AT2G45850

DNA-binding family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011861
Physical Location & Seq
Reverse (-)
18871479 .. 18873972
2494 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45850.3

Sequence Viewer

Length: 1047 bp
ATGGATCGAAGAGATGCAATGGGATTATCCGGGTCAGGTTCTTACTATATCCATAGAGGATTATCCGGGTCGGGTCCTCCAACGTTTCATGGATCACCACAGCAACAGCAAGGTCTTCGTCACTTACCTAATCAAAACTCTCCATTCGGGTCAGGCTCCACTGGTTTCGGATCTCCTTCTTTACACGGTGATCCTTCTCTGGCAACAGCAGCCGGAGGAGCCGGAGCTCTTCCTCATCATATCGGCGTTAATATGATTGCTCCTCCTCCACCTCCCAGTGAAACTCCGATGAAACGAAAGAGAGGACGGCCTAGAAAATACGGTCAAGACGGCTCTGTTTCTTTGGCTCTGTCGTCTTCCTCTGTTTCGACCATTACTCCCAACAACTCTAACAAACGCGGCCGTGGTCGACCTCCGGGCTCCGGCAAGAAACAGAGAATGGCTTCCGTTGGTGAACTGATGCCTTCATCTTCTGGAATGAGCTTCACGCCACATGTTATCGCGGTTTCAATAGGAGAAGATATTGCATCAAAGGTTATAGCTTTCTCTCAACAAGGTCCGAGAGCCATTTGCGTTTTATCTGCAAGTGGTGCAGTCTCTACTGCAACACTTATTCAACCATCAGCATCTCCCGGAGCCATTAAATACGAGGGCCGGTTTGAAATCCTAGCGTTATCAACATCTTATATAGTGGCAACTGATGGAAGCTTCCGTAACCGAACTGGAAACTTATCGGTTTCGCTTGCTAGCCCCGATGGGCGTGTGATTGGCGGTGCCATTGGTGGGCCTTTAATAGCTGCAAGTCCTGTTCAGGTTATTGTAGGGAGCTTTATATGGGCAGCTCCAAAGATCAAGAGCAAGAAACGAGAAGAAGAAGCTTCTGAAGTTGTTCAAGAAACTGATGATCACCACGTTCTGGACAATAATAACAACACGATTTCGCCTGTCCCTCAGCAGCAGCCAAACCAAAACCTGATTTGGTCAACAGGTTCAAGGCAAATGGATATGCGTCATGCTCATGCTGATATTGATTTAATGCGCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

348

Amino Acids

36.33

Weight (kDa)

9.94

Isoelectric Point (pI)

50.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 163 - 275 1.3e-20 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 773
AccB7I CCANNNNNTGG 1 cut(s) 916
AccI GTMKAC 1 cut(s) 409
AccII CGCG 3 cut(s) 399, 503, 1041
AciI CCGC 4 cut(s) 399, 503, 771, 1041
AclI AACGTT 1 cut(s) 83
AclWI GGATC 4 cut(s) 12, 100, 178, 185
AcoI YGGCCR 1 cut(s) 400
AcuI CTGAAG 1 cut(s) 903
AfiI CCNNNNNNNGG 3 cut(s) 422, 783, 916
AflIII ACRYGT 1 cut(s) 493
AgsI TTSAA 5 cut(s) 510, 617, 662, 893, 993
AluBI AGCT 8 cut(s) 227, 483, 542, 708, 797, 828, 842, 878
AluI AGCT 8 cut(s) 227, 483, 542, 708, 797, 828, 842, 878
Alw21I GWGCWC 1 cut(s) 229
Alw26I GTCTC 1 cut(s) 601
AlwI GGATC 4 cut(s) 12, 100, 178, 185
AoxI GGCC 4 cut(s) 308, 400, 652, 785
ApeKI GCWGC 5 cut(s) 209, 797, 839, 955, 958
Asp700I GAANNNNTTC 2 cut(s) 442, 888
AspLEI GCGC 1 cut(s) 1041
AspS9I GGNCC 4 cut(s) 74, 557, 652, 785
AsuC2I CCSGG 4 cut(s) 31, 67, 417, 633
AsuHPI GGTGA 4 cut(s) 87, 200, 464, 899
AsuNHI GCTAGC 1 cut(s) 746
AvaII GGWCC 2 cut(s) 74, 557
BanI GGYRCC 1 cut(s) 773
BanII GRGCYC 2 cut(s) 229, 422
BbsI GAAGAC 2 cut(s) 107, 348
Bbv12I GWGCWC 1 cut(s) 229
BbvCI CCTCAGC 1 cut(s) 951
BbvI GCAGC 5 cut(s) 221, 784, 851, 967, 970
BccI CCATC 3 cut(s) 628, 695, 749
BceAI ACGGC 3 cut(s) 323, 346, 387
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BclI TGATCA 1 cut(s) 904
BcnI CCSGG 4 cut(s) 31, 67, 417, 633
BcoDI GTCTC 1 cut(s) 601
BfaI CTAG 3 cut(s) 312, 668, 747
BisI GCNGC 6 cut(s) 210, 400, 798, 840, 956, 959
BlsI GCNGC 6 cut(s) 211, 401, 799, 841, 957, 960
Bme1390I CCNGG 4 cut(s) 31, 67, 417, 633
Bme18I GGWCC 2 cut(s) 74, 557
BmgT120I GGNCC 4 cut(s) 74, 557, 652, 785
BmiI GGNNCC 6 cut(s) 75, 157, 220, 421, 637, 775
BmrFI CCNGG 4 cut(s) 31, 67, 417, 633
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 4 cut(s) 4, 450, 536, 635
BmtI GCTAGC 1 cut(s) 750
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 2 cut(s) 107, 348
Bpu10I CCTNAGC 1 cut(s) 951
BpuMI CCSGG 4 cut(s) 31, 67, 417, 633
BsaJI CCNNGG 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 361, 391
Bsc4I CCNNNNNNNGG 3 cut(s) 422, 783, 916
Bse118I RCCGGY 1 cut(s) 654
Bse1I ACTGG 3 cut(s) 166, 276, 727
Bse3DI GCAATG 1 cut(s) 24
BseDI CCNNGG 1 cut(s) 403
BseLI CCNNNNNNNGG 3 cut(s) 422, 783, 916
BseMI GCAATG 1 cut(s) 24
BseMII CTCAG 1 cut(s) 965
BseNI ACTGG 3 cut(s) 166, 276, 727
BseRI GAGGAG 3 cut(s) 231, 252, 255
BseX3I CGGCCG 1 cut(s) 400
BseXI GCAGC 5 cut(s) 221, 784, 851, 967, 970
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 3 cut(s) 399, 503, 1041
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 4 cut(s) 310, 402, 654, 787
BshNI GGYRCC 1 cut(s) 773
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 1 cut(s) 229
BsiSI CCGG 8 cut(s) 30, 66, 213, 222, 416, 423, 633, 655
BslFI GGGAC 1 cut(s) 932
BslI CCNNNNNNNGG 3 cut(s) 422, 783, 916
BsmAI GTCTC 1 cut(s) 601
BsmFI GGGAC 1 cut(s) 932
BsnI GGCC 4 cut(s) 310, 402, 654, 787
Bsp1286I GDGCHC 2 cut(s) 229, 422
Bsp143I GATC 6 cut(s) 4, 92, 170, 190, 849, 904
BspACI CCGC 4 cut(s) 399, 503, 771, 1041
BspANI GGCC 4 cut(s) 310, 402, 654, 787
BspCNI CTCAG 1 cut(s) 964
BspFNI CGCG 3 cut(s) 399, 503, 1041
BspLI GGNNCC 6 cut(s) 75, 157, 220, 421, 637, 775
BspOI GCTAGC 1 cut(s) 750
BspPI GGATC 4 cut(s) 12, 100, 178, 185
BspQI GCTCTTC 1 cut(s) 234
BspT107I GGYRCC 1 cut(s) 773
BsrDI GCAATG 1 cut(s) 24
BsrFI RCCGGY 1 cut(s) 654
BsrI ACTGG 3 cut(s) 166, 276, 727
BssAI RCCGGY 1 cut(s) 654
BssECI CCNNGG 1 cut(s) 403
BssMI GATC 6 cut(s) 4, 92, 170, 190, 849, 904
Bst4CI ACNGT 2 cut(s) 188, 323
Bst6I CTCTTC 2 cut(s) 4, 234
BstAPI GCANNNNNTGC 1 cut(s) 590
BstC8I GCNNGC 2 cut(s) 744, 748
BstDEI CTNAG 1 cut(s) 951
BstDSI CCRYGG 1 cut(s) 403
BstFNI CGCG 3 cut(s) 399, 503, 1041
BstHHI GCGC 1 cut(s) 1041
BstKTI GATC 6 cut(s) 7, 95, 173, 193, 852, 907
BstMAI GTCTC 1 cut(s) 601
BstMBI GATC 6 cut(s) 4, 92, 170, 190, 849, 904
BstMCI CGRYCG 1 cut(s) 403
BstMWI GCNNNNNNNGC 3 cut(s) 209, 218, 590
BstNSI RCATGY 1 cut(s) 497
BstSCI CCNGG 4 cut(s) 29, 65, 415, 631
BstUI CGCG 3 cut(s) 399, 503, 1041
BstV1I GCAGC 5 cut(s) 221, 784, 851, 967, 970
BstV2I GAAGAC 2 cut(s) 107, 348
BstX2I RGATCY 1 cut(s) 170
BstYI RGATCY 1 cut(s) 170
BstZI CGGCCG 1 cut(s) 400
BsuRI GGCC 4 cut(s) 310, 402, 654, 787
BtgI CCRYGG 1 cut(s) 403
BtsIMutI CAGTG 2 cut(s) 159, 283
Cac8I GCNNGC 2 cut(s) 744, 748
CfoI GCGC 1 cut(s) 1041
Cfr10I RCCGGY 1 cut(s) 654
Cfr13I GGNCC 4 cut(s) 74, 557, 652, 785
CseI GACGC 1 cut(s) 998
CviAII CATG 4 cut(s) 89, 494, 1013, 1019
DdeI CTNAG 1 cut(s) 951
DpnI GATC 6 cut(s) 6, 94, 172, 192, 851, 906
DpnII GATC 6 cut(s) 4, 92, 170, 190, 849, 904
EaeI YGGCCR 1 cut(s) 400
EagI CGGCCG 1 cut(s) 400
Eam1104I CTCTTC 2 cut(s) 4, 234
EarI CTCTTC 2 cut(s) 4, 234
Ecl136II GAGCTC 1 cut(s) 227
EclXI CGGCCG 1 cut(s) 400
Eco24I GRGCYC 2 cut(s) 229, 422
Eco47I GGWCC 2 cut(s) 74, 557
Eco52I CGGCCG 1 cut(s) 400
Eco53kI GAGCTC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 903
EcoICRI GAGCTC 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 74
EcoT38I GRGCYC 2 cut(s) 229, 422
FaeI CATG 4 cut(s) 92, 497, 1016, 1022
FaqI GGGAC 1 cut(s) 932
FatI CATG 4 cut(s) 88, 493, 1012, 1018
FbaI TGATCA 1 cut(s) 904
FblI GTMKAC 1 cut(s) 409
Fnu4HI GCNGC 6 cut(s) 210, 400, 798, 840, 956, 959
FriOI GRGCYC 2 cut(s) 229, 422
Fsp4HI GCNGC 6 cut(s) 210, 400, 798, 840, 956, 959
FspBI CTAG 3 cut(s) 312, 668, 747
GlaI GCGC 1 cut(s) 1040
GluI GCNGC 6 cut(s) 210, 400, 798, 840, 956, 959
HaeIII GGCC 4 cut(s) 310, 402, 654, 787
HapII CCGG 8 cut(s) 30, 66, 213, 222, 416, 423, 633, 655
HgaI GACGC 1 cut(s) 998
HhaI GCGC 1 cut(s) 1041
Hin1II CATG 4 cut(s) 92, 497, 1016, 1022
Hin6I GCGC 1 cut(s) 1039
HinP1I GCGC 1 cut(s) 1039
HincII GTYRAC 2 cut(s) 410, 984
HindII GTYRAC 2 cut(s) 410, 984
HindIII AAGCTT 2 cut(s) 706, 876
HpaII CCGG 8 cut(s) 30, 66, 213, 222, 416, 423, 633, 655
HphI GGTGA 4 cut(s) 87, 200, 464, 899
Hpy166II GTNNAC 3 cut(s) 410, 455, 984
Hpy188I TCNGA 4 cut(s) 170, 288, 561, 883
Hpy188III TCNNGA 5 cut(s) 326, 474, 853, 893, 917
Hpy8I GTNNAC 3 cut(s) 410, 455, 984
HpyAV CCTTC 3 cut(s) 186, 204, 474
HpyCH4III ACNGT 2 cut(s) 188, 323
HpyCH4IV ACGT 2 cut(s) 83, 912
HpyCH4V TGCA 6 cut(s) 17, 527, 584, 593, 605, 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 218, 590
HpyF3I CTNAG 1 cut(s) 951
HpySE526I ACGT 2 cut(s) 83, 912
Hsp92II CATG 4 cut(s) 92, 497, 1016, 1022
HspAI GCGC 1 cut(s) 1039
Ksp22I TGATCA 1 cut(s) 904
Kzo9I GATC 6 cut(s) 4, 92, 170, 190, 849, 904
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 8 cut(s) 161, 218, 224, 265, 425, 635, 825, 847
Lsp1109I GCAGC 5 cut(s) 221, 784, 851, 967, 970
LweI GCATC 4 cut(s) 4, 450, 536, 635
MaeI CTAG 3 cut(s) 312, 668, 747
MaeII ACGT 2 cut(s) 83, 912
MaeIII GTNAC 2 cut(s) 119, 713
MalI GATC 6 cut(s) 6, 94, 172, 192, 851, 906
MboI GATC 6 cut(s) 4, 92, 170, 190, 849, 904
MboII GAAGA 8 cut(s) 21, 107, 221, 348, 462, 530, 881, 884
MflI RGATCY 1 cut(s) 170
MhlI GDGCHC 2 cut(s) 229, 422
MmeI TCCRAC 1 cut(s) 104
MroXI GAANNNNTTC 2 cut(s) 442, 888
MseI TTAA 4 cut(s) 249, 642, 791, 1034
MslI CAYNNNNRTG 1 cut(s) 1017
MspI CCGG 8 cut(s) 30, 66, 213, 222, 416, 423, 633, 655
MspR9I CCNGG 4 cut(s) 31, 67, 417, 633
MvnI CGCG 3 cut(s) 399, 503, 1041
MwoI GCNNNNNNNGC 3 cut(s) 209, 218, 590
NciI CCSGG 4 cut(s) 31, 67, 417, 633
NdeII GATC 6 cut(s) 4, 92, 170, 190, 849, 904
NheI GCTAGC 1 cut(s) 746
NlaIII CATG 4 cut(s) 92, 497, 1016, 1022
NlaIV GGNNCC 6 cut(s) 75, 157, 220, 421, 637, 775
NmuCI GTSAC 1 cut(s) 119
NspI RCATGY 1 cut(s) 497
PciI ACATGT 1 cut(s) 493
PciSI GCTCTTC 1 cut(s) 234
PdmI GAANNNNTTC 2 cut(s) 442, 888
PflMI CCANNNNNTGG 1 cut(s) 916
PfoI TCCNGGA 1 cut(s) 631
PkrI GCNGC 6 cut(s) 211, 401, 799, 841, 957, 960
PpuMI RGGWCCY 1 cut(s) 74
PscI ACATGT 1 cut(s) 493
Psp124BI GAGCTC 1 cut(s) 229
Psp1406I AACGTT 1 cut(s) 83
Psp5II RGGWCCY 1 cut(s) 74
PspN4I GGNNCC 6 cut(s) 75, 157, 220, 421, 637, 775
PspPI GGNCC 4 cut(s) 74, 557, 652, 785
PspPPI RGGWCCY 1 cut(s) 74
PsuI RGATCY 1 cut(s) 170
RseI CAYNNNNRTG 1 cut(s) 1017
SacI GAGCTC 1 cut(s) 229
SalI GTCGAC 1 cut(s) 408
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 4 cut(s) 249, 642, 791, 1034
SatI GCNGC 6 cut(s) 210, 400, 798, 840, 956, 959
Sau3AI GATC 6 cut(s) 4, 92, 170, 190, 849, 904
Sau96I GGNCC 4 cut(s) 74, 557, 652, 785
ScrFI CCNGG 4 cut(s) 31, 67, 417, 633
SduI GDGCHC 2 cut(s) 229, 422
SfaNI GCATC 4 cut(s) 4, 450, 536, 635
SinI GGWCC 2 cut(s) 74, 557
SmiMI CAYNNNNRTG 1 cut(s) 1017
SsiI CCGC 4 cut(s) 399, 503, 771, 1041
SspMI CTAG 3 cut(s) 312, 668, 747
SstI GAGCTC 1 cut(s) 229
StyD4I CCNGG 4 cut(s) 29, 65, 415, 631
TaaI ACNGT 2 cut(s) 188, 323
TaiI ACGT 2 cut(s) 86, 915
TaqI TCGA 3 cut(s) 7, 368, 409
TauI GCSGC 1 cut(s) 402
Tru1I TTAA 4 cut(s) 249, 642, 791, 1034
Tru9I TTAA 4 cut(s) 249, 642, 791, 1034
TscAI CASTG 2 cut(s) 166, 283
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 5 cut(s) 209, 797, 839, 955, 958
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 3 cut(s) 77, 305, 456
TspGWI ACGGA 2 cut(s) 436, 701
TspRI CASTG 2 cut(s) 166, 283
Van91I CCANNNNNTGG 1 cut(s) 916
VpaK11BI GGWCC 2 cut(s) 74, 557
XceI RCATGY 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 409
XmnI GAANNNNTTC 2 cut(s) 442, 888
XspI CTAG 3 cut(s) 312, 668, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.