 2197
BseCI ATCGAT 1 cut(s) 2544
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 6 cut(s) 725, 1069, 1124, 2161, 2283, 2295
BseMI GCAATG 4 cut(s) 754, 1278, 2103, 2403
BseMII CTCAG 9 cut(s) 527, 675, 2010, 2132, 2253, 2349, 2362, 2382, 3230
BseNI ACTGG 7 cut(s) 133, 1339, 2277, 2331, 2373, 2800, 2906
BseRI GAGGAG 1 cut(s) 1217
BseSI GKGCMC 4 cut(s) 451, 1504, 1967, 2203
BseX3I CGGCCG 1 cut(s) 253
BseYI CCCAGC 1 cut(s) 2047
BsgI GTGCAG 2 cut(s) 1049, 1662
Bsh1285I CGRYCG 1 cut(s) 256
BshNI GGYRCC 4 cut(s) 317, 1865, 1874, 2467
BshVI ATCGAT 1 cut(s) 2544
BsiEI CGRYCG 1 cut(s) 256
BsiHKAI GWGCWC 4 cut(s) 495, 1504, 1967, 2841
BsiSI CCGG 6 cut(s) 235, 348, 1627, 2534, 2751, 2832
BslFI GGGAC 5 cut(s) 410, 1949, 2030, 2299, 2969
BslI CCNNNNNNNGG 6 cut(s) 725, 1069, 1124, 2161, 2283, 2295
BsmAI GTCTC 7 cut(s) 10, 254, 301, 1189, 1727, 2168, 2387
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 5 cut(s) 410, 1949, 2030, 2299, 2969
BsmI GAATGC 4 cut(s) 883, 1810, 2110, 2156
Bsp120I GGGCCC 2 cut(s) 447, 2199
Bsp1286I GDGCHC 7 cut(s) 451, 495, 1504, 1967, 2014, 2203, 2841
Bsp1407I TGTACA 1 cut(s) 2817
Bsp19I CCATGG 3 cut(s) 832, 1263, 2895
BspACI CCGC 4 cut(s) 27, 176, 271, 3137
BspCNI CTCAG 9 cut(s) 526, 676, 2011, 2131, 2254, 2348, 2361, 2383, 3229
BspDI ATCGAT 1 cut(s) 2544
BspMAI CTGCAG 2 cut(s) 1458, 1716
BspPI GGATC 6 cut(s) 231, 710, 723, 1122, 1135, 2429
BspQI GCTCTTC 1 cut(s) 2886
BspT107I GGYRCC 4 cut(s) 317, 1865, 1874, 2467
BsrDI GCAATG 4 cut(s) 754, 1278, 2103, 2403
BsrFI RCCGGY 4 cut(s) 234, 347, 2533, 2750
BsrGI TGTACA 1 cut(s) 2817
BsrI ACTGG 7 cut(s) 133, 1339, 2277, 2331, 2373, 2800, 2906
BssAI RCCGGY 4 cut(s) 234, 347, 2533, 2750
BssT1I CCWWGG 7 cut(s) 313, 832, 969, 1263, 1383, 1870, 2895
Bst2UI CCWGG 3 cut(s) 545, 1014, 2197
Bst4CI ACNGT 9 cut(s) 158, 694, 1415, 1489, 1660, 1691, 1969, 2234, 2950
Bst6I CTCTTC 3 cut(s) 1592, 1628, 2886
BstAPI GCANNNNNTGC 2 cut(s) 117, 1241
BstAUI TGTACA 1 cut(s) 2817
BstC8I GCNNGC 6 cut(s) 236, 243, 1647, 1726, 1953, 3181
BstDSI CCRYGG 3 cut(s) 832, 1263, 2895
BstENI CCTNNNNNAGG 1 cut(s) 2159
BstHHI GCGC 1 cut(s) 3093
BstMAI GTCTC 7 cut(s) 10, 254, 301, 1189, 1727, 2168, 2387
BstMCI CGRYCG 1 cut(s) 256
BstNI CCWGG 3 cut(s) 545, 1014, 2197
BstNSI RCATGY 1 cut(s) 3073
BstPAI GACNNNNGTC 1 cut(s) 678
BstSCI CCNGG 4 cut(s) 543, 1012, 1626, 2195
BstSFI CTRYAG 4 cut(s) 549, 1454, 1712, 2707
BstSLI GKGCMC 4 cut(s) 451, 1504, 1967, 2203
BstV2I GAAGAC 1 cut(s) 432
BstX2I RGATCY 4 cut(s) 223, 715, 1127, 3219
BstYI RGATCY 4 cut(s) 223, 715, 1127, 3219
BstZI CGGCCG 1 cut(s) 253
Bsu15I ATCGAT 1 cut(s) 2544
Bsu36I CCTNAGG 1 cut(s) 2262
BsuI GTATCC 1 cut(s) 2723
BsuTUI ATCGAT 1 cut(s) 2544
BtgI CCRYGG 3 cut(s) 832, 1263, 2895
BtgZI GCGATG 2 cut(s) 1843, 2731
BtsI GCAGTG 6 cut(s) 118, 667, 1668, 1709, 1996, 2781
Cac8I GCNNGC 6 cut(s) 236, 243, 1647, 1726, 1953, 3181
CaiI CAGNNNCTG 3 cut(s) 117, 1664, 1736
CfoI GCGC 1 cut(s) 3093
Cfr10I RCCGGY 4 cut(s) 234, 347, 2533, 2750
ClaI ATCGAT 1 cut(s) 2544
CseI GACGC 1 cut(s) 157
CsiI ACCWGGT 1 cut(s) 1012
Csp6I GTAC 8 cut(s) 250, 644, 1866, 2086, 2366, 2468, 2818, 2909
CspCI CAANNNNNGTGG 2 cut(s) 2770, 2805
CviAII CATG 9 cut(s) 184, 371, 771, 833, 1264, 2007, 2896, 3070, 3094
CviQI GTAC 8 cut(s) 250, 644, 1866, 2086, 2366, 2468, 2818, 2909
EaeI YGGCCR 3 cut(s) 253, 1052, 2829
EagI CGGCCG 1 cut(s) 253
Eam1104I CTCTTC 3 cut(s) 1592, 1628, 2886
EarI CTCTTC 3 cut(s) 1592, 1628, 2886
EciI GGCGGA 2 cut(s) 42, 3152
EclXI CGGCCG 1 cut(s) 253
Eco130I CCWWGG 7 cut(s) 313, 832, 969, 1263, 1383, 1870, 2895
Eco147I AGGCCT 2 cut(s) 312, 1310
Eco24I GRGCYC 3 cut(s) 451, 2014, 2203
Eco32I GATATC 2 cut(s) 565, 768
Eco47I GGWCC 6 cut(s) 397, 680, 1387, 1936, 2044, 2286
Eco52I CGGCCG 1 cut(s) 253
Eco57I CTGAAG 6 cut(s) 444, 660, 846, 2073, 2739, 2753
Eco81I CCTNAGG 1 cut(s) 2262
EcoNI CCTNNNNNAGG 1 cut(s) 2159
EcoO109I RGGNCCY 6 cut(s) 397, 448, 649, 1936, 2044, 2286
EcoRI GAATTC 2 cut(s) 202, 587
EcoRII CCWGG 3 cut(s) 543, 1012, 2195
EcoRV GATATC 2 cut(s) 565, 768
EcoT14I CCWWGG 7 cut(s) 313, 832, 969, 1263, 1383, 1870, 2895
EcoT38I GRGCYC 3 cut(s) 451, 2014, 2203
ErhI CCWWGG 7 cut(s) 313, 832, 969, 1263, 1383, 1870, 2895
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 9 cut(s) 187, 374, 774, 836, 1267, 2010, 2899, 3073, 3097
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 5 cut(s) 410, 1949, 2030, 2299, 2969
FatI CATG 9 cut(s) 183, 370, 770, 832, 1263, 2006, 2895, 3069, 3093
FauNDI CATATG 2 cut(s) 2664, 3105
FbaI TGATCA 2 cut(s) 1210, 1822
FblI GTMKAC 3 cut(s) 1612, 2429, 2724
FriOI GRGCYC 3 cut(s) 451, 2014, 2203
GlaI GCGC 1 cut(s) 3092
GsaI CCCAGC 1 cut(s) 2051
GsuI CTGGAG 1 cut(s) 1487
HapII CCGG 6 cut(s) 235, 348, 1627, 2534, 2751, 2832
HgaI GACGC 1 cut(s) 157
HhaI GCGC 1 cut(s) 3093
Hin1II CATG 9 cut(s) 187, 374, 774, 836, 1267, 2010, 2899, 3073, 3097
Hin6I GCGC 1 cut(s) 3091
HinP1I GCGC 1 cut(s) 3091
HincII GTYRAC 3 cut(s) 2247, 2455, 2725
HindII GTYRAC 3 cut(s) 2247, 2455, 2725
HindIII AAGCTT 1 cut(s) 86
HpaI GTTAAC 2 cut(s) 2247, 2455
HpaII CCGG 6 cut(s) 235, 348, 1627, 2534, 2751, 2832
HphI GGTGA 4 cut(s) 722, 1028, 1319, 2123
Hpy188I TCNGA 9 cut(s) 538, 1029, 1102, 1132, 1215, 1374, 2020, 3156, 3219
Hpy99I CGWCG 1 cut(s) 101
HpyCH4III ACNGT 9 cut(s) 158, 694, 1415, 1489, 1660, 1691, 1969, 2234, 2950
HpyCH4IV ACGT 3 cut(s) 1111, 1743, 2460
HpySE526I ACGT 3 cut(s) 1111, 1743, 2460
Hsp92II CATG 9 cut(s) 187, 374, 774, 836, 1267, 2010, 2899, 3073, 3097
HspAI GCGC 1 cut(s) 3091
KflI GGGWCCC 2 cut(s) 1936, 2286
KpnI GGTACC 2 cut(s) 1869, 2471
KroI GCCGGC 1 cut(s) 234
KroNI GCCGGC 1 cut(s) 236
Ksp22I TGATCA 2 cut(s) 1210, 1822
KspAI GTTAAC 2 cut(s) 2247, 2455
LguI GCTCTTC 1 cut(s) 2886
LmnI GCTCC 8 cut(s) 1445, 1925, 2308, 2596, 2607, 2812, 3000, 3139
MabI ACCWGGT 1 cut(s) 1012
MaeII ACGT 3 cut(s) 1111, 1743, 2460
MaeIII GTNAC 8 cut(s) 113, 131, 361, 671, 949, 1162, 1226, 1601
MfeI CAATTG 3 cut(s) 582, 900, 2769
MflI RGATCY 4 cut(s) 223, 715, 1127, 3219
MhlI GDGCHC 7 cut(s) 451, 495, 1504, 1967, 2014, 2203, 2841
MmeI TCCRAC 4 cut(s) 309, 1270, 1397, 2253
MroNI GCCGGC 1 cut(s) 234
MroXI GAANNNNTTC 1 cut(s) 1368
MslI CAYNNNNRTG 5 cut(s) 1507, 1892, 1960, 2667, 3165
MspA1I CMGCKG 2 cut(s) 517, 2051
MspI CCGG 6 cut(s) 235, 348, 1627, 2534, 2751, 2832
MspR9I CCNGG 4 cut(s) 545, 1014, 1628, 2197
MunI CAATTG 3 cut(s) 582, 900, 2769
Mva1269I GAATGC 4 cut(s) 883, 1810, 2110, 2156
MvaI CCWGG 3 cut(s) 545, 1014, 2197
NaeI GCCGGC 1 cut(s) 236
NciI CCSGG 1 cut(s) 1628
NcoI CCATGG 3 cut(s) 832, 1263, 2895
NdeI CATATG 2 cut(s) 2664, 3105
NgoMIV GCCGGC 1 cut(s) 234
NlaIII CATG 9 cut(s) 187, 374, 774, 836, 1267, 2010, 2899, 3073, 3097
NmeAIII GCCGAG 3 cut(s) 346, 888, 1163
NmuCI GTSAC 4 cut(s) 113, 361, 671, 1226
NspI RCATGY 1 cut(s) 3073
OliI CACNNNNGTG 1 cut(s) 1960
PceI AGGCCT 2 cut(s) 312, 1310
PciSI GCTCTTC 1 cut(s) 2886
PctI GAATGC 4 cut(s) 883, 1810, 2110, 2156
PdiI GCCGGC 1 cut(s) 236
PdmI GAANNNNTTC 1 cut(s) 1368
PfoI TCCNGGA 1 cut(s) 1626
PpuMI RGGWCCY 4 cut(s) 397, 1936, 2044, 2286
PshAI GACNNNNGTC 1 cut(s) 678
PsiI TTATAA 2 cut(s) 1685, 2969
Psp5II RGGWCCY 4 cut(s) 397, 1936, 2044, 2286
Psp6I CCWGG 3 cut(s) 543, 1012, 2195
PspFI CCCAGC 1 cut(s) 2047
PspGI CCWGG 3 cut(s) 543, 1012, 2195
PspOMI GGGCCC 2 cut(s) 447, 2199
PspPPI RGGWCCY 4 cut(s) 397, 1936, 2044, 2286
PstI CTGCAG 2 cut(s) 1458, 1716
PstNI CAGNNNCTG 3 cut(s) 117, 1664, 1736
PsuI RGATCY 4 cut(s) 223, 715, 1127, 3219
PvuII CAGCTG 2 cut(s) 517, 2051
RsaI GTAC 8 cut(s) 251, 645, 1867, 2087, 2367, 2469, 2819, 2910
RsaNI GTAC 8 cut(s) 250, 644, 1866, 2086, 2366, 2468, 2818, 2909
RseI CAYNNNNRTG 5 cut(s) 1507, 1892, 1960, 2667, 3165
SalI GTCGAC 1 cut(s) 2723
SapI GCTCTTC 1 cut(s) 2886
ScrFI CCNGG 4 cut(s) 545, 1014, 1628, 2197
SduI GDGCHC 7 cut(s) 451, 495, 1504, 1967, 2014, 2203, 2841
SexAI ACCWGGT 1 cut(s) 1012
SfcI CTRYAG 4 cut(s) 549, 1454, 1712, 2707
SinI GGWCC 6 cut(s) 397, 680, 1387, 1936, 2044, 2286
SmiMI CAYNNNNRTG 5 cut(s) 1507, 1892, 1960, 2667, 3165
SmlI CTYRAG 3 cut(s) 791, 1199, 2036
SmoI CTYRAG 3 cut(s) 791, 1199, 2036
SseBI AGGCCT 2 cut(s) 312, 1310
SsiI CCGC 4 cut(s) 27, 176, 271, 3137
SspI AATATT 1 cut(s) 1702
StuI AGGCCT 2 cut(s) 312, 1310
StyD4I CCNGG 4 cut(s) 543, 1012, 1626, 2195
StyI CCWWGG 7 cut(s) 313, 832, 969, 1263, 1383, 1870, 2895
TaaI ACNGT 9 cut(s) 158, 694, 1415, 1489, 1660, 1691, 1969, 2234, 2950
TaiI ACGT 3 cut(s) 1114, 1746, 2463
TaqI TCGA 5 cut(s) 591, 777, 1353, 2544, 2724
TaqII GACCGA 1 cut(s) 857
TatI WGTACW 3 cut(s) 2085, 2365, 2817
TauI GCSGC 2 cut(s) 178, 274
TseFI GTSAC 4 cut(s) 113, 361, 671, 1226
Tsp45I GTSAC 4 cut(s) 113, 361, 671, 1226
TspDTI ATGAA 9 cut(s) 390, 882, 1141, 1961, 1965, 2114, 2591, 2636, 2734
TspGWI ACGGA 1 cut(s) 870
VneI GTGCAC 2 cut(s) 1500, 1963
VpaK11BI GGWCC 6 cut(s) 397, 680, 1387, 1936, 2044, 2286
XagI CCTNNNNNAGG 1 cut(s) 2159
XapI RAATTY 8 cut(s) 202, 587, 980, 1431, 1525, 1768, 2474, 2741
XbaI TCTAGA 1 cut(s) 1792
XceI RCATGY 1 cut(s) 3073
XmaJI CCTAGG 1 cut(s) 1870
XmiI GTMKAC 3 cut(s) 1612, 2429, 2724
XmnI GAANNNNTTC 1 cut(s) 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.