AT2G45650

Agamous-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009106
Physical Location & Seq
Forward (+)
18804190 .. 18806586
2397 bp
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UTR
Exon/CDS
Intron
AT2G45650.1

Sequence Viewer

Length: 759 bp
ATGGGAAGAGGGAGAGTGGAGATGAAGAGGATAGAGAACAAGATTAATAGACAAGTGACCTTCTCAAAAAGAAGAAACGGTTTGCTGAAGAAAGCTTATGAGCTTTCTGTTCTTTGCGATGCCGAAGTTGCTCTCATCATCTTCTCAAGCCGTGGCAAGCTCTACGAGTTTGGTAGTGTTGGAATTGAAAGCACAATCGAACGGTATAATCGTTGTTACAACTGCTCTCTAAGCAATAATAAGCCTGAAGAGACTACACAGAGTTGGTGTCAGGAGGTGACAAAGCTTAAATCCAAATACGAATCTCTTGTTCGTACTAACAGGAATTTGCTTGGAGAAGATCTTGGAGAAATGGGTGTGAAGGAACTGCAAGCGCTCGAGAGGCAGCTCGAAGCCGCTCTTACCGCGACTCGACAGCGCAAGACACAAGTTATGATGGAAGAAATGGAAGACCTTAGGAAAAAGGAGAGGCAACTAGGAGACATAAACAAACAACTCAAGATTAAGTTTGAAACGGAAGGCCATGCTTTCAAAACCTTTCAAGACTTATGGGCAAACTCGGCGGCATCGGTGGCCGGGGATCCAAACAATTCTGAATTTCCGGTAGAGCCTTCTCATCCTAATGTATTGGATTGCAACACCGAACCCTTTTTACAAATAGGGTTTCAACAACATTACTACGTGCAAGGTGAAGGGTCTTCGGTATCAAAGAGTAACGTGGCAGGTGAGACTAATTTCGTCCAAGGTTGGGTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009553 GO:0009555 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009909 GO:0009911 GO:0010016 GO:0010022 GO:0010073 GO:0010093 GO:0010094 GO:0010154 GO:0010228 GO:0010468 GO:0010556 GO:0010557 GO:0010582 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0035670 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046983 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048439 GO:0048440 GO:0048443 GO:0048444 GO:0048445 GO:0048448 GO:0048449 GO:0048455 GO:0048457 GO:0048459 GO:0048461 GO:0048462 GO:0048466 GO:0048467 GO:0048481 GO:0048507 GO:0048509 GO:0048518 GO:0048522 GO:0048532 GO:0048580 GO:0048582 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0080060 GO:0080090 GO:0080112 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1905393 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

252

Amino Acids

28.74

Weight (kDa)

7.6

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 1.9e-27 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 83 - 170 2e-28 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 713
Acc36I ACCTGC 1 cut(s) 713
AccBSI CCGCTC 1 cut(s) 398
AccII CGCG 1 cut(s) 407
AciI CCGC 3 cut(s) 396, 405, 563
AclWI GGATC 2 cut(s) 575, 588
AcoI YGGCCR 1 cut(s) 573
AcsI RAATTY 2 cut(s) 325, 596
AcuI CTGAAG 2 cut(s) 107, 267
AfaI GTAC 1 cut(s) 316
AfeI AGCGCT 1 cut(s) 375
AfiI CCNNNNNNNGG 1 cut(s) 748
AgsI TTSAA 5 cut(s) 188, 512, 532, 542, 668
AjuI GAANNNNNNNTTGG 1 cut(s) 735
AluBI AGCT 5 cut(s) 95, 103, 160, 286, 388
AluI AGCT 5 cut(s) 95, 103, 160, 286, 388
Alw26I GTCTC 3 cut(s) 245, 474, 722
AlwI GGATC 2 cut(s) 575, 588
Ama87I CYCGRG 1 cut(s) 377
Aor51HI AGCGCT 1 cut(s) 375
AoxI GGCC 2 cut(s) 520, 573
ApeKI GCWGC 1 cut(s) 385
ApoI RAATTY 2 cut(s) 325, 596
AseI ATTAAT 1 cut(s) 45
AspLEI GCGC 2 cut(s) 376, 420
AsuC2I CCSGG 1 cut(s) 577
AsuHPI GGTGA 3 cut(s) 289, 701, 737
AvaI CYCGRG 1 cut(s) 377
AxyI CCTNAGG 1 cut(s) 455
BamHI GGATCC 1 cut(s) 580
BbsI GAAGAC 2 cut(s) 456, 690
BbvI GCAGC 1 cut(s) 397
BccI CCATC 1 cut(s) 430
BceAI ACGGC 1 cut(s) 135
BcnI CCSGG 1 cut(s) 577
BcoDI GTCTC 3 cut(s) 245, 474, 722
BfaI CTAG 1 cut(s) 476
BfoI RGCGCY 1 cut(s) 377
BfuAI ACCTGC 1 cut(s) 713
BglII AGATCT 1 cut(s) 340
BisI GCNGC 3 cut(s) 386, 396, 564
BlsI GCNGC 3 cut(s) 387, 397, 565
Bme1390I CCNGG 1 cut(s) 577
BmeT110I CYCGRG 1 cut(s) 377
BmiI GGNNCC 1 cut(s) 582
BmrFI CCNGG 1 cut(s) 577
BmsI GCATC 2 cut(s) 109, 575
BpiI GAAGAC 2 cut(s) 456, 690
BpuEI CTTGAG 2 cut(s) 130, 482
BpuMI CCSGG 1 cut(s) 577
BsaAI YACGTR 1 cut(s) 682
BsaJI CCNNGG 3 cut(s) 151, 576, 742
BsaWI WCCGGW 1 cut(s) 601
Bsc4I CCNNNNNNNGG 1 cut(s) 748
Bse21I CCTNAGG 1 cut(s) 455
BseDI CCNNGG 3 cut(s) 151, 576, 742
BseGI GGATG 1 cut(s) 616
BseLI CCNNNNNNNGG 1 cut(s) 748
BseXI GCAGC 1 cut(s) 397
Bsh1236I CGCG 1 cut(s) 407
BshFI GGCC 2 cut(s) 522, 575
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 2 cut(s) 576, 602
BslI CCNNNNNNNGG 1 cut(s) 748
BsmAI GTCTC 3 cut(s) 245, 474, 722
BsnI GGCC 2 cut(s) 522, 575
BsoBI CYCGRG 1 cut(s) 377
Bsp143I GATC 2 cut(s) 340, 580
BspACI CCGC 3 cut(s) 396, 405, 563
BspANI GGCC 2 cut(s) 522, 575
BspFNI CGCG 1 cut(s) 407
BspLI GGNNCC 1 cut(s) 582
BspMI ACCTGC 1 cut(s) 713
BspPI GGATC 2 cut(s) 575, 588
BsrBI CCGCTC 1 cut(s) 398
BssECI CCNNGG 3 cut(s) 151, 576, 742
BssMI GATC 2 cut(s) 340, 580
BssT1I CCWWGG 1 cut(s) 742
Bst4CI ACNGT 2 cut(s) 80, 204
Bst6I CTCTTC 2 cut(s) 20, 243
BstBAI YACGTR 1 cut(s) 682
BstC8I GCNNGC 2 cut(s) 158, 372
BstDEI CTNAG 2 cut(s) 230, 455
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 1 cut(s) 616
BstFNI CGCG 1 cut(s) 407
BstH2I RGCGCY 1 cut(s) 377
BstHHI GCGC 2 cut(s) 376, 420
BstKTI GATC 2 cut(s) 343, 583
BstMAI GTCTC 3 cut(s) 245, 474, 722
BstMBI GATC 2 cut(s) 340, 580
BstMWI GCNNNNNNNGC 6 cut(s) 128, 231, 382, 404, 560, 572
BstSCI CCNGG 1 cut(s) 575
BstUI CGCG 1 cut(s) 407
BstV1I GCAGC 1 cut(s) 397
BstV2I GAAGAC 2 cut(s) 456, 690
BstX2I RGATCY 2 cut(s) 340, 580
BstYI RGATCY 2 cut(s) 340, 580
Bsu36I CCTNAGG 1 cut(s) 455
BsuRI GGCC 2 cut(s) 522, 575
BtgI CCRYGG 1 cut(s) 151
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 1 cut(s) 616
BveI ACCTGC 1 cut(s) 713
Cac8I GCNNGC 2 cut(s) 158, 372
CfoI GCGC 2 cut(s) 376, 420
Csp6I GTAC 1 cut(s) 315
CviAII CATG 1 cut(s) 524
CviQI GTAC 1 cut(s) 315
DdeI CTNAG 2 cut(s) 230, 455
DpnI GATC 2 cut(s) 342, 582
DpnII GATC 2 cut(s) 340, 580
EaeI YGGCCR 1 cut(s) 573
Eam1104I CTCTTC 2 cut(s) 20, 243
EarI CTCTTC 2 cut(s) 20, 243
Eco130I CCWWGG 1 cut(s) 742
Eco47III AGCGCT 1 cut(s) 375
Eco57I CTGAAG 2 cut(s) 107, 267
Eco81I CCTNAGG 1 cut(s) 455
Eco88I CYCGRG 1 cut(s) 377
EcoT14I CCWWGG 1 cut(s) 742
ErhI CCWWGG 1 cut(s) 742
FaeI CATG 1 cut(s) 527
FaiI YATR 6 cut(s) 99, 207, 434, 485, 525, 550
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FatI CATG 1 cut(s) 523
Fnu4HI GCNGC 3 cut(s) 386, 396, 564
FokI GGATG 1 cut(s) 603
Fsp4HI GCNGC 3 cut(s) 386, 396, 564
FspBI CTAG 1 cut(s) 476
GlaI GCGC 2 cut(s) 375, 419
GluI GCNGC 3 cut(s) 386, 396, 564
HaeII RGCGCY 1 cut(s) 377
HaeIII GGCC 2 cut(s) 522, 575
HapII CCGG 2 cut(s) 576, 602
HhaI GCGC 2 cut(s) 376, 420
Hin1II CATG 1 cut(s) 527
Hin6I GCGC 2 cut(s) 374, 418
HinP1I GCGC 2 cut(s) 374, 418
HindIII AAGCTT 2 cut(s) 93, 284
HinfI GANTC 2 cut(s) 302, 409
HpaII CCGG 2 cut(s) 576, 602
HphI GGTGA 3 cut(s) 289, 701, 737
Hpy188I TCNGA 1 cut(s) 595
Hpy188III TCNNGA 4 cut(s) 272, 379, 499, 542
HpyAV CCTTC 5 cut(s) 70, 355, 512, 621, 686
HpyCH4III ACNGT 2 cut(s) 80, 204
HpyCH4IV ACGT 2 cut(s) 681, 717
HpyCH4V TGCA 3 cut(s) 370, 636, 685
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 231, 382, 404, 560, 572
HpyF3I CTNAG 2 cut(s) 230, 455
HpySE526I ACGT 2 cut(s) 681, 717
Hsp92II CATG 1 cut(s) 527
HspAI GCGC 2 cut(s) 374, 418
Kzo9I GATC 2 cut(s) 340, 580
LpnPI CCDG 6 cut(s) 257, 258, 307, 589, 615, 708
Lsp1109I GCAGC 1 cut(s) 397
LweI GCATC 2 cut(s) 109, 575
MaeI CTAG 1 cut(s) 476
MaeII ACGT 2 cut(s) 681, 717
MaeIII GTNAC 4 cut(s) 55, 215, 277, 713
MalI GATC 2 cut(s) 342, 582
MbiI CCGCTC 1 cut(s) 398
MboI GATC 2 cut(s) 340, 580
MflI RGATCY 2 cut(s) 340, 580
MluCI AATT 5 cut(s) 183, 325, 589, 596, 733
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 4 cut(s) 21, 268, 375, 462
MseI TTAA 3 cut(s) 45, 288, 504
MslI CAYNNNNRTG 1 cut(s) 621
MspI CCGG 2 cut(s) 576, 602
MspR9I CCNGG 1 cut(s) 577
MvnI CGCG 1 cut(s) 407
MwoI GCNNNNNNNGC 6 cut(s) 128, 231, 382, 404, 560, 572
NciI CCSGG 1 cut(s) 577
NdeII GATC 2 cut(s) 340, 580
NlaIII CATG 1 cut(s) 527
NlaIV GGNNCC 1 cut(s) 582
NmeAIII GCCGAG 1 cut(s) 539
NmuCI GTSAC 2 cut(s) 55, 277
PaeR7I CTCGAG 1 cut(s) 377
PaqCI CACCTGC 1 cut(s) 713
PcsI WCGNNNNNNNCGW 1 cut(s) 208
PfeI GAWTC 1 cut(s) 302
PkrI GCNGC 3 cut(s) 387, 397, 565
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
Ppu21I YACGTR 1 cut(s) 682
PshBI ATTAAT 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 582
PsrI GAACNNNNNNTAC 2 cut(s) 636, 668
PsuI RGATCY 2 cut(s) 340, 580
RsaI GTAC 1 cut(s) 316
RsaNI GTAC 1 cut(s) 315
RseI CAYNNNNRTG 1 cut(s) 621
SaqAI TTAA 3 cut(s) 45, 288, 504
SatI GCNGC 3 cut(s) 386, 396, 564
Sau3AI GATC 2 cut(s) 340, 580
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 1 cut(s) 577
SfaNI GCATC 2 cut(s) 109, 575
Sfr274I CTCGAG 1 cut(s) 377
SlaI CTCGAG 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 621
SmlI CTYRAG 3 cut(s) 145, 377, 497
SmoI CTYRAG 3 cut(s) 145, 377, 497
Sse9I AATT 5 cut(s) 183, 325, 589, 596, 733
SsiI CCGC 3 cut(s) 396, 405, 563
SspMI CTAG 1 cut(s) 476
StyD4I CCNGG 1 cut(s) 575
StyI CCWWGG 1 cut(s) 742
TaaI ACNGT 2 cut(s) 80, 204
TaiI ACGT 2 cut(s) 684, 720
TaqI TCGA 4 cut(s) 198, 378, 390, 412
TasI AATT 5 cut(s) 183, 325, 589, 596, 733
TauI GCSGC 2 cut(s) 398, 566
TfiI GAWTC 1 cut(s) 302
Tru1I TTAA 3 cut(s) 45, 288, 504
Tru9I TTAA 3 cut(s) 45, 288, 504
TseFI GTSAC 2 cut(s) 55, 277
TseI GCWGC 1 cut(s) 385
Tsp45I GTSAC 2 cut(s) 55, 277
TspDTI ATGAA 1 cut(s) 38
TspGWI ACGGA 1 cut(s) 530
VspI ATTAAT 1 cut(s) 45
XapI RAATTY 2 cut(s) 325, 596
XhoI CTCGAG 1 cut(s) 377
XspI CTAG 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.