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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 63 |
| AciI | CCGC | 1 cut(s) | 63 |
| AcuI | CTGAAG | 2 cut(s) | 300, 417 |
| AgsI | TTSAA | 1 cut(s) | 410 |
| AluBI | AGCT | 4 cut(s) | 86, 148, 392, 401 |
| AluI | AGCT | 4 cut(s) | 86, 148, 392, 401 |
| AoxI | GGCC | 1 cut(s) | 104 |
| ApeKI | GCWGC | 1 cut(s) | 392 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 78 |
| AsuHPI | GGTGA | 1 cut(s) | 130 |
| BbsI | GAAGAC | 2 cut(s) | 164, 180 |
| BbvI | GCAGC | 1 cut(s) | 379 |
| BfaI | CTAG | 2 cut(s) | 45, 149 |
| BisI | GCNGC | 2 cut(s) | 64, 393 |
| BlsI | GCNGC | 2 cut(s) | 65, 394 |
| BpiI | GAAGAC | 2 cut(s) | 164, 180 |
| Bse118I | RCCGGY | 1 cut(s) | 115 |
| Bse1I | ACTGG | 1 cut(s) | 251 |
| BseGI | GGATG | 1 cut(s) | 187 |
| BseNI | ACTGG | 1 cut(s) | 251 |
| BseXI | GCAGC | 1 cut(s) | 379 |
| BshFI | GGCC | 1 cut(s) | 106 |
| BsiSI | CCGG | 1 cut(s) | 116 |
| BsmI | GAATGC | 1 cut(s) | 40 |
| BsnI | GGCC | 1 cut(s) | 106 |
| Bsp143I | GATC | 1 cut(s) | 52 |
| BspACI | CCGC | 1 cut(s) | 63 |
| BspANI | GGCC | 1 cut(s) | 106 |
| BspQI | GCTCTTC | 1 cut(s) | 138 |
| BsrBI | CCGCTC | 1 cut(s) | 63 |
| BsrFI | RCCGGY | 1 cut(s) | 115 |
| BsrI | ACTGG | 1 cut(s) | 251 |
| BssAI | RCCGGY | 1 cut(s) | 115 |
| BssMI | GATC | 1 cut(s) | 52 |
| Bst6I | CTCTTC | 3 cut(s) | 138, 233, 279 |
| BstF5I | GGATG | 1 cut(s) | 187 |
| BstKTI | GATC | 1 cut(s) | 55 |
| BstMBI | GATC | 1 cut(s) | 52 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 112, 398 |
| BstV1I | GCAGC | 1 cut(s) | 379 |
| BstV2I | GAAGAC | 2 cut(s) | 164, 180 |
| BsuRI | GGCC | 1 cut(s) | 106 |
| BtsCI | GGATG | 1 cut(s) | 187 |
| BtsIMutI | CAGTG | 1 cut(s) | 258 |
| Cfr10I | RCCGGY | 1 cut(s) | 115 |
| CviJI | RGCY | 6 cut(s) | 86, 106, 115, 148, 392, 401 |
| CviKI_1 | RGCY | 6 cut(s) | 86, 106, 115, 148, 392, 401 |
| DpnI | GATC | 1 cut(s) | 54 |
| DpnII | GATC | 1 cut(s) | 52 |
| Eam1104I | CTCTTC | 3 cut(s) | 138, 233, 279 |
| EarI | CTCTTC | 3 cut(s) | 138, 233, 279 |
| Eco57I | CTGAAG | 2 cut(s) | 300, 417 |
| FaiI | YATR | 2 cut(s) | 275, 326 |
| Fnu4HI | GCNGC | 2 cut(s) | 64, 393 |
| FokI | GGATG | 1 cut(s) | 194 |
| Fsp4HI | GCNGC | 2 cut(s) | 64, 393 |
| FspBI | CTAG | 2 cut(s) | 45, 149 |
| GluI | GCNGC | 2 cut(s) | 64, 393 |
| HaeIII | GGCC | 1 cut(s) | 106 |
| HapII | CCGG | 1 cut(s) | 116 |
| HinfI | GANTC | 3 cut(s) | 79, 257, 320 |
| HpaII | CCGG | 1 cut(s) | 116 |
| HphI | GGTGA | 1 cut(s) | 130 |
| Hpy99I | CGWCG | 1 cut(s) | 340 |
| HpyAV | CCTTC | 1 cut(s) | 363 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 112, 398 |
| Kzo9I | GATC | 1 cut(s) | 52 |
| LguI | GCTCTTC | 1 cut(s) | 138 |
| LmnI | GCTCC | 2 cut(s) | 60, 363 |
| LpnPI | CCDG | 3 cut(s) | 96, 129, 264 |
| Lsp1109I | GCAGC | 1 cut(s) | 379 |
| MaeI | CTAG | 2 cut(s) | 45, 149 |
| MaeIII | GTNAC | 2 cut(s) | 202, 220 |
| MalI | GATC | 1 cut(s) | 54 |
| MbiI | CCGCTC | 1 cut(s) | 63 |
| MboI | GATC | 1 cut(s) | 52 |
| MlyI | GAGTC | 1 cut(s) | 266 |
| MnlI | CCTC | 9 cut(s) | 8, 21, 50, 61, 92, 96, 160, 228, 234 |
| MroXI | GAANNNNTTC | 1 cut(s) | 78 |
| MspI | CCGG | 1 cut(s) | 116 |
| Mva1269I | GAATGC | 1 cut(s) | 40 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 112, 398 |
| NdeII | GATC | 1 cut(s) | 52 |
| NmuCI | GTSAC | 1 cut(s) | 220 |
| PciSI | GCTCTTC | 1 cut(s) | 138 |
| PctI | GAATGC | 1 cut(s) | 40 |
| PdmI | GAANNNNTTC | 1 cut(s) | 78 |
| PfeI | GAWTC | 2 cut(s) | 79, 320 |
| PkrI | GCNGC | 2 cut(s) | 65, 394 |
| PleI | GAGTC | 1 cut(s) | 265 |
| PpsI | GAGTC | 1 cut(s) | 265 |
| SapI | GCTCTTC | 1 cut(s) | 138 |
| SatI | GCNGC | 2 cut(s) | 64, 393 |
| Sau3AI | GATC | 1 cut(s) | 52 |
| SchI | GAGTC | 1 cut(s) | 266 |
| SetI | ASST | 5 cut(s) | 13, 88, 150, 394, 403 |
| SsiI | CCGC | 1 cut(s) | 63 |
| SspMI | CTAG | 2 cut(s) | 45, 149 |
| TaqI | TCGA | 1 cut(s) | 55 |
| TauI | GCSGC | 1 cut(s) | 66 |
| TfiI | GAWTC | 2 cut(s) | 79, 320 |
| TscAI | CASTG | 1 cut(s) | 258 |
| TseFI | GTSAC | 1 cut(s) | 220 |
| TseI | GCWGC | 1 cut(s) | 392 |
| Tsp45I | GTSAC | 1 cut(s) | 220 |
| TspDTI | ATGAA | 2 cut(s) | 141, 341 |
| TspRI | CASTG | 1 cut(s) | 258 |
| XmnI | GAANNNNTTC | 1 cut(s) | 78 |
| XspI | CTAG | 2 cut(s) | 45, 149 |