AT2G34350

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001991
Physical Location & Seq
Forward (+)
14493308 .. 14495831
2524 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G34350.2

Sequence Viewer

Length: 1578 bp
ATGGAGTTCGCAAACACGAAATGGGTGGCTGCAGCGGCGAGCATATGGATACAAAGCTTCAGCGGAGCAAGCTACACTTTTGGAATTTATTCTTCTGTTCTTAAATCATCTCAATCTTATGACCAATCTACTCTTGACACCGTCTCTGTCTACAAAGACATCGGAGCAAACGTAGGCATCCTCTCCGGCCTCTTCTATACCGCGGTTGCAAGCCGAAAAAGCGGTAATGGTGGCTTCTTTAGTGGGCCATGGTTGGTTATATTTGTGGGGCTACTCCAATGGTTCGTCGGATATGGATTCATATGGATGGCGACGTCAGGAGTGATCCCACGGCCTCCCGTGGCTATGATGTGTTTGTTCATGTTCTTCGCTGGTCATTGTCAGCCTTTCTTTAATACCGCGATTGTGGTCACCGCCGTTCGTAACTTCTCCGACTATGGTGGGACCGCCGTTGGCATTATGAAGGGTTATCTTGGACTAAGTGGAGCAATTCTGGTTCAAATGTACCACATCTTCTGTGGAGGTGACCCAAGGAATTACATTCTTCTCTTGGCCGTGGTACCATCACTTCTCATCTTGACGTTGATGCCCTTCGTTAGAACGTACGACACAGTCATCGCAGGCGACAAGAAACACTTGAACGGTCTCTCTGCCATTTCTTTGATCATCGTCACCTATCTTATGGTCGTCATATTGGTCGAAAACATTATCGGAATGTCAATGCCTATGAAGATATGTTCCTTTACCTTTCTACTTCTCTTGCTCGCCTCTCCTCTCTTAGTGGCGGTTAGAGCACAACGTGAAGAGGAACATAGATTCTTGTCTTTGGATTTTCCTGTTACCGAGAGAACCACATTGCTTGACTCTCCTAAGCTCAATTCTTCTTCCGATGTTAAAGATGTCATGACCAATGACATGAACGTTCTCGAAGCAATATGCACAACGAATTTCTGGCTTCTATTTGTAGCAATGATATGTGGAATGGGTTCAGGACTTGCGACAATAAACAACATCAGACAGATGGGCGAGTCACTTCGCTACTCTACGGTTCAACTCAATTCTCTAGTGTCTCTGTGGAGCATATGGAACTTTCTAGGCCGGTTTGGGTCGGGTTACATCTCAGACACCTATTTACACAGTCATGGCTGGCCAAGACCGGTATTCATGGCCATAACCCTTGGCCTTATGGCTATAGGTCACATTGTCATGGCCTCCGGTTTACTAGGAAGCCTATATATCGGATCCTTGTTGGTCGGTTTAGCGTACGGCTCCCAATGGTCACTCATGCCAACAATAACCTCTGAAATATTCGGGGTTCTTCATATGGGAACTATATTCTATACAATTTCGATAGCTAGTCCGGTTGGTTCGTACTTTTTCTCAGTGAAGGTAATAGGCTACTTGTATGACAAGGTAGCTTCTGAAGATGATCATTCTTGTTATGGAAATCACTGTTTTAGGACTTCGTTTTTGATAATGGCGGCCATGGCTCTGCTTGGTTCTCTTGTCGCTCTTGTGTTGCTCCTCCGCACAAAGAAGTTTTATGCGACGCTTGTGGCTAAGAGGATCTTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

525

Amino Acids

57.57

Weight (kDa)

8.76

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 7 - 262 1e-60 Nodulin-like
NFD4_C PF23262 312 - 516 2.4e-18 NFD4, C-terminal domain
MFS_1 PF07690 319 - 513 3.6e-11 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 317
Acc65I GGTACC 1 cut(s) 559
AccB1I GGYRCC 1 cut(s) 559
AccI GTMKAC 1 cut(s) 150
AccII CGCG 2 cut(s) 203, 401
AclI AACGTT 1 cut(s) 921
AclWI GGATC 4 cut(s) 319, 1236, 1249, 1574
AcoI YGGCCR 4 cut(s) 552, 1148, 1167, 1482
AcsI RAATTY 2 cut(s) 84, 946
AcuI CTGAAG 2 cut(s) 43, 1443
AcyI GRCGYC 1 cut(s) 314
AdeI CACNNNGTG 1 cut(s) 800
AfaI GTAC 5 cut(s) 506, 561, 605, 1265, 1373
AgeI ACCGGT 1 cut(s) 1156
AgsI TTSAA 4 cut(s) 500, 640, 1052, 1573
AluBI AGCT 5 cut(s) 57, 72, 874, 1355, 1418
AluI AGCT 5 cut(s) 57, 72, 874, 1355, 1418
Alw21I GWGCWC 1 cut(s) 796
Alw26I GTCTC 3 cut(s) 148, 650, 1074
AlwI GGATC 4 cut(s) 319, 1236, 1249, 1574
ApeKI GCWGC 2 cut(s) 29, 32
ApoI RAATTY 2 cut(s) 84, 946
ArsI GACNNNNNNTTYG 2 cut(s) 1453, 1485
AsiGI ACCGGT 1 cut(s) 1156
Asp700I GAANNNNTTC 2 cut(s) 88, 985
Asp718I GGTACC 1 cut(s) 559
AspS9I GGNCC 2 cut(s) 245, 444
AsuHPI GGTGA 3 cut(s) 403, 536, 664
AvaII GGWCC 1 cut(s) 444
BalI TGGCCA 2 cut(s) 1150, 1169
BamHI GGATCC 1 cut(s) 1241
BanI GGYRCC 1 cut(s) 559
BarI GAAGNNNNNNTAC 4 cut(s) 497, 529, 552, 584
Bbv12I GWGCWC 1 cut(s) 796
BbvI GCAGC 2 cut(s) 16, 44
BccI CCATC 3 cut(s) 301, 571, 1015
BceAI ACGGC 5 cut(s) 347, 401, 434, 539, 1282
BciVI GTATCC 1 cut(s) 42
BclI TGATCA 2 cut(s) 663, 1429
BcoDI GTCTC 3 cut(s) 148, 650, 1074
BfaI CTAG 4 cut(s) 1064, 1094, 1223, 1356
BfmI CTRYAG 2 cut(s) 30, 1191
BfuI GTATCC 1 cut(s) 42
BisI GCNGC 4 cut(s) 30, 33, 36, 1482
BlsI GCNGC 4 cut(s) 31, 34, 37, 1483
Bme18I GGWCC 1 cut(s) 444
BmgT120I GGNCC 2 cut(s) 245, 444
BmiI GGNNCC 4 cut(s) 445, 561, 1243, 1270
BmsI GCATC 2 cut(s) 186, 576
Bpu10I CCTNAGC 1 cut(s) 870
BsaHI GRCGYC 1 cut(s) 314
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 8 cut(s) 201, 248, 329, 339, 530, 555, 1177, 1485
BsaWI WCCGGW 3 cut(s) 1156, 1214, 1360
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bse118I RCCGGY 2 cut(s) 1098, 1156
Bse3DI GCAATG 2 cut(s) 854, 975
BseDI CCNNGG 8 cut(s) 201, 248, 329, 339, 530, 555, 1177, 1485
BseGI GGATG 2 cut(s) 177, 312
BseMI GCAATG 2 cut(s) 854, 975
BseMII CTCAG 2 cut(s) 1134, 1395
BseRI GAGGAG 2 cut(s) 762, 1514
BseXI GCAGC 2 cut(s) 16, 44
Bsh1236I CGCG 2 cut(s) 203, 401
BshNI GGYRCC 1 cut(s) 559
BshTI ACCGGT 1 cut(s) 1156
BsiHKAI GWGCWC 1 cut(s) 796
BsiSI CCGG 5 cut(s) 186, 1099, 1157, 1215, 1361
BsiWI CGTACG 2 cut(s) 603, 1263
BslFI GGGAC 1 cut(s) 457
BsmAI GTCTC 3 cut(s) 148, 650, 1074
BsmBI CGTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 457
Bso31I GGTCTC 1 cut(s) 650
Bsp1286I GDGCHC 1 cut(s) 796
Bsp143I GATC 5 cut(s) 324, 663, 1241, 1429, 1566
Bsp19I CCATGG 2 cut(s) 248, 1485
BspCNI CTCAG 2 cut(s) 1133, 1394
BspFNI CGCG 2 cut(s) 203, 401
BspHI TCATGA 1 cut(s) 903
BspLI GGNNCC 4 cut(s) 445, 561, 1243, 1270
BspMAI CTGCAG 1 cut(s) 34
BspPI GGATC 4 cut(s) 319, 1236, 1249, 1574
BspT107I GGYRCC 1 cut(s) 559
BspTNI GGTCTC 1 cut(s) 650
BsrDI GCAATG 2 cut(s) 854, 975
BsrFI RCCGGY 2 cut(s) 1098, 1156
BssAI RCCGGY 2 cut(s) 1098, 1156
BssECI CCNNGG 8 cut(s) 201, 248, 329, 339, 530, 555, 1177, 1485
BssMI GATC 5 cut(s) 324, 663, 1241, 1429, 1566
BssNI GRCGYC 1 cut(s) 314
BssT1I CCWWGG 4 cut(s) 248, 530, 1177, 1485
Bst4CI ACNGT 6 cut(s) 142, 613, 644, 1048, 1139, 1454
Bst6I CTCTTC 2 cut(s) 197, 798
BstACI GRCGYC 1 cut(s) 314
BstC8I GCNNGC 6 cut(s) 40, 70, 211, 622, 765, 1148
BstDEI CTNAG 6 cut(s) 479, 778, 870, 1120, 1381, 1560
BstDSI CCRYGG 6 cut(s) 201, 248, 329, 339, 555, 1485
BstEII GGTNACC 2 cut(s) 409, 524
BstF5I GGATG 2 cut(s) 177, 312
BstFNI CGCG 2 cut(s) 203, 401
BstKTI GATC 5 cut(s) 327, 666, 1244, 1432, 1569
BstMAI GTCTC 3 cut(s) 148, 650, 1074
BstMBI GATC 5 cut(s) 324, 663, 1241, 1429, 1566
BstMWI GCNNNNNNNGC 5 cut(s) 35, 69, 219, 791, 1487
BstPI GGTNACC 2 cut(s) 409, 524
BstSFI CTRYAG 2 cut(s) 30, 1191
BstUI CGCG 2 cut(s) 203, 401
BstV1I GCAGC 2 cut(s) 16, 44
BstX2I RGATCY 2 cut(s) 1241, 1566
BstYI RGATCY 2 cut(s) 1241, 1566
BsuI GTATCC 1 cut(s) 42
BtgI CCRYGG 6 cut(s) 201, 248, 329, 339, 555, 1485
BtgZI GCGATG 1 cut(s) 601
BtsCI GGATG 2 cut(s) 177, 312
BtsIMutI CAGTG 2 cut(s) 1389, 1450
Cac8I GCNNGC 6 cut(s) 40, 70, 211, 622, 765, 1148
CciI TCATGA 1 cut(s) 903
Cfr10I RCCGGY 2 cut(s) 1098, 1156
Cfr13I GGNCC 2 cut(s) 245, 444
Cfr42I CCGCGG 1 cut(s) 204
CseI GACGC 1 cut(s) 1558
Csp6I GTAC 5 cut(s) 505, 560, 604, 1264, 1372
CspAI ACCGGT 1 cut(s) 1156
CspCI CAANNNNNGTGG 2 cut(s) 841, 876
CviAII CATG 9 cut(s) 249, 361, 904, 916, 1142, 1165, 1207, 1285, 1486
CviQI GTAC 5 cut(s) 505, 560, 604, 1264, 1372
DdeI CTNAG 6 cut(s) 479, 778, 870, 1120, 1381, 1560
DpnI GATC 5 cut(s) 326, 665, 1243, 1431, 1568
DpnII GATC 5 cut(s) 324, 663, 1241, 1429, 1566
DraIII CACNNNGTG 1 cut(s) 800
EaeI YGGCCR 4 cut(s) 552, 1148, 1167, 1482
Eam1104I CTCTTC 2 cut(s) 197, 798
EarI CTCTTC 2 cut(s) 197, 798
Eco130I CCWWGG 4 cut(s) 248, 530, 1177, 1485
Eco31I GGTCTC 1 cut(s) 650
Eco47I GGWCC 1 cut(s) 444
Eco57I CTGAAG 2 cut(s) 43, 1443
Eco91I GGTNACC 2 cut(s) 409, 524
EcoO65I GGTNACC 2 cut(s) 409, 524
EcoT14I CCWWGG 4 cut(s) 248, 530, 1177, 1485
ErhI CCWWGG 4 cut(s) 248, 530, 1177, 1485
Esp3I CGTCTC 1 cut(s) 148
FaeI CATG 9 cut(s) 252, 364, 907, 919, 1145, 1168, 1210, 1288, 1489
FalI AAGNNNNNCTT 5 cut(s) 61, 93, 620, 652, 1553
FaqI GGGAC 1 cut(s) 457
FatI CATG 9 cut(s) 248, 360, 903, 915, 1141, 1164, 1206, 1284, 1485
FauNDI CATATG 4 cut(s) 44, 302, 1082, 1323
FbaI TGATCA 2 cut(s) 663, 1429
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 4 cut(s) 30, 33, 36, 1482
FokI GGATG 2 cut(s) 164, 319
Fsp4HI GCNGC 4 cut(s) 30, 33, 36, 1482
FspBI CTAG 4 cut(s) 1064, 1094, 1223, 1356
GluI GCNGC 4 cut(s) 30, 33, 36, 1482
HapII CCGG 5 cut(s) 186, 1099, 1157, 1215, 1361
HgaI GACGC 1 cut(s) 1558
Hin1I GRCGYC 1 cut(s) 314
Hin1II CATG 9 cut(s) 252, 364, 907, 919, 1145, 1168, 1210, 1288, 1489
HindIII AAGCTT 1 cut(s) 55
HinfI GANTC 4 cut(s) 297, 816, 863, 1028
HpaII CCGG 5 cut(s) 186, 1099, 1157, 1215, 1361
HphI GGTGA 3 cut(s) 403, 536, 664
Hpy166II GTNNAC 2 cut(s) 151, 1220
Hpy188III TCNNGA 7 cut(s) 134, 318, 577, 904, 926, 990, 1570
Hpy8I GTNNAC 2 cut(s) 151, 1220
Hpy99I CGWCG 3 cut(s) 290, 316, 1552
HpyAV CCTTC 3 cut(s) 457, 601, 1381
HpyCH4III ACNGT 6 cut(s) 142, 613, 644, 1048, 1139, 1454
HpyCH4IV ACGT 6 cut(s) 171, 314, 581, 602, 799, 921
HpyCH4V TGCA 3 cut(s) 32, 209, 939
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 69, 219, 791, 1487
HpyF3I CTNAG 6 cut(s) 479, 778, 870, 1120, 1381, 1560
HpySE526I ACGT 6 cut(s) 171, 314, 581, 602, 799, 921
Hsp92I GRCGYC 1 cut(s) 314
Hsp92II CATG 9 cut(s) 252, 364, 907, 919, 1145, 1168, 1210, 1288, 1489
KpnI GGTACC 1 cut(s) 563
Ksp22I TGATCA 2 cut(s) 663, 1429
KspI CCGCGG 1 cut(s) 204
Kzo9I GATC 5 cut(s) 324, 663, 1241, 1429, 1566
LmnI GCTCC 6 cut(s) 65, 164, 485, 1077, 1274, 1527
Lsp1109I GCAGC 2 cut(s) 16, 44
LweI GCATC 2 cut(s) 186, 576
MaeI CTAG 4 cut(s) 1064, 1094, 1223, 1356
MaeII ACGT 6 cut(s) 171, 314, 581, 602, 799, 921
MaeIII GTNAC 9 cut(s) 409, 422, 524, 670, 838, 1029, 1112, 1196, 1278
MalI GATC 5 cut(s) 326, 665, 1243, 1431, 1568
MboI GATC 5 cut(s) 324, 663, 1241, 1429, 1566
MflI RGATCY 2 cut(s) 1241, 1566
MhlI GDGCHC 1 cut(s) 796
MlsI TGGCCA 2 cut(s) 1150, 1169
MluCI AATT 7 cut(s) 84, 489, 535, 877, 946, 1057, 1344
MluNI TGGCCA 2 cut(s) 1150, 1169
MlyI GAGTC 2 cut(s) 857, 1037
MmeI TCCRAC 2 cut(s) 268, 456
Mox20I TGGCCA 2 cut(s) 1150, 1169
MroXI GAANNNNTTC 2 cut(s) 88, 985
MscI TGGCCA 2 cut(s) 1150, 1169
MseI TTAA 3 cut(s) 102, 393, 894
MslI CAYNNNNRTG 3 cut(s) 305, 1140, 1205
Msp20I TGGCCA 2 cut(s) 1150, 1169
MspA1I CMGCKG 3 cut(s) 35, 63, 203
MspI CCGG 5 cut(s) 186, 1099, 1157, 1215, 1361
MvnI CGCG 2 cut(s) 203, 401
MwoI GCNNNNNNNGC 5 cut(s) 35, 69, 219, 791, 1487
NcoI CCATGG 2 cut(s) 248, 1485
NdeI CATATG 4 cut(s) 44, 302, 1082, 1323
NdeII GATC 5 cut(s) 324, 663, 1241, 1429, 1566
NlaIII CATG 9 cut(s) 252, 364, 907, 919, 1145, 1168, 1210, 1288, 1489
NlaIV GGNNCC 4 cut(s) 445, 561, 1243, 1270
NmuCI GTSAC 6 cut(s) 409, 524, 670, 1029, 1196, 1278
PagI TCATGA 1 cut(s) 903
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PdmI GAANNNNTTC 2 cut(s) 88, 985
PfeI GAWTC 2 cut(s) 297, 816
Pfl23II CGTACG 2 cut(s) 603, 1263
PflFI GACNNNGTC 2 cut(s) 140, 611
PinAI ACCGGT 1 cut(s) 1156
PkrI GCNGC 4 cut(s) 31, 34, 37, 1483
PleI GAGTC 2 cut(s) 857, 1036
PpsI GAGTC 2 cut(s) 857, 1036
Psp1406I AACGTT 1 cut(s) 921
PspEI GGTNACC 2 cut(s) 409, 524
PspLI CGTACG 2 cut(s) 603, 1263
PspN4I GGNNCC 4 cut(s) 445, 561, 1243, 1270
PspPI GGNCC 2 cut(s) 245, 444
PstI CTGCAG 1 cut(s) 34
PsuI RGATCY 2 cut(s) 1241, 1566
PsyI GACNNNGTC 2 cut(s) 140, 611
RsaI GTAC 5 cut(s) 506, 561, 605, 1265, 1373
RsaNI GTAC 5 cut(s) 505, 560, 604, 1264, 1372
RseI CAYNNNNRTG 3 cut(s) 305, 1140, 1205
SacII CCGCGG 1 cut(s) 204
SaqAI TTAA 3 cut(s) 102, 393, 894
SatI GCNGC 4 cut(s) 30, 33, 36, 1482
Sau3AI GATC 5 cut(s) 324, 663, 1241, 1429, 1566
Sau96I GGNCC 2 cut(s) 245, 444
SchI GAGTC 2 cut(s) 857, 1037
SduI GDGCHC 1 cut(s) 796
SfaNI GCATC 2 cut(s) 186, 576
SfcI CTRYAG 2 cut(s) 30, 1191
Sfr303I CCGCGG 1 cut(s) 204
SgrBI CCGCGG 1 cut(s) 204
SinI GGWCC 1 cut(s) 444
SmiMI CAYNNNNRTG 3 cut(s) 305, 1140, 1205
Sse9I AATT 7 cut(s) 84, 489, 535, 877, 946, 1057, 1344
SspI AATATT 1 cut(s) 1308
SspMI CTAG 4 cut(s) 1064, 1094, 1223, 1356
StyI CCWWGG 4 cut(s) 248, 530, 1177, 1485
TaaI ACNGT 6 cut(s) 142, 613, 644, 1048, 1139, 1454
TaiI ACGT 6 cut(s) 174, 317, 584, 605, 802, 924
TaqI TCGA 3 cut(s) 699, 927, 1349
TasI AATT 7 cut(s) 84, 489, 535, 877, 946, 1057, 1344
TauI GCSGC 2 cut(s) 38, 1484
TfiI GAWTC 2 cut(s) 297, 816
Tru1I TTAA 3 cut(s) 102, 393, 894
Tru9I TTAA 3 cut(s) 102, 393, 894
TscAI CASTG 2 cut(s) 1389, 1457
TseFI GTSAC 6 cut(s) 409, 524, 670, 1029, 1196, 1278
TseI GCWGC 2 cut(s) 29, 32
Tsp45I GTSAC 6 cut(s) 409, 524, 670, 1029, 1196, 1278
TspDTI ATGAA 7 cut(s) 289, 349, 476, 743, 932, 1153, 1310
TspRI CASTG 2 cut(s) 1389, 1457
Tth111I GACNNNGTC 2 cut(s) 140, 611
VpaK11BI GGWCC 1 cut(s) 444
XapI RAATTY 2 cut(s) 84, 946
XcmI CCANNNNNNNNNTGG 1 cut(s) 515
XmiI GTMKAC 1 cut(s) 150
XmnI GAANNNNTTC 2 cut(s) 88, 985
XspI CTAG 4 cut(s) 1064, 1094, 1223, 1356
ZraI GACGTC 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.