Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0001991

Physical Location & Seq

Genomic Coordinates Reverse (-)

20737178 .. 20739827

Gene Structure 2650 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g16490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1653 bp

ATGTACGGCAGTTCACTGAAGCTCTTGGACAGCAAATGGTTCTCTACGGCGGCGAGCATATGGATACAGTGCACCAGCGGCTCCCTCTACACCTTCTCCGTCTACTCTCAGGCCATCAAGACAACGCAGGGCTTCGACCAGTCAACGCTCGACACCGTCTCCGTCTTCAAGGACTTCGGAGCCAATTGTGGCGTCCTCTCTGGCCTCCTCTACTCGCACTCCGCCAGGTGGCGAAAGGTCTGGTTGGTCCTCCTGGCCGGGGCGGCCCAGTGCTTTTTGGGTTATTTTCTAATGTGGGCGGCCGTCGCCGGCATTATTCCCCGGCCTCCGGTGCCGGTTATGTGTCTGTTCATGTTCGTGGCGGCTCATGCGGTGTCGTTCTTTAATACCTCTAATGTGGTCACTGGCGTCGGGAATTTTCCTTATTATAAGGGCACCATCGTTGGCATCATGAAGGGGTTTCTCGGTCTGAGTGGAGCAATCTTGATTCAAATATACCGGACATTTTTGCACAAGAAACCGGCTTCATTTCTTCTGATGCTGGCACTGCTACCTACCGTAAACACGTTGTTGCTTATGTGGTTTGTGAGAATCCACAGCAAGTGCGAAGGAGACGAGAAGAAGCACCTAAATAGTTTGTCGCTGATTGCGCTTGTTGTTGCGGTTTATCTTATGGGGTTTATAATTTTAGAGAACATCCTTACTTTGCGTTTTGCCATACGCTTCGTTGCTTTTGTTGCACTTCTGCTACTGCTTGCCTCACCTCTTTGCATTGCAATTAGAGCACATAAAAGGGAGTCCGATAGAGTTGCACGGACTTTATTAGTTGAGGGTGGCCAGTTAACAGATACCGAGGTCAACTGGCGAGATAGTGGGAAAAGGAATGAGCAAGATCGTTCAGGGTACCGTCATTTGCCTAGCAATACTGATCAAGAGTTGGATAGTAACGATGAAAGTACTTTGCAGTTGGGAGAAACTTTTAATCTACTCCAAGCTATGTGCACTATGGACTTCTGGATTTTGTTCATTGCCATGGCATTTGGAATGGGATCGGGACTTGCCACAGTGAATAACCTCAACCAGATAGGAAAATCGCTTGGTTACTCAATTTTTGAAACAGGTAGTTTGATTTCTTTGTGGAGCATCTGGAATTTCGTCGGGCGTTTTGGAGCTGGTTATGTATCAGATTATTTCCTGCACGCGAAAGGATTGGCAAGGCCATTGTTCATGGTCATCACTCTAGCAGGCATGAGCGTTGGTCATGCTGTGATTGCTTCCGGTATGCCTAGCGCTCTCTATGCTGGATCCTTACTGGTGGGCGTCTTTTATGGTTCTCAATGGTCACTAATGCCCACAATCGCTTCTGAAATATTCGGCGTGCTACATATGGGCACCATATTCAACGCCATTACCATAGCCGCGCCTGTGGGATCTTATATCTTTTCTGTGAGAGTGATCGGCCATATTTATGACAAAGAAGCGTCGGGAGCTTGGGATACATGCGCCGGAATTCGCTGCTTTAGGTTATCCTTCCTCATTATGGCAGGAGCCACCCTTTTCGGATCTCTCACTGCCTTAGCTTTGTTCTTTCGGACACGGAGTTTCTACAGTCAGGTAATCCTCCGAAGACCGCTATGTTATGTGCCGGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nodulin-like

Protein Analysis

551

Amino Acids

60.51

Weight (kDa)

9.13

Isoelectric Point (pI)

31.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nodulin-like	PF06813	12 - 260	1.3e-65	Nodulin-like
NFD4_C	PF23262	334 - 537	1.6e-20	NFD4, C-terminal domain
MFS_1	PF07690	341 - 534	4.4e-10	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001991)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18940 AT1G74780 AT2G34350 AT2G34350 AT2G34355 AT2G34355
fragaria_vesca	FvH4_5g15790 FvH4_5g15790 FvH4_5g15790 FvH4_5g15810
malus_domestica	MD00G1132000.v1.1 MD06G1185400.v1.1 MD06G1185500.v1.1 MD06G1185600.v1.1 MD14G1191700.v1.1
prunus_persica	Prupe.5G184300_v2.0.a1 Prupe.5G184300_v2.0.a1
pyrus_communis	pycom06g16490 pycom06g16500 pycom14g15940
rosa_chinensis	RchiOBHm_Chr7g0180321 RchiOBHm_Chr7g0180331
rosa_laevigata	RLG00000005276 RLG00000005277
rosa_multiflora	Rmu_sc0003526.1_g000013 Rmu_sc0003526.1_g000014 Rmu_sc0031066.1_g000002 Rmu_ssc0000099.1_g000002 Rmu_ssc0000099.1_g000004
rosa_roxburghii	Rroxscaffold_3G00272980 Rroxscaffold_3G00272990
rosa_rugosa	Rorug06G0432000 Rorug06G0432100 Rorug06G0432200 Rorug06G0432300
rosa_samantha	Rh7AG032600 Rh7AG032700 Rh7BG032600 Rh7BG032700 Rh7CG034500 Rh7CG034600 Rh7DG033200 Rh7DG033300
rosa_wichuraiana	Rw7G002720 Rw7G002730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	429, 683
Acc65I	GGTACC	1 cut(s)	903
AccB1I	GGYRCC	4 cut(s)	331, 434, 903, 1391
AccI	GTMKAC	1 cut(s)	102
AccII	CGCG	2 cut(s)	1202, 1421
AclWI	GGATC	5 cut(s)	1057, 1299, 1312, 1438, 1570
AcoI	YGGCCR	4 cut(s)	255, 300, 835, 1459
AcsI	RAATTY	3 cut(s)	415, 1150, 1509
AcuI	CTGAAG	1 cut(s)	38
AcyI	GRCGYC	3 cut(s)	192, 408, 1320
AfaI	GTAC	3 cut(s)	5, 905, 958
AfeI	AGCGCT	1 cut(s)	1291
AfiI	CCNNNNNNNGG	4 cut(s)	228, 259, 328, 1540
AflIII	ACRYGT	1 cut(s)	564
AgsI	TTSAA	4 cut(s)	169, 491, 1115, 1402
AjnI	CCWGG	2 cut(s)	224, 252
AluBI	AGCT	5 cut(s)	22, 995, 1172, 1490, 1580
AluI	AGCT	5 cut(s)	22, 995, 1172, 1490, 1580
Alw21I	GWGCWC	3 cut(s)	74, 787, 1004
Alw26I	GTCTC	2 cut(s)	163, 606
Alw44I	GTGCAC	2 cut(s)	70, 1000
AlwI	GGATC	5 cut(s)	1057, 1299, 1312, 1438, 1570
Aor51HI	AGCGCT	1 cut(s)	1291
AoxI	GGCC	9 cut(s)	111, 202, 255, 264, 300, 323, 835, 1217, 1459
ApaLI	GTGCAC	2 cut(s)	70, 1000
ApeKI	GCWGC	1 cut(s)	1515
ApoI	RAATTY	3 cut(s)	415, 1150, 1509
Asp718I	GGTACC	1 cut(s)	903
AspLEI	GCGC	4 cut(s)	652, 1292, 1423, 1505
AspS9I	GGNCC	2 cut(s)	247, 265
AsuC2I	CCSGG	2 cut(s)	259, 322
AsuHPI	GGTGA	1 cut(s)	753
AvaII	GGWCC	1 cut(s)	247
BaeGI	GKGCMC	4 cut(s)	74, 437, 1004, 1394
BaeI	ACNNNNGTAYC	2 cut(s)	840, 873
BalI	TGGCCA	1 cut(s)	837
BamHI	GGATCC	1 cut(s)	1304
BanI	GGYRCC	4 cut(s)	331, 434, 903, 1391
BbsI	GAAGAC	2 cut(s)	157, 1633
Bbv12I	GWGCWC	3 cut(s)	74, 787, 1004
BbvI	GCAGC	1 cut(s)	1502
BccI	CCATC	2 cut(s)	122, 446
BceAI	ACGGC	3 cut(s)	22, 63, 287
BcgI	CGANNNNNNTGC	2 cut(s)	791, 825
BciT130I	CCWGG	2 cut(s)	226, 254
BciVI	GTATCC	2 cut(s)	57, 1489
BclI	TGATCA	1 cut(s)	928
BcnI	CCSGG	2 cut(s)	259, 322
BcoDI	GTCTC	2 cut(s)	163, 606
BfaI	CTAG	4 cut(s)	918, 1241, 1287, 1651
BfmI	CTRYAG	1 cut(s)	1606
BfoI	RGCGCY	1 cut(s)	1293
BfuI	GTATCC	2 cut(s)	57, 1489
BglI	GCCNNNNNGGC	1 cut(s)	263
BisI	GCNGC	7 cut(s)	51, 79, 264, 300, 363, 1419, 1516
BlsI	GCNGC	7 cut(s)	52, 80, 265, 301, 364, 1420, 1517
BmcAI	AGTACT	1 cut(s)	958
Bme1390I	CCNGG	4 cut(s)	226, 254, 259, 322
Bme18I	GGWCC	1 cut(s)	247
BmgT120I	GGNCC	2 cut(s)	247, 265
BmiI	GGNNCC	8 cut(s)	82, 181, 333, 436, 905, 1306, 1393, 1549
BmrFI	CCNGG	4 cut(s)	226, 254, 259, 322
BmrI	ACTGGG	1 cut(s)	262
BmsI	GCATC	3 cut(s)	456, 528, 1152
BmuI	ACTGGG	1 cut(s)	262
BpiI	GAAGAC	2 cut(s)	157, 1633
Bpu10I	CCTNAGC	1 cut(s)	1576
BpuMI	CCSGG	2 cut(s)	259, 322
BsaHI	GRCGYC	3 cut(s)	192, 408, 1320
BsaJI	CCNNGG	4 cut(s)	258, 320, 852, 1032
BsaWI	WCCGGW	3 cut(s)	328, 498, 1277
BsaXI	ACNNNNNCTCC	6 cut(s)	80, 110, 143, 173, 203, 233
Bsc4I	CCNNNNNNNGG	4 cut(s)	228, 259, 328, 1540
Bse118I	RCCGGY	3 cut(s)	308, 334, 520
Bse1I	ACTGG	6 cut(s)	139, 268, 409, 838, 866, 1317
Bse3DI	GCAATG	2 cut(s)	771, 1026
BseBI	CCWGG	2 cut(s)	226, 254
BseDI	CCNNGG	4 cut(s)	258, 320, 852, 1032
BseGI	GGATG	1 cut(s)	696
BseLI	CCNNNNNNNGG	4 cut(s)	228, 259, 328, 1540
BseMI	GCAATG	2 cut(s)	771, 1026
BseMII	CTCAG	2 cut(s)	122, 461
BseNI	ACTGG	6 cut(s)	139, 268, 409, 838, 866, 1317
BseRI	GAGGAG	1 cut(s)	197
BseSI	GKGCMC	4 cut(s)	74, 437, 1004, 1394
BseX3I	CGGCCG	1 cut(s)	300
BseXI	GCAGC	1 cut(s)	1502
BsgI	GTGCAG	1 cut(s)	1181
Bsh1236I	CGCG	2 cut(s)	1202, 1421
Bsh1285I	CGRYCG	1 cut(s)	303
BshFI	GGCC	9 cut(s)	113, 204, 257, 266, 302, 325, 837, 1219, 1461
BshNI	GGYRCC	4 cut(s)	331, 434, 903, 1391
BsiEI	CGRYCG	1 cut(s)	303
BsiHKAI	GWGCWC	3 cut(s)	74, 787, 1004
BslFI	GGGAC	1 cut(s)	1068
BslI	CCNNNNNNNGG	4 cut(s)	228, 259, 328, 1540
BsmAI	GTCTC	2 cut(s)	163, 606
BsmBI	CGTCTC	2 cut(s)	163, 606
BsmFI	GGGAC	1 cut(s)	1068
BsnI	GGCC	9 cut(s)	113, 204, 257, 266, 302, 325, 837, 1219, 1461
Bsp1286I	GDGCHC	5 cut(s)	74, 437, 787, 1004, 1394
Bsp143I	GATC	7 cut(s)	892, 928, 1049, 1304, 1430, 1455, 1562
Bsp19I	CCATGG	1 cut(s)	1032
BspANI	GGCC	9 cut(s)	113, 204, 257, 266, 302, 325, 837, 1219, 1461
BspCNI	CTCAG	2 cut(s)	121, 462
BspFNI	CGCG	2 cut(s)	1202, 1421
BspHI	TCATGA	1 cut(s)	450
BspLI	GGNNCC	8 cut(s)	82, 181, 333, 436, 905, 1306, 1393, 1549
BspPI	GGATC	5 cut(s)	1057, 1299, 1312, 1438, 1570
BspT107I	GGYRCC	4 cut(s)	331, 434, 903, 1391
BsrDI	GCAATG	2 cut(s)	771, 1026
BsrFI	RCCGGY	3 cut(s)	308, 334, 520
BsrI	ACTGG	6 cut(s)	139, 268, 409, 838, 866, 1317
BssAI	RCCGGY	3 cut(s)	308, 334, 520
BssECI	CCNNGG	4 cut(s)	258, 320, 852, 1032
BssMI	GATC	7 cut(s)	892, 928, 1049, 1304, 1430, 1455, 1562
BssNI	GRCGYC	3 cut(s)	192, 408, 1320
BssT1I	CCWWGG	1 cut(s)	1032
Bst2UI	CCWGG	2 cut(s)	226, 254
Bst4CI	ACNGT	6 cut(s)	69, 157, 559, 908, 1066, 1610
BstACI	GRCGYC	3 cut(s)	192, 408, 1320
BstC8I	GCNNGC	7 cut(s)	55, 310, 543, 756, 1200, 1246, 1379
BstDEI	CTNAG	3 cut(s)	108, 470, 1576
BstDSI	CCRYGG	1 cut(s)	1032
BstF5I	GGATG	1 cut(s)	696
BstFNI	CGCG	2 cut(s)	1202, 1421
BstH2I	RGCGCY	1 cut(s)	1293
BstHHI	GCGC	4 cut(s)	652, 1292, 1423, 1505
BstKTI	GATC	7 cut(s)	895, 931, 1052, 1307, 1433, 1458, 1565
BstMAI	GTCTC	2 cut(s)	163, 606
BstMBI	GATC	7 cut(s)	892, 928, 1049, 1304, 1430, 1455, 1562
BstMCI	CGRYCG	1 cut(s)	303
BstNI	CCWGG	2 cut(s)	226, 254
BstNSI	RCATGY	1 cut(s)	1503
BstSCI	CCNGG	4 cut(s)	224, 252, 257, 320
BstSFI	CTRYAG	1 cut(s)	1606
BstSLI	GKGCMC	4 cut(s)	74, 437, 1004, 1394
BstUI	CGCG	2 cut(s)	1202, 1421
BstV1I	GCAGC	1 cut(s)	1502
BstV2I	GAAGAC	2 cut(s)	157, 1633
BstX2I	RGATCY	3 cut(s)	1304, 1430, 1562
BstYI	RGATCY	3 cut(s)	1304, 1430, 1562
BstZI	CGGCCG	1 cut(s)	300
BsuI	GTATCC	2 cut(s)	57, 1489
BsuRI	GGCC	9 cut(s)	113, 204, 257, 266, 302, 325, 837, 1219, 1461
BtgI	CCRYGG	1 cut(s)	1032
BtsCI	GGATG	1 cut(s)	696
BtsI	GCAGTG	2 cut(s)	545, 1569
BtsIMutI	CAGTG	7 cut(s)	14, 74, 275, 402, 545, 1071, 1569
Cac8I	GCNNGC	7 cut(s)	55, 310, 543, 756, 1200, 1246, 1379
CciI	TCATGA	1 cut(s)	450
CfoI	GCGC	4 cut(s)	652, 1292, 1423, 1505
Cfr10I	RCCGGY	3 cut(s)	308, 334, 520
Cfr13I	GGNCC	2 cut(s)	247, 265
CseI	GACGC	4 cut(s)	181, 397, 1309, 1470
Csp6I	GTAC	3 cut(s)	4, 904, 957
CviAII	CATG	8 cut(s)	352, 368, 451, 1033, 1228, 1249, 1262, 1500
CviQI	GTAC	3 cut(s)	4, 904, 957
DdeI	CTNAG	3 cut(s)	108, 470, 1576
DpnI	GATC	7 cut(s)	894, 930, 1051, 1306, 1432, 1457, 1564
DpnII	GATC	7 cut(s)	892, 928, 1049, 1304, 1430, 1455, 1562
EaeI	YGGCCR	4 cut(s)	255, 300, 835, 1459
EagI	CGGCCG	1 cut(s)	300
EciI	GGCGGA	1 cut(s)	211
EclXI	CGGCCG	1 cut(s)	300
Eco130I	CCWWGG	1 cut(s)	1032
Eco47I	GGWCC	1 cut(s)	247
Eco47III	AGCGCT	1 cut(s)	1291
Eco52I	CGGCCG	1 cut(s)	300
Eco57I	CTGAAG	1 cut(s)	38
EcoRI	GAATTC	1 cut(s)	1509
EcoRII	CCWGG	2 cut(s)	224, 252
EcoT14I	CCWWGG	1 cut(s)	1032
ErhI	CCWWGG	1 cut(s)	1032
Esp3I	CGTCTC	2 cut(s)	163, 606
FaeI	CATG	8 cut(s)	355, 371, 454, 1036, 1231, 1252, 1265, 1503
FaqI	GGGAC	1 cut(s)	1068
FatI	CATG	8 cut(s)	351, 367, 450, 1032, 1227, 1248, 1261, 1499
FauNDI	CATATG	2 cut(s)	59, 1386
FbaI	TGATCA	1 cut(s)	928
FblI	GTMKAC	1 cut(s)	102
Fnu4HI	GCNGC	7 cut(s)	51, 79, 264, 300, 363, 1419, 1516
FokI	GGATG	1 cut(s)	683
Fsp4HI	GCNGC	7 cut(s)	51, 79, 264, 300, 363, 1419, 1516
FspBI	CTAG	4 cut(s)	918, 1241, 1287, 1651
GlaI	GCGC	4 cut(s)	651, 1291, 1422, 1504
GluI	GCNGC	7 cut(s)	51, 79, 264, 300, 363, 1419, 1516
HaeII	RGCGCY	1 cut(s)	1293
HaeIII	GGCC	9 cut(s)	113, 204, 257, 266, 302, 325, 837, 1219, 1461
HgaI	GACGC	4 cut(s)	181, 397, 1309, 1470
HhaI	GCGC	4 cut(s)	652, 1292, 1423, 1505
Hin1I	GRCGYC	3 cut(s)	192, 408, 1320
Hin1II	CATG	8 cut(s)	355, 371, 454, 1036, 1231, 1252, 1265, 1503
Hin6I	GCGC	4 cut(s)	650, 1290, 1421, 1503
HinP1I	GCGC	4 cut(s)	650, 1290, 1421, 1503
HincII	GTYRAC	3 cut(s)	144, 843, 859
HindII	GTYRAC	3 cut(s)	144, 843, 859
HinfI	GANTC	3 cut(s)	487, 591, 797
HpaI	GTTAAC	1 cut(s)	843
HphI	GGTGA	1 cut(s)	753
Hpy166II	GTNNAC	8 cut(s)	14, 72, 103, 144, 562, 843, 859, 1002
Hpy188I	TCNGA	9 cut(s)	179, 471, 537, 802, 1186, 1366, 1562, 1593, 1625
Hpy188III	TCNNGA	9 cut(s)	118, 412, 451, 484, 932, 1015, 1053, 1147, 1485
Hpy8I	GTNNAC	8 cut(s)	14, 72, 103, 144, 562, 843, 859, 1002
Hpy99I	CGWCG	4 cut(s)	308, 413, 1160, 1486
HpyAV	CCTTC	4 cut(s)	103, 448, 602, 1540
HpyCH4III	ACNGT	6 cut(s)	69, 157, 559, 908, 1066, 1610
HpyCH4IV	ACGT	1 cut(s)	566
HpyCH4V	TGCA	9 cut(s)	72, 511, 740, 771, 776, 812, 964, 1002, 1198
HpyF3I	CTNAG	3 cut(s)	108, 470, 1576
HpySE526I	ACGT	1 cut(s)	566
Hsp92I	GRCGYC	3 cut(s)	192, 408, 1320
Hsp92II	CATG	8 cut(s)	355, 371, 454, 1036, 1231, 1252, 1265, 1503
HspAI	GCGC	4 cut(s)	650, 1290, 1421, 1503
KpnI	GGTACC	1 cut(s)	907
KroI	GCCGGC	1 cut(s)	308
KroNI	GCCGGC	1 cut(s)	310
Ksp22I	TGATCA	1 cut(s)	928
KspAI	GTTAAC	1 cut(s)	843
Kzo9I	GATC	7 cut(s)	892, 928, 1049, 1304, 1430, 1455, 1562
LmnI	GCTCC	7 cut(s)	86, 179, 476, 1140, 1169, 1487, 1547
Lsp1109I	GCAGC	1 cut(s)	1502
LweI	GCATC	3 cut(s)	456, 528, 1152
MaeI	CTAG	4 cut(s)	918, 1241, 1287, 1651
MaeII	ACGT	1 cut(s)	566
MaeIII	GTNAC	4 cut(s)	400, 944, 1100, 1341
MalI	GATC	7 cut(s)	894, 930, 1051, 1306, 1432, 1457, 1564
MboI	GATC	7 cut(s)	892, 928, 1049, 1304, 1430, 1455, 1562
MboII	GAAGA	4 cut(s)	157, 524, 631, 1638
MfeI	CAATTG	1 cut(s)	184
MflI	RGATCY	3 cut(s)	1304, 1430, 1562
MhlI	GDGCHC	5 cut(s)	74, 437, 787, 1004, 1394
MlsI	TGGCCA	1 cut(s)	837
MluCI	AATT	7 cut(s)	184, 415, 684, 777, 1107, 1150, 1509
MluNI	TGGCCA	1 cut(s)	837
MlyI	GAGTC	1 cut(s)	806
MmeI	TCCRAC	1 cut(s)	918
Mox20I	TGGCCA	1 cut(s)	837
MroNI	GCCGGC	1 cut(s)	308
MscI	TGGCCA	1 cut(s)	837
MseI	TTAA	3 cut(s)	384, 842, 981
MslI	CAYNNNNRTG	3 cut(s)	356, 1031, 1467
Msp20I	TGGCCA	1 cut(s)	837
MspA1I	CMGCKG	1 cut(s)	78
MspR9I	CCNGG	4 cut(s)	226, 254, 259, 322
MunI	CAATTG	1 cut(s)	184
MvaI	CCWGG	2 cut(s)	226, 254
MvnI	CGCG	2 cut(s)	1202, 1421
NaeI	GCCGGC	1 cut(s)	310
NciI	CCSGG	2 cut(s)	259, 322
NcoI	CCATGG	1 cut(s)	1032
NdeI	CATATG	2 cut(s)	59, 1386
NdeII	GATC	7 cut(s)	892, 928, 1049, 1304, 1430, 1455, 1562
NgoMIV	GCCGGC	1 cut(s)	308
NlaIII	CATG	8 cut(s)	355, 371, 454, 1036, 1231, 1252, 1265, 1503
NlaIV	GGNNCC	8 cut(s)	82, 181, 333, 436, 905, 1306, 1393, 1549
NmuCI	GTSAC	2 cut(s)	400, 1341
NspI	RCATGY	1 cut(s)	1503
PagI	TCATGA	1 cut(s)	450
PdiI	GCCGGC	1 cut(s)	310
PfeI	GAWTC	2 cut(s)	487, 591
PflFI	GACNNNGTC	1 cut(s)	155
PkrI	GCNGC	7 cut(s)	52, 80, 265, 301, 364, 1420, 1517
PleI	GAGTC	1 cut(s)	805
PpsI	GAGTC	1 cut(s)	805
PsiI	TTATAA	2 cut(s)	429, 683
Psp6I	CCWGG	2 cut(s)	224, 252
PspGI	CCWGG	2 cut(s)	224, 252
PspN4I	GGNNCC	8 cut(s)	82, 181, 333, 436, 905, 1306, 1393, 1549
PspPI	GGNCC	2 cut(s)	247, 265
PsuI	RGATCY	3 cut(s)	1304, 1430, 1562
PsyI	GACNNNGTC	1 cut(s)	155
RsaI	GTAC	3 cut(s)	5, 905, 958
RsaNI	GTAC	3 cut(s)	4, 904, 957
RseI	CAYNNNNRTG	3 cut(s)	356, 1031, 1467
SaqAI	TTAA	3 cut(s)	384, 842, 981
SatI	GCNGC	7 cut(s)	51, 79, 264, 300, 363, 1419, 1516
Sau3AI	GATC	7 cut(s)	892, 928, 1049, 1304, 1430, 1455, 1562
Sau96I	GGNCC	2 cut(s)	247, 265
ScaI	AGTACT	1 cut(s)	958
SchI	GAGTC	1 cut(s)	806
ScrFI	CCNGG	4 cut(s)	226, 254, 259, 322
SduI	GDGCHC	5 cut(s)	74, 437, 787, 1004, 1394
SfaNI	GCATC	3 cut(s)	456, 528, 1152
SfcI	CTRYAG	1 cut(s)	1606
SfiI	GGCCNNNNNGGCC	1 cut(s)	263
SinI	GGWCC	1 cut(s)	247
SmiMI	CAYNNNNRTG	3 cut(s)	356, 1031, 1467
Sse9I	AATT	7 cut(s)	184, 415, 684, 777, 1107, 1150, 1509
SspI	AATATT	1 cut(s)	1371
SspMI	CTAG	4 cut(s)	918, 1241, 1287, 1651
StyD4I	CCNGG	4 cut(s)	224, 252, 257, 320
StyI	CCWWGG	1 cut(s)	1032
TaaI	ACNGT	6 cut(s)	69, 157, 559, 908, 1066, 1610
TaiI	ACGT	1 cut(s)	569
TaqI	TCGA	2 cut(s)	135, 150
TaqII	GACCGA	1 cut(s)	455
TasI	AATT	7 cut(s)	184, 415, 684, 777, 1107, 1150, 1509
TatI	WGTACW	1 cut(s)	956
TauI	GCSGC	6 cut(s)	53, 81, 266, 302, 365, 1421
TfiI	GAWTC	2 cut(s)	487, 591
Tru1I	TTAA	3 cut(s)	384, 842, 981
Tru9I	TTAA	3 cut(s)	384, 842, 981
TscAI	CASTG	7 cut(s)	21, 74, 275, 409, 552, 1071, 1576
TseFI	GTSAC	2 cut(s)	400, 1341
TseI	GCWGC	1 cut(s)	1515
Tsp45I	GTSAC	2 cut(s)	400, 1341
TspDTI	ATGAA	6 cut(s)	340, 467, 516, 966, 1015, 1216
TspGWI	ACGGA	4 cut(s)	88, 151, 829, 1612
TspRI	CASTG	7 cut(s)	21, 74, 275, 409, 552, 1071, 1576
Tth111I	GACNNNGTC	1 cut(s)	155
VneI	GTGCAC	2 cut(s)	70, 1000
VpaK11BI	GGWCC	1 cut(s)	247
XapI	RAATTY	3 cut(s)	415, 1150, 1509
XceI	RCATGY	1 cut(s)	1503
XmiI	GTMKAC	1 cut(s)	102
XspI	CTAG	4 cut(s)	918, 1241, 1287, 1651
ZrmI	AGTACT	1 cut(s)	958

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.