Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001991

Physical Location & Seq

Genomic Coordinates Reverse (-)

32332956 .. 32336013

Gene Structure 3058 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1185500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1587 bp

ATGGCAGCGATGGAGATGCTGAGCAGCAGCAAGTGGAAAGCCACGGCGGCAAGTATATGGATTCAGTGCGCTGGAGGCGCTTCCTACGCCTTCGGCATATATTCCTCTGCGCTCAAGTCGAGCCAGGGCTACGATCAATCGACGCTCGACATGGTGTCCGTCTTCAAGGACATAGGTGCCAACGCCGGCGTCCTCTCGGGGATTCTGTACTCCGTGGTCGCATTCAACGGTCGCCGGATTGGTGGGCCGTGGGTGGTGCTCGTGGCGGGGGCTCTTCAGTGCTTCGTGGGGTACTTCTTCCTTTGGGCTGCCGTCACCGGATTGGTAGAGACTCCGCAGGTGGCGGTTGTGTGTTTGTTCATGTTCTTGGCTGCCCACTCTCAGACTTTCTTCAGCACCGCCAACGTGGTCACCGGGGTCAACAATTTCTCTGATTACAGTGGGACCATCGTTGGCATACTGAAGGGATTTCTTGGTCTGAGTTCAGCACTTCTTGTCCAAGTCTATGACACATTCTGCAAAGGCAACCCCAGCGCTTTCCTTCTCCTGCTGGCCTTGTTTCCCACGTCTGTCACCATCCTCTGCATGCCTTTGGTCAGAATCTATGAAGCTGGTGGAGCTGCTTCTGACAAAAAGTACTTAAACATCTTCTCTGCTTTTGCTCTCATCACTTCTGCTTATCTCATGGTCTTAATCATATTCCAAAACATCCTCACTTTCCCATCATGGGCGAGCATTCTCACATTCACAGCACTTCTTCTTGCTTTAATCGCCTCGCCCCTCGGAATCGCATTCAAAGCCCAAAACTTGCAACCCAAGAAACTCCTTGAGGCACTCGATGATCCTTTGCCAGAAAACCCTGAGCCAGTTTCAGCATCCTCTGAACTTCATGTATCAGATCATGAAGATATGAATCTATTGAGAGCTACGCGTTGTGTCGATTTTTGGTTGCTGTTTATTGCTATGGTATGTGGGATGGGGTCTGGATTGGCCACCATAAACAACATGAGCCAAATTGGGGAATCTCTTGGCTACAAAACAGTTGAGATCAATTCCTTGGTTTCTCTGTGGAGCATATGGAATTTTCTAGGCCGGTTTGGAGCCGGGTTTCTCTCGGATTACTTGCTGCATAAGAAAGGATGGGCAAGGCCATTTCTGATGGCTGTCACCCTAGCAATCATGGCAGCCGGCCACATTGTGATTGCATCTAGTTTTTCGGGGAATTTGTACGTGGGTTCAATCTTAGTTGGGATTTGCTATGGCTCACAGTGGTCACTTATGCCCACAATCACTTCTGAGATCTTTGGGATAAGACACATGGGGACTATTTTCAACACAATAGCCATAGCCAGTCCTGTTGGTTCTTATATTTTCTCCGTCAGAGTAATCGGGTTCATATATGATAGAGAAGCTTCCGGAGAAGATGATAACTCCTGCAGCGGCACTCGCTGCTTCATGCTGTCTTTTTTTATCATGGCTTCTGTTGCTTTCTTTGGGTTTCTTGTTGCTCTTGCCTTGTTCTTTCGGACGAGGAGATTCTATCACTCGATCGTGATGAAAAGACTGCAGCATTCTTCTCCGAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1 Nodulin-like

Protein Analysis

529

Amino Acids

57.27

Weight (kDa)

8.26

Isoelectric Point (pI)

34.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nodulin-like	PF06813	11 - 264	1.8e-61	Nodulin-like
NFD4_C	PF23262	310 - 514	4.5e-24	NFD4, C-terminal domain
MFS_1	PF07690	318 - 512	2.1e-09	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001991)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18940 AT1G74780 AT2G34350 AT2G34350 AT2G34355 AT2G34355
fragaria_vesca	FvH4_5g15790 FvH4_5g15790 FvH4_5g15790 FvH4_5g15810
malus_domestica	MD00G1132000.v1.1 MD06G1185400.v1.1 MD06G1185500.v1.1 MD06G1185600.v1.1 MD14G1191700.v1.1
prunus_persica	Prupe.5G184300_v2.0.a1 Prupe.5G184300_v2.0.a1
pyrus_communis	pycom06g16490 pycom06g16500 pycom14g15940
rosa_chinensis	RchiOBHm_Chr7g0180321 RchiOBHm_Chr7g0180331
rosa_laevigata	RLG00000005276 RLG00000005277
rosa_multiflora	Rmu_sc0003526.1_g000013 Rmu_sc0003526.1_g000014 Rmu_sc0031066.1_g000002 Rmu_ssc0000099.1_g000002 Rmu_ssc0000099.1_g000004
rosa_roxburghii	Rroxscaffold_3G00272980 Rroxscaffold_3G00272990
rosa_rugosa	Rorug06G0432000 Rorug06G0432100 Rorug06G0432200 Rorug06G0432300
rosa_samantha	Rh7AG032600 Rh7AG032700 Rh7BG032600 Rh7BG032700 Rh7CG034500 Rh7CG034600 Rh7DG033200 Rh7DG033300
rosa_wichuraiana	Rw7G002720 Rw7G002730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	328
Acc36I	ACCTGC	1 cut(s)	328
AccB1I	GGYRCC	1 cut(s)	176
AccII	CGCG	1 cut(s)	931
AccIII	TCCGGA	1 cut(s)	1415
AciI	CCGC	6 cut(s)	47, 266, 335, 344, 399, 1440
AclWI	GGATC	1 cut(s)	836
AcoI	YGGCCR	2 cut(s)	990, 1189
AcsI	RAATTY	2 cut(s)	1081, 1222
AcuI	CTGAAG	3 cut(s)	260, 376, 482
AcyI	GRCGYC	1 cut(s)	189
AdeI	CACNNNGTG	1 cut(s)	1198
AfaI	GTAC	4 cut(s)	209, 293, 638, 1229
AfeI	AGCGCT	1 cut(s)	535
AfiI	CCNNNNNNNGG	2 cut(s)	727, 1018
AflIII	ACRYGT	1 cut(s)	929
AgsI	TTSAA	5 cut(s)	166, 226, 796, 1239, 1333
AhdI	GACNNNNNGTC	1 cut(s)	154
AjiI	CACGTC	1 cut(s)	567
AjnI	CCWGG	1 cut(s)	123
AluBI	AGCT	4 cut(s)	611, 620, 926, 1412
AluI	AGCT	4 cut(s)	611, 620, 926, 1412
Alw21I	GWGCWC	1 cut(s)	261
Alw26I	GTCTC	1 cut(s)	323
AlwI	GGATC	1 cut(s)	836
Ama87I	CYCGRG	1 cut(s)	196
Aor13HI	TCCGGA	1 cut(s)	1415
Aor51HI	AGCGCT	1 cut(s)	535
AoxI	GGCC	6 cut(s)	245, 552, 990, 1090, 1148, 1189
ApoI	RAATTY	2 cut(s)	1081, 1222
AspLEI	GCGC	4 cut(s)	71, 80, 112, 536
AspS9I	GGNCC	2 cut(s)	245, 444
AsuC2I	CCSGG	2 cut(s)	415, 1105
AsuHPI	GGTGA	4 cut(s)	307, 403, 565, 1159
AvaI	CYCGRG	1 cut(s)	196
AvaII	GGWCC	1 cut(s)	444
BalI	TGGCCA	1 cut(s)	992
BanI	GGYRCC	1 cut(s)	176
BanII	GRGCYC	1 cut(s)	274
BauI	CACGAG	1 cut(s)	260
BbsI	GAAGAC	1 cut(s)	154
Bbv12I	GWGCWC	1 cut(s)	261
BccI	CCATC	7 cut(s)	4, 455, 584, 730, 970, 1134, 1153
BceAI	ACGGC	3 cut(s)	60, 232, 296
BcgI	CGANNNNNNTGC	1 cut(s)	32
BciT130I	CCWGG	1 cut(s)	125
BcnI	CCSGG	2 cut(s)	415, 1105
BcoDI	GTCTC	1 cut(s)	323
BfaI	CTAG	3 cut(s)	1088, 1172, 1209
BfmI	CTRYAG	2 cut(s)	1435, 1565
BfoI	RGCGCY	2 cut(s)	81, 537
BfuAI	ACCTGC	1 cut(s)	328
BglI	GCCNNNNNGGC	1 cut(s)	47
BglII	AGATCT	1 cut(s)	1299
BlpI	GCTNAGC	1 cut(s)	20
BmcAI	AGTACT	1 cut(s)	638
Bme1390I	CCNGG	3 cut(s)	125, 415, 1105
Bme18I	GGWCC	1 cut(s)	444
BmeRI	GACNNNNNGTC	1 cut(s)	154
BmeT110I	CYCGRG	1 cut(s)	196
BmgBI	CACGTC	1 cut(s)	567
BmgT120I	GGNCC	2 cut(s)	245, 444
BmiI	GGNNCC	3 cut(s)	178, 445, 1102
BmrFI	CCNGG	3 cut(s)	125, 415, 1105
BmsI	GCATC	3 cut(s)	6, 884, 1214
BpiI	GAAGAC	1 cut(s)	154
BpmI	CTGGAG	1 cut(s)	93
Bpu10I	CCTNAGC	1 cut(s)	861
Bpu1102I	GCTNAGC	1 cut(s)	20
BpuEI	CTTGAG	2 cut(s)	98, 848
BpuMI	CCSGG	2 cut(s)	415, 1105
BsaAI	YACGTR	1 cut(s)	1231
BsaBI	GATNNNNATC	1 cut(s)	912
BsaHI	GRCGYC	1 cut(s)	189
BsaJI	CCNNGG	7 cut(s)	42, 124, 213, 248, 414, 781, 1056
BsaWI	WCCGGW	2 cut(s)	317, 1415
Bsc4I	CCNNNNNNNGG	2 cut(s)	727, 1018
Bse118I	RCCGGY	3 cut(s)	185, 1092, 1187
Bse1I	ACTGG	2 cut(s)	866, 1350
Bse8I	GATNNNNATC	1 cut(s)	912
BseAI	TCCGGA	1 cut(s)	1415
BseBI	CCWGG	1 cut(s)	125
BseDI	CCNNGG	7 cut(s)	42, 124, 213, 248, 414, 781, 1056
BseGI	GGATG	5 cut(s)	576, 708, 875, 981, 1145
BseJI	GATNNNNATC	1 cut(s)	912
BseLI	CCNNNNNNNGG	2 cut(s)	727, 1018
BseMII	CTCAG	5 cut(s)	11, 395, 470, 852, 1287
BseNI	ACTGG	2 cut(s)	866, 1350
BseRI	GAGGAG	1 cut(s)	1546
BseYI	CCCAGC	1 cut(s)	530
Bsh1236I	CGCG	1 cut(s)	931
Bsh1285I	CGRYCG	2 cut(s)	232, 1551
BshFI	GGCC	6 cut(s)	247, 554, 992, 1092, 1150, 1191
BshNI	GGYRCC	1 cut(s)	176
BsiEI	CGRYCG	2 cut(s)	232, 1551
BsiHKAI	GWGCWC	1 cut(s)	261
BsiHKCI	CYCGRG	1 cut(s)	196
BsiSI	CCGG	8 cut(s)	186, 235, 318, 414, 1093, 1104, 1188, 1416
BslFI	GGGAC	2 cut(s)	457, 1336
BslI	CCNNNNNNNGG	2 cut(s)	727, 1018
BsmAI	GTCTC	1 cut(s)	323
BsmFI	GGGAC	2 cut(s)	457, 1336
BsmI	GAATGC	4 cut(s)	221, 735, 791, 1570
BsnI	GGCC	6 cut(s)	247, 554, 992, 1092, 1150, 1191
BsoBI	CYCGRG	1 cut(s)	196
Bsp1286I	GDGCHC	2 cut(s)	261, 274
Bsp13I	TCCGGA	1 cut(s)	1415
Bsp143I	GATC	6 cut(s)	133, 841, 898, 1047, 1299, 1548
Bsp1720I	GCTNAGC	1 cut(s)	20
BspACI	CCGC	6 cut(s)	47, 266, 335, 344, 399, 1440
BspANI	GGCC	6 cut(s)	247, 554, 992, 1092, 1150, 1191
BspCNI	CTCAG	5 cut(s)	12, 394, 471, 853, 1288
BspEI	TCCGGA	1 cut(s)	1415
BspFNI	CGCG	1 cut(s)	931
BspHI	TCATGA	1 cut(s)	901
BspLI	GGNNCC	3 cut(s)	178, 445, 1102
BspMAI	CTGCAG	2 cut(s)	1439, 1569
BspMI	ACCTGC	1 cut(s)	328
BspPI	GGATC	1 cut(s)	836
BspQI	GCTCTTC	1 cut(s)	279
BspT107I	GGYRCC	1 cut(s)	176
BsrFI	RCCGGY	3 cut(s)	185, 1092, 1187
BsrI	ACTGG	2 cut(s)	866, 1350
BssAI	RCCGGY	3 cut(s)	185, 1092, 1187
BssECI	CCNNGG	7 cut(s)	42, 124, 213, 248, 414, 781, 1056
BssMI	GATC	6 cut(s)	133, 841, 898, 1047, 1299, 1548
BssNI	GRCGYC	1 cut(s)	189
BssSI	CACGAG	1 cut(s)	260
BssT1I	CCWWGG	1 cut(s)	1056
Bst2BI	CACGAG	1 cut(s)	260
Bst2UI	CCWGG	1 cut(s)	125
Bst4CI	ACNGT	4 cut(s)	230, 440, 1042, 1269
Bst6I	CTCTTC	1 cut(s)	279
BstACI	GRCGYC	1 cut(s)	189
BstAPI	GCANNNNNTGC	1 cut(s)	1449
BstBAI	YACGTR	1 cut(s)	1231
BstC8I	GCNNGC	5 cut(s)	187, 552, 587, 733, 1189
BstDEI	CTNAG	6 cut(s)	20, 381, 479, 861, 1243, 1296
BstDSI	CCRYGG	3 cut(s)	42, 213, 248
BstEII	GGTNACC	1 cut(s)	409
BstF5I	GGATG	5 cut(s)	576, 708, 875, 981, 1145
BstFNI	CGCG	1 cut(s)	931
BstH2I	RGCGCY	2 cut(s)	81, 537
BstHHI	GCGC	4 cut(s)	71, 80, 112, 536
BstKTI	GATC	6 cut(s)	136, 844, 901, 1050, 1302, 1551
BstMAI	GTCTC	1 cut(s)	323
BstMBI	GATC	6 cut(s)	133, 841, 898, 1047, 1299, 1548
BstMCI	CGRYCG	2 cut(s)	232, 1551
BstNI	CCWGG	1 cut(s)	125
BstNSI	RCATGY	1 cut(s)	589
BstPI	GGTNACC	1 cut(s)	409
BstSCI	CCNGG	3 cut(s)	123, 413, 1103
BstSFI	CTRYAG	2 cut(s)	1435, 1565
BstUI	CGCG	1 cut(s)	931
BstV2I	GAAGAC	1 cut(s)	154
BstX2I	RGATCY	1 cut(s)	1299
BstYI	RGATCY	1 cut(s)	1299
BsuRI	GGCC	6 cut(s)	247, 554, 992, 1092, 1150, 1191
BtgI	CCRYGG	3 cut(s)	42, 213, 248
BtgZI	GCGATG	1 cut(s)	23
BtrI	CACGTC	1 cut(s)	567
BtsCI	GGATG	5 cut(s)	576, 708, 875, 981, 1145
BtsIMutI	CAGTG	4 cut(s)	71, 284, 445, 1274
BveI	ACCTGC	1 cut(s)	328
Cac8I	GCNNGC	5 cut(s)	187, 552, 587, 733, 1189
CciI	TCATGA	1 cut(s)	901
CfoI	GCGC	4 cut(s)	71, 80, 112, 536
Cfr10I	RCCGGY	3 cut(s)	185, 1092, 1187
Cfr13I	GGNCC	2 cut(s)	245, 444
CseI	GACGC	2 cut(s)	151, 178
Csp6I	GTAC	4 cut(s)	208, 292, 637, 1228
CviQI	GTAC	4 cut(s)	208, 292, 637, 1228
DdeI	CTNAG	6 cut(s)	20, 381, 479, 861, 1243, 1296
DpnI	GATC	6 cut(s)	135, 843, 900, 1049, 1301, 1550
DpnII	GATC	6 cut(s)	133, 841, 898, 1047, 1299, 1548
DraIII	CACNNNGTG	1 cut(s)	1198
DriI	GACNNNNNGTC	1 cut(s)	154
EaeI	YGGCCR	2 cut(s)	990, 1189
Eam1104I	CTCTTC	1 cut(s)	279
Eam1105I	GACNNNNNGTC	1 cut(s)	154
EarI	CTCTTC	1 cut(s)	279
Eco130I	CCWWGG	1 cut(s)	1056
Eco24I	GRGCYC	1 cut(s)	274
Eco47I	GGWCC	1 cut(s)	444
Eco47III	AGCGCT	1 cut(s)	535
Eco57I	CTGAAG	3 cut(s)	260, 376, 482
Eco88I	CYCGRG	1 cut(s)	196
Eco91I	GGTNACC	1 cut(s)	409
EcoO65I	GGTNACC	1 cut(s)	409
EcoRII	CCWGG	1 cut(s)	123
EcoT14I	CCWWGG	1 cut(s)	1056
EcoT38I	GRGCYC	1 cut(s)	274
ErhI	CCWWGG	1 cut(s)	1056
FaqI	GGGAC	2 cut(s)	457, 1336
FauI	CCCGC	1 cut(s)	259
FauNDI	CATATG	1 cut(s)	1076
FokI	GGATG	5 cut(s)	563, 695, 862, 988, 1152
FriOI	GRGCYC	1 cut(s)	274
FspBI	CTAG	3 cut(s)	1088, 1172, 1209
GlaI	GCGC	4 cut(s)	70, 79, 111, 535
GsaI	CCCAGC	1 cut(s)	534
GsuI	CTGGAG	1 cut(s)	93
HaeII	RGCGCY	2 cut(s)	81, 537
HaeIII	GGCC	6 cut(s)	247, 554, 992, 1092, 1150, 1191
HapII	CCGG	8 cut(s)	186, 235, 318, 414, 1093, 1104, 1188, 1416
HgaI	GACGC	2 cut(s)	151, 178
HhaI	GCGC	4 cut(s)	71, 80, 112, 536
Hin1I	GRCGYC	1 cut(s)	189
Hin6I	GCGC	4 cut(s)	69, 78, 110, 534
HinP1I	GCGC	4 cut(s)	69, 78, 110, 534
HincII	GTYRAC	1 cut(s)	421
HindII	GTYRAC	1 cut(s)	421
HindIII	AAGCTT	1 cut(s)	1410
HinfI	GANTC	8 cut(s)	61, 202, 331, 600, 786, 913, 1022, 1536
HpaII	CCGG	8 cut(s)	186, 235, 318, 414, 1093, 1104, 1188, 1416
HphI	GGTGA	4 cut(s)	307, 403, 565, 1159
Hpy166II	GTNNAC	1 cut(s)	421
Hpy188III	TCNNGA	4 cut(s)	902, 984, 1416, 1552
Hpy8I	GTNNAC	1 cut(s)	421
Hpy99I	CGWCG	1 cut(s)	145
HpyAV	CCTTC	3 cut(s)	100, 457, 551
HpyCH4III	ACNGT	4 cut(s)	230, 440, 1042, 1269
HpyCH4IV	ACGT	3 cut(s)	405, 566, 1230
HpyCH4V	TGCA	7 cut(s)	519, 585, 811, 1129, 1205, 1437, 1567
HpyF3I	CTNAG	6 cut(s)	20, 381, 479, 861, 1243, 1296
HpySE526I	ACGT	3 cut(s)	405, 566, 1230
Hsp92I	GRCGYC	1 cut(s)	189
HspAI	GCGC	4 cut(s)	69, 78, 110, 534
Kpn2I	TCCGGA	1 cut(s)	1415
KroI	GCCGGC	2 cut(s)	185, 1187
KroNI	GCCGGC	2 cut(s)	187, 1189
Kzo9I	GATC	6 cut(s)	133, 841, 898, 1047, 1299, 1548
LguI	GCTCTTC	1 cut(s)	279
LmnI	GCTCC	3 cut(s)	617, 1071, 1100
LweI	GCATC	3 cut(s)	6, 884, 1214
MaeI	CTAG	3 cut(s)	1088, 1172, 1209
MaeII	ACGT	3 cut(s)	405, 566, 1230
MaeIII	GTNAC	5 cut(s)	313, 409, 571, 1165, 1272
MalI	GATC	6 cut(s)	135, 843, 900, 1049, 1301, 1550
MboI	GATC	6 cut(s)	133, 841, 898, 1047, 1299, 1548
MboII	GAAGA	9 cut(s)	154, 266, 289, 382, 640, 749, 917, 1433, 1566
MflI	RGATCY	1 cut(s)	1299
MhlI	GDGCHC	2 cut(s)	261, 274
MlsI	TGGCCA	1 cut(s)	992
MluCI	AATT	5 cut(s)	424, 1014, 1051, 1081, 1222
MluI	ACGCGT	1 cut(s)	929
MluNI	TGGCCA	1 cut(s)	992
MlyI	GAGTC	1 cut(s)	325
Mox20I	TGGCCA	1 cut(s)	992
MreI	CGCCGGCG	1 cut(s)	185
MroI	TCCGGA	1 cut(s)	1415
MroNI	GCCGGC	2 cut(s)	185, 1187
MscI	TGGCCA	1 cut(s)	992
MseI	TTAA	3 cut(s)	641, 692, 767
Msp20I	TGGCCA	1 cut(s)	992
MspA1I	CMGCKG	1 cut(s)	1440
MspI	CCGG	8 cut(s)	186, 235, 318, 414, 1093, 1104, 1188, 1416
MspR9I	CCNGG	3 cut(s)	125, 415, 1105
Mva1269I	GAATGC	4 cut(s)	221, 735, 791, 1570
MvaI	CCWGG	1 cut(s)	125
MvnI	CGCG	1 cut(s)	931
NaeI	GCCGGC	2 cut(s)	187, 1189
NciI	CCSGG	2 cut(s)	415, 1105
NdeI	CATATG	1 cut(s)	1076
NdeII	GATC	6 cut(s)	133, 841, 898, 1047, 1299, 1548
NgoMIV	GCCGGC	2 cut(s)	185, 1187
NlaIV	GGNNCC	3 cut(s)	178, 445, 1102
NmuCI	GTSAC	5 cut(s)	313, 409, 571, 1165, 1272
NspI	RCATGY	1 cut(s)	589
PaeI	GCATGC	1 cut(s)	589
PagI	TCATGA	1 cut(s)	901
PaqCI	CACCTGC	1 cut(s)	328
PciSI	GCTCTTC	1 cut(s)	279
PctI	GAATGC	4 cut(s)	221, 735, 791, 1570
PdiI	GCCGGC	2 cut(s)	187, 1189
PfeI	GAWTC	7 cut(s)	61, 202, 600, 786, 913, 1022, 1536
Ple19I	CGATCG	1 cut(s)	1551
PleI	GAGTC	1 cut(s)	325
PpsI	GAGTC	1 cut(s)	325
Ppu21I	YACGTR	1 cut(s)	1231
Psp6I	CCWGG	1 cut(s)	123
PspEI	GGTNACC	1 cut(s)	409
PspFI	CCCAGC	1 cut(s)	530
PspGI	CCWGG	1 cut(s)	123
PspN4I	GGNNCC	3 cut(s)	178, 445, 1102
PspPI	GGNCC	2 cut(s)	245, 444
PstI	CTGCAG	2 cut(s)	1439, 1569
PsuI	RGATCY	1 cut(s)	1299
PvuI	CGATCG	1 cut(s)	1551
RsaI	GTAC	4 cut(s)	209, 293, 638, 1229
RsaNI	GTAC	4 cut(s)	208, 292, 637, 1228
SapI	GCTCTTC	1 cut(s)	279
SaqAI	TTAA	3 cut(s)	641, 692, 767
Sau3AI	GATC	6 cut(s)	133, 841, 898, 1047, 1299, 1548
Sau96I	GGNCC	2 cut(s)	245, 444
ScaI	AGTACT	1 cut(s)	638
SchI	GAGTC	1 cut(s)	325
ScrFI	CCNGG	3 cut(s)	125, 415, 1105
SduI	GDGCHC	2 cut(s)	261, 274
SetI	ASST	9 cut(s)	178, 342, 408, 569, 613, 622, 928, 1233, 1414
SfaNI	GCATC	3 cut(s)	6, 884, 1214
SfcI	CTRYAG	2 cut(s)	1435, 1565
SgrAI	CRCCGGYG	1 cut(s)	185
SinI	GGWCC	1 cut(s)	444
SmlI	CTYRAG	2 cut(s)	113, 827
SmoI	CTYRAG	2 cut(s)	113, 827
SphI	GCATGC	1 cut(s)	589
Sse9I	AATT	5 cut(s)	424, 1014, 1051, 1081, 1222
SsiI	CCGC	6 cut(s)	47, 266, 335, 344, 399, 1440
SspMI	CTAG	3 cut(s)	1088, 1172, 1209
StyD4I	CCNGG	3 cut(s)	123, 413, 1103
StyI	CCWWGG	1 cut(s)	1056
TaaI	ACNGT	4 cut(s)	230, 440, 1042, 1269
TaiI	ACGT	3 cut(s)	408, 569, 1233
TaqI	TCGA	6 cut(s)	119, 140, 147, 837, 939, 1547
TasI	AATT	5 cut(s)	424, 1014, 1051, 1081, 1222
TatI	WGTACW	2 cut(s)	207, 636
TauI	GCSGC	2 cut(s)	50, 1443
TfiI	GAWTC	7 cut(s)	61, 202, 600, 786, 913, 1022, 1536
Tru1I	TTAA	3 cut(s)	641, 692, 767
Tru9I	TTAA	3 cut(s)	641, 692, 767
TscAI	CASTG	4 cut(s)	71, 284, 445, 1274
TseFI	GTSAC	5 cut(s)	313, 409, 571, 1165, 1272
Tsp45I	GTSAC	5 cut(s)	313, 409, 571, 1165, 1272
TspDTI	ATGAA	8 cut(s)	349, 621, 878, 918, 926, 1384, 1444, 1571
TspGWI	ACGGA	3 cut(s)	148, 202, 1366
TspRI	CASTG	4 cut(s)	71, 284, 445, 1274
VpaK11BI	GGWCC	1 cut(s)	444
XapI	RAATTY	2 cut(s)	1081, 1222
XceI	RCATGY	1 cut(s)	589
XspI	CTAG	3 cut(s)	1088, 1172, 1209
ZrmI	AGTACT	1 cut(s)	638

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.