Rw1G020420
MADS Family

MADS-box transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009106
Physical Location & Seq
Forward (+)
44074172 .. 44079035
4864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G020420.1

Sequence Viewer

Length: 738 bp
ATGGGGAGGGGAAGAGTGGAGCTCAAGAGAATAGAGAACAAGATCAACCGCCAGGTGACTTTTTCAAAGCGGAGAAATGGTTTGCTCAAAAAAGCATATGAGCTCTCTGTGCTTTGTGATGCTGAGGTTGCCCTTATCGTCTTCTCTAGTCGAGGCAAGCTCTATGAGTTTTCAAGTGCTGGTATGACCCAAACCCTTGAGAGATACCAACGAAGCAGCTTCAGTCCTCATGACAACGCCAGCAAACGGGAAACAGAGTGCTGGTATGAAGAAGTCTCAAAACTGAGAGCTAAATATGACTCACTTCTGCGTACTCAAAGGCATTTGCTTGGGGAAGACCTTGGACCACTGAGTGTGAAGGAGTTGCAAAATCTTGAAAAACAGCTTGAAGGAGCTCTTACACAAGCCAGACAAAGGAAGACACAGATTATGATTGAACAAATGGAACACCTGCGCAAAAAGGAGCGACAGCTTGGAGACCTGAACCTGCAGCTTAGGGATAAGCTTCAGAAGGAGGGGGAAAATCTCAAAGCCATTCAAGACTTCTGGAGCTCTAATAGTGCAGCAGCTGGGAATAGCAACTTCTTGCATTCTTCACAAGTCAGCCACATTGATCCCGCACCTGAACCCATCTTACAAATTGGGTACCATCACTATGTTGCTGCTGAATCCGCAGATGTTCCGAAGAGCATGGCCATGGCTGCTGAGACCAACTTCATCCAGGGATGGGTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009553 GO:0009555 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009909 GO:0009911 GO:0010016 GO:0010022 GO:0010073 GO:0010093 GO:0010094 GO:0010154 GO:0010228 GO:0010468 GO:0010556 GO:0010557 GO:0010582 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0035670 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046983 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048439 GO:0048440 GO:0048443 GO:0048444 GO:0048445 GO:0048448 GO:0048449 GO:0048455 GO:0048457 GO:0048459 GO:0048461 GO:0048462 GO:0048466 GO:0048467 GO:0048481 GO:0048507 GO:0048509 GO:0048518 GO:0048522 GO:0048532 GO:0048580 GO:0048582 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0080060 GO:0080090 GO:0080112 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1905393 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

245

Amino Acids

28.03

Weight (kDa)

9.11

Isoelectric Point (pI)

51.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 1.2e-27 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 81 - 169 1.1e-27 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 459
Acc16I TGCGCA 1 cut(s) 455
Acc36I ACCTGC 2 cut(s) 459, 495
Acc65I GGTACC 1 cut(s) 645
AccB1I GGYRCC 1 cut(s) 645
AciI CCGC 4 cut(s) 49, 70, 618, 672
AclWI GGATC 1 cut(s) 608
AcoI YGGCCR 1 cut(s) 693
AcuI CTGAAG 2 cut(s) 205, 491
AdeI CACNNNGTG 1 cut(s) 353
AfaI GTAC 2 cut(s) 313, 647
AfiI CCNNNNNNNGG 3 cut(s) 246, 414, 727
AgsI TTSAA 6 cut(s) 66, 174, 377, 389, 437, 539
AjnI CCWGG 2 cut(s) 51, 720
Alw21I GWGCWC 4 cut(s) 24, 105, 397, 554
Alw26I GTCTC 3 cut(s) 280, 471, 701
AlwI GGATC 1 cut(s) 608
AlwNI CAGNNNCTG 1 cut(s) 569
AoxI GGCC 1 cut(s) 693
ApeKI GCWGC 6 cut(s) 216, 490, 563, 566, 662, 701
Asp718I GGTACC 1 cut(s) 645
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 1 cut(s) 67
AvaII GGWCC 1 cut(s) 344
BalI TGGCCA 1 cut(s) 695
BanI GGYRCC 1 cut(s) 645
BanII GRGCYC 4 cut(s) 24, 105, 397, 554
BbsI GAAGAC 3 cut(s) 133, 342, 425
Bbv12I GWGCWC 4 cut(s) 24, 105, 397, 554
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 6 cut(s) 228, 502, 575, 578, 649, 688
BccI CCATC 3 cut(s) 638, 657, 720
BciT130I CCWGG 2 cut(s) 53, 722
BcoDI GTCTC 3 cut(s) 280, 471, 701
BfaI CTAG 1 cut(s) 147
BfmI CTRYAG 1 cut(s) 488
BfuAI ACCTGC 2 cut(s) 459, 495
BisI GCNGC 6 cut(s) 217, 491, 564, 567, 663, 702
BlsI GCNGC 6 cut(s) 218, 492, 565, 568, 664, 703
Bme1390I CCNGG 2 cut(s) 53, 722
Bme18I GGWCC 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 1 cut(s) 647
BmrFI CCNGG 2 cut(s) 53, 722
BmsI GCATC 1 cut(s) 109
BpiI GAAGAC 3 cut(s) 133, 342, 425
BplI GAGNNNNNCTC 3 cut(s) 38, 144, 176
BpmI CTGGAG 1 cut(s) 568
Bpu10I CCTNAGC 2 cut(s) 123, 494
BpuEI CTTGAG 2 cut(s) 8, 218
BsaI GGTCTC 2 cut(s) 471, 701
BsaJI CCNNGG 3 cut(s) 340, 696, 721
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 3 cut(s) 246, 414, 727
BseBI CCWGG 2 cut(s) 53, 722
BseDI CCNNGG 3 cut(s) 340, 696, 721
BseGI GGATG 2 cut(s) 717, 731
BseLI CCNNNNNNNGG 3 cut(s) 246, 414, 727
BseMII CTCAG 4 cut(s) 114, 275, 341, 696
BseXI GCAGC 6 cut(s) 228, 502, 575, 578, 649, 688
BseYI CCCAGC 1 cut(s) 569
BsgI GTGCAG 1 cut(s) 582
BshFI GGCC 1 cut(s) 695
BshNI GGYRCC 1 cut(s) 645
BsiHKAI GWGCWC 4 cut(s) 24, 105, 397, 554
BslI CCNNNNNNNGG 3 cut(s) 246, 414, 727
BsmAI GTCTC 3 cut(s) 280, 471, 701
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 1 cut(s) 695
Bso31I GGTCTC 2 cut(s) 471, 701
Bsp1286I GDGCHC 4 cut(s) 24, 105, 397, 554
Bsp143I GATC 2 cut(s) 42, 613
Bsp19I CCATGG 1 cut(s) 696
BspACI CCGC 4 cut(s) 49, 70, 618, 672
BspANI GGCC 1 cut(s) 695
BspCNI CTCAG 4 cut(s) 115, 276, 342, 697
BspHI TCATGA 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 647
BspMAI CTGCAG 1 cut(s) 492
BspMI ACCTGC 2 cut(s) 459, 495
BspPI GGATC 1 cut(s) 608
BspQI GCTCTTC 1 cut(s) 680
BspT107I GGYRCC 1 cut(s) 645
BspTNI GGTCTC 2 cut(s) 471, 701
BssECI CCNNGG 3 cut(s) 340, 696, 721
BssMI GATC 2 cut(s) 42, 613
BssT1I CCWWGG 2 cut(s) 340, 696
Bst2UI CCWGG 2 cut(s) 53, 722
Bst6I CTCTTC 2 cut(s) 7, 680
BstC8I GCNNGC 2 cut(s) 158, 241
BstDEI CTNAG 5 cut(s) 123, 284, 350, 494, 705
BstDSI CCRYGG 1 cut(s) 696
BstF5I GGATG 2 cut(s) 717, 731
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 2 cut(s) 45, 616
BstMAI GTCTC 3 cut(s) 280, 471, 701
BstMBI GATC 2 cut(s) 42, 613
BstMWI GCNNNNNNNGC 4 cut(s) 109, 128, 671, 701
BstNI CCWGG 2 cut(s) 53, 722
BstSCI CCNGG 2 cut(s) 51, 720
BstSFI CTRYAG 1 cut(s) 488
BstV1I GCAGC 6 cut(s) 228, 502, 575, 578, 649, 688
BstV2I GAAGAC 3 cut(s) 133, 342, 425
BsuRI GGCC 1 cut(s) 695
BtgI CCRYGG 1 cut(s) 696
BtsCI GGATG 2 cut(s) 717, 731
BtsIMutI CAGTG 1 cut(s) 347
BveI ACCTGC 2 cut(s) 459, 495
Cac8I GCNNGC 2 cut(s) 158, 241
CaiI CAGNNNCTG 1 cut(s) 569
CciI TCATGA 1 cut(s) 229
CfoI GCGC 1 cut(s) 456
Cfr13I GGNCC 1 cut(s) 344
Csp6I GTAC 2 cut(s) 312, 646
CviAII CATG 3 cut(s) 230, 691, 697
CviQI GTAC 2 cut(s) 312, 646
DdeI CTNAG 5 cut(s) 123, 284, 350, 494, 705
DpnI GATC 2 cut(s) 44, 615
DpnII GATC 2 cut(s) 42, 613
DraIII CACNNNGTG 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 693
Eam1104I CTCTTC 2 cut(s) 7, 680
EarI CTCTTC 2 cut(s) 7, 680
Ecl136II GAGCTC 4 cut(s) 22, 103, 395, 552
Eco130I CCWWGG 2 cut(s) 340, 696
Eco24I GRGCYC 4 cut(s) 24, 105, 397, 554
Eco31I GGTCTC 2 cut(s) 471, 701
Eco47I GGWCC 1 cut(s) 344
Eco53kI GAGCTC 4 cut(s) 22, 103, 395, 552
Eco57I CTGAAG 2 cut(s) 205, 491
EcoICRI GAGCTC 4 cut(s) 22, 103, 395, 552
EcoRII CCWGG 2 cut(s) 51, 720
EcoT14I CCWWGG 2 cut(s) 340, 696
EcoT38I GRGCYC 4 cut(s) 24, 105, 397, 554
ErhI CCWWGG 2 cut(s) 340, 696
FaeI CATG 3 cut(s) 233, 694, 700
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FatI CATG 3 cut(s) 229, 690, 696
FauI CCCGC 1 cut(s) 625
FauNDI CATATG 1 cut(s) 97
Fnu4HI GCNGC 6 cut(s) 217, 491, 564, 567, 663, 702
FokI GGATG 1 cut(s) 704
FriOI GRGCYC 4 cut(s) 24, 105, 397, 554
Fsp4HI GCNGC 6 cut(s) 217, 491, 564, 567, 663, 702
FspBI CTAG 1 cut(s) 147
FspI TGCGCA 1 cut(s) 455
GlaI GCGC 1 cut(s) 455
GluI GCNGC 6 cut(s) 217, 491, 564, 567, 663, 702
GsaI CCCAGC 1 cut(s) 573
GsuI CTGGAG 1 cut(s) 568
HaeIII GGCC 1 cut(s) 695
HhaI GCGC 1 cut(s) 456
Hin1II CATG 3 cut(s) 233, 694, 700
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 2 cut(s) 299, 668
HphI GGTGA 1 cut(s) 67
Hpy188I TCNGA 2 cut(s) 510, 684
Hpy188III TCNNGA 5 cut(s) 25, 230, 374, 539, 547
HpyAV CCTTC 3 cut(s) 352, 383, 505
HpyCH4V TGCA 4 cut(s) 367, 490, 563, 589
HpyF10VI GCNNNNNNNGC 4 cut(s) 109, 128, 671, 701
HpyF3I CTNAG 5 cut(s) 123, 284, 350, 494, 705
Hsp92II CATG 3 cut(s) 233, 694, 700
HspAI GCGC 1 cut(s) 454
KpnI GGTACC 1 cut(s) 649
Kzo9I GATC 2 cut(s) 42, 613
LguI GCTCTTC 1 cut(s) 680
LmnI GCTCC 4 cut(s) 19, 392, 463, 549
Lsp1109I GCAGC 6 cut(s) 228, 502, 575, 578, 649, 688
LweI GCATC 1 cut(s) 109
MaeI CTAG 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 55
MalI GATC 2 cut(s) 44, 615
MboI GATC 2 cut(s) 42, 613
MboII GAAGA 7 cut(s) 24, 133, 281, 347, 430, 585, 697
MhlI GDGCHC 4 cut(s) 24, 105, 397, 554
MlsI TGGCCA 1 cut(s) 695
MluCI AATT 1 cut(s) 639
MluNI TGGCCA 1 cut(s) 695
MlyI GAGTC 1 cut(s) 293
MnlI CCTC 4 cut(s) 118, 146, 237, 508
Mox20I TGGCCA 1 cut(s) 695
MscI TGGCCA 1 cut(s) 695
MslI CAYNNNNRTG 2 cut(s) 654, 695
Msp20I TGGCCA 1 cut(s) 695
MspA1I CMGCKG 1 cut(s) 569
MspR9I CCNGG 2 cut(s) 53, 722
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 2 cut(s) 53, 722
MwoI GCNNNNNNNGC 4 cut(s) 109, 128, 671, 701
NcoI CCATGG 1 cut(s) 696
NdeI CATATG 1 cut(s) 97
NdeII GATC 2 cut(s) 42, 613
NlaIII CATG 3 cut(s) 233, 694, 700
NlaIV GGNNCC 1 cut(s) 647
NmuCI GTSAC 1 cut(s) 55
NsbI TGCGCA 1 cut(s) 455
PagI TCATGA 1 cut(s) 229
PaqCI CACCTGC 1 cut(s) 459
PciSI GCTCTTC 1 cut(s) 680
PctI GAATGC 1 cut(s) 589
PfeI GAWTC 1 cut(s) 668
PkrI GCNGC 6 cut(s) 218, 492, 565, 568, 664, 703
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
Psp124BI GAGCTC 4 cut(s) 24, 105, 397, 554
Psp6I CCWGG 2 cut(s) 51, 720
PspFI CCCAGC 1 cut(s) 569
PspGI CCWGG 2 cut(s) 51, 720
PspN4I GGNNCC 1 cut(s) 647
PspPI GGNCC 1 cut(s) 344
PsrI GAACNNNNNNTAC 2 cut(s) 618, 650
PstI CTGCAG 1 cut(s) 492
PstNI CAGNNNCTG 1 cut(s) 569
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 2 cut(s) 313, 647
RsaNI GTAC 2 cut(s) 312, 646
RseI CAYNNNNRTG 2 cut(s) 654, 695
SacI GAGCTC 4 cut(s) 24, 105, 397, 554
SapI GCTCTTC 1 cut(s) 680
SatI GCNGC 6 cut(s) 217, 491, 564, 567, 663, 702
Sau3AI GATC 2 cut(s) 42, 613
Sau96I GGNCC 1 cut(s) 344
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 2 cut(s) 53, 722
SduI GDGCHC 4 cut(s) 24, 105, 397, 554
SfaNI GCATC 1 cut(s) 109
SfcI CTRYAG 1 cut(s) 488
SinI GGWCC 1 cut(s) 344
SmiMI CAYNNNNRTG 2 cut(s) 654, 695
SmlI CTYRAG 2 cut(s) 23, 197
SmoI CTYRAG 2 cut(s) 23, 197
Sse9I AATT 1 cut(s) 639
SsiI CCGC 4 cut(s) 49, 70, 618, 672
SspMI CTAG 1 cut(s) 147
SstI GAGCTC 4 cut(s) 24, 105, 397, 554
StyD4I CCNGG 2 cut(s) 51, 720
StyI CCWWGG 2 cut(s) 340, 696
TaqI TCGA 1 cut(s) 151
TasI AATT 1 cut(s) 639
TfiI GAWTC 1 cut(s) 668
TscAI CASTG 1 cut(s) 354
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 6 cut(s) 216, 490, 563, 566, 662, 701
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 2 cut(s) 282, 706
TspRI CASTG 1 cut(s) 354
VpaK11BI GGWCC 1 cut(s) 344
XspI CTAG 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.