Rorug06G0432100

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001991
Physical Location & Seq
Forward (+)
56723654 .. 56725689
2036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0432100.1

Sequence Viewer

Length: 1662 bp
ATGGAAGCCCCTCTCTTTGCCCTTGGAATAACTCACATCTTGTCTGCATTTTATTCTCCAACTGGTTCTGAAATCAAACACGAAGTTGACAAGGTAACTCTCATGTTTGTTGCACTAGCCGGTGTTACTGTTCCAGTATATCTGTTGCAACACTACTTCTACACATTGATGGGGGAGTGGCTCACAAGTCGTGTGCGCCTATTAATGTTCTCAGCTATACTTTCAAACGAAGTTGGGTGGTTTGATTTGGATGAGAATAACACTGGTTCACTGACATCAATCCTAGCTGCCAATGCAAAATTAGTCCGAAGTGCTCTAGCTGACCGTCTATCAACAATAGTACAGAATTTATCACTCATAGCAACTGCATTTGTTATTTCCTTCACATTAAGTTGGCGTATTGCAGCTGTTGTGGTTGCTACCCTCCCCCTGCTAGTTGGAGCTTCCATTACTAAGGTCAACTACAATAGTGCTTATGCTAAAGCAACTTCCCTAGCTCGTGAAGCAATTGCCAACATACGTACTGTTGCTGCATTTGGGGCTGAGGATAGAATATCACTTCAGTTTGCATCAGAGCTAAACCAGCCAAATAAGAAAGCAGTTCTTCGAGGTCACATTTCCGGTTTTTTCTATGGCATATCGCAGTTTTTTTCATTCTGTTCCTATGCACTCGGCCTTTGGTATGCATCAGTTCTAATCAAGCACAAAAACTCTAATTTTGGAGACATCATGAAGTCCTTTATGGTATTGATTTTTACTGCACTGTGCATAGCAGAAACACTTGCTCTTGCTCCAGACATTGTGAAGGGGCCGCGGTCACTAGCACTCGGGTCGATTTTTAGTATTCTCAAAGGAGAAACAATCATACATTCTAATGATCCCACATCAAATGTGGTTGCTGTTATCAGAGGAGAAATAGAATTCAGAATTGTGAGCTTTTGGTACTCTACTCGGTCTGATATCACCATTTTCGACAACTTGAATTTGAAAGTGTCAACTGGAAAGAGTCTTGCAGTTTTCGGTCGAAGTGGTTCGGGGAAGAGTACTGTAATTGCACTAGTAATGAGATTTTATGACCCTATTTTTGGAGCAGTTATGATTGATGGACATGATATCAAAGGCTTAAACTTGAAATCTTTGAGGAAGAAAATAGGACTAGTCCAGCAAGAGCCAGAATTGTTCTCAACAACAGTTTATGACAACATTAAGTATGGGAATGAGGAAGCATCCGAAGTTGAGGTCATGAGGGCGGCGAAAGCAGCAAATGCTCATGAATTCATAAGCAGAATGCCTGAAGGGTTGAAAACTCAAGTGGGAGAGAAAGGAGTGCAGTTATCTGGTGGGCAAAAACAGCGGGTGGCTATTGCTAGTGCTATACTGAAAGATCCATCAATTCTTCTTTTAGATGAAGTAACAAGTGCATTGGACACAGAGGCCGAGAAGCTGGTTCAGGAGGCTCTAGATAAGCTAATGGAAGGCCGAACCACTATTCTGGTAGCACACAGATTGTCAACTATTCATGATGCGAACAAGATTGCCCTGCTACAAAATGGTCGGGTGGTGGAAATGGGTAGCCATGAGCAACTGATTGGAACTCCCGGTAGTCTGTATAAGCAATTAGTTAGTCTACAGCAAGAGAAGAGTGAACAAGTGCTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

553

Amino Acids

60.43

Weight (kDa)

7.75

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 6 - 255 1.8e-45 ABC transporter transmembrane region
ABC_tran PF00005 325 - 473 2.3e-32 ABC transporter
SMC_N PF02463 338 - 513 5.5e-06 RecF/RecN/SMC N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1627
AccII CGCG 1 cut(s) 814
AciI CCGC 4 cut(s) 812, 814, 1250, 1354
AclWI GGATC 2 cut(s) 872, 1379
AcsI RAATTY 4 cut(s) 346, 920, 982, 1274
AcuI CTGAAG 2 cut(s) 545, 1314
AfaI GTAC 4 cut(s) 342, 523, 944, 1045
AfiI CCNNNNNNNGG 1 cut(s) 1085
AgsI TTSAA 5 cut(s) 225, 982, 988, 1132, 1303
AhlI ACTAGT 2 cut(s) 1057, 1156
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 1 cut(s) 717
AlwI GGATC 2 cut(s) 872, 1379
Ama87I CYCGRG 1 cut(s) 827
AoxI GGCC 4 cut(s) 673, 809, 1434, 1477
ApeKI GCWGC 4 cut(s) 287, 404, 530, 1259
ApoI RAATTY 4 cut(s) 346, 920, 982, 1274
ArsI GACNNNNNNTTYG 4 cut(s) 1067, 1099, 1224, 1256
AseI ATTAAT 1 cut(s) 203
Asp700I GAANNNNTTC 1 cut(s) 1030
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 809
AsuC2I CCSGG 1 cut(s) 1599
AsuHPI GGTGA 1 cut(s) 955
AvaI CYCGRG 1 cut(s) 827
BauI CACGAG 1 cut(s) 498
Bbv12I GWGCWC 1 cut(s) 316
BbvCI CCTCAGC 1 cut(s) 543
BbvI GCAGC 4 cut(s) 274, 416, 517, 1271
BccI CCATC 3 cut(s) 163, 1097, 1396
BcgI CGANNNNNNTGC 2 cut(s) 813, 847
BcnI CCSGG 1 cut(s) 1599
BcoDI GTCTC 1 cut(s) 717
BcuI ACTAGT 2 cut(s) 1057, 1156
BfmI CTRYAG 1 cut(s) 1628
BisI GCNGC 6 cut(s) 288, 405, 531, 812, 1251, 1260
BlsI GCNGC 6 cut(s) 289, 406, 532, 813, 1252, 1261
BmcAI AGTACT 1 cut(s) 1045
Bme1390I CCNGG 1 cut(s) 1599
BmeT110I CYCGRG 1 cut(s) 827
BmgT120I GGNCC 1 cut(s) 809
BmiI GGNNCC 1 cut(s) 810
BmrFI CCNGG 1 cut(s) 1599
BmsI GCATC 4 cut(s) 578, 695, 1235, 1513
BpmI CTGGAG 1 cut(s) 777
Bpu10I CCTNAGC 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 1293
BpuMI CCSGG 1 cut(s) 1599
BsaAI YACGTR 1 cut(s) 521
BsaJI CCNNGG 2 cut(s) 22, 812
BsaWI WCCGGW 1 cut(s) 620
Bsc4I CCNNNNNNNGG 1 cut(s) 1085
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 4 cut(s) 67, 134, 268, 1003
BseDI CCNNGG 2 cut(s) 22, 812
BseGI GGATG 2 cut(s) 256, 1226
BseLI CCNNNNNNNGG 1 cut(s) 1085
BseMII CTCAG 2 cut(s) 225, 534
BseNI ACTGG 4 cut(s) 67, 134, 268, 1003
BseRI GAGGAG 1 cut(s) 924
BseXI GCAGC 4 cut(s) 274, 416, 517, 1271
BsgI GTGCAG 2 cut(s) 744, 1349
Bsh1236I CGCG 1 cut(s) 814
Bsh1285I CGRYCG 1 cut(s) 1024
BshFI GGCC 4 cut(s) 675, 811, 1436, 1479
BsiEI CGRYCG 1 cut(s) 1024
BsiHKAI GWGCWC 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 827
BsiSI CCGG 3 cut(s) 120, 621, 1599
BslI CCNNNNNNNGG 1 cut(s) 1085
BsmAI GTCTC 1 cut(s) 717
BsmI GAATGC 1 cut(s) 1293
BsnI GGCC 4 cut(s) 675, 811, 1436, 1479
BsoBI CYCGRG 1 cut(s) 827
Bsp1286I GDGCHC 1 cut(s) 316
Bsp143I GATC 2 cut(s) 877, 1384
BspACI CCGC 4 cut(s) 812, 814, 1250, 1354
BspANI GGCC 4 cut(s) 675, 811, 1436, 1479
BspCNI CTCAG 2 cut(s) 224, 535
BspFNI CGCG 1 cut(s) 814
BspHI TCATGA 4 cut(s) 729, 1242, 1270, 1519
BspLI GGNNCC 1 cut(s) 810
BspPI GGATC 2 cut(s) 872, 1379
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 4 cut(s) 67, 134, 268, 1003
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 2 cut(s) 22, 812
BssMI GATC 2 cut(s) 877, 1384
BssSI CACGAG 1 cut(s) 498
BssT1I CCWWGG 1 cut(s) 22
Bst2BI CACGAG 1 cut(s) 498
Bst4CI ACNGT 6 cut(s) 130, 326, 526, 765, 1048, 1192
Bst6I CTCTTC 2 cut(s) 1034, 1634
BstAPI GCANNNNNTGC 1 cut(s) 1265
BstBAI YACGTR 1 cut(s) 521
BstDEI CTNAG 3 cut(s) 211, 453, 543
BstDSI CCRYGG 1 cut(s) 812
BstF5I GGATG 2 cut(s) 256, 1226
BstFNI CGCG 1 cut(s) 814
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 2 cut(s) 880, 1387
BstMAI GTCTC 1 cut(s) 717
BstMBI GATC 2 cut(s) 877, 1384
BstMCI CGRYCG 1 cut(s) 1024
BstMWI GCNNNNNNNGC 8 cut(s) 293, 503, 539, 583, 1256, 1259, 1265, 1351
BstSCI CCNGG 1 cut(s) 1597
BstSFI CTRYAG 1 cut(s) 1628
BstSNI TACGTA 1 cut(s) 521
BstUI CGCG 1 cut(s) 814
BstV1I GCAGC 4 cut(s) 274, 416, 517, 1271
BstX2I RGATCY 1 cut(s) 1384
BstXI CCANNNNNNTGG 1 cut(s) 1492
BstYI RGATCY 1 cut(s) 1384
BsuRI GGCC 4 cut(s) 675, 811, 1436, 1479
BtgI CCRYGG 1 cut(s) 812
BtsCI GGATG 2 cut(s) 256, 1226
BtsIMutI CAGTG 3 cut(s) 261, 269, 761
CciI TCATGA 4 cut(s) 729, 1242, 1270, 1519
CfoI GCGC 1 cut(s) 198
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 809
Cfr42I CCGCGG 1 cut(s) 815
Csp6I GTAC 4 cut(s) 341, 522, 943, 1044
CviAII CATG 7 cut(s) 103, 730, 1109, 1243, 1271, 1520, 1577
CviQI GTAC 4 cut(s) 341, 522, 943, 1044
DdeI CTNAG 3 cut(s) 211, 453, 543
DpnI GATC 2 cut(s) 879, 1386
DpnII GATC 2 cut(s) 877, 1384
Eam1104I CTCTTC 2 cut(s) 1034, 1634
EarI CTCTTC 2 cut(s) 1034, 1634
Eco105I TACGTA 1 cut(s) 521
Eco130I CCWWGG 1 cut(s) 22
Eco32I GATATC 2 cut(s) 961, 1114
Eco57I CTGAAG 2 cut(s) 545, 1314
Eco88I CYCGRG 1 cut(s) 827
EcoRI GAATTC 2 cut(s) 920, 1274
EcoRV GATATC 2 cut(s) 961, 1114
EcoT14I CCWWGG 1 cut(s) 22
EcoT22I ATGCAT 1 cut(s) 688
ErhI CCWWGG 1 cut(s) 22
FaeI CATG 7 cut(s) 106, 733, 1112, 1246, 1274, 1523, 1580
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FatI CATG 7 cut(s) 102, 729, 1108, 1242, 1270, 1519, 1576
FauI CCCGC 1 cut(s) 1347
FblI GTMKAC 1 cut(s) 1627
Fnu4HI GCNGC 6 cut(s) 288, 405, 531, 812, 1251, 1260
FokI GGATG 2 cut(s) 263, 1213
Fsp4HI GCNGC 6 cut(s) 288, 405, 531, 812, 1251, 1260
GlaI GCGC 1 cut(s) 197
GluI GCNGC 6 cut(s) 288, 405, 531, 812, 1251, 1260
GsuI CTGGAG 1 cut(s) 777
HaeIII GGCC 4 cut(s) 675, 811, 1436, 1479
HapII CCGG 3 cut(s) 120, 621, 1599
HhaI GCGC 1 cut(s) 198
Hin1II CATG 7 cut(s) 106, 733, 1112, 1246, 1274, 1523, 1580
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 4 cut(s) 88, 460, 996, 1512
HindII GTYRAC 4 cut(s) 88, 460, 996, 1512
HinfI GANTC 1 cut(s) 1006
HpaII CCGG 3 cut(s) 120, 621, 1599
HphI GGTGA 1 cut(s) 955
Hpy166II GTNNAC 7 cut(s) 88, 269, 460, 996, 1512, 1628, 1646
Hpy188I TCNGA 7 cut(s) 70, 308, 574, 908, 926, 958, 1231
Hpy188III TCNNGA 8 cut(s) 500, 730, 794, 1243, 1271, 1451, 1460, 1520
Hpy8I GTNNAC 7 cut(s) 88, 269, 460, 996, 1512, 1628, 1646
HpyAV CCTTC 4 cut(s) 391, 799, 1289, 1469
HpyCH4III ACNGT 6 cut(s) 130, 326, 526, 765, 1048, 1192
HpyCH4IV ACGT 1 cut(s) 520
HpyF10VI GCNNNNNNNGC 8 cut(s) 293, 503, 539, 583, 1256, 1259, 1265, 1351
HpyF3I CTNAG 3 cut(s) 211, 453, 543
HpySE526I ACGT 1 cut(s) 520
Hsp92II CATG 7 cut(s) 106, 733, 1112, 1246, 1274, 1523, 1580
HspAI GCGC 1 cut(s) 196
KspI CCGCGG 1 cut(s) 815
Kzo9I GATC 2 cut(s) 877, 1384
LmnI GCTCC 3 cut(s) 440, 796, 1088
Lsp1109I GCAGC 4 cut(s) 274, 416, 517, 1271
LweI GCATC 4 cut(s) 578, 695, 1235, 1513
MaeII ACGT 1 cut(s) 520
MaeIII GTNAC 5 cut(s) 94, 124, 611, 816, 1411
MalI GATC 2 cut(s) 879, 1386
MboI GATC 2 cut(s) 877, 1384
MboII GAAGA 5 cut(s) 596, 1051, 1156, 1388, 1651
MfeI CAATTG 1 cut(s) 507
MflI RGATCY 1 cut(s) 1384
MhlI GDGCHC 1 cut(s) 316
MlyI GAGTC 1 cut(s) 1015
MmeI TCCRAC 2 cut(s) 83, 418
Mph1103I ATGCAT 1 cut(s) 688
MroXI GAANNNNTTC 1 cut(s) 1030
MseI TTAA 5 cut(s) 203, 389, 1124, 1206, 1660
MslI CAYNNNNRTG 2 cut(s) 167, 873
MspA1I CMGCKG 3 cut(s) 407, 814, 1354
MspI CCGG 3 cut(s) 120, 621, 1599
MspR9I CCNGG 1 cut(s) 1599
MunI CAATTG 1 cut(s) 507
Mva1269I GAATGC 1 cut(s) 1293
MvnI CGCG 1 cut(s) 814
MwoI GCNNNNNNNGC 8 cut(s) 293, 503, 539, 583, 1256, 1259, 1265, 1351
NciI CCSGG 1 cut(s) 1599
NdeII GATC 2 cut(s) 877, 1384
NlaIII CATG 7 cut(s) 106, 733, 1112, 1246, 1274, 1523, 1580
NlaIV GGNNCC 1 cut(s) 810
NmeAIII GCCGAG 2 cut(s) 651, 1462
NmuCI GTSAC 2 cut(s) 611, 816
NsiI ATGCAT 1 cut(s) 688
PagI TCATGA 4 cut(s) 729, 1242, 1270, 1519
PctI GAATGC 1 cut(s) 1293
PdmI GAANNNNTTC 1 cut(s) 1030
PkrI GCNGC 6 cut(s) 289, 406, 532, 813, 1252, 1261
PleI GAGTC 1 cut(s) 1014
PpsI GAGTC 1 cut(s) 1014
Ppu21I YACGTR 1 cut(s) 521
PshBI ATTAAT 1 cut(s) 203
PspN4I GGNNCC 1 cut(s) 810
PspPI GGNCC 1 cut(s) 809
PsuI RGATCY 1 cut(s) 1384
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 4 cut(s) 342, 523, 944, 1045
RsaNI GTAC 4 cut(s) 341, 522, 943, 1044
RseI CAYNNNNRTG 2 cut(s) 167, 873
SacII CCGCGG 1 cut(s) 815
SaqAI TTAA 5 cut(s) 203, 389, 1124, 1206, 1660
SatI GCNGC 6 cut(s) 288, 405, 531, 812, 1251, 1260
Sau3AI GATC 2 cut(s) 877, 1384
Sau96I GGNCC 1 cut(s) 809
ScaI AGTACT 1 cut(s) 1045
SchI GAGTC 1 cut(s) 1015
ScrFI CCNGG 1 cut(s) 1599
SduI GDGCHC 1 cut(s) 316
SfaNI GCATC 4 cut(s) 578, 695, 1235, 1513
SfcI CTRYAG 1 cut(s) 1628
Sfr303I CCGCGG 1 cut(s) 815
SgrBI CCGCGG 1 cut(s) 815
SmiMI CAYNNNNRTG 2 cut(s) 167, 873
SmlI CTYRAG 1 cut(s) 1308
SmoI CTYRAG 1 cut(s) 1308
SnaBI TACGTA 1 cut(s) 521
SpeI ACTAGT 2 cut(s) 1057, 1156
SsiI CCGC 4 cut(s) 812, 814, 1250, 1354
StyD4I CCNGG 1 cut(s) 1597
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 6 cut(s) 130, 326, 526, 765, 1048, 1192
TaiI ACGT 1 cut(s) 523
TaqI TCGA 4 cut(s) 607, 833, 972, 1024
TaqII GACCGA 2 cut(s) 942, 1010
TatI WGTACW 2 cut(s) 340, 1043
TauI GCSGC 2 cut(s) 814, 1253
Tru1I TTAA 5 cut(s) 203, 389, 1124, 1206, 1660
Tru9I TTAA 5 cut(s) 203, 389, 1124, 1206, 1660
TscAI CASTG 3 cut(s) 268, 276, 768
TseFI GTSAC 2 cut(s) 611, 816
TseI GCWGC 4 cut(s) 287, 404, 530, 1259
Tsp45I GTSAC 2 cut(s) 611, 816
TspDTI ATGAA 6 cut(s) 642, 746, 1267, 1287, 1422, 1508
TspRI CASTG 3 cut(s) 268, 276, 768
VspI ATTAAT 1 cut(s) 203
XapI RAATTY 4 cut(s) 346, 920, 982, 1274
XbaI TCTAGA 1 cut(s) 1459
XcmI CCANNNNNNNNNTGG 1 cut(s) 889
XmiI GTMKAC 1 cut(s) 1627
XmnI GAANNNNTTC 1 cut(s) 1030
ZrmI AGTACT 1 cut(s) 1045
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.