FvH4_3g43480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001497
Physical Location & Seq
Forward (+)
36222650 .. 36226598
3949 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43480.t1

Sequence Viewer

Length: 561 bp
ATGGGGAGAGGTAGGGGAAAGGGAAAGAGGTTAACTGTCACCAATCATGATGATCCTGGAAGCGGCGAGGAAGAGAAAATACCCGCACAAAAGAGAAGAGGAAGACCATTAAAGCCTTTAAAGGATGAAATTGATGAAGAAGAAGTAGAAAAAGTAGAATCAGATGACATTGAGAATGCAAAAGCTGGTATCACAAGCAAAGAAATAAAAATCCCCACTGCAGCAGAGAATGGAAAGAAGAGGAAGCGGAACTCACAGGCCAAGGAGAAGTCAGATTCAGTGAAGGAGGAAAACGGTAATGGAACACGATCAAGTACAGATGACTCAACTAAATCTAATGGATTTCGGCATAATGGGAGCAGGCGGAAAAATAAACCCCGTCGAGCTGCTGAAGCTGGGGTTGAATGCAAATGTGGGTTCATGGTAGTGGTTGATATTGTTGAAGACAAAATGAAGAACTTTGATATTTCTAGTATAAACACGAAAAGAAAGGTGATTGATATTTCAACAATGGTACTAATAGTTATTGCTGAAGATGGCAGTGCTGCATTCCTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

20.53

Weight (kDa)

9.26

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 63, 84, 247, 364
AclWI GGATC 1 cut(s) 47
AcuI CTGAAG 2 cut(s) 411, 552
AfaI GTAC 2 cut(s) 316, 516
AfiI CCNNNNNNNGG 1 cut(s) 62
AgsI TTSAA 3 cut(s) 404, 443, 507
AjnI CCWGG 1 cut(s) 55
AluBI AGCT 3 cut(s) 185, 386, 395
AluI AGCT 3 cut(s) 185, 386, 395
AlwI GGATC 1 cut(s) 47
AoxI GGCC 1 cut(s) 258
ApeKI GCWGC 3 cut(s) 221, 386, 545
AsuHPI GGTGA 2 cut(s) 31, 505
BarI GAAGNNNNNNTAC 2 cut(s) 63, 95
BbsI GAAGAC 2 cut(s) 109, 450
BbvI GCAGC 3 cut(s) 233, 373, 532
BccI CCATC 1 cut(s) 530
BciT130I CCWGG 1 cut(s) 57
BfaI CTAG 1 cut(s) 471
BfmI CTRYAG 1 cut(s) 219
BisI GCNGC 4 cut(s) 64, 222, 387, 546
BlsI GCNGC 4 cut(s) 65, 223, 388, 547
Bme1390I CCNGG 1 cut(s) 57
BmrFI CCNGG 1 cut(s) 57
BpiI GAAGAC 2 cut(s) 109, 450
BsaJI CCNNGG 1 cut(s) 261
Bsc4I CCNNNNNNNGG 1 cut(s) 62
BseBI CCWGG 1 cut(s) 57
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 1 cut(s) 130
BseLI CCNNNNNNNGG 1 cut(s) 62
BseXI GCAGC 3 cut(s) 233, 373, 532
BseYI CCCAGC 1 cut(s) 395
BshFI GGCC 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 62
BsmI GAATGC 3 cut(s) 181, 410, 548
BsnI GGCC 1 cut(s) 260
Bsp143I GATC 2 cut(s) 52, 308
BspACI CCGC 4 cut(s) 63, 84, 247, 364
BspANI GGCC 1 cut(s) 260
BspHI TCATGA 1 cut(s) 46
BspMAI CTGCAG 1 cut(s) 223
BspPI GGATC 1 cut(s) 47
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 2 cut(s) 52, 308
BssT1I CCWWGG 1 cut(s) 261
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 2 cut(s) 37, 296
Bst6I CTCTTC 3 cut(s) 66, 91, 233
BstC8I GCNNGC 1 cut(s) 362
BstF5I GGATG 1 cut(s) 130
BstKTI GATC 2 cut(s) 55, 311
BstMBI GATC 2 cut(s) 52, 308
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 1 cut(s) 57
BstSCI CCNGG 1 cut(s) 55
BstSFI CTRYAG 1 cut(s) 219
BstV1I GCAGC 3 cut(s) 233, 373, 532
BstV2I GAAGAC 2 cut(s) 109, 450
BsuRI GGCC 1 cut(s) 260
BtsCI GGATG 1 cut(s) 130
BtsI GCAGTG 2 cut(s) 216, 547
BtsIMutI CAGTG 3 cut(s) 216, 285, 547
Cac8I GCNNGC 1 cut(s) 362
CciI TCATGA 1 cut(s) 46
Csp6I GTAC 2 cut(s) 315, 515
CviAII CATG 2 cut(s) 47, 421
CviJI RGCY 5 cut(s) 115, 185, 260, 386, 395
CviKI_1 RGCY 5 cut(s) 115, 185, 260, 386, 395
CviQI GTAC 2 cut(s) 315, 515
DpnI GATC 2 cut(s) 54, 310
DpnII GATC 2 cut(s) 52, 308
DraI TTTAAA 1 cut(s) 120
Eam1104I CTCTTC 3 cut(s) 66, 91, 233
EarI CTCTTC 3 cut(s) 66, 91, 233
EciI GGCGGA 1 cut(s) 379
Eco130I CCWWGG 1 cut(s) 261
Eco57I CTGAAG 2 cut(s) 411, 552
EcoRII CCWGG 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 2 cut(s) 50, 424
FaiI YATR 4 cut(s) 48, 351, 422, 476
FatI CATG 2 cut(s) 46, 420
FauI CCCGC 1 cut(s) 91
Fnu4HI GCNGC 4 cut(s) 64, 222, 387, 546
FokI GGATG 1 cut(s) 137
Fsp4HI GCNGC 4 cut(s) 64, 222, 387, 546
FspBI CTAG 1 cut(s) 471
GluI GCNGC 4 cut(s) 64, 222, 387, 546
GsaI CCCAGC 1 cut(s) 399
HaeIII GGCC 1 cut(s) 260
Hin1II CATG 2 cut(s) 50, 424
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HinfI GANTC 3 cut(s) 158, 275, 323
HpaI GTTAAC 1 cut(s) 33
HphI GGTGA 2 cut(s) 31, 505
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 2 cut(s) 163, 274
Hpy188III TCNNGA 2 cut(s) 47, 553
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 1 cut(s) 384
HpyAV CCTTC 1 cut(s) 277
HpyCH4III ACNGT 2 cut(s) 37, 296
HpyCH4V TGCA 4 cut(s) 179, 221, 408, 548
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
Hsp92II CATG 2 cut(s) 50, 424
KspAI GTTAAC 1 cut(s) 33
Kzo9I GATC 2 cut(s) 52, 308
LmnI GCTCC 1 cut(s) 357
LpnPI CCDG 6 cut(s) 42, 69, 171, 242, 346, 381
Lsp1109I GCAGC 3 cut(s) 233, 373, 532
MaeI CTAG 1 cut(s) 471
MaeIII GTNAC 1 cut(s) 37
MalI GATC 2 cut(s) 54, 310
MboI GATC 2 cut(s) 52, 308
MboII GAAGA 9 cut(s) 83, 108, 114, 149, 152, 250, 455, 466, 545
MluCI AATT 1 cut(s) 129
MlyI GAGTC 1 cut(s) 317
MnlI CCTC 5 cut(s) 21, 61, 92, 234, 280
MseI TTAA 3 cut(s) 32, 110, 119
MslI CAYNNNNRTG 1 cut(s) 425
MspR9I CCNGG 1 cut(s) 57
Mva1269I GAATGC 3 cut(s) 181, 410, 548
MvaI CCWGG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 392
NdeII GATC 2 cut(s) 52, 308
NlaIII CATG 2 cut(s) 50, 424
NmuCI GTSAC 1 cut(s) 37
PagI TCATGA 1 cut(s) 46
PctI GAATGC 3 cut(s) 181, 410, 548
PfeI GAWTC 2 cut(s) 158, 275
PfoI TCCNGGA 1 cut(s) 55
PkrI GCNGC 4 cut(s) 65, 223, 388, 547
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 55
PspFI CCCAGC 1 cut(s) 395
PspGI CCWGG 1 cut(s) 55
PstI CTGCAG 1 cut(s) 223
RsaI GTAC 2 cut(s) 316, 516
RsaNI GTAC 2 cut(s) 315, 515
RseI CAYNNNNRTG 1 cut(s) 425
SaqAI TTAA 3 cut(s) 32, 110, 119
SatI GCNGC 4 cut(s) 64, 222, 387, 546
Sau3AI GATC 2 cut(s) 52, 308
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 57
SetI ASST 6 cut(s) 13, 32, 187, 388, 397, 495
SfcI CTRYAG 1 cut(s) 219
SmiMI CAYNNNNRTG 1 cut(s) 425
Sse9I AATT 1 cut(s) 129
SsiI CCGC 4 cut(s) 63, 84, 247, 364
SspMI CTAG 1 cut(s) 471
StyD4I CCNGG 1 cut(s) 55
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 2 cut(s) 37, 296
TaqI TCGA 1 cut(s) 382
TasI AATT 1 cut(s) 129
TatI WGTACW 1 cut(s) 314
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 2 cut(s) 158, 275
Tru1I TTAA 3 cut(s) 32, 110, 119
Tru9I TTAA 3 cut(s) 32, 110, 119
TscAI CASTG 3 cut(s) 223, 285, 547
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 3 cut(s) 221, 386, 545
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 4 cut(s) 141, 150, 409, 467
TspRI CASTG 3 cut(s) 223, 285, 547
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.