RLG00000034998

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011861
Physical Location & Seq
Reverse (-)
58873116 .. 58876207
3092 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034998

Sequence Viewer

Length: 999 bp
ATGGAAGAGAAGAACATTATGGAATCTCCCCCTATCTCTCTCCTAAGCAGTGAACCGGATTCGAACCACAGTAAACAGTTGTCTGACCTTATCGCCAATGAGTTGGTGGTGTTTCCTCCACTGTCTGACTCCATCGCCGATGAGTTGGTGGAGTCTTCTCTGCAGTCTGATCCCATTGCCAATGAGTTGTTGGAGTCTCCTCCACAGTCTGAGTCTGACCCCATCACCGATGAGAACAATGAAGCACTATCAGCTGAAGAAAGCGGTCAATCGGAGGCAGGTGAAGTGATGATGACACCCAAGAAGAAGAGGGGGAGACCCAGGAATTACGAGGTCAATGAAGAAATGGTATCTGATGTTCTGGCTTCCAACTCGGAGTGCTTCTCTAAGAAAACAAAAGGCCACCCCGAAGGCTCAGGCAATAAATTGCAACGCCCCGTTGCCGATGGTTTTTTTGTTGTAGAAACTGGTGGAGCTGGAAATAACTTCATCCCTTTCGCAGTGACTGTGCAGACTGGGGAGGACCTTCTTCATAAAATATGGGTAATGGCACAGAGGGACCATCAATCAATTTGTATTCTTTCGGCTACTGGTGCTGTTTCTAAACCTGTCATACGCCCTAATGCTGCTGTAGGGTATGTAAAATACGAGGGTTGTTATGATATAATCAATCTCTCTGGATCGTTTGTATACCATGCAATTTATCCACACAAAAAATTGGGCATGATAACTATTCTACTGGCTAATCCCGATAATACGGTTTTTGGTGGCTCTGTTTTATCAGGAGCACCGCTCATAGCTACGAGTCCTATTCAAAGTGTAACAGCTGGTCCAGACCTTGCAACGATTCCTGCACAAGTGATGCCGATCCAAGCAGTAGATCGGGATGAAACTTGTATTCAATATCGACGAATATCTCCATATTTCAACAATAGTCGGGATGGGACTTGTGTTCAATATCGACCAATACCTCCATATTTCAACAACAGTCTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

333

Amino Acids

36.05

Weight (kDa)

4.74

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 165 - 272 5.3e-12 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 269
AccBSI CCGCTC 1 cut(s) 793
AccI GTMKAC 1 cut(s) 690
AciI CCGC 2 cut(s) 264, 791
AclWI GGATC 3 cut(s) 164, 688, 862
AcuI CTGAAG 1 cut(s) 276
AgsI TTSAA 5 cut(s) 815, 902, 928, 956, 982
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 4 cut(s) 254, 476, 800, 827
AluI AGCT 4 cut(s) 254, 476, 800, 827
Alw21I GWGCWC 1 cut(s) 790
Alw26I GTCTC 2 cut(s) 201, 310
AlwI GGATC 3 cut(s) 164, 688, 862
AlwNI CAGNNNCTG 1 cut(s) 506
AoxI GGCC 1 cut(s) 400
ApeKI GCWGC 1 cut(s) 626
AspS9I GGNCC 3 cut(s) 523, 559, 830
AsuHPI GGTGA 2 cut(s) 217, 293
AsuII TTCGAA 1 cut(s) 62
AvaII GGWCC 3 cut(s) 523, 559, 830
BbsI GAAGAC 2 cut(s) 147, 983
Bbv12I GWGCWC 1 cut(s) 790
BbvI GCAGC 1 cut(s) 613
BccI CCATC 5 cut(s) 140, 230, 440, 570, 935
BciT130I CCWGG 1 cut(s) 322
BcoDI GTCTC 2 cut(s) 201, 310
BfmI CTRYAG 2 cut(s) 161, 630
BfuAI ACCTGC 1 cut(s) 269
BisI GCNGC 1 cut(s) 627
BlsI GCNGC 1 cut(s) 628
Bme1390I CCNGG 1 cut(s) 322
Bme18I GGWCC 3 cut(s) 523, 559, 830
BmgT120I GGNCC 3 cut(s) 523, 559, 830
BmiI GGNNCC 1 cut(s) 560
BmrFI CCNGG 1 cut(s) 322
BmrI ACTGGG 1 cut(s) 525
BmsI GCATC 1 cut(s) 852
BmuI ACTGGG 1 cut(s) 525
BpiI GAAGAC 2 cut(s) 147, 983
BplI GAGNNNNNCTC 4 cut(s) 368, 400, 777, 809
Bpu10I CCTNAGC 2 cut(s) 44, 415
Bpu14I TTCGAA 1 cut(s) 62
BsaBI GATNNNNATC 1 cut(s) 866
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 1 cut(s) 320
BsaWI WCCGGW 1 cut(s) 55
Bse1I ACTGG 4 cut(s) 472, 520, 595, 744
Bse3DI GCAATG 1 cut(s) 174
Bse8I GATNNNNATC 1 cut(s) 866
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 1 cut(s) 320
BseGI GGATG 3 cut(s) 489, 892, 946
BseJI GATNNNNATC 1 cut(s) 866
BseMI GCAATG 1 cut(s) 174
BseMII CTCAG 2 cut(s) 201, 429
BseNI ACTGG 4 cut(s) 472, 520, 595, 744
BseRI GAGGAG 1 cut(s) 189
BseXI GCAGC 1 cut(s) 613
BsgI GTGCAG 2 cut(s) 530, 837
BshFI GGCC 1 cut(s) 402
BsiHKAI GWGCWC 1 cut(s) 790
BsiSI CCGG 1 cut(s) 56
BslFI GGGAC 2 cut(s) 572, 958
BsmAI GTCTC 2 cut(s) 201, 310
BsmFI GGGAC 2 cut(s) 572, 958
BsnI GGCC 1 cut(s) 402
Bso31I GGTCTC 1 cut(s) 310
Bsp119I TTCGAA 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 790
Bsp143I GATC 4 cut(s) 169, 680, 867, 880
BspACI CCGC 2 cut(s) 264, 791
BspANI GGCC 1 cut(s) 402
BspCNI CTCAG 2 cut(s) 202, 428
BspLI GGNNCC 1 cut(s) 560
BspMAI CTGCAG 1 cut(s) 165
BspMI ACCTGC 1 cut(s) 269
BspPI GGATC 3 cut(s) 164, 688, 862
BspT104I TTCGAA 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 310
BsrBI CCGCTC 1 cut(s) 793
BsrDI GCAATG 1 cut(s) 174
BsrI ACTGG 4 cut(s) 472, 520, 595, 744
BssECI CCNNGG 1 cut(s) 320
BssMI GATC 4 cut(s) 169, 680, 867, 880
BssNAI GTATAC 1 cut(s) 691
Bst1107I GTATAC 1 cut(s) 691
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 7 cut(s) 71, 78, 123, 207, 508, 760, 989
Bst6I CTCTTC 1 cut(s) 302
BstBI TTCGAA 1 cut(s) 62
BstDEI CTNAG 5 cut(s) 44, 210, 387, 415, 996
BstF5I GGATG 3 cut(s) 489, 892, 946
BstKTI GATC 4 cut(s) 172, 683, 870, 883
BstMAI GTCTC 2 cut(s) 201, 310
BstMBI GATC 4 cut(s) 169, 680, 867, 880
BstMWI GCNNNNNNNGC 2 cut(s) 251, 593
BstNI CCWGG 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 320
BstSFI CTRYAG 2 cut(s) 161, 630
BstV1I GCAGC 1 cut(s) 613
BstV2I GAAGAC 2 cut(s) 147, 983
BstXI CCANNNNNNTGG 1 cut(s) 103
BstZ17I GTATAC 1 cut(s) 691
BsuRI GGCC 1 cut(s) 402
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 3 cut(s) 489, 892, 946
BtsI GCAGTG 2 cut(s) 55, 507
BtsIMutI CAGTG 3 cut(s) 55, 119, 507
BveI ACCTGC 1 cut(s) 269
CaiI CAGNNNCTG 1 cut(s) 506
Cfr13I GGNCC 3 cut(s) 523, 559, 830
CviAII CATG 2 cut(s) 695, 724
DdeI CTNAG 5 cut(s) 44, 210, 387, 415, 996
DpnI GATC 4 cut(s) 171, 682, 869, 882
DpnII GATC 4 cut(s) 169, 680, 867, 880
Eam1104I CTCTTC 1 cut(s) 302
EarI CTCTTC 1 cut(s) 302
Eco31I GGTCTC 1 cut(s) 310
Eco47I GGWCC 3 cut(s) 523, 559, 830
Eco57I CTGAAG 1 cut(s) 276
EcoO109I RGGNCCY 1 cut(s) 523
EcoRII CCWGG 1 cut(s) 320
FaeI CATG 2 cut(s) 698, 727
FaqI GGGAC 2 cut(s) 572, 958
FatI CATG 2 cut(s) 694, 723
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 1 cut(s) 627
FokI GGATG 3 cut(s) 476, 899, 953
Fsp4HI GCNGC 1 cut(s) 627
GluI GCNGC 1 cut(s) 627
HaeIII GGCC 1 cut(s) 402
HapII CCGG 1 cut(s) 56
Hin1II CATG 2 cut(s) 698, 727
HinfI GANTC 8 cut(s) 23, 59, 128, 152, 194, 212, 805, 847
HpaII CCGG 1 cut(s) 56
HphI GGTGA 2 cut(s) 217, 293
Hpy166II GTNNAC 3 cut(s) 53, 74, 691
Hpy188I TCNGA 8 cut(s) 85, 127, 169, 211, 217, 274, 355, 376
Hpy188III TCNNGA 6 cut(s) 678, 749, 783, 833, 884, 938
Hpy8I GTNNAC 3 cut(s) 53, 74, 691
Hpy99I CGWCG 1 cut(s) 912
HpyAV CCTTC 2 cut(s) 404, 536
HpyCH4III ACNGT 7 cut(s) 71, 78, 123, 207, 508, 760, 989
HpyCH4V TGCA 6 cut(s) 163, 430, 511, 698, 842, 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 593
HpyF3I CTNAG 5 cut(s) 44, 210, 387, 415, 996
Hsp92II CATG 2 cut(s) 698, 727
Kzo9I GATC 4 cut(s) 169, 680, 867, 880
LmnI GCTCC 2 cut(s) 473, 785
Lsp1109I GCAGC 1 cut(s) 613
LweI GCATC 1 cut(s) 852
MaeIII GTNAC 2 cut(s) 502, 820
MalI GATC 4 cut(s) 171, 682, 869, 882
MbiI CCGCTC 1 cut(s) 793
MboI GATC 4 cut(s) 169, 680, 867, 880
MboII GAAGA 9 cut(s) 17, 22, 147, 269, 316, 319, 353, 521, 983
MhlI GDGCHC 1 cut(s) 790
MluCI AATT 5 cut(s) 325, 425, 570, 699, 716
MlyI GAGTC 5 cut(s) 122, 161, 203, 221, 814
MmeI TCCRAC 2 cut(s) 171, 393
MnlI CCTC 9 cut(s) 126, 210, 268, 303, 325, 514, 549, 643, 981
MspA1I CMGCKG 2 cut(s) 254, 827
MspI CCGG 1 cut(s) 56
MspR9I CCNGG 1 cut(s) 322
MvaI CCWGG 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 251, 593
NdeII GATC 4 cut(s) 169, 680, 867, 880
NlaIII CATG 2 cut(s) 698, 727
NlaIV GGNNCC 1 cut(s) 560
NmuCI GTSAC 1 cut(s) 502
NspV TTCGAA 1 cut(s) 62
PaqCI CACCTGC 1 cut(s) 269
PfeI GAWTC 3 cut(s) 23, 59, 847
PkrI GCNGC 1 cut(s) 628
PleI GAGTC 5 cut(s) 122, 160, 202, 220, 813
PpsI GAGTC 5 cut(s) 122, 160, 202, 220, 813
PpuMI RGGWCCY 1 cut(s) 523
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 1 cut(s) 560
PspPI GGNCC 3 cut(s) 523, 559, 830
PspPPI RGGWCCY 1 cut(s) 523
PsrI GAACNNNNNNTAC 2 cut(s) 342, 374
PstI CTGCAG 1 cut(s) 165
PstNI CAGNNNCTG 1 cut(s) 506
PvuII CAGCTG 2 cut(s) 254, 827
SatI GCNGC 1 cut(s) 627
Sau3AI GATC 4 cut(s) 169, 680, 867, 880
Sau96I GGNCC 3 cut(s) 523, 559, 830
SchI GAGTC 5 cut(s) 122, 161, 203, 221, 814
ScrFI CCNGG 1 cut(s) 322
SduI GDGCHC 1 cut(s) 790
SfaNI GCATC 1 cut(s) 852
SfcI CTRYAG 2 cut(s) 161, 630
SfuI TTCGAA 1 cut(s) 62
SinI GGWCC 3 cut(s) 523, 559, 830
Sse9I AATT 5 cut(s) 325, 425, 570, 699, 716
SsiI CCGC 2 cut(s) 264, 791
StyD4I CCNGG 1 cut(s) 320
TaaI ACNGT 7 cut(s) 71, 78, 123, 207, 508, 760, 989
TaqI TCGA 3 cut(s) 62, 907, 961
TasI AATT 5 cut(s) 325, 425, 570, 699, 716
TfiI GAWTC 3 cut(s) 23, 59, 847
TscAI CASTG 3 cut(s) 55, 126, 507
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 1 cut(s) 626
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 5 cut(s) 255, 354, 478, 521, 903
TspRI CASTG 3 cut(s) 55, 126, 507
VpaK11BI GGWCC 3 cut(s) 523, 559, 830
XcmI CCANNNNNNNNNTGG 2 cut(s) 103, 187
XmiI GTMKAC 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.