Rh5AG363500

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011861
Physical Location & Seq
Reverse (-)
61386160 .. 61389212
3053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG363500.1

Sequence Viewer

Length: 1098 bp
ATGTCTAATCCCATGGAAGAGAAGAACATTATGGAATCTCCCCCTATCTCTCCCCTAAGCAGTGAACCAGAACCGAACAATAGTATACAGTTGGTGGGGTGTCTTCCACAGTCTGACCTTATCGCCAATGAGTTGGTGGAGTTTCCTCCACAGTCTGACCCTATCGCCGATGAGTTGGTGGAGTCTTCTCTGCAGTCTAATCCCATTGCCAATGAGTTGTTGGAGTCTCCTCCAGAGTCTGAGTCTGACCCCATCGCCGATGAGAACAATGAAGCACTATCAGCTGAAGGCGGTCAATCGGAGACAGGTGAAGTGATGATGACACCAAAGAAGAAGAGGGGGAGAACTAGGAATTATGAGGTCAATGAAGAAATGGTCTCTGATGTTCCGGCTTCCAACTCGGAGGGCTCCTCTAAGGAAGCAAAAGACCACCCCAAAGGCTTAGGCAATAAATTGCAACGCCCCGCTGCCGATGAAACTGGTGGAGCTGGAAATAACTTCATCCCTTTCGCAGTGACTGTGCAGACTGGGGAGGATCTTGTTCATAAAATATGGGTCATGGCACAGAGGGACCATCAATCAATTTGCATTCTTTCGGCTACTGGTGCTGTTTCTAAACCTGTCATACGACCTAATGCTGTTGTAGGGTATGTAAAATACGAGGGTTGTTATGATATCATCAATCTCTCTGGATCGTTTGTATACCATGCAATGTATCCACACAAAAAATTGGGCATGATAACTATTCTACTGGCTAATCCCGATAATACGGTTTTTGGTGGCTCTGTTTTATCAGGAGCACCGCTCATAGCTGCGAGTCCTATTCAACTCATTCTCGGAGCTTTTGATCAAGACATACATGAAGATCAGTTGAATGAGAAAAGGAGGCCCTCAGTCGCATCTTCTACATACACAAGTGTAACAGTTGGTCCTGACCTTGCAAGGATTCCTGCACAAGTGATGCCGATCCAAGTAGTAGATCGGGATGAAACTCGTATTCAATATCGACGAATATCTCCATATTTCAACAACAGTCGGGATGGGACTTGTGTTCAATATCGACCAATACCTCCATATTTCAACAACAGTCTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

365

Amino Acids

39.76

Weight (kDa)

4.73

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 169 - 283 4.1e-14 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 805
AccI GTMKAC 2 cut(s) 85, 702
AciI CCGC 3 cut(s) 291, 465, 803
AclWI GGATC 3 cut(s) 543, 700, 961
AcuI CTGAAG 1 cut(s) 306
AgsI TTSAA 6 cut(s) 827, 874, 1001, 1027, 1055, 1081
AluBI AGCT 4 cut(s) 284, 488, 812, 842
AluI AGCT 4 cut(s) 284, 488, 812, 842
Alw21I GWGCWC 1 cut(s) 802
Alw26I GTCTC 3 cut(s) 231, 296, 382
AlwI GGATC 3 cut(s) 543, 700, 961
AlwNI CAGNNNCTG 2 cut(s) 239, 518
AoxI GGCC 1 cut(s) 887
ApeKI GCWGC 2 cut(s) 467, 812
AspS9I GGNCC 3 cut(s) 571, 888, 929
AsuHPI GGTGA 1 cut(s) 320
AvaII GGWCC 2 cut(s) 571, 929
BanII GRGCYC 1 cut(s) 410
BbsI GAAGAC 3 cut(s) 95, 177, 1082
Bbv12I GWGCWC 1 cut(s) 802
BbvI GCAGC 2 cut(s) 454, 799
BccI CCATC 3 cut(s) 260, 582, 1034
BciVI GTATCC 1 cut(s) 726
BclI TGATCA 1 cut(s) 847
BcoDI GTCTC 3 cut(s) 231, 296, 382
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 1 cut(s) 191
BfuI GTATCC 1 cut(s) 726
BisI GCNGC 2 cut(s) 468, 813
BlsI GCNGC 2 cut(s) 469, 814
Bme18I GGWCC 2 cut(s) 571, 929
BmgT120I GGNCC 3 cut(s) 571, 888, 929
BmiI GGNNCC 2 cut(s) 409, 572
BmrI ACTGGG 1 cut(s) 537
BmsI GCATC 2 cut(s) 908, 951
BmuI ACTGGG 1 cut(s) 537
BpiI GAAGAC 3 cut(s) 95, 177, 1082
BplI GAGNNNNNCTC 4 cut(s) 395, 427, 789, 821
BpmI CTGGAG 1 cut(s) 216
Bpu10I CCTNAGC 2 cut(s) 56, 442
BsaBI GATNNNNATC 1 cut(s) 965
BsaI GGTCTC 1 cut(s) 382
BsaJI CCNNGG 1 cut(s) 12
Bse1I ACTGG 4 cut(s) 484, 532, 607, 756
Bse3DI GCAATG 2 cut(s) 204, 717
Bse8I GATNNNNATC 1 cut(s) 965
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 3 cut(s) 501, 991, 1045
BseJI GATNNNNATC 1 cut(s) 965
BseMI GCAATG 2 cut(s) 204, 717
BseMII CTCAG 2 cut(s) 231, 906
BseNI ACTGG 4 cut(s) 484, 532, 607, 756
BseRI GAGGAG 2 cut(s) 219, 400
BseXI GCAGC 2 cut(s) 454, 799
BsgI GTGCAG 2 cut(s) 542, 936
BshFI GGCC 1 cut(s) 889
BsiHKAI GWGCWC 1 cut(s) 802
BsiSI CCGG 1 cut(s) 389
BslFI GGGAC 2 cut(s) 584, 1057
BsmAI GTCTC 3 cut(s) 231, 296, 382
BsmFI GGGAC 2 cut(s) 584, 1057
BsmI GAATGC 1 cut(s) 588
BsnI GGCC 1 cut(s) 889
Bso31I GGTCTC 1 cut(s) 382
Bsp1286I GDGCHC 2 cut(s) 410, 802
Bsp143I GATC 6 cut(s) 535, 692, 847, 865, 966, 979
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 3 cut(s) 291, 465, 803
BspANI GGCC 1 cut(s) 889
BspCNI CTCAG 2 cut(s) 232, 905
BspLI GGNNCC 2 cut(s) 409, 572
BspMAI CTGCAG 1 cut(s) 195
BspPI GGATC 3 cut(s) 543, 700, 961
BspTNI GGTCTC 1 cut(s) 382
BsrBI CCGCTC 1 cut(s) 805
BsrDI GCAATG 2 cut(s) 204, 717
BsrI ACTGG 4 cut(s) 484, 532, 607, 756
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 6 cut(s) 535, 692, 847, 865, 966, 979
BssNAI GTATAC 2 cut(s) 86, 703
BssT1I CCWWGG 1 cut(s) 12
Bst1107I GTATAC 2 cut(s) 86, 703
Bst4CI ACNGT 8 cut(s) 90, 111, 153, 520, 772, 925, 1034, 1088
Bst6I CTCTTC 2 cut(s) 12, 329
BstDEI CTNAG 6 cut(s) 56, 240, 414, 442, 892, 1095
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 3 cut(s) 501, 991, 1045
BstKTI GATC 6 cut(s) 538, 695, 850, 868, 969, 982
BstMAI GTCTC 3 cut(s) 231, 296, 382
BstMBI GATC 6 cut(s) 535, 692, 847, 865, 966, 979
BstMWI GCNNNNNNNGC 2 cut(s) 281, 605
BstSFI CTRYAG 1 cut(s) 191
BstV1I GCAGC 2 cut(s) 454, 799
BstV2I GAAGAC 3 cut(s) 95, 177, 1082
BstX2I RGATCY 1 cut(s) 535
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 1 cut(s) 535
BstZ17I GTATAC 2 cut(s) 86, 703
BsuI GTATCC 1 cut(s) 726
BsuRI GGCC 1 cut(s) 889
BtgI CCRYGG 1 cut(s) 12
BtgZI GCGATG 1 cut(s) 238
BtsCI GGATG 3 cut(s) 501, 991, 1045
BtsI GCAGTG 2 cut(s) 67, 519
BtsIMutI CAGTG 2 cut(s) 67, 519
CaiI CAGNNNCTG 2 cut(s) 239, 518
Cfr13I GGNCC 3 cut(s) 571, 888, 929
CviAII CATG 5 cut(s) 13, 559, 707, 736, 860
DdeI CTNAG 6 cut(s) 56, 240, 414, 442, 892, 1095
DpnI GATC 6 cut(s) 537, 694, 849, 867, 968, 981
DpnII GATC 6 cut(s) 535, 692, 847, 865, 966, 979
Eam1104I CTCTTC 2 cut(s) 12, 329
EarI CTCTTC 2 cut(s) 12, 329
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 410
Eco31I GGTCTC 1 cut(s) 382
Eco32I GATATC 1 cut(s) 676
Eco47I GGWCC 2 cut(s) 571, 929
Eco57I CTGAAG 1 cut(s) 306
EcoO109I RGGNCCY 1 cut(s) 888
EcoRV GATATC 1 cut(s) 676
EcoT14I CCWWGG 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 410
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 5 cut(s) 16, 562, 710, 739, 863
FaqI GGGAC 2 cut(s) 584, 1057
FatI CATG 5 cut(s) 12, 558, 706, 735, 859
FauI CCCGC 1 cut(s) 472
FbaI TGATCA 1 cut(s) 847
FblI GTMKAC 2 cut(s) 85, 702
Fnu4HI GCNGC 2 cut(s) 468, 813
FokI GGATG 3 cut(s) 488, 998, 1052
FriOI GRGCYC 1 cut(s) 410
Fsp4HI GCNGC 2 cut(s) 468, 813
FspBI CTAG 1 cut(s) 348
GluI GCNGC 2 cut(s) 468, 813
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 1 cut(s) 889
HapII CCGG 1 cut(s) 389
Hin1II CATG 5 cut(s) 16, 562, 710, 739, 863
HinfI GANTC 7 cut(s) 35, 182, 224, 236, 242, 817, 946
HpaII CCGG 1 cut(s) 389
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 3 cut(s) 65, 86, 703
Hpy188I TCNGA 8 cut(s) 115, 157, 241, 247, 301, 382, 403, 839
Hpy188III TCNNGA 8 cut(s) 233, 690, 761, 795, 851, 932, 983, 1037
Hpy8I GTNNAC 3 cut(s) 65, 86, 703
Hpy99I CGWCG 1 cut(s) 1011
HpyAV CCTTC 1 cut(s) 281
HpyCH4III ACNGT 8 cut(s) 90, 111, 153, 520, 772, 925, 1034, 1088
HpyCH4V TGCA 7 cut(s) 193, 457, 523, 588, 710, 941, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 281, 605
HpyF3I CTNAG 6 cut(s) 56, 240, 414, 442, 892, 1095
Hsp92II CATG 5 cut(s) 16, 562, 710, 739, 863
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 6 cut(s) 535, 692, 847, 865, 966, 979
LmnI GCTCC 4 cut(s) 413, 485, 797, 839
Lsp1109I GCAGC 2 cut(s) 454, 799
LweI GCATC 2 cut(s) 908, 951
MaeI CTAG 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 514, 919
MalI GATC 6 cut(s) 537, 694, 849, 867, 968, 981
MbiI CCGCTC 1 cut(s) 805
MboI GATC 6 cut(s) 535, 692, 847, 865, 966, 979
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 2 cut(s) 410, 802
MluCI AATT 4 cut(s) 352, 452, 582, 728
MlyI GAGTC 5 cut(s) 191, 233, 245, 251, 826
MmeI TCCRAC 2 cut(s) 201, 420
MspA1I CMGCKG 2 cut(s) 284, 467
MspI CCGG 1 cut(s) 389
Mva1269I GAATGC 1 cut(s) 588
MwoI GCNNNNNNNGC 2 cut(s) 281, 605
NcoI CCATGG 1 cut(s) 12
NdeII GATC 6 cut(s) 535, 692, 847, 865, 966, 979
NlaIII CATG 5 cut(s) 16, 562, 710, 739, 863
NlaIV GGNNCC 2 cut(s) 409, 572
NmuCI GTSAC 1 cut(s) 514
PctI GAATGC 1 cut(s) 588
PfeI GAWTC 2 cut(s) 35, 946
PkrI GCNGC 2 cut(s) 469, 814
PleI GAGTC 5 cut(s) 190, 232, 244, 250, 825
PpsI GAGTC 5 cut(s) 190, 232, 244, 250, 825
PspN4I GGNNCC 2 cut(s) 409, 572
PspPI GGNCC 3 cut(s) 571, 888, 929
PstI CTGCAG 1 cut(s) 195
PstNI CAGNNNCTG 2 cut(s) 239, 518
PsuI RGATCY 1 cut(s) 535
PvuII CAGCTG 1 cut(s) 284
SatI GCNGC 2 cut(s) 468, 813
Sau3AI GATC 6 cut(s) 535, 692, 847, 865, 966, 979
Sau96I GGNCC 3 cut(s) 571, 888, 929
SchI GAGTC 5 cut(s) 191, 233, 245, 251, 826
SduI GDGCHC 2 cut(s) 410, 802
SfaNI GCATC 2 cut(s) 908, 951
SfcI CTRYAG 1 cut(s) 191
SinI GGWCC 2 cut(s) 571, 929
Sse9I AATT 4 cut(s) 352, 452, 582, 728
SsiI CCGC 3 cut(s) 291, 465, 803
SspMI CTAG 1 cut(s) 348
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 8 cut(s) 90, 111, 153, 520, 772, 925, 1034, 1088
TaqI TCGA 2 cut(s) 1006, 1060
TasI AATT 4 cut(s) 352, 452, 582, 728
TfiI GAWTC 2 cut(s) 35, 946
TscAI CASTG 2 cut(s) 67, 519
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 2 cut(s) 467, 812
Tsp45I GTSAC 1 cut(s) 514
TspDTI ATGAA 7 cut(s) 285, 381, 489, 490, 533, 876, 1002
TspRI CASTG 2 cut(s) 67, 519
VpaK11BI GGWCC 2 cut(s) 571, 929
XcmI CCANNNNNNNNNTGG 2 cut(s) 133, 217
XmiI GTMKAC 2 cut(s) 85, 702
XspI CTAG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.