Rroxscaffold_1G00024710

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011861
Physical Location & Seq
Forward (+)
30724483 .. 30726274
1792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00024710.1

Sequence Viewer

Length: 726 bp
ATGCTTCTTGCCATTGTTGGATTTTCTGATGTATATATGCAAGCTGCTACTGACTGTGCAGACTGGGGAGGTAATGCTAGAAAGGCTTGTGAGACATTTTCTTGGGCAATATTTGATATGCTGCCACTTTACTTTGTCAATCATTATTATTATTATTATTATTATTATTATTATTATTTTTCATTGGATTGTTCTCTTTTGCAGGACCTTCTTCATAAAATATGGGTCATGGCACAGAGGGACCATCAATCAATTTGCATTCTTTCGGCTACTGGTGCTGTTTCTAAACCTGTCATACGACCTAATGCTGCTGTAGGGTATGTAAAATACGAGGGTTGTTATGATATCATCAATCTCTCTGGATCGTTTGTATACCATGCGTTGTATCCACACAAAAAATTGGGCATGATAACTATTCTACTAGCTAATCCCGATAATATGGTTTTTGGTGGCTCTGTTTTATCAGGAGCACCGCTCATAGCTGCGAGTCCTATTCAACTCATTCTCGGAGCTTTTGATCAAGATATACACGAAGAGTTGAATGCAAAAAGGAGGCCCTCAGTCGCATCTTCTACATACATGAGTGTAACAACTGGTCTAGACCTTGCAAGGATTCCTGCACAGGTGATGCCGATCCAAGCAGTAGATTGGGATGAAACTCGTATTCAATATCGACGAATATCTCCATATTTCAACAACAGTCGGGATAGGACTCGTGTTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

241

Amino Acids

27.39

Weight (kDa)

6.57

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 69 - 173 2.2e-10 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 475
AccI GTMKAC 1 cut(s) 372
AciI CCGC 1 cut(s) 473
AclWI GGATC 2 cut(s) 370, 628
AgsI TTSAA 5 cut(s) 497, 541, 668, 694, 722
AluBI AGCT 4 cut(s) 44, 425, 482, 512
AluI AGCT 4 cut(s) 44, 425, 482, 512
Alw21I GWGCWC 1 cut(s) 472
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 2 cut(s) 370, 628
AoxI GGCC 1 cut(s) 554
ApeKI GCWGC 4 cut(s) 44, 121, 308, 482
AspS9I GGNCC 3 cut(s) 205, 241, 555
AsuHPI GGTGA 1 cut(s) 637
AvaII GGWCC 2 cut(s) 205, 241
BauI CACGAG 1 cut(s) 714
Bbv12I GWGCWC 1 cut(s) 472
BbvI GCAGC 4 cut(s) 31, 108, 295, 469
BccI CCATC 1 cut(s) 252
BciVI GTATCC 1 cut(s) 396
BclI TGATCA 1 cut(s) 517
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 3 cut(s) 78, 422, 599
BfmI CTRYAG 1 cut(s) 312
BfuI GTATCC 1 cut(s) 396
BisI GCNGC 4 cut(s) 45, 122, 309, 483
BlsI GCNGC 4 cut(s) 46, 123, 310, 484
Bme18I GGWCC 2 cut(s) 205, 241
BmgT120I GGNCC 3 cut(s) 205, 241, 555
BmiI GGNNCC 1 cut(s) 242
BmrI ACTGGG 1 cut(s) 73
BmsI GCATC 2 cut(s) 575, 618
BmuI ACTGGG 1 cut(s) 73
BplI GAGNNNNNCTC 2 cut(s) 459, 491
BsaBI GATNNNNATC 1 cut(s) 632
Bse1I ACTGG 3 cut(s) 68, 277, 598
Bse8I GATNNNNATC 1 cut(s) 632
BseGI GGATG 1 cut(s) 658
BseJI GATNNNNATC 1 cut(s) 632
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 3 cut(s) 68, 277, 598
BseXI GCAGC 4 cut(s) 31, 108, 295, 469
BsgI GTGCAG 2 cut(s) 78, 603
BshFI GGCC 1 cut(s) 556
BsiHKAI GWGCWC 1 cut(s) 472
BslFI GGGAC 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 254
BsmI GAATGC 2 cut(s) 258, 547
BsnI GGCC 1 cut(s) 556
Bsp1286I GDGCHC 1 cut(s) 472
Bsp143I GATC 3 cut(s) 362, 517, 633
BspACI CCGC 1 cut(s) 473
BspANI GGCC 1 cut(s) 556
BspCNI CTCAG 1 cut(s) 572
BspLI GGNNCC 1 cut(s) 242
BspPI GGATC 2 cut(s) 370, 628
BsrBI CCGCTC 1 cut(s) 475
BsrI ACTGG 3 cut(s) 68, 277, 598
BssMI GATC 3 cut(s) 362, 517, 633
BssNAI GTATAC 1 cut(s) 373
BssSI CACGAG 1 cut(s) 714
Bst1107I GTATAC 1 cut(s) 373
Bst2BI CACGAG 1 cut(s) 714
Bst4CI ACNGT 2 cut(s) 56, 701
Bst6I CTCTTC 1 cut(s) 528
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 1 cut(s) 559
BstF5I GGATG 1 cut(s) 658
BstKTI GATC 3 cut(s) 365, 520, 636
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 3 cut(s) 362, 517, 633
BstMWI GCNNNNNNNGC 2 cut(s) 83, 275
BstSFI CTRYAG 1 cut(s) 312
BstV1I GCAGC 4 cut(s) 31, 108, 295, 469
BstZ17I GTATAC 1 cut(s) 373
BsuI GTATCC 1 cut(s) 396
BsuRI GGCC 1 cut(s) 556
BtsCI GGATG 1 cut(s) 658
Cac8I GCNNGC 1 cut(s) 42
Cfr13I GGNCC 3 cut(s) 205, 241, 555
CviAII CATG 4 cut(s) 229, 377, 406, 580
CviJI RGCY 8 cut(s) 44, 86, 269, 425, 453, 482, 512, 556
CviKI_1 RGCY 8 cut(s) 44, 86, 269, 425, 453, 482, 512, 556
DdeI CTNAG 1 cut(s) 559
DpnI GATC 3 cut(s) 364, 519, 635
DpnII GATC 3 cut(s) 362, 517, 633
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
Eco32I GATATC 1 cut(s) 346
Eco47I GGWCC 2 cut(s) 205, 241
EcoO109I RGGNCCY 2 cut(s) 205, 555
EcoRV GATATC 1 cut(s) 346
FaeI CATG 4 cut(s) 232, 380, 409, 583
FaqI GGGAC 1 cut(s) 254
FatI CATG 4 cut(s) 228, 376, 405, 579
FbaI TGATCA 1 cut(s) 517
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 4 cut(s) 45, 122, 309, 483
FokI GGATG 1 cut(s) 665
Fsp4HI GCNGC 4 cut(s) 45, 122, 309, 483
FspBI CTAG 3 cut(s) 78, 422, 599
GluI GCNGC 4 cut(s) 45, 122, 309, 483
HaeIII GGCC 1 cut(s) 556
Hin1II CATG 4 cut(s) 232, 380, 409, 583
HinfI GANTC 3 cut(s) 487, 613, 712
HphI GGTGA 1 cut(s) 637
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 2 cut(s) 28, 509
Hpy188III TCNNGA 6 cut(s) 360, 431, 465, 521, 599, 704
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 1 cut(s) 678
HpyAV CCTTC 1 cut(s) 218
HpyCH4III ACNGT 2 cut(s) 56, 701
HpyCH4V TGCA 7 cut(s) 40, 59, 202, 258, 545, 608, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 275
HpyF3I CTNAG 1 cut(s) 559
Hsp92II CATG 4 cut(s) 232, 380, 409, 583
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 3 cut(s) 362, 517, 633
LmnI GCTCC 2 cut(s) 467, 509
LpnPI CCDG 9 cut(s) 49, 188, 258, 303, 345, 450, 579, 608, 630
Lsp1109I GCAGC 4 cut(s) 31, 108, 295, 469
LweI GCATC 2 cut(s) 575, 618
MaeI CTAG 3 cut(s) 78, 422, 599
MaeIII GTNAC 1 cut(s) 586
MalI GATC 3 cut(s) 364, 519, 635
MbiI CCGCTC 1 cut(s) 475
MboI GATC 3 cut(s) 362, 517, 633
MboII GAAGA 3 cut(s) 203, 545, 561
MhlI GDGCHC 1 cut(s) 472
MluCI AATT 2 cut(s) 252, 398
MlyI GAGTC 2 cut(s) 496, 706
MnlI CCTC 5 cut(s) 62, 231, 325, 546, 568
Mva1269I GAATGC 2 cut(s) 258, 547
MwoI GCNNNNNNNGC 2 cut(s) 83, 275
NdeII GATC 3 cut(s) 362, 517, 633
NlaIII CATG 4 cut(s) 232, 380, 409, 583
NlaIV GGNNCC 1 cut(s) 242
PctI GAATGC 2 cut(s) 258, 547
PfeI GAWTC 1 cut(s) 613
PkrI GCNGC 4 cut(s) 46, 123, 310, 484
PleI GAGTC 2 cut(s) 495, 706
PpsI GAGTC 2 cut(s) 495, 706
PpuMI RGGWCCY 1 cut(s) 205
Psp5II RGGWCCY 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 242
PspPI GGNCC 3 cut(s) 205, 241, 555
PspPPI RGGWCCY 1 cut(s) 205
SatI GCNGC 4 cut(s) 45, 122, 309, 483
Sau3AI GATC 3 cut(s) 362, 517, 633
Sau96I GGNCC 3 cut(s) 205, 241, 555
SchI GAGTC 2 cut(s) 496, 706
SduI GDGCHC 1 cut(s) 472
SfaNI GCATC 2 cut(s) 575, 618
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 2 cut(s) 205, 241
Sse9I AATT 2 cut(s) 252, 398
SsiI CCGC 1 cut(s) 473
SspI AATATT 1 cut(s) 111
SspMI CTAG 3 cut(s) 78, 422, 599
TaaI ACNGT 2 cut(s) 56, 701
TaqI TCGA 1 cut(s) 673
TasI AATT 2 cut(s) 252, 398
TfiI GAWTC 1 cut(s) 613
TseI GCWGC 4 cut(s) 44, 121, 308, 482
TspDTI ATGAA 3 cut(s) 171, 203, 669
VpaK11BI GGWCC 2 cut(s) 205, 241
XbaI TCTAGA 1 cut(s) 598
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 3 cut(s) 78, 422, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.