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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 63 |
| AclWI | GGATC | 1 cut(s) | 310 |
| AcuI | CTGAAG | 2 cut(s) | 303, 417 |
| AgsI | TTSAA | 1 cut(s) | 235 |
| AluBI | AGCT | 3 cut(s) | 86, 392, 401 |
| AluI | AGCT | 3 cut(s) | 86, 392, 401 |
| Alw26I | GTCTC | 1 cut(s) | 379 |
| AlwI | GGATC | 1 cut(s) | 310 |
| Ama87I | CYCGRG | 1 cut(s) | 45 |
| ApeKI | GCWGC | 1 cut(s) | 392 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 78 |
| AsuHPI | GGTGA | 1 cut(s) | 77 |
| AsuII | TTCGAA | 1 cut(s) | 132 |
| AvaI | CYCGRG | 1 cut(s) | 45 |
| BauI | CACGAG | 1 cut(s) | 328 |
| BbsI | GAAGAC | 1 cut(s) | 109 |
| BbvI | GCAGC | 1 cut(s) | 379 |
| BccI | CCATC | 2 cut(s) | 157, 203 |
| BcoDI | GTCTC | 1 cut(s) | 379 |
| BisI | GCNGC | 1 cut(s) | 393 |
| BlsI | GCNGC | 1 cut(s) | 394 |
| BmeT110I | CYCGRG | 1 cut(s) | 45 |
| BmsI | GCATC | 1 cut(s) | 47 |
| BpiI | GAAGAC | 1 cut(s) | 109 |
| Bpu14I | TTCGAA | 1 cut(s) | 132 |
| BsaBI | GATNNNNATC | 1 cut(s) | 337 |
| Bse8I | GATNNNNATC | 1 cut(s) | 337 |
| BseJI | GATNNNNATC | 1 cut(s) | 337 |
| BseXI | GCAGC | 1 cut(s) | 379 |
| BsiHKCI | CYCGRG | 1 cut(s) | 45 |
| BsiSI | CCGG | 1 cut(s) | 367 |
| BsmAI | GTCTC | 1 cut(s) | 379 |
| BsmBI | CGTCTC | 1 cut(s) | 379 |
| BsmI | GAATGC | 1 cut(s) | 40 |
| BsoBI | CYCGRG | 1 cut(s) | 45 |
| Bsp119I | TTCGAA | 1 cut(s) | 132 |
| Bsp143I | GATC | 4 cut(s) | 52, 274, 302, 332 |
| BspACI | CCGC | 1 cut(s) | 63 |
| BspPI | GGATC | 1 cut(s) | 310 |
| BspT104I | TTCGAA | 1 cut(s) | 132 |
| BssMI | GATC | 4 cut(s) | 52, 274, 302, 332 |
| BssSI | CACGAG | 1 cut(s) | 328 |
| Bst2BI | CACGAG | 1 cut(s) | 328 |
| Bst6I | CTCTTC | 5 cut(s) | 91, 129, 153, 192, 282 |
| BstBI | TTCGAA | 1 cut(s) | 132 |
| BstC8I | GCNNGC | 1 cut(s) | 380 |
| BstKTI | GATC | 4 cut(s) | 55, 277, 305, 335 |
| BstMAI | GTCTC | 1 cut(s) | 379 |
| BstMBI | GATC | 4 cut(s) | 52, 274, 302, 332 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 398 |
| BstV1I | GCAGC | 1 cut(s) | 379 |
| BstV2I | GAAGAC | 1 cut(s) | 109 |
| Cac8I | GCNNGC | 1 cut(s) | 380 |
| CviJI | RGCY | 6 cut(s) | 86, 115, 366, 382, 392, 401 |
| CviKI_1 | RGCY | 6 cut(s) | 86, 115, 366, 382, 392, 401 |
| DpnI | GATC | 4 cut(s) | 54, 276, 304, 334 |
| DpnII | GATC | 4 cut(s) | 52, 274, 302, 332 |
| Eam1104I | CTCTTC | 5 cut(s) | 91, 129, 153, 192, 282 |
| EarI | CTCTTC | 5 cut(s) | 91, 129, 153, 192, 282 |
| Eco57I | CTGAAG | 2 cut(s) | 303, 417 |
| Eco88I | CYCGRG | 1 cut(s) | 45 |
| EcoT22I | ATGCAT | 1 cut(s) | 40 |
| Esp3I | CGTCTC | 1 cut(s) | 379 |
| Fnu4HI | GCNGC | 1 cut(s) | 393 |
| Fsp4HI | GCNGC | 1 cut(s) | 393 |
| GluI | GCNGC | 1 cut(s) | 393 |
| HapII | CCGG | 1 cut(s) | 367 |
| HinfI | GANTC | 2 cut(s) | 79, 323 |
| HpaII | CCGG | 1 cut(s) | 367 |
| HphI | GGTGA | 1 cut(s) | 77 |
| Hpy188I | TCNGA | 1 cut(s) | 279 |
| Hpy188III | TCNNGA | 1 cut(s) | 47 |
| HpyAV | CCTTC | 1 cut(s) | 229 |
| HpyCH4V | TGCA | 1 cut(s) | 38 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 398 |
| Kzo9I | GATC | 4 cut(s) | 52, 274, 302, 332 |
| LpnPI | CCDG | 3 cut(s) | 76, 96, 380 |
| Lsp1109I | GCAGC | 1 cut(s) | 379 |
| LweI | GCATC | 1 cut(s) | 47 |
| MaeIII | GTNAC | 2 cut(s) | 217, 413 |
| MalI | GATC | 4 cut(s) | 54, 276, 304, 334 |
| MboI | GATC | 4 cut(s) | 52, 274, 302, 332 |
| MnlI | CCTC | 5 cut(s) | 8, 92, 130, 240, 363 |
| Mph1103I | ATGCAT | 1 cut(s) | 40 |
| MroXI | GAANNNNTTC | 1 cut(s) | 78 |
| MspI | CCGG | 1 cut(s) | 367 |
| Mva1269I | GAATGC | 1 cut(s) | 40 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 398 |
| NdeII | GATC | 4 cut(s) | 52, 274, 302, 332 |
| NmeAIII | GCCGAG | 1 cut(s) | 408 |
| NsiI | ATGCAT | 1 cut(s) | 40 |
| NspV | TTCGAA | 1 cut(s) | 132 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 52 |
| PctI | GAATGC | 1 cut(s) | 40 |
| PdmI | GAANNNNTTC | 1 cut(s) | 78 |
| PfeI | GAWTC | 2 cut(s) | 79, 323 |
| PkrI | GCNGC | 1 cut(s) | 394 |
| SatI | GCNGC | 1 cut(s) | 393 |
| Sau3AI | GATC | 4 cut(s) | 52, 274, 302, 332 |
| SetI | ASST | 4 cut(s) | 13, 88, 394, 403 |
| SfaNI | GCATC | 1 cut(s) | 47 |
| SfuI | TTCGAA | 1 cut(s) | 132 |
| SsiI | CCGC | 1 cut(s) | 63 |
| TaqI | TCGA | 3 cut(s) | 132, 305, 335 |
| TfiI | GAWTC | 2 cut(s) | 79, 323 |
| TseI | GCWGC | 1 cut(s) | 392 |
| TspDTI | ATGAA | 5 cut(s) | 84, 134, 171, 204, 210 |
| XmnI | GAANNNNTTC | 1 cut(s) | 78 |
| Zsp2I | ATGCAT | 1 cut(s) | 40 |