MD02G1197600.v1.1
MADS Family

MADS-box transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009106
Physical Location & Seq
Reverse (-)
19111227 .. 19116745
5519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1197600.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGGGAGGGGGAGAGTGGAGCTGAAGAGAATAGAGAACAAGATTAACAGGCAGGTGACTTTCTCAAAGAGGAGAAATGGTTTATTGAAGAAAGCCTATGAACTCTCTGTGCTTTGTGATGCTGAGGTTGGCCTTATCATCTTCTCTAGCCGTGGCAAGCTCTATGAGTTTGCCAGCGCTGGTATGAGCAAAACCCTTGAACGATACCAACGTTGCTCCTTCACTCCACCTGAGAATAGCATTGAACGTGAAACACAGAGCTGGTACCAAGAAGTCACAAAACTGAAAGCTAAATATGAATCCCTTCAGCGAACTCAAAGGCATTTGCTTGGTGAAGATCTTGGACCACTGAGTGTGAAGGAGTTGCAAAATCTTGAAAAACAACTTGAAGGAGCACTTGCACAAACCAGACAAAGGAAGACACAGCTTATGATTGAACAAATGGAGGACCTGCGCAAAAAGGAGCGTCACCTTGGAGACCTCAACAAGCAGCTGAGGGTCAAGCTTGAGGCAGAAGGGCAGAATCTGAATGTCATTCAAAACATGTGGAGCTCAGATGCAGCGGCTGGAAGTAGCAACTTTTCTTTGCATTCTTCACAAACCAACCCCATGGACTGTACACCTGAACCTGTCATACAAATGGGGTACCATCCGTATCATCCTGCTGAGGGATCATCTATTCCAAGGAGCTTGACTGGTGAGACCAACTTCATCCAAGGATGGGTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009553 GO:0009555 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009909 GO:0009911 GO:0010016 GO:0010022 GO:0010073 GO:0010093 GO:0010094 GO:0010154 GO:0010228 GO:0010468 GO:0010556 GO:0010557 GO:0010582 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0035670 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046983 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048439 GO:0048440 GO:0048443 GO:0048444 GO:0048445 GO:0048448 GO:0048449 GO:0048455 GO:0048457 GO:0048459 GO:0048461 GO:0048462 GO:0048466 GO:0048467 GO:0048481 GO:0048507 GO:0048509 GO:0048518 GO:0048522 GO:0048532 GO:0048580 GO:0048582 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0080060 GO:0080090 GO:0080112 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1905393 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

244

Amino Acids

27.81

Weight (kDa)

9.18

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 4e-27 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 82 - 169 9.9e-30 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
Acc16I TGCGCA 1 cut(s) 455
Acc36I ACCTGC 2 cut(s) 43, 459
Acc65I GGTACC 2 cut(s) 264, 645
AccB1I GGYRCC 2 cut(s) 264, 645
AciI CCGC 1 cut(s) 563
AclI AACGTT 1 cut(s) 211
AclWI GGATC 1 cut(s) 679
AcuI CTGAAG 2 cut(s) 44, 290
AdeI CACNNNGTG 1 cut(s) 353
AfaI GTAC 3 cut(s) 266, 619, 647
AfeI AGCGCT 1 cut(s) 178
AfiI CCNNNNNNNGG 3 cut(s) 414, 668, 721
AflIII ACRYGT 1 cut(s) 543
AgsI TTSAA 7 cut(s) 88, 200, 245, 377, 389, 437, 539
AjuI GAANNNNNNNTTGG 1 cut(s) 708
AluBI AGCT 9 cut(s) 22, 160, 261, 290, 427, 493, 505, 552, 690
AluI AGCT 9 cut(s) 22, 160, 261, 290, 427, 493, 505, 552, 690
Alw21I GWGCWC 2 cut(s) 397, 554
Alw26I GTCTC 2 cut(s) 471, 695
AlwI GGATC 1 cut(s) 679
AlwNI CAGNNNCTG 2 cut(s) 526, 566
Aor51HI AGCGCT 1 cut(s) 178
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 2 cut(s) 490, 560
Asp700I GAANNNNTTC 1 cut(s) 303
Asp718I GGTACC 2 cut(s) 264, 645
AspLEI GCGC 2 cut(s) 179, 456
AspS9I GGNCC 2 cut(s) 344, 448
AsuHPI GGTGA 4 cut(s) 67, 344, 461, 710
AvaII GGWCC 2 cut(s) 344, 448
BaeI ACNNNNGTAYC 2 cut(s) 196, 229
BanI GGYRCC 2 cut(s) 264, 645
BanII GRGCYC 1 cut(s) 554
BbsI GAAGAC 1 cut(s) 425
Bbv12I GWGCWC 2 cut(s) 397, 554
BbvCI CCTCAGC 3 cut(s) 123, 494, 666
BbvI GCAGC 2 cut(s) 502, 572
BccI CCATC 2 cut(s) 657, 714
BceAI ACGGC 1 cut(s) 135
BcoDI GTCTC 2 cut(s) 471, 695
BfaI CTAG 1 cut(s) 147
BfoI RGCGCY 1 cut(s) 180
BfuAI ACCTGC 2 cut(s) 43, 459
BglII AGATCT 1 cut(s) 337
BisI GCNGC 3 cut(s) 491, 561, 564
BlsI GCNGC 3 cut(s) 492, 562, 565
Bme18I GGWCC 2 cut(s) 344, 448
BmgT120I GGNCC 2 cut(s) 344, 448
BmiI GGNNCC 2 cut(s) 266, 647
BmsI GCATC 2 cut(s) 109, 547
BpiI GAAGAC 1 cut(s) 425
Bpu10I CCTNAGC 3 cut(s) 123, 494, 666
BpuEI CTTGAG 1 cut(s) 527
BsaI GGTCTC 2 cut(s) 471, 695
BsaJI CCNNGG 5 cut(s) 151, 472, 609, 683, 715
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 3 cut(s) 414, 668, 721
Bse1I ACTGG 1 cut(s) 700
BseDI CCNNGG 5 cut(s) 151, 472, 609, 683, 715
BseGI GGATG 4 cut(s) 649, 658, 711, 725
BseLI CCNNNNNNNGG 3 cut(s) 414, 668, 721
BseMII CTCAG 6 cut(s) 114, 222, 341, 485, 567, 657
BseNI ACTGG 1 cut(s) 700
BseRI GAGGAG 1 cut(s) 85
BseXI GCAGC 2 cut(s) 502, 572
BshFI GGCC 1 cut(s) 132
BshNI GGYRCC 2 cut(s) 264, 645
BsiHKAI GWGCWC 2 cut(s) 397, 554
BslI CCNNNNNNNGG 3 cut(s) 414, 668, 721
BsmAI GTCTC 2 cut(s) 471, 695
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 1 cut(s) 132
Bso31I GGTCTC 2 cut(s) 471, 695
Bsp1286I GDGCHC 2 cut(s) 397, 554
Bsp1407I TGTACA 1 cut(s) 617
Bsp143I GATC 2 cut(s) 337, 671
Bsp19I CCATGG 1 cut(s) 609
BspACI CCGC 1 cut(s) 563
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 6 cut(s) 115, 223, 342, 486, 566, 658
BspLI GGNNCC 2 cut(s) 266, 647
BspMI ACCTGC 2 cut(s) 43, 459
BspPI GGATC 1 cut(s) 679
BspT107I GGYRCC 2 cut(s) 264, 645
BspTNI GGTCTC 2 cut(s) 471, 695
BsrGI TGTACA 1 cut(s) 617
BsrI ACTGG 1 cut(s) 700
BssECI CCNNGG 5 cut(s) 151, 472, 609, 683, 715
BssMI GATC 2 cut(s) 337, 671
BssT1I CCWWGG 4 cut(s) 472, 609, 683, 715
Bst4CI ACNGT 1 cut(s) 617
Bst6I CTCTTC 1 cut(s) 20
BstAUI TGTACA 1 cut(s) 617
BstC8I GCNNGC 2 cut(s) 158, 175
BstDEI CTNAG 6 cut(s) 123, 231, 350, 494, 553, 666
BstDSI CCRYGG 2 cut(s) 151, 609
BstF5I GGATG 4 cut(s) 649, 658, 711, 725
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 2 cut(s) 179, 456
BstKTI GATC 2 cut(s) 340, 674
BstMAI GTCTC 2 cut(s) 471, 695
BstMBI GATC 2 cut(s) 337, 671
BstNSI RCATGY 1 cut(s) 547
BstV1I GCAGC 2 cut(s) 502, 572
BstV2I GAAGAC 1 cut(s) 425
BstX2I RGATCY 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 610
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 1 cut(s) 132
BtgI CCRYGG 2 cut(s) 151, 609
BtsCI GGATG 4 cut(s) 649, 658, 711, 725
BtsIMutI CAGTG 1 cut(s) 347
BveI ACCTGC 2 cut(s) 43, 459
Cac8I GCNNGC 2 cut(s) 158, 175
CaiI CAGNNNCTG 2 cut(s) 526, 566
CfoI GCGC 2 cut(s) 179, 456
Cfr13I GGNCC 2 cut(s) 344, 448
CseI GACGC 1 cut(s) 455
Csp6I GTAC 3 cut(s) 265, 618, 646
CspCI CAANNNNNGTGG 2 cut(s) 527, 562
CviAII CATG 2 cut(s) 544, 610
CviQI GTAC 3 cut(s) 265, 618, 646
DdeI CTNAG 6 cut(s) 123, 231, 350, 494, 553, 666
DpnI GATC 2 cut(s) 339, 673
DpnII GATC 2 cut(s) 337, 671
DraIII CACNNNGTG 1 cut(s) 353
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 552
Eco130I CCWWGG 4 cut(s) 472, 609, 683, 715
Eco24I GRGCYC 1 cut(s) 554
Eco31I GGTCTC 2 cut(s) 471, 695
Eco47I GGWCC 2 cut(s) 344, 448
Eco47III AGCGCT 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 552
Eco57I CTGAAG 2 cut(s) 44, 290
EcoICRI GAGCTC 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 448
EcoT14I CCWWGG 4 cut(s) 472, 609, 683, 715
EcoT38I GRGCYC 1 cut(s) 554
ErhI CCWWGG 4 cut(s) 472, 609, 683, 715
FaeI CATG 2 cut(s) 547, 613
FaiI YATR 8 cut(s) 99, 165, 185, 297, 431, 545, 611, 635
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FatI CATG 2 cut(s) 543, 609
Fnu4HI GCNGC 3 cut(s) 491, 561, 564
FokI GGATG 3 cut(s) 636, 645, 698
FriOI GRGCYC 1 cut(s) 554
Fsp4HI GCNGC 3 cut(s) 491, 561, 564
FspBI CTAG 1 cut(s) 147
FspI TGCGCA 1 cut(s) 455
GlaI GCGC 2 cut(s) 178, 455
GluI GCNGC 3 cut(s) 491, 561, 564
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 1 cut(s) 132
HgaI GACGC 1 cut(s) 455
HhaI GCGC 2 cut(s) 179, 456
Hin1II CATG 2 cut(s) 547, 613
Hin6I GCGC 2 cut(s) 177, 454
HinP1I GCGC 2 cut(s) 177, 454
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 2 cut(s) 299, 523
HphI GGTGA 4 cut(s) 67, 344, 461, 710
Hpy166II GTNNAC 1 cut(s) 620
Hpy188I TCNGA 3 cut(s) 528, 556, 731
Hpy188III TCNNGA 1 cut(s) 374
Hpy8I GTNNAC 1 cut(s) 620
HpyAV CCTTC 5 cut(s) 229, 314, 352, 383, 509
HpyCH4III ACNGT 1 cut(s) 617
HpyCH4IV ACGT 2 cut(s) 211, 247
HpyCH4V TGCA 4 cut(s) 367, 401, 560, 589
HpyF3I CTNAG 6 cut(s) 123, 231, 350, 494, 553, 666
HpySE526I ACGT 2 cut(s) 211, 247
Hsp92II CATG 2 cut(s) 547, 613
HspAI GCGC 2 cut(s) 177, 454
KpnI GGTACC 2 cut(s) 268, 649
Kzo9I GATC 2 cut(s) 337, 671
LmnI GCTCC 6 cut(s) 19, 221, 392, 463, 549, 687
Lsp1109I GCAGC 2 cut(s) 502, 572
LweI GCATC 2 cut(s) 109, 547
MaeI CTAG 1 cut(s) 147
MaeII ACGT 2 cut(s) 211, 247
MaeIII GTNAC 3 cut(s) 55, 274, 467
MalI GATC 2 cut(s) 339, 673
MboI GATC 2 cut(s) 337, 671
MboII GAAGA 6 cut(s) 37, 100, 133, 347, 430, 585
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 2 cut(s) 397, 554
MnlI CCTC 7 cut(s) 63, 118, 439, 489, 491, 502, 661
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 1 cut(s) 638
MspA1I CMGCKG 2 cut(s) 493, 563
Mva1269I GAATGC 1 cut(s) 589
NcoI CCATGG 1 cut(s) 609
NdeII GATC 2 cut(s) 337, 671
NlaIII CATG 2 cut(s) 547, 613
NlaIV GGNNCC 2 cut(s) 266, 647
NmuCI GTSAC 3 cut(s) 55, 274, 467
NsbI TGCGCA 1 cut(s) 455
NspI RCATGY 1 cut(s) 547
PaqCI CACCTGC 1 cut(s) 43
PciI ACATGT 1 cut(s) 543
PcsI WCGNNNNNNNCGW 1 cut(s) 208
PctI GAATGC 1 cut(s) 589
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 2 cut(s) 299, 523
PkrI GCNGC 3 cut(s) 492, 562, 565
PpuMI RGGWCCY 1 cut(s) 448
PscI ACATGT 1 cut(s) 543
Psp124BI GAGCTC 1 cut(s) 554
Psp1406I AACGTT 1 cut(s) 211
Psp5II RGGWCCY 1 cut(s) 448
PspN4I GGNNCC 2 cut(s) 266, 647
PspPI GGNCC 2 cut(s) 344, 448
PspPPI RGGWCCY 1 cut(s) 448
PsrI GAACNNNNNNTAC 2 cut(s) 618, 650
PstNI CAGNNNCTG 2 cut(s) 526, 566
PsuI RGATCY 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 3 cut(s) 266, 619, 647
RsaNI GTAC 3 cut(s) 265, 618, 646
RseI CAYNNNNRTG 1 cut(s) 638
SacI GAGCTC 1 cut(s) 554
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 3 cut(s) 491, 561, 564
Sau3AI GATC 2 cut(s) 337, 671
Sau96I GGNCC 2 cut(s) 344, 448
SduI GDGCHC 2 cut(s) 397, 554
SfaNI GCATC 2 cut(s) 109, 547
SinI GGWCC 2 cut(s) 344, 448
SmiMI CAYNNNNRTG 1 cut(s) 638
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
SsiI CCGC 1 cut(s) 563
SspMI CTAG 1 cut(s) 147
SstI GAGCTC 1 cut(s) 554
StyI CCWWGG 4 cut(s) 472, 609, 683, 715
TaaI ACNGT 1 cut(s) 617
TaiI ACGT 2 cut(s) 214, 250
TatI WGTACW 1 cut(s) 617
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 2 cut(s) 299, 523
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 1 cut(s) 354
TseFI GTSAC 3 cut(s) 55, 274, 467
TseI GCWGC 2 cut(s) 490, 560
Tsp45I GTSAC 3 cut(s) 55, 274, 467
TspDTI ATGAA 3 cut(s) 114, 312, 700
TspGWI ACGGA 1 cut(s) 642
TspRI CASTG 1 cut(s) 354
VpaK11BI GGWCC 2 cut(s) 344, 448
XceI RCATGY 1 cut(s) 547
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.