MD00G1132000.v1.1

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0001991
Physical Location & Seq
Forward (+)
28322619 .. 28324933
2315 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1132000.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGCAGCGATGGAGATGCTGAGCAGCAGCAAGTGGAAAGCCACGGCGGCAAGTATATGGATTCAGTGCGCTGGAGGCGCTTCCTACGCCTTCGGCATATATTCCTCTGCGCTCAAGTCGAGCCAGGGCTACGATCAATCGACGCTCGACATGGTGTCCGTCTTCAAGGACATAGGTGCCAACGCCGGCGTCCTCTCGGGGATTCTGTACTCCGTGGTCGCATTCAACGGTCGCCGGATTGGTGGGCCGTGGGTGGTGCTCACTCCGCAGGTGGCGGTTGTGTGTTTGTTCATGTTCTTGGCTGCCCACTCTCAGACTTTCTTCAGCACCGCCAACGTGGTCACCGGGGTCAACAATTTCTCTGATTACAGTGGGACCATCGTTGGCATACTGAAGGGATTTCTTGGTCTGAGTTCAGCACTTCTTGTCCAAGTCTATGACACATTATTCTGCAAAGGCAACCCCAGCGCTTTCCTTCTCCTGCTGGCCTTGTTTCCCACGTCTGTCACCATCCTCTGCATGCCTTTGGTCAGAATCTATGAAGCTGGTGGAGCTGCTTCTGACAAAAAGTACTTAAAACATCTTCTCTGCTTTGCTTCTCATCACTTCTGCTTATCTCATGGTCTTAATCATATTCCAAAACATCCTCACTTTCCCATCATGGGCGAGCATTCTCACATTCACAGCACTTCTTCTTGCTTTAATCGCCTCGCCCCTCGGAATCGCATTCAAAGCCCAAAACTTGCAACCCAAGAAACTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

255

Amino Acids

27.35

Weight (kDa)

9.1

Isoelectric Point (pI)

31.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 11 - 194 1e-39 Nodulin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 259
Acc36I ACCTGC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 176
AciI CCGC 4 cut(s) 47, 266, 275, 330
AcuI CTGAAG 2 cut(s) 307, 413
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 2 cut(s) 209, 572
AfeI AGCGCT 1 cut(s) 469
AfiI CCNNNNNNNGG 1 cut(s) 662
AgsI TTSAA 3 cut(s) 166, 226, 731
AhdI GACNNNNNGTC 1 cut(s) 154
AjiI CACGTC 1 cut(s) 501
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 2 cut(s) 545, 554
AluI AGCT 2 cut(s) 545, 554
Alw21I GWGCWC 1 cut(s) 261
Ama87I CYCGRG 1 cut(s) 196
Aor51HI AGCGCT 1 cut(s) 469
AoxI GGCC 2 cut(s) 245, 486
ApeKI GCWGC 5 cut(s) 5, 24, 27, 302, 554
AspLEI GCGC 4 cut(s) 71, 80, 112, 470
AspS9I GGNCC 2 cut(s) 245, 375
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 2 cut(s) 334, 499
AvaI CYCGRG 1 cut(s) 196
AvaII GGWCC 1 cut(s) 375
BanI GGYRCC 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 5 cut(s) 17, 36, 39, 289, 541
BccI CCATC 4 cut(s) 4, 386, 518, 665
BceAI ACGGC 2 cut(s) 60, 232
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 125
BcnI CCSGG 1 cut(s) 346
BfoI RGCGCY 2 cut(s) 81, 471
BfuAI ACCTGC 1 cut(s) 259
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 6 cut(s) 6, 25, 28, 48, 303, 555
BlpI GCTNAGC 1 cut(s) 20
BlsI GCNGC 6 cut(s) 7, 26, 29, 49, 304, 556
BmcAI AGTACT 1 cut(s) 572
Bme1390I CCNGG 2 cut(s) 125, 346
Bme18I GGWCC 1 cut(s) 375
BmeRI GACNNNNNGTC 1 cut(s) 154
BmeT110I CYCGRG 1 cut(s) 196
BmgBI CACGTC 1 cut(s) 501
BmgT120I GGNCC 2 cut(s) 245, 375
BmiI GGNNCC 2 cut(s) 178, 376
BmrFI CCNGG 2 cut(s) 125, 346
BmsI GCATC 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 98
BpuMI CCSGG 1 cut(s) 346
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 6 cut(s) 42, 124, 213, 248, 345, 716
Bsc4I CCNNNNNNNGG 1 cut(s) 662
Bse118I RCCGGY 1 cut(s) 185
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 6 cut(s) 42, 124, 213, 248, 345, 716
BseGI GGATG 2 cut(s) 510, 643
BseLI CCNNNNNNNGG 1 cut(s) 662
BseMII CTCAG 3 cut(s) 11, 326, 401
BseXI GCAGC 5 cut(s) 17, 36, 39, 289, 541
BseYI CCCAGC 1 cut(s) 464
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 2 cut(s) 247, 488
BshNI GGYRCC 1 cut(s) 176
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 196
BsiSI CCGG 3 cut(s) 186, 235, 345
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 1 cut(s) 662
BsmFI GGGAC 1 cut(s) 388
BsmI GAATGC 3 cut(s) 221, 670, 726
BsnI GGCC 2 cut(s) 247, 488
BsoBI CYCGRG 1 cut(s) 196
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 1 cut(s) 133
Bsp1720I GCTNAGC 1 cut(s) 20
BspACI CCGC 4 cut(s) 47, 266, 275, 330
BspANI GGCC 2 cut(s) 247, 488
BspCNI CTCAG 3 cut(s) 12, 325, 402
BspLI GGNNCC 2 cut(s) 178, 376
BspMI ACCTGC 1 cut(s) 259
BspT107I GGYRCC 1 cut(s) 176
BsrFI RCCGGY 1 cut(s) 185
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 6 cut(s) 42, 124, 213, 248, 345, 716
BssMI GATC 1 cut(s) 133
BssNI GRCGYC 1 cut(s) 189
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 230, 371
BstACI GRCGYC 1 cut(s) 189
BstC8I GCNNGC 4 cut(s) 187, 486, 521, 668
BstDEI CTNAG 3 cut(s) 20, 312, 410
BstDSI CCRYGG 3 cut(s) 42, 213, 248
BstEII GGTNACC 1 cut(s) 340
BstF5I GGATG 2 cut(s) 510, 643
BstH2I RGCGCY 2 cut(s) 81, 471
BstHHI GCGC 4 cut(s) 71, 80, 112, 470
BstKTI GATC 1 cut(s) 136
BstMBI GATC 1 cut(s) 133
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 9 cut(s) 47, 75, 77, 86, 265, 465, 551, 705, 732
BstNI CCWGG 1 cut(s) 125
BstNSI RCATGY 1 cut(s) 523
BstPI GGTNACC 1 cut(s) 340
BstSCI CCNGG 2 cut(s) 123, 344
BstV1I GCAGC 5 cut(s) 17, 36, 39, 289, 541
BstV2I GAAGAC 1 cut(s) 154
BsuRI GGCC 2 cut(s) 247, 488
BtgI CCRYGG 3 cut(s) 42, 213, 248
BtgZI GCGATG 1 cut(s) 23
BtrI CACGTC 1 cut(s) 501
BtsCI GGATG 2 cut(s) 510, 643
BtsIMutI CAGTG 2 cut(s) 71, 376
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 4 cut(s) 187, 486, 521, 668
CfoI GCGC 4 cut(s) 71, 80, 112, 470
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 2 cut(s) 245, 375
CseI GACGC 2 cut(s) 151, 178
Csp6I GTAC 2 cut(s) 208, 571
CviAII CATG 5 cut(s) 151, 292, 520, 620, 661
CviJI RGCY 9 cut(s) 41, 123, 129, 247, 302, 488, 545, 554, 735
CviKI_1 RGCY 9 cut(s) 41, 123, 129, 247, 302, 488, 545, 554, 735
CviQI GTAC 2 cut(s) 208, 571
DdeI CTNAG 3 cut(s) 20, 312, 410
DpnI GATC 1 cut(s) 135
DpnII GATC 1 cut(s) 133
DriI GACNNNNNGTC 1 cut(s) 154
Eam1105I GACNNNNNGTC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 375
Eco47III AGCGCT 1 cut(s) 469
Eco57I CTGAAG 2 cut(s) 307, 413
Eco88I CYCGRG 1 cut(s) 196
Eco91I GGTNACC 1 cut(s) 340
EcoO65I GGTNACC 1 cut(s) 340
EcoRII CCWGG 1 cut(s) 123
FaeI CATG 5 cut(s) 154, 295, 523, 623, 664
FaqI GGGAC 1 cut(s) 388
FatI CATG 5 cut(s) 150, 291, 519, 619, 660
Fnu4HI GCNGC 6 cut(s) 6, 25, 28, 48, 303, 555
FokI GGATG 2 cut(s) 497, 630
Fsp4HI GCNGC 6 cut(s) 6, 25, 28, 48, 303, 555
GlaI GCGC 4 cut(s) 70, 79, 111, 469
GluI GCNGC 6 cut(s) 6, 25, 28, 48, 303, 555
GsaI CCCAGC 1 cut(s) 468
GsuI CTGGAG 1 cut(s) 93
HaeII RGCGCY 2 cut(s) 81, 471
HaeIII GGCC 2 cut(s) 247, 488
HapII CCGG 3 cut(s) 186, 235, 345
HgaI GACGC 2 cut(s) 151, 178
HhaI GCGC 4 cut(s) 71, 80, 112, 470
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 5 cut(s) 154, 295, 523, 623, 664
Hin6I GCGC 4 cut(s) 69, 78, 110, 468
HinP1I GCGC 4 cut(s) 69, 78, 110, 468
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 4 cut(s) 61, 202, 534, 721
HpaII CCGG 3 cut(s) 186, 235, 345
HphI GGTGA 2 cut(s) 334, 499
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 6 cut(s) 315, 364, 411, 533, 562, 720
Hpy8I GTNNAC 1 cut(s) 352
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 3 cut(s) 100, 388, 485
HpyCH4III ACNGT 2 cut(s) 230, 371
HpyCH4IV ACGT 2 cut(s) 336, 500
HpyCH4V TGCA 3 cut(s) 453, 519, 746
HpyF10VI GCNNNNNNNGC 9 cut(s) 47, 75, 77, 86, 265, 465, 551, 705, 732
HpyF3I CTNAG 3 cut(s) 20, 312, 410
HpySE526I ACGT 2 cut(s) 336, 500
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 5 cut(s) 154, 295, 523, 623, 664
HspAI GCGC 4 cut(s) 69, 78, 110, 468
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
Kzo9I GATC 1 cut(s) 133
LmnI GCTCC 1 cut(s) 551
Lsp1109I GCAGC 5 cut(s) 17, 36, 39, 289, 541
LweI GCATC 1 cut(s) 6
MaeII ACGT 2 cut(s) 336, 500
MaeIII GTNAC 2 cut(s) 340, 505
MalI GATC 1 cut(s) 135
MboI GATC 1 cut(s) 133
MboII GAAGA 4 cut(s) 154, 313, 575, 684
MhlI GDGCHC 1 cut(s) 261
MluCI AATT 1 cut(s) 355
MnlI CCTC 7 cut(s) 68, 115, 203, 524, 657, 719, 726
MreI CGCCGGCG 1 cut(s) 185
MroNI GCCGGC 1 cut(s) 185
MseI TTAA 3 cut(s) 575, 627, 702
MspI CCGG 3 cut(s) 186, 235, 345
MspR9I CCNGG 2 cut(s) 125, 346
Mva1269I GAATGC 3 cut(s) 221, 670, 726
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 9 cut(s) 47, 75, 77, 86, 265, 465, 551, 705, 732
NaeI GCCGGC 1 cut(s) 187
NciI CCSGG 1 cut(s) 346
NdeII GATC 1 cut(s) 133
NgoMIV GCCGGC 1 cut(s) 185
NlaIII CATG 5 cut(s) 154, 295, 523, 623, 664
NlaIV GGNNCC 2 cut(s) 178, 376
NmuCI GTSAC 2 cut(s) 340, 505
NspI RCATGY 1 cut(s) 523
PaeI GCATGC 1 cut(s) 523
PaqCI CACCTGC 1 cut(s) 259
PctI GAATGC 3 cut(s) 221, 670, 726
PdiI GCCGGC 1 cut(s) 187
PfeI GAWTC 4 cut(s) 61, 202, 534, 721
PkrI GCNGC 6 cut(s) 7, 26, 29, 49, 304, 556
Psp6I CCWGG 1 cut(s) 123
PspEI GGTNACC 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 2 cut(s) 178, 376
PspPI GGNCC 2 cut(s) 245, 375
RsaI GTAC 2 cut(s) 209, 572
RsaNI GTAC 2 cut(s) 208, 571
SaqAI TTAA 3 cut(s) 575, 627, 702
SatI GCNGC 6 cut(s) 6, 25, 28, 48, 303, 555
Sau3AI GATC 1 cut(s) 133
Sau96I GGNCC 2 cut(s) 245, 375
ScaI AGTACT 1 cut(s) 572
ScrFI CCNGG 2 cut(s) 125, 346
SduI GDGCHC 1 cut(s) 261
SetI ASST 6 cut(s) 178, 273, 339, 503, 547, 556
SfaNI GCATC 1 cut(s) 6
SgrAI CRCCGGYG 1 cut(s) 185
SinI GGWCC 1 cut(s) 375
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
SphI GCATGC 1 cut(s) 523
Sse9I AATT 1 cut(s) 355
SsiI CCGC 4 cut(s) 47, 266, 275, 330
StyD4I CCNGG 2 cut(s) 123, 344
TaaI ACNGT 2 cut(s) 230, 371
TaiI ACGT 2 cut(s) 339, 503
TaqI TCGA 3 cut(s) 119, 140, 147
TasI AATT 1 cut(s) 355
TatI WGTACW 2 cut(s) 207, 570
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 4 cut(s) 61, 202, 534, 721
Tru1I TTAA 3 cut(s) 575, 627, 702
Tru9I TTAA 3 cut(s) 575, 627, 702
TscAI CASTG 2 cut(s) 71, 376
TseFI GTSAC 2 cut(s) 340, 505
TseI GCWGC 5 cut(s) 5, 24, 27, 302, 554
Tsp45I GTSAC 2 cut(s) 340, 505
TspDTI ATGAA 2 cut(s) 280, 555
TspGWI ACGGA 2 cut(s) 148, 202
TspRI CASTG 2 cut(s) 71, 376
VpaK11BI GGWCC 1 cut(s) 375
XceI RCATGY 1 cut(s) 523
ZrmI AGTACT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.