Rh5DG388700

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011861
Physical Location & Seq
Reverse (-)
60453515 .. 60456523
3009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG388700.1

Sequence Viewer

Length: 1065 bp
ATGGAATCTCCCCCTATCTCTCCCCTAAGCAGTGAACCAGAACCGAACAATAGTAAACAGTTGGTGGAGTTTCCTCCACAATCTGACCTTATCGCCAATGAGTTGGTGGAGTTTCCTCCACAATCTGACCCTATCGCCGATGAGTTGGTGGAGTCTTCTCTGCAGTCTGATCCCATTGCCAATGAGTTGTTGGAGTCTCCTTCACAGTCTGAGTCTGACCCCATCGCCTATGAGAACAATGAAGCACTATCAGCTGAAGGCGGTCAATCGGAGGCAGGTGAAGTGATGATGACACCTAAGAAGAAGAGGGGGAGACCCAGGAATTATGAGGTCAATGAAGAAATGGTCTCTGATGTTCCGGCTTCCAACTCGGAGGGCTCCTCTAAGAAAGCAAAAGACCACCCCAAAGGCTCAGGCAATAAATTGCAATGCCCCGCTGCCGATGAAACTGGTGGAGTTGGAAATAACTTCATCCCTTTCGCAGTGACTGTGCAGACTGGGGAGGACCTTGTTCATAAAATATGGGTCATGGCACAGAGGGACCATCAATCAATTTGCATTCTTTCGGCTACTGGTGCTGTTTCTAAACCTGTCATACGACCTAATGCTGCTGTAGGGTATGCAAAATACGAGGGTTGTTATGATATCATCAATCTCTCTGGATCGTTTGTATACCATGCAATGTATCCACACAAAAAATTGGGCATGATAACTATTCTACTGGCTAATCCCGATAATACGGTTTTTGGTGGCTCTGTTTTATCAGGAGCACCACTCATAGCTGCGAGTCCTATTCAACTCATTCTCGGAGCTTTTGATCAAGACATACATGAAGAGTTGAATGCGAAAAGGAGGCCCTCAGTCGCATCTTCTACATACACGAGTGTAACAGCTGGTCCTGACCTTGCAAGGATTCCTGCACAAGTGATGCCGATCCAAGAAGTAGATCGGGATGAAACTCGTATTCAATATCGACGAATATCTCCATATTTCAACAACAGTCGGGATGGGACTTGTGTTCAATATCGACCAATACCTCCATATTTCAACAACAATCTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

354

Amino Acids

38.44

Weight (kDa)

4.84

Isoelectric Point (pI)

54.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 159 - 273 2.5e-14 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 266
Acc36I ACCTGC 1 cut(s) 266
AccI GTMKAC 1 cut(s) 672
AciI CCGC 2 cut(s) 261, 435
AclWI GGATC 3 cut(s) 164, 670, 928
AcuI CTGAAG 1 cut(s) 276
AgsI TTSAA 6 cut(s) 797, 841, 968, 994, 1022, 1048
AjnI CCWGG 1 cut(s) 317
AluBI AGCT 4 cut(s) 254, 782, 812, 893
AluI AGCT 4 cut(s) 254, 782, 812, 893
Alw21I GWGCWC 1 cut(s) 772
Alw26I GTCTC 3 cut(s) 201, 307, 352
AlwI GGATC 3 cut(s) 164, 670, 928
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 1 cut(s) 854
ApeKI GCWGC 3 cut(s) 437, 608, 782
AspS9I GGNCC 4 cut(s) 505, 541, 855, 896
AsuHPI GGTGA 1 cut(s) 290
AvaII GGWCC 3 cut(s) 505, 541, 896
BanII GRGCYC 1 cut(s) 380
BauI CACGAG 1 cut(s) 880
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 772
BbvI GCAGC 3 cut(s) 424, 595, 769
BccI CCATC 3 cut(s) 230, 552, 1001
BciT130I CCWGG 1 cut(s) 319
BciVI GTATCC 1 cut(s) 696
BclI TGATCA 1 cut(s) 817
BcoDI GTCTC 3 cut(s) 201, 307, 352
BfmI CTRYAG 2 cut(s) 161, 612
BfuAI ACCTGC 1 cut(s) 266
BfuI GTATCC 1 cut(s) 696
BisI GCNGC 3 cut(s) 438, 609, 783
BlsI GCNGC 3 cut(s) 439, 610, 784
Bme1390I CCNGG 1 cut(s) 319
Bme18I GGWCC 3 cut(s) 505, 541, 896
BmgT120I GGNCC 4 cut(s) 505, 541, 855, 896
BmiI GGNNCC 2 cut(s) 379, 542
BmrFI CCNGG 1 cut(s) 319
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 2 cut(s) 875, 918
BmuI ACTGGG 1 cut(s) 507
BpiI GAAGAC 1 cut(s) 147
BplI GAGNNNNNCTC 4 cut(s) 365, 397, 759, 791
Bpu10I CCTNAGC 2 cut(s) 26, 412
BsaBI GATNNNNATC 1 cut(s) 932
BsaI GGTCTC 2 cut(s) 307, 352
BsaJI CCNNGG 1 cut(s) 317
Bse1I ACTGG 4 cut(s) 454, 502, 577, 726
Bse3DI GCAATG 3 cut(s) 174, 434, 687
Bse8I GATNNNNATC 1 cut(s) 932
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 1 cut(s) 317
BseGI GGATG 3 cut(s) 471, 958, 1012
BseJI GATNNNNATC 1 cut(s) 932
BseMI GCAATG 3 cut(s) 174, 434, 687
BseMII CTCAG 3 cut(s) 201, 426, 873
BseNI ACTGG 4 cut(s) 454, 502, 577, 726
BseRI GAGGAG 1 cut(s) 370
BseXI GCAGC 3 cut(s) 424, 595, 769
BsgI GTGCAG 2 cut(s) 512, 903
BshFI GGCC 1 cut(s) 856
BsiHKAI GWGCWC 1 cut(s) 772
BsiSI CCGG 1 cut(s) 359
BslFI GGGAC 2 cut(s) 554, 1024
BsmAI GTCTC 3 cut(s) 201, 307, 352
BsmFI GGGAC 2 cut(s) 554, 1024
BsmI GAATGC 2 cut(s) 558, 847
BsnI GGCC 1 cut(s) 856
Bso31I GGTCTC 2 cut(s) 307, 352
Bsp1286I GDGCHC 2 cut(s) 380, 772
Bsp143I GATC 5 cut(s) 169, 662, 817, 933, 946
BspACI CCGC 2 cut(s) 261, 435
BspANI GGCC 1 cut(s) 856
BspCNI CTCAG 3 cut(s) 202, 425, 872
BspLI GGNNCC 2 cut(s) 379, 542
BspMAI CTGCAG 1 cut(s) 165
BspMI ACCTGC 1 cut(s) 266
BspPI GGATC 3 cut(s) 164, 670, 928
BspTNI GGTCTC 2 cut(s) 307, 352
BsrDI GCAATG 3 cut(s) 174, 434, 687
BsrI ACTGG 4 cut(s) 454, 502, 577, 726
BssECI CCNNGG 1 cut(s) 317
BssMI GATC 5 cut(s) 169, 662, 817, 933, 946
BssNAI GTATAC 1 cut(s) 673
BssSI CACGAG 1 cut(s) 880
Bst1107I GTATAC 1 cut(s) 673
Bst2BI CACGAG 1 cut(s) 880
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 5 cut(s) 60, 207, 490, 742, 1001
Bst6I CTCTTC 2 cut(s) 299, 828
BstDEI CTNAG 7 cut(s) 26, 210, 297, 384, 412, 859, 1062
BstF5I GGATG 3 cut(s) 471, 958, 1012
BstKTI GATC 5 cut(s) 172, 665, 820, 936, 949
BstMAI GTCTC 3 cut(s) 201, 307, 352
BstMBI GATC 5 cut(s) 169, 662, 817, 933, 946
BstMWI GCNNNNNNNGC 2 cut(s) 251, 575
BstNI CCWGG 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 317
BstSFI CTRYAG 2 cut(s) 161, 612
BstV1I GCAGC 3 cut(s) 424, 595, 769
BstV2I GAAGAC 1 cut(s) 147
BstXI CCANNNNNNTGG 1 cut(s) 103
BstZ17I GTATAC 1 cut(s) 673
BsuI GTATCC 1 cut(s) 696
BsuRI GGCC 1 cut(s) 856
BtgZI GCGATG 1 cut(s) 208
BtsCI GGATG 3 cut(s) 471, 958, 1012
BtsI GCAGTG 2 cut(s) 37, 489
BtsIMutI CAGTG 2 cut(s) 37, 489
BveI ACCTGC 1 cut(s) 266
CaiI CAGNNNCTG 1 cut(s) 488
Cfr13I GGNCC 4 cut(s) 505, 541, 855, 896
CviAII CATG 4 cut(s) 529, 677, 706, 830
DdeI CTNAG 7 cut(s) 26, 210, 297, 384, 412, 859, 1062
DpnI GATC 5 cut(s) 171, 664, 819, 935, 948
DpnII GATC 5 cut(s) 169, 662, 817, 933, 946
Eam1104I CTCTTC 2 cut(s) 299, 828
EarI CTCTTC 2 cut(s) 299, 828
Eco24I GRGCYC 1 cut(s) 380
Eco31I GGTCTC 2 cut(s) 307, 352
Eco32I GATATC 1 cut(s) 646
Eco47I GGWCC 3 cut(s) 505, 541, 896
Eco57I CTGAAG 1 cut(s) 276
EcoO109I RGGNCCY 2 cut(s) 505, 855
EcoRII CCWGG 1 cut(s) 317
EcoRV GATATC 1 cut(s) 646
EcoT38I GRGCYC 1 cut(s) 380
FaeI CATG 4 cut(s) 532, 680, 709, 833
FaqI GGGAC 2 cut(s) 554, 1024
FatI CATG 4 cut(s) 528, 676, 705, 829
FauI CCCGC 1 cut(s) 442
FbaI TGATCA 1 cut(s) 817
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 3 cut(s) 438, 609, 783
FokI GGATG 3 cut(s) 458, 965, 1019
FriOI GRGCYC 1 cut(s) 380
Fsp4HI GCNGC 3 cut(s) 438, 609, 783
GluI GCNGC 3 cut(s) 438, 609, 783
HaeIII GGCC 1 cut(s) 856
HapII CCGG 1 cut(s) 359
Hin1II CATG 4 cut(s) 532, 680, 709, 833
HinfI GANTC 6 cut(s) 5, 152, 194, 212, 787, 913
HpaII CCGG 1 cut(s) 359
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 3 cut(s) 35, 56, 673
Hpy188I TCNGA 9 cut(s) 85, 127, 169, 211, 217, 271, 352, 373, 809
Hpy188III TCNNGA 7 cut(s) 660, 731, 765, 821, 899, 950, 1004
Hpy8I GTNNAC 3 cut(s) 35, 56, 673
Hpy99I CGWCG 1 cut(s) 978
HpyAV CCTTC 2 cut(s) 210, 251
HpyCH4III ACNGT 5 cut(s) 60, 207, 490, 742, 1001
HpyCH4V TGCA 8 cut(s) 163, 427, 493, 558, 623, 680, 908, 920
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 575
HpyF3I CTNAG 7 cut(s) 26, 210, 297, 384, 412, 859, 1062
Hsp92II CATG 4 cut(s) 532, 680, 709, 833
Ksp22I TGATCA 1 cut(s) 817
Kzo9I GATC 5 cut(s) 169, 662, 817, 933, 946
LmnI GCTCC 3 cut(s) 383, 767, 809
Lsp1109I GCAGC 3 cut(s) 424, 595, 769
LweI GCATC 2 cut(s) 875, 918
MaeIII GTNAC 2 cut(s) 484, 886
MalI GATC 5 cut(s) 171, 664, 819, 935, 948
MboI GATC 5 cut(s) 169, 662, 817, 933, 946
MboII GAAGA 6 cut(s) 147, 313, 316, 350, 845, 861
MhlI GDGCHC 2 cut(s) 380, 772
MluCI AATT 4 cut(s) 322, 422, 552, 698
MlyI GAGTC 4 cut(s) 161, 203, 221, 796
MmeI TCCRAC 3 cut(s) 171, 390, 439
MspA1I CMGCKG 3 cut(s) 254, 437, 893
MspI CCGG 1 cut(s) 359
MspR9I CCNGG 1 cut(s) 319
Mva1269I GAATGC 2 cut(s) 558, 847
MvaI CCWGG 1 cut(s) 319
MwoI GCNNNNNNNGC 2 cut(s) 251, 575
NdeII GATC 5 cut(s) 169, 662, 817, 933, 946
NlaIII CATG 4 cut(s) 532, 680, 709, 833
NlaIV GGNNCC 2 cut(s) 379, 542
NmuCI GTSAC 1 cut(s) 484
PaqCI CACCTGC 1 cut(s) 266
PctI GAATGC 2 cut(s) 558, 847
PfeI GAWTC 2 cut(s) 5, 913
PkrI GCNGC 3 cut(s) 439, 610, 784
PleI GAGTC 4 cut(s) 160, 202, 220, 795
PpsI GAGTC 4 cut(s) 160, 202, 220, 795
PpuMI RGGWCCY 1 cut(s) 505
Psp5II RGGWCCY 1 cut(s) 505
Psp6I CCWGG 1 cut(s) 317
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 2 cut(s) 379, 542
PspPI GGNCC 4 cut(s) 505, 541, 855, 896
PspPPI RGGWCCY 1 cut(s) 505
PstI CTGCAG 1 cut(s) 165
PstNI CAGNNNCTG 1 cut(s) 488
PvuII CAGCTG 2 cut(s) 254, 893
SatI GCNGC 3 cut(s) 438, 609, 783
Sau3AI GATC 5 cut(s) 169, 662, 817, 933, 946
Sau96I GGNCC 4 cut(s) 505, 541, 855, 896
SchI GAGTC 4 cut(s) 161, 203, 221, 796
ScrFI CCNGG 1 cut(s) 319
SduI GDGCHC 2 cut(s) 380, 772
SfaNI GCATC 2 cut(s) 875, 918
SfcI CTRYAG 2 cut(s) 161, 612
SinI GGWCC 3 cut(s) 505, 541, 896
Sse9I AATT 4 cut(s) 322, 422, 552, 698
SsiI CCGC 2 cut(s) 261, 435
StyD4I CCNGG 1 cut(s) 317
TaaI ACNGT 5 cut(s) 60, 207, 490, 742, 1001
TaqI TCGA 2 cut(s) 973, 1027
TasI AATT 4 cut(s) 322, 422, 552, 698
TfiI GAWTC 2 cut(s) 5, 913
TscAI CASTG 2 cut(s) 37, 489
TseFI GTSAC 1 cut(s) 484
TseI GCWGC 3 cut(s) 437, 608, 782
Tsp45I GTSAC 1 cut(s) 484
TspDTI ATGAA 7 cut(s) 255, 351, 459, 460, 503, 846, 969
TspRI CASTG 2 cut(s) 37, 489
VpaK11BI GGWCC 3 cut(s) 505, 541, 896
XcmI CCANNNNNNNNNTGG 2 cut(s) 103, 187
XmiI GTMKAC 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.