RLG00000034994

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011861
Physical Location & Seq
Reverse (-)
58777887 .. 58780171
2285 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034994

Sequence Viewer

Length: 666 bp
ATGGTAATCTCAAATCCGGAGCCAAACAGCAGTAAACAGCTGGTGGAGTGTCCTCCACAGGCTGACCCTATCGTCAATGAGTTGGTGGAATCTCCTCCACAATCTGACCCCATTGTTGATGAGTTGTTGGAGTCTCCTCCACAGTCTGAGTTTGACCCCATCGCTGATGAGAACAATGCAACATTGTCAGCCGAAAAAGGCGGTCAATCGGAGGCAGGTGAAGTGATGATGACACCCAAGAAGAAGAGGGGGAGACATAGGAAGTACAAGGTCAATGAAGAAACGGTCTCTGCCGTTCCGGCTTCCAACTCGGAGGGCTCCTCCAAGAAAGCAAAAGGCCGCCACAAAGGCTCAGGCAATAAATTGCAGTCGCTCGTTGTCAATGAAACTGGAAGAGCTGGAAACTTCATCCCTCTCGCAGTGACTGTGCAAACTGGGGAGGACCTTCTCCATAAAATATGGGTCATGGGACAGAGGGACATCAAACAATTTGCATTCTTTCAGCTACTGGGTTGCTATGATATCATCACTCTCTTTGGATCGTTTGTATACCATGCAATGTATCCACACAGAAAATTGGGCATGATAAGTATTCTACTGGCTAATCCCGATAATACAGTTTTTGACTTTTTGGTGGCTCTGTTTTATCAGGAGCACCGCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

222

Amino Acids

24.44

Weight (kDa)

5.89

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 206
AasI GACNNNNNNGTC 1 cut(s) 71
Acc36I ACCTGC 1 cut(s) 206
AccBSI CCGCTC 1 cut(s) 660
AccI GTMKAC 1 cut(s) 549
AccIII TCCGGA 1 cut(s) 16
AciI CCGC 3 cut(s) 201, 340, 658
AclWI GGATC 1 cut(s) 547
AfaI GTAC 1 cut(s) 266
AluBI AGCT 3 cut(s) 40, 398, 505
AluI AGCT 3 cut(s) 40, 398, 505
Alw21I GWGCWC 1 cut(s) 657
Alw26I GTCTC 3 cut(s) 138, 247, 292
AlwI GGATC 1 cut(s) 547
AlwNI CAGNNNCTG 2 cut(s) 425, 508
Aor13HI TCCGGA 1 cut(s) 16
AoxI GGCC 1 cut(s) 337
AspS9I GGNCC 1 cut(s) 442
AsuHPI GGTGA 1 cut(s) 230
AvaII GGWCC 1 cut(s) 442
BanII GRGCYC 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 657
BccI CCATC 1 cut(s) 167
BceAI ACGGC 1 cut(s) 278
BciVI GTATCC 1 cut(s) 573
BcoDI GTCTC 3 cut(s) 138, 247, 292
BfuAI ACCTGC 1 cut(s) 206
BfuI GTATCC 1 cut(s) 573
BglI GCCNNNNNGGC 2 cut(s) 299, 348
BisI GCNGC 1 cut(s) 340
BlsI GCNGC 1 cut(s) 341
Bme18I GGWCC 1 cut(s) 442
BmgT120I GGNCC 1 cut(s) 442
BmiI GGNNCC 2 cut(s) 21, 319
BmrI ACTGGG 2 cut(s) 444, 518
BmuI ACTGGG 2 cut(s) 444, 518
BplI GAGNNNNNCTC 3 cut(s) 305, 337, 644
Bpu10I CCTNAGC 1 cut(s) 352
BsaI GGTCTC 1 cut(s) 292
BsaWI WCCGGW 1 cut(s) 16
Bse1I ACTGG 4 cut(s) 394, 439, 513, 603
Bse3DI GCAATG 1 cut(s) 564
BseAI TCCGGA 1 cut(s) 16
BseGI GGATG 1 cut(s) 408
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 2 cut(s) 138, 366
BseNI ACTGG 4 cut(s) 394, 439, 513, 603
BseRI GAGGAG 3 cut(s) 84, 126, 310
BshFI GGCC 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 657
BsiSI CCGG 2 cut(s) 17, 299
BslFI GGGAC 2 cut(s) 483, 491
BsmAI GTCTC 3 cut(s) 138, 247, 292
BsmFI GGGAC 2 cut(s) 483, 491
BsmI GAATGC 1 cut(s) 494
BsnI GGCC 1 cut(s) 339
Bso31I GGTCTC 1 cut(s) 292
Bsp1286I GDGCHC 2 cut(s) 320, 657
Bsp13I TCCGGA 1 cut(s) 16
Bsp143I GATC 1 cut(s) 539
BspACI CCGC 3 cut(s) 201, 340, 658
BspANI GGCC 1 cut(s) 339
BspCNI CTCAG 2 cut(s) 139, 365
BspEI TCCGGA 1 cut(s) 16
BspLI GGNNCC 2 cut(s) 21, 319
BspMI ACCTGC 1 cut(s) 206
BspPI GGATC 1 cut(s) 547
BspQI GCTCTTC 1 cut(s) 388
BspTNI GGTCTC 1 cut(s) 292
BsrBI CCGCTC 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 564
BsrI ACTGG 4 cut(s) 394, 439, 513, 603
BssMI GATC 1 cut(s) 539
BssNAI GTATAC 1 cut(s) 550
Bst1107I GTATAC 1 cut(s) 550
Bst4CI ACNGT 4 cut(s) 144, 286, 427, 619
Bst6I CTCTTC 2 cut(s) 239, 388
BstDEI CTNAG 2 cut(s) 147, 352
BstF5I GGATG 1 cut(s) 408
BstKTI GATC 1 cut(s) 542
BstMAI GTCTC 3 cut(s) 138, 247, 292
BstMBI GATC 1 cut(s) 539
BstMWI GCNNNNNNNGC 2 cut(s) 299, 348
BstZ17I GTATAC 1 cut(s) 550
BsuI GTATCC 1 cut(s) 573
BsuRI GGCC 1 cut(s) 339
BtgZI GCGATG 1 cut(s) 145
BtsCI GGATG 1 cut(s) 408
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 426
BveI ACCTGC 1 cut(s) 206
CaiI CAGNNNCTG 2 cut(s) 425, 508
Cfr13I GGNCC 1 cut(s) 442
Csp6I GTAC 1 cut(s) 265
CviAII CATG 3 cut(s) 466, 554, 583
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 2 cut(s) 147, 352
DpnI GATC 1 cut(s) 541
DpnII GATC 1 cut(s) 539
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
Eam1104I CTCTTC 2 cut(s) 239, 388
EarI CTCTTC 2 cut(s) 239, 388
Eco24I GRGCYC 1 cut(s) 320
Eco31I GGTCTC 1 cut(s) 292
Eco32I GATATC 1 cut(s) 523
Eco47I GGWCC 1 cut(s) 442
EcoO109I RGGNCCY 1 cut(s) 442
EcoRV GATATC 1 cut(s) 523
EcoT38I GRGCYC 1 cut(s) 320
FaeI CATG 3 cut(s) 469, 557, 586
FaiI YATR 9 cut(s) 258, 453, 460, 467, 519, 550, 555, 584, 664
FaqI GGGAC 2 cut(s) 483, 491
FatI CATG 3 cut(s) 465, 553, 582
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 1 cut(s) 340
FokI GGATG 1 cut(s) 395
FriOI GRGCYC 1 cut(s) 320
Fsp4HI GCNGC 1 cut(s) 340
GluI GCNGC 1 cut(s) 340
HaeIII GGCC 1 cut(s) 339
HapII CCGG 2 cut(s) 17, 299
Hin1II CATG 3 cut(s) 469, 557, 586
HinfI GANTC 2 cut(s) 89, 131
HpaII CCGG 2 cut(s) 17, 299
HphI GGTGA 1 cut(s) 230
Hpy166II GTNNAC 2 cut(s) 35, 550
Hpy188I TCNGA 4 cut(s) 106, 148, 211, 313
Hpy188III TCNNGA 3 cut(s) 17, 608, 650
Hpy8I GTNNAC 2 cut(s) 35, 550
HpyAV CCTTC 1 cut(s) 455
HpyCH4III ACNGT 4 cut(s) 144, 286, 427, 619
HpyCH4V TGCA 5 cut(s) 179, 367, 430, 494, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 299, 348
HpyF3I CTNAG 2 cut(s) 147, 352
Hsp92II CATG 3 cut(s) 469, 557, 586
Kpn2I TCCGGA 1 cut(s) 16
Kzo9I GATC 1 cut(s) 539
LguI GCTCTTC 1 cut(s) 388
LmnI GCTCC 3 cut(s) 19, 323, 652
MaeIII GTNAC 1 cut(s) 421
MalI GATC 1 cut(s) 541
MbiI CCGCTC 1 cut(s) 660
MboI GATC 1 cut(s) 539
MboII GAAGA 4 cut(s) 253, 256, 290, 405
MhlI GDGCHC 2 cut(s) 320, 657
MluCI AATT 3 cut(s) 362, 488, 575
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 2 cut(s) 108, 330
MroI TCCGGA 1 cut(s) 16
MspA1I CMGCKG 1 cut(s) 40
MspI CCGG 2 cut(s) 17, 299
Mva1269I GAATGC 1 cut(s) 494
MwoI GCNNNNNNNGC 2 cut(s) 299, 348
NdeII GATC 1 cut(s) 539
NlaIII CATG 3 cut(s) 469, 557, 586
NlaIV GGNNCC 2 cut(s) 21, 319
NmuCI GTSAC 1 cut(s) 421
PaqCI CACCTGC 1 cut(s) 206
PciSI GCTCTTC 1 cut(s) 388
PctI GAATGC 1 cut(s) 494
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 1 cut(s) 341
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PpuMI RGGWCCY 1 cut(s) 442
Psp5II RGGWCCY 1 cut(s) 442
PspN4I GGNNCC 2 cut(s) 21, 319
PspPI GGNCC 1 cut(s) 442
PspPPI RGGWCCY 1 cut(s) 442
PstNI CAGNNNCTG 2 cut(s) 425, 508
PvuII CAGCTG 1 cut(s) 40
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
SapI GCTCTTC 1 cut(s) 388
SatI GCNGC 1 cut(s) 340
Sau3AI GATC 1 cut(s) 539
Sau96I GGNCC 1 cut(s) 442
SchI GAGTC 1 cut(s) 140
SduI GDGCHC 2 cut(s) 320, 657
SetI ASST 6 cut(s) 42, 220, 273, 400, 447, 507
SinI GGWCC 1 cut(s) 442
Sse9I AATT 3 cut(s) 362, 488, 575
SsiI CCGC 3 cut(s) 201, 340, 658
TaaI ACNGT 4 cut(s) 144, 286, 427, 619
TasI AATT 3 cut(s) 362, 488, 575
TatI WGTACW 1 cut(s) 264
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 1 cut(s) 89
TscAI CASTG 1 cut(s) 426
TseFI GTSAC 1 cut(s) 421
Tsp45I GTSAC 1 cut(s) 421
TspDTI ATGAA 3 cut(s) 291, 397, 399
TspRI CASTG 1 cut(s) 426
VpaK11BI GGWCC 1 cut(s) 442
XmiI GTMKAC 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.