Rh5BG375500

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011861
Physical Location & Seq
Reverse (-)
59346121 .. 59349214
3094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG375500.1

Sequence Viewer

Length: 1098 bp
ATGTTTAATCCCGTGGAAGAGAAGAACATTATGGAATCTCCCCCTATCTCTCCCCTAAGCAGTGAACAGGAGCCGAACAACAGTAAACAGTTGGTGGAGTGTCTTCCACAGTCTGACCTTATCGCCAATGAGTTGGTGGAGTTTCCTCCACAGTCTGACCCCATCGCCGATGAGTTGGTGGAGTCTTCTCTGCAGTCTAATCCCATTGCCAATGAGTTGTTGGAGTCTCCTCCACAGTCTGAGTCTGACCCCATCGCCGATGAGAACAATGAAGCACTATCAGCTGAAGAAGGCGGTCAATCAGAGGCAGGTGAAGTGATGATGACACCTAAGAAGCAGAGGGGGAGACCCAGGAATTATGAGGTCAATGAAGAAATGGTCTCTGATGTTCCAGCTTCCAACTCAGAGGGCTCCTCTAAGAAAGCAAAAGGCCGCCCCAAAGGCTCAGGCAATAAATTGCAACACCCCGCTGCCGATGAAACTGGTGGAGCTGGAAATAACTTCATCCCTTTCGCAGTGACTGTGCAGACTGGGGAGGACCTTCTTCATAAAATATGGGTCATGGCACAGAGGGACCATCAATCAATTTGCATTCTTTCGGCTACTGGTGCTGTTTCTAAACCTGTTATACGACCTAATGCTGCTGTAGGGTATGTAAAATACGAGGGTTGTTATGATATCATCAATCTCTCTGGATCGTTTGTATACCATGCAATGTATCCACACAAAAAATTGGGCATGATAACTATTCTATTGGCTAATCCCGATAATACGGTTTTTGGTGGCTCTGTTTTATCAGGAGCACCGCTCATAGCTGCGAGTCCTATTCAACTCATTCTCGGAGCTTTTGATCAAGATATACACGAAGAGTTGAATGCGAAAAGGAGGCCCTCAGTCGCATCTTCTACATACACGAGTGTAACAGCTGGTCTAGACCTTGCAAGGATTCCTGCACAAGTGATGCCGATCCAAGCAGTAGATCGGGATGAAACTCGTATTCAATATCGACGAATATCTCCATATTTCAACAACAGTCGAGATGGGACTCGTGTTCAATATCAACCAATACCTCCATATTTCAACAACAGTCTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

365

Amino Acids

39.71

Weight (kDa)

4.86

Isoelectric Point (pI)

57.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 170 - 284 4e-14 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 299
Acc36I ACCTGC 1 cut(s) 299
AccBSI CCGCTC 1 cut(s) 808
AccI GTMKAC 1 cut(s) 705
AciI CCGC 4 cut(s) 294, 433, 468, 806
AclWI GGATC 2 cut(s) 703, 961
AcuI CTGAAG 1 cut(s) 306
AgsI TTSAA 6 cut(s) 830, 874, 1001, 1027, 1055, 1081
AjnI CCWGG 1 cut(s) 350
AluBI AGCT 6 cut(s) 284, 395, 491, 815, 845, 926
AluI AGCT 6 cut(s) 284, 395, 491, 815, 845, 926
Alw21I GWGCWC 1 cut(s) 805
Alw26I GTCTC 3 cut(s) 231, 340, 385
AlwI GGATC 2 cut(s) 703, 961
AlwNI CAGNNNCTG 1 cut(s) 521
AoxI GGCC 2 cut(s) 430, 887
ApeKI GCWGC 3 cut(s) 470, 641, 815
AspS9I GGNCC 3 cut(s) 538, 574, 888
AsuHPI GGTGA 1 cut(s) 323
AvaII GGWCC 2 cut(s) 538, 574
BanII GRGCYC 1 cut(s) 413
BauI CACGAG 2 cut(s) 913, 1047
BbsI GAAGAC 3 cut(s) 95, 177, 1082
Bbv12I GWGCWC 1 cut(s) 805
BbvI GCAGC 3 cut(s) 457, 628, 802
BccI CCATC 4 cut(s) 170, 260, 585, 1034
BciT130I CCWGG 1 cut(s) 352
BciVI GTATCC 1 cut(s) 729
BclI TGATCA 1 cut(s) 850
BcoDI GTCTC 3 cut(s) 231, 340, 385
BfaI CTAG 1 cut(s) 932
BfmI CTRYAG 2 cut(s) 191, 645
BfuAI ACCTGC 1 cut(s) 299
BfuI GTATCC 1 cut(s) 729
BglI GCCNNNNNGGC 1 cut(s) 441
BisI GCNGC 4 cut(s) 433, 471, 642, 816
BlsI GCNGC 4 cut(s) 434, 472, 643, 817
Bme1390I CCNGG 1 cut(s) 352
Bme18I GGWCC 2 cut(s) 538, 574
BmgT120I GGNCC 3 cut(s) 538, 574, 888
BmiI GGNNCC 3 cut(s) 72, 412, 575
BmrFI CCNGG 1 cut(s) 352
BmrI ACTGGG 1 cut(s) 540
BmsI GCATC 2 cut(s) 908, 951
BmuI ACTGGG 1 cut(s) 540
BpiI GAAGAC 3 cut(s) 95, 177, 1082
BplI GAGNNNNNCTC 4 cut(s) 398, 430, 792, 824
Bpu10I CCTNAGC 2 cut(s) 56, 445
BsaBI GATNNNNATC 1 cut(s) 965
BsaI GGTCTC 2 cut(s) 340, 385
BsaJI CCNNGG 2 cut(s) 12, 350
Bse1I ACTGG 3 cut(s) 487, 535, 610
Bse3DI GCAATG 2 cut(s) 204, 720
Bse8I GATNNNNATC 1 cut(s) 965
BseBI CCWGG 1 cut(s) 352
BseDI CCNNGG 2 cut(s) 12, 350
BseGI GGATG 2 cut(s) 504, 991
BseJI GATNNNNATC 1 cut(s) 965
BseMI GCAATG 2 cut(s) 204, 720
BseMII CTCAG 4 cut(s) 231, 417, 459, 906
BseNI ACTGG 3 cut(s) 487, 535, 610
BseRI GAGGAG 2 cut(s) 219, 403
BseXI GCAGC 3 cut(s) 457, 628, 802
BsgI GTGCAG 2 cut(s) 545, 936
BshFI GGCC 2 cut(s) 432, 889
BsiHKAI GWGCWC 1 cut(s) 805
BslFI GGGAC 2 cut(s) 587, 1057
BsmAI GTCTC 3 cut(s) 231, 340, 385
BsmFI GGGAC 2 cut(s) 587, 1057
BsmI GAATGC 2 cut(s) 591, 880
BsnI GGCC 2 cut(s) 432, 889
Bso31I GGTCTC 2 cut(s) 340, 385
Bsp1286I GDGCHC 2 cut(s) 413, 805
Bsp143I GATC 4 cut(s) 695, 850, 966, 979
BspACI CCGC 4 cut(s) 294, 433, 468, 806
BspANI GGCC 2 cut(s) 432, 889
BspCNI CTCAG 4 cut(s) 232, 416, 458, 905
BspLI GGNNCC 3 cut(s) 72, 412, 575
BspMAI CTGCAG 1 cut(s) 195
BspMI ACCTGC 1 cut(s) 299
BspPI GGATC 2 cut(s) 703, 961
BspTNI GGTCTC 2 cut(s) 340, 385
BsrBI CCGCTC 1 cut(s) 808
BsrDI GCAATG 2 cut(s) 204, 720
BsrI ACTGG 3 cut(s) 487, 535, 610
BssECI CCNNGG 2 cut(s) 12, 350
BssMI GATC 4 cut(s) 695, 850, 966, 979
BssNAI GTATAC 1 cut(s) 706
BssSI CACGAG 2 cut(s) 913, 1047
Bst1107I GTATAC 1 cut(s) 706
Bst2BI CACGAG 2 cut(s) 913, 1047
Bst2UI CCWGG 1 cut(s) 352
Bst4CI ACNGT 9 cut(s) 83, 90, 111, 153, 237, 523, 775, 1034, 1088
Bst6I CTCTTC 2 cut(s) 12, 861
BstDEI CTNAG 8 cut(s) 56, 240, 330, 403, 417, 445, 892, 1095
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 504, 991
BstKTI GATC 4 cut(s) 698, 853, 969, 982
BstMAI GTCTC 3 cut(s) 231, 340, 385
BstMBI GATC 4 cut(s) 695, 850, 966, 979
BstMWI GCNNNNNNNGC 3 cut(s) 281, 441, 608
BstNI CCWGG 1 cut(s) 352
BstSCI CCNGG 1 cut(s) 350
BstSFI CTRYAG 2 cut(s) 191, 645
BstV1I GCAGC 3 cut(s) 457, 628, 802
BstV2I GAAGAC 3 cut(s) 95, 177, 1082
BstXI CCANNNNNNTGG 1 cut(s) 133
BstZ17I GTATAC 1 cut(s) 706
BsuI GTATCC 1 cut(s) 729
BsuRI GGCC 2 cut(s) 432, 889
BtgI CCRYGG 1 cut(s) 12
BtgZI GCGATG 2 cut(s) 148, 238
BtsCI GGATG 2 cut(s) 504, 991
BtsI GCAGTG 2 cut(s) 67, 522
BtsIMutI CAGTG 2 cut(s) 67, 522
BveI ACCTGC 1 cut(s) 299
CaiI CAGNNNCTG 1 cut(s) 521
Cfr13I GGNCC 3 cut(s) 538, 574, 888
CviAII CATG 3 cut(s) 562, 710, 739
DdeI CTNAG 8 cut(s) 56, 240, 330, 403, 417, 445, 892, 1095
DpnI GATC 4 cut(s) 697, 852, 968, 981
DpnII GATC 4 cut(s) 695, 850, 966, 979
Eam1104I CTCTTC 2 cut(s) 12, 861
EarI CTCTTC 2 cut(s) 12, 861
Eco24I GRGCYC 1 cut(s) 413
Eco31I GGTCTC 2 cut(s) 340, 385
Eco32I GATATC 1 cut(s) 679
Eco47I GGWCC 2 cut(s) 538, 574
Eco57I CTGAAG 1 cut(s) 306
EcoO109I RGGNCCY 2 cut(s) 538, 888
EcoRII CCWGG 1 cut(s) 350
EcoRV GATATC 1 cut(s) 679
EcoT38I GRGCYC 1 cut(s) 413
FaeI CATG 3 cut(s) 565, 713, 742
FaqI GGGAC 2 cut(s) 587, 1057
FatI CATG 3 cut(s) 561, 709, 738
FauI CCCGC 1 cut(s) 475
FbaI TGATCA 1 cut(s) 850
FblI GTMKAC 1 cut(s) 705
Fnu4HI GCNGC 4 cut(s) 433, 471, 642, 816
FokI GGATG 2 cut(s) 491, 998
FriOI GRGCYC 1 cut(s) 413
Fsp4HI GCNGC 4 cut(s) 433, 471, 642, 816
FspBI CTAG 1 cut(s) 932
GluI GCNGC 4 cut(s) 433, 471, 642, 816
HaeIII GGCC 2 cut(s) 432, 889
Hin1II CATG 3 cut(s) 565, 713, 742
HinfI GANTC 7 cut(s) 35, 182, 224, 242, 820, 946, 1045
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 3 cut(s) 65, 86, 706
Hpy188I TCNGA 8 cut(s) 115, 157, 241, 247, 304, 385, 406, 842
Hpy188III TCNNGA 7 cut(s) 693, 764, 798, 854, 932, 983, 1037
Hpy8I GTNNAC 3 cut(s) 65, 86, 706
Hpy99I CGWCG 1 cut(s) 1011
HpyAV CCTTC 2 cut(s) 284, 551
HpyCH4III ACNGT 9 cut(s) 83, 90, 111, 153, 237, 523, 775, 1034, 1088
HpyCH4V TGCA 7 cut(s) 193, 460, 526, 591, 713, 941, 953
HpyF10VI GCNNNNNNNGC 3 cut(s) 281, 441, 608
HpyF3I CTNAG 8 cut(s) 56, 240, 330, 403, 417, 445, 892, 1095
Hsp92II CATG 3 cut(s) 565, 713, 742
Ksp22I TGATCA 1 cut(s) 850
Kzo9I GATC 4 cut(s) 695, 850, 966, 979
LmnI GCTCC 5 cut(s) 70, 416, 488, 800, 842
Lsp1109I GCAGC 3 cut(s) 457, 628, 802
LweI GCATC 2 cut(s) 908, 951
MaeI CTAG 1 cut(s) 932
MaeIII GTNAC 2 cut(s) 517, 919
MalI GATC 4 cut(s) 697, 852, 968, 981
MbiI CCGCTC 1 cut(s) 808
MboI GATC 4 cut(s) 695, 850, 966, 979
MhlI GDGCHC 2 cut(s) 413, 805
MluCI AATT 4 cut(s) 355, 455, 585, 731
MlyI GAGTC 5 cut(s) 191, 233, 251, 829, 1039
MmeI TCCRAC 2 cut(s) 201, 423
MseI TTAA 1 cut(s) 6
MspA1I CMGCKG 3 cut(s) 284, 470, 926
MspR9I CCNGG 1 cut(s) 352
Mva1269I GAATGC 2 cut(s) 591, 880
MvaI CCWGG 1 cut(s) 352
MwoI GCNNNNNNNGC 3 cut(s) 281, 441, 608
NdeII GATC 4 cut(s) 695, 850, 966, 979
NlaIII CATG 3 cut(s) 565, 713, 742
NlaIV GGNNCC 3 cut(s) 72, 412, 575
NmuCI GTSAC 1 cut(s) 517
PaqCI CACCTGC 1 cut(s) 299
PctI GAATGC 2 cut(s) 591, 880
PfeI GAWTC 2 cut(s) 35, 946
PkrI GCNGC 4 cut(s) 434, 472, 643, 817
PleI GAGTC 5 cut(s) 190, 232, 250, 828, 1039
PpsI GAGTC 5 cut(s) 190, 232, 250, 828, 1039
PpuMI RGGWCCY 1 cut(s) 538
Psp5II RGGWCCY 1 cut(s) 538
Psp6I CCWGG 1 cut(s) 350
PspGI CCWGG 1 cut(s) 350
PspN4I GGNNCC 3 cut(s) 72, 412, 575
PspPI GGNCC 3 cut(s) 538, 574, 888
PspPPI RGGWCCY 1 cut(s) 538
PstI CTGCAG 1 cut(s) 195
PstNI CAGNNNCTG 1 cut(s) 521
PvuII CAGCTG 2 cut(s) 284, 926
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 4 cut(s) 433, 471, 642, 816
Sau3AI GATC 4 cut(s) 695, 850, 966, 979
Sau96I GGNCC 3 cut(s) 538, 574, 888
SchI GAGTC 5 cut(s) 191, 233, 251, 829, 1039
ScrFI CCNGG 1 cut(s) 352
SduI GDGCHC 2 cut(s) 413, 805
SfaNI GCATC 2 cut(s) 908, 951
SfcI CTRYAG 2 cut(s) 191, 645
SinI GGWCC 2 cut(s) 538, 574
Sse9I AATT 4 cut(s) 355, 455, 585, 731
SsiI CCGC 4 cut(s) 294, 433, 468, 806
SspMI CTAG 1 cut(s) 932
StyD4I CCNGG 1 cut(s) 350
TaaI ACNGT 9 cut(s) 83, 90, 111, 153, 237, 523, 775, 1034, 1088
TaqI TCGA 2 cut(s) 1006, 1036
TasI AATT 4 cut(s) 355, 455, 585, 731
TauI GCSGC 1 cut(s) 435
TfiI GAWTC 2 cut(s) 35, 946
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TscAI CASTG 2 cut(s) 67, 522
TseFI GTSAC 1 cut(s) 517
TseI GCWGC 3 cut(s) 470, 641, 815
Tsp45I GTSAC 1 cut(s) 517
TspDTI ATGAA 6 cut(s) 285, 384, 492, 493, 536, 1002
TspRI CASTG 2 cut(s) 67, 522
VpaK11BI GGWCC 2 cut(s) 538, 574
XbaI TCTAGA 1 cut(s) 931
XcmI CCANNNNNNNNNTGG 2 cut(s) 133, 217
XmiI GTMKAC 1 cut(s) 705
XspI CTAG 1 cut(s) 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.