Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001991

Physical Location & Seq

Genomic Coordinates Reverse (-)

32327995 .. 32330750

Gene Structure 2756 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1185400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1653 bp

ATGTACGGCAGTTCACTGAATCTCTTGGACAGCAAATGGTTCTCTACGGCGGCGAGCATATGGATACAGTGCACCAGCGGCTCCCTCTACACCTTCTCCGTCTACTCTCAGGCCATCAAGACAACGCAGGGCTACGACCAGTCAACGCTCGACACCGTCTCTGTCTTCAAGGACTTCGGAGCCAACTGTGGCGTCCTCTCTGGCCTCCTCTACTCGTACTCCGCCAGGTGGCGAAAGCTCTGGTTGGTCCTCCTGGCCGGGGCGGCCCAGTGCTTTTTGGGTTATTTTCTAATGTGGGCCGCCGTAGCCGGCGTTATTCCCCGGCCTTCGGTGCCTGTGATGTGTCTGTTCATGTTCGTGGCGGCTCATGCGGTGTCGTTCTTTAATACCTCTAATGTGGTCACTGGCGTCAGGAATTTTCCTTATTATAAAGGCACCATCGTTGGCATCATGAAGGGGTTTCTCGGTCTGAGTGGAGCAATCTTGATTCAAATATACCGGACATTTTTGCACAAGAAACCGGCTTCATTTCTTCTGATGCTGGCACTGCTACCTACCGTAAACACATTGTTGCTTATGTGGTTTGTGAGAATCCACAACAAGTGCGAAGGAGACGAGAAGAAGCACCTAAATAGTTTGTCGCTGATTGCGCTTGTTGTTGCAGCTTATCTTATGGGGTTTATAATTTTAGAGAACATCCTTACTTTGCGGTTTGCCATACGCTTCGTTGCTTTTGTTGCACTTCTGCTACTGCTTGCCTCACCTCTTTGCATTGCGATTAGAGCGCATAAAAGGGAGTCTGATAGAGTTTCACAGACTTTATTAGTTGAGGGTGGCCAGTTAACAGATACCGAGGTCAACTGGCGAGATAGTGGGAAAAGGAATGCGCAAGATCGTAGTTCTGGGTACCGTCATTTGCCTAGCAATACTGATCAAGAGTTGGATATTAACGATGAAAATACTTTGCAGTTGGGAGAAACTTTTAATCTACTCCAAGCTATGTGCACTATGGACTTCTGGATTTTGTTCATTGCCATGGCATGTGGAATGGGATCGGGACTTGCCACAGTGAATAACCTCAACCAGATAGGAAAATCGCTTGGTTACTCAACCTTTGAAACAGGTAGTTTGATTTCTTTGTGGAGCATCTGGAATTTCGTCGGGCGGTTTGGAGCTGGTTATGTATCAGATTATTTCCTGCATGCGAAAGGATTGGCGAGGCCATTGTTCATGGTCATCACTCTAGCAGGCATGAGCGTTGGTCACGTAGTGATTGCTTCCGGTATGCCTAGCGCTCTTTATGCTGGATCCTTACTGGTGGGCGTCTTTTATGGTTCTCAATGGTCACTAATGCCCACAATCGCTTCTGAGATATTCGGCGTGCTACATATGGGCACCATATTCAACGCCATTACAATAGCCGCGCCTGTAGGATCTTATATCTTTTCTGTGAGAGTGATCGGCCATTTTTATGACCAAGAAGCGTCGGGAGCTTGGGATACATGCGCCGGAATTCGCTGCTTTAGGTTATCATTCCTTATTATGGCATGTGCTACCGTCTTCGGATCTCTCACTGCCTGGGCTTTGTTCTTTCGGACACGAAGTTTCTACGGTCGGGTAATCCGACGACTGCTATGTTCTGTGCCAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nodulin-like

Protein Analysis

551

Amino Acids

60.63

Weight (kDa)

9.13

Isoelectric Point (pI)

33.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nodulin-like	PF06813	12 - 260	2e-66	Nodulin-like
NFD4_C	PF23262	335 - 537	1.3e-17	NFD4, C-terminal domain
MFS_1	PF07690	342 - 534	1e-09	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001991)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18940 AT1G74780 AT2G34350 AT2G34350 AT2G34355 AT2G34355
fragaria_vesca	FvH4_5g15790 FvH4_5g15790 FvH4_5g15790 FvH4_5g15810
malus_domestica	MD00G1132000.v1.1 MD06G1185400.v1.1 MD06G1185500.v1.1 MD06G1185600.v1.1 MD14G1191700.v1.1
prunus_persica	Prupe.5G184300_v2.0.a1 Prupe.5G184300_v2.0.a1
pyrus_communis	pycom06g16490 pycom06g16500 pycom14g15940
rosa_chinensis	RchiOBHm_Chr7g0180321 RchiOBHm_Chr7g0180331
rosa_laevigata	RLG00000005276 RLG00000005277
rosa_multiflora	Rmu_sc0003526.1_g000013 Rmu_sc0003526.1_g000014 Rmu_sc0031066.1_g000002 Rmu_ssc0000099.1_g000002 Rmu_ssc0000099.1_g000004
rosa_roxburghii	Rroxscaffold_3G00272980 Rroxscaffold_3G00272990
rosa_rugosa	Rorug06G0432000 Rorug06G0432100 Rorug06G0432200 Rorug06G0432300
rosa_samantha	Rh7AG032600 Rh7AG032700 Rh7BG032600 Rh7BG032700 Rh7CG034500 Rh7CG034600 Rh7DG033200 Rh7DG033300
rosa_wichuraiana	Rw7G002720 Rw7G002730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	429, 683
Acc16I	TGCGCA	1 cut(s)	888
Acc65I	GGTACC	1 cut(s)	906
AccB1I	GGYRCC	4 cut(s)	331, 434, 906, 1394
AccI	GTMKAC	1 cut(s)	102
AccII	CGCG	1 cut(s)	1424
AclWI	GGATC	5 cut(s)	1060, 1302, 1315, 1441, 1573
AcoI	YGGCCR	3 cut(s)	255, 835, 1462
AcsI	RAATTY	3 cut(s)	415, 1153, 1512
AcyI	GRCGYC	3 cut(s)	192, 408, 1323
AdeI	CACNNNGTG	1 cut(s)	1270
AfaI	GTAC	3 cut(s)	5, 218, 908
AfeI	AGCGCT	1 cut(s)	1294
AfiI	CCNNNNNNNGG	4 cut(s)	228, 259, 328, 1543
AgsI	TTSAA	4 cut(s)	169, 491, 1118, 1405
AjnI	CCWGG	3 cut(s)	224, 252, 1577
AluBI	AGCT	5 cut(s)	238, 665, 998, 1175, 1493
AluI	AGCT	5 cut(s)	238, 665, 998, 1175, 1493
Alw21I	GWGCWC	2 cut(s)	74, 1007
Alw26I	GTCTC	2 cut(s)	163, 606
Alw44I	GTGCAC	2 cut(s)	70, 1003
AlwI	GGATC	5 cut(s)	1060, 1302, 1315, 1441, 1573
Aor51HI	AGCGCT	1 cut(s)	1294
AoxI	GGCC	9 cut(s)	111, 202, 255, 264, 297, 323, 835, 1220, 1462
ApaLI	GTGCAC	2 cut(s)	70, 1003
ApeKI	GCWGC	2 cut(s)	662, 1518
ApoI	RAATTY	3 cut(s)	415, 1153, 1512
Asp718I	GGTACC	1 cut(s)	906
AspLEI	GCGC	6 cut(s)	652, 787, 889, 1295, 1426, 1508
AspS9I	GGNCC	3 cut(s)	247, 265, 297
AsuC2I	CCSGG	2 cut(s)	259, 322
AsuHPI	GGTGA	1 cut(s)	753
AvaII	GGWCC	1 cut(s)	247
BaeGI	GKGCMC	3 cut(s)	74, 1007, 1397
BaeI	ACNNNNGTAYC	2 cut(s)	840, 873
BalI	TGGCCA	1 cut(s)	837
BamHI	GGATCC	1 cut(s)	1307
BanI	GGYRCC	4 cut(s)	331, 434, 906, 1394
BbsI	GAAGAC	2 cut(s)	157, 1552
Bbv12I	GWGCWC	2 cut(s)	74, 1007
BbvI	GCAGC	2 cut(s)	674, 1505
BccI	CCATC	2 cut(s)	122, 446
BceAI	ACGGC	3 cut(s)	22, 63, 287
BciT130I	CCWGG	3 cut(s)	226, 254, 1579
BciVI	GTATCC	2 cut(s)	57, 1492
BclI	TGATCA	1 cut(s)	931
BcnI	CCSGG	2 cut(s)	259, 322
BcoDI	GTCTC	2 cut(s)	163, 606
BfaI	CTAG	4 cut(s)	921, 1244, 1290, 1651
BfmI	CTRYAG	1 cut(s)	1428
BfoI	RGCGCY	1 cut(s)	1296
BfuI	GTATCC	2 cut(s)	57, 1492
BglI	GCCNNNNNGGC	1 cut(s)	263
BisI	GCNGC	8 cut(s)	51, 79, 264, 300, 363, 663, 1422, 1519
BlsI	GCNGC	8 cut(s)	52, 80, 265, 301, 364, 664, 1423, 1520
Bme1390I	CCNGG	5 cut(s)	226, 254, 259, 322, 1579
Bme18I	GGWCC	1 cut(s)	247
BmgT120I	GGNCC	3 cut(s)	247, 265, 297
BmiI	GGNNCC	7 cut(s)	82, 181, 333, 436, 908, 1309, 1396
BmrFI	CCNGG	5 cut(s)	226, 254, 259, 322, 1579
BmrI	ACTGGG	1 cut(s)	262
BmsI	GCATC	3 cut(s)	456, 528, 1155
BmuI	ACTGGG	1 cut(s)	262
BpiI	GAAGAC	2 cut(s)	157, 1552
BpuMI	CCSGG	2 cut(s)	259, 322
BsaAI	YACGTR	1 cut(s)	1267
BsaHI	GRCGYC	3 cut(s)	192, 408, 1323
BsaJI	CCNNGG	5 cut(s)	258, 320, 852, 1035, 1578
BsaWI	WCCGGW	2 cut(s)	498, 1280
BsaXI	ACNNNNNCTCC	4 cut(s)	80, 110, 203, 233
Bsc4I	CCNNNNNNNGG	4 cut(s)	228, 259, 328, 1543
Bse118I	RCCGGY	2 cut(s)	308, 520
Bse1I	ACTGG	6 cut(s)	139, 268, 409, 838, 866, 1320
Bse3DI	GCAATG	2 cut(s)	771, 1029
BseBI	CCWGG	3 cut(s)	226, 254, 1579
BseDI	CCNNGG	5 cut(s)	258, 320, 852, 1035, 1578
BseGI	GGATG	1 cut(s)	696
BseLI	CCNNNNNNNGG	4 cut(s)	228, 259, 328, 1543
BseMI	GCAATG	2 cut(s)	771, 1029
BseMII	CTCAG	3 cut(s)	122, 461, 1359
BseNI	ACTGG	6 cut(s)	139, 268, 409, 838, 866, 1320
BseRI	GAGGAG	1 cut(s)	197
BseSI	GKGCMC	3 cut(s)	74, 1007, 1397
BseXI	GCAGC	2 cut(s)	674, 1505
Bsh1236I	CGCG	1 cut(s)	1424
Bsh1285I	CGRYCG	1 cut(s)	1615
BshFI	GGCC	9 cut(s)	113, 204, 257, 266, 299, 325, 837, 1222, 1464
BshNI	GGYRCC	4 cut(s)	331, 434, 906, 1394
BsiEI	CGRYCG	1 cut(s)	1615
BsiHKAI	GWGCWC	2 cut(s)	74, 1007
BsiSI	CCGG	7 cut(s)	258, 309, 322, 499, 521, 1281, 1509
BslFI	GGGAC	1 cut(s)	1071
BslI	CCNNNNNNNGG	4 cut(s)	228, 259, 328, 1543
BsmAI	GTCTC	2 cut(s)	163, 606
BsmBI	CGTCTC	2 cut(s)	163, 606
BsmFI	GGGAC	1 cut(s)	1071
BsmI	GAATGC	1 cut(s)	889
BsnI	GGCC	9 cut(s)	113, 204, 257, 266, 299, 325, 837, 1222, 1464
Bsp1286I	GDGCHC	3 cut(s)	74, 1007, 1397
Bsp143I	GATC	7 cut(s)	892, 931, 1052, 1307, 1433, 1458, 1565
Bsp19I	CCATGG	1 cut(s)	1035
BspANI	GGCC	9 cut(s)	113, 204, 257, 266, 299, 325, 837, 1222, 1464
BspCNI	CTCAG	3 cut(s)	121, 462, 1360
BspFNI	CGCG	1 cut(s)	1424
BspHI	TCATGA	1 cut(s)	450
BspLI	GGNNCC	7 cut(s)	82, 181, 333, 436, 908, 1309, 1396
BspPI	GGATC	5 cut(s)	1060, 1302, 1315, 1441, 1573
BspT107I	GGYRCC	4 cut(s)	331, 434, 906, 1394
BsrDI	GCAATG	2 cut(s)	771, 1029
BsrFI	RCCGGY	2 cut(s)	308, 520
BsrI	ACTGG	6 cut(s)	139, 268, 409, 838, 866, 1320
BssAI	RCCGGY	2 cut(s)	308, 520
BssECI	CCNNGG	5 cut(s)	258, 320, 852, 1035, 1578
BssMI	GATC	7 cut(s)	892, 931, 1052, 1307, 1433, 1458, 1565
BssNI	GRCGYC	3 cut(s)	192, 408, 1323
BssT1I	CCWWGG	1 cut(s)	1035
Bst2UI	CCWGG	3 cut(s)	226, 254, 1579
Bst4CI	ACNGT	8 cut(s)	69, 157, 188, 559, 911, 1069, 1558, 1613
BstACI	GRCGYC	3 cut(s)	192, 408, 1323
BstBAI	YACGTR	1 cut(s)	1267
BstC8I	GCNNGC	7 cut(s)	55, 310, 543, 756, 1203, 1249, 1382
BstDEI	CTNAG	3 cut(s)	108, 470, 1368
BstDSI	CCRYGG	1 cut(s)	1035
BstF5I	GGATG	1 cut(s)	696
BstFNI	CGCG	1 cut(s)	1424
BstH2I	RGCGCY	1 cut(s)	1296
BstHHI	GCGC	6 cut(s)	652, 787, 889, 1295, 1426, 1508
BstKTI	GATC	7 cut(s)	895, 934, 1055, 1310, 1436, 1461, 1568
BstMAI	GTCTC	2 cut(s)	163, 606
BstMBI	GATC	7 cut(s)	892, 931, 1052, 1307, 1433, 1458, 1565
BstMCI	CGRYCG	1 cut(s)	1615
BstNI	CCWGG	3 cut(s)	226, 254, 1579
BstNSI	RCATGY	4 cut(s)	1044, 1205, 1506, 1551
BstSCI	CCNGG	5 cut(s)	224, 252, 257, 320, 1577
BstSFI	CTRYAG	1 cut(s)	1428
BstSLI	GKGCMC	3 cut(s)	74, 1007, 1397
BstUI	CGCG	1 cut(s)	1424
BstV1I	GCAGC	2 cut(s)	674, 1505
BstV2I	GAAGAC	2 cut(s)	157, 1552
BstX2I	RGATCY	3 cut(s)	1307, 1433, 1565
BstYI	RGATCY	3 cut(s)	1307, 1433, 1565
BsuI	GTATCC	2 cut(s)	57, 1492
BsuRI	GGCC	9 cut(s)	113, 204, 257, 266, 299, 325, 837, 1222, 1464
BtgI	CCRYGG	1 cut(s)	1035
BtsCI	GGATG	1 cut(s)	696
BtsI	GCAGTG	2 cut(s)	545, 1572
BtsIMutI	CAGTG	7 cut(s)	14, 74, 275, 402, 545, 1074, 1572
Cac8I	GCNNGC	7 cut(s)	55, 310, 543, 756, 1203, 1249, 1382
CciI	TCATGA	1 cut(s)	450
CfoI	GCGC	6 cut(s)	652, 787, 889, 1295, 1426, 1508
Cfr10I	RCCGGY	2 cut(s)	308, 520
Cfr13I	GGNCC	3 cut(s)	247, 265, 297
CseI	GACGC	4 cut(s)	181, 397, 1312, 1473
Csp6I	GTAC	3 cut(s)	4, 217, 907
CviQI	GTAC	3 cut(s)	4, 217, 907
DdeI	CTNAG	3 cut(s)	108, 470, 1368
DpnI	GATC	7 cut(s)	894, 933, 1054, 1309, 1435, 1460, 1567
DpnII	GATC	7 cut(s)	892, 931, 1052, 1307, 1433, 1458, 1565
DraIII	CACNNNGTG	1 cut(s)	1270
EaeI	YGGCCR	3 cut(s)	255, 835, 1462
EciI	GGCGGA	1 cut(s)	211
Eco130I	CCWWGG	1 cut(s)	1035
Eco47I	GGWCC	1 cut(s)	247
Eco47III	AGCGCT	1 cut(s)	1294
EcoRI	GAATTC	1 cut(s)	1512
EcoRII	CCWGG	3 cut(s)	224, 252, 1577
EcoT14I	CCWWGG	1 cut(s)	1035
ErhI	CCWWGG	1 cut(s)	1035
Esp3I	CGTCTC	2 cut(s)	163, 606
FaqI	GGGAC	1 cut(s)	1071
FauNDI	CATATG	2 cut(s)	59, 1389
FbaI	TGATCA	1 cut(s)	931
FblI	GTMKAC	1 cut(s)	102
Fnu4HI	GCNGC	8 cut(s)	51, 79, 264, 300, 363, 663, 1422, 1519
FokI	GGATG	1 cut(s)	683
Fsp4HI	GCNGC	8 cut(s)	51, 79, 264, 300, 363, 663, 1422, 1519
FspBI	CTAG	4 cut(s)	921, 1244, 1290, 1651
FspI	TGCGCA	1 cut(s)	888
GlaI	GCGC	6 cut(s)	651, 786, 888, 1294, 1425, 1507
GluI	GCNGC	8 cut(s)	51, 79, 264, 300, 363, 663, 1422, 1519
HaeII	RGCGCY	1 cut(s)	1296
HaeIII	GGCC	9 cut(s)	113, 204, 257, 266, 299, 325, 837, 1222, 1464
HapII	CCGG	7 cut(s)	258, 309, 322, 499, 521, 1281, 1509
HgaI	GACGC	4 cut(s)	181, 397, 1312, 1473
HhaI	GCGC	6 cut(s)	652, 787, 889, 1295, 1426, 1508
Hin1I	GRCGYC	3 cut(s)	192, 408, 1323
Hin6I	GCGC	6 cut(s)	650, 785, 887, 1293, 1424, 1506
HinP1I	GCGC	6 cut(s)	650, 785, 887, 1293, 1424, 1506
HincII	GTYRAC	3 cut(s)	144, 843, 859
HindII	GTYRAC	3 cut(s)	144, 843, 859
HinfI	GANTC	4 cut(s)	19, 487, 591, 797
HpaI	GTTAAC	1 cut(s)	843
HpaII	CCGG	7 cut(s)	258, 309, 322, 499, 521, 1281, 1509
HphI	GGTGA	1 cut(s)	753
Hpy166II	GTNNAC	8 cut(s)	14, 72, 103, 144, 562, 843, 859, 1005
Hpy188I	TCNGA	9 cut(s)	179, 471, 537, 802, 1189, 1369, 1565, 1596, 1625
Hpy188III	TCNNGA	9 cut(s)	118, 412, 451, 484, 935, 1018, 1056, 1150, 1488
Hpy8I	GTNNAC	8 cut(s)	14, 72, 103, 144, 562, 843, 859, 1005
Hpy99I	CGWCG	3 cut(s)	1163, 1489, 1629
HpyAV	CCTTC	4 cut(s)	103, 336, 448, 602
HpyCH4III	ACNGT	8 cut(s)	69, 157, 188, 559, 911, 1069, 1558, 1613
HpyCH4IV	ACGT	1 cut(s)	1266
HpyCH4V	TGCA	8 cut(s)	72, 511, 662, 740, 771, 967, 1005, 1201
HpyF3I	CTNAG	3 cut(s)	108, 470, 1368
HpySE526I	ACGT	1 cut(s)	1266
Hsp92I	GRCGYC	3 cut(s)	192, 408, 1323
HspAI	GCGC	6 cut(s)	650, 785, 887, 1293, 1424, 1506
KpnI	GGTACC	1 cut(s)	910
KroI	GCCGGC	1 cut(s)	308
KroNI	GCCGGC	1 cut(s)	310
Ksp22I	TGATCA	1 cut(s)	931
KspAI	GTTAAC	1 cut(s)	843
Kzo9I	GATC	7 cut(s)	892, 931, 1052, 1307, 1433, 1458, 1565
LmnI	GCTCC	6 cut(s)	86, 179, 476, 1143, 1172, 1490
Lsp1109I	GCAGC	2 cut(s)	674, 1505
LweI	GCATC	3 cut(s)	456, 528, 1155
MaeI	CTAG	4 cut(s)	921, 1244, 1290, 1651
MaeII	ACGT	1 cut(s)	1266
MaeIII	GTNAC	4 cut(s)	400, 1103, 1262, 1344
MalI	GATC	7 cut(s)	894, 933, 1054, 1309, 1435, 1460, 1567
MboI	GATC	7 cut(s)	892, 931, 1052, 1307, 1433, 1458, 1565
MboII	GAAGA	4 cut(s)	157, 524, 631, 1552
MflI	RGATCY	3 cut(s)	1307, 1433, 1565
MhlI	GDGCHC	3 cut(s)	74, 1007, 1397
MlsI	TGGCCA	1 cut(s)	837
MluCI	AATT	4 cut(s)	415, 684, 1153, 1512
MluNI	TGGCCA	1 cut(s)	837
MlyI	GAGTC	1 cut(s)	806
MmeI	TCCRAC	2 cut(s)	921, 1648
Mox20I	TGGCCA	1 cut(s)	837
MroNI	GCCGGC	1 cut(s)	308
MscI	TGGCCA	1 cut(s)	837
MseI	TTAA	4 cut(s)	384, 842, 948, 984
MslI	CAYNNNNRTG	3 cut(s)	356, 1034, 1470
Msp20I	TGGCCA	1 cut(s)	837
MspA1I	CMGCKG	1 cut(s)	78
MspI	CCGG	7 cut(s)	258, 309, 322, 499, 521, 1281, 1509
MspR9I	CCNGG	5 cut(s)	226, 254, 259, 322, 1579
Mva1269I	GAATGC	1 cut(s)	889
MvaI	CCWGG	3 cut(s)	226, 254, 1579
MvnI	CGCG	1 cut(s)	1424
NaeI	GCCGGC	1 cut(s)	310
NciI	CCSGG	2 cut(s)	259, 322
NcoI	CCATGG	1 cut(s)	1035
NdeI	CATATG	2 cut(s)	59, 1389
NdeII	GATC	7 cut(s)	892, 931, 1052, 1307, 1433, 1458, 1565
NgoMIV	GCCGGC	1 cut(s)	308
NlaIV	GGNNCC	7 cut(s)	82, 181, 333, 436, 908, 1309, 1396
NmuCI	GTSAC	3 cut(s)	400, 1262, 1344
NsbI	TGCGCA	1 cut(s)	888
NspI	RCATGY	4 cut(s)	1044, 1205, 1506, 1551
PaeI	GCATGC	1 cut(s)	1205
PagI	TCATGA	1 cut(s)	450
PcsI	WCGNNNNNNNCGW	1 cut(s)	1621
PctI	GAATGC	1 cut(s)	889
PdiI	GCCGGC	1 cut(s)	310
PfeI	GAWTC	3 cut(s)	19, 487, 591
PflFI	GACNNNGTC	1 cut(s)	155
PkrI	GCNGC	8 cut(s)	52, 80, 265, 301, 364, 664, 1423, 1520
PleI	GAGTC	1 cut(s)	805
PpsI	GAGTC	1 cut(s)	805
Ppu21I	YACGTR	1 cut(s)	1267
PsiI	TTATAA	2 cut(s)	429, 683
Psp6I	CCWGG	3 cut(s)	224, 252, 1577
PspGI	CCWGG	3 cut(s)	224, 252, 1577
PspN4I	GGNNCC	7 cut(s)	82, 181, 333, 436, 908, 1309, 1396
PspPI	GGNCC	3 cut(s)	247, 265, 297
PsuI	RGATCY	3 cut(s)	1307, 1433, 1565
PsyI	GACNNNGTC	1 cut(s)	155
RsaI	GTAC	3 cut(s)	5, 218, 908
RsaNI	GTAC	3 cut(s)	4, 217, 907
RseI	CAYNNNNRTG	3 cut(s)	356, 1034, 1470
SaqAI	TTAA	4 cut(s)	384, 842, 948, 984
SatI	GCNGC	8 cut(s)	51, 79, 264, 300, 363, 663, 1422, 1519
Sau3AI	GATC	7 cut(s)	892, 931, 1052, 1307, 1433, 1458, 1565
Sau96I	GGNCC	3 cut(s)	247, 265, 297
SchI	GAGTC	1 cut(s)	806
ScrFI	CCNGG	5 cut(s)	226, 254, 259, 322, 1579
SduI	GDGCHC	3 cut(s)	74, 1007, 1397
SfaNI	GCATC	3 cut(s)	456, 528, 1155
SfcI	CTRYAG	1 cut(s)	1428
SfiI	GGCCNNNNNGGCC	1 cut(s)	263
SinI	GGWCC	1 cut(s)	247
SmiMI	CAYNNNNRTG	3 cut(s)	356, 1034, 1470
SphI	GCATGC	1 cut(s)	1205
Sse9I	AATT	4 cut(s)	415, 684, 1153, 1512
SspMI	CTAG	4 cut(s)	921, 1244, 1290, 1651
StyD4I	CCNGG	5 cut(s)	224, 252, 257, 320, 1577
StyI	CCWWGG	1 cut(s)	1035
TaaI	ACNGT	8 cut(s)	69, 157, 188, 559, 911, 1069, 1558, 1613
TaiI	ACGT	1 cut(s)	1269
TaqI	TCGA	1 cut(s)	150
TaqII	GACCGA	1 cut(s)	455
TasI	AATT	4 cut(s)	415, 684, 1153, 1512
TauI	GCSGC	6 cut(s)	53, 81, 266, 302, 365, 1424
TfiI	GAWTC	3 cut(s)	19, 487, 591
Tru1I	TTAA	4 cut(s)	384, 842, 948, 984
Tru9I	TTAA	4 cut(s)	384, 842, 948, 984
TscAI	CASTG	7 cut(s)	21, 74, 275, 409, 552, 1074, 1579
TseFI	GTSAC	3 cut(s)	400, 1262, 1344
TseI	GCWGC	2 cut(s)	662, 1518
Tsp45I	GTSAC	3 cut(s)	400, 1262, 1344
TspDTI	ATGAA	6 cut(s)	340, 467, 516, 969, 1018, 1219
TspGWI	ACGGA	1 cut(s)	88
TspRI	CASTG	7 cut(s)	21, 74, 275, 409, 552, 1074, 1579
Tth111I	GACNNNGTC	1 cut(s)	155
VneI	GTGCAC	2 cut(s)	70, 1003
VpaK11BI	GGWCC	1 cut(s)	247
XapI	RAATTY	3 cut(s)	415, 1153, 1512
XceI	RCATGY	4 cut(s)	1044, 1205, 1506, 1551
XmiI	GTMKAC	1 cut(s)	102
XspI	CTAG	4 cut(s)	921, 1244, 1290, 1651

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.